Genetic variation exists among potato cultivars for nitrogen use efficiency (NUE) and related traits under different nitrogen levels. The study evaluated 97 potato cultivars across two locations in Ethiopia under low and high nitrogen conditions. Results showed significant effects of location, nitrogen level, and genotype on canopy development, tuber yield, and NUE. Heritability estimates for tuber number, NUE, and other traits were high, indicating potential for genetic improvement. Traits like tuber number, canopy area, days to maturity, and tuber dry matter content were strongly correlated with NUE and thus important for selecting cultivars with higher NUE. The study found genetic diversity in the potato germplasm that can