This document provides an overview of high performance thin layer chromatography (HPTLC). It begins by defining HPTLC and comparing it to traditional thin layer chromatography (TLC). Key differences noted are that HPTLC uses smaller particle size coatings, allowing for higher efficiency separations over shorter distances. The document then outlines the basic steps for HPTLC including selecting the chromatographic layer, sample preparation, pre-washing and conditioning the layer, applying the sample, developing the plate, detecting spots, scanning and documentation. Various detection techniques and applications of HPTLC are also discussed.