The guest lecture, given by Dr. Alaa Khamis at the University of Waterloo for ECE 486, focuses on the fundamentals of motion planning in robotics, covering concepts such as path planning, planning algorithms, and trajectory planning for robot manipulators. Key topics include discrete and combinatorial planning methods, configuration space representation, and the requirements of effective path planners. The lecture emphasizes the practical application of these concepts across multiple robotic systems and discusses the challenges of determining collision-free paths in dynamic environments.