This document discusses grafting and budding techniques in horticulture. It defines grafting as joining parts of two plants together so they unite and function as one plant. The key parts of a graft are the scion, which provides the shoot system, and the rootstock, which forms the root system. Successful grafting requires bringing the cambium layers of the scion and rootstock together. Several grafting methods are described, including whip grafting, cleft grafting, and approach grafting. The formation of the graft union and callus tissue bridging the scion and rootstock are also explained.
Use of growth regulators in seed production of Vegetable SimranJagirdar
WHAT ARE GROWTH REGULATORS?
A growth regulator is
An organic compound,
Can be natural or synthetic,
It modifies or controls one or more specific physiological processes within a plant but the sites of action and production are different.
If the compound is produced within the plant, it is called a plant hormone.
Both internal plant hormones and lab-created hormones are called plant growth regulators
The ‘Plant Hormones’ are natural and ‘Plant Growth Regulators’ are synthetic in nature.
The inability or Failure of two different plant Grafted together to produce a successful graft union is called Graft incompatibility.
Some pear cultivars are successfully grafted on quince rootstock, whereas, the other may die soon. However the reverse combination i.e. the quince on pear rootstock is always a failure
Use of growth regulators in seed production of Vegetable SimranJagirdar
WHAT ARE GROWTH REGULATORS?
A growth regulator is
An organic compound,
Can be natural or synthetic,
It modifies or controls one or more specific physiological processes within a plant but the sites of action and production are different.
If the compound is produced within the plant, it is called a plant hormone.
Both internal plant hormones and lab-created hormones are called plant growth regulators
The ‘Plant Hormones’ are natural and ‘Plant Growth Regulators’ are synthetic in nature.
The inability or Failure of two different plant Grafted together to produce a successful graft union is called Graft incompatibility.
Some pear cultivars are successfully grafted on quince rootstock, whereas, the other may die soon. However the reverse combination i.e. the quince on pear rootstock is always a failure
Postharvesting handling of flowers
Post harvesting handling of flowers
Flower production
Horticulture
Floriculture
Post harvesting of ornamental crops
Value addtion to flowers
Value addition to ornamental crops
Value addition in floriculture
Post harvesting handeling of cut and loose flowers
Cut flower
Loose flower hanfling
Postharvesting handling of flowers
Post harvesting handling of flowers
Flower production
Horticulture
Floriculture
Post harvesting of ornamental crops
Value addtion to flowers
Value addition to ornamental crops
Value addition in floriculture
Post harvesting handeling of cut and loose flowers
Cut flower
Loose flower hanfling
To improve the knowledge about principle and practice of canopy management in...AmanDohre
To improve the knowledge about principle and practice of canopy management in fruit crop
Enhancing understanding of canopy management principles and practices in fruit crops is paramount for optimizing orchard productivity. This involves comprehending canopy architecture, growth patterns, and physiological processes influencing fruit production. Through targeted practices such as pruning, thinning, and training, canopy structure, light penetration, and airflow are optimized, promoting balanced vegetative growth, flowering, and fruit set. Moreover, knowledge of rootstock-scion interactions allows for tailored canopy management strategies, further enhancing fruit quality and yield. By honing canopy management expertise, growers can maximize resource utilization, mitigate disease susceptibility, and improve overall fruit crop sustainability and profitability.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Unveiling the Energy Potential of Marshmallow Deposits.pdf
Grafting & budding
1. Practical 4
To study propagation methods through grafting and budding.
PRODUCTION TECHNOLOGY OF FRUIT AND PLANTATION CROPS
HRT341
2. Easy to Root type: Cutting & Layering
Hard to root type: Grafting & budding
Asexual Reproduction in Plants
3. ⦿Vegetative propagation in which shoot of a
superior plant is attached to root stock of
an inferior plant to multiply the superior
plant.
⦿The small piece of shoot which contribute
the upper part of the graft is called scion.
⦿ The plant that offers the lower part of
the graft is known as root stock.
