The fluorescence microscope uses fluorescence and phosphorescence instead of reflection and absorption to study organic and inorganic substances. It has a UV light source and filter to protect the viewer. Some organisms and substances naturally fluoresce, while others can be stained with fluorescent dyes. The fluorescence microscope is used widely in diagnostic microbiology to detect antigens, antibodies, and nucleic acids. Electron microscopes have much higher resolving power than light microscopes due to the small wavelength of electrons. Transmission electron microscopes allow viewing of inner structures while scanning electron microscopes image surfaces. Both have various applications in biology and medicine.
Electron microscopy by SIVASANGARI SHANMUGAM.
Electron microscopy is a technique for obtaining high-resolution images of biological and non-biological specimens.
Electron microscopy by SIVASANGARI SHANMUGAM.
Electron microscopy is a technique for obtaining high-resolution images of biological and non-biological specimens.
SEM is a type of electron microscope designed for directly studying the surfaces of solid objects, that utilizes a beam of focused electron of relatively low energy as an electron probe that is scanned in a regular manner over the specimen.
scanning electron microscope for analysisM Ali Mohsin
SEM stands for scanning electron microscope. The SEM is a microscope that uses electrons instead of light to form an image. Since their development in the early 1950's, scanning electron microscopes have developed new areas of study in the medical and physical science communities.
Electron microscopy (EM) is a technique for obtaining high resolution images of biological and non-biological specimens. It is used in biomedical research to investigate the detailed structure of tissues, cells, organelles and macromolecular complexes
SEM is a type of electron microscope designed for directly studying the surfaces of solid objects, that utilizes a beam of focused electron of relatively low energy as an electron probe that is scanned in a regular manner over the specimen.
scanning electron microscope for analysisM Ali Mohsin
SEM stands for scanning electron microscope. The SEM is a microscope that uses electrons instead of light to form an image. Since their development in the early 1950's, scanning electron microscopes have developed new areas of study in the medical and physical science communities.
Electron microscopy (EM) is a technique for obtaining high resolution images of biological and non-biological specimens. It is used in biomedical research to investigate the detailed structure of tissues, cells, organelles and macromolecular complexes
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Phenomics assisted breeding in crop improvementIshaGoswami9
As the population is increasing and will reach about 9 billion upto 2050. Also due to climate change, it is difficult to meet the food requirement of such a large population. Facing the challenges presented by resource shortages, climate
change, and increasing global population, crop yield and quality need to be improved in a sustainable way over the coming decades. Genetic improvement by breeding is the best way to increase crop productivity. With the rapid progression of functional
genomics, an increasing number of crop genomes have been sequenced and dozens of genes influencing key agronomic traits have been identified. However, current genome sequence information has not been adequately exploited for understanding
the complex characteristics of multiple gene, owing to a lack of crop phenotypic data. Efficient, automatic, and accurate technologies and platforms that can capture phenotypic data that can
be linked to genomics information for crop improvement at all growth stages have become as important as genotyping. Thus,
high-throughput phenotyping has become the major bottleneck restricting crop breeding. Plant phenomics has been defined as the high-throughput, accurate acquisition and analysis of multi-dimensional phenotypes
during crop growing stages at the organism level, including the cell, tissue, organ, individual plant, plot, and field levels. With the rapid development of novel sensors, imaging technology,
and analysis methods, numerous infrastructure platforms have been developed for phenotyping.
hematic appreciation test is a psychological assessment tool used to measure an individual's appreciation and understanding of specific themes or topics. This test helps to evaluate an individual's ability to connect different ideas and concepts within a given theme, as well as their overall comprehension and interpretation skills. The results of the test can provide valuable insights into an individual's cognitive abilities, creativity, and critical thinking skills
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Nucleophilic Addition of carbonyl compounds.pptxSSR02
Nucleophilic addition is the most important reaction of carbonyls. Not just aldehydes and ketones, but also carboxylic acid derivatives in general.
Carbonyls undergo addition reactions with a large range of nucleophiles.
Comparing the relative basicity of the nucleophile and the product is extremely helpful in determining how reversible the addition reaction is. Reactions with Grignards and hydrides are irreversible. Reactions with weak bases like halides and carboxylates generally don’t happen.
