SlideShare a Scribd company logo
Ghulam Destgeer
Particle Separation and Chemical Gradient Control
via
Focused Travelling Surface Acoustic Waves (F-TSAW)
Flow Control Laboratory, Department of Mechanical Engineering
2013.06.10
2
Contents
• Introduction
• Theory
• Device design and fabrication
• Experimental setup
• Results
• Summary
Introduction
Surface acoustic wave
4
Particle separation
• The isolation and separation of micro
particulate materials in a continuous
flow are required for chemical
syntheses and biological analyses.
• The separation and sorting of cells are
critical in a variety of biomedical
applications including:
i. Diagnostics
ii. Therapeutics
iii. Cell biology
<Lee et al., 2010, Lab Chip> <Daniel et al., 2010, Anal Bioanal Chem>
Huang’s group
Sung’s group
5
Particle manipulation by SSAW
• Particle separation:
– Particle diameter: 0.87μm (Red), 4.16μm (Green)
• Experimental parameters:
– Frequency: 12.6MHz
– Power: 15-22dBm (30-160mW)
– Flow rate: 0.6-2μl/min
<Shi et al., 2009, Lab Chip>
6
Chemical gradient control
• Most methods are capable of
generating linear chemical
gradient profiles in a static
manner.
• Generating pulsatile chemical
gradients in microfluidic devices
has important implications for
the characterization of dynamic
biological and chemical
processes.
• Dynamic temporal control of
chemical gradients is required.
<Ahmed et al., 2013, Lab Chip> <Daniel et al., 2006, Anal. Chem.> <Seidi et al., 2011, Biomicrofluidics>
7
Chemical gradient control by oscillating bubbles
• Chemical solutions:
– Dextran-FITC (stimulant)
– Phosphate buffered saline(buffer)
• Input voltage and frequency:
– 12-16Vpp and 30kHz
<Ahmed et al., 2013, Lab Chip>
8
Objective
• (a) Device schematic (b) Particle separation
• (c) Chemical gradient control and uniform micromixing
• (d) F-TSAW amplitude (e) Fabricated device
Theory
10
Acoustic radiation force on compressible spheres
For TSAW:
<Yosioka & Kawasima, 1955, Acoustica>
𝐸 𝑎𝑐 = (4π𝑓𝑢)2
𝜌 𝑓 𝐴 2
=
2(4π𝑓𝑢)2
𝑘2
= 8(𝑐𝑢)2𝐴 2
=
2 𝐸 𝑎𝑐
𝑘2 𝜌 𝑓
where, 𝐸 𝑎𝑐 is acoustic energy density, 𝑢 is SAW amplitude, 𝑘 = 2π
λ is
wavenumber, 𝑐 is speed of sound on wafer surface, 𝑓 is the frequency of SAW, Pin
is the input power and V is the input voltage.
where, 𝛼 =
𝜌 𝑓
𝜌 𝑝
, 𝛽 =
𝑐 𝑓
𝑐 𝑝
, R is radius of μ-particles & A is complex amplitude
of the velocity potential.
𝐹 𝑇𝑆𝐴𝑊 = 2𝜋𝜌 𝑓 𝐴 2(𝑘𝑅)6φ 𝑇𝑆𝐴𝑊
φ 𝑇𝑆𝐴𝑊 =
1 −
𝛼 2 + 𝛼 𝛽2
3
2
+
2 1 − 𝛼 2
9
(2 + 𝛼)2
TSAW
Flow
𝐹 𝑇𝑆𝐴𝑊 ~ 𝑉2
𝑓6
𝑅6
~ Pin 𝑓6
𝑅6
11
F-TSAW amplitude
• Acoustic wave amplitude is estimated as:
• Acoustic wave amplitude qualitative measure:
𝑢(𝑥, 𝑧) ≈
1
𝑍1/4
−∞
+∞
𝐺 𝑡 exp 𝑗 𝑡4
+𝑍′
𝑡2
+𝑋′
𝑡 𝑑𝑡
𝑍′
=
(0.145𝑍 − 𝑅𝑘0/2)
4.98𝑍
𝑋′ =
−𝑋
4
4.98𝑍
𝑡 =
4
4.98𝑍𝐾1 𝑍 = 𝑘0 𝑧 𝑋 = 𝑘0 𝑥 𝐾1 = 𝑘1/𝑘0
<Fang and Zhang, 1989, IEEE Transactions on Ultrasonic> <Wu et al., 2005, IEEE Transactions on Ultrasonic>
Wu et al. 2005 Calculated
θ
R
A A’
12
F-TSAW amplitude
5R 5R 5R
5R 5R 5R
5R 5R 5R
AmplitudeAmplitudeAmplitude
AmplitudeAmplitudeAmplitude
AmplitudeAmplitudeAmplitude
13
F-TSAW amplitude
Frequency(MHz)
14
SAW amplitude calculation
F 𝑇𝑆𝐴𝑊~ (Eac/k2) (kR)6φ 𝑇𝑆𝐴𝑊
E 𝑎𝑐~ u2 f2 ρ
Energy density (Eac) – J/m3
SAW displacement (u) – nm
Frequency (f) – MHz
Density (ρ) – kg/m3
Wave number (k) – (μm)-1
Particle radium (R) – (μm)
Constant (φ)
Contour plots of SAW displacement square (u2) – m2
Top – f =133.3MHz
Bottom – f = 40.0MHz
x
z
Device design and fabrication
16
F-TSAW device design
• Two salient features: (i) unidirectional (ii) focused
• Interdigitated transducer(IDT): Two interlocking
comb-shaped metallic electrodes on top of a
piezoelectric substrate.
• Frequency of applied AC signal = frequency of
SAW (fSAW)
– fSAW = c/λ, c is speed of sound in the piezoelectric
substrate
Maximum energy is
transmitted in the
forward direction.
Very little energy is transmitted
in the backward direction.
SAW
λ
λ/8
λ/43λ/16
SAW
Unidirectional transducer
λλ/4
SAW SAW
IDT
F-TSAW amplitude by
a focusing transducer
17
Fabrication of micro-chip
18
Microfluidic channels
150µm 500µm200µm
1st 2nd 3rd
19
Focused IDTs
40MHz 133.3MHz
1st 2nd
Experimental setup
21
Experiment schematic
Signal generator, N5181A [3GHz]
μ-pump, neMESYS
Microscope, BX51
Camera, DP26
Power amplifier, ZHL-1-2W
DC power supply, E3634A
Micro
Chip
PDMSLiNbO3
Au electrodes
22
