Hplc by deepankar 2018
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HPLC is a type of liquid chromatography that uses a pump to force a mobile phase through a column packed with solid particles or porous material at high pressure. This allows for high-speed and high-performance separation of mixtures into individual components. HPLC works on the principle of adsorption, where different components interact differently with the stationary phase in the column and therefore separate and elute at different rates when the mobile phase is passed through under pressure. The basic instrumentation of HPLC includes a pump, injector, column, detector, and computer.
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High performance liquid chromatography (hplc) by Muhammad Shakaib
High performance liquid chromatography (HPLC) is a technique used to separate components in a mixture. It works by differential migration of solutes through a column containing a stationary phase as a pressurized mobile phase flows through. Solutes are detected as they elute from the column. Key aspects of HPLC include the use of a high pressure pump to deliver the mobile phase, columns packed with microparticulate stationary phases, and detectors to identify eluted components. HPLC is useful for separating compounds that are not volatile enough for gas chromatography.
counter current chromatography
Counter current chromatography is a liquid chromatography technique that uses two immiscible liquid phases rather than a solid support. It works by partitioning a solute between the two liquid phases which flow in opposite directions, allowing separation. There are different types including droplet CCC which uses gravity, centrifugal partition chromatography which uses centrifugal force, and high speed CCC. Careful selection of solvent systems is important to achieve the desired partition coefficient for separation. CCC has applications in isolating compounds from natural products like plants.
High Performance Liquid Chromatography
High Performance Liquid Chromatography (HPLC) is a separation technique that involves injecting a small volume of liquid sample into a column packed with tiny particles. Individual components of the sample are then transported through the column by a mobile phase and separated based on interactions with the stationary phase. These separated components exit the column and are detected, providing a chromatogram. HPLC uses small particle sizes, high column pressures up to 6000-9000 psi, and flow rates of 1-3 mL/min to achieve fast, efficient, and high resolution separations of both volatile and non-volatile compounds.
Pumps
This document discusses different types of chromatography techniques and the pumps used in each. It covers high performance liquid chromatography (HPLC), ion-exchange chromatography, and size-exclusion chromatography. For HPLC, it describes reciprocating piston pumps that are able to deliver precise, pulse-free flow at high pressures up to 10,000 psi. For ion-exchange chromatography, it mentions pumps must provide pulse-free flow for sensitive detectors and single piston pumps are commonly used. Size-exclusion chromatography utilizes small volume reciprocating pumps for accurately controlled flow rates at pressures up to 7,250 psi.
H.P.L.C.
HPLC is a powerful analytical technique used to separate components in a mixture. It works by forcing a pressurized liquid mobile phase through a column containing a stationary phase. Compounds are separated based on how they interact with the stationary and mobile phases. HPLC is useful for analyzing pharmaceuticals, foods, biological samples, and more. It provides high resolution for separation, identification, and quantification of analytes. Common applications include drug analysis, food quality testing, and clinical diagnostics.
Countercurrent Chromatography
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Hplc high performance liquid chromatography
HPLC is a chromatographic technique used to separate components in a mixture. It works by pumping a pressurized mobile liquid phase through a column containing a stationary phase, which causes the components in a sample to separate as they are transported through the column at different rates. Key components of an HPLC system include solvent reservoirs, pumps to precisely deliver the mobile phase, an injector to introduce samples, columns for separation, detectors, and a data system to analyze results. HPLC offers advantages like high separation capacity, reproducibility, and ability to analyze a wide range of biological, medical, food, environmental, and industrial samples.
Hplc vs gc
HPLC and GC are both chromatography techniques used to separate mixtures. HPLC uses high pressure liquid mobile phases to move compounds through a densely packed solid stationary phase column, allowing for separation of fluid mixtures. GC uses an inert gas mobile phase to move vaporized or gaseous compounds through a liquid or polymer stationary phase, making it well-suited for analyzing purity. The major differences between the two techniques are that HPLC uses liquid mobile and solid stationary phases, while GC uses gas mobile and liquid stationary phases, and GC includes a temperature-controlled oven during the separation process.
