This document provides instructions for surface sterilization of plant cultures. It discusses: 1) Explants must be sterilized to prevent microbial contamination that can kill cultured tissues. 2) Surface sterilization methods include rinsing explants, soaking in detergent and disinfectants like ethanol or mercuric chloride, then rinsing in sterile water. 3) Non-living materials like media and glassware are sterilized by autoclaving, while filters sterilize thermolabile compounds. Proper sterilization is essential to increase success of tissue culture experiments.