The roothairs are important components of the root for absorbing nutrients for plants and also secreting the plant-produced secretes. Morphology and their number are influenced by various environmental and internal factors and are regulated by them. Mycorrhizal fungi are established through the root and their presence in the root can affect root’s physical and chemical properties.The aim of this study was to evaluate the effect of the presence of the fungus in the roots on the characteristics of the capillary roots.In order to remove the effect of other microorganisms on the results of the experiment, this experiment was performed in vitro on the roots of the carrot secondary phloem tissue culture. In this experiment, the fungus could affect the characteristics of the root hairs: their number (22% decrease in the root hairs number in the root hair area of the root) and their length (A decrease of 21.3% in the length of capillary roots in mycorrhizal plants). These changes in the characteristics of capillary roots were also caused by the presence of fungal structures in the roots as well as by the decrease in the production of strigolactones. In this study, changes in the production of strigolactones calculated by using their effect on seed germination of Phelipancheaegyptiaca.
The ethanol extracts of Ficus asperifolia, Mormordica charantia, Anacardium
occidentals and Psidium guajava were evaluated sole and in treatment combinations at 25, 50 and
75mg ml-1 concentration levels against the mycelial growth of Macrophomina phaseolina of
Cowpea. The pathogen was cultured on plates containing botanicals amended Potato Dextrose
Agar (PDA) in three replicates while only ethanol treated PDA tested plates served the control
experiment. The radial growths were recorded at 4th, 6th and 8th day after inoculation. Data
obtained were analysed using the SAS software program version 9.2. The extract of Mormordica
charantia was the most effective in the botanical treatments alone. The most significant inhibition
of Macrophomina phaseolina were observed from the combined treatments of Ficus asperifolia,
Mormordica charantia and Anacardium occidentals (3.11 cm), followed by Mormordica
charantia and Psidium guajava (3.29 cm), then combination of four extracts; Ficus asperifolia,
Mormordica charantia, Anacardium occidentals and Psidium guajava (3.53 cm), then
Mormordica charantia and Anacardium occidentals (3.84 cm). Other treatments, either alone or in
combination produced significant result compared to the control experiment (6.94 cm). However,
the efficacy of botanicals increased with concentration and also significantly correlated with time
and reduction in mycelia extension of the pathogen. More so, variability in the antifungicidal
potentials of the botanicals on Macrophomina phaseolina ranges from 15.93% to 34.06%
according to Eigen proportions. The treatment combinations of; Ficus asperifolia, Mormordica
charantia and Anacardium occidentals at 75mg ml-1 concentration level produced the most
inhibitory effect against Macrophomina phaseolina in vitro. However, the untreated plates did not
show inhibitory effect on the mycelial growth of the pathogen. Therefore, combined treatments of
botanicals could be a potential source in the practice of plant disease control.
Genetic characterization of morphological and yield traits in ten genotypes of Celosia argentea L. was evaluated
at the Research Farm of the Department of Botany, University of Ibadan, Nigeria. The experiment was laid out
in a randomized complete block design with four replicates. The results of analysis of variance carried out on
early morphological characters of C. argentea L. at 3, 4, and 5weeks after sowing showed significant
(p<0.05 /><0.01) effects except for number of leaves per plant and leaf width at 3 and 5 weeks after sowing,
respectively. The replicates in blocks produced varying observable effects on the genotypes while genotype x
replicate showed significant variation on morpho-agronomic and yield traits except number of days to flowering
at 50 days and fruit length at maturity. Also, from the result of the mean separation, it is shown that
NG/MAY/09/015 performed the best for plant height at flowering, leaf length at flowering, leaf width at
flowering, and root biomass. NG/SA/07/213 produced the highest mean values of number of flowers per plant,
leaf biomass and pod weight at maturity. The highest values of number of primary branches and fruit length at
maturity (FLM) were observed for NG/TO/MAY/09/015, while NG/AO/MAY/09/015 had the highest for pod
weight at maturity. The result of principal component axis also showed that Prin 1 accounted for highest Eigen
Vector of 38.62% from the total variation. NG/MAY/09/015 (R2) genotype produced the highest Eigen Vector
of 6.705 from Prin 1. The correlation result showed that plant height had a significant positive association with
seed weight at maturity, pod weight at maturity, number of primary branches and fruit length at maturity, while
similar association existed between leaf biomass, number of primary branches and pod weight at maturity, as
well as between plant height at flowering and pod weight at maturity. Again, the number of primary branches is
also positive and significantly correlated with plant height, root biomass and leaf length. Furthermore, the
results of dendrogram and minimum spanning tree revealed variations in genetic relatedness and distance,
respectively, which exist among the population of the C. argentea L.
Sixteen genotypes of maize were evaluated for genetic variability and character associations in the Research
Farm of the Department of Botany, University of Ibadan, Nigeria for 90 days in the 2012, 2013 and 2014
cropping seasons. Perforated polythene bags (18 × 9 cm) were filled with 6kg sandy-loam soil and were spaced
at a distance of 10 cm apart between rows. Three seeds from each genotype were then planted per bag without
treatment in three replicates. Data on plant and stem height, leaf width, leaf length and number of leaves were
collected after planting at 14 days interval on each replicate for a particular genotype. The plant height of
EVDT.Y200STRQPM genotype was significantly (p < 0.05) different from other genotypes, while genotype
pairs; TZE-OR2DTSTRQPM and 2009TZE-ORIDTSTRQPM, TZEI 22, TZEI 98 and OBANTAPA, TZEI 4,
TZEI 161, BODIJA as well as EVDT-W99STR, OJO, 98SYNWECSTRQPM were similar to each other. The
result of correlation coefficient shows that stem height was highly significant and positively correlated with the
plant height (p < 0.01; r = 0.91). The genotype is negative and non-correlated with plant height, stem height, leaf
width and number of leaves, but positive and non-significantly associated with leaf length, replicate and week
after planting.
Application of arbuscular mycorrhizal fungi accelerates the growth of shoot r...UniversitasGadjahMada
This document summarizes a study that examined the effects of applying different doses of arbuscular mycorrhizal fungi (AMF) inoculum on shoot root growth of five sugarcane clones. The key findings are:
1) Application of 2-3 g of AMF inoculum/bud chips resulted in faster and greater root colonization compared to the control, reaching 57-100% colonization within 5 days.
2) AMF inoculation significantly increased shoot root traits like root length, surface area, and number of shoot roots, especially for clones BL, VMC, and PS864.
3) AMF application of 2-3 g/bud chips also significantly increased seedling
Effects of β-cyclodextrin on in vitro rooting and bulbing of lilium (Lilium l...Premier Publishers
The auxins liability often limits expected effects on in vitro culture of plant tissues. In this regard, β-cyclodextrine was used as protective substance to auxins in order to improve rooting and in vitro bulbing of lily (Lilium longiflorum L.). This oligosaccharide was added at a concentration of 10, 20 and 30 mg/l to the rooting medium containing mineral and vitamin additives of Murashige and Skoog (MS), 1.5 mg/l of indole-3-butyric acid (IBA), 30 g/l of sucrose and 6 g/l of agar. After 30 days of culture, in order to enhance in vitro bulbe formation, a liquid medium supplemented with 6-benzylaminopuine (BAP) was added to form a double phase (solid / liquid) media. The results obtained showed that β-cyclodextrin improves the rooting of lily in vitro shoots. Indeed, the average number of roots increases from 5.64 to 7.20 roots per vitro-plant in the medium supplemented with β-cyclodextrin and root length increased from 1.72 to 2.2 cm compared to control.
Cotton Sucking Pests and Measures to Combat with Themijtsrd
This article describes the morphology, biology and pest control methods of cotton sucking pests. Aziza Djumaeva Numondjon Kizi | Akmal Musaev Anvar Ugli | Ulugbek Bakhodirov Zokirjon Ugli "Cotton Sucking Pests and Measures to Combat with Them" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Special Issue | Modern Trends in Scientific Research and Development, Case of Asia , October 2020, URL: https://www.ijtsrd.com/papers/ijtsrd37938.pdf Paper Url :https://www.ijtsrd.com/engineering/agricultural-engineering/37938/cotton-sucking-pests-and-measures-to-combat-with-them/aziza-djumaeva-numondjon-kizi
Effect of interaction between different plant growth regulators on in vitro s...Agriculture Journal IJOEAR
— In this paper a shoot multiplication is described for Citrus latifolia Tan. (persian lime) using nodal segment explants of young one – old – year trees by two different pathways contain with and without callusing phase. The best result for multiple shoot formation and regenerated shoot formation was 3.2 and 2.6 shoots per explants with 4.44 µM BA plus 0.053 µM NAA and 4.44 µM BA plus 0.049 µM IBA respectively. Alike shoot regeneration, shoot elongation was occurred in medium with 4.44 µM BA and 0.049 µM IBA. Micropropagated and regenerated plants are under other experiments. Abbreviation: BA – 6 benzylaminopurine; IBA – Indole acetic acid; NAA – Naphtalene acetic acid; PGRs – Plant Growth Regulators.
New generation plant growth regulators in fruit cropsAsish Benny
The document summarizes new generation plant growth regulators and their effects in fruit crops. It discusses brassinosteroids, salicylic acid, jasmonates, peptides, and polyamines. Brassinosteroids promote growth, yield, quality and stress tolerance in many fruits. Salicylic acid increases yield and quality in strawberry, pomegranate, orange and mango. Jasmonates enhance growth and resistance to disease in banana. Peptides like acetyl thioproline increase papaya yield. Polyamines improve mycorrhizal colonization and growth in apple seedlings. These new generation regulators show potential to improve fruit cultivation.
The ethanol extracts of Ficus asperifolia, Mormordica charantia, Anacardium
occidentals and Psidium guajava were evaluated sole and in treatment combinations at 25, 50 and
75mg ml-1 concentration levels against the mycelial growth of Macrophomina phaseolina of
Cowpea. The pathogen was cultured on plates containing botanicals amended Potato Dextrose
Agar (PDA) in three replicates while only ethanol treated PDA tested plates served the control
experiment. The radial growths were recorded at 4th, 6th and 8th day after inoculation. Data
obtained were analysed using the SAS software program version 9.2. The extract of Mormordica
charantia was the most effective in the botanical treatments alone. The most significant inhibition
of Macrophomina phaseolina were observed from the combined treatments of Ficus asperifolia,
Mormordica charantia and Anacardium occidentals (3.11 cm), followed by Mormordica
charantia and Psidium guajava (3.29 cm), then combination of four extracts; Ficus asperifolia,
Mormordica charantia, Anacardium occidentals and Psidium guajava (3.53 cm), then
Mormordica charantia and Anacardium occidentals (3.84 cm). Other treatments, either alone or in
combination produced significant result compared to the control experiment (6.94 cm). However,
the efficacy of botanicals increased with concentration and also significantly correlated with time
and reduction in mycelia extension of the pathogen. More so, variability in the antifungicidal
potentials of the botanicals on Macrophomina phaseolina ranges from 15.93% to 34.06%
according to Eigen proportions. The treatment combinations of; Ficus asperifolia, Mormordica
charantia and Anacardium occidentals at 75mg ml-1 concentration level produced the most
inhibitory effect against Macrophomina phaseolina in vitro. However, the untreated plates did not
show inhibitory effect on the mycelial growth of the pathogen. Therefore, combined treatments of
botanicals could be a potential source in the practice of plant disease control.
Genetic characterization of morphological and yield traits in ten genotypes of Celosia argentea L. was evaluated
at the Research Farm of the Department of Botany, University of Ibadan, Nigeria. The experiment was laid out
in a randomized complete block design with four replicates. The results of analysis of variance carried out on
early morphological characters of C. argentea L. at 3, 4, and 5weeks after sowing showed significant
(p<0.05 /><0.01) effects except for number of leaves per plant and leaf width at 3 and 5 weeks after sowing,
respectively. The replicates in blocks produced varying observable effects on the genotypes while genotype x
replicate showed significant variation on morpho-agronomic and yield traits except number of days to flowering
at 50 days and fruit length at maturity. Also, from the result of the mean separation, it is shown that
NG/MAY/09/015 performed the best for plant height at flowering, leaf length at flowering, leaf width at
flowering, and root biomass. NG/SA/07/213 produced the highest mean values of number of flowers per plant,
leaf biomass and pod weight at maturity. The highest values of number of primary branches and fruit length at
maturity (FLM) were observed for NG/TO/MAY/09/015, while NG/AO/MAY/09/015 had the highest for pod
weight at maturity. The result of principal component axis also showed that Prin 1 accounted for highest Eigen
Vector of 38.62% from the total variation. NG/MAY/09/015 (R2) genotype produced the highest Eigen Vector
of 6.705 from Prin 1. The correlation result showed that plant height had a significant positive association with
seed weight at maturity, pod weight at maturity, number of primary branches and fruit length at maturity, while
similar association existed between leaf biomass, number of primary branches and pod weight at maturity, as
well as between plant height at flowering and pod weight at maturity. Again, the number of primary branches is
also positive and significantly correlated with plant height, root biomass and leaf length. Furthermore, the
results of dendrogram and minimum spanning tree revealed variations in genetic relatedness and distance,
respectively, which exist among the population of the C. argentea L.
Sixteen genotypes of maize were evaluated for genetic variability and character associations in the Research
Farm of the Department of Botany, University of Ibadan, Nigeria for 90 days in the 2012, 2013 and 2014
cropping seasons. Perforated polythene bags (18 × 9 cm) were filled with 6kg sandy-loam soil and were spaced
at a distance of 10 cm apart between rows. Three seeds from each genotype were then planted per bag without
treatment in three replicates. Data on plant and stem height, leaf width, leaf length and number of leaves were
collected after planting at 14 days interval on each replicate for a particular genotype. The plant height of
EVDT.Y200STRQPM genotype was significantly (p < 0.05) different from other genotypes, while genotype
pairs; TZE-OR2DTSTRQPM and 2009TZE-ORIDTSTRQPM, TZEI 22, TZEI 98 and OBANTAPA, TZEI 4,
TZEI 161, BODIJA as well as EVDT-W99STR, OJO, 98SYNWECSTRQPM were similar to each other. The
result of correlation coefficient shows that stem height was highly significant and positively correlated with the
plant height (p < 0.01; r = 0.91). The genotype is negative and non-correlated with plant height, stem height, leaf
width and number of leaves, but positive and non-significantly associated with leaf length, replicate and week
after planting.
Application of arbuscular mycorrhizal fungi accelerates the growth of shoot r...UniversitasGadjahMada
This document summarizes a study that examined the effects of applying different doses of arbuscular mycorrhizal fungi (AMF) inoculum on shoot root growth of five sugarcane clones. The key findings are:
1) Application of 2-3 g of AMF inoculum/bud chips resulted in faster and greater root colonization compared to the control, reaching 57-100% colonization within 5 days.
