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A BRIEF
INTRODUCTION TO
MYCORRHIZA AND
ITS ROLE IN
PHOSPHOROUS
UPTAKE
Fariha Ilyas
CONTENTS
Introduction
Types of
mycorrhiza
• Ectomycorrhiza
• Endomycorrhiza
Types of
Endomycorrhiza
• Comparison
Significance of
Mycorrhiza
• Methods of
Nutrient uptake in
mycorrhizal plant
• Factors affecting
nutrient uptake in
mycorrhizal plants
Role of
Mycorrhiza
in P uptake
MYCORRHIZA
• Literally translate to ‘Fungus-Root’
• A symbiotic relationship between roots of a plant and a
fungus that colonize plant roots.
• Fungus facilitates nutrients and water uptake in plants
• Plant provides food/ Sugars.
(Solaiman and Saito, 1997)
Internal structure of root Root
Mycorrhiza
Root
cell
Hartig
network
ECTOMYCORRHIZA
ECTOMYCORRHIZAA
• Fungi make interwoven
mantle of hyphae around the
root surface.
• Fungi penetrate the root
intercellular space of cortex.
• Includes plants of high global
and economical value
(wooden trees, forests, shrubs
etc.)
ECTOMYCORRHIZA
• Highly competitive in nutrient acquisition, secrete
number of enzyme
– Degrade little polymers.
• Lost their ability to degrade plant cell wall
– Restrict their penetration in root intercellular.
• Can live as symbionts with plant roots and as
facultative saprotrophs in soil.
ENDOMYCORRHIZA
• Live within cortical cells and also have inter-
cellular growth.
• Morphologically unaltered with plants for more
than 400 million years ago.
• Unique in its structure, lifestyle and age
ENDOMYCORRHIZA
– Formation of arbuscules and
vesicles.
– Branched haustorial structures within
the cortical cells
– Coenocytic
– Asexual but exchange genetic
material b/w closely related fungi
Unique characters
TYPES OF ENDOMYCORRHIZA
Endomycorr
hiza
Orchid
Myc.
Arbuscu
lar Myc.
Ericaceou
s Myc.
Arboutid
Myc.
Found in Orchid plants
Similar to EM but
can penetrate in root
cortical cells Found
in trees & cooler region
Plants of the order
Ericales & inhospitable,
acidic environments
Form arbuscules
High Affinity for
P uptake
SIGNIFICANCE OF MYCORRHIZA
• Only Bio-fertilizer.
• Non-specific organism
– a single sp. colonize 85% land plants.
• Increases photosynthesis rate
• Broad ecological adaptability
– Deserts, arctic, temperate, tropical and other.
• 50% reduction in chemical fertilizer application.
• lower carbon cost as compared to roots
SIGNIFICANCE OF MYCORRHIZA
• Tolerance against stress conditions
– e.g. salinity, drought enhance the chance of plant survival.
• Resistance to soil & root-borne pathogens
– a potential disease control agent
• Soil conservation & soil structure stabilization
• Avoid Arsenate uptake
• Better uptake of nutrients like P & other immobile
nutrients
– e.g. Zn, Co, Mg, Fe, Cu.
Scheme summarizing the main nutrient exchange processes
https://microbewiki.kenyon.edu/index.php/File:Nutrients.jpg
ROLE OF
MYCORRHIZA IN
PHOSPHOROUS
UPTAKE BY
PLANTS
P PROBLEM IN OUR SOIL
Macro
nutrient
Fixation
Less
soluble
Erosion
Crop
Removal
Adaptation of Plant for P-uptake
• Strategy # 1
– Formation of dense
cluster roots
Cluster roots (Hakea prostrata)
(UWA Science Global)
Adaptation of Plant for P-uptake
• Strategy # 2
AM symbiosis more
uptake
How Mycorrhizal Plants Take Up
Nutrients?
• Mycorrhizal plants take up nutrients from
soil via two pathways:
– Plant pathway
– Mycorrhizal pathway
Depletion Zone?
• Nutrients rapidly removed from the soil
solution than they can be replaced by
diffusion.
• Phosphate is poorly mobile in soil
 a narrow depletion zone develops close to root.
• Low Diffusion rate
Different Mechanisms of Mycorrhiza
which helps in nutrient uptake
• Formation of polyphosphates in the hyphae
– Maintaining low internal phosphate (Pi) concentrations
• Small hyphal diameter.