4. GRAFTING
• Joining parts of two plants together in such a
manner that they unite and function as one
plant
•A graft has two parts, Scion and Stock.
SCION
• Upper part of graft combination which is
taken from the desired plant having superior
qualities - becomes the shoot system of the
graft.
5. GRAFTING…
STOCK (Root stock, Under stock )
• Part of the graft that forms the root system of
the grafted plant.
• In most cases, stock is raised from seeds
• The plant selected as a stock should be healthy
and vigorously growing
• Should be compatible with Scion
• Age , preferably 1 year
• Should be locally adapted, highly resistant and
with good efficiency for absorption of water and
minerals.
7. FORMATION OF GRAFT UNION
1. Adhesion of the root stock and scion
• Stock and Scion should be held together
firmly by wrapping, tying etc. so that the
parts will not move about.
• Success of grafting involves bringing the
cambium of the stock and scion together
and no graft union takes place unless it is
achieved.
8. FORMATION OF GRAFT UNION…
2. Proliferation of callus at the graft interface Formation of
callus (Parenchyma cells) by the cambium of stock and scion
– proliferate in 1
• -7 days.
3.Intermingling and interlocking of parenchyma cells of callus
of both graft components
• Fills the space between scion and stock
9. FORMATION OF GRAFT UNION…
4. Formation of vascular cambium
Differentiation of certain parenchyma cells to
form the vascular cambium.
5. Formation of new vascular tissues by the new
cambium – making contact between the
vascular tissues of the stock and scion –
nutrients
and metabolites between
permits translocation of water,
the stock and
scion.
11. Season: Veneer grafting should be performed in the month of September to
October
After Care
● Grafted plants / seedlings are kept humid and moist condition.
● Scion shoot starts sprouting in about 3 to 4 weeks.
● Polythene strip should be removed after the success of graft.
● Grafts ready for planting in 3 months. Success rate is 75 to 80 %.
Veneer Grafting
12. Stone Grafting
Selection of Scion: Scion sticks with 7 to 8 cm long from
current year growth from healthy mother plants should be
selected.
Procedure
●Stone grafting operation should be performed in July- August
months.
●Vertical cut of 3 to 4 cm is given on the rootstock and a
corresponding wedge shaped cut is given on the scion.
●Wedge shaped cut on scion is matched with the cut on rootstock
and then tied firmly with a polythene strip.
13. Stone Grafting
After Care
● Remove the growing shoots from root stock and inflorescence from
grafted scion immediately after emergence.
● Remove the polythene strip when union is formed and protect the graft
from hot sun, pest and disease attack.
Advantages
● Survival Success is more than 80 to 90 %, Requires less time and this
Method is very suitable for coastal region.
14.
15. 5. Grafted Scion 6. Successful Grafts
1. Selection of bud sticks 2. wedge shaped Cut on
Scion
3. Vertical Cut on Rootstock
4. Grafting and Polystriping
16. Inarch Grafting
Selection of Scion and Rootstock:
● Select one year old at least two feet long and healthy rootstock
grown in pots / polythene bags.
● Root stock plant and scion stock plant sticks should have equal
thickness. It should be from current year growth and from
healthy mother plant
Season
● August-September is best season for Inarch Grafting.
17. Procedure:
●Arrange the root stocks and scion tree on some platform or
mandapam and Mark the grafting locations on stock and scion.
●Remove 5 cm long, 1 to 2 cm wide & about 0.2 cm deep slice
of bark along with wooden part from stock and scion branches.
●Bring the cut surfaces together, cover the joint with a banana leaf
sheath and tie them together with soft threads and cover joint part
with cow dung plaster to protect from rain water.
Inarch Grafting
18. After care
●Water the plants as and when required. Cut the scion from the
parent tree after 2 to 3 months when the wound has healed.
●One week after separating the plant from root stock, the part of
the rootstock above the graft is cut off.
●Keep the graft in semi shading area to harden the graft before
transplanting into the main field.
