With decades of experience in the fields of genomics sequencing, CD Genomics is devoted to providing unprecedented amounts of microbial metatranscriptomic data. Our strong expertise in the informative and unbiased metatranscriptomic sequencing service is guaranteed by state-of-the-art high throughput sequencers, flexible sequencing strategies, and professional bioinformatics pipelines.
Metagenomics is the study of genetic material recovered directly from environmental samples. Metagenomics is a molecular tool used to analyse DNA acquired from environmental samples, in order to study the community of microorganisms present, without the necessity of obtaining pure cultures.
Metagenomics is the study of metagenome, genetics material, recovered directly from environmental sample such as soil, water or faeces.
Metagenomics is based on the genomics analysis of microbial DNA directly
from the communities present in samples
Metagenomics technology – genomics on a large scale will probably lead to great advances in medicine, agriculture, energy production and bioremediation.
Metagenomics can unlock the massive uncultured microbial diversity present in the environment for new molecule for therapeutic and biotechnological application.
Metagenomic studies have identified many novel microbial genes coding for metabolic pathways such as energy acquisition, carbon and nitrogen metabolism in natural environments that were previously considered to lack such metabolism
Microbiology has experienced a transformation during the last 25 years that has altered microbiologists' view of microorganisms and how to study them. The realization that most microorganisms cannot be grown readily in pure culture forced microbiologists to question their belief that the microbial world had been conquered. We were forced to replace this belief with an acknowledgment of the extent of our ignorance about the range of metabolic and organismal diversity.
Clinical Metagenomics for Rapid Detection of Enteric Pathogens and Characteri...QIAGEN
High-throughput sequencing, combined with high-resolution metagenomic analysis, provides a powerful diagnostic tool for clinical management of enteric disease. Forty-five patient samples of known and unknown disease etiology and 20 samples from health individuals were subjected to next-generation sequencing. Subsequent metagenomic analysis identified all microorganisms (bacteria, viruses, fungi and parasites) in the samples, including the expected pathogens in the samples of known etiology. Multiple pathogens were detected in the individual samples, providing evidence for polymicrobial infection. Patients were clearly differentiated from healthy individuals based on microorganism abundance and diversity. The speed, accuracy and actionable features of CosmosID bioinformatics and curated GenBook® databases, implemented in the QIAGEN Microbial Genomics Pro Suite, and the functional analysis, leveraging the QIAGEN functional metagenomics workflow, provide a powerful tool contributing to the revolution in clinical diagnostics, prophylactics and therapeutics that is now in progress globally.
Metagenomics is the study of genetic material recovered directly from environmental samples. Metagenomics is a molecular tool used to analyse DNA acquired from environmental samples, in order to study the community of microorganisms present, without the necessity of obtaining pure cultures.
Metagenomics is the study of metagenome, genetics material, recovered directly from environmental sample such as soil, water or faeces.
Metagenomics is based on the genomics analysis of microbial DNA directly
from the communities present in samples
Metagenomics technology – genomics on a large scale will probably lead to great advances in medicine, agriculture, energy production and bioremediation.
Metagenomics can unlock the massive uncultured microbial diversity present in the environment for new molecule for therapeutic and biotechnological application.
Metagenomic studies have identified many novel microbial genes coding for metabolic pathways such as energy acquisition, carbon and nitrogen metabolism in natural environments that were previously considered to lack such metabolism
Microbiology has experienced a transformation during the last 25 years that has altered microbiologists' view of microorganisms and how to study them. The realization that most microorganisms cannot be grown readily in pure culture forced microbiologists to question their belief that the microbial world had been conquered. We were forced to replace this belief with an acknowledgment of the extent of our ignorance about the range of metabolic and organismal diversity.
Clinical Metagenomics for Rapid Detection of Enteric Pathogens and Characteri...QIAGEN
High-throughput sequencing, combined with high-resolution metagenomic analysis, provides a powerful diagnostic tool for clinical management of enteric disease. Forty-five patient samples of known and unknown disease etiology and 20 samples from health individuals were subjected to next-generation sequencing. Subsequent metagenomic analysis identified all microorganisms (bacteria, viruses, fungi and parasites) in the samples, including the expected pathogens in the samples of known etiology. Multiple pathogens were detected in the individual samples, providing evidence for polymicrobial infection. Patients were clearly differentiated from healthy individuals based on microorganism abundance and diversity. The speed, accuracy and actionable features of CosmosID bioinformatics and curated GenBook® databases, implemented in the QIAGEN Microbial Genomics Pro Suite, and the functional analysis, leveraging the QIAGEN functional metagenomics workflow, provide a powerful tool contributing to the revolution in clinical diagnostics, prophylactics and therapeutics that is now in progress globally.
This presentation elaborates on the process through which bacteria communicate with each other using signalling molecules which they can produce and receive.