Experimental setup
Power supply
Amplifier
Signal generator
Microscope
Microchip
Micropump
Display screen
Oscilloscope
Camera
Results
24
Experimental parameters
PARAMETERS Device #1 Device #2 Device #3 Device #4
Frequency (MHz) 40 133.3 133.3 133.3
Input power 0.25µW 0.45mW 0.07mW ---
After amplification 0.175mW 275mW 63mW 60–200mW
Radius of FUT (mm) 6 4 4 4
Distance from FUT
to microchannel
1.25R 2.5R 2.5R 2.5R
µ-channel
cross section (µm×µm)
150×110 150×45 200×40 500×90
Particles diameter (µm) 30 and 10 10 and 3 10 and 3 ---
Fluid/media DI water DI water DI water
DI water,
rhodamine
Total flow rate (µl/hr) 50 150 100 100
Average velocity (mm/s) 0.84 6.17 3.5 0.6
Function
Particle
Separation
Particle
Separation
Particle
Separation
Gradient
Generation
Names CAPS-1 CAPS-2 CAPS-3 CAGG
*CAPS: Cross-type Acoustic Particle Separator
*CAGG: Cross-type Acoustic Gradient Generator
25
CAPS-1: Particle trajectory and separation
• Experimental conditions:
– Frequency (f): 40MHz (Low)
– Input power: 725µW
– Flow rate (Q): 50μl/h (0.84mm/s)
– μ-channel cross-section: 150x110μm
– μ-particles diameter: 10, 30μm
• Equation of particle motion:
• Acoustic radiation force:
• Stokes drag force:
• Particle trajectory:
– Left figure: Theoretical
– Center figure: Experimental
• Particle separation on right
𝑚 𝑧 = 𝐹 𝑇𝑆𝐴𝑊 − 𝐹 𝐷
𝐹 𝑇𝑆𝐴𝑊 = 4π𝐸 𝑎𝑐 𝑘4 𝑅6φ 𝑇𝑆𝐴𝑊
𝐹 𝐷 = 6πη𝑅 𝑧
26
CAPS-2: Particle trajectory and separation
• Experimental conditions:
– Frequency (f): 133.3MHz (High)
– Input power: 1.36W
– Flow rate (Q): 150μl/h
(6.17mm/s)
– μ-channel cross-section:
150x45μm
– μ-particles diameter: 10, 3μm
• (a) Schematic diagram of a
PDMS microchannel.
• (b-c) Once the TSAW was
turned ON, a distinct
separation distance could be
observed.
• (d) Trajectory followed by a 10
µm particle influenced by
acoustic streaming.
27
CAPS-3: Particle trajectory and separation
• Experimental conditions:
– Frequency: 133.3MHz
– Input power: 225mW
– μ-channel cross-section:
• h x w: 40 x 200 μm
– Flow rate (Q):
• Sample+ Sheath: 25μl/h + 75μl/h = 100μl/h
• Average speed: 3.5mm/s
– μ-particles diameter: 3μm and 10μm
• Left: TSAW OFF, all the particles flowing
together with the laminar flow.
• Right: TSAW ON, larger particles are
pushed towards the opposite wall
resulting in separation
28
Particle separation efficiency
• (a) TSAW OFF: all of the particles are
collected at the same outlet
• (b) TSAW ON: 3µm particles are
collected at same outlet whereas
almost 100% of the 10µm particles
passed through a separate outlet.
(a) (b)
29
CAPS-3: Particle deflection vs. input power
• Flow rate is kept constant:
– Sheath + Sample = 80 + 20 = 100 µlh-1
30
CAPS-3: Deflection vs. input power and flow rate
• For particles with diameter 10µm:
31
CAPS-3: Deflection (µm) vs. Input Power (mW)
32
CAGG
• Acoustic streaming flow induced
via F-TSAW
• Flow is traced by 1µm polymer
microspheres dispersed in DI
water.
• On smaller particles, drag force is
dominant compared to acoustic
radiation force.
• Three microchannels 150µm x
45µm, 200µm x 40µm and 500µm
x 90µm from left to right,
respectively, are tested.
• Microchannel 500µm x 90µm can
produce strong and large vortices
appropriate for mixing and
gradient control.
F-TSAW
F-TSAW
33
Chemical gradient control and micromixing
• Acoustic streaming flow
– Generate chemical gradient
– Uniformly mix fluids.
• Microchannel
– w×h: 500µm×90µm
• Flow rate: 100µl/h (0.6mm/s)
– Fluid 1: rhodamine: 50µl/h
– Fluid 2: DI water: 50 µl/h
• Power input
– Gradient control: 60–200mW (18–
23dBm)
– Uniform mixing: 800mW (29dBm)
34
Chemical gradient control and micromixing
35
Summary
• Four types of devices are tested:
– First three are Cross-type Acoustic Particle Separator (CAPS)
– Fourth is Cross-type Acoustic Gradient Generator (CAGG)
• A single micro-chip is capable to be used as CAPS or CAGG
• Particles are successfully separated with efficiency close to
100%:
– 10μm particles from 3μm and 30μm particles from 10μm
• Particle deflection is plotted against input power which
shows:
– 3μm, 7μm and 10μm are separated
• Low amplitude and high frequency (40 and 133.3MHz) waves
are used.
• Chemical gradient control and uniform mixing is also shown
using F-TSAW without trapping any micro-bubble.
THANK YOU FOR YOUR ATTENTION!!!

