techniques used for preparing serial sections using microtomes include dehydrating agents and clearing agents ,this slide includes some details on dehydrating and clearing agents
This document provides summaries of several common laboratory techniques and procedures:
1. It describes how to properly measure volume using graduated cylinders and pipettes, and how to read meniscus levels.
2. It explains how to weigh samples using balances, including zeroing the balance and using tare functions.
3. Common separation techniques like decantation, filtration, centrifugation, distillation, and chromatography are summarized.
This document describes various pieces of lab equipment including beakers, Erlenmeyer flasks, volumetric flasks, graduated cylinders, test tubes, tongs, test tube holders, test tube brushes, scoopulas, thermometers, forceps, medicine droppers, test tube racks, stirring rods, Bunsen burners, retort stands, C-clamps, burettes, wire mesh, clay triangles, crucibles, funnels, filter paper, mortar and pestles, spot plates, evaporating dishes, Petri dishes, and watch glasses. Each item is briefly described in terms of its common laboratory uses and characteristics.
The document provides guidance for safely conducting laboratory experiments. It lists tips for laboratory set-up and procedures, such as assembling apparatus away from edges, following experimental procedures exactly unless instructed otherwise, avoiding distractions, reading labels carefully, and informing instructors of any accidents or injuries. Personal protective equipment like closed shoes and secured hair are required. Chemicals should be treated as dangerous, and consumption or misuse is forbidden. Proper documentation and cleanup are also emphasized.
This document provides a rubric for evaluating 3D atomic model projects created by students using Bhor's atomic model. The rubric assesses students on accuracy of the atomic model, creativity, design/materials used, and labeling. For an accurate model, the number of protons, neutrons, and electrons must match the assigned element. Creativity is judged based on uniqueness of design. Design and materials are evaluated on appropriateness and quality of construction. Labels on the model and information card must include the element name, symbol, atomic number, and mass.
The water bath is an
instrument used in the
laboratory for carrying out
serological, biomedical,
and pharmaceutical tests at
specific temperature ranges.
as a partial requirement for one of my subject for this semester
I would like you to view my presentation and comment as well
I will be very glad if you find my presentation interesting, or comment on how I can improve my craft, THANK YOU :)
Analytical balances are highly precise instruments used to determine small mass measurements. They can measure up to four decimal places of grams and are sensitive enough to detect changes as small as 0.0001 g. Due to their precision, they must be used in draft-shielded environments and powered by electricity. Analytical balances are used to weigh small quantities in the milligram range when great accuracy is required, such as pharmaceutical applications. Proper use requires leveling the balance, zeroing it, and either directly placing solid samples or taring containers for liquids or powders before taking measurements.
This document describes various types of laboratory equipment used in medical laboratories. It discusses microscopes for examining small organisms, incubators and autoclaves for sterilizing culture media and equipment, ovens and refrigerators for storage, and centrifuges, balances, and hot plates for preparation and analysis. Glassware including pipettes, beakers, flasks, funnels, test tubes, petri dishes, and filter paper are also outlined. The purpose of each piece of equipment in laboratory research and testing is briefly explained.
This document provides summaries of several common laboratory techniques and procedures:
1. It describes how to properly measure volume using graduated cylinders and pipettes, and how to read meniscus levels.
2. It explains how to weigh samples using balances, including zeroing the balance and using tare functions.
3. Common separation techniques like decantation, filtration, centrifugation, distillation, and chromatography are summarized.
This document describes various pieces of lab equipment including beakers, Erlenmeyer flasks, volumetric flasks, graduated cylinders, test tubes, tongs, test tube holders, test tube brushes, scoopulas, thermometers, forceps, medicine droppers, test tube racks, stirring rods, Bunsen burners, retort stands, C-clamps, burettes, wire mesh, clay triangles, crucibles, funnels, filter paper, mortar and pestles, spot plates, evaporating dishes, Petri dishes, and watch glasses. Each item is briefly described in terms of its common laboratory uses and characteristics.
The document provides guidance for safely conducting laboratory experiments. It lists tips for laboratory set-up and procedures, such as assembling apparatus away from edges, following experimental procedures exactly unless instructed otherwise, avoiding distractions, reading labels carefully, and informing instructors of any accidents or injuries. Personal protective equipment like closed shoes and secured hair are required. Chemicals should be treated as dangerous, and consumption or misuse is forbidden. Proper documentation and cleanup are also emphasized.
This document provides a rubric for evaluating 3D atomic model projects created by students using Bhor's atomic model. The rubric assesses students on accuracy of the atomic model, creativity, design/materials used, and labeling. For an accurate model, the number of protons, neutrons, and electrons must match the assigned element. Creativity is judged based on uniqueness of design. Design and materials are evaluated on appropriateness and quality of construction. Labels on the model and information card must include the element name, symbol, atomic number, and mass.
The water bath is an
instrument used in the
laboratory for carrying out
serological, biomedical,
and pharmaceutical tests at
specific temperature ranges.
as a partial requirement for one of my subject for this semester
I would like you to view my presentation and comment as well
I will be very glad if you find my presentation interesting, or comment on how I can improve my craft, THANK YOU :)
Analytical balances are highly precise instruments used to determine small mass measurements. They can measure up to four decimal places of grams and are sensitive enough to detect changes as small as 0.0001 g. Due to their precision, they must be used in draft-shielded environments and powered by electricity. Analytical balances are used to weigh small quantities in the milligram range when great accuracy is required, such as pharmaceutical applications. Proper use requires leveling the balance, zeroing it, and either directly placing solid samples or taring containers for liquids or powders before taking measurements.
This document describes various types of laboratory equipment used in medical laboratories. It discusses microscopes for examining small organisms, incubators and autoclaves for sterilizing culture media and equipment, ovens and refrigerators for storage, and centrifuges, balances, and hot plates for preparation and analysis. Glassware including pipettes, beakers, flasks, funnels, test tubes, petri dishes, and filter paper are also outlined. The purpose of each piece of equipment in laboratory research and testing is briefly explained.