2) AMF inoculation significantly increased shoot root traits like root length, surface area, and number of shoot roots, especially for clones BL, VMC, and PS864.
3) AMF application of 2-3 g/bud chips also significantly increased seedling
Effects of β-cyclodextrin on in vitro rooting and bulbing of lilium (Lilium l...Premier Publishers
The auxins liability often limits expected effects on in vitro culture of plant tissues. In this regard, β-cyclodextrine was used as protective substance to auxins in order to improve rooting and in vitro bulbing of lily (Lilium longiflorum L.). This oligosaccharide was added at a concentration of 10, 20 and 30 mg/l to the rooting medium containing mineral and vitamin additives of Murashige and Skoog (MS), 1.5 mg/l of indole-3-butyric acid (IBA), 30 g/l of sucrose and 6 g/l of agar. After 30 days of culture, in order to enhance in vitro bulbe formation, a liquid medium supplemented with 6-benzylaminopuine (BAP) was added to form a double phase (solid / liquid) media. The results obtained showed that β-cyclodextrin improves the rooting of lily in vitro shoots. Indeed, the average number of roots increases from 5.64 to 7.20 roots per vitro-plant in the medium supplemented with β-cyclodextrin and root length increased from 1.72 to 2.2 cm compared to control.
Cotton Sucking Pests and Measures to Combat with Themijtsrd
This article describes the morphology, biology and pest control methods of cotton sucking pests. Aziza Djumaeva Numondjon Kizi | Akmal Musaev Anvar Ugli | Ulugbek Bakhodirov Zokirjon Ugli "Cotton Sucking Pests and Measures to Combat with Them" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Special Issue | Modern Trends in Scientific Research and Development, Case of Asia , October 2020, URL: https://www.ijtsrd.com/papers/ijtsrd37938.pdf Paper Url :https://www.ijtsrd.com/engineering/agricultural-engineering/37938/cotton-sucking-pests-and-measures-to-combat-with-them/aziza-djumaeva-numondjon-kizi
Effect of interaction between different plant growth regulators on in vitro s...Agriculture Journal IJOEAR
— In this paper a shoot multiplication is described for Citrus latifolia Tan. (persian lime) using nodal segment explants of young one – old – year trees by two different pathways contain with and without callusing phase. The best result for multiple shoot formation and regenerated shoot formation was 3.2 and 2.6 shoots per explants with 4.44 µM BA plus 0.053 µM NAA and 4.44 µM BA plus 0.049 µM IBA respectively. Alike shoot regeneration, shoot elongation was occurred in medium with 4.44 µM BA and 0.049 µM IBA. Micropropagated and regenerated plants are under other experiments. Abbreviation: BA – 6 benzylaminopurine; IBA – Indole acetic acid; NAA – Naphtalene acetic acid; PGRs – Plant Growth Regulators.
New generation plant growth regulators in fruit cropsAsish Benny
The document summarizes new generation plant growth regulators and their effects in fruit crops. It discusses brassinosteroids, salicylic acid, jasmonates, peptides, and polyamines. Brassinosteroids promote growth, yield, quality and stress tolerance in many fruits. Salicylic acid increases yield and quality in strawberry, pomegranate, orange and mango. Jasmonates enhance growth and resistance to disease in banana. Peptides like acetyl thioproline increase papaya yield. Polyamines improve mycorrhizal colonization and growth in apple seedlings. These new generation regulators show potential to improve fruit cultivation.
This document discusses using in vitro mutagenesis techniques to induce desirable mutations in flower crops. It describes mutagenesis as a process that changes genetic information, resulting in mutations. Both physical (radiation) and chemical (EMS, DES) mutagens are used. The document presents case studies on inducing mutations in tuberose and rose plants using gamma rays and chemical mutagens. Desirable variations were observed, such as changes in flower shape, color, and stem length. Radiation doses of 40 Gy and 55 Gy were found to successfully induce novel variants in rose. The objective is to develop improved varieties of ornamental plants using these mutation techniques.
ABSTRACT- The present study describes the hormonal regulation on morphogenesis in vitro in nodal segments of T. indica. The nodal explants, sterilized with 0.1% HgCl2, were cultured on Murashige and Skoog Medium enriched with various combinations and concentrations of plant hormones auxin and cytokinin to study the hormonal regulation on morphogenesis in vitro in T. indica. BAP at high concentration could not evoke any morphogenetic response in nodal explants. Calli formation at the basal part of nodal explants were noted on medium containing BAP (0.1 mg/L) and 2,4-D (5.0 mg/L). 0.1 mg/L BAP was found most effective in the shoot development of the T. indica. Rhizogenesis was observed on half-strength MS medium supplemented with 1.0 mg/l IAA and 0.1 mg/l NAA. The study may also be used mass-propagation and conservation of this medicinal plant species.
Key-words- Plant growth regulators, Morphogenesis in vitro, Tylophora indica, Rhizogenesis
Influence of water stress and rhizobial inoculation on growth and yield of se...Innspub Net
Two season’s field experiment and single season screen house experiment were conducted to assess the effect of water stress periods and rhizobial inoculation in five P. vulgaris cultivars. The experiment consisted of two levels of rhizobia (with and without inoculation), two stress levels (with and without water stress) and five cultivars of P. vulgaris (KAT B9, KAT B1, F9 Kidney Selection, F8 Drought line and JESCA). Results showed that rhizobial inoculation significantly increased plant height (cm), leaf area (cm2), shoot and root dry weight (g-1 plant) and seed yields (kg-1 ha) at vegetative and flowering in field experiment. Furthermore, water stress treatments significantly reduced plant height (cm), stem diameter (mm), shoot and root dry weight (g-1 plant) and seed yields (kg-1 ha) in both growth stages at field experiment. For screen house experiment rhizobial inoculation significantly increased leaf area (cm2), number of leaves, stem girth (mm), shoot and root dry weight (g-1 plant) at both growth stages. Additionally, water stress treatments significantly reduced number of leaves, stem diameter (mm), shoot and root dry weight (g-1 plant) in both growth stages. Varieties F9 Kidney Selection, F8 Drought Line and JESCA had significantly superior measurements reflected in increased plant height (cm), shoot and root dry weight (g-1 plant) and seed yields (kg-1 ha) as compared with KAT B9 and KAT B1. Furthermore, significant interactive effects were also seen between rhizobial inoculation x stress level and tested bean cultivars on plant height, number of leaves, stem diameter, shoot dry weight and seed yields.
Radiosensitivity and Seedling Growth of Several Genotypes of Paddy Rice Mutan...AI Publications
Researchers use mutation induction in rice to create high genetic diversity. The basic population with high genetic diversity will facilitate the selection process for the desired good characters. This study aimed to determine the optimal dose that induces the highest genetic diversity in four lowland rice genotypes. The research materials were four genotypes of lowland rice, namely “G10”, “G16”, “Baas Selem”, and “Inpago Unram-1”. Gamma irradiation was carried out at the Center for Isotope and Radiation Application (PAIR) BATAN. Each genotype was irradiated at doses of 200, 300, 400 and 500 Gy. The seeding is done in the glasshouse of the Faculty of Agriculture, University of Mataram. Observations were made on the number of growing seeds, plant height and number of leaves. The LD50 value was determined based on the results of the regression analysis of the number of growing seeds at the four irradiation doses plus control (0 Gy). The results indicated that (1) the numbers of growing seeds decreased as the doses of gamma irradiation increased, (2) the LD50 value of the four rice genotypes ranged from 264 to 518 Gy, (3) the optimal dose of gamma ray irradiation for G10, G16, Baas Selem, and Inpago Unram-1 were 264 Gy, 398 Gy, 316 Gy and 518 Gy, respectively. (4) Among the four rice genotypes teasted, “G10” mutant was the most sensitive to gamma ray irradiation, whereas” Inpago Unram-1” mutant was the least sensitive genotype.
Use of nanofertilizers on fruit trees contributes effectively to improve the fruit quality and increasing the productivity of trees. It reduces environmental pollution by reducing the amount of fertilizers used, which is positively reflected in the increased economic return of the farmers. When nanofertilizers sprayed at very low concentration on fruit trees, these compounds have had a direct effect by increasing the growth, yield and quality of these fruit crops.
Influence of foliar fertilizing on stomata parameters in maize leaf (Zea mays...Innspub Net
In this research, the effects of foliar fertilizer Megegreen on stomata parameters of maize leaf (ZP 677) were studied. The experiment was performed on the experimental fields of the Institute of Agriculture, in Skopje, R. of Macedonia, during the 2008 and 2009. The foliar fertilizer was applied four times during the growing period in different concentrations of 0.3, 0.6 and 0.9% solution. Stomata density and size were measured on the adaxial and abaxial leaf surface from randomly selected plants from each replication in stage of silking. Stomata counts were made on the impressions from microscopic fields using the colodium method. Analyses of variance indicated that the application of foliar fertilizer has significant influence on stomata features on corn leaves. Results from research, show higher stomata density on adaxial (176,19-182,32 stomata /mm2) and abaxial surface (289,12-293,12 stomata/mm2) at variants 3 and 4. Variant 3 has the highest stomata length on adaxial surface (59,75 μm), without significant difference and the highest average length on the abaxial surface (63,00 μm), which is significantly different from the control variant. With the highest average width on adaxial leaf surface was variant 4 with 11,56 μm and on the abaxial surface was variant 2, with 13,49 μm. A positive significant correlation was observed between stomata number on the adaxial and abaxial surface of leaf (R2= 0,856**). Get the full articles at: http://www.innspub.net/volume-7-number-4-october-2015-ijaar/
Evaluating Kenyan Dolichos (Lablab purpureus L.) Genotypes for Resistance to ...Premier Publishers
The objective of this study was to evaluate eighteen Dolichos lablab genotypes for resistance to Maruca vitrata and Helicoverpa armigera in the field using morphological markers. The study design was Randomized Complete Block (RCBD) with separation of mean done using Turkey’s range of test. Eldoret, KALRO Njoro and KALRO Kakamega were the study sites. Morphological parameters of pods were studied to determine whether they have any influence on resistance of Dolichos lablab to M. vitrata and H. armigera. The pod damage (%) of each genotype was calculated and given a resistance rating of 1-5 score damage. Genotype G2, Bahati and W7 were resistant to M. vitrata in a scale of 1(0-10%; low infestation), Bahati and W7 were moderately resistant to H. armigera in a scale of 2(11-30%; moderate infestation). Genotype LG1MoiP10 was susceptible to M. vitrata in a scale of 4 (51-70%; severe infestation) and genotype M5 was intermediate to H. armigera in a scale of 3 (31-50%; high infestation). There was positive significant correlation in H. armigera and M. vitrata pod damage with days to maturity, growth habit, and pod attachment. Pod length and pod fragrance were positively correlated to M. vitrata. Negative correlation was detected in pod thickness, pod pubescence and raceme position to pod damage by H. armigera and M. vitrata. The study identified G2, Bahati and W7 as promising resistant genotypes and can be used in Dolichos breeding program. However, there is need to further evaluate them in different environments and seasons for reliability.
Growth analysis of rhizomania infected and healthy sugar beetJavad Rezaei
This study analyzed the growth of rhizomania-tolerant and susceptible sugar beet cultivars grown under infested and non-infested soil conditions. Results showed that under infested soil, susceptible cultivars produced 57% less root dry matter and 24% lower leaf area index than tolerant cultivars. Growth rates including crop growth rate and net assimilation rate were also lower for susceptible cultivars under infested conditions compared to tolerant cultivars. However, on non-infested soil, there were no significant differences in dry matter or growth indices between susceptible and tolerant cultivars. The study quantified the effects of rhizomania infection on sugar beet growth to provide data for modeling rhizomania impacts
This document discusses genetic diversity analysis of grape (Vitis vinifera L.) germplasm in India using microsatellite markers. A total of 42 grape genotypes were analyzed using 7 microsatellite markers. A total of 45 alleles were detected among the genotypes. The microsatellites grouped the genotypes into two main clusters (A and B) based on morphological and genetic characteristics, with subclusters differentiating seeded vs seedless fruits and pigmented vs non-pigmented fruits. The study found high genetic variability among the Indian grape germplasm and that microsatellite markers are a reliable tool for diversity and breeding programs.
Productivity of cassava sweet potato intercropping system as influenced by va...Alexander Decker
This study evaluated the productivity of a cassava-sweet potato intercropping system using different lengths of cassava cuttings in Makurdi, Nigeria over two growing seasons. Cassava cuttings of 20cm, 30cm, and 40cm lengths were intercropped with sweet potato. The highest yields for both crops were obtained when cassava cuttings were 30cm long, with land equivalent ratios of 2.15, indicating 53.5% higher land use efficiency than sole cropping. Intercropping cassava cuttings of 30cm length resulted in the greatest vegetative growth and highest yields for both crops, making it the most suitable combination for intercropping cassava and sweet potato.
Assessment of forage corn quality intercropping with green beans under influe...Innspub Net
To assess the quality of forage corn intercropping with green beans under the influence of Rhizobium bacteria and Arbuscular Mycorrhizal fungus, make a test in educational-research farm of agriculture faulty of Azna PNU that it was design in factorial to randomized complete block with three replications. The experimental factors include cropping systems such as mono cropping of corn, mono cropping of green beans, intercropping, Arbuscular Mycorrhizal fungus (use and non-use) and Rhizobium bacteria (use and non-use). The results showed that cropping systems on crude protein, wet forage weight, dry forage weight were significant at 1% level as well as leaf to stem ratio was significant at 5% level. Between different levels of bacteria used, acid detergent fiber was impressed and was significant at 5% level. Arbuscular Mycorrhizal fungus was significant at 5% level on water soluble carbohydrate. The results showed that the use of separate and combined of Rhizobium bacteria and Arbuscular Mycorrhizal fungus increase the quality of corn in intercropping than mono cropping. Finally with increasing of plant diversity and microorganism in soil increased the quality and quantity of forage. Get the full articles at: http://www.innspub.net/volume-6-number-5-may-2015-jbes/
No 12. impact of ga3 and naa on horticultural traits of abelmoschus esculentus.PARTNER, BADC, World Bank
This study investigated the effects of gibberellic acid (GA) and naphthalene acetic acid (NAA) on growth and yield of okra. GA and NAA were applied at 50 ppm to okra plants. GA treatment resulted in the tallest plants, most leaves, longest petioles, highest fresh and dry weights, earliest flowering, most flower buds and pods, and highest yield compared to NAA and control treatments. Specifically, GA treatment yielded 338.1 g per plant, 2.9 kg per plot, and 16.4 tons per hectare, outperforming the other treatments. The results suggest that GA has potential to increase growth and yield of okra more effectively than N
Comparison of Growth and Development of Pleurotus florida against Wastes from...Dr. siddhant
Two wastes from animal origin, viz., human hairs and egg shells were evaluated for different manifestations of white oyster mushroom, Pleurotus florida. The mushroom utilized both the
substrates for their growth and sporophore formation. The mycelial growth was significantly (P=0.05) faster on egg shell (18 days) as compared to human hair (23 days). The crop of mushroom was harvested in four flushes where human hairs showed higher yield and biological efficiency of mushroom (165 gm, 33%) than egg shells (155 gm, 31%), respectively. In respect of yield parameters such as yield, biological efficiency, number of mushroom fruit bodies and average weight of
sporophores, both the substrates were statistically at par to each other. Utilization of human hairs egg shells by P. florida reveals a new strategy for mycoremediation of these wastes.