Preparatiion of competent cells & Transformation Practical Sabahat Ali
Competence is ability of bacteria to take up foreign DNA.
Transformation is alteration of genetic material resulting from uptake of exogenous genetic material from surrounding environment through cell membrane .
Metagenomics is the study of a collection of genetic material (genomes) from a mixed community of organisms. Metagenomics usually refers to the study of microbial communities.
Metatranscriptomics is the science that studies gene expression of microbes within natural environments, i.e., the metatranscriptome. It also allows to obtain whole gene expression profiling of complex microbial communities.
This presentation was given to me during my higher education in the Lebanese University, Faculty of Education. It includes detailed explanation about DNA microarray.
Probes are used for hybridization purposes. different types of probes can be used on the basis of what we want to hybridize. May be Radioactive or Non-Radioactive.
Ribotyping
Introduction
History
Ribosomes
Ribosomal RNA
Principle of ribotyping
16S rRNA
Procedure of ribotyping
Types of ribotyping
Use of ribotyping
Advantage and disadvantage of ribotyping
Reference
nitrate and sulfate reduction ; methanogenesis and acetogenesisjyoti arora
this powerpoint includes the following topics in brief:
1. Nitrate assimilatory reduction
2. Sulfate assimilatory reduction
3. Methanogenesis
4. Acetogenesis
Traditional culture-dependent microorganism identification methods lean heavily on morphology, physiology, pathology, and biochemical testing, which are time consuming and labor-intensive. The advances of genetic technologies in recent decades have shined a light on microbial identification and promoted the soaring of microbiota research and the progress of medical science. https://www.cd-genomics.com/microbioseq/microseq-microbial-identification.html
Traditional culture-dependent microorganism identification methods lean heavily on morphology, physiology, pathology, and biochemical testing, which are time consuming and labor-intensive. The advances of genetic technologies in recent decades have shined a light on microbial identification and promoted the soaring of microbiota research and the progress of medical science. https://www.cd-genomics.com/microbioseq/microseq-microbial-identification.html
This presentation elaborates on the process through which bacteria communicate with each other using signalling molecules which they can produce and receive.
Preparatiion of competent cells & Transformation Practical Sabahat Ali
Competence is ability of bacteria to take up foreign DNA.
Transformation is alteration of genetic material resulting from uptake of exogenous genetic material from surrounding environment through cell membrane .
Metagenomics is the study of a collection of genetic material (genomes) from a mixed community of organisms. Metagenomics usually refers to the study of microbial communities.
Metatranscriptomics is the science that studies gene expression of microbes within natural environments, i.e., the metatranscriptome. It also allows to obtain whole gene expression profiling of complex microbial communities.
This presentation was given to me during my higher education in the Lebanese University, Faculty of Education. It includes detailed explanation about DNA microarray.
Probes are used for hybridization purposes. different types of probes can be used on the basis of what we want to hybridize. May be Radioactive or Non-Radioactive.
Ribotyping
Introduction
History
Ribosomes
Ribosomal RNA
Principle of ribotyping
16S rRNA
Procedure of ribotyping
Types of ribotyping
Use of ribotyping
Advantage and disadvantage of ribotyping
Reference
nitrate and sulfate reduction ; methanogenesis and acetogenesisjyoti arora
this powerpoint includes the following topics in brief:
1. Nitrate assimilatory reduction
2. Sulfate assimilatory reduction
3. Methanogenesis
4. Acetogenesis
Traditional culture-dependent microorganism identification methods lean heavily on morphology, physiology, pathology, and biochemical testing, which are time consuming and labor-intensive. The advances of genetic technologies in recent decades have shined a light on microbial identification and promoted the soaring of microbiota research and the progress of medical science. https://www.cd-genomics.com/microbioseq/microseq-microbial-identification.html
Traditional culture-dependent microorganism identification methods lean heavily on morphology, physiology, pathology, and biochemical testing, which are time consuming and labor-intensive. The advances of genetic technologies in recent decades have shined a light on microbial identification and promoted the soaring of microbiota research and the progress of medical science. https://www.cd-genomics.com/microbioseq/microseq-microbial-identification.html
Extracellular Biomarkers Summit, the newest addition to Cambridge Healthtech Institute's notable biomarker and diagnostics portfolio, merges the well-established microRNA as Biomarkers and Diagnostics Conference with focused discussions on three leading areas of research: the role of circulating microRNA, exosomes and microvesicles, long non-coding RNA and extracellular RNA in cancer and other diseases, as well as their potential to serve as biomarkers in drug and diagnostic development. Discussions will include the isolation and characterization of exosomes and exRNA, including expression profiling and sequencing of exRNA; understanding the role of miRNA, lncRNA, and exosomes in disease mechanism, tumor metastasis, and intracellular communication; their potential as biomarkers in drug development, drug toxicity assessment, and patient stratification; and finally, their role as circulating biomarkers for disease diagnosis and prognosis. Learn more at http://www.extracellularbiomarkers.com
CD Genomics, experts in dna & genome sequencing service, genotyping, health diagnostics, bioinformatics, custom cdna library development. Contact Us Today!
