fluid chromatography (SFC) can be used on an analytical
scale.
It is a combination of High performance liquid chromatography (HPLC)
and Gas chromatography (GC).
It can be used with non-volatile and thermally labile analytes.
It can be used with the universal flame ionization detector.
It is important to producing narrower peaks due to rapid diffusion.
It is important for the chiral separations and analysis of high-molecularweight
hydrocarbons.
Supercritical fluids are suitable as a substitute for organic solvents in a
range of industrial and laboratory processes.
Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase.
Validation of Analytical and Bioanalytical methodssarikakkadam
Guidelines for Validation of Analytical and Bioanalytical methods as per ICH (Q2R1) and USFDA respectively with an example of Bioanalytical method validation.
fluid chromatography (SFC) can be used on an analytical
scale.
It is a combination of High performance liquid chromatography (HPLC)
and Gas chromatography (GC).
It can be used with non-volatile and thermally labile analytes.
It can be used with the universal flame ionization detector.
It is important to producing narrower peaks due to rapid diffusion.
It is important for the chiral separations and analysis of high-molecularweight
hydrocarbons.
Supercritical fluids are suitable as a substitute for organic solvents in a
range of industrial and laboratory processes.
Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase.
Validation of Analytical and Bioanalytical methodssarikakkadam
Guidelines for Validation of Analytical and Bioanalytical methods as per ICH (Q2R1) and USFDA respectively with an example of Bioanalytical method validation.
Gas chromatography and its instrumentationArgha Sen
Gas chromatography is an unique technology which helps us in separating volatile analytes. Its is an easy and reproduciple method for detecting residual solvents found in APIs.
Gas chromatography, an introduction.pdfSherif Taha
This lecture presents an introduction to the beginner user on the usage of the gas chromatography technique. The main topics are; selecting the injection technique, suitable liner, column of separation, and developing an efficient temperature program.
HPLC is a High Performance liquid Chromatography.
High Pressure Liquid Chromatography.
High Priced Liquid Chromatography.
It is column chromatography.
It is Liquid Chromatography.
It is modified from of gas chromatography, it is applicable for both Volatile as well as Non volatile compound.
It can mainly divided by two types 1. Normal phase HPLC 2. Reversed Phase HPLC.
It is having a high resolution and separation capacity.
This Powerpoint presentation helps us to know the basic working principles, instrumentation an advantage of super critical fluid chromatography.
Contact Details:
Anbu Dinesh Jayakumar
M.Pharmacy ( Pharmaceutical Chemistry)
Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore
Mobile : 8838404664 / 8608890121( Whatsapp)
Email: anbudinesh007@gmail.com
HPLC[ HIGH PERPROMANCE LIQUID CHROMATOGRAPHY OR HIGH PRESSURE LIQUID CHROMAT...Dr. Ravi Sankar
GASSCHROMATOGRAPHY[GC], ADVANCED STUDY OF THE FOLLOWING AND THEIR APPLICATIONS, INTRODUCTION, THEORY, COLUMN OPERATION,INSTRUMENTATION AND DETECTION,APPLICATIONS AND ADVANTAGES OF GC,PRINCIPLE OF SEPARATION IN GC, HOW GC MECHINE WORKS? COLUMN, DETECTORS.
BY P.RAVISANKAR, VIGNAN PHARMACY COLLEGE, VADLAMUDI, GUNTUR, A.P, INDIA.
Gas chromatography and its instrumentationArgha Sen
Gas chromatography is an unique technology which helps us in separating volatile analytes. Its is an easy and reproduciple method for detecting residual solvents found in APIs.
Gas chromatography, an introduction.pdfSherif Taha
This lecture presents an introduction to the beginner user on the usage of the gas chromatography technique. The main topics are; selecting the injection technique, suitable liner, column of separation, and developing an efficient temperature program.
HPLC is a High Performance liquid Chromatography.
High Pressure Liquid Chromatography.
High Priced Liquid Chromatography.
It is column chromatography.
It is Liquid Chromatography.
It is modified from of gas chromatography, it is applicable for both Volatile as well as Non volatile compound.
It can mainly divided by two types 1. Normal phase HPLC 2. Reversed Phase HPLC.
It is having a high resolution and separation capacity.
This Powerpoint presentation helps us to know the basic working principles, instrumentation an advantage of super critical fluid chromatography.
Contact Details:
Anbu Dinesh Jayakumar
M.Pharmacy ( Pharmaceutical Chemistry)
Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore
Mobile : 8838404664 / 8608890121( Whatsapp)
Email: anbudinesh007@gmail.com
HPLC[ HIGH PERPROMANCE LIQUID CHROMATOGRAPHY OR HIGH PRESSURE LIQUID CHROMAT...Dr. Ravi Sankar
GASSCHROMATOGRAPHY[GC], ADVANCED STUDY OF THE FOLLOWING AND THEIR APPLICATIONS, INTRODUCTION, THEORY, COLUMN OPERATION,INSTRUMENTATION AND DETECTION,APPLICATIONS AND ADVANTAGES OF GC,PRINCIPLE OF SEPARATION IN GC, HOW GC MECHINE WORKS? COLUMN, DETECTORS.