Inarch Grafting
34. Saddle grafting
• useful for machine grafting, bench grafting of grape and Rhododendron
• scion and stock should be the same size grafting is done when stock and
scion are
• dormant, then the completed graft is stored in a grafting case until the graft un
has healed
37. Cleft grafting
• useful for topworking fruit trees, crown-grafting gra
• the best time is early spring, before active growth
• wedge grafting allows 1 more scion per stock
38.
39.
40. Bark grafting
Two types (rind and inlay-bark grafts)
differ only in prep of stock’s bark, which
should be slipping
often used in lieu of cleft graft later in the
season
43. Side grafting
defn: (smaller) scion inserted into the side
of a (larger) stock
Types
■side-stub: nursery trees too large for whip-and-
tongue, not large enough for cleft
■side-tongue: useful for broad- and narrow-
leaved evergreens (e.g., oriental arbovitae)
■side-veneer: useful for small potted plants, e.g.,
upright junipers
44.
45.
46. Approach grafting
two independent plants are grafted
together
after union, the top of the stock and
the base of the scion are removed
used when other methods are
unsuccessful (e.g., Camellia)
often done on plants in containers
three methods: spliced-, tongued-,
and inlay-approach grafting
49. Inarching
used for repairing damaged roots of
a full-grown tree.
seedlings are planted around the
tree during the dormant season,
grafting is done in the spring.
50.
51. Bridge grafting
used for repairing a damaged
trunk
early spring (with the bark
slipping) is the best time
(dormant) scion wood should be
1/4 to 1/2 in. diam.
52.
53.
54. Technique
Bark grafting
Date
Mid-April through mid-May
Use
Establish a pollinating variety on a limb of a tree or
to completely topwork a tree.
Bridge grafting Mid-April through mid-May Repair trees girdled above the ground line.
Cleft grafting Late February and March Establish a pollinating variety on a limb of a tree or
to completely topwork a tree. Limbs should be 1
inch or more in diameter.
Inarch grafting Mid-April through mid-May Repair trees girdled at or below the ground line.
Also used if a root disease is suspected or feared.
Saw-kerf grafting February and March On peaches, nectarines and plums to completely
topwork a tree.
Whip grafting February and early March Propagate 1-year-old rootstocks. May also be used
to establish a pollinating limb on a young,
established tree.
55. The Biology of Grafting
● Natural grafting
◦ Bracing of limbs in commercial orchards to
support weight of fruit
◦ Root grafting in woods is prevalent (CHO’s of
upper canopy trees provide support for
understory trees). This grafts only occur
between trees of the same species
◦ Problems with root grafting include:
transmission of fungi, bacteria and viruses
between plants (Dutch Elm Disease spreads this
way)
56.
57. The Biology of Grafting
● Formation of the graft union
◦ A “de novo” formed meristematic area
must develop between scion and rootstock
for a successful graft union
● 3 events
◦ 1) adhesion of the rootstock & scion
◦ 2) proliferation of callus at the graft
interface = callus bridge
◦ 3) vascular differentiation across the graft
interface
58.
59. The Biology of Grafting
● Steps in graft union formation
◦ 1.) lining up of the vascular cambium of rootstock and
scion. Held together with wrap, tape, staples, nails or
wedged together
◦ 2.) wound response
Necrotic layer 1 cell deep forms on both scion and stock
Undifferentiated callus tissue is produced from uninjured
parenchyma cells below the necrotic layer
Callus forms a wound periderm (outer “bark”) which becomes
suberized to prevent entry of pathogens
Necrotic layer dissolves
60. The Biology of Grafting
◦ 3.) callus bridge formation
Callus proliferates for 1 - 7 days
Callus mostly comes from scion (due to
basal movement of auxins and CHO’s, etc.)
An exception to this is on established
rootstock which can develop more callus
than that from the scion.
