GPB 311: RICE-Centre of origin, distribution of species, wild relatives and major breeding objectives and procedures for development of varieties and hybrids for improvement yield, adoptability, stability, biotic and abiotic stress tolerance and quality of Rice crop.
GPB 311: RICE-Centre of origin, distribution of species, wild relatives and major breeding objectives and procedures for development of varieties and hybrids for improvement yield, adoptability, stability, biotic and abiotic stress tolerance and quality of Rice crop.
GPB 311: Maize- Centre of origin, distribution of species, wild relatives and major breeding objectives and procedures for development of varieties and hybrids for improvement yield, adoptability, stability, biotic and abiotic stress tolerance and quality of Maize
Study in respect to origin distribution of species –wild relatives- and forms of breeding objectives –major breeding procedure for development of hybrids varieties in wheat
GPB 311: Wheat- Centre of origin, distribution of species, wild relatives and major breeding objectives and procedures for development of varieties and hybrids for improvement yield, adoptability, stability, biotic and abiotic stress tolerance and quality in Wheat
In this slide described the physiology of rice on the basis of breeding purpose. covered information about golden rice and hybrid rice. Also list out the varieties of rice developed in Maharashtra state and by Dr. Punjabrao Deshmukh Agriculture University, Akola.
GPB 311: Maize- Centre of origin, distribution of species, wild relatives and major breeding objectives and procedures for development of varieties and hybrids for improvement yield, adoptability, stability, biotic and abiotic stress tolerance and quality of Maize
Study in respect to origin distribution of species –wild relatives- and forms of breeding objectives –major breeding procedure for development of hybrids varieties in wheat
GPB 311: Wheat- Centre of origin, distribution of species, wild relatives and major breeding objectives and procedures for development of varieties and hybrids for improvement yield, adoptability, stability, biotic and abiotic stress tolerance and quality in Wheat
In this slide described the physiology of rice on the basis of breeding purpose. covered information about golden rice and hybrid rice. Also list out the varieties of rice developed in Maharashtra state and by Dr. Punjabrao Deshmukh Agriculture University, Akola.
RICE
Oryza sativa (2n=24)
Rice is one of the oldest cultivated crops. The two cultivated species of rice are i) Oryza sativa - Asian rice
ii) O. glaberrima - African rice. The three races in cultivated Asian rice are
i) indica
ii) Japonica (Sinica)
iii) Javanica.
Origin of cultivated rice.
The views regarding the origin of rice can be grouped in to two classes viz., a) Polyphyletic origin
b) Monophyletic origin.
i.Polyphyletic: Originated from several species. According to this theory, the two forms of cultivated rice viz., Asian rice O.sativa and African rice O.glaberrima have evolved independently in their respective regions from several species.
Common ancestor
South & South East Asia Tropical Africa Perennial O.rufipogon O.longistaminata Annual O.nivara Weedy annual O.barthii
O.spontanea
O.sativa O.Staffii O.glaberrima
indica japonica javanica
ii. Monophyletic : According to this theory both Asian rice and African rice arose from a common parent (O.perennis). This view is the most accepted one because both Asian rice and African rice are similar except in glume pubescence, ligule size and colour of pericarp which is red in African rice.
O.perennis
O.glaberrima O.sativa
According to polyphyletic origin the present day rice varieties have originated from several species. According to monophyletic origin a single species has given rise to all varieties of cultivated rice. Viz.,
Oryza sativa
Oryza glaberrima
most of the modern rice workers believe that origin of cultivated rice monophyletic. From oryza perennis rose the Asian rice in South East tropical Asia and African rice in the upper valley of Niger River in Africa.
Species in the genus oryza:
According to the latest view the genus oryza include 20 wild species. Out of these two are cultivated diploids viz. O.sativa and O.glaberrima and rest are wild species which include both diploid and tetraploid forms.
GREEN GRAM (MUNG BEAN)
vigna radiata (2n = 22)
It is esteemed as the most wholesome among the pulses, free from the heaviness and tendency to cause flatulence, which is associated with other pulses.
Place of origin : India
Wild relative : Vigna radiata var. sublobata
BLACK GRAM (URAD, ULUNDU)
Vigna mungo (2n = 22, 24)
Origin : India
Putative parents
V. trinerivus / V. sublobata or V.mungo var. sylvestris.
Breeding objectives
1. Evolving medium duration high yielding varieties for dry land cultivation.
Co5 black gram. Suitable for dry land cultivation.
PEARL MILLET
Pennisetum glaucum (2n = 14)
(Cumbu, Bajra, Bulrush millet)
Origin : West Africa.
Taxonomy : The genus pennisetum is having more than 140 species. Stapf (1954) has
divided the genus pennisetum in to five sections viz.,
1. Gymnothrix
2. Eupennisetum
3. Penicillaria
4. Heterostachya
5. Brevivalvula
The cultivated Pennisetum glaucum belongs to the section penicillaria.