There are two main types of cells - prokaryotic and eukaryotic cells. Prokaryotic cells were the earliest form of life and lack a nucleus and organelles. Eukaryotic cells are larger and more complex, with a nucleus that contains DNA organized into chromosomes and membrane-bound organelles. The key differences are that prokaryotes lack a nucleus and organelles while eukaryotes have these structures.
Glassware apparatus and their uses(pdf)Science Equip
Glassware is commonly used in laboratories to contain and transport liquids. Beakers can hold fluids for mixing, heating, and reactions, though their volume measurements may be approximate. Funnels are used for pouring and filtration, with separatory funnels having features to prevent spillage. Pipets precisely measure fluid amounts, while volumetric flasks are used to create precise solutions by filling to an etched line. All glassware should be cleaned immediately after use.
This power point work describe about polar and nonn polar compounds and how to find it very easily and it also explain dipole moment and its calculation...this includes some workout problems
Atomic number, Mass number, Relative atomic mass and Atomic mass unitQazi GHAFOOR
The document discusses atomic number, mass number, and relative atomic mass. It states that the atomic number of an element equals the number of protons in its nucleus. The mass number is the sum of protons and neutrons. An example problem finds an atom has 92 protons and 146 neutrons from a given mass number of 238 and atomic number of 92. Relative atomic mass is the average mass of an atom's isotope compared to carbon-12, measured in atomic mass units.
The document discusses cell theory and the history of cell discovery. It explains that Robert Hooke first observed cells in 1665 using a microscope. Anton van Leeuwenhoek later discovered single-celled organisms. In the 1830s-1840s, scientists including Matthias Schleiden, Theodor Schwann, and Rudolf Virchow developed cell theory, which states that all organisms are composed of cells, cells are the basic unit of life, and new cells are produced from existing cells. The document also describes key differences between prokaryotic and eukaryotic cells.
Laboratory apparatus and safety measuresjulielazarte
This document provides information about common laboratory equipment, their uses, and safety procedures. It lists and describes various pieces of equipment like beakers, Bunsen burners, burets, clay triangles, droppers, funnels, flasks, graduated cylinders, hot plates, pipets, ring stands, test tubes, holders, tongs, volumetric flasks, wash bottles, watch glasses, wire gauze, microscopes, balances, and fume hoods. It also outlines important safety measures and prohibited behaviors in the laboratory, such as wearing protective equipment, cleaning up, and reporting any accidents.
The document discusses different types of microscopes used to view microscopic specimens. It describes light microscopes, which use lenses and visible light, including brightfield, darkfield, phase contrast, and fluorescence microscopes. It also describes electron microscopes, which use electromagnetic lenses and electrons beams to view specimens, including transmission electron microscopes that pass electrons through thin specimens, and scanning electron microscopes that scan surfaces to produce 3D images. Key aspects and uses of each microscope type are outlined.
This document outlines safety guidelines and procedures for working in a clinical laboratory. It discusses proper personal protective equipment like laboratory coats and closed-toe shoes. Specific guidelines are provided for handling glassware, chemicals, waste and biohazards safely. Proper procedures are outlined for tasks like centrifuging, microscopy, pipetting and blood sample collection and handling to protect workers and ensure accurate results. Maintaining cleanliness and order in the laboratory is emphasized for safety.
Microscopes are instruments designed to produce magnified images of small objects. They must accomplish three tasks: produce a magnified image, separate details in the image, and render details visible. There are different types of microscopes including simple, compound, stereoscopic, electron, scanning electron, and transmission electron microscopes. Electron microscopes use a beam of electrons instead of light to magnify images and can achieve higher magnifications than light microscopes. Confocal laser scanning microscopes use a laser beam to generate 3D images of thick specimens.
This document discusses isotopes and atomic structure. It defines isotopes as atoms of the same element that have the same number of protons but different numbers of neutrons. Isotopes have the same chemical properties but slightly different physical properties. The document also discusses nucleon number, which is the total number of protons and neutrons in an atom's nucleus. The mass number equals the nucleon number. Radioactive isotopes are unstable and undergo nuclear decay over time into stable daughter isotopes.
The document discusses the benefits of exercise for mental health. Regular physical activity can help reduce anxiety and depression and improve mood and cognitive function. Exercise causes chemical changes in the brain that may help protect against mental illness and improve symptoms.
This document provides information about various pieces of laboratory equipment and apparatus. It describes 34 different types of laboratory apparatus, including beakers, test tubes, Florence flasks, Erlenmeyer flasks, graduated cylinders, funnels, evaporating dishes, crucibles, reagent bottles, Bunsen burners, clamps, stands, rings, test tube holders, brushes, gauzes, tripods, spatulas, stirring rods, mortar and pestles, droppers, racks, thermometers, microscopes, balances, pipettes, burettes, platforms, analytical balances, alcohol lamps, tongs, volumetric flasks, spring balances, and hot plates. It also provides safety guidelines for working in a laboratory
The document discusses compound light microscopes. It explains that compound microscopes use multiple lenses to magnify specimens and have different light sources - some use mirrors to focus sunlight while others use illuminators. It then describes the main parts of a compound microscope - the eyepiece, body tube, objectives, stage, arm, base, illuminator, nosepiece, condenser, and diaphragm. Finally, it discusses how light travels through the microscope and some common uses of compound microscopes, such as pathology, forensics, education, and biology.
This document provides an introduction to chemistry, including defining matter and its three states (solid, liquid, gas). It discusses the particles that compose matter (atoms, molecules, ions) and their properties. Methods for separating mixtures are also outlined, such as filtration, distillation, evaporation, and chromatography. Consumer products often contain mixtures to appeal to customers, with examples given of household cleaning supplies and personal care items.