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High performance liquid chromatography (hplc) by Muhammad Shakaib
High performance liquid chromatography (HPLC) is a technique used to separate components in a mixture. It works by differential migration of solutes through a column containing a stationary phase as a pressurized mobile phase flows through. Solutes are detected as they elute from the column. Key aspects of HPLC include the use of a high pressure pump to deliver the mobile phase, columns packed with microparticulate stationary phases, and detectors to identify eluted components. HPLC is useful for separating compounds that are not volatile enough for gas chromatography.
counter current chromatography
Counter current chromatography is a liquid chromatography technique that uses two immiscible liquid phases rather than a solid support. It works by partitioning a solute between the two liquid phases which flow in opposite directions, allowing separation. There are different types including droplet CCC which uses gravity, centrifugal partition chromatography which uses centrifugal force, and high speed CCC. Careful selection of solvent systems is important to achieve the desired partition coefficient for separation. CCC has applications in isolating compounds from natural products like plants.
High Performance Liquid Chromatography
High Performance Liquid Chromatography (HPLC) is a separation technique that involves injecting a small volume of liquid sample into a column packed with tiny particles. Individual components of the sample are then transported through the column by a mobile phase and separated based on interactions with the stationary phase. These separated components exit the column and are detected, providing a chromatogram. HPLC uses small particle sizes, high column pressures up to 6000-9000 psi, and flow rates of 1-3 mL/min to achieve fast, efficient, and high resolution separations of both volatile and non-volatile compounds.
Pumps
This document discusses different types of chromatography techniques and the pumps used in each. It covers high performance liquid chromatography (HPLC), ion-exchange chromatography, and size-exclusion chromatography. For HPLC, it describes reciprocating piston pumps that are able to deliver precise, pulse-free flow at high pressures up to 10,000 psi. For ion-exchange chromatography, it mentions pumps must provide pulse-free flow for sensitive detectors and single piston pumps are commonly used. Size-exclusion chromatography utilizes small volume reciprocating pumps for accurately controlled flow rates at pressures up to 7,250 psi.
H.P.L.C.
HPLC is a powerful analytical technique used to separate components in a mixture. It works by forcing a pressurized liquid mobile phase through a column containing a stationary phase. Compounds are separated based on how they interact with the stationary and mobile phases. HPLC is useful for analyzing pharmaceuticals, foods, biological samples, and more. It provides high resolution for separation, identification, and quantification of analytes. Common applications include drug analysis, food quality testing, and clinical diagnostics.
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Hplc high performance liquid chromatography
HPLC is a chromatographic technique used to separate components in a mixture. It works by pumping a pressurized mobile liquid phase through a column containing a stationary phase, which causes the components in a sample to separate as they are transported through the column at different rates. Key components of an HPLC system include solvent reservoirs, pumps to precisely deliver the mobile phase, an injector to introduce samples, columns for separation, detectors, and a data system to analyze results. HPLC offers advantages like high separation capacity, reproducibility, and ability to analyze a wide range of biological, medical, food, environmental, and industrial samples.
Hplc vs gc
HPLC and GC are both chromatography techniques used to separate mixtures. HPLC uses high pressure liquid mobile phases to move compounds through a densely packed solid stationary phase column, allowing for separation of fluid mixtures. GC uses an inert gas mobile phase to move vaporized or gaseous compounds through a liquid or polymer stationary phase, making it well-suited for analyzing purity. The major differences between the two techniques are that HPLC uses liquid mobile and solid stationary phases, while GC uses gas mobile and liquid stationary phases, and GC includes a temperature-controlled oven during the separation process.
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HPLC is a form of liquid chromatography that uses pumps to pass a pressurized mobile liquid phase through a column packed with solid particles. This allows the components of a dissolved sample to be separated as they are transported through the column at different rates depending on their interactions with the stationary and mobile phases. HPLC instruments consist of a pump, injector, column, and detector. Separation is based on the partitioning of compounds between the mobile and stationary phases, and detectors are used to measure separated components as they exit the column. HPLC provides efficient, sensitive, and high-pressure separations of sample mixtures.