Germination percentage and growing behavior of Salix tetrasperma (Willow) as ...Innspub Net
Propagation through branch cuttings is one of the best methods to produced tree nursery the yield of the tree produced from a cutting can be higher then a tree produced from seedlings, the conditions provided to them are important factor for getting good results. The aim of this study was to find the best size of cuttings for establishment of nursery and also to compare their performance in open air or in artificial conditions under plastic sheets cuttings of 2 inches, 4 and 6 of Salix tetrasperma were raised in plastic bags of size 3x7 their were three treatments with 25 bags in each and replicated 4 times. The data on sprouting percentage, plant height, root development etc was recorded after every two weeks. The data thus collected was analyzed statically using randomized complete block design. Result showed that cutting of 2 inches has high sprouting percentage and growth behavior as compared to other cuttings. Get more articles at: http://www.innspub.net/volume-6-number-4-april-2015-jbes/
Mutation Induction for Improvement of Banana (Musa Spp). "Berangan Cv. Intan-...paperpublications3
Abstract: In vitro mutation induction by using gamma ray at 20, 30, 40 and 60Gy was used to generate variability in triploid banana "Berangan cv. Intan (AAA), so as to provide the opportunity to select plants with desirable characters such as early fruiting and short stature. Mutation frequency increased with increased dosage whereas, survival and capacity to regenerate decreased with increased doses. Time to initiation varied from 4-8 weeks for gamma-irradiated materials compared to 2-3 weeks in the control. It appeared that the higher the dose, the longer it took for shoot initiation. The exposure of shoot-tip meristem pieces to radiation doses produced wide variation in growth and morphogenetic performance. Mutagenic treatments induced 2 to 3- fold increases in variability in both quantitative and qualitative traits at different stages, in vitro; at nursery and field. For the field-grown plants; the proportion varied from 2.9% for the control plants to 16.8% for 60 Gy and 20.1% for 40 Gy-treatments, while for treatments at 20 and 30 Gy variations was at 18% and 19.2%, respectively. The frequency of variants was highest in 40 Gy followed by 30 Gy and 20 Gy, while it was very low in 60 Gy except for plant stature (dwarfism or stunted growth). Earliness to flowering variants were recorded at 20, 30 and 40 Gy at low frequencies (0.6%, 0.7% and 1.7%) respectively, while none was observed for 60 Gy treatments. As in vitro mutation induction could create genetic variability as well as many undesirable variants, it is highly desirable to integrate in vitro mutation with a selection system that can screen for large mutagen treated population. The useful variants recorded for earliness to flowering were selected for 30 and 40 Gy treatments. 40 Gy showed high frequency in earliness as compared with 20 and 30 Gy., which came to flowering as early as 6 – 6.5 months compared to 7-8 months for control. The useful dwarf, which considered as desirable traits, showed a balance of height and girth.
Criteria of Most Efficient EcM Strains for Afforestation and Reforestation Pr...Muhammad Usman Mughal
This document discusses criteria for selecting the most efficient ectomycorrhizal (ECM) fungal strains for use in afforestation and reforestation programs. It defines afforestation and reforestation and introduces key selection criteria like compatibility with host plants, growth promotion effects, persistence in soil. Experimental methods are described for testing fungal isolates like glasshouse trials. A sample experiment shows Pisolithus tinctorius promoted growth in Eucalyptus species. Tables list efficient ECM fungi identified for various tree species. The use of ECM fungi in afforestation/reforestation programs and reasons for potential failures in field plantings are also summarized.
The effect of dosage of mycorrhizal Glomus mosseae and some varieties on grow...Innspub Net
This study aims to determine the effect of Glomus mosseae mycorrhizae dose and varieties on growth and yield of chili (Capsicum annum. L) on Entisol soil. This research was conducted at the UNSYIAH Experimental Garden, Banda Aceh. The design used in this study is factorial randomized block design (RBD). The first factor in this study is the dose of mycorrhizal Glomus mosseae (D) consisting of 4 levels, namely: D0= without mycorrhiza, D1= mycorrhiza Glomus mosseae 5g plant-1, D2= mycorrhiza Glomus mosseae 10g plant-1, and D3= mycorrhiza Glomus mosseae 15g plant-1. The second factor is chili variety (V) consisting of 3 levels, namely: PM 999 varieties, Lado F1 varieties, varieties CTH-01. The variables observed were growth parameters, chili yield, total N-content and P-available on Entisol soil and the percentage of mycorrhizal infected roots. The results showed that mycorrhizal doses were able to increase the value of N-total and P-available on the soil and can improve plant growth and yield, the use of mycorrhizal doses of 15 g tan-1 generally results in better growth and yield of chilli compared to other doses, while based on the results of research CTH-01 varieties affect the growth and yield of chili plants. There is an interaction between mycorrhizal doses with chilli varieties on growth parameters and chili yield.
High ambient temperature is the greatest problem faced by tropical greenhouse producing
vegetables. Temperature affects the productivity and growth of a plant, therefore using shades to alleviate this
effect could increase the yield of plants grown in greenhouses. This study intends to investigate the effect of
shading and greenhouse conditions on brinjal flowering and traits, and subsequently compare them to open
field cultivation of brinjal.
Evaluation of fungicides for their efficacy against seed-borne fungi of Groun...ICRISAT
Groundnut germplasm exchange has played a significant role in crop improvement programmes by providing wide genetic diversity world over. However, there is an inherent risk of introduction of new exotic pathogens or new/more virulent races of a pathogen into new areas. The basic principle of plant quarantine is to regulate the movement of seed material to mitigate the associated pest risk. Salvaging, a process of disinfecting infected seed, is an important quarantine strategy to prevent the introduction of new pathogens or virulent races into new areas.
In vitro STUDIES IN Baliospermummontanum (Willd) Mull. Arg.Midhun M Nair
This document summarizes an in vitro study of Baliospermum montanum, an endangered medicinal plant. Shoot tip cultures were used to regenerate plants, with the maximum elongation occurring in medium containing BA (5mg/l) and IBA (0.4mg/l). Nodal explants showed maximum axillary bud proliferation in medium with BA (4mg/l) and IAA (0.5mg/l). Stem explants produced the most callus when cultured in medium with BA (5mg/l) and 2,4-D (5mg/l). The study aims to develop tissue culture techniques to rapidly propagate B. montanum and protect it from overexploitation
The objectives of this study are: (i): To investigate and recognize the internal and abnormalities impacts induced by phytoplasma infection in the tomato host according to recent studies have shown that the association between plants and phytoplasmas can result in anatomical alteration in phloem tissues of infected plants, and great differences between healthy and diseased samples using microscopic examination of longitudinal, cross or ultra-thin sections of leaf blade, leaf petiole and stem. (ii): To determine the efficiency of different techniques toward production of phytoplasma-free tomato plantlets and mitigation of phytoplasma disease.
Mycorrhizal induced resistance and plant innate immunityOlivia Nongthombam
This document discusses mycorrhizal induced resistance (MIR) and plant innate immunity. It describes the different types of mycorrhizal associations including arbuscular mycorrhizae. MIR occurs in four phases and involves modulation of the plant immune system through changes in hormones like jasmonic acid and salicylic acid. Arbuscular mycorrhizal fungi can prime the jasmonic acid pathway in plants to induce a stronger defense response against pathogens. Gene expression is also altered in mycorrhizal plants to produce antifungal proteins that enhance resistance.
This document discusses using in vitro mutagenesis techniques to induce desirable mutations in flower crops. It describes mutagenesis as a process that changes genetic information, resulting in mutations. Both physical (radiation) and chemical (EMS, DES) mutagens are used. The document presents case studies on inducing mutations in tuberose and rose plants using gamma rays and chemical mutagens. Desirable variations were observed, such as changes in flower shape, color, and stem length. Radiation doses of 40 Gy and 55 Gy were found to successfully induce novel variants in rose. The objective is to develop improved varieties of ornamental plants using these mutation techniques.
ABSTRACT- The present study describes the hormonal regulation on morphogenesis in vitro in nodal segments of T. indica. The nodal explants, sterilized with 0.1% HgCl2, were cultured on Murashige and Skoog Medium enriched with various combinations and concentrations of plant hormones auxin and cytokinin to study the hormonal regulation on morphogenesis in vitro in T. indica. BAP at high concentration could not evoke any morphogenetic response in nodal explants. Calli formation at the basal part of nodal explants were noted on medium containing BAP (0.1 mg/L) and 2,4-D (5.0 mg/L). 0.1 mg/L BAP was found most effective in the shoot development of the T. indica. Rhizogenesis was observed on half-strength MS medium supplemented with 1.0 mg/l IAA and 0.1 mg/l NAA. The study may also be used mass-propagation and conservation of this medicinal plant species.
Key-words- Plant growth regulators, Morphogenesis in vitro, Tylophora indica, Rhizogenesis
Influence of water stress and rhizobial inoculation on growth and yield of se...Innspub Net
Two season’s field experiment and single season screen house experiment were conducted to assess the effect of water stress periods and rhizobial inoculation in five P. vulgaris cultivars. The experiment consisted of two levels of rhizobia (with and without inoculation), two stress levels (with and without water stress) and five cultivars of P. vulgaris (KAT B9, KAT B1, F9 Kidney Selection, F8 Drought line and JESCA). Results showed that rhizobial inoculation significantly increased plant height (cm), leaf area (cm2), shoot and root dry weight (g-1 plant) and seed yields (kg-1 ha) at vegetative and flowering in field experiment. Furthermore, water stress treatments significantly reduced plant height (cm), stem diameter (mm), shoot and root dry weight (g-1 plant) and seed yields (kg-1 ha) in both growth stages at field experiment. For screen house experiment rhizobial inoculation significantly increased leaf area (cm2), number of leaves, stem girth (mm), shoot and root dry weight (g-1 plant) at both growth stages. Additionally, water stress treatments significantly reduced number of leaves, stem diameter (mm), shoot and root dry weight (g-1 plant) in both growth stages. Varieties F9 Kidney Selection, F8 Drought Line and JESCA had significantly superior measurements reflected in increased plant height (cm), shoot and root dry weight (g-1 plant) and seed yields (kg-1 ha) as compared with KAT B9 and KAT B1. Furthermore, significant interactive effects were also seen between rhizobial inoculation x stress level and tested bean cultivars on plant height, number of leaves, stem diameter, shoot dry weight and seed yields.
Radiosensitivity and Seedling Growth of Several Genotypes of Paddy Rice Mutan...AI Publications
Researchers use mutation induction in rice to create high genetic diversity. The basic population with high genetic diversity will facilitate the selection process for the desired good characters. This study aimed to determine the optimal dose that induces the highest genetic diversity in four lowland rice genotypes. The research materials were four genotypes of lowland rice, namely “G10”, “G16”, “Baas Selem”, and “Inpago Unram-1”. Gamma irradiation was carried out at the Center for Isotope and Radiation Application (PAIR) BATAN. Each genotype was irradiated at doses of 200, 300, 400 and 500 Gy. The seeding is done in the glasshouse of the Faculty of Agriculture, University of Mataram. Observations were made on the number of growing seeds, plant height and number of leaves. The LD50 value was determined based on the results of the regression analysis of the number of growing seeds at the four irradiation doses plus control (0 Gy). The results indicated that (1) the numbers of growing seeds decreased as the doses of gamma irradiation increased, (2) the LD50 value of the four rice genotypes ranged from 264 to 518 Gy, (3) the optimal dose of gamma ray irradiation for G10, G16, Baas Selem, and Inpago Unram-1 were 264 Gy, 398 Gy, 316 Gy and 518 Gy, respectively. (4) Among the four rice genotypes teasted, “G10” mutant was the most sensitive to gamma ray irradiation, whereas” Inpago Unram-1” mutant was the least sensitive genotype.
Use of nanofertilizers on fruit trees contributes effectively to improve the fruit quality and increasing the productivity of trees. It reduces environmental pollution by reducing the amount of fertilizers used, which is positively reflected in the increased economic return of the farmers. When nanofertilizers sprayed at very low concentration on fruit trees, these compounds have had a direct effect by increasing the growth, yield and quality of these fruit crops.
Influence of foliar fertilizing on stomata parameters in maize leaf (Zea mays...Innspub Net
In this research, the effects of foliar fertilizer Megegreen on stomata parameters of maize leaf (ZP 677) were studied. The experiment was performed on the experimental fields of the Institute of Agriculture, in Skopje, R. of Macedonia, during the 2008 and 2009. The foliar fertilizer was applied four times during the growing period in different concentrations of 0.3, 0.6 and 0.9% solution. Stomata density and size were measured on the adaxial and abaxial leaf surface from randomly selected plants from each replication in stage of silking. Stomata counts were made on the impressions from microscopic fields using the colodium method. Analyses of variance indicated that the application of foliar fertilizer has significant influence on stomata features on corn leaves. Results from research, show higher stomata density on adaxial (176,19-182,32 stomata /mm2) and abaxial surface (289,12-293,12 stomata/mm2) at variants 3 and 4. Variant 3 has the highest stomata length on adaxial surface (59,75 μm), without significant difference and the highest average length on the abaxial surface (63,00 μm), which is significantly different from the control variant. With the highest average width on adaxial leaf surface was variant 4 with 11,56 μm and on the abaxial surface was variant 2, with 13,49 μm. A positive significant correlation was observed between stomata number on the adaxial and abaxial surface of leaf (R2= 0,856**). Get the full articles at: http://www.innspub.net/volume-7-number-4-october-2015-ijaar/
Evaluating Kenyan Dolichos (Lablab purpureus L.) Genotypes for Resistance to ...Premier Publishers
The objective of this study was to evaluate eighteen Dolichos lablab genotypes for resistance to Maruca vitrata and Helicoverpa armigera in the field using morphological markers. The study design was Randomized Complete Block (RCBD) with separation of mean done using Turkey’s range of test. Eldoret, KALRO Njoro and KALRO Kakamega were the study sites. Morphological parameters of pods were studied to determine whether they have any influence on resistance of Dolichos lablab to M. vitrata and H. armigera. The pod damage (%) of each genotype was calculated and given a resistance rating of 1-5 score damage. Genotype G2, Bahati and W7 were resistant to M. vitrata in a scale of 1(0-10%; low infestation), Bahati and W7 were moderately resistant to H. armigera in a scale of 2(11-30%; moderate infestation). Genotype LG1MoiP10 was susceptible to M. vitrata in a scale of 4 (51-70%; severe infestation) and genotype M5 was intermediate to H. armigera in a scale of 3 (31-50%; high infestation). There was positive significant correlation in H. armigera and M. vitrata pod damage with days to maturity, growth habit, and pod attachment. Pod length and pod fragrance were positively correlated to M. vitrata. Negative correlation was detected in pod thickness, pod pubescence and raceme position to pod damage by H. armigera and M. vitrata. The study identified G2, Bahati and W7 as promising resistant genotypes and can be used in Dolichos breeding program. However, there is need to further evaluate them in different environments and seasons for reliability.