The microscope and its historical developmentArmand Anthony
This document provides information on the historical development of the microscope from the 1600s to modern day. It describes key figures like Zacharias Janssen, Hans Janssen, Robert Hooke, and Anton van Leeuwenhoek who helped advance microscope technology. The text also outlines the basic parts of a compound light microscope like the eyepiece, revolving nosepiece, objectives, stage, diaphragm, light source, arm, coarse and fine adjustment knobs, and base. Proper use and storage procedures for microscopes are also outlined.
The document discusses electron configurations, which describe how electrons are distributed in atomic orbitals. It explains the Aufbau principle, which states that electrons fill lower energy orbitals first. The Pauli exclusion principle is described, stating that no more than two electrons can occupy any single orbital. Hund's rule is also covered, regarding the filling of degenerate orbitals. Examples are provided to illustrate these principles.
This document discusses key concepts related to solution concentration including:
- Solutions are homogeneous mixtures with a solvent as the major component and solute as the minor component.
- Concentration can be expressed in various ways such as percentage by weight or volume, molarity, and parts per million or billion.
- Dilute solutions have low concentrations while concentrated solutions have high concentrations.
- Molarity calculations allow converting between moles, liters, and grams of solutes and solvents in solutions.
- Dilution decreases concentration by adding more solvent while maintaining the same amount of solute.
Two Types Of Cells - Eukaryotic and Prokaryotic Cellssth215
There are two main types of cells: prokaryotic and eukaryotic cells. Prokaryotic cells do not have a nucleus and include bacteria, while eukaryotic cells do have a nucleus and include plants, animals, fungi and protists. The key difference between these cell types is the presence of a nucleus - prokaryotes lack a nucleus, while eukaryotes have membrane-bound nuclei that contain their genetic material. Eukaryotic cells are more complex with many organelles and can form multicellular organisms.
This document outlines basic lab safety principles and procedures. It discusses how lab safety must be taught to all employees, students, and visitors. Key safety topics covered include general procedures like prohibiting food/drink and working alone in the lab. Proper use and maintenance of glassware, equipment, and personal protective equipment is emphasized. The document also addresses safety equipment availability and use, chemical handling and storage, waste disposal procedures, and electrical safety. Laboratory policies and procedures should be written, site-specific, and reviewed when new employees, procedures, equipment or chemicals are introduced.
This document provides an overview of plant taxonomy and anatomy. It begins by outlining the major divisions of the plant kingdom from non-vascular plants like mosses to vascular plants with and without seeds. It then describes the distinguishing features of monocots and dicots. The remainder of the document details plant structures like roots, stems, leaves, flowers, fruits and seeds through diagrams and descriptions.
There are two main types of cells - prokaryotic and eukaryotic cells. Prokaryotic cells were the earliest form of life and lack a nucleus and organelles. Eukaryotic cells are larger and more complex, with a nucleus that contains DNA organized into chromosomes and membrane-bound organelles. The key differences are that prokaryotes lack a nucleus and organelles while eukaryotes have these structures.
Glassware apparatus and their uses(pdf)Science Equip
Glassware is commonly used in laboratories to contain and transport liquids. Beakers can hold fluids for mixing, heating, and reactions, though their volume measurements may be approximate. Funnels are used for pouring and filtration, with separatory funnels having features to prevent spillage. Pipets precisely measure fluid amounts, while volumetric flasks are used to create precise solutions by filling to an etched line. All glassware should be cleaned immediately after use.
This power point work describe about polar and nonn polar compounds and how to find it very easily and it also explain dipole moment and its calculation...this includes some workout problems
Atomic number, Mass number, Relative atomic mass and Atomic mass unitQazi GHAFOOR
The document discusses atomic number, mass number, and relative atomic mass. It states that the atomic number of an element equals the number of protons in its nucleus. The mass number is the sum of protons and neutrons. An example problem finds an atom has 92 protons and 146 neutrons from a given mass number of 238 and atomic number of 92. Relative atomic mass is the average mass of an atom's isotope compared to carbon-12, measured in atomic mass units.
The document discusses cell theory and the history of cell discovery. It explains that Robert Hooke first observed cells in 1665 using a microscope. Anton van Leeuwenhoek later discovered single-celled organisms. In the 1830s-1840s, scientists including Matthias Schleiden, Theodor Schwann, and Rudolf Virchow developed cell theory, which states that all organisms are composed of cells, cells are the basic unit of life, and new cells are produced from existing cells. The document also describes key differences between prokaryotic and eukaryotic cells.
Laboratory apparatus and safety measuresjulielazarte
This document provides information about common laboratory equipment, their uses, and safety procedures. It lists and describes various pieces of equipment like beakers, Bunsen burners, burets, clay triangles, droppers, funnels, flasks, graduated cylinders, hot plates, pipets, ring stands, test tubes, holders, tongs, volumetric flasks, wash bottles, watch glasses, wire gauze, microscopes, balances, and fume hoods. It also outlines important safety measures and prohibited behaviors in the laboratory, such as wearing protective equipment, cleaning up, and reporting any accidents.
The document discusses different types of microscopes used to view microscopic specimens. It describes light microscopes, which use lenses and visible light, including brightfield, darkfield, phase contrast, and fluorescence microscopes. It also describes electron microscopes, which use electromagnetic lenses and electrons beams to view specimens, including transmission electron microscopes that pass electrons through thin specimens, and scanning electron microscopes that scan surfaces to produce 3D images. Key aspects and uses of each microscope type are outlined.