More Related Content

What's hot

Surface acoustic wave (saw) radio transponders
Surface acoustic wave (saw) radio transpondersSurface acoustic wave (saw) radio transponders
Surface acoustic wave (saw) radio transpondersRehan Fazal
 
Surface acoustic wave (saw) based sensors
Surface acoustic wave (saw) based sensorsSurface acoustic wave (saw) based sensors
Surface acoustic wave (saw) based sensorssjykmuch
 
Surface and bulk acoustic devices
Surface  and  bulk acoustic   devicesSurface  and  bulk acoustic   devices
Surface and bulk acoustic devices
jenyjose3
 
Magnetostriction and application of ultrasonic waves
Magnetostriction and application of ultrasonic wavesMagnetostriction and application of ultrasonic waves
Magnetostriction and application of ultrasonic waves
PreethiSureshkumar1
 
Acoustic sensors
Acoustic sensorsAcoustic sensors
Acoustic sensors
Shankar Sahni
 
Aaron
AaronAaron
Aaron
Naan Illa
 
Guided Wave Ultrasound - Principles and Apllications
Guided Wave Ultrasound - Principles and ApllicationsGuided Wave Ultrasound - Principles and Apllications
Guided Wave Ultrasound - Principles and Apllications
subash_j
 
ultrasonics ppt
ultrasonics pptultrasonics ppt
ultrasonics ppt
2461998
 
Ultrasonic generation-and-its-application
Ultrasonic generation-and-its-applicationUltrasonic generation-and-its-application
Ultrasonic generation-and-its-application
SYED AHMED SHAH
 
Life detection using microwave L band
Life detection using microwave L bandLife detection using microwave L band
Life detection using microwave L band
shiva kumar cheruku
 
IRJET- Distance Measurement with the Help of Ultrasonic Sensor
IRJET-  	  Distance Measurement with the Help of Ultrasonic SensorIRJET-  	  Distance Measurement with the Help of Ultrasonic Sensor
IRJET- Distance Measurement with the Help of Ultrasonic Sensor
IRJET Journal
 
Ultrasonic testing 1766001
Ultrasonic testing 1766001Ultrasonic testing 1766001
Ultrasonic testing 1766001
Melwyn Mayers
 
Life detection system
Life detection system Life detection system
Life detection system
princep12
 
Life detection system
Life detection systemLife detection system
Life detection system
Satyam Srivastava
 
ultrasonic
ultrasonicultrasonic
ultrasonic
Abhishek Choksi
 
IRJET- Wave Ultrasonic Testing and how to Improve its Characteristics by Vary...
IRJET- Wave Ultrasonic Testing and how to Improve its Characteristics by Vary...IRJET- Wave Ultrasonic Testing and how to Improve its Characteristics by Vary...
IRJET- Wave Ultrasonic Testing and how to Improve its Characteristics by Vary...
IRJET Journal
 
Acoustic Emission (AE) Testing
Acoustic Emission (AE) TestingAcoustic Emission (AE) Testing
Acoustic Emission (AE) Testing
Arahvinth Krishnamoorthy
 
project report
project report project report
Life detection system
Life detection systemLife detection system
Life detection system
sneha manhas
 
Ultrasonic
UltrasonicUltrasonic
Ultrasonic
maharshi solanki
 

What's hot (20)

Surface acoustic wave (saw) radio transponders
Surface acoustic wave (saw) radio transpondersSurface acoustic wave (saw) radio transponders
Surface acoustic wave (saw) radio transponders
 
Surface acoustic wave (saw) based sensors
Surface acoustic wave (saw) based sensorsSurface acoustic wave (saw) based sensors
Surface acoustic wave (saw) based sensors
 
Surface and bulk acoustic devices
Surface  and  bulk acoustic   devicesSurface  and  bulk acoustic   devices
Surface and bulk acoustic devices
 
Magnetostriction and application of ultrasonic waves
Magnetostriction and application of ultrasonic wavesMagnetostriction and application of ultrasonic waves
Magnetostriction and application of ultrasonic waves
 
Acoustic sensors
Acoustic sensorsAcoustic sensors
Acoustic sensors
 
Aaron
AaronAaron
Aaron
 
Guided Wave Ultrasound - Principles and Apllications
Guided Wave Ultrasound - Principles and ApllicationsGuided Wave Ultrasound - Principles and Apllications
Guided Wave Ultrasound - Principles and Apllications
 
ultrasonics ppt
ultrasonics pptultrasonics ppt
ultrasonics ppt
 
Ultrasonic generation-and-its-application
Ultrasonic generation-and-its-applicationUltrasonic generation-and-its-application
Ultrasonic generation-and-its-application
 
Life detection using microwave L band
Life detection using microwave L bandLife detection using microwave L band
Life detection using microwave L band
 
IRJET- Distance Measurement with the Help of Ultrasonic Sensor
IRJET-  	  Distance Measurement with the Help of Ultrasonic SensorIRJET-  	  Distance Measurement with the Help of Ultrasonic Sensor
IRJET- Distance Measurement with the Help of Ultrasonic Sensor
 
Ultrasonic testing 1766001
Ultrasonic testing 1766001Ultrasonic testing 1766001
Ultrasonic testing 1766001
 
Life detection system
Life detection system Life detection system
Life detection system
 
Life detection system
Life detection systemLife detection system
Life detection system
 
ultrasonic
ultrasonicultrasonic
ultrasonic
 
IRJET- Wave Ultrasonic Testing and how to Improve its Characteristics by Vary...
IRJET- Wave Ultrasonic Testing and how to Improve its Characteristics by Vary...IRJET- Wave Ultrasonic Testing and how to Improve its Characteristics by Vary...
IRJET- Wave Ultrasonic Testing and how to Improve its Characteristics by Vary...
 
Acoustic Emission (AE) Testing
Acoustic Emission (AE) TestingAcoustic Emission (AE) Testing
Acoustic Emission (AE) Testing
 
project report
project report project report
project report
 
Life detection system
Life detection systemLife detection system
Life detection system
 
Ultrasonic
UltrasonicUltrasonic
Ultrasonic
 

Viewers also liked

Zahn_poster_4_19
Zahn_poster_4_19Zahn_poster_4_19
Zahn_poster_4_19Elaine Mau
 
Separation of Macromolecules by Their Size: The Mean Span Dimension
Separation of Macromolecules by Their Size: The Mean Span DimensionSeparation of Macromolecules by Their Size: The Mean Span Dimension
Separation of Macromolecules by Their Size: The Mean Span Dimension
cypztm
 
Lectureon liberation separation extraction
Lectureon liberation separation extractionLectureon liberation separation extraction
Lectureon liberation separation extractionPublicaTUDO
 
Gravity slideshare
Gravity slideshareGravity slideshare
Gravity slideshareMegnlish
 
Types of separation notes
Types of separation notesTypes of separation notes
Types of separation notes
Megnlish
 
Separation techniques
Separation techniquesSeparation techniques
Separation techniques
Jamie Ayers
 
14 stokes law and grit separators
14 stokes law and grit separators14 stokes law and grit separators
14 stokes law and grit separators
Akepati S. Reddy
 