Growth analysis of rhizomania infected and healthy sugar beetJavad Rezaei
This study analyzed the growth of rhizomania-tolerant and susceptible sugar beet cultivars grown under infested and non-infested soil conditions. Results showed that under infested soil, susceptible cultivars produced 57% less root dry matter and 24% lower leaf area index than tolerant cultivars. Growth rates including crop growth rate and net assimilation rate were also lower for susceptible cultivars under infested conditions compared to tolerant cultivars. However, on non-infested soil, there were no significant differences in dry matter or growth indices between susceptible and tolerant cultivars. The study quantified the effects of rhizomania infection on sugar beet growth to provide data for modeling rhizomania impacts
This document discusses genetic diversity analysis of grape (Vitis vinifera L.) germplasm in India using microsatellite markers. A total of 42 grape genotypes were analyzed using 7 microsatellite markers. A total of 45 alleles were detected among the genotypes. The microsatellites grouped the genotypes into two main clusters (A and B) based on morphological and genetic characteristics, with subclusters differentiating seeded vs seedless fruits and pigmented vs non-pigmented fruits. The study found high genetic variability among the Indian grape germplasm and that microsatellite markers are a reliable tool for diversity and breeding programs.
Productivity of cassava sweet potato intercropping system as influenced by va...Alexander Decker
This study evaluated the productivity of a cassava-sweet potato intercropping system using different lengths of cassava cuttings in Makurdi, Nigeria over two growing seasons. Cassava cuttings of 20cm, 30cm, and 40cm lengths were intercropped with sweet potato. The highest yields for both crops were obtained when cassava cuttings were 30cm long, with land equivalent ratios of 2.15, indicating 53.5% higher land use efficiency than sole cropping. Intercropping cassava cuttings of 30cm length resulted in the greatest vegetative growth and highest yields for both crops, making it the most suitable combination for intercropping cassava and sweet potato.
Assessment of forage corn quality intercropping with green beans under influe...Innspub Net
To assess the quality of forage corn intercropping with green beans under the influence of Rhizobium bacteria and Arbuscular Mycorrhizal fungus, make a test in educational-research farm of agriculture faulty of Azna PNU that it was design in factorial to randomized complete block with three replications. The experimental factors include cropping systems such as mono cropping of corn, mono cropping of green beans, intercropping, Arbuscular Mycorrhizal fungus (use and non-use) and Rhizobium bacteria (use and non-use). The results showed that cropping systems on crude protein, wet forage weight, dry forage weight were significant at 1% level as well as leaf to stem ratio was significant at 5% level. Between different levels of bacteria used, acid detergent fiber was impressed and was significant at 5% level. Arbuscular Mycorrhizal fungus was significant at 5% level on water soluble carbohydrate. The results showed that the use of separate and combined of Rhizobium bacteria and Arbuscular Mycorrhizal fungus increase the quality of corn in intercropping than mono cropping. Finally with increasing of plant diversity and microorganism in soil increased the quality and quantity of forage. Get the full articles at: http://www.innspub.net/volume-6-number-5-may-2015-jbes/
No 12. impact of ga3 and naa on horticultural traits of abelmoschus esculentus.PARTNER, BADC, World Bank
This study investigated the effects of gibberellic acid (GA) and naphthalene acetic acid (NAA) on growth and yield of okra. GA and NAA were applied at 50 ppm to okra plants. GA treatment resulted in the tallest plants, most leaves, longest petioles, highest fresh and dry weights, earliest flowering, most flower buds and pods, and highest yield compared to NAA and control treatments. Specifically, GA treatment yielded 338.1 g per plant, 2.9 kg per plot, and 16.4 tons per hectare, outperforming the other treatments. The results suggest that GA has potential to increase growth and yield of okra more effectively than N
Comparison of Growth and Development of Pleurotus florida against Wastes from...Dr. siddhant
Two wastes from animal origin, viz., human hairs and egg shells were evaluated for different manifestations of white oyster mushroom, Pleurotus florida. The mushroom utilized both the
substrates for their growth and sporophore formation. The mycelial growth was significantly (P=0.05) faster on egg shell (18 days) as compared to human hair (23 days). The crop of mushroom was harvested in four flushes where human hairs showed higher yield and biological efficiency of mushroom (165 gm, 33%) than egg shells (155 gm, 31%), respectively. In respect of yield parameters such as yield, biological efficiency, number of mushroom fruit bodies and average weight of
sporophores, both the substrates were statistically at par to each other. Utilization of human hairs egg shells by P. florida reveals a new strategy for mycoremediation of these wastes.
Germination percentage and growing behavior of Salix tetrasperma (Willow) as ...Innspub Net
Propagation through branch cuttings is one of the best methods to produced tree nursery the yield of the tree produced from a cutting can be higher then a tree produced from seedlings, the conditions provided to them are important factor for getting good results. The aim of this study was to find the best size of cuttings for establishment of nursery and also to compare their performance in open air or in artificial conditions under plastic sheets cuttings of 2 inches, 4 and 6 of Salix tetrasperma were raised in plastic bags of size 3x7 their were three treatments with 25 bags in each and replicated 4 times. The data on sprouting percentage, plant height, root development etc was recorded after every two weeks. The data thus collected was analyzed statically using randomized complete block design. Result showed that cutting of 2 inches has high sprouting percentage and growth behavior as compared to other cuttings. Get more articles at: http://www.innspub.net/volume-6-number-4-april-2015-jbes/
Mutation Induction for Improvement of Banana (Musa Spp). "Berangan Cv. Intan-...paperpublications3
Abstract: In vitro mutation induction by using gamma ray at 20, 30, 40 and 60Gy was used to generate variability in triploid banana "Berangan cv. Intan (AAA), so as to provide the opportunity to select plants with desirable characters such as early fruiting and short stature. Mutation frequency increased with increased dosage whereas, survival and capacity to regenerate decreased with increased doses. Time to initiation varied from 4-8 weeks for gamma-irradiated materials compared to 2-3 weeks in the control. It appeared that the higher the dose, the longer it took for shoot initiation. The exposure of shoot-tip meristem pieces to radiation doses produced wide variation in growth and morphogenetic performance. Mutagenic treatments induced 2 to 3- fold increases in variability in both quantitative and qualitative traits at different stages, in vitro; at nursery and field. For the field-grown plants; the proportion varied from 2.9% for the control plants to 16.8% for 60 Gy and 20.1% for 40 Gy-treatments, while for treatments at 20 and 30 Gy variations was at 18% and 19.2%, respectively. The frequency of variants was highest in 40 Gy followed by 30 Gy and 20 Gy, while it was very low in 60 Gy except for plant stature (dwarfism or stunted growth). Earliness to flowering variants were recorded at 20, 30 and 40 Gy at low frequencies (0.6%, 0.7% and 1.7%) respectively, while none was observed for 60 Gy treatments. As in vitro mutation induction could create genetic variability as well as many undesirable variants, it is highly desirable to integrate in vitro mutation with a selection system that can screen for large mutagen treated population. The useful variants recorded for earliness to flowering were selected for 30 and 40 Gy treatments. 40 Gy showed high frequency in earliness as compared with 20 and 30 Gy., which came to flowering as early as 6 – 6.5 months compared to 7-8 months for control. The useful dwarf, which considered as desirable traits, showed a balance of height and girth.
Criteria of Most Efficient EcM Strains for Afforestation and Reforestation Pr...Muhammad Usman Mughal
This document discusses criteria for selecting the most efficient ectomycorrhizal (ECM) fungal strains for use in afforestation and reforestation programs. It defines afforestation and reforestation and introduces key selection criteria like compatibility with host plants, growth promotion effects, persistence in soil. Experimental methods are described for testing fungal isolates like glasshouse trials. A sample experiment shows Pisolithus tinctorius promoted growth in Eucalyptus species. Tables list efficient ECM fungi identified for various tree species. The use of ECM fungi in afforestation/reforestation programs and reasons for potential failures in field plantings are also summarized.
The effect of dosage of mycorrhizal Glomus mosseae and some varieties on grow...Innspub Net
This study aims to determine the effect of Glomus mosseae mycorrhizae dose and varieties on growth and yield of chili (Capsicum annum. L) on Entisol soil. This research was conducted at the UNSYIAH Experimental Garden, Banda Aceh. The design used in this study is factorial randomized block design (RBD). The first factor in this study is the dose of mycorrhizal Glomus mosseae (D) consisting of 4 levels, namely: D0= without mycorrhiza, D1= mycorrhiza Glomus mosseae 5g plant-1, D2= mycorrhiza Glomus mosseae 10g plant-1, and D3= mycorrhiza Glomus mosseae 15g plant-1. The second factor is chili variety (V) consisting of 3 levels, namely: PM 999 varieties, Lado F1 varieties, varieties CTH-01. The variables observed were growth parameters, chili yield, total N-content and P-available on Entisol soil and the percentage of mycorrhizal infected roots. The results showed that mycorrhizal doses were able to increase the value of N-total and P-available on the soil and can improve plant growth and yield, the use of mycorrhizal doses of 15 g tan-1 generally results in better growth and yield of chilli compared to other doses, while based on the results of research CTH-01 varieties affect the growth and yield of chili plants. There is an interaction between mycorrhizal doses with chilli varieties on growth parameters and chili yield.
High ambient temperature is the greatest problem faced by tropical greenhouse producing
vegetables. Temperature affects the productivity and growth of a plant, therefore using shades to alleviate this
effect could increase the yield of plants grown in greenhouses. This study intends to investigate the effect of
shading and greenhouse conditions on brinjal flowering and traits, and subsequently compare them to open
field cultivation of brinjal.
Evaluation of fungicides for their efficacy against seed-borne fungi of Groun...ICRISAT
Groundnut germplasm exchange has played a significant role in crop improvement programmes by providing wide genetic diversity world over. However, there is an inherent risk of introduction of new exotic pathogens or new/more virulent races of a pathogen into new areas. The basic principle of plant quarantine is to regulate the movement of seed material to mitigate the associated pest risk. Salvaging, a process of disinfecting infected seed, is an important quarantine strategy to prevent the introduction of new pathogens or virulent races into new areas.
In vitro STUDIES IN Baliospermummontanum (Willd) Mull. Arg.Midhun M Nair
This document summarizes an in vitro study of Baliospermum montanum, an endangered medicinal plant. Shoot tip cultures were used to regenerate plants, with the maximum elongation occurring in medium containing BA (5mg/l) and IBA (0.4mg/l). Nodal explants showed maximum axillary bud proliferation in medium with BA (4mg/l) and IAA (0.5mg/l). Stem explants produced the most callus when cultured in medium with BA (5mg/l) and 2,4-D (5mg/l). The study aims to develop tissue culture techniques to rapidly propagate B. montanum and protect it from overexploitation
The objectives of this study are: (i): To investigate and recognize the internal and abnormalities impacts induced by phytoplasma infection in the tomato host according to recent studies have shown that the association between plants and phytoplasmas can result in anatomical alteration in phloem tissues of infected plants, and great differences between healthy and diseased samples using microscopic examination of longitudinal, cross or ultra-thin sections of leaf blade, leaf petiole and stem. (ii): To determine the efficiency of different techniques toward production of phytoplasma-free tomato plantlets and mitigation of phytoplasma disease.
Mycorrhizal induced resistance and plant innate immunityOlivia Nongthombam
This document discusses mycorrhizal induced resistance (MIR) and plant innate immunity. It describes the different types of mycorrhizal associations including arbuscular mycorrhizae. MIR occurs in four phases and involves modulation of the plant immune system through changes in hormones like jasmonic acid and salicylic acid. Arbuscular mycorrhizal fungi can prime the jasmonic acid pathway in plants to induce a stronger defense response against pathogens. Gene expression is also altered in mycorrhizal plants to produce antifungal proteins that enhance resistance.
Antifungal activity of psoralea corylifolia hairy root extract against sugarc...researchagriculture
Red rot disease is the major constraint for sugarcane production in India and the pathogen has gained virulence in recent years. About 33 % reduction in yield was observed and loss in sucrose and commercial cane sugar was estimated upto 32 to 50 % in average infections. The present investigation was carried out in sugarcane breeding institute, Coimbatore to study the effect of Psoralea corylifolia hairy root extract against high intensity Colletotrichum falcatum spore suspension (106 spores ml-1) causing red rot disease reaction in canes under Controlled Condition Treatment (CCT) Chamber. Nodal infection, green top, internodal discoloration and internal discoloration of the canes in CCT chamber were taken as the parameters for fixing the disease evaluation after 10 days of incubation. The results of CCT method authenticated the results obtained under laboratory conditions. The study revealed 100 per cent effectiveness of two per cent P. corylifolia hairy root extract over red rot pathogen infection when compared to canes treated only with spore suspension of C. falcatum.
Article Citation:
Rajkumar D and Murugesan R.
Antifungal Activity of Psoralea corylifolia Hairy Root Extract against Sugarcane Red Rot Pathogen under Controlled Condition Treatment Chamber.
Journal of Research in Agriculture (2013) 2(2): 173-179.
Full Text:
http://www.jagri.info/documents/AG0047.pdf
Plants require essential nutrients for their growth and development that are mainly acquired from soil by their roots. Nutrient stress is an environmental condition that can seriously affect the production and quality of crop produce. Biofertilizers are the organisms (Bacteria, fungi, cyanobacteria, etc.) that enrich the nutrient quality of soil. Plants have a number of beneficial relationship with such organisms. Among these AM-Fungi are ubiquitous and form a mutuality relationship with roots of most plant species.
This document describes the development of a prototype pest management system using a wireless sensor network to monitor environmental parameters like temperature, humidity, and leaf wetness in apple and Kutki farms. The sensor data is transmitted wirelessly to a server to alert farmers when infection risk is high so they can take preventative measures and reduce unnecessary pesticide spraying. The system aims to improve crop growth and yield by monitoring conditions and notifying farmers to spray only when needed. The wireless sensor network allows for real-time monitoring across wide farm areas compared to traditional wired systems.