This document outlines safety guidelines and procedures for working in a clinical laboratory. It discusses proper personal protective equipment like laboratory coats and closed-toe shoes. Specific guidelines are provided for handling glassware, chemicals, waste and biohazards safely. Proper procedures are outlined for tasks like centrifuging, microscopy, pipetting and blood sample collection and handling to protect workers and ensure accurate results. Maintaining cleanliness and order in the laboratory is emphasized for safety.
Microscopes are instruments designed to produce magnified images of small objects. They must accomplish three tasks: produce a magnified image, separate details in the image, and render details visible. There are different types of microscopes including simple, compound, stereoscopic, electron, scanning electron, and transmission electron microscopes. Electron microscopes use a beam of electrons instead of light to magnify images and can achieve higher magnifications than light microscopes. Confocal laser scanning microscopes use a laser beam to generate 3D images of thick specimens.
This document discusses isotopes and atomic structure. It defines isotopes as atoms of the same element that have the same number of protons but different numbers of neutrons. Isotopes have the same chemical properties but slightly different physical properties. The document also discusses nucleon number, which is the total number of protons and neutrons in an atom's nucleus. The mass number equals the nucleon number. Radioactive isotopes are unstable and undergo nuclear decay over time into stable daughter isotopes.
The document discusses the benefits of exercise for mental health. Regular physical activity can help reduce anxiety and depression and improve mood and cognitive function. Exercise causes chemical changes in the brain that may help protect against mental illness and improve symptoms.
This document provides information about various pieces of laboratory equipment and apparatus. It describes 34 different types of laboratory apparatus, including beakers, test tubes, Florence flasks, Erlenmeyer flasks, graduated cylinders, funnels, evaporating dishes, crucibles, reagent bottles, Bunsen burners, clamps, stands, rings, test tube holders, brushes, gauzes, tripods, spatulas, stirring rods, mortar and pestles, droppers, racks, thermometers, microscopes, balances, pipettes, burettes, platforms, analytical balances, alcohol lamps, tongs, volumetric flasks, spring balances, and hot plates. It also provides safety guidelines for working in a laboratory
The document discusses compound light microscopes. It explains that compound microscopes use multiple lenses to magnify specimens and have different light sources - some use mirrors to focus sunlight while others use illuminators. It then describes the main parts of a compound microscope - the eyepiece, body tube, objectives, stage, arm, base, illuminator, nosepiece, condenser, and diaphragm. Finally, it discusses how light travels through the microscope and some common uses of compound microscopes, such as pathology, forensics, education, and biology.
This document provides an introduction to chemistry, including defining matter and its three states (solid, liquid, gas). It discusses the particles that compose matter (atoms, molecules, ions) and their properties. Methods for separating mixtures are also outlined, such as filtration, distillation, evaporation, and chromatography. Consumer products often contain mixtures to appeal to customers, with examples given of household cleaning supplies and personal care items.
The microscope and its historical developmentArmand Anthony
This document provides information on the historical development of the microscope from the 1600s to modern day. It describes key figures like Zacharias Janssen, Hans Janssen, Robert Hooke, and Anton van Leeuwenhoek who helped advance microscope technology. The text also outlines the basic parts of a compound light microscope like the eyepiece, revolving nosepiece, objectives, stage, diaphragm, light source, arm, coarse and fine adjustment knobs, and base. Proper use and storage procedures for microscopes are also outlined.
The document discusses electron configurations, which describe how electrons are distributed in atomic orbitals. It explains the Aufbau principle, which states that electrons fill lower energy orbitals first. The Pauli exclusion principle is described, stating that no more than two electrons can occupy any single orbital. Hund's rule is also covered, regarding the filling of degenerate orbitals. Examples are provided to illustrate these principles.
This document discusses key concepts related to solution concentration including:
- Solutions are homogeneous mixtures with a solvent as the major component and solute as the minor component.
- Concentration can be expressed in various ways such as percentage by weight or volume, molarity, and parts per million or billion.
- Dilute solutions have low concentrations while concentrated solutions have high concentrations.
- Molarity calculations allow converting between moles, liters, and grams of solutes and solvents in solutions.
- Dilution decreases concentration by adding more solvent while maintaining the same amount of solute.
Two Types Of Cells - Eukaryotic and Prokaryotic Cellssth215
There are two main types of cells: prokaryotic and eukaryotic cells. Prokaryotic cells do not have a nucleus and include bacteria, while eukaryotic cells do have a nucleus and include plants, animals, fungi and protists. The key difference between these cell types is the presence of a nucleus - prokaryotes lack a nucleus, while eukaryotes have membrane-bound nuclei that contain their genetic material. Eukaryotic cells are more complex with many organelles and can form multicellular organisms.
This document outlines basic lab safety principles and procedures. It discusses how lab safety must be taught to all employees, students, and visitors. Key safety topics covered include general procedures like prohibiting food/drink and working alone in the lab. Proper use and maintenance of glassware, equipment, and personal protective equipment is emphasized. The document also addresses safety equipment availability and use, chemical handling and storage, waste disposal procedures, and electrical safety. Laboratory policies and procedures should be written, site-specific, and reviewed when new employees, procedures, equipment or chemicals are introduced.
This document provides an overview of plant taxonomy and anatomy. It begins by outlining the major divisions of the plant kingdom from non-vascular plants like mosses to vascular plants with and without seeds. It then describes the distinguishing features of monocots and dicots. The remainder of the document details plant structures like roots, stems, leaves, flowers, fruits and seeds through diagrams and descriptions.
The impact of bullying in adolescents on schoolDenni Domingo
This document is a research paper on the impact of bullying in adolescents at school. It was submitted by 6 students to their professor and discusses several key points about bullying:
1. Bullying is a widespread problem that affects millions of students worldwide and can have both short-term and long-term negative consequences for both bullies and their victims.
2. There are different types of bullying, including physical, verbal, cyber, and emotional bullying. Bullies often target victims due to perceived differences in appearance, race, religion, nationality or other factors.