BY P.RAVISANKAR, VIGNAN PHARMACY COLLEGE, VADLAMUDI, GUNTUR, A.P, INDIA.
Email Marketing: Seven tips for writing (really) effective subject linesClose.io
According to MailChimp, the average open rate for marketing emails is 22%. At Close.io, we know that a good email marketing campaign has open rates up to 50%! If you want numbers like that, then you need to learn to write exceptional subject lines. Here's how.
With all the optimization work that goes into PPC, it’s easy to lose sight of the actual ad copy. Even though it is the only thing the searcher sees, ad copy often stagnates as the ad testing plan winds down and the enthusiasm brought about by a new project subsides into the steady hum of routine tasks.
In this webinar presentation, experts from Hanapin Marketing and Brainlabs Digital go through 10 unique ways to breathe new life back into your PPC ads and discuss why testing that ad copy is such an important tactic in your strategy.
Here at Table19, we believe that great work is only possible when clients and their agencies work together as a team. This is a presentation written by our Executive Creative Director Graham Wall, who on his first day in this industry heard the senior team he was shadowing say something he couldn’t understand: that the client had bought the wrong idea.
This set in motion a desire to understand how and why this had happened, and make sure it never happened again. This presentation details Graham’s learnings and philosophies, and shows how agencies and clients can create better work together.
Design, Promote, Repeat: How Long-Term Marketing Strategies Lead to SuccessShortStack
Brand awareness is key. One goal of any business should be to be the first one that comes to mind when people are in need of a particular product or service. Brand recognition has a direct impact on the success of all businesses. Researchers refer to this concept as “brand equity,” which gauges how consumers react to a brand’s name1. Brand equity and awareness aren’t automatic; companies must employ frequent attempts to introduce their brand and services to their target audience.
We held the largest ever Virtual SlideShare Summit a week back, if you missed it here's your chance to hear from the experts once more on some of the takeaways on presentation design and SlideShare Marketing
The technologies and people we are designing experiences for are constantly changing, in most cases they are changing at a rate that is difficult keep up with. When we think about how our teams are structured and the design processes we use in light of this challenge, a new design problem (or problem space) emerges, one that requires us to focus inward. How do we structure our teams and processes to be resilient? What would happen if we looked at our teams and design process as IA’s, Designers, Researchers? What strategies would we put in place to help them be successful? This talk will look at challenges we face leading, supporting, or simply being a part of design teams creating experiences for user groups with changing technological needs.
How to Become a Thought Leader in Your NicheLeslie Samuel
Are bloggers thought leaders? Here are some tips on how you can become one. Provide great value, put awesome content out there on a regular basis, and help others.
Chromatography is a widely used analytical technique in chemistry and biochemistry. It is a method for separating and identifying the individual components of a mixture based on their physical and chemical properties. The word "chromatography" itself means "color writing," and it was initially developed for separating and analyzing colored compounds, but it is now applied to a broad range of substances.
Chromatography is a powerful tool in research, quality control, and various industries because it allows for the separation and identification of complex mixtures, helping scientists and analysts understand the composition of substances and solve various analytical problems.
In gas chromatography, the sample is first converted to the vapor state or volatile compound is
used which are injected into heated port along with a carrier gas. Analyte in the vapor state
distributes between the stationary phase and the carrier gas. As the analyte elute from column
signal is displayed in the form of chromatogram. Measurements of GC are rapid and convenient.
Retention times are used for qualitative identification. Peak areas are used for quantitative
measurements.
1. HPLC vs GC
HPLC and GC are both methods of separation of compounds from a mixture. Whereas HPLC applies to
constituents that are fluids, GC is used when the compounds are gaseous or can be vaporized during
separation process. Both have the same underlying principle of heavy molecules flowing slower than lighter
ones. While HPLC refers to High Pressure Liquid Chromatography, GC is simply Gas Chromatography.
This article will highlight the differences between these two separation techniques.
HPLC
The technique of HPLC is made heavy use of in analytical chemistry to identify and analyze the individual
components of a mixture. In HPLC, columns and high pressure are used. High pressure ensures movement
of constituents in a mobile phase. It also moves the analyte in a densely packed column. Smaller particle
size helps in increasing the density of the constituents that helps in better separation when columns of
shorter length are used.
GC
Gas Chromatography on the other hand is mainly used to check the purity of a substance, and in some
cases, also helps in identifying a substance. There are two phases, the mobile, and the stationary phase in
the process. In the mobile phase, the carrier is an inert gas such as helium. Sometimes, nitrogen is used
as it is unreactive. The stationary phase involves using a polymer or a layer of liquid on an inert solid base.
What is the difference between HPLC and GC?
The major difference between GC and HPLC lies in the phases used. While in GC, compounds of a mixture
are separated using a liquid (stationary) phase and a gas (mobile) phase, in case of HPLC the stationary
phase is a solid while liquids make up mobile phase. Another difference lies in temperature control during
the process. In GC, there is an oven to contain the column consisting of gas phase and it can control the
temperatures when the gases are passing through the column. On the other hand, there is no such provision
of temperature control in HPLC. The last difference pertains to the concentration of the compounds. In GC,
it is the vapor pressure of the gases that decide the concentration of compounds whereas it is possible to
increase or decrease the concentration of the compounds in HPLC.