This document summarizes research on in vitro propagation of the medicinal plant Cocculus orbiculatus through cultivation from leaf explants. The study found that MS medium supplemented with 1.0 mg/L of BAP was optimal for inducing shoots directly from leaf explants. Combinations of BAP and adenine sulfate resulted in increased multiple shoot formation. The highest number of shoots was observed with 1.0 mg/L BAP and 5 mg/L adenine sulfate. The protocol established a reliable method for rapid multiple shoot induction and propagation of C. orbiculatus using leaf explants, cytokinins and adenine sulfate.
A SEMINAR REPORT ON POLLEN MICROBES BY TEMIDAYO FARORK OLAPADE.
Microorganisms including fungi, bacteria, and viruses live in flowers and are thought to affect pollination. Microbial influence the effectiveness of pollinator visits is poorly understood and depends on the context. The effect of microbes on pollen performance is underappreciated. Beyond the effect of pathogenic viruses, the impacts of pollen-transmitted endophytic microbes on pollen viability or tube growth are unknown but could affect the outcome of pollen receipt. Future research integrating microbes into pollination should broaden taxonomic diversity of microbes, pollinators and plants and the processes under study. Crops aimed at feeding an exponentially growing population are often exposed to a variety of harsh environmental factors. Although plants have evolved ways of adjusting their metabolism and some have also been engineered to tolerate stressful environments, there is still a shortage of food supply. An alternative approach is to explore the possibility of using rhizosphere microorganisms in the mitigation of abiotic stress and hopefully improve food production. Several studies have shown that rhizobacteria and mycorrhizae organisms can help improve stress tolerance by enhancing plant growth; stimulating the production of phytohormones, siderophores, and solubilizing phosphates; lowering ethylene levels; and upregulating the expression of dehydration response and antioxidant genes.
The Antibiotic and Antixenotic Resistance of Some Peanut (Arachis hypogea L.)...IJRES Journal
Resistant varieties is one of the important components in integrated pest management. Studies of the Antixenotic and antibiotic resistance of peanut (Arachis hypogea L.) varieties aims to obtain varieties resistant and suitable to be cultivated in the province of North Sulawesi. Research using factorial experiment, treatment peanut varieties: Local varieties (V1), Giraffe (V2), Bison (V3), Bima (V4), Elephant (V5) and Rabbit (V6). Organic fertilizer treatments: Without fertilization (P0) and Super Petroganik as much as 20 tonnes/ha (P1) with three replications. Application of organic fertilizer made one month before planting seeds. Antixenosis and antibiosis resistance research results at 30 days after planting (DAP), the highest stomata diameter contained in P1V1 treatment is 12.62 μm, the longest trichomes on P0V3 is 88.07 μm, the highest stem cotrtex on P1V2 is 88.30 μm, and the highest contained on P1V6 is 9.96 mg/g. The result of the 60 DAP is obtained that the highest yields diameter stomata, trichomes length and content of flavonoids in P1V6 each is 13.85 μm, 136.33 μm and 14:57 mg/g, being the highest cortex diameter on P1V5 is 158.80 μm. Based on the results of analysis of variance, flavonoid content peanut varieties 30 DAP and 60 DAP significantly different (P < 0.05), while the diameter of stomata, trichomes numbers and length number, and corticial stem not significant (P > 0.05). Resistance mechanisms developed peanut plants are antibiosis.
1. The document summarizes the pros and cons of using silver nanoparticles against agriculturally important microbes as presented by Surender Kumar.
2. The pros discussed include the broad spectrum antimicrobial activity of silver nanoparticles, their ability to be synthesized through green methods, effectiveness at low concentrations, and reduced likelihood of microbial resistance developing.
3. The cons examined are the potential harmful effects on beneficial microbes, environmental impacts, toxicity risks to plants, development of microbial resistance over time, and human health concerns. The presentation concludes by emphasizing the need for further research and regulatory guidelines to ensure the safe use of silver nanoparticles in agriculture.
Mutation Induction for Improvement of Banana (Musa Spp.) Berangan Cv. Intan-AAApaperpublications3
Abstract: In vitro mutation induction by using gamma ray at 20, 30, 40 and 60Gy was used to generate variability in triploid banana "Berangan cv. Intan (AAA), so as to provide the opportunity to select plants with desirable characters such as early fruiting and short stature. Mutation frequency increased with increased dosage whereas, survival and capacity to regenerate decreased with increased doses. Time to initiation varied from 4-8 weeks for gamma-irradiated materials compared to 2-3 weeks in the control. It appeared that the higher the dose, the longer it took for shoot initiation. The exposure of shoot-tip meristem pieces to radiation doses produced wide variation in growth and morphogenetic performance. Mutagenic treatments induced 2 to 3- fold increases in variability in both quantitative and qualitative traits at different stages, in vitro; at nursery and field. For the field-grown plants; the proportion varied from 2.9% for the control plants to 16.8% for 60 Gy and 20.1% for 40 Gy-treatments, while for treatments at 20 and 30 Gy variations was at 18% and 19.2%, respectively. The frequency of variants was highest in 40 Gy followed by 30 Gy and 20 Gy, while it was very low in 60 Gy except for plant stature (dwarfism or stunted growth). Earliness to flowering variants were recorded at 20, 30 and 40 Gy at low frequencies (0.6%, 0.7% and 1.7%) respectively, while none was observed for 60 Gy treatments. As in vitro mutation induction could create genetic variability as well as many undesirable variants, it is highly desirable to integrate in vitro mutation with a selection system that can screen for large mutagen treated population. The useful variants recorded for earliness to flowering were selected for 30 and 40 Gy treatments. 40 Gy showed high frequency in earliness as compared with 20 and 30 Gy., which came to flowering as early as 6 – 6.5 months compared to 7-8 months for control. The useful dwarf, which considered as desirable traits, showed a balance of height and girth.
The use of plants extracts in the improvement of cowpea yield at dang (Ngaoun...Innspub Net
In the substitution of chemical insecticides with potential biopesticides, the efficiency of Lippia multiflora, Plectranthus glandulosus and Callistemon rigidus were evaluated on the improvement of cowpea (Vinia unguiculata) in Dang (Ngaoundere, Cameroon). The experiment was conducted in a completely randomized block design with 5 treatments repeated 4 times each: the negative control, the positive control (Decis), L. multiflora, P. glandulosus and C. rigidus. These different insecticides were sprayed on cowpea plants with 14 days interval starting from the 14th day after sowing. The parameters collected were the diversity of insect pests, the number of pods, and the dry weight of the grains. All of the insecticides used improved cowpea yields. Although less efficient than Decis, most biopesticides significantly (p < 0.001) improved the yield of cowpeas compared to the negative control. This improvement was 260% for C. rigidus, and 120% for P. glandulosus. These results suggest that C. rigidus, P. glandulosus and L. multiflora could be considered as potential substitutes for chemical insecticides in improving cowpea yields.
The use of plants extracts in the improvement of cowpea yield at dang (Ngaoun...Innspub Net
In the substitution of chemical insecticides with potential biopesticides, the efficiency of Lippia multiflora, Plectranthus glandulosus and Callistemon rigidus were evaluated on the improvement of cowpea (Vinia unguiculata) in Dang (Ngaoundere, Cameroon). The experiment was conducted in a completely randomized block design with 5 treatments repeated 4 times each: the negative control, the positive control (Decis), L. multiflora, P. glandulosus and C. rigidus. These different insecticides were sprayed on cowpea plants with 14 days interval starting from the 14th day after sowing. The parameters collected were the diversity of insect pests, the number of pods, and the dry weight of the grains. All of the insecticides used improved cowpea yields. Although less efficient than Decis, most biopesticides significantly (p < 0.001) improved the yield of cowpeas compared to the negative control. This improvement was 260% for C. rigidus, and 120% for P. glandulosus. These results suggest that C. rigidus, P. glandulosus and L. multiflora could be considered as potential substitutes for chemical insecticides in improving cowpea yields.
Effects of a Composite Endomycorrhizal Inoculum on Olive Cuttings under the G...IJEAB
This study was carried out in a nursery to evaluate the impact of mycorrhizal fungi on the cutting’s root growth, and root colonization of a Moroccan olive variety ‘Picholine Marocaine’ under greenhouse conditions during 2 years of cultivation. The results revealed that the inoculation with a composite inoculum of arbuscular mycorrhizal fungi (AMF) stimulated an early root formation and high development of vegetative shoots in inoculated cuttings respectively, 35 days (50 days in the control plots) and 40 days (60 days in the control plots) after their culture. The progressive establishment of mycorrhizal symbiosis in the roots of the inoculated plants showed that the root and vegetative masses were respectively 24 g and 19.5 g two years after inoculation. The average height and the leave’s number of the inoculated plants relative to the control were respectively s 42/ 12 cm and 145/12. The newly formed roots were mycorrhizal and present different structures characteristic of AMF: arbuscules, vesicles, hyphae and spores, whose frequency and intensity reached 90% and 75% two years after cuttings cultivation. The arbuscular and vesicular contents and the number of spores were 67%, 96% and 212 spores/ 100 g of soil respectively. The fourteen species of mycorrhizal fungi isolated from the rhizosphere belong to 4 genera (Glomus, Acaulospora, Gigaspora, and Scutellospora) and three families (Glomaceae, Acaulosporaceae and Gigasporacea).The Glomus genus was the most dominant (65%) followed by the Gigaspora genus (22%). Glomus intraradices, Gigaspora sp.2, Glomus versiformes are the most abundant species, their frequency of occurrence are respectively 30%, 21% and 16%.
IJRET : International Journal of Research in Engineering and Technology is an international peer reviewed, online journal published by eSAT Publishing House for the enhancement of research in various disciplines of Engineering and Technology. The aim and scope of the journal is to provide an academic medium and an important reference for the advancement and dissemination of research results that support high-level learning, teaching and research in the fields of Engineering and Technology. We bring together Scientists, Academician, Field Engineers, Scholars and Students of related fields of Engineering and Technology
This document proposes a study on factors of resistance in maize plants against the spotted stem borer, Chilo partellus. The study will assess molecular diversity and biochemical/nutrient levels in maize cultivars to establish correlations with borer infestation levels. It will also examine physical resistance factors like plant architecture, stem diameter, and leaf trichome density. The research will involve growing 26 maize genotypes and quantifying borer damage, molecular profiles, nutrient levels, and physical traits to identify sources of resistance.
The Production of Triploid Clariobranchus in Indoor HatcheryIOSR Journals
This study evaluated the interactive effects of rhizobium and virus inocula on three cowpea cultivars. The cultivars were inoculated with two rhizobium strains (R25B and IRj2180A) and two virus strains (CABMV and CYMV) at two different times. Viral inoculation significantly reduced nodulation, biomass production, and grain yields across all cultivars. Maximum reductions occurred without rhizobium inoculation. Early inoculation had a greater effect than late inoculation. The interaction of rhizobium and virus strains showed that viral severity was not reduced by rhizobium presence. Cultivar IT90K-277-2 performed best
The effects of explant rotation, medium types, JA and GA3 additions on in vit...Agriculture Journal IJOEAR
— This study investigates the effects on the in vitro microtuber formations of Solanum tuberosum L. cv. Marfona species, effected by the direction of planting the explants (horizontal or vertical), the type of medium (solid or two-phase), adding Jasmonic acid (JA) (0.0, 10 ng/L, 1 µg/L and 0.2 mg/L) and Gibberellic acid (GA 3) (0.0 and 0.2 mg/L). The cultures were incubated in a climate chamber at 22-25 o C, and were subjected to a light intensity of 145 µmol m-2 s-1 for 8 hours in light and 16 hours in dark photoperiods (short day) for 4-6 weeks. Microtuber production was inhibited when GA 3 was added. The maximum number of microtubers was observed when the explants were planted vertically and were grown in two-phase medium which did not contain GA 3 and had 10 ng/L JA present. It was determined that two-phase medium with 0.2 mg/L JA but without GA 3 was the most favourable medium for tuber growth (for both height and width). The best microtuber formation on single node explants were observed to occur in the short day photoperiod (8 hours light/16 hours dark) in a two-phase medium that contained 0.2 mg/L JA without the addition of GA 3. The results shows that the effect caused by JA works antagonistically with that of GA 3 thus causing the resulting microtuber formation observed.
Criteria for the Selection of Vegetable Growth-Promoting Bacteria to be appli...Agriculture Journal IJOEAR
In order to define which are the most important criteria for the selection of plant Growth-Promoting bacterial strains of the Hibiscus sabdariffa L. crop (Roselle), bacterial strains isolated from the roots of Roselle plants of two varieties (Creole and Spider) were used, collected in the community of Río de los Peces, municipality of Candelaria Loxicha, Oaxaca and seeds of the same varieties. To characterize the varieties, the following were determined: total germination percentage (TGP), germination speed (GS), the root length(RL), the stem length (SL), the dry root biomass (DRB), the dry stem biomass (DSB) and the chlorophyll content (CC). Three types of LED lamps were used to illuminate the seedlings. The seeds inoculated with cells of six selected bacterial strains were grown in a greenhouse to determine: the stem length (SL) at 3, 45 and 65 days after sowing (das). The treatments were distributed under a completely random design and comparison of means (Tukey, p = 0.05). The TGP, DSB and DRB parameters were not useful in the selection process of the strains that promoted plant growth to a greater degree. The GS and SL to be considered safe criteria or not, what is important is the relationship of what happens at the time of germination and development of the seedlings in the laboratory and greenhouse. The SL of the plants in the greenhouse showed differences between strains, but not regarding the control and also only observed in the first days of development (3 das). The CC did not prove to be a good selection criterion either. The lamp composed of 15% white light, 27% blue light and 58% red light was the one that most promoted root growth.