Hplc in detail by Shree
HPLC PPT for you If You want to download it then download it .
Its is in original PPT form
Share it & improve your Knowledge.
INTRODUCTION
HPLC is a form of liquid chromatography used to separate compounds that are dissolved in solution.
HPLC instruments consist of a reservoir of mobile phase, a pump, an injector, a separation column, and a detector.
Compounds are separated by injecting a sample mixture into the column. The different component in the mixture pass through the column at differentiates due to differences in their partition behavior between the mobile phase and the stationary phase.
The mobile phase must be degassed to eliminate the formation of air bubbles.
Sample injection system in hplc
The document discusses sample injection systems used in high performance liquid chromatography (HPLC). It describes the key components of sample injection valves, including a needle or syringe, metering device, sample loop, and valve. It also summarizes different types of injection systems, including manual injection, pull-to-fill autosamplers, push-to-fill autosamplers, and integral-loop autosamplers. Each type follows similar steps of loading the sample, injecting it, and washing the system.
Instrumentation of HPLC, principle by kk sahu
INTRODUCTION
Instrumentation of HPLC
TYPES OF HPLC
PARAMETERS
APPLICATION
CONCLUSION
REFERENCE
High-performance liquid chromatography ( HPLC) is a specific form of column chromatography generally used in biochemistry and analysis to separate, identify, and quantify the active compounds.
HPLC mainly utilizes a column that holds packing material (stationary phase), a pump that moves the mobile phase(s) through the column, and a detector that shows the retention times of the molecules.
High performance liquid chromatography
HPLC is a form of liquid chromatography used to separate compounds that are dissolved in solution using a high pressure.
Counter current chromatography (unit- 7) priyansha singh notes
COUNTER CURRENT CHROMATOGRAPHY
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4. CHOICE OF SOLVENTS
5, MODE OF SEPARATION
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This document provides an overview of high performance liquid chromatography (HPLC). It describes the key components of an HPLC system, including the pump, injector, separation column, and detector. It explains how the different components of a mixture are separated through their interaction with the mobile and stationary phases in the column. The document also discusses various types of columns, detectors, and applications of HPLC in chemistry, biochemistry, and quality control.
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This document discusses high-performance liquid chromatography (HPLC), which is a widely used technique for separating and analyzing components in mixtures. It describes the basic components and principles of HPLC, including pumps to pass a pressurized liquid and sample mixture through a column containing stationary phase particles. The components interact differently with the stationary phase and are separated into bands that are then detected and analyzed. Common detectors described are UV-visible, fluorescence, and electrochemical detectors. The document also discusses various modes of operation like isocratic and gradient elution and types of columns and stationary phases used.
New microsoft office power point presentation
This document provides an overview of chromatography and high performance liquid chromatography (HPLC). It defines chromatography as a method to separate mixtures into individual components based on differences in how they move through a stationary and mobile phase. The document describes the basic principles, instrumentation, and applications of HPLC. Key components of HPLC systems discussed include pumps, injectors, columns, detectors, and various chromatography techniques like adsorption, partition, and ion exchange chromatography.
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Content include basic introduction to chromatography. Brief view of Liquid Chromatography. HPLC introduction, other names, types of HPLC, detailed instrumentation with image of each part, and applications. Sources of content described in 'References' entitled slide. This presentation was prepared for the partial fulfillment of Master of Pharmacy.
Supercritical fluid chromatography
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HPLC
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Hplc ppt
High Performance Liquid Chromatography (HPLC) is a separation technique that uses pumps to force a liquid mobile phase through a column packed with solid particles. Sample components interact differently with the stationary and mobile phases allowing separation. HPLC instruments consist of pumps, injectors, columns, detectors and computers. Samples are injected and the separated components are detected and data is analyzed to identify and quantify the components. HPLC is used in various fields to analyze complex mixtures like pharmaceuticals, chemicals, and biological samples.
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This document provides information about high performance liquid chromatography (HPLC). It begins by defining HPLC as a separation technique that uses a solid stationary phase and liquid mobile phase. It then discusses various modes of HPLC including normal phase, reverse phase, ion-exchange, and size-exclusion chromatography. The document also describes common HPLC instrumentation such as pumps, columns, detectors, and gives examples of their applications. It provides details on the classification, packing, and selection of HPLC columns.