– 2-6 times higher P influx rate per unit length of hyphae
• Production of extracellular acid phosphatases
– Catalyze the release of P from organic complexes in the soil
– In EM & Ericoid only
(Jakobsen et al., 1992 & Jungk and Claassen, 1989)
MECHANISM OF P-MINERALIZATION
Phytate
(Organic phosphorus Po)
Inorganic phosphorus
Mineralization
Mycorrhiza
Uptake by
plant
Soil Solution
Secrete enzyme
Enhance bacteria
Phytases
Solubilize P
Uptake by Mycorrhizae
Indirect
modification
• Greater desorption
of adsorbed P
• Large surface area
• Enhance P uptake form
Poorly soluble
Ca-phosphate
Direct
Modification
• Production of Phosphatases
• Solubilization of Mineral
Phosphate
• Produce Ca-oxalate, which
can bind Fe, Al
• Enhance production of
chelates, organic acids,
siderophores
Storage of Adsorbed P
P is continuously absorbed by plants.
– Soluble Orthophosphate (Harely and Loughman, 1963)
– Soluble Polyphosphate (Martin et al., 1983)
– Polyphosphate granules (40%) (Chilvers and Harley, 1980)
Mycorrhizal Factors Affecting Nutrients
Uptake
 Either it is ECM or AM association
Only mycorrhizal pathway in ECM
Both pathways in AM
 Plant & Fungal species
 Fungal species effect on activity of pathway
 Efficiency with which both partners interact & exchange
Factors Affecting on Mycorrhizal
Growth
Enhance Colonization
• Less fertile soil
• Natural ecosystem
• Coarse texture soil
• Slow root growth
Decrease colonization
• High P conc.
• Agronomic practices
• Long fallow period
• Tillage
• Fine texture soil
• Rapid root growth
Is There Any Negative
Effect of Mycorrhiza on
Plant?
-
High C
uptake by
myc.
Unhealthy
plant
High Myc.
growth
Very low P in
soil
(Schroeder and Janos 2004; Smith and Smith 1996; West et al. 1993; Whitbeck 2001) & Morgan et al. 2005
Apply poorly available
source of Phosphorous
NaHCO3-extractable P levels
typically between 5 & 20 mg/kg
introduction to mycorrhizae and its role in P uptake

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introduction to mycorrhizae and its role in P uptake

  • 1.
  • 2. A BRIEF INTRODUCTION TO MYCORRHIZA AND ITS ROLE IN PHOSPHOROUS UPTAKE Fariha Ilyas
  • 3. CONTENTS Introduction Types of mycorrhiza • Ectomycorrhiza • Endomycorrhiza Types of Endomycorrhiza • Comparison Significance of Mycorrhiza • Methods of Nutrient uptake in mycorrhizal plant • Factors affecting nutrient uptake in mycorrhizal plants Role of Mycorrhiza in P uptake
  • 4. MYCORRHIZA • Literally translate to ‘Fungus-Root’ • A symbiotic relationship between roots of a plant and a fungus that colonize plant roots. • Fungus facilitates nutrients and water uptake in plants • Plant provides food/ Sugars. (Solaiman and Saito, 1997)
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  • 7. Internal structure of root Root Mycorrhiza Root cell Hartig network ECTOMYCORRHIZA
  • 8. ECTOMYCORRHIZAA • Fungi make interwoven mantle of hyphae around the root surface. • Fungi penetrate the root intercellular space of cortex. • Includes plants of high global and economical value (wooden trees, forests, shrubs etc.)
  • 9. ECTOMYCORRHIZA • Highly competitive in nutrient acquisition, secrete number of enzyme – Degrade little polymers. • Lost their ability to degrade plant cell wall – Restrict their penetration in root intercellular. • Can live as symbionts with plant roots and as facultative saprotrophs in soil.
