The document discusses insulin, including its structure, gene location, processing in the body, and treatment of diseases related to insulin deficiency. Insulin is produced in the pancreas and enables cells to take up glucose. The human insulin gene is located on chromosome 11. Insulin is composed of two peptide chains linked by disulfide bonds. Treatment for insulin deficiency involves injection of insulin extracted from animals or recombinant human insulin produced using biotechnology. Recombinant human insulin is made through bacterial or yeast expression systems using vectors like plasmids or yeast artificial chromosomes.
Introduction :
Antibiotics are antimicrobial agents produced naturally by other microbes (usually fungi or bacteria)
The first antibiotic was discovered in 1896 by Ernest Duchesne and in 1928 "rediscovered" by Alexander Fleming from the filamentous fungus Penicilium notatum.
The antibiotic substance, named penicillin, was not purified until the 1940s (by Florey and Chain), just in time to be used at the end of the second world war.
Penicillin was the first important commercial product produced by an aerobic, submerged fermentation
Introduction
Definition
History
Why are the transgenic animals being produced
Transgenic mice
Mice: as model organism
Methods of creation of transgenic mice
knock-out mice
Application of transgenic mice
Conclusion
References
Introduction :
Antibiotics are antimicrobial agents produced naturally by other microbes (usually fungi or bacteria)
The first antibiotic was discovered in 1896 by Ernest Duchesne and in 1928 "rediscovered" by Alexander Fleming from the filamentous fungus Penicilium notatum.
The antibiotic substance, named penicillin, was not purified until the 1940s (by Florey and Chain), just in time to be used at the end of the second world war.
Penicillin was the first important commercial product produced by an aerobic, submerged fermentation
Introduction
Definition
History
Why are the transgenic animals being produced
Transgenic mice
Mice: as model organism
Methods of creation of transgenic mice
knock-out mice
Application of transgenic mice
Conclusion
References
This presentation is about what exactly is penicillin and how it was discovered along with its industrial production process from fermentors until yield.
penicillins - power point - History,mechanism of action,classification,chemis...Dr. Ravi Sankar
Antibiotics - Penicillin's - power point - History, mechanism of action, classification, chemistry, SAR, Nomenclature, uses, side effects- Medicinal chemistry.
Prof. P. Ravisankar M. Pharm., Ph.D.
HOD .,
Vignan Pharmacy college
vadlamudi- Guntur-A.P, India.
banuman35@gmail.com
Phone: 0 9059994000
0 9000199106
Explanation on the industrial production of penicillin covering the history, fermentors, specific conditions required for penicillin production, how to increase yield amongst others.
Antibiotics,antibiotics resistances,classification of antibiotics,misuse of antibiotics details discussed here. for more information visit my blog helpful for pharmacy and medical student.thanks.
http://mydreamlan.wordpress.com/category/education/
myjunior09.wordpress.com
CONTENTS-
Introduction
• History
• SCP production in India
• Raw materials
• SCP production
• Advantages and Disadvantages
• Applications
• Conclusion
• References
The 1st recombinant drug .
A protein chain or peptide hormone.
A dimer of an A-chain & a B-chain linked together by disulfide bonds, composed of 110 aa & molecular mass is 5808 Da.
A product of commercially important fermentation process that produce recombinant products.
Naturally produced by beta cells of the islets of Langerhans in the pancreas & by Brockmann body in some teleost fish.
The preproinsulin precursor of insulin is encoded by the INS gene.
Important for metabolism and utilization of energy from the ingested nutrients – especially glucose.
Failure of control of insulin level causes diabetes mellitus.
Synthetic Biology for Plant ScientistsSachin Rawat
Tools of synthetic biology can be utilised to engineer metabolic pathways to optimize production of secondary metabolites and ligno-cellulose. The presentation describes an approach to develop an artificial positive feedback loop to increase accumulation of cell wall polysaccharides. These will decrease the cost of production of plant-based biofuels, paper and other plant products.
Immobilization is "the imprisonment of an enzyme in a distinct phase that allows exchange with, but is separated from the bulk phase in which the substrate, effector or inhibitor molecules are dispersed and monitored"
Plants are natural sources of valuable secondary metabolites used in pharmaceuticals, agrochemicals, the food industry, etc.
There is an increasing demand to obtain these metabolites through more productive plant tissue applications and cell culture methods.