Adhesion of scion and stock cells with a mix
of pectins, CHO’s and proteins. Probably
secreted by dictyosomes which are part of
the Golgi bodies in cells.
61.
62. The Biology of Grafting
◦ 4.) Wound-repair :
First the xylem and then the phloem is
repaired
Occurs through differentiation of vascular
cambium across the callus bridge
Process takes 2 - 3 weeks in woody plants
◦ 5.) Production of 2º xylem and phloem
from new vascular cambium in the callus
bridge
Important that this stage be completed before
much new leaf development on scion or else
the leaves will wilt and the scion may die
63. The Biology of Grafting
Some water can be translocated through
callus cells but not enough to support leaves
Cell-to-cell transport via plasmodesmata =
symplastic transport (links cells membranes)
Apoplastic transport is between adhering
cells
64.
65. Graft Incompatibility
●Compatibility = ability of two
different plants grafted together to
produce a successful union and
continue to develop satisfactorily
●Graft failure: caused by anatomical
mismatching/poor craftmanship,
adverse environment, disease and
graft incompatibility
66. Graft Incompatibility
● Graft incompatibility from:
◦ Adverse physiological responses
between grafting partners
◦ Virus transmission
◦ Anatomical abnormalities of the
vascular tissue in the callus bridge
67. Graft Incompatibility
● External symptoms of incompatibility
◦ Failure of successful graft or bud union in
high percentages
◦ Early yellowing or defoliation in fall
◦ Shoot die-back and ill-health
◦ Premature death
◦ Marked differences in growth rate of scion
and stock
Overgrowth at, above or below the graft union
Suckering of rootstock
Breakage at the graft union
68. Graft Incompatibility
●Anatomical flaws leading to
incompatibility
◦ Poor vascular differentiation
◦ Phloem compression and vascular
discontinuity
◦ Delayed incompatibility may take 20 years
to show up (often in conifers and oaks)
69. Graft Incompatibility
● Physiological and Pathogen-Induced
Incompatibility
◦ Non-translocatable = localized. Problem is fixed
by using mutually compatible interstock(no
direct contact between scion and stock)
◦ Translocatable = spreads. Interstock does not
solve the problem. Some mobile chemical
causes phloem degradation. Ex: cyanogenic
glucosides like prunasin is converted to
hydrocyanic acid (from Quince to pear)
70. Graft Incompatibility
◦ Pathogen-induced virus of phytoplasma
induced
◦ Tristeza = viral disease of budded sweet
orange that is grafted onto infected
sour orange rootstock
76. WHIP (SPLICE) GRAFTING…
• Stock and scion of the same thickness are
selected.
• A slanting cut of about 3 – 5cm long is made
on the stock and a similar cut is made on the
scion.
• These two cut surfaces are placed together
and tightly tied with polyethylene grafting
type , which is removed when the graft
union is complete. (Apple, Pear, Cherry )
78. WHIP AND TONGUE GRAFTING…
• The stock and scion should be of equal
diameter
• A slanting cut of about 3 -5 cm long is made
at the top of the root stock and a similar cut
is made at the bottom of the scion.
• On each of these cut surfaces , a reverse cut
is made beginning at a point about 1/3 of the
distance from the tip and should be about
1/2 the length of the first cut.
79. WHIP AND TONGUE GRAFTING…
• The scion is then slipped into the stock so
that the tongues interlock and the cambium
of the stock and scion are in close contact.
These portions are then tied and wrapped
with grafting tape.
81. CLEFT GRAFTING (SPLIT GRAFTING)…
• Useful for grafting older plants with thick
stem
• The stock is cut at an appropriate height
• A vertical split for a distance of 7 – 9 cm
down the centre of the stock is made.
• This vertical split is kept open with the help
of a screw driver/ chisel etc.
• The scion should be made from dormant, 1
year old wood.
82. CLEFT GRAFTING (SPLIT GRAFTING)…
• Scions, 8 to 10 cm long, having 2 -3 buds are
selected.
