Food spoils due to the presence of microorganisms like bacteria and fungi, as well as enzymes. When bread or chapatis are left in open air, a fungal or bacterial growth appears. Food stored in copper pots or milk left in open air can also spoil due to bacteria or metallic reactions over long periods. Microorganisms require moisture and oxygen to rapidly multiply and cause spoilage. Various preservation methods aim to prevent or slow the growth of these microorganisms and enzymes in order to prolong the shelf life of foods.