Micropropagation of Santalum Album L. Sandalwoodijtsrd
An efficient plant regeneration protocol was developed for Santalum album L. Santalaceae , an economically important species. Plant regeneration was achieved using nodal explants and leaf disc on Murashige and Skoog MS medium for direct shoot regeneration. Effect of Plant Growth Regulators PGR like 6 Benzyl Adenine BA , Kinetin KN and 2 Isopentenyl adenine 2 iP on shoot initiation 2 Isopentenyl adenine and Gibberellic acid GA3 for shoot elongation and multiple shoot formation and Indole 3 Butyric Acid IBA and a Naphthalene Acetic Acid NAA for rooting was studied. Among the explants tested for shoot induction, nodal segments proved good results. The best treatment for obtaining shoot induction was 3.0mg L BAP and for rooting 1 mg L of IBA was found to be the best treatment combination for maximum sprouting of shoot and rooting. After six the rooted plantlets were transferred for hardening, 20 of plantlets survived and resumed growth in the mixture of soil, vermiculite and sand 1 1 1 . S. Aghi Zion Inbakani | S. Sathishkumar | Bakan Jagdish Sudhakar "Micropropagation of Santalum Album L. (Sandalwood)" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-4 , June 2021, URL: https://www.ijtsrd.compapers/ijtsrd43698.pdf Paper URL: https://www.ijtsrd.combiological-science/biotechnology/43698/micropropagation-of-santalum-album-l-sandalwood/s-aghi-zion-inbakani
Isolation of endophytes from potato and their antagonist effect against Fusar...Innspub Net
Plant endophytes may be intercellular or intracellular depending upon their location in the plant tissue because they are present inside the cells or in the intracellular space, respectively. Isolation of endophytic bacteria has been reported from both monocot and dicot plants, ranging from woody trees, such as teak and pear, to herbaceous crop plants such as mustard and maize. The aim of this study was the isolation of endophytes from potato and their antagonist effect against Fusarium oxysporum. Endophytic fungi were isolated from leaves, stems and roots of healthy Potato plant derived from Chak No.359/E.B Village, Tehsil Burewala. Isolation of endophytic fungi from plant parts was done according to the method described by Petrini. The media used in the present study was the Potatodextrose agar (PDA) for fungus and nutrient agar medium for maintaining bacterial stains. F.oxysporum was taken from the Plant pathology lab of UAF sub-campus Burewala-Vehari . The results of the experiment clearly revealed that the stems, root and leaf of the potato plants under present investigation had the maximum colonization frequency for fungal endophytes. Fusarium oxysporum showed rapid growth 5-7cm in5 days. Fusarium oxysporum was white and growing rapidly that later produced dark violet pigments in PDA. Erwinia showed light green, circular, shining, slimy, smooth characteristics. The isolate strain of Bacillus showed rodshaped, fuzzy white or slightly yellow circular and irregular characteristics.
Similar to Effect of Clariodeoglomusclariodeorum on morphology and abundant of carrot root hairs in vitro (20)
The Statutory Interpretation of Renewable Energy Based on Syllogism of Britis...AI Publications
The current production for energy consumption generates harmful impacts of carbon dioxide to the environment causing instability to sustainable development goals. The constitutional reforms of British Government serve to be an important means of resolving any encountered incompatibilities to political environment. This study aims to evaluate green economy using developed equation for renewable energy towards political polarization of corporate governance. The Kano Model Assessment is used to measure the equivalency of 1970 Patents Act to UK Intellectual Property tabulating the criteria for the fulfillment of sustainable development goals in respect to the environment, artificial intelligence, and dynamic dichotomy of administrative agencies and presidential restriction, as statutory interpretation development to renewable energy. The constitutional forms of British government satisfy the sustainable development goals needed to fight climate change, advocate healthy ecosystem, promote leadership of magnates, and delegate responsibilities towards green economy. The presidential partisanship must be observed to delineate parties of concerns and execute the government prescriptions in equivalence to the dichotomous relationship of technology and the environment in fulfilling the rights and privileges of all citizens. Hence, the political elites can execute corporate governance towards sustainable development of renewable energy promoting environmental parks and zero emission target of carbon dioxide discharges. The economic theory developed in statutory interpretation for renewable energy serves as a tool to reduce detrimental impacts of carbon dioxide to the environment, mitigate climate change, and produce artefacts of bioenergy and artificial intelligence promoting sustainable development. It is suggested to explore other vulnerabilities of artificial intelligence to prosper economic success.
Enhancement of Aqueous Solubility of Piroxicam Using Solvent Deposition SystemAI Publications
Piroxicam is a non-steroidal anti-inflammatory drug that is characterized by low solubility-high permeability. The present study was designed to improve the dissolution rate of piroxicam at the physiological pH's through its increased solubility by using solvent deposition system.
Analysis of Value Chain of Cow Milk: The Case of Itang Special Woreda, Gambel...AI Publications
Ethiopia has a long and rich history of dairy farming, which was mostly carried out by small and marginal farmers who raised cattle, camels, goats, and sheep, among other species, for milk. Finding the Itang Special Woreda cow milk value chain is the study's main goal. In order to gather primary data, 204 smallholder dairy farmer households were randomly selected, and the market concentration ratio was calculated using 20 traders. Descriptive statistics, econometric models, and rank analysis were used to achieve the above specified goals. Out of all the participants in the milk value chain, producers, cafés, hotels, and dairy cooperatives had the largest gross marketing margins, accounting for 100% of the consumer price in channels I and II, 55% in channels III and V, and 25.5% in channels V. The number of children under five, the number of milking cows owned, the amount of money from non-dairy sources, the frequency of extension service contacts, the amount of milk produced each day, and the availability of market information were found to have an impact on smallholders' involvement in the milk market. Numerous obstacles also limited the amount of milk produced and marketed. The poll claims that general health issues, sickness, predators, and a lack of veterinary care are plaguing farmers. In order to address the issue of milk perishability, the researchers recommended the host community and organization to construct an agro milk processor, renovate the dairy cooperative in the study region, and restructure the current conventional marketing to lower the transaction and cost of milk marketing.
Minds and Machines: Impact of Emotional Intelligence on Investment Decisions ...AI Publications
In the evolving landscape of financial decision-making, this study delves into the intricate relationships among Emotional Intelligence (EI), Artificial Intelligence (AI), and Investment Decisions (ID). By scrutinizing the direct influence of human emotional intelligence on investment choices and elucidating the mediating role of AI in this process, our research seeks to unravel the complex interplay between minds and machines. Through empirical analysis, we reveal that EI not only directly impacts ID but also exerts its influence indirectly through AI-mediated pathways. The findings underscore the pivotal role of emotional awareness in investor decision-making, augmented by the technological capabilities of AI. It suggests that most investors are influenced by the identified emotional intelligence when making investment decisions. Furthermore, AI substantially impacts investors' decision-making process when it comes to investing; nevertheless, AI partially mediates the relationship between emotional intelligence and investment decisions. This nuanced understanding provides valuable insights for financial practitioners, policymakers, and researchers, emphasizing the need for holistic strategies that integrate emotional and technological dimensions in navigating the intricacies of modern investment landscapes. As the synergy between human intuition and artificial intelligence becomes increasingly integral to financial decision-making, this study contributes to the ongoing discourse on the symbiotic relationship between minds and machines in investments.0
Bronchopulmonary cancers are common cancers with a poor prognosis. It is the leading cause of death by cancer in Algeria and in the world. Behind this unfavorable prognosis hides numerous disparities according to age, sex, and exposure to risk factors, ranking 4th among incident cancers and developing countries including Algeria, all sexes combined. It ranks 2nd cancers in men and 3rd among women. Whatever the age observed, the incidence of this cancer is higher in men than in women, however the gap is narrowing to the detriment of the latter. The results of scientific research agree to relate trends in incidence and mortality rates to tobacco consumption, including passive smoking. Furthermore, other risk factors are mentioned such as exposure to asbestos in the workplace or to radon for the general population, or even genetic predisposition. However, the weight of these etiological and/or predisposing factors is in no way comparable to that of tobacco in the genesis of lung cancer and the resulting mortality. We provide a literature review in our article on the descriptive and analytical epidemiology of lung cancer.
Further analysis on Organic agriculture and organic farming in case of Thaila...AI Publications
The objective of this paper is to present Further analysis on Organic agriculture and organic farming in case of Thailand agriculture and enhancing farmer productivity. In view of the demand for organic fertilizers, efforts should also be made to enhance and to develop more effective of compost, bio-fertilizer, and bio-pesticides currently used by farmers. Likewise, emphasis should also be laid on the cultivation of legumes and other crops that can enhance the fertility of the soil, as practiced by farmers in many developing countries to fertilize their lands. On the other hand, most of the farmers who practice this farm system found that they are adopting a number of SLMs and interested in joining the meeting or training to gain more and more knowledge.
Current Changes in the Role of Agriculture and Agri-Farming Structures in Tha...AI Publications
The objective os this study is to present Current Changes in the Role of Agriculture and Agri-Farming Structures in Thailand and Vietnam with SLM practices. Farmer’s adoption and investment in SLM is a key for controlling land degradation, enhancing the well-being of society, and ensuring the optimal use of land resources for the benefit of present and future generations (World Bank, 2006; FAO, 2018). And agriculture remains an essential element of lives of many farmers in term of the strong cultural and symbolic values that attach current working generation to do and to spend time for it but not intern of income generating.
Growth, Yield and Economic Advantage of Onion (Allium cepa L.) Varieties in R...AI Publications
Haphazard and low soil fertility, low yielding verities and poor agronomic practices are among the major factors constraining onion production in the central rift valley of Ethiopia. Therefore, a field experiment was conducted in East Showa Zone of Adami Tulu Jido Combolcha district in central rift valley areas at ziway from October 2021 to April 2022 to identify appropriate rate of NPSB fertilizer and planting pattern of onion varieties. The experiment was laid out in split plot design of factorial arrangement in three replications. The main effect of NPSB blended fertilizer rates and varieties (red coach and red king) significantly (p<0.01) influenced plant height, leaf length, leaf diameter, leaf number and fresh leaf weight, shoot dry matter per plant, and harvest index. Total dry biomass, bulb diameter, neck diameter, average fresh bulb weight, bulb dry matter, marketable bulb yield, and total bulb yield were significantly (p<0.01) influenced only by the main effect of NPSB blended fertilizer rates. In addition, unmarketable bulb yield was statistically significantly affected (p≥0.05) by the blended fertilizer rates and planting pattern. Moreover, days to 90% maturity of onion was affected by the main factor of NPSB fertilizer rate, variety and planting pattern. The non-fertilized plants in the control treatment were inferior in all parameters except unmarketable bulb yield and harvest index. Significantly higher marketable bulb yield (41 t ha-1) and total bulb yield (41.33 t ha-1) was recorded from 300 kg ha-1 NPSB blended fertilizer rate applied. Double row planting method and hybrid red coach onion variety had also gave higher growth and yields. The study revealed that the highest net benefit of Birr, 878,894 with lest cost of Birr 148,006 by the combinations of 150 kg blended NPSB ha-1 with double row planting method (40cm*20cm*7cm) and red coach variety which can be recommendable for higher marketable bulb yield and economic return of hybrid onion for small scale farmers in the study area. Also, for resource full producers (investors), highest net benefit of Birr 1,205,372 with higher cost (159,628 Birr) by application of 300 kg NPSB ha-1 is recommended as a second option. However, the research should be replicated both in season and areas to more verify the recommendations.
Evaluation of In-vitro neuroprotective effect of Ethanolic extract of Canariu...AI Publications
The ethanolic extract of canarium solomonense leaves (ecsl) was studied for its neuroprotective activity. The neuroprotective activity of ECSL was found to have a significant impact on neuronal cell death triggered by hydrogen peroxide (MTT assay) in human SH-SY5Y neuroblastoma cells. Scopolamine, a muscarinic receptor blocker, is frequently used to induce cognitive impairment in laboratory animals. Injections of scopolamine influence multiple cognitive functions, including motor function, short-term memory, and attention. Using the Morris water maze, the Y maze, and the passive avoidance paradigm, memory enhancing activity in scopolamine-induced amnesic rats was evaluated. Using the Morris water maze, the Y maze, and the passive avoidance paradigm, ECSL was found to have a substantial effect on the memory of scopolamine- induced amnesic rats. Our experimental data indicated that ECSL can reverse scopolamine induced amnesia and assist with memory issues.
The goal of neuroprotection is to shield neurons against damage, whether that damage is caused by environmental factors, pathogens, or neurodegenerative illnesses. Inhibiting protein-based deposit buildup, oxidative stress, and neuroinflammation, as well as rectifying abnormalities of neurotransmitters like dopamine and acetylcholine, are some of the ways in which medicinal herbs have neuroprotective effects [1-3]. This review will focus on the ways in which medicinal herbs may protect neurons.
A phytochemical and pharmacological review on canarium solomonenseAI Publications
The genus Canarium L. consists of 75 species of aromatic trees which are found in the rainforests of tropical Asia, Africa and the Pacific. The medicinal uses, botany, chemical constituents and pharmacological activities are now reviewed. Various compounds are tabulated according to their classes their structures are given. Traditionally canarium solomonense have been used to treat a broad array of illnesses. Pharmacological actions for canarium solomonense as discussed in this review include antibacterial, antimicrobial, antioxidant, anti-inflammatory, hepatoprotective and antitumor activity.
Influences of Digital Marketing in the Buying Decisions of College Students i...AI Publications
This research investigates the influence of digital marketing channels on purchasing decisions among college students in Ramanathapuram District. The study highlights that social media marketing, online advertising, and mobile marketing exhibit substantial positive effects on purchase decisions. However, email marketing's impact appears to be more complex. Moreover, the study explores how demographic variables like gender and academic level shape these effects. Notably, freshman students display varying susceptibility to specific digital marketing messages compared to their junior, senior, or graduate counterparts. These findings offer crucial insights for marketers aiming to tailor their strategies effectively to the preferences and behaviors of college students. By understanding the differential impacts of various digital marketing channels and considering demographic nuances, marketers can refine their approaches, optimize engagement, and ultimately enhance the effectiveness of their campaigns in targeting this demographic.
A Study on Performance of the Karnataka State Cooperative Agriculture & Rural...AI Publications
The Karnataka State Co-operative Agriculture and Rural Development Bank Limited is the apex bank of all the primary co-operative agriculture and rural development banks in the state. All the PCARD Banks in the state are affiliated to it. The KSCARD Bank provides financial accommodation to the PCARD Banks for their lending operations. In order to quick sanction and disbursement of loans and supervision over the PCARD Banks the KSCARD Bank has opened district level branches. Bank has established Women Development Cell to promote entrepreneurship among women in 2005. The Bank is identifying women borrowers in the rural areas by assigning suitable projects to motivate their self-confidence to lead independent life. Progress made in financing women entrepreneurs women.
Breast hamartoma is a rare, well-circumscribed, benign lesion made up of a variable quantity of glandular, adipose and fibrous tissue. This is a lesion that can affect women at any age from puberty. With the increasingly frequent use of imaging methods such as mammography and ultrasound as well as breast biopsy, cases of hamartoma diagnosed are increasing. The diagnosis of these lesions is made by mammography. The histological and radiological aspects are variable and depend on its adipose tissue content. The identification of these lesions is important in order to avoid surgical excisions. We report radio-clinical and pathological records of breast hamartoma.