• Basal end of each scion should be cut into a
sloping wedge (about 5 cm long).
• Scions are inserted in the sides of the vertical
split so that the cambium layer of the stock
matches with the scion and secured tightly with
waxed cloth.
84. WEDGE GRAFTING…
• Done in late winter or early spring before the
bark begins to slip.
• A 5 cm long “V” shaped wedge is cut on the
side of the stock (5-10 cm) – 2 or 3 such cuts can
be made depending on the diameter of the
stock – the cut can be made open with a screw
driver.
• The scion should be about 10 -13 cm long , 10-
12mm thick and with 2 or 3 healthy vegetative
buds.
85. WEDGE GRAFTING…
• The basal ends of the scion should be cut into
a “V” shaped wedge , matching the opening
in the stock
• The scion is inserted into the “V” shaped
opening in the stock in such a way that the
cambium of the stock and scion are closely
matched .
• All the cut surfaces are covered with grafting
wax.
86. SIDE GRAFTING
• The scion is inserted into the side of the root
stock, which is larger in diameter than the
scion.
Side – stub grafting (Side – wedge grafting)
• Simplest and most effective method
• Useful in branches of trees that are too large
for whip & tongue graft
• Root stocks - branches of about 2.5 cm
87. Side – stub grafting (Side – wedge grafting)
Image:http://himachalfruits.com/
88. Side – stub grafting (Side – wedge grafting)…
• An oblique , 2.5 cm deep cut in the stock at
an angle of 20 ° to 30°.
• Scion- 7.5 cm long, thin, with 2 or 3 buds.
• Base of the scion is cut into a narrow thin
wedge.
• The root stock is then gently bent away from
its side cut so that it opens sufficiently
• The scion is inserted – cambial layer should
match with that of the stock.
89. Side – stub grafting (Side – wedge grafting)…
• The graft is tightly tied with polyethylene
tape to seal the entire area.
• The entire graft union must be completely
covered with grafting wax.
• After the graft is completed, the root stock
may be cut off , just above the union.
91. SIDE – TONGUE GRAFTING…
• Useful for small plants.
• The diameter of the scion should be slightly smaller
than that of root stock.
• A sloping cut is made at the base of the scion. A
second cut is made under the first forming a thin
tongue.
• A cut of similar length is made on the root stock. A
reverse cut is made downward , starting one –third of
the distance from the top of the cut. The second cut
in the root stock should be of the same length as the
reverse cut in the scion.
92. SIDE – TONGUE GRAFTING…
• The scion is inserted into the cut in the root
stock – the two tongues interlocking & the
cambium layers matching along the side.
• The graft is wrapped with plastic tape and
waxed.
• After the graft union is complete, cut the top
of the root stock just above the scion.
94. SIDE - VENEER GRAFTING…
• The thickness of the stock is usually more than
the scion.
• A long shallow cut of 2 – 3 cm long is made on
one side of the stem of root stock.
• A second , short downward cut is made at the
base of the first to remove a piece of bark and a
little wood.
• A long shallow cut is made on one side of the
stem of scion. A second very short cut is made at
the base of the scion on the opposite side.
95. SIDE - VENEER GRAFTING…
• The cuts on the stock and scion should be of
the same length and width.
• The scion is inserted into the stock – the
cambium of stock & scion should match at
least along one side
• Tie with polyethylene tape.
• After the union is complete, the root stock is
cut back, leaving the scion to grow.
97. BARK GRAFTING…
• Done when bark slips readily.
• Stock is larger than scion – sometimes 2 or 3 scions
are placed on large stocks
• Cut stock and the bark is split downwards
from the apex about 5cm long.
• Scion , 12 – 15 cm long and 6 – 12.5 cm thick ,
containing 2 or 3 buds.
• First , a long cut is made on the scion . A second
shorter cut is made on the side opposite to the first
cut , making the basal end of the scion to a wedge
shape
• Insert scion between the bark and wood of the root
stock , placing the longer cut of the scion against the
wood – tie and apply grafting wax on the graft joint.