Applying Innovative RNA-Seq Technology to Support Research & Drug Discovery
Informatics & Statistical Analysis of RNA-Seq Data
Transcriptomics: Microarray vs RNA-Seq
Discover new cases studies giving you unprecedented access to both the data and results of how RNA-Seq is being applied successfully from bench to bedside
Gain new insights into RNA-Seq for the study of toxicity, IO, host-viral interactions and more from companies such as BMS, Janssen, Pfizer, Merck, UCSC and Stanford
Course: Bioinformatics for Biomedical Research (2014).
Session: 3.2- Basic Aspects of Microarray Technology and Data Analysis.
Statistics and Bioinformatisc Unit (UEB) & High Technology Unit (UAT) from Vall d'Hebron Research Institute (www.vhir.org), Barcelona.
A microarray is a laboratory tool used to detect the expression of thousands of genes at the same time. DNA microarrays are microscope slides that are printed with thousands of tiny spots in defined positions, with each spot containing a known DNA sequence or gene.
We recently developed MVRSION for the purpose of using 16S to define microbiome bacterial populations from human, animal, or environmental sources. It is is a methodology that statistically leverages 8, rather than one, 16S hyper-variable regions.
Molecular Biology research evolves through the development of the technologies used for carrying them out. It is not possible to research on a large number of genes using traditional methods
Presentation by Justin Zook at GRC/GIAB ASHG 2017 workshop "Getting the most from the reference assembly and reference materials" on benchmarks for indels and structural variants.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
1. CONTACT
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-619-7922
Email: contact@cd-genomics.com
Web: https://www.cd-genomics.com
Metatranscriptomic Sequencing Service
By CD Genomics
With decades of experience in the fields of genomics sequencing, CD
Genomics is devoted to providing unprecedented amounts of microbial
metatranscriptomic data. Our strong expertise in the informative and unbiased
metatranscriptomic sequencing service is guaranteed by state-of-the-art high
throughput sequencers, flexible sequencing strategies, and professional
bioinformatics pipelines.
The Introduction of Metatranscriptomic Sequencing
Metatranscriptomics is the culture-independent profiling (including protein-
coding and non-coding DNA) of microbial community-wide gene expression,
which is capable of monitoring RNA-based regulation and expressed
biological signatures of complex bacterial communities in a given sample at a
given moment and under specific conditions. It elucidates three aspects of a
microbial community, including gene activity diversity, gene expression
abundance, and differential gene expression analysis. The gene expression
analysis can tell which genes exhibit the highest change in expression levels
in different conditions potentially to identify biomarkers and expression
signatures.
While metagenomics shows us the microbial species present in the
community and their genomic potentials, metatranscriptomics presents the
function and activity of the complete set of transcripts, as well as community
structure. Metatranscriptomics offers a more informative perspective
compared with metagenomics in revealing active biochemical functions, which
has become a focus for applications in the environmental, medical, and
energy fields as well as the field of drug discovery.
Advantages of Metatranscriptomic Sequencing
• A culture-free method to reveal the true extent of microbial diversity
• Permitting function-based activity screens
• More targeted than shotgun random sequencing
• Cost-efficient and time-effective
2. CONTACT
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-619-7922
Email: contact@cd-genomics.com
Web: https://www.cd-genomics.com
• A wide range of applications, including basic research, ecological applications, clinical
applications, industrial applications, and so on
Metatranscriptomic Sequencing Workflow
Our highly experienced expert team executes quality management following
every procedure to ensure confident and unbiased results. The general
workflow for metatranscriptomic sequencing is outlined below. After RNA
isolation from qualified samples, rRNA depletion, library construction, high-
throughput sequencing, and bioinformatics analysis are in turn performed. We
can help you make decisions on the library construction method, the depth of
coverage, and the data analysis strategies based on your research aims.
Service Specification
3. CONTACT
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-619-7922
Email: contact@cd-genomics.com
Web: https://www.cd-genomics.com
CD Genomics provides full metatranscriptomic sequencing service package
including sample standardization, library construction, Hiseq sequencing, raw
data alignment, down-stream bioinformatics processing and statistical
analysis. We can tailor this pipeline to your research interest. If you have
additional requirements or questions, please feel free to contact us, our
specialists are more than happy to assist you.
Reference:
1. Warnecke F, Hess M. A perspective: metatranscriptomics as a tool for the
discovery of novel biocatalysts. Journal of Biotechnology, 2009, 142(1): 91-95.
