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Priyanka P. Parab
B.Sc. Agriculture
Methods of Propagation :- Grafting and
Budding
What Is a Graft?
Grafting and budding are methods of asexual
plant propagation that join parts from two
different plants so they will grow as one plant.
Why do we do graft?
Propagate the plants where other methods would
not worked
Change cultivar on established plant
Repair damage tree parts
Faster production of new fruit 2/3 vs 5/7 years
Novelties – more than one cultivar on one tree
Grafting terms
Grafting: Joining two plant pieces to make one
plant
Scion: Detached shoot from last year’s growth
with dormant buds, upper graft part.
Stock: Basal part of the graft (understock or
rootstock)
Interstock: Stem pieces added between stock &
scion
Cambium: This is a single layer of cells between
the wood and bark. It must be lined up for a
good graft union.
Steps in Healing
Tissues involved are the Xylem, Phloem and
Cambium
Callus from stock & scion fill the space and interlock
to form “callusbridge”
Callus cells in line between stock & scion cambium
change into cambium cells
New cambium produce Xylem & Phloem in wound
to establish a vascular connection.
Tissue involved in graft union
Tools used
Budding /Grafting Knife
Pruning shear
Tying materials:- Tape,
Rubber strips, polythene
strips
Heavy knife
wax
Types of Grafting
1) Veneer grafting
2) Whip grafting
3) Tongue grafting
4) Cleft grafting
5) Wedge grafting
6) Bridge grafting
7) Epicotyl grafting
8) Soft wood grafting
9) Inarching
10)Double working
11)Top working
12)Frame working
Grafting Machine
Epicotyl & Softwood grafting in Mango
Field Preparation for Mango
Dig Pits of 1m X 1m X 1m
Fill with top soil mixed with 10kg FYM
and 100g Lindane 1.3% dust per pit
Planting Mango Graft
One year old Grafts are planted in the centre of
the pit with ball of earth intact followed by
watering and staking.
The graft union must be 15cm above the
ground level
The spacing followed in mango orchard is 10 X
10m, in high density planting that can be
reduced to 6m X 6m/ 5m X 5m
Manures and Fertilizers
Manures and fertilizers are applied in September –
October
Fertilizers are applied towards the peripheral area of
the canopy
Fertilizer application:- 170 gm Urea, 110gm Single
Super Phosphate and 115gm
Muriate of potash per plant per year from first to tenth
year and thereafter 1.7 kg, 1.1Kg, and 1.15kg respectively
of these fertilizer per plant per year can be applied in
split doses (June- July and october)
Budding
It is the form of grafting in which one bud and
a small section of bark with or without wood is
used.
Chip budding and T-budding are the most
important types of budding for fruit crops and
woody ornamentals.
Types of Budding
1. Shield budding or T-budding
2.Patch budding
3.Chip budding
4.Ring budding
5.Modified ring budding
T-Budding
Budding
Modified Ring
Budding
Chip Budding
Grafting and Budding
Precautions
Cambial layers of stock and scion must meet
Parts must be held securely keep air out —
Union heals by callus production Adequate
temperature for cell division There are limitations!
All the equipment's should be well sterilized
Layering
It is the form of rooting of cuttings in which
adventitious roots are initiated on a stem(layer)
is then detached, transplanted, while later
becomes a separate plant on its own roots.
Air layering is mostly preferred in
Sapota(chickoo), Guava,etc.
Advantages of Layering
a) It is an effective method of propagating species that
usually do not root easily by cuttings as in mango,
litchi, sapota, guava, etc.
b)It does not require precise control on water, relative
humidity, or temperature.
c) Easy-to-perform and does not require much
infrastructure.
d)It is nature method of propagation in Blackberries and
Raspberries.
Types of Layering
1. Simple layering
2. Serpentine/Compound layering
3. Mound layering or Stooling
4. Trench/Continuous layering
5. Tip layering
6. Air layering
Air Layering
Continuous Layering Simple Layering and
Stooling
Thank You
priyankaparab21p3@gmail.com
Contact No. 7507887303
Grafting methods.pptx

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Grafting methods.pptx

  • 1. Priyanka P. Parab B.Sc. Agriculture Methods of Propagation :- Grafting and Budding
  • 2. What Is a Graft? Grafting and budding are methods of asexual plant propagation that join parts from two different plants so they will grow as one plant.
