This document summarizes a presentation on skin and soft tissue infections (SSTIs). SSTIs can be caused by bacteria, fungi, parasites, or viruses. They range from mild to life-threatening. Risk factors include hospitalization, skin injuries, surgery, skin conditions like eczema, obesity, diabetes, and immunosuppression. Classification is based on lesion type, causative agent, and pathogenesis. Clinical manifestations vary and include dermatitis, erythrasma, ringworm, pyoderma, impetigo, and necrotizing infections. Diagnosis involves specimen collection and testing like microscopy, culture, identification and antimicrobial susceptibility. Treatment is guided by test results and prevention focuses on hygiene,
This presentation includes introduction, properties, transmission, epidemiology, pathogenesis, mechanism of infection, immunity and hypersensitivity, clinical manifestations, diagnosis, treatment, prevention and control of MYCOBACTERIUM TUBERCULOSIS.
Fungal infection of the skin, most common on the exposed surfaces of the body, namely the face, arms and shoulders.
Most common fungal diseases ; Ringworm. A common fungal skin infection that often looks like a circular rash.
This presentation includes introduction, properties, transmission, epidemiology, pathogenesis, mechanism of infection, immunity and hypersensitivity, clinical manifestations, diagnosis, treatment, prevention and control of MYCOBACTERIUM TUBERCULOSIS.
Fungal infection of the skin, most common on the exposed surfaces of the body, namely the face, arms and shoulders.
Most common fungal diseases ; Ringworm. A common fungal skin infection that often looks like a circular rash.
SKIN,SOFT TISSUE AND JOINTS INFECTIONS_07Dec2021.pptxMkindi Mkindi
There are nearly 100 viruses of the herpes group that infect many different animal species.
Official name of herpesviruses that commonly infect human is Humans herpesvirus (HHV)
herpes simplex virus types 1 (HHV 1)
Herpes simplex virus type 2 (HHV 2)
Varicella-zoster virus (HHV 3)
Epstein-Barr virus, (HHV 4)
Cytomegalovirus (HHV 5)
Human herpesvirus 6 (HHV 6)
Human herpesvirus 7 (HHV 7)
Human herpesvirus 8 (HHV 8) (Kaposi's sarcoma-associated herpesvirus).
Herpes B virus of monkeys can also infect humans
hELMINTHS#corona virus#Aspergillosis#BUGANDO#CUHAS#CUHAS#CUHAS
mucoromycosis is a fungal infection mostly caused by saprophytic molds.
these fungi are found widely in environment, mostly in damp areas or areas with good carbon source, as carbon is essential or basic needs for such fungi.
their infection is categorised into three categories,
cutaneous mucoromycosis,
sub-cutaneous mucoromycosis,
disseminated mucoromycosis.
this ppt will gives you an overview regarding mucoromycosis its causative agent, parthenogenesis and lab diagnosis and treatment.
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
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- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
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micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
Anti ulcer drugs and their Advance pharmacology ||
Anti-ulcer drugs are medications used to prevent and treat ulcers in the stomach and upper part of the small intestine (duodenal ulcers). These ulcers are often caused by an imbalance between stomach acid and the mucosal lining, which protects the stomach lining.
||Scope: Overview of various classes of anti-ulcer drugs, their mechanisms of action, indications, side effects, and clinical considerations.
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
MANAGEMENT OF ATRIOVENTRICULAR CONDUCTION BLOCK.pdfJim Jacob Roy
Cardiac conduction defects can occur due to various causes.
Atrioventricular conduction blocks ( AV blocks ) are classified into 3 types.
This document describes the acute management of AV block.
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...
L8. Skin and soft tissue infections .pptx
1. SKIN AND SOFT TISSUE INFECTIONS
DEPARTMENT OF MICROBIOLOGY AND IMMUNOLOGY
Presenter: Daniel Mwandu
Facilitator: Dr. Joel Manyahi
Date: 20th Dec, 2023
Venue: Microbiology Lab
3. INTRODUCTION
• Skin and soft tissue infections (SSTIs) are clinical
entities of variable presentation, etiology and severity
that involve microbial invasion of the layers of the skin
and underlying soft tissues.
• It can be caused by direct penetration or by
hematogenous spread of pathogens from initial sites
• SSTIs range from mild infections, such as pyoderma,
to serious life-threatening infections, such as
necrotizing fasciitis.
5. RISK FACTOR FOR SSTIS
• Long hospital stay
• Skin traumatic , Injury and open wounds
• Surgical,
• Skin conditions, such as athlete’s foot or eczema,
• Obesity, Diabetes,
• Immunodeficiency,
• Certain medications.
7. CLASSIFICATION
The skin and soft tissue infections, can be
classified according to
i. The type of skin lesion produced( superficial or
deep)
ii. The causative organism(bacteria, parasites
,fungi or Viruses)
iii. The pathogenesis of the infection such as a
primary entity or secondary to a preexisting
infection or systemic manifestation).