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High performance liquid chromatography (HPLC) is an improved form of liquid chromatography that forces solvent through a column at high pressure. It separates mixtures by interacting differently with stationary and mobile phases in the column based on molecular structure. HPLC uses pumps to push solvent through an injector, column, detector, and recorder/computer. The column contains porous particles that substances differentially bind to. Detectors identify separated substances and recorders display chromatograms showing separation and quantification. HPLC has many applications like pharmaceutical quality control and forensic drug analysis due to its accuracy, precision, and versatility.
HPLC
HPLC is a type of liquid chromatography that is used to separate, identify, and quantify components in a mixture. It works by forcing a pressurized liquid mobile phase through a column packed with solid particles or porous material. Samples are injected into the column and the different components interact differently with the stationary phase, causing them to elute from the column at different rates and allowing separation. HPLC provides efficient, high resolution separations and is commonly used in fields like pharmaceutical analysis and quality control due to its ability to analyze complex mixtures.
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Chromatography HPLC Shabir
HPLC is a form of liquid chromatography that uses pumps to pass a pressurized mobile liquid phase through a column packed with solid particles. This allows the components of a dissolved sample to be separated as they are transported through the column at different rates depending on their interactions with the stationary and mobile phases. HPLC instruments consist of a pump, injector, column, and detector. Separation is based on the partitioning of compounds between the mobile and stationary phases, and detectors are used to measure separated components as they exit the column. HPLC provides efficient, sensitive, and high-pressure separations of sample mixtures.
Hplc in detail by Shree
HPLC PPT for you If You want to download it then download it .
Its is in original PPT form
Share it & improve your Knowledge.
INTRODUCTION
HPLC is a form of liquid chromatography used to separate compounds that are dissolved in solution.
HPLC instruments consist of a reservoir of mobile phase, a pump, an injector, a separation column, and a detector.
Compounds are separated by injecting a sample mixture into the column. The different component in the mixture pass through the column at differentiates due to differences in their partition behavior between the mobile phase and the stationary phase.
The mobile phase must be degassed to eliminate the formation of air bubbles.
Sample injection system in hplc
The document discusses sample injection systems used in high performance liquid chromatography (HPLC). It describes the key components of sample injection valves, including a needle or syringe, metering device, sample loop, and valve. It also summarizes different types of injection systems, including manual injection, pull-to-fill autosamplers, push-to-fill autosamplers, and integral-loop autosamplers. Each type follows similar steps of loading the sample, injecting it, and washing the system.
Instrumentation of HPLC, principle by kk sahu
INTRODUCTION
Instrumentation of HPLC
TYPES OF HPLC
PARAMETERS
APPLICATION
CONCLUSION
REFERENCE
High-performance liquid chromatography ( HPLC) is a specific form of column chromatography generally used in biochemistry and analysis to separate, identify, and quantify the active compounds.
HPLC mainly utilizes a column that holds packing material (stationary phase), a pump that moves the mobile phase(s) through the column, and a detector that shows the retention times of the molecules.
High performance liquid chromatography
HPLC is a form of liquid chromatography used to separate compounds that are dissolved in solution using a high pressure.