Size exclusion chromatography
Size exclusion chromatographySize exclusion chromatography
Size exclusion chromatography
Endler Marcel Borges
 

Viewers also liked (8)

Zahn_poster_4_19
Zahn_poster_4_19Zahn_poster_4_19
Zahn_poster_4_19
 
Separation of Macromolecules by Their Size: The Mean Span Dimension
Separation of Macromolecules by Their Size: The Mean Span DimensionSeparation of Macromolecules by Their Size: The Mean Span Dimension
Separation of Macromolecules by Their Size: The Mean Span Dimension
 
Lectureon liberation separation extraction
Lectureon liberation separation extractionLectureon liberation separation extraction
Lectureon liberation separation extraction
 
Gravity slideshare
Gravity slideshareGravity slideshare
Gravity slideshare
 
Types of separation notes
Types of separation notesTypes of separation notes
Types of separation notes
 
Separation techniques
Separation techniquesSeparation techniques
Separation techniques
 
14 stokes law and grit separators
14 stokes law and grit separators14 stokes law and grit separators
14 stokes law and grit separators
 
Size exclusion chromatography
Size exclusion chromatographySize exclusion chromatography
Size exclusion chromatography
 

Similar to MS Thesis Defense Presentation

Preliminary Exam Presentation
Preliminary Exam PresentationPreliminary Exam Presentation
Preliminary Exam Presentation
ggarner
 
Future accelerator scenarios
Future accelerator scenariosFuture accelerator scenarios
Future accelerator scenarios
ESS BILBAO
 
VISHAV snp.pptx
VISHAV snp.pptxVISHAV snp.pptx
VISHAV snp.pptx
NeerajBhatt62
 
Color flow medical cardiac ultrasound
Color flow medical cardiac ultrasoundColor flow medical cardiac ultrasound
Color flow medical cardiac ultrasound
Larry Miller PhD
 
First results from the full-scale prototype for the Fluorescence detector Arr...
First results from the full-scale prototype for the Fluorescence detector Arr...First results from the full-scale prototype for the Fluorescence detector Arr...
First results from the full-scale prototype for the Fluorescence detector Arr...
Toshihiro FUJII
 
X ray generator basic
X ray generator basicX ray generator basic
X ray generator basic
Md. Serajul islam bablu
 
Basic radio Principles, Electromagnetic Spectrum
Basic radio Principles, Electromagnetic SpectrumBasic radio Principles, Electromagnetic Spectrum
Basic radio Principles, Electromagnetic Spectrum
SubhashMSubhash
 
Magnetic Drug Targeting, James Ritter, PhD
Magnetic Drug Targeting, James Ritter, PhDMagnetic Drug Targeting, James Ritter, PhD
Magnetic Drug Targeting, James Ritter, PhDSCTR Institute
 
Emi test introduction 2017 18
Emi test introduction 2017 18Emi test introduction 2017 18
Emi test introduction 2017 18
rajeshvbe
 
Polymer Waveguide Based Optical Interconnects for High-Speed On-Board Communi...
Polymer Waveguide Based Optical Interconnects for High-Speed On-Board Communi...Polymer Waveguide Based Optical Interconnects for High-Speed On-Board Communi...
Polymer Waveguide Based Optical Interconnects for High-Speed On-Board Communi...
Jian Chen
 
John WM2015 Poster R_0_Final
John WM2015 Poster R_0_FinalJohn WM2015 Poster R_0_Final
John WM2015 Poster R_0_FinalJohn Conley
 
Channel Models for Massive MIMO
Channel Models for Massive MIMOChannel Models for Massive MIMO
Channel Models for Massive MIMO
CPqD
 
Design of ultrasound transducer
Design of ultrasound transducer Design of ultrasound transducer
Design of ultrasound transducer
SudarshanKanse1
 
(May 9, 2024) Enhanced Ultrafast Vector Flow Imaging (VFI) Using Multi-Angle ...
(May 9, 2024) Enhanced Ultrafast Vector Flow Imaging (VFI) Using Multi-Angle ...(May 9, 2024) Enhanced Ultrafast Vector Flow Imaging (VFI) Using Multi-Angle ...
(May 9, 2024) Enhanced Ultrafast Vector Flow Imaging (VFI) Using Multi-Angle ...
Scintica Instrumentation
 
Ldb Convergenze Parallele_sorba_01
Ldb Convergenze Parallele_sorba_01Ldb Convergenze Parallele_sorba_01
Ldb Convergenze Parallele_sorba_01laboratoridalbasso
 
Solve vibration problems using signal processing techniques: a preliminary study
Solve vibration problems using signal processing techniques: a preliminary studySolve vibration problems using signal processing techniques: a preliminary study
Solve vibration problems using signal processing techniques: a preliminary study
National Chung Hsing University
 
méthode electrique et methode magnétique dans la prospection géophysique.ppt
méthode electrique et methode magnétique dans la prospection géophysique.pptméthode electrique et methode magnétique dans la prospection géophysique.ppt
méthode electrique et methode magnétique dans la prospection géophysique.ppt
yassinetiaret
 
EDS softwares INCA and EDAX_EM forum_Yina Guo_May 2016
EDS softwares INCA and EDAX_EM forum_Yina Guo_May 2016EDS softwares INCA and EDAX_EM forum_Yina Guo_May 2016
EDS softwares INCA and EDAX_EM forum_Yina Guo_May 2016
YinaGuo
 

Similar to MS Thesis Defense Presentation (20)

Preliminary Exam Presentation
Preliminary Exam PresentationPreliminary Exam Presentation
Preliminary Exam Presentation
 
Future accelerator scenarios
Future accelerator scenariosFuture accelerator scenarios
Future accelerator scenarios
 
Laser drivenplasma
Laser drivenplasmaLaser drivenplasma
Laser drivenplasma
 
VISHAV snp.pptx
VISHAV snp.pptxVISHAV snp.pptx
VISHAV snp.pptx
 
Color flow medical cardiac ultrasound
Color flow medical cardiac ultrasoundColor flow medical cardiac ultrasound
Color flow medical cardiac ultrasound
 
First results from the full-scale prototype for the Fluorescence detector Arr...
First results from the full-scale prototype for the Fluorescence detector Arr...First results from the full-scale prototype for the Fluorescence detector Arr...
First results from the full-scale prototype for the Fluorescence detector Arr...
 