  • 3. Why do we do graft? Propagate the plants where other methods would not worked Change cultivar on established plant Repair damage tree parts Faster production of new fruit 2/3 vs 5/7 years Novelties – more than one cultivar on one tree
  • 4. Grafting terms Grafting: Joining two plant pieces to make one plant Scion: Detached shoot from last year’s growth with dormant buds, upper graft part. Stock: Basal part of the graft (understock or rootstock)
  • 5. Interstock: Stem pieces added between stock & scion Cambium: This is a single layer of cells between the wood and bark. It must be lined up for a good graft union.
  • 6. Steps in Healing Tissues involved are the Xylem, Phloem and Cambium Callus from stock & scion fill the space and interlock to form “callusbridge” Callus cells in line between stock & scion cambium change into cambium cells New cambium produce Xylem & Phloem in wound to establish a vascular connection.
  • 7. Tissue involved in graft union
  • 8. Tools used Budding /Grafting Knife Pruning shear Tying materials:- Tape, Rubber strips, polythene strips Heavy knife wax
  • 9. Types of Grafting 1) Veneer grafting 2) Whip grafting 3) Tongue grafting 4) Cleft grafting 5) Wedge grafting 6) Bridge grafting 7) Epicotyl grafting 8) Soft wood grafting 9) Inarching 10)Double working 11)Top working 12)Frame working
  • 10.
  • 12.
  • 13.
  • 14.
  • 15.
  • 16.
  • 17.
  • 18. Epicotyl & Softwood grafting in Mango
  • 19. Field Preparation for Mango Dig Pits of 1m X 1m X 1m Fill with top soil mixed with 10kg FYM and 100g Lindane 1.3% dust per pit
  • 20. Planting Mango Graft One year old Grafts are planted in the centre of the pit with ball of earth intact followed by watering and staking. The graft union must be 15cm above the ground level The spacing followed in mango orchard is 10 X 10m, in high density planting that can be reduced to 6m X 6m/ 5m X 5m
  • 21. Manures and Fertilizers Manures and fertilizers are applied in September – October Fertilizers are applied towards the peripheral area of the canopy Fertilizer application:- 170 gm Urea, 110gm Single Super Phosphate and 115gm Muriate of potash per plant per year from first to tenth year and thereafter 1.7 kg, 1.1Kg, and 1.15kg respectively of these fertilizer per plant per year can be applied in split doses (June- July and october)
  • 22. Budding It is the form of grafting in which one bud and a small section of bark with or without wood is used. Chip budding and T-budding are the most important types of budding for fruit crops and woody ornamentals.
  • 23. Types of Budding 1. Shield budding or T-budding 2.Patch budding 3.Chip budding 4.Ring budding 5.Modified ring budding
  • 26.
  • 28. Grafting and Budding Precautions Cambial layers of stock and scion must meet Parts must be held securely keep air out — Union heals by callus production Adequate temperature for cell division There are limitations! All the equipment's should be well sterilized
  • 29. Layering It is the form of rooting of cuttings in which adventitious roots are initiated on a stem(layer) is then detached, transplanted, while later becomes a separate plant on its own roots. Air layering is mostly preferred in Sapota(chickoo), Guava,etc.
  • 30. Advantages of Layering a) It is an effective method of propagating species that usually do not root easily by cuttings as in mango, litchi, sapota, guava, etc. b)It does not require precise control on water, relative humidity, or temperature. c) Easy-to-perform and does not require much infrastructure. d)It is nature method of propagation in Blackberries and Raspberries.
  • 31. Types of Layering 1. Simple layering 2. Serpentine/Compound layering 3. Mound layering or Stooling 4. Trench/Continuous layering 5. Tip layering 6. Air layering
  • 33.
  • 34. Continuous Layering Simple Layering and Stooling
  • 35.