Origin and putative parents.
Stapf included 32 species is penicillaria. Of these 32 species found is Africa, six
annuals are considered wild and probable ancestors of the cultivated one. They are
1. Pennisetum perottettii
2. P. molllissimum
3. P. violaceum
4. P. versicolor
5. P. adonense
6. P. gymnothrix
The cultivated species of pennisetum is believed to have originated thro’
hybridization with in these six species.
Wild species utilised in breeding :
The other species in this section is P.purpureum a rhizomatus perennial having
chromosome number 2n = 28
cumbu napier hybrid = BN1
Tetraploid x Diploid - Triploid.
P. squamulatum (2n = 46) - Drought and cold resistant having apomictic line crossed
with P.glaucum to evolve superior cold resistant fodder.
P. orientale : used for transferring apomixis.
P. setaceum P. violaceum : To transfer male sterile genes to P.glaucum
Inter generic crosses :
Buffel grass Cenchrus ciliaris or Pennisetum ciliare utilised to cross with cumbu
for fodder improvement
PRESENT STATUS AND FUTURE STRATEGIES IN COLLECTION OF MAJOR CROPS OF COTTON, ...Dhanuja Kumar
Cotton has played a great role in the global and Indian economies since immemorial time. The antiquity of cotton in the Indian subcontinent has been traced to the 4th millennium BC.
The wild species of Gossypium are important sources of useful traits such as special and superior fibre properties, cytoplasmic male sterility, resistance to biotic and abiotic stresses etc. which can be introgressed into the cultivated species for improvement. Since the variability available in cultivated germplasm is limited and has been exhaustively utilized in breeding programmes, it has become a necessity to collect, conserve and develop basic germplasm materials enriched with rare useful genes.
Conservation is very important in mango, because many species are becoming extinct and many others are threatened and endangered.
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Similar to Presentation on Breeding Techniques of Blackgram (20)
Gene mutations – introduction – definition – a brief history – terminology –
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Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
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Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
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development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
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Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
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1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
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This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
2. INTRODUCTION
BLACKGRAM
• Chromosome no.-2n=22
• Botanical name-Vigna mungo L. Wilczek
• Family-fabaceae
• Centre of origin-India
• Mode of pollination-Self-pollination
• Out-crossing percentage-0.5%
• Related wild species-Vigna angularis
Blackgram is the fourth important pulse crop in india covering an
area of about 3 million hectares ,but producing only 1.2 million
tonnes.
It is mostly grown in madhya pradesh,maharashtra,tamil nadu,uttar
pradesh,and rajasthan during kharif and in andhra pradesh and
west bengal in rabi.
3. PROGENITOR
According to Verdcourt(1971)one wild species Vigna
radiata var. sulobata has given rise to two cultigen,
namely Vigna mungo(blackgram) and Vigna
radiata(greengram).However,Chandel(1984) reported
that V.mungo domesticated from V.mungo var.
silvestris instead of V.radiata var. sublobata
Biochemical evidence also indicate similarity between
mungbean and its wild progenitor V.radiata var.
sublobata and that between urdbean and wild
ancestral from V.mungo var. silvestris as demonstrated
by the presence of glutamyl-S-methyl-cysteins.
4. TAXONOMY
Vigna mungo var. mungo Vigna mungo var. silvestris
Leaflets entire ,occassionally 2-3
shallow broad lobed or highly dissected
with deep lobes,
Plants and pods hairy
Seeds usually globular ,dull, green or
blackish mottled
Seed coat cellular structure
hexagonal,cup shaped
Hilum deeply concaved ,raised with
mounds ,arilate
Leaflets large,the terminal 7.0*5.0cm
Infloroscence 6-8 flowered
Pods 4-6 borne horizontally , spreading,
deflexed ,black 10-14 seeded
,septate,seeds sub brownish
Hilum deeply furrowed concave
7. BOTANY
Blackgram is an erect or sub-erect ,diffusely branched
herbaceous annual.Infloroscence ia an axillary or terminal
raceme with 10-20 flowers crowded on long
peduncle.Flowering is indeterminate in habit.
A flower is hermaphrodite ,consists of 5 sepals ,5 petals (1
standard,2 wings, and 2 keels),10 stamens which are in
diadelphous(9+1)condition and single carpel.
Anthers dehiscing during night(9pm -3am)and flowers open
from 6-8am and remains open up to 11am .So that self-
fertilisation is the rule(pollination occurs in bud stage).
The gynoecium is monocarpellary with a superior unilocular
ovary having marginal placentation with 8-12 ovules.The style
is twisted below the stigmatic surface and stigma is hairy.
