Antigen retrieval is a technique used to expose antigens in tissue samples that were previously masked during the tissue processing and fixation steps. There are two main antigen retrieval methods - proteolytic enzyme digestion and heat induced epitope retrieval. Proteolytic enzyme digestion uses enzymes like trypsin, pepsin, or protease to break down cross-links formed during fixation. Heat induced epitope retrieval uses heat, typically in a pressure cooker or microwave, to reverse masking by weakening calcium bonds or cleaving methylene bridges. The standard immunohistochemistry staining procedure after antigen retrieval involves blocking, primary antibody incubation, secondary antibody or streptavidin incubation, substrate application, and counterstaining.