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
Acute scrotum is a general term referring to an emergency condition affecting the contents or the wall of the scrotum.
There are a number of conditions that present acutely, predominantly with pain and/or swelling
A careful and detailed history and examination, and in some cases, investigations allow differentiation between these diagnoses. A prompt diagnosis is essential as the patient may require urgent surgical intervention
Testicular torsion refers to twisting of the spermatic cord, causing ischaemia of the testicle.
Testicular torsion results from inadequate fixation of the testis to the tunica vaginalis producing ischemia from reduced arterial inflow and venous outflow obstruction.
The prevalence of testicular torsion in adult patients hospitalized with acute scrotal pain is approximately 25 to 50 percent
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
Knee anatomy and clinical tests 2024.pdfvimalpl1234
This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
3. • Introduction
• Structure of the insulin
• Insulin gene
• Insulin processing in the body
• Disease
• Treatment
• Recombinant Insulin and process
• Host cells
• Vectors
• Bioreactors
4. INSULIN
• Is a polypeptide hormone produced
by the β cells of the islets of
Langerhans in the pancreas
• Its main function is enabling the
cells to take up glucose ( providing
it with energy it needs).
• It is required for normal glucose
homeostasis.
7. Which Chromosome Insulin Gene Is
Found On?
• INS: Insulin gene is found on
chromosome (11).
• It is found on the short arm of
chromosome 11 ( p arm).
• It is located region 1 , band 5, sub-
band 5.
8. The Structure Of Insulin
• Insulin is composed of two peptide
chains referred to as the α chain and
β chain.
• α and βchains are linked together by
two disulfide bonds, and an
additional disulfide is formed within
the α chain.
• α chain consists of 21 amino acids
and the β chain of 30 amino acids.
12. Importance Of Insulin
• Without insulin, the blood glucose builds up in the blood and the cells
are starved of their energy source.
• Some of the symptoms that may occur include fatigue, constant
infections, blurred eye sight, numbness, tingling in the hands or legs,
increased thirst, and slowed healing of bruises or cuts.
• The cells will begin to use fat, the energy source stored for
emergencies.
• When this happens for too long a time the body produces ketones,
chemicals produced by the liver.
• Ketones can poison and kill cells if they build up in the body over an
extended period of time. This can lead to serious illness and coma.
13.
14.
15. TREATMENT
• Banting and Best invented the insulin hormone in 1921.
• Several individuals died since their glucose level increased due to
disorder of insulin production .
• Treatment via insulin injection extracted from animal
pancreas.
16. PROBLEM?
Animal Insulin swine and cow.
Synthetic Human Insulin Slight difference in composition
As a consequence
• Human body produces antibodies against the animal insulin;
inflammation at the area of injection.
• Long-term complications occurred
• Decrease in animal insulin production
22. ADVANTAGES OF USING E.COLI AS A
SYSTEM
• Simple, well-understood genetics
• Ease of genetic manipulation
• Minimal culturing cost
• Fast expression (doubling time is only 20 - 30 mins).
• Well established labeling protocols for stability studies.
• Established regulatory track record.
• Fermentation: ease of scaling up.
• Ease of Inclusion bodies purification.
23. DISADVANTAGES OF USING E.COLI
AS A SYSTEM
• Loss of plasmid and antibiotic property
• Unsolicited inducers for gene expression
• Intracellular accumulation of heterologous proteins as inclusion bodies
• Improper protein refolding
• Lack of post- translational modifications (including unable to form disulphide bonds)
• Protein-mediated metabolic burden and stress
• Endotoxin contamination
• Poor secretion
• Proteolytic digestion complexity in downstream processing
24. EXPRESSION VECTOR
• A circle of double-
stranded DNA that is
separate from the
chromosomes, which is
found in bacteria.
25. ADVANTAGES OF USING
BACTERIAL PLASMID
•Small, easy to handle
•Straightforward selection strategies
•Useful for cloning small DNA fragments
•(< 10kbp)
28. HOST CELL USED FOR INSULIN
PRODUCTION
•Saccharomyces
cerevisiae is an
eukaryotic microbe
system that is widely
used for protein
expression that require
post-translational
modification.