99. INLAY BARK GRAFT…
• Suitable for thick –barked trees (e.g. Walnut)
where insertion of the scion under bark is not
feasible.
• 2 parallel vertical cuts , 2.5 – 5 cm long are
made through the bark of the root stock down
to the wood. The distance between the 2 cuts
should be equal to the width of the scion
• Terminal two –thirds of this bark is lifted and cut
off, leaving a small flap at the bottom.
100. INLAY BARK GRAFT…
• A 5 cm long slanting cut is made on one side
at the basal end of the scion and a shorter
cut is made on the opposite side forming a
wedge at the base of the scion.
• Scion is inserted into the slot made by the
removal of the bark.
• Secure the graft in position and apply grafting
wax.
102. ROOT GRAFTING…
• Roots are used as root stock & the scion
stem is grafted to it.
1. Whole root graft- whole root system is used
for grafting
2. Piece root graft - small pieces of roots are
used as stocks
Egs : Apple, Pear
103. ROOT GRAFTING…
• Root stock plants are dug and stored under
cool ( 1.5 to 4.5 C °) and moist conditions.
Root pieces should be 7.5 – 15 cm long
• Scion should be of the same length with 2 -4
buds. Usually the scion wood is collected and
stored.
104. ROOT GRAFTING…
• Grafting (Whip & Tongue type is commonly
used) is performed indoors with dormant
scions and root stocks at benches (Hence,
also known as Bench grafting).
• After the grafts are made and properly tied,
they are bundled together in groups of 50 –
100 and stored for callusing in damp sand or
other packing material.
105. II. APPROACH GRAFTING
• Two independent plants are grafted together.
• After the grafting union, the top of the root
stock plant is removed above the graft and
the base of the scion plant is removed below
the graft gradually to prevent the sudden
shock of separation.
Egs: Mango, Sapota, Litchi
108. SPLICED APPROACH GRAFTING…
• Both stock and scion should be of equal
thickness.
• The pot containing the root stock is placed
near the scion desired to be propagated
• A thin slice of bark and wood about 60 – 70
mm long is removed from the stock at a
height of about 25 – 30 cm from the soil
surface.
• A similar cut is made on the scion shoot.
109. SPLICED APPROACH GRAFTING…
• The stock and the scion are held together in
such a way that the cut position fits closely
without any gap between them.
• Tied firmly with jute fibre or wax tape.
• Grafting wax is applied at the graft joint to
prevent the wilting of tissues.
• The union will be completed in about 40 -60
days – After the union ,scion is cut below the
union and the stock above the union, resulting a
new plant consisting of a root stock and a
grafted top.
111. TONGUED APPROACH GRAFTING…
• Same as the spliced approach grafting except
that after the first cut is made in each stem to
be joined, a second cut – downward on the
stock and upward on the scion is made , thus
providing a thin tongue on each.
• By interlocking these tongues, a very tight ,
closely fitting graft union can be observed.
112. III. REPAIR GRAFTING
INARCHING
• Similar to approach grafting
• Used to replace damaged roots
• Seedlings planted beside the damaged tree
are grafted into the trunk of the tree to
provide a new root system
115. REPAIR GRAFTING – BRIDGE GRAFTING…
• Used when there is injury to the trunk.
• Done when active growth of the tree occurs
and the bark is easily slipping.
• The torn or dead bark is removed.
• A scion is inserted every 5 to 7.5 cm around
the injured section and attached at both
upper and lower ends into live undamaged
bark – cut surfaces covered with grafting
wax.
116. BUDDING
• A form
vegetative bud is taken
of grafting in which
from
a single
one plant
(scion) and inserted into the stem tissue of
another (root stock) so that the two will
unite and grow together. The inserted bud
develop into new shoot.
117. AIMS/ OBJECTIVES OF BUDDING
• To perpetuate the clone that can not be
readily reproduced by other methods of
propagation.