3. Bullying can seriously impact a person's mental health, self-esteem and academic performance. Both bullies and their victims are more likely to
(Science) Laboratory Operations and Techniquesjustinesolano
The document provides instructions for proper laboratory techniques including transferring solids and liquids, heating substances, separating solids from liquids through filtration and decantation, making saturated solutions, and determining odors. Students are to demonstrate these techniques precisely to avoid accidents and work efficiently in the lab. Key steps include using paper to transfer solids to test tubes without contamination, employing funnels or rods for precise liquid transfers, heating at angles without looking into tubes, allowing solids to settle before decanting or filtering mixtures, and cautiously sniffing odors from a distance.
The document discusses the background and objectives of using video surveillance systems in schools. It provides context on the history and development of surveillance cameras. Specifically, it outlines safety concerns in schools that surveillance could address, like the example of a shooting at a Philippine university. The conceptual framework shows installing surveillance equipment as an input, monitoring and analyzing footage as the process, and creating a safer learning environment as the output. Overall the document introduces the topic of incorporating surveillance technologies into school security systems.
This document provides an example of a technology use survey with 5 questions. It includes a table to record the responses from 5 students. The questions ask about daily text messages, feelings about Facebook, main mobile phone uses, favorite apps, and where photos are uploaded. Students were asked to conduct this survey, record the responses, and write a short report on their findings.
The document discusses the structure and function of plant roots. It describes how roots anchor the plant, absorb water and nutrients, and store food. Roots systems vary in their morphology between dicots and monocots. Roots have several tissues including the epidermis, cortex, endodermis, pericycle and vascular system. The root tip contains an apical meristem that drives root growth. Root hairs significantly increase the surface area for absorption. The endodermis regulates the movement of water and nutrients into the vascular system. Roots undergo modifications to serve functions like storage or support.
Questionnaire on the use of technology in the classroomangiegum
The document is a questionnaire about technology use for a classroom. It contains 3 sections:
A) Attitudes toward technology measured on a Likert scale. B) Experience using the internet for various purposes like communication, information, entertainment. C) Familiarity with and interest in incorporating future technology trends like e-learning, mobile learning, virtual learning environments, blogs, etc. into studies.
1) Morphology is the study of the external features, arrangement, and relationships of plant organs. Roots grow underground and absorb water and minerals, anchoring the plant. Stems grow above ground and transport nutrients between roots and leaves.
2) Roots have various modifications including storage roots, prop roots, stilt roots, climbing roots, and parasitic roots. Stems also have modifications underground like bulbs, corms, rhizomes, and above ground like runners, stolons, and suckers.
3) Vegetative plant organs include roots, stems, and leaves. Roots have different root systems and serve functions of anchoring, absorption, and storage. Stems support leaves and
Writing Chapters 1, 2, 3 of the Capstone Project Proposal ManuscriptSheryl Satorre
This document provides guidance for writing chapters 1-3 of a research proposal. It discusses what makes a good proposal, including clear objectives, thorough research, and realistic plans. It also describes elements to include in each chapter, such as the research problem and context in chapter 1, a literature review in chapter 2, and technical background in chapter 3. Guidelines are provided for writing each section concisely and comprehensively.
The study examined the common problems faced by freshmen students at the University of the Visayas. Through surveys, the researchers found that the top problems were financial/money issues, difficulty balancing work and studies, depression from being away from home, and lack of interest in certain subjects. The researchers aimed to identify ways to help students overcome these challenges in order to encourage them in their studies and build self-confidence.
Questionnaire for the survey of electronics market(for school/college projects)Dan John
The document is a 15 question questionnaire about consumer electronics. It asks respondents for their name, age, gender and occupation. It then asks questions to gauge the respondent's satisfaction with consumer electronics brands, purchase history of electronic gadgets, preferences between brands like Samsung and Apple, and perceptions of Samsung's products and market position.
This document provides an overview of a study on the effects of bullying among high school students. It includes an introduction to bullying, a literature review on the topic, and an outline of the study's methodology. The study aims to understand the profile of bullied students, identify the causes and effects of bullying, and make recommendations. It will use a descriptive research method and questionnaire to collect data from high school students in Cauayan City, Isabela, Philippines. The results of the study seek to help students, parents, and educators address the issue of bullying.
This document discusses the morphology of plants. It describes the three basic plant organs as roots, stems, and leaves. For each organ, it outlines their characteristics, functions, types and modifications. For roots, it discusses the root cap, zones of a root, taproot vs fibrous root systems, and storage and support root modifications. For stems, it covers nodes, internodes, buds and their types, as well as underground, sub-aerial and aerial stem modifications. Finally, it outlines leaf structure including petioles, blades, epidermis, stomata and mesophyll, and describes buds and their locations.
The document appears to be a survey for an MBA research thesis investigating customer attitudes towards luxury brands and mobile phones among Pakistani youth. It contains a table for respondents to provide their name, age, gender, mobile number, education and indicate their level of agreement on various statements related to social needs, materialism, consumer attitudes, experiential needs, fashion trends, need for uniqueness, and conformity in relation to luxury brands and mobile phones. Respondents are asked to rate their level of agreement on a 5-point scale from strongly agree to strongly disagree. All information provided will be kept confidential.
The document is a questionnaire given to students to understand factors affecting mathematics performance. It asks students to rate themselves on interest in math, study habits, and extracurricular activities. It also asks students to rate their math teachers on personality traits, teaching skills, and use of instructional materials. The questionnaire uses a 5-point scale and collects both qualitative and quantitative data from students.
This document summarizes a research study on factors affecting mathematics performance of high school students at Laguna State Polytechnic University in the 2009-2010 academic year. The study examines student-related factors like interest in mathematics, study habits, and teacher-related factors such as personality traits, teaching skills, and instructional materials. It provides background information on the importance of mathematics and reviews previous related studies. The research methodology, data collection instruments, and statistical analysis plan are also outlined.