  • 10. ENDOMYCORRHIZA • Live within cortical cells and also have inter- cellular growth. • Morphologically unaltered with plants for more than 400 million years ago. • Unique in its structure, lifestyle and age
  • 11. ENDOMYCORRHIZA – Formation of arbuscules and vesicles. – Branched haustorial structures within the cortical cells – Coenocytic – Asexual but exchange genetic material b/w closely related fungi Unique characters
  • 12. TYPES OF ENDOMYCORRHIZA Endomycorr hiza Orchid Myc. Arbuscu lar Myc. Ericaceou s Myc. Arboutid Myc. Found in Orchid plants Similar to EM but can penetrate in root cortical cells Found in trees & cooler region Plants of the order Ericales & inhospitable, acidic environments Form arbuscules High Affinity for P uptake
  • 13. SIGNIFICANCE OF MYCORRHIZA • Only Bio-fertilizer. • Non-specific organism – a single sp. colonize 85% land plants. • Increases photosynthesis rate • Broad ecological adaptability – Deserts, arctic, temperate, tropical and other. • 50% reduction in chemical fertilizer application. • lower carbon cost as compared to roots
  • 14. SIGNIFICANCE OF MYCORRHIZA • Tolerance against stress conditions – e.g. salinity, drought enhance the chance of plant survival. • Resistance to soil & root-borne pathogens – a potential disease control agent • Soil conservation & soil structure stabilization • Avoid Arsenate uptake • Better uptake of nutrients like P & other immobile nutrients – e.g. Zn, Co, Mg, Fe, Cu.
  • 15. Scheme summarizing the main nutrient exchange processes https://microbewiki.kenyon.edu/index.php/File:Nutrients.jpg
  • 17. P PROBLEM IN OUR SOIL Macro nutrient Fixation Less soluble Erosion Crop Removal
  • 18. Adaptation of Plant for P-uptake • Strategy # 1 – Formation of dense cluster roots Cluster roots (Hakea prostrata) (UWA Science Global)
  • 19. Adaptation of Plant for P-uptake • Strategy # 2 AM symbiosis more uptake
  • 20. How Mycorrhizal Plants Take Up Nutrients? • Mycorrhizal plants take up nutrients from soil via two pathways: – Plant pathway – Mycorrhizal pathway
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  • 22. Depletion Zone? • Nutrients rapidly removed from the soil solution than they can be replaced by diffusion. • Phosphate is poorly mobile in soil  a narrow depletion zone develops close to root. • Low Diffusion rate
  • 23. Different Mechanisms of Mycorrhiza which helps in nutrient uptake • Formation of polyphosphates in the hyphae – Maintaining low internal phosphate (Pi) concentrations • Small hyphal diameter. – 2-6 times higher P influx rate per unit length of hyphae • Production of extracellular acid phosphatases – Catalyze the release of P from organic complexes in the soil – In EM & Ericoid only (Jakobsen et al., 1992 & Jungk and Claassen, 1989)
  • 24. MECHANISM OF P-MINERALIZATION Phytate (Organic phosphorus Po) Inorganic phosphorus Mineralization Mycorrhiza Uptake by plant Soil Solution Secrete enzyme Enhance bacteria Phytases Solubilize P
  • 25. Uptake by Mycorrhizae Indirect modification • Greater desorption of adsorbed P • Large surface area • Enhance P uptake form Poorly soluble Ca-phosphate Direct Modification • Production of Phosphatases • Solubilization of Mineral Phosphate • Produce Ca-oxalate, which can bind Fe, Al • Enhance production of chelates, organic acids, siderophores
  • 26. Storage of Adsorbed P P is continuously absorbed by plants. – Soluble Orthophosphate (Harely and Loughman, 1963) – Soluble Polyphosphate (Martin et al., 1983) – Polyphosphate granules (40%) (Chilvers and Harley, 1980)
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  • 30. Mycorrhizal Factors Affecting Nutrients Uptake  Either it is ECM or AM association Only mycorrhizal pathway in ECM Both pathways in AM  Plant & Fungal species  Fungal species effect on activity of pathway  Efficiency with which both partners interact & exchange
  • 31. Factors Affecting on Mycorrhizal Growth Enhance Colonization • Less fertile soil • Natural ecosystem • Coarse texture soil • Slow root growth Decrease colonization • High P conc. • Agronomic practices • Long fallow period • Tillage • Fine texture soil • Rapid root growth
  • 32. Is There Any Negative Effect of Mycorrhiza on Plant? - High C uptake by myc. Unhealthy plant High Myc. growth Very low P in soil
  • 33. (Schroeder and Janos 2004; Smith and Smith 1996; West et al. 1993; Whitbeck 2001) & Morgan et al. 2005 Apply poorly available source of Phosphorous NaHCO3-extractable P levels typically between 5 & 20 mg/kg