29. ADVANTAGES OF USING
SACCHAROMYCES CEREVISIAE
• Nonpathogenic
• Rapid growth (generation time ca. 80 min)
• Dispersed cells
• Ease of replica plating and mutant isolation
• Can be grown on defined media giving the investigator complete
control over environmental parameters
• Well-defined genetic system
• Highly versatile DNA transformation system
31. EXPRESSION VECTOR USED IN
SACCHAROMYCES CEREVISIAE
• Yeast artificial chromosome (YAC) is a
human-engineered DNA molecule used to
clone DNA sequences in bacterial and
yeast cells.
• It is a plasmid vector used to clone DNA
fragments larger than 100 kb and up to
3000 kb.
• Circular form when they are amplified or
manipulated in E. coli
• Rendered linear and of very large size when
introduced as cloning vectors in yeast
32. YAC CONSTITUENTS
It consists of several important regions
• TEL: the telomere which is located at each chromosome end, protects the
linear DNA from degradation by the nucleases.
• CEN: the centromere which is the attachment site for the mitotic spindle
fibers , “pulls” one copy of each duplicated chromosome into each new
daughter cell.
• ORI: the replication origin sequences which are specific DNA sequences that
allow DNA replication machinery to assemble on the DNA .
• - It is also know as ARS: autonomously replicating sequence vectors in yeast
33. YAC CONSTITUENTS
It also consists of specific sequences:
• Yeast selectable marker A and B: Selectable markers allow
easy isolation of yeast cells that have taken up the artificial
chromosome.
• Bacterial selectable marker: Such as ampicillin resistance ; for
screening and selection purposes.
• Recognition site: For restriction enzyme ligation such as EcoR1 ,
BamHi
34. ADVANTAGES OF YAC
• Extremely large DNA molecules (up to more than 1 Mb) can be
introduced and propagated in the form of yeast artificial chromosomes
(YACs) in S. cerevisiae.
• It can be used to express eukaryotic proteins that require
posttranslational modification.
• Screening and selection properties.
• YACs can be utilized to clone and assemble the entire genomes of an
organism.
• Physical mapping.
35. DISADVANTAGES OF YAC
• Low transformation efficiencies.
• YACs are very difficult to manipulate.
• Approximately 40 % of the YACs from most libraries
are deleted.
• Approximately 40-60% of the YACs from most
libraries are chimeric.
37. PHASES INVOLVED IN INSULIN
PRODUCTION
Upstream
Process
Downstream
Process
38. UPSTREAM PROCESS PHASE
Shaking bioreactor
Advantages:
Easy ,Visible ,Cheap, Depyrogenation feasible
Disadvantage:
Poor aeration
Impeller jams
Requires cleaning siliconizing & sterilization
lHigh space requirements in incubator
39. UPSTREAM PROCESS PHASE
Stir tank bioreactor
• Continuous bioreactor
processes continually feed
nutrients and medium into the
bioreactor while also
continually harvesting material
from the bioreactor.
40. UPSTREAM PROCESS PHASE
Stir tank bioreactor
• Agitator is introduced to disperse the
reactants thoroughly into the reaction
mixture immediately as they enter the
reactor.
• Product is continuously drawn out and
that’s why known for perfect mixing.
• Compositions at outlet and inside reactor
are the same.
41. ADVANTAGES OF STIR TANK BIOREACTOR
• Multi-gas and pH control
• Increased Capacity( 5 L to 500 L)
• Versatility
42. DISADVANTAGES OF STIR TANK
BIOREACTOR
• Costly
• High power due the presence of mechanical pumps
• Limitation of Weight Preparation
• Requires siliconizing
• Requires constant cleaning,
• Sterilization, depyrogenation
• Requires high Maintenance -Chiller, parts
44. DOWNSTREAM PROCESS
• Removal of insolubles is the first step and involves the capture
of the product as a solute in a particulate-free liquid. Typical
operations to achieve this are filtration, centrifugation.
• Product isolation is the removal of those components whose
properties vary considerably from that of the desired product.
Solvent extraction, adsorption, ultrafiltration, and precipitation are
some of the unit operations involved.
45. DOWNSTREAM PROCESS
• Product purification is done to separate those contaminants that
resemble the product very closely in physical and chemical
properties. operations include affinity, size exclusion, reversed
phase chromatography, ion-exchange chromatography,
crystallization and fractional precipitation.
• Product polishing describes the final processing steps which end
with packaging of the product in a form that is stable, easily
transportable and convenient.