MICROTECHNIQUE Killing and fixation moduleAbdulsalm
This document provides instructions for preparing biological specimens for light microscopy. It discusses the key steps of sample collection, killing and fixation, dehydration, clearing, paraffin embedding, microtomy, staining, and observation. Specific fixation fluids, dehydration reagents, clearing agents, and staining methods are described. The goal is to preserve specimens while modifying properties like refractive index to allow examination under a light microscope.
HEMATOXYLIN AND EOSIN (H & E) Staining.pptxsandeep singh
This document provides information on Hematoxylin and Eosin (H&E) staining, which is a common staining technique used in pathology. It describes how H&E staining works by using hematoxylin dye to stain cell nuclei blue and eosin dye to stain other structures pink. The document outlines the standard procedure for H&E staining of paraffin embedded tissue sections and frozen sections. It also discusses considerations for staining, potential problems that can occur, and how to remedy staining issues.
Oligomer & the importance of Oligomer remover-------
Oligomer is a smaller chain length of polyester made up of two or more molecules of basic ethylene terephthlate i.e. dimer or trimer, produced as a side reaction during the manufacture of polyester. The Oligoester because of its smaller chain length is in the form of a fine powder which is entrapped is basic polymer and migrates to the surface during high temperature polyester dyeing.
Higher the depth more is the displacement of Oligomer to the fibre surface. Typically polyester fibres contain between 1.5-3.5% by mass of low molecular esters, the principal oligomer being cyclic tris (ethylene terephthalate) with smaller quantities of dimer, pentamer & other compounds. Approximately 0.1 % to 1.0 % of Oligomer is produced as a side reaction in the manufacture of basic polyester.
Oligomer & the importance of Oligomer remover, problems caused on its deposit, Removing Art, Solutions----
Gels and jellies are semisolid dosage forms used to deliver drugs. Gels contain a gelling agent that is dispersed in water, producing a jelly-like consistency. Common gelling agents include polymers, natural gums, and cellulose derivatives. Gels are classified as single-phase or two-phase based on distribution of the gelling agent. Jellies are transparent and less greasy than gels. Common gelling agents for jellies include tragacanth, sodium alginate, pectin, and gelatin. Key evaluation tests for gels and jellies include pH, drug content, viscosity, and spreadability.
Oligomer is a byproduct of polyester production made up of small chains of ethylene terephthalate molecules. It exists as a fine powder trapped in polyester fibers and migrates to the surface during dyeing. Approximately 0.1-1.0% of oligomer is produced and can cause issues like impaired spinning, deposits, and dull or uneven dyeing. Various post-treatment methods have been used to remove oligomers but often damage fibers or are ineffective against cyclic oligomers. New treatments are needed that can remove oligomers with minimal production interruptions and fiber damage.
Oligomer is a byproduct of polyester manufacturing made up of small chains of ethylene terephthalate molecules. It is difficult to remove from polyester fibers and can cause issues during dyeing and spinning such as duller shades, dye spots, and clogged machine parts. Various post-treatment methods have been used to remove oligomers but often damage fibers or are not fully effective. Altranol products are effective at keeping oligomers dispersed during dyeing and alkaline treatments to prevent redepositing and allow more to be removed from fibers and machines.
This document provides information on various histopathology staining techniques. It describes the steps for taking paraffin sections to water, dehydrating and clearing sections in xylene, blotting sections dry, and mounting sections. It also details procedures for Ziehl-Neelsen staining for acid-fast bacilli, Gram-Twort staining for bacteria, Periodic acid Schiff staining, Periodic acid Schiff/Alcian blue staining, and the buffered Congo red method for amyloid. Precise reagents and safety notes are included for each technique.
This document provides information about tissue processing for medical laboratory technology (MLT) students. It discusses the basic steps in manual and automatic tissue processing, which are dehydration, clearing, and impregnation. Dehydration removes water from tissue using graded alcohols like ethanol. Clearing removes dehydrating fluids using xylene or other agents. Impregnation infiltrates tissue with paraffin wax. Automatic processors complete the steps more quickly through constant agitation in multiple chambers containing processing chemicals. The goal of tissue processing is to remove water from tissues and replace it with paraffin wax for embedding and microscopic examination.
Do not drink or put chlorine water in your mouth or eyes. The document provides instructions for making a 0.5% chlorine solution from liquid bleach or 70% chlorine powder for cleaning and disinfecting surfaces. It explains how to measure and mix the bleach or powder with water, label the bucket, use and store the solution safely, and make a fresh solution daily.
Special stains useful in Microbiology laboratory9925752690
This document provides information on various special stains used to identify microorganisms and cellular structures under the microscope. It discusses stains used for flagella, metachromatic granules, spirochetes, Chlamydia, rickettsia, and fungi. Specific stains covered include Wright's Giemsa, Gram, acid-fast, silver, toluidine blue, calcofluor white, acridine orange, auramine phenol, and lactophenol cotton blue. Procedures for each stain are provided along with what structures or organisms they help identify.
Do not drink or put chlorine water in your mouth or eyes. The document provides instructions for making a 0.5% chlorine solution from liquid bleach or 70% chlorine powder for cleaning and disinfecting surfaces. Key steps include wearing protective equipment, mixing specific amounts of bleach or powder with water, stirring for 10 seconds, labeling the container, and making a fresh solution daily.
Aerosol or Pressurized package is defined as ―A system that depends on the power of a compressed gas or liquefied gas to expel the contents from the container.
This document summarizes the production of the antifungal drug griseofulvin via fermentation. Griseofulvin is produced by fermenting the fungus Penicillium griseofulvum in Czapek Dox medium. The fermentation broth is then processed through several steps including pre-treatment, filtration, extraction with acetone, decolorization, isolation, precipitation and purification to yield purified griseofulvin crystals. The overall process involves growing the fungus in a fermenter, recovering the griseofulvin from the broth through solvent extraction and purification, yielding the final drug product.