SENSING WITH CHAOS
SENSING WITH CHAOSSENSING WITH CHAOS
SENSING WITH CHAOS
 
X ray generator basic
X ray generator basicX ray generator basic
X ray generator basic
 
Basic radio Principles, Electromagnetic Spectrum
Basic radio Principles, Electromagnetic SpectrumBasic radio Principles, Electromagnetic Spectrum
Basic radio Principles, Electromagnetic Spectrum
 
Magnetic Drug Targeting, James Ritter, PhD
Magnetic Drug Targeting, James Ritter, PhDMagnetic Drug Targeting, James Ritter, PhD
Magnetic Drug Targeting, James Ritter, PhD
 
Emi test introduction 2017 18
Emi test introduction 2017 18Emi test introduction 2017 18
Emi test introduction 2017 18
 
Polymer Waveguide Based Optical Interconnects for High-Speed On-Board Communi...
Polymer Waveguide Based Optical Interconnects for High-Speed On-Board Communi...Polymer Waveguide Based Optical Interconnects for High-Speed On-Board Communi...
Polymer Waveguide Based Optical Interconnects for High-Speed On-Board Communi...
 
John WM2015 Poster R_0_Final
John WM2015 Poster R_0_FinalJohn WM2015 Poster R_0_Final
John WM2015 Poster R_0_Final
 
Channel Models for Massive MIMO
Channel Models for Massive MIMOChannel Models for Massive MIMO
Channel Models for Massive MIMO
 
Design of ultrasound transducer
Design of ultrasound transducer Design of ultrasound transducer
Design of ultrasound transducer
 
(May 9, 2024) Enhanced Ultrafast Vector Flow Imaging (VFI) Using Multi-Angle ...
(May 9, 2024) Enhanced Ultrafast Vector Flow Imaging (VFI) Using Multi-Angle ...(May 9, 2024) Enhanced Ultrafast Vector Flow Imaging (VFI) Using Multi-Angle ...
(May 9, 2024) Enhanced Ultrafast Vector Flow Imaging (VFI) Using Multi-Angle ...
 
Ldb Convergenze Parallele_sorba_01
Ldb Convergenze Parallele_sorba_01Ldb Convergenze Parallele_sorba_01
Ldb Convergenze Parallele_sorba_01
 
Solve vibration problems using signal processing techniques: a preliminary study
Solve vibration problems using signal processing techniques: a preliminary studySolve vibration problems using signal processing techniques: a preliminary study
Solve vibration problems using signal processing techniques: a preliminary study
 
méthode electrique et methode magnétique dans la prospection géophysique.ppt
méthode electrique et methode magnétique dans la prospection géophysique.pptméthode electrique et methode magnétique dans la prospection géophysique.ppt
méthode electrique et methode magnétique dans la prospection géophysique.ppt
 
EDS softwares INCA and EDAX_EM forum_Yina Guo_May 2016
EDS softwares INCA and EDAX_EM forum_Yina Guo_May 2016EDS softwares INCA and EDAX_EM forum_Yina Guo_May 2016
EDS softwares INCA and EDAX_EM forum_Yina Guo_May 2016
 

Recently uploaded

The geography of Taylor Swift - some ideas
The geography of Taylor Swift - some ideasThe geography of Taylor Swift - some ideas
The geography of Taylor Swift - some ideas
GeoBlogs
 
2024.06.01 Introducing a competency framework for languag learning materials ...
2024.06.01 Introducing a competency framework for languag learning materials ...2024.06.01 Introducing a competency framework for languag learning materials ...
2024.06.01 Introducing a competency framework for languag learning materials ...
Sandy Millin
 
The Challenger.pdf DNHS Official Publication
The Challenger.pdf DNHS Official PublicationThe Challenger.pdf DNHS Official Publication
The Challenger.pdf DNHS Official Publication
Delapenabediema
 
The approach at University of Liverpool.pptx
The approach at University of Liverpool.pptxThe approach at University of Liverpool.pptx
The approach at University of Liverpool.pptx
Jisc
 
Chapter 3 - Islamic Banking Products and Services.pptx
Chapter 3 - Islamic Banking Products and Services.pptxChapter 3 - Islamic Banking Products and Services.pptx
Chapter 3 - Islamic Banking Products and Services.pptx
Mohd Adib Abd Muin, Senior Lecturer at Universiti Utara Malaysia
 
Synthetic Fiber Construction in lab .pptx
Synthetic Fiber Construction in lab .pptxSynthetic Fiber Construction in lab .pptx
Synthetic Fiber Construction in lab .pptx
Pavel ( NSTU)
 
Mule 4.6 & Java 17 Upgrade | MuleSoft Mysore Meetup #46
Mule 4.6 & Java 17 Upgrade | MuleSoft Mysore Meetup #46Mule 4.6 & Java 17 Upgrade | MuleSoft Mysore Meetup #46
Mule 4.6 & Java 17 Upgrade | MuleSoft Mysore Meetup #46
MysoreMuleSoftMeetup
 
Polish students' mobility in the Czech Republic
Polish students' mobility in the Czech RepublicPolish students' mobility in the Czech Republic
Polish students' mobility in the Czech Republic
Anna Sz.
 
Francesca Gottschalk - How can education support child empowerment.pptx
Francesca Gottschalk - How can education support child empowerment.pptxFrancesca Gottschalk - How can education support child empowerment.pptx
Francesca Gottschalk - How can education support child empowerment.pptx
EduSkills OECD
 
Palestine last event orientationfvgnh .pptx
Palestine last event orientationfvgnh .pptxPalestine last event orientationfvgnh .pptx
Palestine last event orientationfvgnh .pptx
RaedMohamed3
 
Home assignment II on Spectroscopy 2024 Answers.pdf
Home assignment II on Spectroscopy 2024 Answers.pdfHome assignment II on Spectroscopy 2024 Answers.pdf
Home assignment II on Spectroscopy 2024 Answers.pdf
Tamralipta Mahavidyalaya
 
Language Across the Curriculm LAC B.Ed.
Language Across the  Curriculm LAC B.Ed.Language Across the  Curriculm LAC B.Ed.
Language Across the Curriculm LAC B.Ed.
Atul Kumar Singh
 
