The document discusses the management and use of laminar air flow for explant inoculation in tissue culture. It describes laminar air flow as an enclosed bench designed to provide sterile conditions through HEPA filtration and UV sterilization of air. Proper management includes sterilizing surfaces with ethanol before and after use, turning on UV lights 30 minutes prior, and flame sterilizing instruments. Explants are surface sterilized with mercuric chloride before trimming and inoculating into sterile culture media in the laminar flow hood. Inoculated cultures are then transferred to a sterilized culture room. Safety precautions for laminar flow use include switching off UV lights during use, cleaning hands with ethanol, and properly adjusting
Basic laboratory instruments
Principle and working of Laminar Air Flow
In this ppt all infermation given
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Introduction :
Antibiotics are antimicrobial agents produced naturally by other microbes (usually fungi or bacteria)
The first antibiotic was discovered in 1896 by Ernest Duchesne and in 1928 "rediscovered" by Alexander Fleming from the filamentous fungus Penicilium notatum.
The antibiotic substance, named penicillin, was not purified until the 1940s (by Florey and Chain), just in time to be used at the end of the second world war.
Penicillin was the first important commercial product produced by an aerobic, submerged fermentation
Basic laboratory instruments
Principle and working of Laminar Air Flow
In this ppt all infermation given
contact :
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Introduction :
Antibiotics are antimicrobial agents produced naturally by other microbes (usually fungi or bacteria)
The first antibiotic was discovered in 1896 by Ernest Duchesne and in 1928 "rediscovered" by Alexander Fleming from the filamentous fungus Penicilium notatum.
The antibiotic substance, named penicillin, was not purified until the 1940s (by Florey and Chain), just in time to be used at the end of the second world war.
Penicillin was the first important commercial product produced by an aerobic, submerged fermentation
This PPT will provide the basic idea of Fermentation technology and it's use. The reference book is 'Pharmaceutical Biotechnology' by Giriraj Kulkarni.
Preservation of industrially important microorganisms, methods of preservation, periodic transfer, storage in saline suspension, storage in sterile soil, cryopreservation
Callus cultures are initiated from a small part of an organ or tissue segment called the explants on a growth supporting solidified nutrient medium under sterile conditions. Any part of the plant organ or tissues may be used as the explants.Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. Plant tissue culture is widely used to produce clones of a plant in a method known as micropropagation.Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants.Plant Tissue Culture products include Murashige and Skoog media, plant growth regulators, plant growth hormones, plant transformation systems,
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This PPT will provide the basic idea of Fermentation technology and it's use. The reference book is 'Pharmaceutical Biotechnology' by Giriraj Kulkarni.
Preservation of industrially important microorganisms, methods of preservation, periodic transfer, storage in saline suspension, storage in sterile soil, cryopreservation
Callus cultures are initiated from a small part of an organ or tissue segment called the explants on a growth supporting solidified nutrient medium under sterile conditions. Any part of the plant organ or tissues may be used as the explants.Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. Plant tissue culture is widely used to produce clones of a plant in a method known as micropropagation.Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants.Plant Tissue Culture products include Murashige and Skoog media, plant growth regulators, plant growth hormones, plant transformation systems,
Laminar airflow manfacturer in chennai|india|bangalore|hyderabad|pune|delhi|m...velswamy
The Top Roofing contractors in Chennai are Sole Proprietorship (Individual) based firm, engaged as the trader and wholesaler of Roofing Contractors, Roofing Shed, Terrace Roofing and many more. Our Unique Selling Point is providing the best quality and Durable Goods in Reasonable Price.
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This is the 1st part of our "All about cleanrooms". This presentation will take you through the history of cleanrooms, types and applications of cleanrooms.
Laminar Air Flow provides a work area with Aseptic/Sterile conditions for th...AyushiSharma843565
Laminar Air Flow is an enclosed bench designed to prevent contaminations like biological particles or any particle sensitive device.
This closed cabinet is usually made up of stainless steel without any gap or joints where spores might collect.