Introduction, History types, manufacturing, Machinery used, filling by manual and automatic machines, Formulation, quality control test, Shape and sizes, properties of gelatin etc
Staining ( rouine and special in cytology) rajiv kumarrajusehrawat
The document discusses staining techniques used in histology and cytology. It provides details on the preparation, components, and use of common stains including Hematoxylin, Giemsa stain, Papanicolaou stain, and Periodic acid–Schiff stain. The stains are used to differentially color structures like nuclei, cytoplasm, muscles, bones, parasites and glycogen under the microscope to enable examination of tissue samples and identification of cells and microorganisms.
This document provides instructions for making chlorine dilutions for disinfection and antisepsis. It discusses making 0.5% chlorine solution for cleaning surfaces and objects, 0.05% chlorine solution for hand washing, and eusol solution. To make 0.5% chlorine solution, 10 tablespoons of 70% chlorine powder are added to 20 liters of water. To make 0.05% chlorine solution, 100ml of the 0.5% solution is added to 900ml of water. Eusol is made by adding 1.25mg of chlorine powder and 1.25mg of boric acid to 100ml of distilled water to help remove slough and necrotic
This document provides instructions for performing a Ziehl-Neelsen stain to identify acid-fast bacteria, including Mycobacterium tuberculosis. The stain involves incubating tissue sections in carbol fuchsin, washing in acid alcohol to decolorize non-acid fast bacteria, and counterstaining with methylene blue. Acid-fast bacteria will appear bright red against a blue background. Proper use of reagents and safety precautions are described to safely identify these pathogens.
The document discusses Soxhlet extraction, which is a method of extracting compounds from solids using liquid solvents. It involves placing the solid in a thimble or filter paper inside an extraction chamber, and continuously washing it with solvent heated to its boiling point. The solvent extracts the desired compounds, then collects in a receiving flask. Key steps include selecting an appropriate solvent, drying plant materials, setting up the apparatus, running the extraction for several hours, and recovering the extract by evaporating the solvent. Advantages are high efficiency and yield, while disadvantages include length of time and potential thermal degradation of compounds.
A workshop hosted by the South African Journal of Science aimed at postgraduate students and early career researchers with little or no experience in writing and publishing journal articles.
The simplified electron and muon model, Oscillating Spacetime: The Foundation...RitikBhardwaj56
Discover the Simplified Electron and Muon Model: A New Wave-Based Approach to Understanding Particles delves into a groundbreaking theory that presents electrons and muons as rotating soliton waves within oscillating spacetime. Geared towards students, researchers, and science buffs, this book breaks down complex ideas into simple explanations. It covers topics such as electron waves, temporal dynamics, and the implications of this model on particle physics. With clear illustrations and easy-to-follow explanations, readers will gain a new outlook on the universe's fundamental nature.
A review of the growth of the Israel Genealogy Research Association Database Collection for the last 12 months. Our collection is now passed the 3 million mark and still growing. See which archives have contributed the most. See the different types of records we have, and which years have had records added. You can also see what we have for the future.
Strategies for Effective Upskilling is a presentation by Chinwendu Peace in a Your Skill Boost Masterclass organisation by the Excellence Foundation for South Sudan on 08th and 09th June 2024 from 1 PM to 3 PM on each day.
हिंदी वर्णमाला पीपीटी, hindi alphabet PPT presentation, hindi varnamala PPT, Hindi Varnamala pdf, हिंदी स्वर, हिंदी व्यंजन, sikhiye hindi varnmala, dr. mulla adam ali, hindi language and literature, hindi alphabet with drawing, hindi alphabet pdf, hindi varnamala for childrens, hindi language, hindi varnamala practice for kids, https://www.drmullaadamali.com
বাংলাদেশের অর্থনৈতিক সমীক্ষা ২০২৪ [Bangladesh Economic Review 2024 Bangla.pdf] কম্পিউটার , ট্যাব ও স্মার্ট ফোন ভার্সন সহ সম্পূর্ণ বাংলা ই-বুক বা pdf বই " সুচিপত্র ...বুকমার্ক মেনু 🔖 ও হাইপার লিংক মেনু 📝👆 যুক্ত ..
আমাদের সবার জন্য খুব খুব গুরুত্বপূর্ণ একটি বই ..বিসিএস, ব্যাংক, ইউনিভার্সিটি ভর্তি ও যে কোন প্রতিযোগিতা মূলক পরীক্ষার জন্য এর খুব ইম্পরট্যান্ট একটি বিষয় ...তাছাড়া বাংলাদেশের সাম্প্রতিক যে কোন ডাটা বা তথ্য এই বইতে পাবেন ...
তাই একজন নাগরিক হিসাবে এই তথ্য গুলো আপনার জানা প্রয়োজন ...।
বিসিএস ও ব্যাংক এর লিখিত পরীক্ষা ...+এছাড়া মাধ্যমিক ও উচ্চমাধ্যমিকের স্টুডেন্টদের জন্য অনেক কাজে আসবে ...
This presentation was provided by Steph Pollock of The American Psychological Association’s Journals Program, and Damita Snow, of The American Society of Civil Engineers (ASCE), for the initial session of NISO's 2024 Training Series "DEIA in the Scholarly Landscape." Session One: 'Setting Expectations: a DEIA Primer,' was held June 6, 2024.
How to Build a Module in Odoo 17 Using the Scaffold MethodCeline George
Odoo provides an option for creating a module by using a single line command. By using this command the user can make a whole structure of a module. It is very easy for a beginner to make a module. There is no need to make each file manually. This slide will show how to create a module using the scaffold method.