• To obtain the good qualities of certain root
stocks – for cold hardiness, disease
resistance, salt tolerance etc.
• For changing the cultivars of established
plants(top – working)
• For hastening the growth of seedling.
118. A method of grafting in
which a root stock from
poted plant is grafted
with the scion from an
adult tree in sidewise
position.
It is very useful in
propagation of Mango
and Sapota.
119. ⦿A grafting in which
tongue shaped cut
is made both scion
and root stock for
proper joining.
120. ⦿It is a grafting method
where a wedge shaped
cut is made at the scion,
a cleft is made on the
stock and the scion is
inserted on the stock.
⦿Eg: Mango, Sapota, Jack.
121. ⦿ It is a grafting where the
scion are grafted on the
top of large stock.
⦿ It is also known as top
working or crown
grafting.
122. ⦿Grafting in which the scion
is inserted into the root
stock in lateral position.
⦿ Here both scion and root
stock are taken from
mature tree.
⦿It is also used for
decorating flowering trees
by adding twigs from
different varieties.
123. ⦿Grafting in which
epicotyl portion of root
stock seedling is replaced
by a young shoot tip .
⦿It is used the
propagation of fruits
such as Mango,
Cashew.
124. ⦿Plants like mango &sapota which cannot be propagated by
cutting,can be propagated by grafting.
⦿High yielding varities are multiplied by grafting.
⦿Disease resistant vsrieties are produced by grafting.
⦿Undesirabe variety can be changed into desirable
variety by grafting.
125. ⦿Vegetative propagation in which a bud is inserted on
to the root stock plant is called budding.
⦿Also known as bud grafting.
⦿Bud is incorporated into the root stock and allowed to
grow further while the buds of the root stock are
removed.There fore, inserted bud alone to produce
the shoot systems.
⦿Bud is used as Scion & it should superior
desired trait.
⦿Parent plant provide root system for survival of scion –
Root stock plant
128. T- BUDDING…
• Done when the stock plant is in active growth
and the cambial cells are actively dividing so
that the bark separates easily from the wood-
slipping
• Shield budding – shield like appearance of
the bud piece from the scion.
129. T- BUDDING - Procedure
• Select stock & scion (bud stick)
• Select a suitable internodal smooth bark (15
-20 cm from the ground level)
• Give a vertical cut , 2.5 – 3.7 cm (bark only)
• At the top of the vertical cut, give another
horizontal cut T –shaped incision.
• Lift the bark piece on either side of the
vertical cut for insertion of bud.
130. T- BUDDING – Procedure…
• The scion bud is removed in the form of a
shield.
• Insert the bud between the flaps of bark on
the stock
• Wrap the bud and stock firmly in such a way
that the bud is fully exposed.
132. INVERTED T – BUDDING…
• Similar to T – budding except that the
horizontal cut is made at the bottom of the
vertical cut.
• Used to prevent the possible entry of water
from the top of the T- cut which may cause
rotting of the shield piece.
135. PATCH BUDDING…
• Done during the period when the bark of
stock and scion slip easily.
• A rectangular patch of bark is completely
removed from the stock – on the stock plant
give 2 transverse cuts – width 1 to 2.5 cm
( only bark deep) parallel to each other and
with a distance of about 2.5 to 3.75 cm
between them.
136. PATCH BUDDING…
• Join the transverse cuts at their ends by two
vertical cuts and remove the patch of bark.
• On the scion, give 2 transverse cuts and
vertical cuts of similar dimension as above
and remove the bark patch with the bud.
• Insert the bud patch on the stock
• Wrap the bud joint with budding tape,
exposing the bud.
138. I – BUDDING…
• Make 2 transverse cuts through the bark of the
root stock
• Join these cuts at their centre by a single
vertical cut → I – shaped incision .
• Cut the bud patch in the form of a rectangle or
square.
• Raise the 2 flaps of bark and insert the bud
patch inside the flaps.
• Tie with budding tape, exposing the bud.
