Biosolubilization of mineral insoluble phosphates in fluidized bed bioreactor

2. BIOSOLUBILIZATION IN FLUIDIZED BED BIOREACTOR
PRESENTED BY, GUIDED BY,
HARITHA K M LEKSHMI MISS
ROLL NO 7111 ASST PROFESSOR
BT AND BCE BT AND BCE
SREE BUDDHA COLLEGE OF ENGINEERING,PATTOOR

5.  The world population increasing day by day.
 Hence there is need for plenty of food crops to meet the
requirement.
 Crops need several nutrients to reach their maximum potential
yield.

6.  Phosphorus is an essential macronutrient for plants.
 Phosphate fertilizers is easily precipitated into insoluble
forms.
 Solubilisation of inorganic phosphates by
microorganisms.

7.  These microorganisms was entrapped in alginate and
polyacrylamide gels.
 Used for solubilizing mineral insoluble phosphates in
fluidised bed bioreactor.
 ADVANTAGES
Reusability of the same biocatalyst
Control of reactions
No contamination of products

8.  It plays an important role in metabolism of crop plants.
 About 10 to 25% of fertilizer phosphorous is acquired by plants.
 It is the constituent of various cellular functions or activities such as
cell division ,development ,photosynthesis , breakdown of sugars etc.
 Plants deficient in P, shows retarded growth.

9.  Phosphorous is taken up by the plants
from soils and it is consumed by
animals.
 Return to soil as organic residues.
 Conversion of organic phosphate to
inorganic phosphate is known as
Mineralization

10.  In soil phosphate solubilizing bacteria constitute 1 to
50%,while phosphate solubilizing fungi are 0.1 to 0.5%.
 Phosphate solubilizing activity is greater for fungi than
bacteria.
1 Repeated sub culturing
2 Produce more acids
3 Traverse more distances

11. BACILLUS
PSEUDOMONOS

12. Aspergillus
awamori
Aspergillus
conidia
Aspergillus
colony

13. Aspergillus
fruiting body
Penicillium Trichoderma
viridi

15.  Phosphate solubilizing microorganisms convert insoluble
phosphates into soluble forms .
 Through the processes of acidification, by the production of organic
acids, production of acid and alkaline phosphatases.
 These organic acids can either dissolve phosphates as a result of
anion exchange or can chelate Ca,Fe or Al ions associated with
phosphates.

16.  Grow bacteria/fungi in specified media containing insoluble
phosphate.
 Incubate and observe the clearing zone around colonies.
 Quantify the phosphorous dissolved by the specified
organism.
 Select efficient culture based on above criteria.

18.  Conversion of inorganic
compounds to organic
compounds by
microorganisms.
 It is prevented from being
accessible to plants.
 Opposite to mineralization

20. ENTRAPMENT IN CALCIUM GEL:
 100ml sterile sodium alginate + 2g fungal biomass
 Mixture was extruded into 150 mM Calcium Chloride, forming
beads of 5mm diameter

21. ENTRAPEMENT IN POLYACRYLAMIDE GEL:
 2g fungal biomass + 78ml Tris-HCL buffer+20 ml bisacrylamide
solution+1ml ammonium persulfate
 Polymerisation is initiated adding 100μl of N,N,N’,N’- tetramethyl-
ethylenediamine

22. FLUIDISED BED
BIOREACTOR
 Small particles are suspended
 Upward motion of reacting fluid
 Used when intimate content
between the solid particles and
fluid is required.
 Solid particles could be
flocculated cells
 Fluid could be fermentation
broth.

23.  Fluidized bed bioreactors composed of 500 ml conical flask containing
the immobilized fungal biomass
 Suspented in 200 ml of NBRIP liquid medium and supplemented by 5g/l
of DCP
 Placed in a rotary shaker at 25°C
 Fluidisation was assured by stirring at a rate of 120rpm
 Phosphate solubilisation rate was followed according to time in
bioreactor.
 Supernatants was analysed spectrophotometrically to determine amount
of soluble phosphorous

24.  Longevity of solubilisation activity of the immobilised
fungal biomass was investigated in repeated batch
solubilisation tests.

26. Soil application method

27.  Greater population
 Faster action
 Withstand low moisture content
 Improve plant growth and yield
 Increaasing fixed phosphorous in soil

28.  Phosphate solubilizing bacteria not only release phosphate
but also biological components such as auxin,gibberellic acid
as well as vitamins
 The poor survival and colonization
 Strong competition from native microbes
 Availability of inadequate nutrients

29.  Bioconversion occurs at a low temperature and is more
selective to phosphate extraction than conventional processes.
 Biophosphoros fertilization is an achievable goal that tends itself
well to the sustainable agricultural production.
 Apart from phosphate solubilizing abilities, some of these
microorganisms can benefit plant growth by several different
mechanisms such as enhancing nitrogen fixation, plant hormone
production .

30. REFERENCES
 Zeroual Y, Chadghan R, Hakam A, Kossir A (2012) Biosolubilization of Mineral Insoluble
Phosphates by Immobilized Fungi (Aspergillus niger) in fluidised bed bioreactor. J JBiotechnol
Biomaterial s6 dx:htpp//.doiorg/104172/2155-952X,s6-004
 R.Jain et al./Applied Soil Ecology 46 (2010) The evaluation of free encapsuled Aspergillus
awamori for phosphate solubilization fermentation and soil plant system. Applied soil ecology
46 90-94
 Rashi Awasthi R Tewari and Harsh Nayyar(2011)Synergy between plants and p- solubilizing
microbes in soils:Effect of growth and physiology of crops.International research journals
vol.2(12)pp 484-504
 Ahmad Ali Khan and Ghulam Jilani(2009)Phosphorous solubilizing bacteria: occurrence ,
mechanisms and their role in crop production. J.Agric.biol,.sci 1(1)(48-58)
 Praveen M Patil, V B Kuligod (2012)Effect of phosphate solubilizing fungi and phosphorus
levels on growth, yield and nutrient content in maize.Karnataka J. Agric.sci,25(1)(58-62)
 Satya Sunder Bhattacharya and Soma Barman (2013)Phosphate solubilizing ability of
Emericella nidulans strain isolated from vermicompost Indian journal of experimental
biology.Indian Journal of Experimental Biology vol 51,October 2013,pp.840-848