8. Clinical Manifestation
Dermatitis
• It is a general term that describes an inflammation
of the skin.
• It is characterized by areas of redness, swelling,
and sometimes scaling of the skin and pruritus.
• The common infectious causes of dermatitis.
Intertrigo and Superficial Candidiasis
9.
10. Cont..
Erythrasma
• It is a superficial, chronic skin infection
• Usually found in intertriginous areas
• It characterized by Red or brown
hyperpigmented patches of skin
with scaling and central
hypopigmentation.
• It often occurs in men, Obesity, DM
patients, and immunosuppressed.
• Corynebacterium minutissimum, a
skin biota, the causative organism,
produce a lesions with a coral red
fluorescence under a Wood lamp.
11.
12. Cont..
Dermatophytosis
• Dermatophytosis is an infection of the hair, skin,
or nails caused by a dermatophyte
• Three genus Trichophyton, Epidermophyton,
and Microsporum are known causative of
infection, also known as ringworm or tinea
• The classic lesion of a dermatophyte infection is
a circular scaly patch of erythema with a raised
border. The edges are often more inflamed than
the center.
16. Bite Infections
• Bites from humans or animals can result to serious
infections with a mixture of aerobic and anaerobic
organisms originated from biting oral cavity and skin biota
of the patient.
• Dog bites are typically polymicrobial and Pasteurella,
Bacteroides spp., Fusobacterium, Prevotella,
staphylococci, etc
• Cat teeth can inflict very deep wounds and have a greater
risk of infection, soft tissue abscess formation, and
infection of underlying bones and joints compared with dog
bites.
17. • Cat bites also progress to
infection more rapidly than dog
bites. Pathogens are similar to
dog with inclusion of Francisella
tularensis
• Human bite pathogens
infections include
Streptococcus anginosus
group, S. aureus, Eikenella
corrodens, Fusobacterium
nucleatum, and Prevotella
melaninogenica.
18. Cont..
Diabetic Foot Infections
• It is a very common in DM patients;
• It is risk factors including peripheral neuropathy,
traumatic feet, and kidney dysfunction.
• it can manifest to cellulitis, soft tissue
ulceration and gangrene
• Ulcerative lesions and gangrene may be is a
result of mixed infections of gram positive and
negative bacteria and both aerobes anaerobic
bacteria.
.
19. Necrotizing Soft Tissue Infection
• A necrotizing soft tissue infection is a serious,
life-threatening condition. It can destroy skin,
muscle, and other soft tissues.
• Subtypes of necrotizing infection (infectious
gangrene) include
i. Type I caused by polymicrobial
ii. Type II, monomicrobial usually S. pyogenes
iii. Gas gangrene (type III), caused by Clostridium
spp. and marine vibrios
21. Laboratory diagnosis
Specimen collection:
-Proper container
-Proper transport media
-Proper storage
conditions
Collection technique:
-deep pus swab
-needle Aspirate
Near skin swab
Diagnosis technique:
-Macroscopic
-Microscopic
-Culture and
sensitivity
Successful
diagnosis
22. Sample
• A variety of diagnostic methods may be helpful
in determining the cause of skin and soft tissue
infections.
• Swabs of surface wounds or skin are likely to
yield colonizing or contaminating bacteria.
• Therefore deep aspirates or biopsies are
recommended.
• Other specimen are Blood for culture (if systemic
infection is suspected) and Fluid aspirates from
infected lesion for culture
23.
24. Specimen collection;
• Collect the specimen using a sterile cotton-wool
swab if aspirate is not possible
• Pus from an abscess is best collected during
abscess incision and drainage.
• Pus from a wound should be collected before an
antiseptic dressing is applied
• Swabs should be well soaked in pus to collect
adequate pus.
24
26. Storage and transportation;
• If pus swab use Amies transport
medium.
• If aspirate transfer the fluid to a
sterile, leak-proof container.
• cooked meat medium (or
thioglycollate broth) when anaerobic
pathogen are suspected
27. Macroscopic examination
• Observing the presence of granules and
branching filaments suggestive of infections
with actinomycetes or fungi.
• Color- white-yellow, brown, green
• Smell eg foul smelling for Anaerobic infections
• a Wood lamp, for suspicion of dermatophytes
(Microsporum) will fluoresce yellow-green.
28. Microscopic examination
• Gram stain
• For fungi suspicion ,a wet mount with 10% to
20% potassium hydroxide solution. Also
Calcofluor white (CW) stain may also be used.
• if mycobacterial disease is suspected, ZN or FM
• To identify Nocardia species, a modified acid-
fast stain can be performed,
29. Culture
• Bacteria culture by using BA and CA, and MCA;
• For anaerobic organisms, an anaerobic transport
and growth media should be used to maximize
recovery.
