This document summarizes a study investigating the tribological properties of cotton seed oil with the addition of MoS2 and SiO2 nanoparticles. The study used a four-ball tester to measure the coefficient of friction and wear scar diameter of cotton seed oil samples with varying concentrations of MoS2 and SiO2 additives. The results showed that adding 1% SiO2 nanoparticles reduced the coefficient of friction and wear scar diameter the most compared to other additive concentrations. Therefore, SiO2 nanoparticles show potential for improving the lubricating and anti-wear properties of cotton seed oil.