Counter current chromatography (unit- 7) priyansha singh notes
COUNTER CURRENT CHROMATOGRAPHY
1. BASIC PRINCIPLES
2. DROPLET CCC
3. CENTRIFUGAL PARTITION CCC
4. CHOICE OF SOLVENTS
5, MODE OF SEPARATION
6, APPLICATIONS
HPLC AND ITS PHARMACEUTICAL APPLICATIONS
THIS SLIDES CONTAINS A BRIEF INTRODUCTION ABOUT HPLC ,ITS INSTRUMENTATION AND APPLICATIONS MAINLY IN PHARMACEUTICAL AREAS
High Performance Liquid Chromatography HPLC
High Performance Liquid Chromatography HPLC is a process of separating components in a liquid mixture. A liquid sample is injected into a stream of solvent mobile phase flowing through a column packed with a separation medium stationary phase . Sample components separate from one another by a process of differential migration as they flow through the column.As bands emerge from the column, flow carries them to one or more detectors which deliver a voltage response as a function of time. This is called a chromatogram. For each peak, the time at which it emerges identifies the sample constituent with respect to a standard. The peak’s area represents the quantity .HPLC provides a highly specific, reasonably precise, and fairly rapid analytical method for a plethora of complicated samples.This is difficult in detecting compounds. Low sensitivity of some compounds towards the stationary phase in the columns is difficult. Mohd Ali | Panjak Chasta | Dr. Kausal Kishore Chandrul "High Performance Liquid Chromatography (HPLC)" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd45146.pdf Paper URL: https://www.ijtsrd.com/pharmacy/other/45146/high-performance-liquid-chromatography-hplc/mohd-ali
Hplc by naveed 27 nov 2018
This document provides an overview of high performance liquid chromatography (HPLC). It describes the key components of an HPLC system, including the pump, injector, separation column, and detector. It explains how the different components of a mixture are separated through their interaction with the mobile and stationary phases in the column. The document also discusses various types of columns, detectors, and applications of HPLC in chemistry, biochemistry, and quality control.
HPLC
This document discusses high-performance liquid chromatography (HPLC), which is a widely used technique for separating and analyzing components in mixtures. It describes the basic components and principles of HPLC, including pumps to pass a pressurized liquid and sample mixture through a column containing stationary phase particles. The components interact differently with the stationary phase and are separated into bands that are then detected and analyzed. Common detectors described are UV-visible, fluorescence, and electrochemical detectors. The document also discusses various modes of operation like isocratic and gradient elution and types of columns and stationary phases used.
New microsoft office power point presentation
This document provides an overview of chromatography and high performance liquid chromatography (HPLC). It defines chromatography as a method to separate mixtures into individual components based on differences in how they move through a stationary and mobile phase. The document describes the basic principles, instrumentation, and applications of HPLC. Key components of HPLC systems discussed include pumps, injectors, columns, detectors, and various chromatography techniques like adsorption, partition, and ion exchange chromatography.
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Content include basic introduction to chromatography. Brief view of Liquid Chromatography. HPLC introduction, other names, types of HPLC, detailed instrumentation with image of each part, and applications. Sources of content described in 'References' entitled slide. This presentation was prepared for the partial fulfillment of Master of Pharmacy.
Supercritical fluid chromatography
This document discusses supercritical fluid chromatography (SFC). It defines supercritical fluids and explains that SFC uses supercritical fluids like carbon dioxide as the mobile phase. This allows SFC to combine advantages of gas chromatography and liquid chromatography. The document outlines various topics in SFC including stationary phases, instrumentation, and applications in areas like separations and purification. It provides details on commonly used supercritical fluids, instrumentation components, and stationary phase materials like silica for SFC.
HPLC
CONTAINS ALL THE INSTRUMENTATIONS & NECESSARY DETAILS
CAN BE REFERRED BY PHARMACEUTICAL ANALYSIS STUDENTS
HPLC
This document provides an overview of high performance liquid chromatography (HPLC). It describes HPLC as a type of liquid chromatography that uses small particle sizes as the stationary phase to separate compounds dissolved in a solution. The key components of an HPLC system are a solvent delivery pump, injector, column, detectors, and data collection system. Different types of columns include normal phase, reverse phase, size exclusion, and ion exchange. Factors that affect HPLC separation include column length and temperature, flow rate, particle size, and mobile phase properties. HPLC is used for quantitative and qualitative analysis in various applications like analyzing drugs, pollutants, and food/drug products.
Hplc ppt
High Performance Liquid Chromatography (HPLC) is a separation technique that uses pumps to force a liquid mobile phase through a column packed with solid particles. Sample components interact differently with the stationary and mobile phases allowing separation. HPLC instruments consist of pumps, injectors, columns, detectors and computers. Samples are injected and the separated components are detected and data is analyzed to identify and quantify the components. HPLC is used in various fields to analyze complex mixtures like pharmaceuticals, chemicals, and biological samples.