Guidance_and_Counselling.pdf B.Ed. 4th Semester
Guidance_and_Counselling.pdf B.Ed. 4th SemesterGuidance_and_Counselling.pdf B.Ed. 4th Semester
Guidance_and_Counselling.pdf B.Ed. 4th Semester
Atul Kumar Singh
 
Supporting (UKRI) OA monographs at Salford.pptx
Supporting (UKRI) OA monographs at Salford.pptxSupporting (UKRI) OA monographs at Salford.pptx
Supporting (UKRI) OA monographs at Salford.pptx
Jisc
 
CLASS 11 CBSE B.St Project AIDS TO TRADE - INSURANCE
CLASS 11 CBSE B.St Project AIDS TO TRADE - INSURANCECLASS 11 CBSE B.St Project AIDS TO TRADE - INSURANCE
CLASS 11 CBSE B.St Project AIDS TO TRADE - INSURANCE
BhavyaRajput3
 
The basics of sentences session 5pptx.pptx
The basics of sentences session 5pptx.pptxThe basics of sentences session 5pptx.pptx
The basics of sentences session 5pptx.pptx
heathfieldcps1
 
Model Attribute Check Company Auto Property
Model Attribute  Check Company Auto PropertyModel Attribute  Check Company Auto Property
Model Attribute Check Company Auto Property
Celine George
 
The Roman Empire A Historical Colossus.pdf
The Roman Empire A Historical Colossus.pdfThe Roman Empire A Historical Colossus.pdf
The Roman Empire A Historical Colossus.pdf
kaushalkr1407
 
Phrasal Verbs.XXXXXXXXXXXXXXXXXXXXXXXXXX
Phrasal Verbs.XXXXXXXXXXXXXXXXXXXXXXXXXXPhrasal Verbs.XXXXXXXXXXXXXXXXXXXXXXXXXX
Phrasal Verbs.XXXXXXXXXXXXXXXXXXXXXXXXXX
MIRIAMSALINAS13
 
TESDA TM1 REVIEWER FOR NATIONAL ASSESSMENT WRITTEN AND ORAL QUESTIONS WITH A...
TESDA TM1 REVIEWER  FOR NATIONAL ASSESSMENT WRITTEN AND ORAL QUESTIONS WITH A...TESDA TM1 REVIEWER  FOR NATIONAL ASSESSMENT WRITTEN AND ORAL QUESTIONS WITH A...
TESDA TM1 REVIEWER FOR NATIONAL ASSESSMENT WRITTEN AND ORAL QUESTIONS WITH A...
EugeneSaldivar
 

Recently uploaded (20)

The geography of Taylor Swift - some ideas
The geography of Taylor Swift - some ideasThe geography of Taylor Swift - some ideas
The geography of Taylor Swift - some ideas
 
2024.06.01 Introducing a competency framework for languag learning materials ...
2024.06.01 Introducing a competency framework for languag learning materials ...2024.06.01 Introducing a competency framework for languag learning materials ...
2024.06.01 Introducing a competency framework for languag learning materials ...
 
The Challenger.pdf DNHS Official Publication
The Challenger.pdf DNHS Official PublicationThe Challenger.pdf DNHS Official Publication
The Challenger.pdf DNHS Official Publication
 
The approach at University of Liverpool.pptx
The approach at University of Liverpool.pptxThe approach at University of Liverpool.pptx
The approach at University of Liverpool.pptx
 
Chapter 3 - Islamic Banking Products and Services.pptx
Chapter 3 - Islamic Banking Products and Services.pptxChapter 3 - Islamic Banking Products and Services.pptx
Chapter 3 - Islamic Banking Products and Services.pptx
 
Synthetic Fiber Construction in lab .pptx
Synthetic Fiber Construction in lab .pptxSynthetic Fiber Construction in lab .pptx
Synthetic Fiber Construction in lab .pptx
 
Mule 4.6 & Java 17 Upgrade | MuleSoft Mysore Meetup #46
Mule 4.6 & Java 17 Upgrade | MuleSoft Mysore Meetup #46Mule 4.6 & Java 17 Upgrade | MuleSoft Mysore Meetup #46
Mule 4.6 & Java 17 Upgrade | MuleSoft Mysore Meetup #46
 
Polish students' mobility in the Czech Republic
Polish students' mobility in the Czech RepublicPolish students' mobility in the Czech Republic
Polish students' mobility in the Czech Republic
 
Francesca Gottschalk - How can education support child empowerment.pptx
Francesca Gottschalk - How can education support child empowerment.pptxFrancesca Gottschalk - How can education support child empowerment.pptx
Francesca Gottschalk - How can education support child empowerment.pptx
 
Palestine last event orientationfvgnh .pptx
Palestine last event orientationfvgnh .pptxPalestine last event orientationfvgnh .pptx
Palestine last event orientationfvgnh .pptx
 
Home assignment II on Spectroscopy 2024 Answers.pdf
Home assignment II on Spectroscopy 2024 Answers.pdfHome assignment II on Spectroscopy 2024 Answers.pdf
Home assignment II on Spectroscopy 2024 Answers.pdf
 
Language Across the Curriculm LAC B.Ed.
Language Across the  Curriculm LAC B.Ed.Language Across the  Curriculm LAC B.Ed.
Language Across the Curriculm LAC B.Ed.
 
Guidance_and_Counselling.pdf B.Ed. 4th Semester
Guidance_and_Counselling.pdf B.Ed. 4th SemesterGuidance_and_Counselling.pdf B.Ed. 4th Semester
Guidance_and_Counselling.pdf B.Ed. 4th Semester
 
Supporting (UKRI) OA monographs at Salford.pptx
Supporting (UKRI) OA monographs at Salford.pptxSupporting (UKRI) OA monographs at Salford.pptx
Supporting (UKRI) OA monographs at Salford.pptx
 
CLASS 11 CBSE B.St Project AIDS TO TRADE - INSURANCE
CLASS 11 CBSE B.St Project AIDS TO TRADE - INSURANCECLASS 11 CBSE B.St Project AIDS TO TRADE - INSURANCE
CLASS 11 CBSE B.St Project AIDS TO TRADE - INSURANCE
 
The basics of sentences session 5pptx.pptx
The basics of sentences session 5pptx.pptxThe basics of sentences session 5pptx.pptx
The basics of sentences session 5pptx.pptx
 
Model Attribute Check Company Auto Property
Model Attribute  Check Company Auto PropertyModel Attribute  Check Company Auto Property
Model Attribute Check Company Auto Property
 