Laminar Hoods are equipped with a shortwave ultraviolet germicidal lamp to sterilize the shell
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Maintenance of aseptic condition, in plant tissue cultureKAUSHAL SAHU
Introduction
Aseptic technique
Sterilizing the culture vessels and instruments
Sterilization of culture media
Sterilizing Transfer area
Sterilizing culture rooms
Sterilizing Plant material
Transfer of the explants
Conclusions
References
Designing of aseptic area, laminar flow equipment: Study of different source ...Ms. Pooja Bhandare
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Flow diagram of aseptic area. Floors, walls and ceilings, Doors, windows and services Personnel and protective clothing Cleaning and disinfection. Air Supply. Laminar flow equipment. Vertical laminar air flow bench
Horizontal laminar air flow bench
High Efficiency Particulate Air (HEPA) Filter. Operating Instructions Uses of Laminar Air Flow.Advantages of Laminar Air Flow.Limitations of Laminar Air Flow. Air flow pattern Unidirectional airflow
Non-unidirectional airflow
Combined airflow
Different Sources of Contamination in an Aseptic Area
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2) Buildings and Facilities
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Methods of Prevention of Contamination Clean Area Classification
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1. Management of Laminar Air Flow and
Inoculation of Explant
Presentated by:
Tanmoy Mondal
Reg.no:H-2014-19-B
2. What is Laminar Air Flow
Laminar Air Flow is an enclosed bench
designed to prevent contaminations like
biological particles or any particle sensitive
device.
This closed cabinet is usually made up of
stainless steel without any gap or joints
where spores might collect.
Laminar Hoods are equipped with a
shortwave ultraviolet germicidal lamp to
sterilize the shell
Use:Laminar Air Flow provides a work
area with aseptic/sterile conditions for the
tissue culture
3. History of Laminar Air Flow
• Laminar Air Flow is a fairly new
innovation.
• The idea of Laminar Air Flow was
develop by few scientist around
early 2000s.
• Johan Coecke et al. gave the
basic definition of Laminar Air
Flow in 2005 Johan Coecke et al.
4. Structure of Laminar air flow
Caster
HEPA filter
Control panel
UV light
Working Table
Pre-Filter
5. Working Principal
• A laminar flow hood consists of a filter pad, a
fan and a HEPA (High Efficiency Particulates
Air) filter.
• In a laminar flow hood the air is passed
through a HEPA (High Efficiency Particulates
Air) filter which removes all airborne
contamination to maintain sterile conditions.
• The fan sucks the air through the filter pad
where dust is trapped.
• After that the prefiltered air has to pass the
HEPA filter where contaminating fungi,
bacteria, dust etc are removed.
• Now the sterile air flows into the working area
where you can do all your flasking work
without risk of contamination.
6. Management of Laminar air flow
UV lamp that should be turned on about 30 minutes
before being used to sterilize the shell or cabinet or the
surface of the Laminar Air Flow to avoid any kind of
contaminations.
Wipe down the surface with 95% ethanol before and
after each use.
After inoculation, the cultures were transferred to the
culture room which was initially sterilized by washing all
of its walls and floor
7. Management of Laminar air flow
The instruments like
foreceps, inoculating
neadles, the scalpel blade
etc.were flame sterilized
before using them for the
inoculation.
The finger tips were cleaned
by 70% ethyl alcohol
Mouth and nose were
covered with a mask so as to
avoid the contamination of
the cultures.
Flame Sterilization
8. Surface Sterilization of Explant
• The explants wear treated
with 0.1% Mercuric
Chloride (HgCl2)for 10
minutes
Explant
9. Preparation of Explant
• The explants were further
trimmed and extra outer
portain wear removed to
make them stable size.
Trimming explant
10. Inoculation of Explant
• After cutting of explant were
transfer to culture bottle
containing MS media
• Innoculated plant then
transfer to culture room
Inoculation
12. Safety & Precautions taken for Laminar Hood
It is important to switch off UV light during use as it may cause mutations
Large objects should never be placed near the back of the hood. Which
normally suspends the contaminants and removes them from the area.
Waste and other items should never enter the hood.
Hands should be cleaned by ethanol.
Do not touch hair, face or clothing while working.
Excess dust should be removed from items before introducing them into
the hood.
Always handle open bottle at an angle; never let them point directly up,
Properly adjust the flame of the Bunsen burner. The proper flame is a
small blue cone; it is not a large plume, nor is it orange.