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This practical aims to induce callus formation from different plant explants. Students will take explants like leaf, stem, and root segments from selected horticultural crops and culture them on nutrient media supplemented with plant growth regulators, like auxins and cytokinins, to induce callus tissue. The success of callus induction will depend on the explant type and plant species as well as the plant growth regulator concentrations used in the culture medium.








