• For fungal(Candida spp.) on Sabouraud dextrose
agar(SDA).
• For mycobacteria spp, Lowenstein-Jensen and
Middlebrook media could be used
• For viruses, cell culture to observe CPE.
30. Identification
• A Gram stain provides the morphologic
• Growth characteristics and colonies
appearance in culture media,
• Convention Biochemical tests and API 20E can
help identify the organism,
• An automated MALDI-TOF MS has recently
been adapted in many laboratories.
31. Other techniques. These include
• Urine antigen detection (e.g., for systemic fungi);
• serum antibody tests for bacteria, viruses, and
parasites;
• Immunological assay(ELISA)
• Molecular techniques(PCR) for the detection of
a great variety of bacteria, fungi, and viruses.
32. Antimicrobial susceptibility testing
• Antimicrobial susceptibility testing is
subsequently performed on isolates based on
Standards updated guidelines eg CLSI and
EUCAST.
• Also molecular techniquies can be used for
detection the mecA gene confer for methicillin
resistance (e.g., in S. aureus) and the vanA and
vanB genes conferring vancomycin resistance
(e.g., in Enterococcus).
• Also MALDI-TOF MS can be used
33.
34. Treatment,
• Treatment should be guided by AST results
• For bacteria wound dressing with mupirocin
(topical)
• For severe bacteria infection, Topically or orally
administered erythromycin or clindamycin and
amoxicillin-clavulanic are useful
• Superficial mycoses use topical antifungal
agents such as clotrimazole. Oral antifungal
agents such as fluconazole,
35. Prevention and control
Improve personal hygiene
Hand washing
Wash lesions with soap and water
Remove crust
Vaccination to immunocompromised such as
namely on conjugated vaccines against
pneumococcus, H influenzae and N meningitidis,
36. References
• Jawetz, Medical Microbiology, 28th Edition.
• Textbook of Diagnostic Microbiology-sixth Edition (2018)
By Connie R. Mahon, Donald C. Lehman.
• Monica Cheersburgh 2nd Edition.
• Tanzania Standard Treatment Guideline, 2021
• Published Articles.
Editor's Notes
The skin, skin structures, and normal microbiota play a significant role in protecting the host against microbial invasion and disease. ■ Virulence factors of disease-producing organisms (e.g., toxins) can enable the organisms to evade host defense mechanisms, which can result in severe manifestations of infection. ■ A compromised immune system can lead to more severe or unusual manifestations of infection and can allow normally innocuous organisms to be pathogenic. ■ The occurrence of disease in a host is a function of the underlying host’s immunity and virulence of the pathogen. ■ The method and site of collection, quality of the clinical specimen, and clinical context are all important factors to consider when distinguishing between colonization and infection. ■ Proper specimen collection and laboratory processing of specimens are factors critical to the success of making a microbiological diagnosis of infection
Bacteria, viruses, fungi, and parasites are all important causes of skin and soft tissue infections.
S. aureus and S. pyogenes are important causes of pyoderma.
Intertrigo (intertriginous dermatitis) is an inflammatory cutaneous condition that occurs in body areas subjected to heat, moisture, and friction, which work together to cause maceration and skin breakdown. Infectious agents enhance this process. Intertrigo usually occurs in the skin folds of infants and obese adults and often can be found in the axillae, in perineum (e.g., diaper rash), beneath the breasts, and in abdominal folds. The most common organism present in these areas is Candida, although S. aureus and coliforms also can play a role
A and B Axilla of a 65-year-old White man with erythrasma showing a well-demarcated erythematous plaque with fine scale (A). Wood lamp examination of the area showed characteristic bright coral red fluorescence (B).
C and D A well-demarcated, red-brown plaque with fine scale in the antecubital fossa of an obese Hispanic woman (C). Wood lamp examination revealed bright coral red fluorescence (D).
Transmitted by human contact, sharing of clothesi ans coms, also zoonotic.
Erysipelas
The impairment of host defenses seen in diabetic patients can also allow weakly virulent organisms, such as coagulase-negative staphylococci and diphtheroids, to be pathogens in the skin.
For example, if pustules or vesicles are present, the roof or crust should be removed with a sterile blade, and any pus or exudate should be Gram stained and cultured
Anaerobic culture
When an anaerobic infection is suspected (specimen is often foul-smelling), or the Gram smear shows an ‘anaerobic mixed flora’, inoculate a second blood agar plate and incubate it anaerobically (see subunit 7.4) for up to 48 hours. The anaerobic blood agar plate may be made selective by adding neomycin to it (see No. 16).
At a final neomycin concentration of 50–70 g/ml, the majority of facultative anaerobic Gram negative rods will be inhibited.