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MUSTARD
MUSTARD
MUSTARD
VINUTHA PATIL S
UAHS
• Family: Cruciferae
• Genus: Brassica
• Species: campestris, napus , juncea , rapa ,
nigra , carinata
• Origin: middle east
Emasculation, crossing and selfing
techniques
• Flower buds that open next day are selected
and the remainder of the bud and flowers and
flowering branches are removed. Flowers are
emasculated in the evening and pollinated
and pollinated the next morning.
• The petales as well as stamens of the selected
buds are removed with a pair of tweezers and
the emasculated flowers are bagged.
• Ripe anthers are collected next morning and
pollination is made by dusting pollens from the
ripened anthers over the stigma. After
pollination,the flower are again bagged.
• Flowers to be selfed should be bagged before
they open to avoid natural cross pollinated. Bag
should be of such size as to allow lengthening of
flower.
• Insect also enhance pollination ,leading to cross
pollination.
Breeding objectives:
• Seed yeild
• Earlymaturity
• Resistance to abiotic factores
• Resistance to biotic streess
• Herbicide resistance
• Shattering resistance
• Increase oil content and quality
• Meal quality
Breeding methods
• Introduction- regina from sweden
• Seletion-eg:seeta, krishna , kranti
• Population improvement-Recurrent seletion ,
mass selection
• Heterosis breeding-cms lines- first cms based
hybrid nrc sankar (nrchb506)is the first mori
candia cms based hybrid of indian mustard
• Mutation breeding-eg:regina, RLM198
• Tissue culture technique for production of
homozygous diploids- saline resistance
screening. Induction of mutation in haploids.
• Embryo rescue tecnique for inter specific
crosses.
Transgenic for male sterility
• Transgenic brassica napus-male sterility and
fertility
• TA29-Rnase: branase gene – ribonuclease gene
with TA29(tapetum specific promoter)- male
sterlity
• Barstar gene-inhibitor of ribonuclease encoded
by barnase gene
• Both gene are isolated from bacillius
amylolifaciens
• When barnase and barstar both are present-
plant will be male fertile barstar is dominant
over barnase.
Breeding center:
• Directorate of rapeseed-mustard research
(DRMR)-bharatpur-rajasthan
• IIOR-Hydrabad
Varities:
Pusa agrani, geeta, kranti, jagannadh, jawahar
mustard 2, vasundhara, varuna, vardhan, M-
27, pusa kalyani.
Hybrids:
NRCDR-02 (high yielding indian mustard variety)
NRCHB-506 (1st indian mustard hybrid with high
yeild quality)
• NRCHB-101 (Indian mustard variety for late
sown irrigated condition)
• NRCYS-05-02 (yellow sarson variety suitable
to all yellow sarson growing region of the
country)
THANK
YOU

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Mustard

  • 3. • Family: Cruciferae • Genus: Brassica • Species: campestris, napus , juncea , rapa , nigra , carinata • Origin: middle east
  • 4.
  • 5. Emasculation, crossing and selfing techniques • Flower buds that open next day are selected and the remainder of the bud and flowers and flowering branches are removed. Flowers are emasculated in the evening and pollinated and pollinated the next morning. • The petales as well as stamens of the selected buds are removed with a pair of tweezers and the emasculated flowers are bagged.
  • 6. • Ripe anthers are collected next morning and pollination is made by dusting pollens from the ripened anthers over the stigma. After pollination,the flower are again bagged. • Flowers to be selfed should be bagged before they open to avoid natural cross pollinated. Bag should be of such size as to allow lengthening of flower. • Insect also enhance pollination ,leading to cross pollination.
  • 7. Breeding objectives: • Seed yeild • Earlymaturity • Resistance to abiotic factores • Resistance to biotic streess • Herbicide resistance • Shattering resistance • Increase oil content and quality • Meal quality
  • 8. Breeding methods • Introduction- regina from sweden • Seletion-eg:seeta, krishna , kranti • Population improvement-Recurrent seletion , mass selection • Heterosis breeding-cms lines- first cms based hybrid nrc sankar (nrchb506)is the first mori candia cms based hybrid of indian mustard • Mutation breeding-eg:regina, RLM198
  • 9. • Tissue culture technique for production of homozygous diploids- saline resistance screening. Induction of mutation in haploids. • Embryo rescue tecnique for inter specific crosses.
  • 10. Transgenic for male sterility • Transgenic brassica napus-male sterility and fertility • TA29-Rnase: branase gene – ribonuclease gene with TA29(tapetum specific promoter)- male sterlity • Barstar gene-inhibitor of ribonuclease encoded by barnase gene • Both gene are isolated from bacillius amylolifaciens • When barnase and barstar both are present- plant will be male fertile barstar is dominant over barnase.
  • 11. Breeding center: • Directorate of rapeseed-mustard research (DRMR)-bharatpur-rajasthan • IIOR-Hydrabad
  • 12. Varities: Pusa agrani, geeta, kranti, jagannadh, jawahar mustard 2, vasundhara, varuna, vardhan, M- 27, pusa kalyani. Hybrids: NRCDR-02 (high yielding indian mustard variety) NRCHB-506 (1st indian mustard hybrid with high yeild quality)
  • 13. • NRCHB-101 (Indian mustard variety for late sown irrigated condition) • NRCYS-05-02 (yellow sarson variety suitable to all yellow sarson growing region of the country)

Editor's Notes

  1. MUSTARD
  2. THANK YOU