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This document provides an overview of high performance liquid chromatography (HPLC). It discusses the principle of HPLC, which separates mixtures by forcing a mobile liquid phase through a column containing a stationary phase. The document describes the main components of an HPLC instrument, including the mobile phase reservoirs, pump, sample injection system, column, detector, and recorder. It provides details on each component, such as the types of pumps, columns, detectors commonly used. The document concludes by listing references used to compile the information presented.
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This document discusses high performance liquid chromatography (HPLC). HPLC uses a liquid mobile phase to separate components in a liquid or dissolved sample. The mobile phase passes through a column containing a stationary phase, which interacts with sample components to cause separation. Common stationary phases include bonded silica for reverse phase chromatography and ion exchange resins. Key aspects of HPLC covered include the instrument, mobile and stationary phases, sample introduction, detectors, and quantitative applications.
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This document provides information about high performance liquid chromatography (HPLC). It begins by defining HPLC as a separation technique that uses a solid stationary phase and liquid mobile phase. It then discusses various modes of HPLC including normal phase, reverse phase, ion-exchange, and size-exclusion chromatography. The document also describes common HPLC instrumentation such as pumps, columns, detectors, and gives examples of their applications. It provides details on the classification, packing, and selection of HPLC columns.
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High performance liquid chromatography (HPLC) is an improved form of liquid chromatography that forces solvent through a column at high pressure. It separates mixtures by interacting differently with stationary and mobile phases in the column based on molecular structure. HPLC uses pumps to push solvent through an injector, column, detector, and recorder/computer. The column contains porous particles that substances differentially bind to. Detectors identify separated substances and recorders display chromatograms showing separation and quantification. HPLC has many applications like pharmaceutical quality control and forensic drug analysis due to its accuracy, precision, and versatility.
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HPLC involves forcing a pressurized mobile liquid phase through a column packed with solid particles that separates the components of a injected sample mixture. Key aspects of HPLC include the pump that drives the mobile phase, the injector that introduces the sample, the column that performs the separation, and a detector that provides a signal output used to generate a chromatogram. HPLC can be used for both qualitative and quantitative analysis in various fields such as pharmaceuticals, biochemistry, and environmental analysis.
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This document provides an overview of high performance liquid chromatography (HPLC). It begins by defining chromatography and its basic components. It then discusses the development of HPLC and how it works, including the instrumentation used. The document outlines different types of HPLC based on the mode of chromatography, principle of separation, elution technique, and type of analysis. It provides examples of each type. Finally, the document lists several applications of HPLC in fields like chemistry, biochemistry, quality control, and pharmacokinetics.
High pressure liquid chromatography, B.Pharm
HPLC is a form of liquid chromatography that can separate compounds dissolved in solution. It works by injecting a sample into a column packed with tiny particles, then using a pump to force a liquid mobile phase through the column. This carries the sample components along the column at different speeds based on their interaction with the stationary phase, separating them. HPLC can separate a wide range of compounds and is used in pharmaceutical and chemical analysis applications.
HPLC
HPLC- introduction, principle, types, working, instrumentation and operations of HPLC has been included with appropriate gifs and images for better understanding. What are all the things need to be known by a science student about HPLC (basics and working) is clearly given in this presentation.
Hplc presentation
The document discusses High Performance Liquid Chromatography (HPLC), including its principles, types of separations, instrumentation, applications in fields like food and pharmaceuticals, and advantages like speed and accuracy over traditional chromatography techniques. HPLC uses high pressure to pass samples in liquid mobile phases through stationary phases in columns to separate mixtures based on properties like polarity, size, or ionic charge.
HPLC.pptx
Chromatography separates mixtures into components based on molecular structure and composition. High performance liquid chromatography (HPLC) is a highly improved form of liquid chromatography that forces solvents through columns under high pressure, making it faster. HPLC instruments include pumps to force mobile phases, injectors for samples, columns for separation, detectors to analyze eluents, and data collection systems. HPLC is used in pharmaceutical quality control, environmental monitoring, forensics, food and flavor analysis, and clinical testing.