8. CYTOLOGY
The somatic chromosome no. in urdbean is
2n=2x=22.The average chromosome length is about
1.70cm.Majority of the chomosomes are metacentric
i.e.,the arm-ratio is 1.00-1.75
There are no sub-terminal/acrocentric chromosomes
and 0.2 chromosomes are reported to have secondary
constriction (Gupta and Sharma ,1991).Like V.radiata
,V.mungo has been successfully crossed with
V.sublobata,V.trilobata,V.umbellata and V.angularis.
Mungbean *urdbean crosses are easily made while the
reciprocal cross is possible only through embryo rescue
technique(Gosal and Bajaj,1983).
9. QUALITATIVE GENETICS
• Standard gene symbols are not available as yet.Therefore
,characterwise brief information is given here based on compilation
by Singh(1991).
PLANT TYPE:erect habit ,incompletely dominant over spreading
over spreading habit ,single gene pair.
LEAF TYPE:1)monogenic,ovate dominant over lanceolate.2)hastate
leaf shape dominant over ovate and controlled by duplicate
dominant genes.
POD-PUBESCENCE:pubescent pod monogenically dominant over
non-pubescent type.
POD-COLOUR:usually monogenic and black colour dominant over
straw or brown.
SEEDCOAT COLOUR:1)monogenic,green dominant over
brown.2)black coat ,monogenic and dominant over
brown,.3)shining seed ,monogenic,dominant over dull.
11. • Evolving short duration varieties suitable for rice fallow
conditions
• Breeding for better quality:
24% protein. There are lines having 27% protein. These
can be utilized.Quality of black gram is determined by
a) Protein content
b) Methionine content 1.17%
c) cooking quality - Time
d) % of hard seeds.
e) Dal recovery 70%
13. BREEDING PROCEDURE
1. Introduction :
2. Pure line selection :
3. Hybridization and selection
a) Intervarital
b) Inter specific :
Vigna mungo x V.mungo var.sylvestris - Pantnagar.
YMV resistant line are obtained,but pod shatters. More number of
back crosses suggested.
Vigna mungo x V.radiata for increasing pod length, digestibility.
Sterility is the main problem. Few plants obtained revert back to
parental form.
15. IDEAL PLANT TYPE
• For irrigated and Rice fallows:
Determinate type, short duration, high dry matter
producing with 30cm plant ht.
Photo insensitive.
• For rainfed condition :
Semi determinate with pod setting from base of the
main stem; higher pod length and more number
of seeds / pod.
16. RESISTANCE TO DISEASES
• The sources of resistance to these diseases have been
compiled by Singh(1981,1991)and Sharma(1996).A selective
list of donors from these sources is as follows:
MUNGBEAN
YELLOW MOSAIC
VIRUS
UPA-1,UPA-2,Pant U 19,Pant U-
26,Pant U 30,UAH 2
MOSAIC MOTTLE
VIRUS
T-65,T-67
CERCOSPORA LEAF
SPOT
3529,4387,Pant U 19,IC 1008
POWDERY MILDEW UA 2A,UAH 2B,UG 117,HPU 1, HPU
2,HPU 5,P 47,P 163,P 178,Pant U 19
ROOT AND STEM
ROT
3115
BACTERIAL BLIGHT Krishna,13/10,13/2,1369/4,PLU 443
17. RESISTANCE TO INSECT PEST
• White fly, aphids ,leaf eating larvae ,and pod-
borers may cause considerable damage to
blackgram.A few sources of resistance have been
reported by Chhabra et.al.(1984).These are LU
178,LU 190,as resistant to whitefly and LU 126,LU
178,and LU 196 as resistant to jassids.
• These should be used in the crossing programmes
and the segreggating materials be exposed and
screened against these insects,preferably in field
conditions under adequate pressure of thbe
insects.
18. BREEDING CENTRES
• ICRISAT - Hyderabad
• ICARDA - (International Crops for Agricultural
Research in Dry land Areas) – Syria
• AVRDC - (Asian Vegetable Research and
Development Centre)
• IIPR - (Indian Institute of Pulse Research),
Kanpur
19. NATIONAL PROGRAMME
• Urdbean is one of the pulses under All India
Coordinated Pulses Improvement Project
commisioned in 1965-66 and subsequently
upgraded to project Directorate headquarters
at kanpur in 1984.Now,it is known as INDIAN
INSTITUTE OF PULSES RESEARCH(IIPR).
20. CONCLUSION
• Blackgram originated in india and grown in considerable area in the states
of A.P,bihar,M.P,maharashtra,U.P,and W.B.Several high yielding and
disease resistant cultivars developed through routine breeding approches
have been released.Inspite of this,production ,area,and productivity have
remained virtually stagnant around 1.2 million tonnes,3 million hectares
and 400kg/ha,respectively over the last 20 years in india.
• The situation is unlikely devoid of excessive vegetative growth and with
higher no. of seeds/pod are developed.
• To some extent,seed/pod can be improved from mungbean*urdbean
hybridization programme on a large scale.
• Biotechnological approaches as yet have only a limited applicability in
urdbean due to failure of regeneration from protoplast.