Remove the budding tape when the union is
complete.
142. CHIP BUDDING…
• Done when the bark does not slip well.
• A chip of bark , 2.5 – 3 cm long is removed from
a smooth portion of internode of the stock.
• Another chip of the same size and shape with a
bud is removed from the scion and placed on
the stock.
• Wrap it exposing the bud .
• Stock is cut back when the union is complete
and the bud starts growing .
• Used in Citrus, Apple etc.
145. RING (ANNULAR ) BUDDING…
• Done when the bark slips easily.
• Stock and scion should be of the same diameter
• A ring of bark (1.25 – 2.5 cm) with a bud is
loosened from the scion and slipped off from
one end of the branch.
• The stock is cut back to a height where the
budding is to be done – a portion of the bark is
peeled off and the scion is slipped down over
the stock.
• Wrap with a budding tape , exposing the bud.
147. FLUTE BUDDING…
• Done when the bark slips easily
• Remove the bark encircling the root stock
almost completely , leaving a narrow strip of
bark – on the stock plant, give 2 vertical cuts
(2.5 – 3.75 cm) , parallel to each other and
with a distance of 1/8 of the circumference
of the stock plant .
148. FLUTE BUDDING…
• Joint the ends of these two vertical cuts by 2
parallel horizontal cuts and remove the bark
piece
• Similar cuts are also given in the bud sticks
and remove the bark piece with bud.
• Insert the scion on the stock
• Wrap with budding tape , exposing the bud.
• After the union and the bud starts to grow ,
remove the tape and cut the top of the stock
149. FORKERT BUDDING(FLAP
BUDDING)
• A transverse cut and two vertical cuts joining the transverse cuts
given on the stock and the bark is carefully peeled along these
but remain attached on the lower side in the form of a flap.
• The scion bud of the size corresponding to the cut made on the
stock is removed
• The bud patch is fitted into the exposed portion of the stock .
• The flap of the bark of stock is used to cover the inserted bud p
and remove a little portion to expose the bud and wrapped
budding tape.
150. FORKERT BUDDING…
• When the union is complete, the budding
tape is removed and the flap is cutoff.
• When the bud starts growing, cut the top of
the stock
e.g. Rubber, Teak etc.
152. ⦿Vegetatve propagation in
which a bud is inserted
into t shaped incision
made in the root stock .
⦿Scion appear as shield so
that this method is known
as shield budding.
⦿Oranges, rose, plums,
peaches.
153. ⦿Method of bud grafting in
which a patch of bark with a
bud is inserted into a similar
depatched root stock.
⦿Citrus , mango,
rubber
,annona,wal nut, etc .
154. ⦿The method of bud
grafting in which bud
along with a piece of
wood is inserted in a root
stock .
⦿Grapes.
155. ⦿Bud grafting in which a bud
is inserted into the flap of
bark lifted from rootstock.
⦿Also called forkert budding
since the bark of the
rootstock is gently lifted for
inserting the scion bud.
156. ⦿Bud grating in which a
bud along with a ring of
bark from bud wood is
inserted into the
rootstock .
⦿Ex; chincona
157. ADVANTAGES
It is an effective means of propagating species that usually do not root easily by
cuttings as in mango, kumquat, filberts and litchi.
It is the best method of propagation of plants, which reproduce naturally by
layering e.g., black berries, black raspberries, etc.
It does not require precise control on water, relative humidity or temperature as is
required for other methods of propagation.
It is easy to perform and does not require much facility.
It is possible to produce large sized plant with layering within a short time.
Layering is useful for producing relatively a smaller number of plants of good
size with minimum propagation facilities.
158. DISADVANTAGES
It is a costlier technique in areas where labour availability is problem.
It is not possible to produce large number of plants within short time.
The plants produced through layering have usually small and brittle roots.
In layering, the beneficial effects of rootstocks on the scion cultivar can’t
be exploited.
The mortality rate in layers (particularly air layers) is usually high.