About HPLC!
An introduction to HPLC(High Performance LIquid Chromatography) was depicted in the presentation.
Simultaneously, the each and every component of HPLC was explained by depicting with a diagram in the slide.
The key notes are also included in the presentation.
hPLC NEW ONE.pptx
This document provides an overview of fundamentals of high performance liquid chromatography (HPLC). It explains that HPLC involves injecting a liquid sample into a column packed with tiny particles and using a liquid mobile phase to separate the sample components. The major components of an HPLC system are identified as the pump, injector, column, detector and computer. Common applications of HPLC include qualitative analysis to identify compounds, quantitative analysis to measure amounts, and trace analysis to detect very low concentration compounds.
Chromatography( HPLC, ion exchange chromatography, Gas chromatography)
it consists of notes for hplc , ion exchange chromatography, gas chromatography principle , instrumentation, applications
HPLC .pdf
HPLC is a type of column chromatography that uses high pressure to force a mobile liquid phase through a column packed with solid particles. This allows for faster separation of mixtures compared to standard column chromatography. HPLC instruments consist of a pump that forces the mobile phase through the column at high pressure, an injector for samples, a column for separation, a detector, and a computer system. There are different types of HPLC columns that separate mixtures based on polarity, ionic interactions, size, or other properties. HPLC is used in various applications to separate, identify, and quantify components in mixtures.
Similar to Hplc by deepankar 2018 (20)
HPLC.pptx principle, instrumentation , theory, columns
HPLC.pptx principle, instrumentation , theory, columns
Chromatography( HPLC, ion exchange chromatography, Gas chromatography)
Chromatography( HPLC, ion exchange chromatography, Gas chromatography)
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原版制作(carleton毕业证书)卡尔顿大学毕业证硕士文凭原版一模一样
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学校原件一模一样【微信：741003700 】《(salfor毕业证书)索尔福德大学毕业证》【微信：741003700 】学位证，留信认证（真实可查，永久存档）原件一模一样纸张工艺/offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原。
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among stars.
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s
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Hplc by deepankar 2018
- 1. HPLCHIGH PERFORMANCE LIQUID CHROMATOGRAPHY SUBMITTED BY: DEEPANKAR DIVYA NAIR KAJAL MSC.H- 1ST YEAR SUBMITTED TO: DR.PRAVEEN RISHI
- 2. INTRODUCTION • HPLC stands for “High-performance liquid chromatography”(sometimes referred to as High- pressure liquid chromatography). • High performance liquid chromatography is a powerful tool in analysis, it yields high performance and high speed compared to traditional columns chromatography because of the forcibly pumped mobile phase.
- 3. • HPLC is a chromatographic technique that can separate a mixture of compounds. • It is used in biochemistry and analytical chemistry to identify, quantify and purify the individual components of a mixture.
- 4. • CHROMATOGRAPHY : physical method in which separation of components takes place between two phases-a stationary phase and a mobile phase. • STATIONARY PHASE: The substance on which adsorption of the analyte (the substance to be separated during chromatography) takes place. It can be a solid ,gel, or a solid liquid combination. • MOBILE PHASE: solvent which carries the analyte (a liquid or a gas).
- 5. • HPLC is a type of liquid chromatography where the sample is forced through a column that is packed with a stationary phase composed of irregularly or spherically shaped particles,a porous monolithic layer, or a porous membrane by a liquid (mobile phase) at high pressure.
- 6. PRINCIPLE • To understand the principle of HPLC,we must first look at the principle behind liquid chromatography. • Liquid chromatography is a separation technique that involves :-the placement (injection) of a small volume of liquid sample into a tube packed with porous particles(stationary phase) where individual components of the sample are transported along the packed tube by a liquid moved by gravity. • The main principle of separation is adsorption.
- 7. • HPLC is a separation technique that involves: • The injection of a small volume of liquid sample into a tube packed with tiny particles(3-5 micron) in diameter called stationary phase. • Where individual components of the sample are moved down the packed tube (column) with a liquid (mobile phase) forced through the column by high pressure delivered by a pump.