A retrospective study on ovarian cancer with a median follow-up of 36 months ...AI Publications
Ovarian cancer is relatively common but serious and has a poor prognosis. The aim of this study is to highlight the epidemiological, diagnostic, therapeutic and evolutionary aspects of this malignant pathology managed at the Bejaia university hospital center. This is a retrospective and descriptive study over a period of 3 years (2019 - 2022) carried out on 20 patients who developed ovarian cancer. The average age of the patients was 50 years old, 53.23% of whom were over 45 years old. The CA-125 blood test was positive in 18 out of 20 patients. The tumors were discovered on ultrasound in 87.10% of cases and at laparotomy in 12.90%. Total hysterectomy with bilateral adnexectomy was the most performed procedure (64.52%). The early postoperative course was simple. 15 patients underwent second look surgery (16.13%) for locoregional recurrences. Epithelial tumors were the most frequent histological type (93.55%), including 79% in the advanced stage ( IIIc -IV) and 21% in the early stage (Ia- Ib ). Adjuvant chemotherapy was administered in 80% of patients. With a median follow-up of 36 months, 2 patients were lost to follow-up. The evolution was favorable in 27.42% and in 25.81% deaths occurred late postoperatively. Ovarian cancer is not common but serious given the advanced stages and the high rate of late postoperative deaths which were largely observed in patients deprived of adequate neoadjuvant or adjuvant chemotherapy.
More analysis on environment protection and sustainable agriculture - A case ...AI Publications
This study presents a case of tea and coffee crops , esp. environment protection and sustainable agriculture in Son La and Thai Nguyen of Vietnam. Research results show us that The process of having an agricultural product goes through many steps such as planting, planning, harvesting, packing, transporting, storing and distributing. - The State adopts policies to encourage innovation of agricultural production models and methods towards sustainability, adapting to climate change, saving water, and limiting the use of inorganic fertilizers and pesticides. chemicals and products for environmental treatment in agriculture; develop environmentally friendly agricultural models. Our research limitation is that we can expand for other crops, industries and markets as well.
Assessment of Growth and Yield Performance of Twelve Different Rice Varieties...AI Publications
The present investigation entitled “Assessment of growth and yield performance of twelve different rice varieties under north Konkan coastal zone of Maharashtra” was carried out during the kharif season of the year 2021 and 2022 on the field of ASPEE, Agricultural Research and Development Foundation, Tansa Farm, At Nare, Taluka Wada, District Palghar, Maharashtra, India. The experiment was laid out in Randomized Block Design (RBD). The twelve varieties namely Zini, Jaya, Dandi, Rahghudya, Govindbhog, Dangi, Gurjari, VNR-7, VNR-8, VNR-9, Karjat-3, and Karjat-5 were replicated thrice. The plant height (cm), number of tillers per plant, number of panicles per plant, number of panicles (m²), and length of panicle (cm) were noted to the maximum with cv. “VNR-7”. The highest number of seeds per panicle, test weight (gm), grain yield (q/ha), and straw yield (q/ha) were recorded with the cv. “VNR-7”. While the lowest number of days to 50% flowering was also recorded with cv. “VNR-7” during the year 2021 and 2022.
Cultivating Proactive Cybersecurity Culture among IT Professional to Combat E...AI Publications
In the current digital landscape, cybercriminals continually evolve their techniques to execute successful attacks on businesses, thus posing a great challenge to information technology (IT) professionals. While traditional cybersecurity approaches like layered defense and reactive security have helped IT professionals cope with traditional threats, they are ineffective in dealing with evolving cyberattacks. This paper focuses on the need for a proactive cybersecurity culture among IT professionals to enable them combat evolving threats. The paper emphasis that building a proactive security approach and culture can help among IT professionals anticipate, identify, and mitigate latent threats prior to them exploiting existing vulnerabilities. This paper also points out that as IT professionals use reactive security when dealing with traditional attacks, they can use it collaboratively with proactive security to effectively protect their networks, data, and systems and avoid heavy costs of dealing with cyberattack’s aftermaths and business recovery.
The Impacts of Viral Hepatitis on Liver Enzymes and BilrubinAI Publications
Viral hepatitis is an infection that causes liver inflammation and damage. Several different viruses cause hepatitis, including hepatitis A, B, C, D, and E. The hepatitis A and E viruses typically cause acute infections. The hepatitis B, C, and D viruses can cause acute and chronic infections. Hepatitis A causes only acute infection and typically gets better without treatment after a few weeks. The hepatitis A virus spreads through contact with an infected person’s stool. Protection by getting the hepatitis A vaccine. Hepatitis E is typically an acute infection that gets better without treatment after several weeks. Some types of hepatitis E virus are spread by drinking water contaminated by an infected person’s stool. Other types are spread by eating undercooked pork or wild game. Hepatitis B can cause acute or chronic infection. Recommendation for screening for hepatitis B in pregnant women or in those with a high chance of being infected. Protection from hepatitis B by getting the hepatitis B vaccine. Hepatitis C can cause acute or chronic infection. Doctors usually recommend one-time screening of all adults ages 18 to 79 for hepatitis C. Early diagnosis and treatment can prevent liver damage. The hepatitis D virus is unusual because it can only infect those who have a hepatitis B virus infection. A coinfection occurs when both hepatitis D and hepatitis B infections at the same time. A superinfection occurs already have chronic hepatitis B and then become infected with hepatitis D. The aim of this study is to find the effect of each type of viral hepatitis on the bilirubin (TB , DSB) , and liver enzymes; AST, ALT, ALP,GGT among viral hepatitis patients. 200 patients were selected from the viral hepatitis units in the central public health laboratory in Baghdad city, all the chosen cases were confirmed as a positive samples , they are classified into four equal group each with fifty individual and with a single serological viral hepatitis type either; anti-HAV( IgM ) , HBs Ag , anti-HCV ,or anti-HEV(IgM ). All patients were tested for; serum bilirubin ( TB ,D.SB ) , AST , ALT , ALP , GGT. Another fifty quite healthy and normal person was selected as a control group for comparison. . Liver enzymes and bilirubin changes are more pronounced in HAV, HEV than HCV and HBVAST and ALT lack some sensitivity in detecting HCV ,HBV and mild elevations of ALT or AST in asymptomatic patients can be evaluated efficiently by considering ,hepatitis B, hepatitis C. ALT is generally a more sensitive indicator of acute liver cell damage than AST, It is relatively specific for hepatocyte necrosis with a marked elevations in viral hepatitis. Liver enzymes and bilirubin changes are more pronounced in HAV, HEV than HCV and HBV.AST and ALT lack some sensitivity in detecting HCV ,HBV and mild elevations of ALT or AST in asymptomatic patients can be evaluated efficiently by considering ,hepatitis B, hepatitis C. ALT is generally a more sensitive indicator of acute liver
Determinants of Women Empowerment in Bishoftu Town; Oromia Regional State of ...AI Publications
The purpose of this study was to determine the status of women's empowerment and its determinants using women's asset endowment and decision-making potential as indicators. To determine representative sample size, this study used a two-stage sampling technique, and 122 sample respondents were selected at random. To analyze the data in this study, descriptive statistics and a probit model were used. The average women's empowerment index was 0.41, indicating a relatively lower status of women's empowerment in the study area. According to the study's findings, only 40.9% of women were empowered, while the remaining 59.1% were not. The probit model results show that women's access to the media, women's income, and their husbands' education status have a significant and positive impact on the status of women's empowerment, while the family size of households has a negative impact. As a result, it is important to enhance women's access to the media and income, promote family planning and contraception, and improve men's educational status in order to improve the status of women's empowerment.
Determinants of Women Empowerment in Bishoftu Town; Oromia Regional State of ...
Effect of Clariodeoglomusclariodeorum on morphology and abundant of carrot root hairs in vitro
1. International journal of Horticulture, Agriculture and Food science(IJHAF) [Vol-2, Issue-3, May-Jun, 2018]
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Effect of Clariodeoglomusclariodeorum on
morphology and abundant of carrot root hairs in
vitro
Nikoo Rokni1
*, Seyed Esmaeil Razavi2
, Kambiz Mashayekhi3
1
Master student of Plant Pathology. Gorgan University of agricultural sciences and natural resources. Collage of Plant
Production. Department of Plant Protection, Iran
2
Assistant Professorof Plant Pathology. Gorgan University of agricultural sciences and natural resources. Collage of Plant
Production. Department of Plant Protection, Iran
3
Associate Professor of Horticulture. Gorgan University of agricultural sciences and natural resources. Collage of Plant
Production. Department of Horticulture, Iran
Email of Corresponding Author: esfnikoor2@gmail.com. Phone: 989135751285.
Abstract— The roothairs are important components of the
root for absorbing nutrients for plants and also secreting the
plant-produced secretes. Morphology and their number are
influenced by various environmental and internal factors and
are regulated by them. Mycorrhizal fungi are established
through the root and their presence in the root can affect
root’s physical and chemical properties.The aim of this study
was to evaluate the effect of the presence of the fungus in the
roots on the characteristics of the capillary roots.In order to
remove the effect of other microorganisms on the results of
the experiment, this experiment was performed in vitro on the
roots of the carrot secondary phloem tissue culture. In this
experiment, the fungus could affect the characteristics of the
root hairs: their number (22% decrease in the root hairs
number in the root hair area of the root) and their length (A
decrease of 21.3% in the length of capillary roots in
mycorrhizal plants). These changes in the characteristics of
capillary roots were also caused by the presence of fungal
structures in the roots as well as by the decrease in the
production of strigolactones. In this study, changes in the
production of strigolactones calculated by using their effect
on seed germination of Phelipancheaegyptiaca.
Keywords— Phelipancheaegyptiaca, Strigolactones,
Capillary root’s length.
Highlight:
According to our results, the presence of mycorrhizal fungi
can act as an alternative to root hairs. And their wide hyphae
of these fungi can have the same function or better than the
capillary roots for the plant. An important point in this regard
is the sensitivity of the roots of plants from tissue culture
after leaving the desirable condition of in vitro and entering
the new environmental condition such as a field. Meanwhile,
using Mycorrhizal fungi can provide a stronger guarantee for
the ability of the roots of these tissue culture derived plants
in the presence of mycorrhizal fungi inside them, in an out of
glass environment.
Abbreviation:
In this experiment, which was carried out in tissue culture
condition, the effect of mycorrhizal fungi on the number and
length of capillary roots was investigated. The results showed
that the presence of fungi in the root of the plant affected the
amount of strigolactones production and reduced it.
Following a decrease in the production of strigolactones, the
number and length of root hairs also decreased. It was shown
in this experiment that the presence of mycorrhizal fungi can
act as an alternative to root hairs. And the wide hyphae of
these fungi can have the same function or better than the
capillary roots for the plant. An important point in this regard
is the sensitivity of the roots of tissue culture plants after
leaving the in vitro condition and entering the field.
Meanwhile, using Mycorrhizal fungi can provide a stronger
guarantee for the ability of the roots of these plants in the
presence of mycorrhizal fungi inside them, in an out of in
vitro condition.
I. INTRODUCTION
Mycorrhizaم arbuscular fungi (AM) belong to the
Glomeromycota branch andGlomales class(Schusler,
Schwarzott, & Walker, 2001). AM fungi are the compulsory
coexistence of plants, and both sides benefit from this
bilateral cooperation between fungi and plant (Smith & Read,
2008). In addition to enhancing access to mineral elements,
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almonds have other advantages such as increased tolerance to
drought and heavy metals, protection against pathogens and
plant breeding for plant host (Gange & West, 1994; Joner,
Briones, & Leyval, 2000; Khan, Kuek, Chaudhry, Khoo, &
Hayes, 2000; Augé, 2001). Also, they affect plant diversity
and population structure and contribute to the growth and
deployment of seedlings(Grime, Mackey, Hillier, & Read,
1987; Klironomos, 2003; Van Der Heijden, 2004; Van Der
Heijden, et al., 2006). In the relationship between plant and
AM fungi, there is a certain Chemical communication. These
chemical connections include the release of a number of
signals released into the environment from the plant and the
production of signals emitted by the fungus(Oldroyd, 2013).
The response of different plant species to colonization by
Mycorrhizal fungi is different. This response usually depends
on the degree of dependence of the plant species on the
mycorrhizal communication. According to this, the
morphological characteristics of the root will
change(Hayman, 1983). Therefore, the fungus associated
with the root can be related to the plant. In the meantime, the
fungus needs to change the specific characteristics of the root
so that it can produce its effective and beneficial effect on the
plant. In this research, we have tried to determine the effect
of these coexist fungi on the root by examining some
morphological and chemical characteristics of the root.
II. MATERIALS AND METHODS
Isolation of mycorrhizal fungus:
Soil and root samples were collected from the rhizosphere
area of carrot root in carrot fields in Isfahan in the fall of
2015. Spores of mycorrhizal fungi were isolated from the soil
and were identified using articles and information founded
on valid sites (www.zor.zut.edu.pl and www.invam.caf.wvu).
From isolated spores, Clariodeoglomusclariodeumspecies
was selected and propagated on corn roots in pearl perlite
pots. The spores were then placed on a solid WA medium for
germination. Germinated spores were removed with one
millimeter of the surrounding culture medium and placed
next to the root of the carrot culture from the previously
cultured tissue culture in MS medium under conditions of in
vitro to penetrate the root.
Data analysis:
This experiment was conducted in a completely randomized
design with data analysis using IBM SPPSS Statistics
software.
Measuring the amount of strigolactone in the root
To determine the production of strigolactones in the carrot
roots, we used strigolactones in stimulating seed germination
of the Phelipancheaegyptiaca parasite plant. This experiment
is based on an experience conducted by Araco et al in 2013.
Carrot root extract was applied to the seeds. In this
experiment, the germination inducer, GR24 (10-10 and 10-11
M), was used as a positive control and water was used as a
negative control. As a point, the germination rate of carrot
root extract is always lower than that of GR24.
III. RESULTS
Number of root hairs in the mycorrhizal and non-mycorrhizal plants in vitro:
Fig.1: The number of root hairs in 100 micrometers of the root hairs area of root (P <0.05). 1) Mycorrhizal plant, 2) Non-
mycorrhizal plant..
b
a
0
10
20
30
40
50
60
1 2
Thenumberofroothairs
Treatments
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As shown in figure 1, the presence of fungus in the root causes a 22% reduction in the number of roots hairs. According to the
data analysis, this decrease was significant at the level of 0.05% .
The length of capillary roots in mycorrhizal and non-mycorrhizal plants:
Fig.2: Comparison of the length of root hairs in mycorrhizal and non-mycorrhizal plants. 1) Mycorrhizal plant, 2) Non-
mycorrhizal plant. (95% confidence interval of the difference).
Data analysis results showed a decrease of 21.33% over the root hairs length in the mycorrhizal plant (Figure 2). According to the
data analysis, this decrease was significant at the probability level of 0.05%.
