production of recombinant insulin

1. Recombinant Insulin
By MOHIT KOHLI

2. Insulin Hormone: Invented by Banting and Best, 1921
Structure of Insulin:
Two chains of 30 and 21 amino acids, linked with disulphide bonds

3. Human Insulin is produced in Escherichia coli by inserting gene of insulin with suitable vector.
Product is known as Humulin, was produced by Eli Lily by Recombinant DNA Technology
Formation of Precursor pro-insulin

6. Insulin manufacture using recombinant DNA technology

7. Media Composition:
Growth media used in this method consists of:
• Magnesium sulphate heptahydrate
• Glycerol
• Biotin
• Yeast trace mineral (YTM) solution
• Potassium dihydrogen sulphate
• Calcium chloride dehydrate
• Ammonium sulphate
• Trace minerals (boric acid, sulphuric acid, manganese sulphate, ferric chloride
hexahydrate, zinc sulphate etc.)

8. Analysis of recombinant protein
IMAC purification