Electronic effects (inductive effects, electron donation) have a large impact on reactivity.
Large groups adjacent to the carbonyl will slow the rate of reaction.
Neutral nucleophiles can also add to carbonyls, although their additions are generally slower and more reversible. Acid catalysis is sometimes employed to increase the rate of addition.
Remote Sensing and Computational, Evolutionary, Supercomputing, and Intellige...University of Maribor
Slides from talk:
Aleš Zamuda: Remote Sensing and Computational, Evolutionary, Supercomputing, and Intelligent Systems.
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Inter-Society Networking Panel GRSS/MTT-S/CIS Panel Session: Promoting Connection and Cooperation
https://www.etran.rs/2024/en/home-english/
ESR spectroscopy in liquid food and beverages.pptxPRIYANKA PATEL
With increasing population, people need to rely on packaged food stuffs. Packaging of food materials requires the preservation of food. There are various methods for the treatment of food to preserve them and irradiation treatment of food is one of them. It is the most common and the most harmless method for the food preservation as it does not alter the necessary micronutrients of food materials. Although irradiated food doesn’t cause any harm to the human health but still the quality assessment of food is required to provide consumers with necessary information about the food. ESR spectroscopy is the most sophisticated way to investigate the quality of the food and the free radicals induced during the processing of the food. ESR spin trapping technique is useful for the detection of highly unstable radicals in the food. The antioxidant capability of liquid food and beverages in mainly performed by spin trapping technique.
2. The Fluorescence Microscope
British scientist Sir George G. Stokes first described
fluorescence in 1852
A fluorescence microscope is an optical microscope
that uses fluorescence and phosphorescence instead
of, or in addition to, reflection and absorption to
study properties of organic or inorganic substances.
Fluorescence : is the emission of light by a substance
that has absorbed light or other electromagnetic
radiation.
3.
4. Fluorescence microscopy is a specially modified
compound microscope furnished with an ultraviolet
(UV) radiation source and a filter that protects the
viewer’s eye from injury by these dangerous rays.
Some organisms fluoresce naturally because of the
presence within the cells of naturally fluorescent
substances such as chlorophyll.
Those that do not naturally fluoresce may be
stained with a group of fluorescent dyes called
fluorochromes.
5. The name of this type of microscopy originates
from certain dyes (acridine, fluorescein) and
minerals that possess the property of
fluorescence.
This means that they emit visible light when
bombarded by shorter UV rays.
This has been widely used in diagnostic
microbiology to detect both the antigen and
antibodies, may they be in pure form or mixed
form.
6. Principle
The fluorescence microscope depends on two
intrinsic properties of the substance to be
observed
– FLUORESCENCE
– PHOSPHORESCENCE
7. Applications of fluorescence microscope
in clinical samples?
1) Fluorescent staining is commonly used to improve
tuberculosis diagnosis efficiency as well as for
malaria diagnosis.
2) Early detection of bacteria in blood cultures, and to
detect and identify nucleic acids by color.
3) Chromosomal anomalies ( FISH)
4) Fluorescent antibodies provide a wide variety of
immunologically specific, rapid diagnostic tests for
infectious diseases. can observe in live cells
8. ELECTRON MICROSCOPY
In 1938 Von Borries and Ruska built the first
practical electron microscope.
The electron microscope use electron beams
and magnetic fields to produce the image
instead of light waves and glass lenses used
in the light microscopes.
Resolving power of electron microscope is far
greater than that of any other compound
microscope. This is due to shorter
wavelengths of electrons. The wavelength of
electrons are about 100,000 times smaller
than the wavelength of visible light.
9.
10.
11. Method For Electron
Microscope
The specimen to be observed is prepared as
extremely thin dry film on small screens.
These are then introduced into the instrument at
a point between the magnetic condenser and
the magnetic objective.
The magnified image is viewed on a fluorescent
screen through an airtight window.
The image can be recorded on a photographic
plate by a camera built into the instrument.
12. Types of electron microscopy
Mainly 2 types:
1) Transmission Electron Microscope
(TEM) - allows one the study of the
inner structures.