The Roman Empire A Historical Colossus.pdf
The Roman Empire A Historical Colossus.pdfThe Roman Empire A Historical Colossus.pdf
The Roman Empire A Historical Colossus.pdf
 
Phrasal Verbs.XXXXXXXXXXXXXXXXXXXXXXXXXX
Phrasal Verbs.XXXXXXXXXXXXXXXXXXXXXXXXXXPhrasal Verbs.XXXXXXXXXXXXXXXXXXXXXXXXXX
Phrasal Verbs.XXXXXXXXXXXXXXXXXXXXXXXXXX
 
TESDA TM1 REVIEWER FOR NATIONAL ASSESSMENT WRITTEN AND ORAL QUESTIONS WITH A...
TESDA TM1 REVIEWER  FOR NATIONAL ASSESSMENT WRITTEN AND ORAL QUESTIONS WITH A...TESDA TM1 REVIEWER  FOR NATIONAL ASSESSMENT WRITTEN AND ORAL QUESTIONS WITH A...
TESDA TM1 REVIEWER FOR NATIONAL ASSESSMENT WRITTEN AND ORAL QUESTIONS WITH A...
 

MS Thesis Defense Presentation

  • 1. Ghulam Destgeer Particle Separation and Chemical Gradient Control via Focused Travelling Surface Acoustic Waves (F-TSAW) Flow Control Laboratory, Department of Mechanical Engineering 2013.06.10
  • 2. 2 Contents • Introduction • Theory • Device design and fabrication • Experimental setup • Results • Summary
  • 4. 4 Particle separation • The isolation and separation of micro particulate materials in a continuous flow are required for chemical syntheses and biological analyses. • The separation and sorting of cells are critical in a variety of biomedical applications including: i. Diagnostics ii. Therapeutics iii. Cell biology <Lee et al., 2010, Lab Chip> <Daniel et al., 2010, Anal Bioanal Chem> Huang’s group Sung’s group
  • 5. 5 Particle manipulation by SSAW • Particle separation: – Particle diameter: 0.87μm (Red), 4.16μm (Green) • Experimental parameters: – Frequency: 12.6MHz – Power: 15-22dBm (30-160mW) – Flow rate: 0.6-2μl/min <Shi et al., 2009, Lab Chip>
  • 6. 6 Chemical gradient control • Most methods are capable of generating linear chemical gradient profiles in a static manner. • Generating pulsatile chemical gradients in microfluidic devices has important implications for the characterization of dynamic biological and chemical processes. • Dynamic temporal control of chemical gradients is required. <Ahmed et al., 2013, Lab Chip> <Daniel et al., 2006, Anal. Chem.> <Seidi et al., 2011, Biomicrofluidics>
  • 7. 7 Chemical gradient control by oscillating bubbles • Chemical solutions: – Dextran-FITC (stimulant) – Phosphate buffered saline(buffer) • Input voltage and frequency: – 12-16Vpp and 30kHz <Ahmed et al., 2013, Lab Chip>
  • 8. 8 Objective • (a) Device schematic (b) Particle separation • (c) Chemical gradient control and uniform micromixing • (d) F-TSAW amplitude (e) Fabricated device
  • 10. 10 Acoustic radiation force on compressible spheres For TSAW: <Yosioka & Kawasima, 1955, Acoustica> 𝐸 𝑎𝑐 = (4π𝑓𝑢)2 𝜌 𝑓 𝐴 2 = 2(4π𝑓𝑢)2 𝑘2 = 8(𝑐𝑢)2𝐴 2 = 2 𝐸 𝑎𝑐 𝑘2 𝜌 𝑓 where, 𝐸 𝑎𝑐 is acoustic energy density, 𝑢 is SAW amplitude, 𝑘 = 2π λ is wavenumber, 𝑐 is speed of sound on wafer surface, 𝑓 is the frequency of SAW, Pin is the input power and V is the input voltage. where, 𝛼 = 𝜌 𝑓 𝜌 𝑝 , 𝛽 = 𝑐 𝑓 𝑐 𝑝 , R is radius of μ-particles & A is complex amplitude of the velocity potential. 𝐹 𝑇𝑆𝐴𝑊 = 2𝜋𝜌 𝑓 𝐴 2(𝑘𝑅)6φ 𝑇𝑆𝐴𝑊 φ 𝑇𝑆𝐴𝑊 = 1 − 𝛼 2 + 𝛼 𝛽2 3 2 + 2 1 − 𝛼 2 9 (2 + 𝛼)2 TSAW Flow 𝐹 𝑇𝑆𝐴𝑊 ~ 𝑉2 𝑓6 𝑅6 ~ Pin 𝑓6 𝑅6
  • 11. 11 F-TSAW amplitude • Acoustic wave amplitude is estimated as: • Acoustic wave amplitude qualitative measure: 𝑢(𝑥, 𝑧) ≈ 1 𝑍1/4 −∞ +∞ 𝐺 𝑡 exp 𝑗 𝑡4 +𝑍′ 𝑡2 +𝑋′ 𝑡 𝑑𝑡 𝑍′ = (0.145𝑍 − 𝑅𝑘0/2) 4.98𝑍 𝑋′ = −𝑋 4 4.98𝑍 𝑡 = 4 4.98𝑍𝐾1 𝑍 = 𝑘0 𝑧 𝑋 = 𝑘0 𝑥 𝐾1 = 𝑘1/𝑘0 <Fang and Zhang, 1989, IEEE Transactions on Ultrasonic> <Wu et al., 2005, IEEE Transactions on Ultrasonic> Wu et al. 2005 Calculated θ R A A’
  • 12. 12 F-TSAW amplitude 5R 5R 5R 5R 5R 5R 5R 5R 5R AmplitudeAmplitudeAmplitude AmplitudeAmplitudeAmplitude AmplitudeAmplitudeAmplitude
  • 14. 14 SAW amplitude calculation F 𝑇𝑆𝐴𝑊~ (Eac/k2) (kR)6φ 𝑇𝑆𝐴𝑊 E 𝑎𝑐~ u2 f2 ρ Energy density (Eac) – J/m3 SAW displacement (u) – nm Frequency (f) – MHz Density (ρ) – kg/m3 Wave number (k) – (μm)-1 Particle radium (R) – (μm) Constant (φ) Contour plots of SAW displacement square (u2) – m2 Top – f =133.3MHz Bottom – f = 40.0MHz x z
  • 15. Device design and fabrication
  • 16. 16 F-TSAW device design • Two salient features: (i) unidirectional (ii) focused • Interdigitated transducer(IDT): Two interlocking comb-shaped metallic electrodes on top of a piezoelectric substrate. • Frequency of applied AC signal = frequency of SAW (fSAW) – fSAW = c/λ, c is speed of sound in the piezoelectric substrate Maximum energy is transmitted in the forward direction. Very little energy is transmitted in the backward direction. SAW λ λ/8 λ/43λ/16 SAW Unidirectional transducer λλ/4 SAW SAW IDT F-TSAW amplitude by a focusing transducer
