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FOOD SPOILAGE:
Fruits and Vegetables
• Spoilage organisms:
 Yeasts: target fruits and vegetables due to their low pH
 Moulds: can grow even at very low water activity
 Bacteria: most bacteria have limited pH, prolytic bacteria can grow even in media with
high pH
• Factors that influence microbial activity:
 Water activity: lower water activity inhibits microbial growth
 pH: most microorganisms have limited pH range
 Nutrient contents: growth rate faster is nutrients abundant
 Temperature: lower temperature retards microbial growth
• Stages in spoilage of fruits:
 Usually fruits inhibit the growth of microbes until after harvest
 Ripening weakens cell walls and decreases the amount of antifungal chemicals
 Physical damage during harvesting breaks outer protective layers
 Fruits have a low pH that inhibits most bacteria, except acid tolerant bacteria (Gram
positive lactobacilli & leuconostocs)
 Molds are tolerant of acidic conditions and are involved in the spoilage of citrus fruits,
apples, pears etc.
• Stages of spoilage in vegetables:
 Vegetables are a tempting source of nutrients for spoilage organisms due to their near
neutral pH and high water activity
 Bacterial spoilage first causes softening of tissues as pectins are degraded and the whole
vegetable eventually degenerates into a slimy mass
 Starches and sugars are metabolized next and unpleasant odors and flavors develop
along with lactic acid and ethanol
• Preservation techniques include:
 Refrigeration
 Canning
 Pasteurization
 Drying
 Irradiation
 Anti-microbial compounds: nisin, chitosan
SOME MICROBIAL DEFECTS OF FRUITS
SOME MICROBIAL DEFECTS OF VEGETABLES

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Microbial Spoilage of fruits and vegetables

  • 2. • Spoilage organisms:  Yeasts: target fruits and vegetables due to their low pH  Moulds: can grow even at very low water activity  Bacteria: most bacteria have limited pH, prolytic bacteria can grow even in media with high pH • Factors that influence microbial activity:  Water activity: lower water activity inhibits microbial growth  pH: most microorganisms have limited pH range  Nutrient contents: growth rate faster is nutrients abundant  Temperature: lower temperature retards microbial growth
  • 3. • Stages in spoilage of fruits:  Usually fruits inhibit the growth of microbes until after harvest  Ripening weakens cell walls and decreases the amount of antifungal chemicals  Physical damage during harvesting breaks outer protective layers  Fruits have a low pH that inhibits most bacteria, except acid tolerant bacteria (Gram positive lactobacilli & leuconostocs)  Molds are tolerant of acidic conditions and are involved in the spoilage of citrus fruits, apples, pears etc. • Stages of spoilage in vegetables:  Vegetables are a tempting source of nutrients for spoilage organisms due to their near neutral pH and high water activity  Bacterial spoilage first causes softening of tissues as pectins are degraded and the whole vegetable eventually degenerates into a slimy mass  Starches and sugars are metabolized next and unpleasant odors and flavors develop along with lactic acid and ethanol • Preservation techniques include:  Refrigeration  Canning  Pasteurization  Drying  Irradiation  Anti-microbial compounds: nisin, chitosan
  • 5. SOME MICROBIAL DEFECTS OF VEGETABLES