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Light microscopy dr muthulakshmi
The document discusses microscopy, including the properties of light, components of the light microscope, and different types of microscopy. It describes the parts of the microscope like the eyepiece, objective lens, body tube, and stage. It explains concepts such as magnification, resolution, numerical aperture, reflection, refraction, and dispersion. The document also classifies microscopy techniques including phase contrast and fluorescence microscopy.
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Light microscopy dr muthulakshmi
- 1. DR. MUTHULAKSHMI C Postgraduate student Department of Oral Pathology & Microbiology SRM Dental College, Ramapuram, Chennai, India MICROSCOPY:INTRODUCTION TO MICROSCOPY, PROPERTIES OF LIGHT,COMPONENTS OF LIGHT MICROSCOPE(LENS SYSTEM,MAGNIFICATION VALUES,ADJUSTING THE LIGHT FIELD)
- 2. SYNOPSIS LIGHT AND INTRODUCTIONTO LIGHT PROPERTIES OF LIGHT INTRODUCTION OF MICROSCOPE PARTS OF MICROSCOPE FORMATION OF AN IMAGE MAGNIFICATION CLASSIFICATION OF MICROSCOPY DIFFERENT TYPES OF MICROSCOPY
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- 4. The part ofelectromagnetic spectrum between approximately 380 nm to 780 nm can be perceived by human visual system is called as VISIBLE LIGHT
- 5. PROPERTIES OF LIGHT: Interaction of light with matter. Absorption Transmission Scattering Reflection Refraction Dispersion Polarization Interference Diffraction
- 6. INTERACTION OF LIGHTWITH MATTER
- 7. ABSORPTION LIGHT isabsorbed by the medium through which it passes. This can reduce the amplitude or energy level of the light. The medium through which light passes can also have an effect on actual speed at which light passes through the material, referred as retardation
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- 22. PARTS OF MICROSCOPE: OPTICAL COMPONENTS •Eyepiece (oculars) • Objectives MECHANICAL COMPONENTS • Body Tube • Adjustment • Stage ILLUMINATION COMPONENTS • Sub stage • Condenser • Iris Diaphragm • Filter Carrier • Mirror • Light Source
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- 28. minimum amount oflight from the object, unite it , a high quality magnified real image. The ability of an objective to resolve the detail is indicated by its numerical aperture
- 29. The numerical aperture(NA) of the objective lens varies from 0.16 to 1.40 , depending on the type of lens. The numerical aperture (NA) is expressed as a value and will be found engraved on the body of the objective. NA=nSinµ ’n’ is the refractive index of the medium between the Cover glass over the object and the front lens of the objective ‘µ’ is the angle included between the optical axis of the lens and the outermost ray that can enter the front lens.
- 30. Resolution does notdepend entirely on the NA of a lens but also on the wavelength of the light used λ resolution /NA = 0.61λ Resolution is the smallest distance between two dots or lines λ is the wavelength of the light. The resolving powerof the objective is its ability to resolve the detail that can be measured NA ∝Resolution Power 1/ ∝working distance, flatness of field and focal length.
- 31. CLASSIFICATION OF THEOBJECTIVE LENS Apochromatic objectives Achromatic objectives semiachromat or Fluorite objectives Plan- apochromat objectives Polarising objectives Phase objectives
- 39. Different sources of light are Tungsten- Halogen Lamps Mercury Arc Lamps Xenon Arc Lamps Metal HalideArc Lamps Light- Emitting Diodes
- 44. MAGNIFICATION VALUE: Total magnificationis the product of the magnification values of the objective and eyepiece ,provided the system is standardized to an optical tube length of 160 mm. OPTICAL TUBE LENGTH X MAGNIFICATION OF FOCAL LENGTH OF OBJECTIVE EYEPIECE objective 40 x ,eyepiece 10 x, tube lens factor 1.25x gives a total magnification value of 500x
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