  • 21. 21 Experiment schematic Signal generator, N5181A [3GHz] μ-pump, neMESYS Microscope, BX51 Camera, DP26 Power amplifier, ZHL-1-2W DC power supply, E3634A Micro Chip PDMSLiNbO3 Au electrodes
  • 22. 22 Experimental setup Power supply Amplifier Signal generator Microscope Microchip Micropump Display screen Oscilloscope Camera
  • 24. 24 Experimental parameters PARAMETERS Device #1 Device #2 Device #3 Device #4 Frequency (MHz) 40 133.3 133.3 133.3 Input power 0.25µW 0.45mW 0.07mW --- After amplification 0.175mW 275mW 63mW 60–200mW Radius of FUT (mm) 6 4 4 4 Distance from FUT to microchannel 1.25R 2.5R 2.5R 2.5R µ-channel cross section (µm×µm) 150×110 150×45 200×40 500×90 Particles diameter (µm) 30 and 10 10 and 3 10 and 3 --- Fluid/media DI water DI water DI water DI water, rhodamine Total flow rate (µl/hr) 50 150 100 100 Average velocity (mm/s) 0.84 6.17 3.5 0.6 Function Particle Separation Particle Separation Particle Separation Gradient Generation Names CAPS-1 CAPS-2 CAPS-3 CAGG *CAPS: Cross-type Acoustic Particle Separator *CAGG: Cross-type Acoustic Gradient Generator
  • 25. 25 CAPS-1: Particle trajectory and separation • Experimental conditions: – Frequency (f): 40MHz (Low) – Input power: 725µW – Flow rate (Q): 50μl/h (0.84mm/s) – μ-channel cross-section: 150x110μm – μ-particles diameter: 10, 30μm • Equation of particle motion: • Acoustic radiation force: • Stokes drag force: • Particle trajectory: – Left figure: Theoretical – Center figure: Experimental • Particle separation on right 𝑚 𝑧 = 𝐹 𝑇𝑆𝐴𝑊 − 𝐹 𝐷 𝐹 𝑇𝑆𝐴𝑊 = 4π𝐸 𝑎𝑐 𝑘4 𝑅6φ 𝑇𝑆𝐴𝑊 𝐹 𝐷 = 6πη𝑅 𝑧
  • 26. 26 CAPS-2: Particle trajectory and separation • Experimental conditions: – Frequency (f): 133.3MHz (High) – Input power: 1.36W – Flow rate (Q): 150μl/h (6.17mm/s) – μ-channel cross-section: 150x45μm – μ-particles diameter: 10, 3μm • (a) Schematic diagram of a PDMS microchannel. • (b-c) Once the TSAW was turned ON, a distinct separation distance could be observed. • (d) Trajectory followed by a 10 µm particle influenced by acoustic streaming.
  • 27. 27 CAPS-3: Particle trajectory and separation • Experimental conditions: – Frequency: 133.3MHz – Input power: 225mW – μ-channel cross-section: • h x w: 40 x 200 μm – Flow rate (Q): • Sample+ Sheath: 25μl/h + 75μl/h = 100μl/h • Average speed: 3.5mm/s – μ-particles diameter: 3μm and 10μm • Left: TSAW OFF, all the particles flowing together with the laminar flow. • Right: TSAW ON, larger particles are pushed towards the opposite wall resulting in separation
  • 28. 28 Particle separation efficiency • (a) TSAW OFF: all of the particles are collected at the same outlet • (b) TSAW ON: 3µm particles are collected at same outlet whereas almost 100% of the 10µm particles passed through a separate outlet. (a) (b)
  • 29. 29 CAPS-3: Particle deflection vs. input power • Flow rate is kept constant: – Sheath + Sample = 80 + 20 = 100 µlh-1
  • 30. 30 CAPS-3: Deflection vs. input power and flow rate • For particles with diameter 10µm:
  • 31. 31 CAPS-3: Deflection (µm) vs. Input Power (mW)
  • 32. 32 CAGG • Acoustic streaming flow induced via F-TSAW • Flow is traced by 1µm polymer microspheres dispersed in DI water. • On smaller particles, drag force is dominant compared to acoustic radiation force. • Three microchannels 150µm x 45µm, 200µm x 40µm and 500µm x 90µm from left to right, respectively, are tested. • Microchannel 500µm x 90µm can produce strong and large vortices appropriate for mixing and gradient control. F-TSAW F-TSAW
  • 33. 33 Chemical gradient control and micromixing • Acoustic streaming flow – Generate chemical gradient – Uniformly mix fluids. • Microchannel – w×h: 500µm×90µm • Flow rate: 100µl/h (0.6mm/s) – Fluid 1: rhodamine: 50µl/h – Fluid 2: DI water: 50 µl/h • Power input – Gradient control: 60–200mW (18– 23dBm) – Uniform mixing: 800mW (29dBm)
  • 34. 34 Chemical gradient control and micromixing
  • 35. 35 Summary • Four types of devices are tested: – First three are Cross-type Acoustic Particle Separator (CAPS) – Fourth is Cross-type Acoustic Gradient Generator (CAGG) • A single micro-chip is capable to be used as CAPS or CAGG • Particles are successfully separated with efficiency close to 100%: – 10μm particles from 3μm and 30μm particles from 10μm • Particle deflection is plotted against input power which shows: – 3μm, 7μm and 10μm are separated • Low amplitude and high frequency (40 and 133.3MHz) waves are used. • Chemical gradient control and uniform mixing is also shown using F-TSAW without trapping any micro-bubble.
  • 36. THANK YOU FOR YOUR ATTENTION!!!