This presentation includes basic of PCOS their pathology and treatment and also Ayurveda correlation of PCOS and Ayurvedic line of treatment mentioned in classics.
3. Water will not mix with usual media in which stained
and sectioned materials may be finally preserved.
The purpose dehydration is therefore, to remove all
traces of water from the cells and tissues before finally
mounting.
Dehydration : treating the material with the series of
solutions containing progressively decreasing
concentration of water and progressively increasing
concentration of the dehydrant.
4. The material should not be allowed to become dry at
any stage .
Long interval in long concentrations make soft tissues
Long interval in high concentrations make materials
brittle.
In the last but one grade of dehydrant,a very small
quantity of eosin powder may be added to colour the
material dehydrated for embedding in paraffin, this
helps in locating materials.
6. DEHYDRATING AGENTS
Ethyl alcohol
Most commonly used.
Isopropyl alcohol is used instead of this
,it is cheap and easily produced
Rarely used with plam materials due to
high toxicity.
Drastic dehydrating action that damage
structures.
7. Strength of alcohol
60,70,80 percent alcohol by volume ,to be stored in
glass stoppered reagim-bottles ,next in this series will
be 95 % and absolute alcohol.
Normally the time for which materials are left in each
grade shall be 30 minutes for root tips and small pieces
of leaf ,12 hours for large blocks of wood
Up to 70% and 1 hr and 24hr respectively for 80,95,&
100% .
8. Acetone
The series to be prepared with distilled H2O are 75
,10,15,20,25,30,35,40,50,60,70,80,90,100 %
Intervals can be less than one hour in each grade
The procedure is exactly the same as with ethyl or
isopropyl alcohol.
9. GLYCERINE
It is a thick, colorless, sweet tasting liquid and is an
excellent reagent for dehydrating delicate materials
like filamentous algae and fungi
High boiling point
Water can be removed by evaporation.
Material is washed well and transferred to a large
volume of 5% glycerin take in a wide mouthed jar
Leave this jar open but well protected from dust in a
room until the water evaporates leaving the material
covered by glycerin
10. BUTYL ALCOHOL AND TERTIARY BUTYL
ALCOHOL
Have additional advantage that they can be directly
followed by paraffin impregnation since they are also
solvents of paraffin.
11. Different grades are prepared in combination with
ethyl alcohol and are tabulated below.
SERIES NO ETHYL
ALCOHOL
95% IN ml
NORMAL
BUTYL
ALCOHOL
IN ml
DISTILLED
WATER IN
ml
1 20 10 70
2 25 15 60
3 30 25 45
4 30 40 30
5 25 55 20
6 20 70 10
7 15 85 0
12. SERIES NO ABSOLUTE
ALCOHOL IN
ml
95%ETHYL
ALCOHOL
TERTIARY
BUTYL
ALCOHOL IN
ml
DISTILLED
WATER IN ml
1 0 50 10 40
2 0 50 20 30
3 0 50 35 15
4 0 50 50 0
5 25 0 75 0
The series with tertiary butyl alcohol
13. DIOXANE
DIOXAN (diethylene dioxide)
Does not require any other reagents to be mixed for
dehydration.
No drastic plasmolysing effect
Rapid penetration capacity
Does not make tissue brittle
Reduces the number of separate steps in the process of
dehydration
14. The following series are recommented for materials
washed in water fixing.
Dioxan series 1
SERIES NO DIOXAN IN
ml
DISTILLED
WATER IN
ml
DURATION
IN EACH IN
hr
1 35 65 4-12
2 60 35 4-12
3 100 0 4-12
SERIES NO DIOXANE
IN ml
DISTILLED
WATER IN
ml
DURATION
IN EACH IN
hr
1 50 50 4-12
2 65 35 4-12
3 100 0 4-12
15. Clearing or dealcoholization
Transfer of materials after dehydration in the reagents
that are not solvents of wax
The clearing action is merely incidental to the
function of the reagent to serve as solvent wax.
16. XYLENE (xylol)
a conventional reagent
Used for dealcoholization before materials transferred
to solvents of paraffin
Trichloroethylene is a good substitute for xylene ,it is
non toxic and non-inflammable.
17. Xylene/ trichloroethylene series for large pieces of
tissue
SERIES NO ETHYL
ALCOHOLI
N ml
XYLENE
/TRICHLOR
OETHYLEN
E IN ml
DURATION
IN HOUR
1 75 25 3
2 50 50 3
3 25 75 3
4 0 100 3
18. Xylene/ trichloroethylene for cytological work
SERIES NO ETHYL
ALCOHOL
IN ml
XYLENE/TR
ICHLOROE
THYLENE
IN ML
DURATION
IN min
1 90 10 30
2 80 20 30
3 70 30 30
4 60 40 30
5 50 50 30
6 40 60 30
7 30 70 30
8 20 80 30
9 10 90 30
10 0 100 30
19. CHLOROFORM
Does not make tissue brittle
Chloroform/benzene series.
SERIAL NO ETHYL
ALCOHOL
CHLOROFO
RM IN ml
DURATION
IN Hr
1 65 35 ½-3
2 35 65 ½-3
3 0 100 ½-3
Benzene is a good substitute in this
series
Chloroform and benzene vapors are
inflammable and harmful in inhale too
much.
20. CEDER OIL
Used like xylol.
Easy to obtain but clearing action is low.
CLOVE OIL
Obtain from syzyginum aromaticum.
Eugenol is the major constituent.
Used to clear sections in the slides before mounting.
Give better transparency to tissues & hence visible
clearly .
21. BEECH WOOD CREOSOTE
fragile materials are dehydrated using creosote from
80% alcohol
2 changes are enough to have complete dehydration
Later mounting is done using balsam .
It has method of clearing.
Counter staining can also be done
Fern prothallus ,sections of bryophyte thallus etc…are
double stained properly using creosote method.