2) Scanning Electron Microscope (SEM) -
used to visualize the surface of objects.
13. PRINCIPLE OF WORKING OF TEM
Electrons possess a wave like character.
Electrons emitted into vacuum from a
heated filament with increased
accelerating potential will have small
wavelength.
Such higher-energy electrons can
penetrate distances of several microns
into a solid.
If these transmitted electrons could be
focused - images with much better
resolution.
Focusing relies on the fact that, electrons
also behave as negatively charged
particles and are therefore deflected by
electric or magnetic fields.
14. What is SEM?
The scanning electron microscope (SEM) uses
a focused beam of high-energy electrons to
generate a variety of signals at the surface of
solid specimens. The signals that derive from
electron-sample interactions reveal information
about the sample.
15. PRINCIPLE OF SEM
Accelerated electrons in an
SEM carry significant
amounts of kinetic energy,
and this energy is dissipated
as a variety of signals
produced by electron-sample
interactions when the incident
electrons are decelerated in
the solid sample.
These signals include
secondary electrons that
produce SEM images.
16. SEM WORKING
The electron gun produces an electron beam which is
accelerated by the anode.
The beam travels through electromagnetic fields and lenses,
which focus the beam down toward the sample.
A mechanism of deflection coils enables to guide the beam
so that it scans the surface of the sample in a rectangular
frame.
When the beam touches the surface of the sample, it
produces:
– Secondary electrons (SE)
– Back scattered electrons (BSE)
– X - Rays...
The emitted SE is collected by SED and convert it into signal
that is sent to a screen which produces final image.
17.
18. Differences between SEM and
TEM
TEM SEM
1. Electron beam passes through thin
sample.
1. Electron beam scans over surface of
sample.
2. Specially prepared thin samples are
supported on TEM grids
2. Sample can be any thickness and is
mounted on an aluminum stub.
3. Specimen stage halfway down
column.
3. Specimen stage in the chamber at the
bottom of the column.
4. Image shown on fluorescent screen. 4. Image shown on TV monitor.
5. Image is a two dimensional projection of
the sample.
5. Image is of the surface of the sample
19. ADVANTAGES & DISADVANTAGES OF TEM
Advantages:
1) TEMs offer very powerful magnification and resolution.
2) TEMs have a wide-range of applications and can be utilized
in a variety of different scientific, educational and industrial
fields
3) TEMs provide information on element and compound
structure .
4) Images are high-quality and detailed.
Disadvantages:
1) TEMs are large and very expensive.
2) Laborious sample preparation.
3) Operation and analysis requires special training.
4) Samples are limited to those that are electron transparent.
5) TEMs require special housing and maintenance.
6) Images are black and white .
20. BIOLOGICAL APPLICATIONS OF TEM
1) In medicine as a diagnostic tool – important in
renal biopsies.
2) Cellular tomography, Used for obtaining
detailed 3D structures of subcellular
macromolecular objects.
3) Cancer research - studies of tumor cell
ultrastructure .
4) Toxicology – to study the impacts of
environmental pollution on the different levels of
biological organization.
21. ADVANTAGES & DISADVANTAGES OF SEM
Advantages
1) It gives detailed 3D and topographical imaging and the
versatile information garnered from different detectors.
2) This instrument works very fast.
3) Modern SEMs allow for the generation of data in digital form.
4) Most SEM samples require minimal preparation actions.
Disadvantages
1) SEMs are expensive and large.
2) Special training is required to operate an SEM.
3) The preparation of samples can result in artifacts.
4) SEMs are limited to solid samples.
5) SEMs carry a small risk of radiation exposure associated
with the electrons that scatter from beneath the sample
surface.
22. BIOLOGICAL APPLICATIONS OF SEM
1) Virology - for investigations of virus structure
2) Cryo-electron microscopy – Images can be made of
the surface of frozen materials.
3) 3D tissue imaging -
– Helps to know how cells are organized in a 3D
network
4) Forensics - SEM reveals the presence of materials on
evidences that is otherwise undetectable
5) SEM renders detailed 3-D images
A. – extremely small microorganisms
B. – anatomical pictures of insect, worm, spore, or other
organic structures