Hair root area in carrot root under an optical microscope:
Fig.3: The number of root hairs in mycorrhizal and non-mycorrhizal plants under optical microscopy (Model BM-22). a) Non-
mycorrhizal plant, you can see here the abundant number of root hairs and their high length of them in absent of mycorrhizal
fungus, b, c, and d) Mycorrhizal plant. Here you can see a small number of root hairs in the mycorrhizal plant. They are also
much smaller than non-mycorrhizal plants in length.
b
a
0
10
20
30
40
50
60
70
1 2
Lenghofroothair(µm)
Treatments
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The effect of Mycorrhiza fungus on the rate of production of strigolactones:
Fig.4: Percentage of P. aegypicae seed germination in mycorrhizal and non-mycorrhizal plants. 1) Water (Control -), 2) GR24
(Control+) 10-10
M, 3) GR24 (Control+) 10-11
M, 4) Non-mycorrhizal plant, 5) Mycorrhizal plant.
As shown in Figure 4, the presence of the extract of carrot
root has a significant effect on germination of P. aegypicae
seeds, however, this increase in germination in the
mycorrhizal plant is significantly lower than that of the non-
mycorrhizalplant.
IV. DISCUSSION
According to earlier studies, as with the results of this
experiment, there is always a correlation between the amount
of capillary roots of the plants and their need for mycorrhizal
coexistence. The presence of mycorrhizal fungi in the plant
directly reduced the number of capillary roots and their
length. Their absence resulted in normal and natural growth
of capillary roots. Akiyama et al. (2010) found that there is a
linear relationship between the root structure and the
mycorrhizal roots. The length of the capillaries root and their
abundance in the root is a good guide to detect the degree of
dependence of the plant on the mycorrhizal coexistence, as
shown in Figure 2 and 3, in the mycorrhizal plants, the length
of the capillary root, as well as their abundance in this area
had a significant decrease. Plants with large roots and a
smaller number of capillary roots, such as citrus, strongly
depend on mycorrhizal fungi. On the other hand, plants such
as Gramineae, having narrower roots and more root hairs,
have reported less interdependence with these types of fungi,
according to reports. Plants that have more affinity for the
coexistence have a roughly rooting system, the root-to-shoot
ratio is almost constant, and a much smaller number of root
hairs formed(Hetrick, 1991). In a number of studies, it has
been pointed out that a large root system with long capillary
roots can be sufficient for the plant as mycorrhizal plants do,
and reduces the plant's dependence on these fungi
(Schweiger, Robson, & Barrow, 1995; Baylis, 1970). In this
experiment, it can be seen that root characteristics can be
changed in relation to the presence of mycorrhizal fungus.
However, external hyphae of these fungi, even when they
have the same length with capillary roots, can be much more
efficient in absorbing nutrients and water than root hairs.
This is due to the fact that these fungi have the ability to
penetrate into smaller pores of the soil, and also hyphae do
not compete in phosphorus absorption, while adjacent roots
compete themselves in sources(Baldwin, Tinker, & Nye,
1972; Barber, 1995). Lee et al. (2014) obtained similar
results to the results of this experiment and found that
mycorrhizal roots could act as a substitute for capillary roots.
According to this, it is possible to justify the reducing inthe
number of root hairs in the presence of mycorrhizal
fungi.Baylis (1970) also said that plants with shorter
capillary roots are heavily dependent on arbuscular fungi, in
our experiment, with root colonization, in addition to
reducing the number of capillary roots in this area, their
length also diminished. Crush (1974) also reported that the
decrease in dependence on arbuscular fungi resulted in an
increase in the length of capillary root in the three
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leguminous plants. Manjunatfa andHahte(1991) also found
that capillary root length is one of the most important criteria
for determining the dependence of the plant on arbuscular
fungi, which is identical to the results of this experiment.
The influence of existence of mycorrhizal fungi on the
amount of strigolactones production:
Changes in root morphological characteristics, such as the
number and length of capillary root that were examined in
this experiment, are due to several factors. These factors
include the effect of the existence of fungal structures in the
root and changes in the root growth factors. One of the
factors affecting roots is the amount of strigolactones
production(Kapulnik, et al., 2011). In this experiment, it was
shown that mycorrhizal fungi can affect the amount of
strigolactones production and reduce their amount.
Strigolactones are newly discovered metabolites that are
considered to be phytohormones. These hormones play a role
in plant biology. And according to studies, Strigolactones are
known to play a role in the morphology and architecture of
the plant. They affect the formation of lateral roots and
capillary roots and lift up their length. So they adjust the
growth of the plant in different environmental conditions
(Gomez-Roldan, et al., 2008; Umehara, et al., 2008; Vogel,
et al., 2010). Therefore,they are as the negative regulators of
the lateral roots and the positive regulator of the lengthening
of the capillary roots under stress conditions (for example,
low levels of nutrients), respond to growth conditions
(Kapulnik, et al., 2011).In non-stress conditions, the presence
of these fungi within the root reduces the production of
strigolactones. In the same work, the presence of mycorrhizal
fungi in the pea reduced the germination percentage of
Orobanche and Phelipanche species. That it showed a
reduction in strigolactones(Fernandez-Aparicio, GArcia-
Garrido, Ocampo, & Rubiales, 2010). In addition,
previouslyhad been shown that in tomato mycorrhizal plants,
seed yield increased and analyzes showed that this increase
was related to a decrease in strigolactones(Fernandez-
Aparicio, GArcia-Garrido, Ocampo, & Rubiales, 2010).
Therefore, the decrease in the production of strigolactones by
the fungus appears to be a phenomenon that is different in
different plant species. It seems that the regulation and
controlling of root colonization by the fungus was done in
this way, and this is a plant strategy that prevents excessive
colonization. In some cases, the plant is able to respond to
the presence of G. intraradices in the form of secretion of
strigolactones. Auxin and Ethylene as phytohormones play a
key role in regulating lateral roots formation and also the
development of capillary roots that affect the level of Pi
deficiency(Rubio, et al., 2009). This is similar to the effect
that this Pi deficiencyhas on the rate of colonization by the
arbuscular fungi. It has recently been shown that
strigolactones, along with Auxin, play a role in the
development of lateral roots and branches in Arabidopsis.
This depends on the level of Pi in the growth medium
(Ruyter-Spira, et al., 2011).
Kapulnik et al, (2011) also obtained similar results with our
experiment and concluded that strigolactones have a direct
effect on capillary rootsmorphology and abundance.
In the meantime, it may be necessary to say that other
microorganisms that have a relationship with the roots of
plants, especially microorganisms that have a symbiotic
relationship, can have a significant effect on the number and
morphology of the root and especially root hairs. For
example, in a study conducted by Heidstra et al in 1997,
Rhizobium in legumecaused an increase in the number of
root hairs.
V. CONCLUSION
In this experiment, which was carried out in tissue culture
condition, the effect of mycorrhizal fungi on the number and
length of capillary roots was investigated. The results showed
that the presence of fungi in the root of the plant affected the
amount of strigolactones production and reduced it.
Following a decrease in the production of strigolactones, the
number of root hairs also decreased by 22%. In addition to
numbers, their length also decreased. It was shown in this
experiment that the presence of mycorrhizal fungi can act as
an alternative to root hairs. And the wide hyphae of these
fungi can have the same function or better than the capillary
roots for the plant. An important point in this regard is the
sensitivity of the roots of tissue culture plants after leaving
the in vitro condition and entering the field. Meanwhile,
using Mycorrhizal fungi can provide a stronger guarantee for
the ability of the roots of these plants in the presence of
mycorrhizal fungi inside them, in an out of in vitro condition.
ACKNOWLEDGMENTS
This work was supported by Gorgan University of
agricultural sciences and natural resources, Iran.
REFERENCES
[1] Akiyama, K., Tanigawa, F., Kashihara, T., & Hayashi,
H. (2010). Lupin pyranoisoflavones inhibiting hyphal
development in arbuscular mycorrhizal fungi.
Phytochemistry, 71(16), 1865-1871.
[2] Aroca, R., Ruiz-Lozano, J. M., Zamarreño, Á. M., Paz,
J. A., García-Mina, J. M., Pozo, M. J., & López-Ráez, J.
A. (2013). Arbuscular mycorrhizal symbiosis influences
strigolactone production under salinity and alleviates
6. International journal of Horticulture, Agriculture and Food science(IJHAF) [Vol-2, Issue-3, May-Jun, 2018]
https://dx.doi.org/10.22161/ijhaf.2.3.2 ISSN: 2456-8635
www.aipublications.com/ijhaf Page | 37
salt stress in lettuce plants. Journal of Plant Physiology,
170(1), 47-55.
[3] Augé, R. M. (2001). Water relations, drought and
vesicular-arbuscular mycorrhizal symbiosis.
Mycorrhiza, 11(1), 3-42.
[4] Baldwin, J. P., Tinker, P. B., & Nye, P. H. (1972).
Uptake of solutes by multiple root systems from soil II.
The theoretical effects of rooting density and pattern on
uptake of nutrients from soil. Plant and Soil, 693-708.
[5] Barber, S. A. (1995). Soil nutrient bioavailability: a
mechanistic approach. New York.: Wiley-Interscience.
[6] Baylis, G. T. (1970). Root hairs and phycomycetous
mycorrhizas in phosphorus-deficient soil. Plant and
soil, 33(1-3), 713-716.
[7] Crush, J. R. (1974). PLANT GROWTH RESPONSES
TO VESICULAR‐ARBUSCULAR MYCORRHIZA
VII. GROWTH AND MODULATION OF SOME
HERBAGE LEGUMES. New phytologist, 73(4), 743-
749.
[8] Fernandez-Aparicio, M., GArcia-Garrido, J. M.,
Ocampo, J. A., & Rubiales, D. (2010). Colonisation of
field pea roots by arbuscular mycorrhizal fungi reduces
Orobanche and Phelipanche species seed germination.
Weed research, 50(3), 262-268.
[9] Gange, A. C., & West, H. M. (1994). Interactions
between arbuscular mycorrhizal fungi and
foliar‐feeding insects in Plantago lanceolata L. New
Phytologist, 128(1), 79-87.
[10]Gomez-Roldan, V., Fermas, S., Brewer, P. B., Puech-
Pagès, V., Dun, E. A., Pillot, J. P., . . . Bouwmeester, H.
(2008). Strigolactone inhibition of shoot branching.
Nature, 455(7210), 189.
[11]Grime, J. P., Mackey, J. M., Hillier, S. H., & Read, D.
J. (1987). Floristic diversity in a model system using
experimental microcosms. Nature, 328(6129), 420.
[12]Hayman, D. S. (1983). The physiology of vesicular–
arbuscular endomycorrhizal symbiosis. canadian
Journal of Botany, 61(3), 944-963.
[13]Heidstra, R., Yang, W. C., Yalcin, Y., Peck, S., Emons,
A., & Bisseling, T. (1997). Ethylene provides positional
information on cortical cell division but is not involved
in Nod factor-induced root hair tip growth in
Rhizobium-legume interaction. Development, 124(9),
1781-1787.
[14]Hetrick, B. A. (1991). Mycorrhizas and root
architecture. Experientia, 47(4), 355-362.
[15]Joner, E. J., Briones, R., & Leyval, C. (2000). Metal-
binding capacity of arbuscular mycorrhizal mycelium.
Plant and soil, 226(2), 227-234.
[16]Kapulnik, Y., Delaux, P. M., Resnick, N., Mayzlish-
Gati, E., Wininger, S., Bhattacharya, C., . . . Beeckman,
T. (2011). Strigolactones affect lateral root formation
and root-hair elongation in Arabidopsis. Planta, 233(1),
209-216.
[17]Khan, A. G., Kuek, C., Chaudhry, T. M., Khoo, C. S.,
& Hayes, W. J. (2000). Role of plants, mycorrhizae and
phytochelators in heavy metal contaminated land
remediation. Chemosphere, 41(1-2), 197–207.
[18]Klironomos, J. N. (2003). Variation in plant response to
native and exotic arbuscular mycorrhizal fungi.
Ecology, 84, 2292–2301.
[19]Lee, M. R., Tu, C., Chen, X., & Hu, S. (2014).
Arbuscular mycorrhizal fungi enhance P uptake and
alter plant morphology in the invasive plant
Microstegium vimineum. Biological invasions, 16(5),
1083-1093.
[20]Manjunath, A., & Habte, M. (1991). Root
morphological characteristics of host species having
distinct mycorrhizal dependency. Canadian Journal of
Botany, 69(3), 671-676.
[21]Oldroyd, G. E. (2013). Speak, friend, and enter:
signalling systems that promote beneficial symbiotic
associations in plants. Nature Reviews Microbiology,
11(4), 252.
[22]Rubio, V., Bustos, R., Irigoyen, M. L., Cardona-López,
X., Rojas-Triana, M., & Paz-Ares, J. (2009). Plant
hormones and nutrient signaling. Plant molecular
biology, 69(4), 361.
[23]Ruyter-Spira, C. K., van Zeijl, A., van Bezouwen, L.,
de Ruijter, N., ..., & Verstappen, F. (2011).
Physiological effects of the synthetic strigolactone
analog GR24 on root system architecture in
Arabidopsis: another belowground role for
strigolactones? Plant physiology,, 155(2), 721-734.
[24]Schusler, A., Schwarzott, D., & Walker, C. (2001). A
new fungal phylum, the Glomeromycota: phylogeny
and evolution. Mycological research, 105(12), 1413-
1421.
[25]Schweiger, P. F., Robson, A. D., & Barrow, N. J.
(1995). Root hair length determines beneficial effect of
a Glomus species on shoot growth of some pasture
species. New Phytologist, 131(2), 247-254.
[26]Smith, S. E., & Read, D. J. (2008). Mycorrhizal
symbiosis. Academic press.
[27]Umehara, M., Hanada, A., Yoshida, S., Akiyama, K.,
Arite, T., Takeda-Kamiya, N., . . . Kyozuka, J. (2008).
Inhibition of shoot branching by new terpenoid plant
hormones. Nature, 455(7210), 195.
7. International journal of Horticulture, Agriculture and Food science(IJHAF) [Vol-2, Issue-3, May-Jun, 2018]
https://dx.doi.org/10.22161/ijhaf.2.3.2 ISSN: 2456-8635
www.aipublications.com/ijhaf Page | 38
[28]Van Der Heijden, M. G. (2004). Arbuscular
mycorrhizal fungi as support systems for seedling
establishment in grassland. Ecology letters, 7(4), 293-
303.
[29]Van Der Heijden, M. G., Streitwolf‐Engel, R., Riedl,
R., Siegrist, S., Neudecker, A., Ineichen, K., . . .
Sanders, I. R. (2006). The mycorrhizal contribution to
plant productivity, plant nutrition and soil structure in
experimental grassland. New Phytologist, 172(4), 739-
752.
[30]Vogel, J. T., Walter, M. H., Giavalisco, P.,
Lytovchenko, A., Kohlen, W., Charnikhova, T., . . .
Fernie, A. R. (2010). SlCCD7 controls strigolactone
biosynthesis, shoot branching and mycorrhiza‐induced
apocarotenoid formation in tomato. The Plant Journal,
61(2), 300-311.