This document discusses fermentation design and types. It begins by defining fermentation as the process of growing microorganisms in a nutrient media to produce desired end products like food, alcohol, and pharmaceuticals. There are two main types of fermentation - surface fermentation using tray or packed bed fermenters, and submerged fermentation using stirred tank, airlift, or bubble column fermenters. The document outlines the design considerations for fermenters including maintaining favorable growth conditions and ends by comparing batch and continuous fermentation processes.
Steps involved in fermentation products producing a viable product output.various steps and process were explained in them. A semester syllabus of undergraduate microbiology student in his/her semester -5 in paper -6 . I think this might be helpful to you and have a good response after reading this .thank you.
Steps involved in fermentation products producing a viable product output.various steps and process were explained in them. A semester syllabus of undergraduate microbiology student in his/her semester -5 in paper -6 . I think this might be helpful to you and have a good response after reading this .thank you.
Introduction :
Antibiotics are antimicrobial agents produced naturally by other microbes (usually fungi or bacteria)
The first antibiotic was discovered in 1896 by Ernest Duchesne and in 1928 "rediscovered" by Alexander Fleming from the filamentous fungus Penicilium notatum.
The antibiotic substance, named penicillin, was not purified until the 1940s (by Florey and Chain), just in time to be used at the end of the second world war.
Penicillin was the first important commercial product produced by an aerobic, submerged fermentation
Fermentation
Scale up of fermentation
Steps in scale up
Scale up fermentation process
Optimizing scale up of fermentation process
Rules followed while doing scale up
Studies carried out during scale up
Reference
Batch and Continuous Sterilization of Media in Fermentation Industry Dr. Pavan Kundur
Continuous sterilization is the rapid transfer of heat to medium through steam condensate without the use of a heat exchanger. ... This is more efficient than batch sterilization because instead of expending energy to heat, hold, and cool the entire system, small portions of the inlet streams are heated at a time.
The material describes components of industrial fermentation media with their respective metabolic importance for the industrial microbes. it also addresses industrial scale sterilization methods.
BIOTECHNOLOGY IS CHALLENGING SUBJECT TO TEACH AND UNDERSTAND ALSO .....THEIR INTERESTING PART IS TO LEARN ABOUT IMMUNITY AND THE IMPORTANT PART MAJOR COMPATIBILITY COMPLEX
Introduction :
Antibiotics are antimicrobial agents produced naturally by other microbes (usually fungi or bacteria)
The first antibiotic was discovered in 1896 by Ernest Duchesne and in 1928 "rediscovered" by Alexander Fleming from the filamentous fungus Penicilium notatum.
The antibiotic substance, named penicillin, was not purified until the 1940s (by Florey and Chain), just in time to be used at the end of the second world war.
Penicillin was the first important commercial product produced by an aerobic, submerged fermentation
Fermentation
Scale up of fermentation
Steps in scale up
Scale up fermentation process
Optimizing scale up of fermentation process
Rules followed while doing scale up
Studies carried out during scale up
Reference
Batch and Continuous Sterilization of Media in Fermentation Industry Dr. Pavan Kundur
Continuous sterilization is the rapid transfer of heat to medium through steam condensate without the use of a heat exchanger. ... This is more efficient than batch sterilization because instead of expending energy to heat, hold, and cool the entire system, small portions of the inlet streams are heated at a time.
The material describes components of industrial fermentation media with their respective metabolic importance for the industrial microbes. it also addresses industrial scale sterilization methods.
BIOTECHNOLOGY IS CHALLENGING SUBJECT TO TEACH AND UNDERSTAND ALSO .....THEIR INTERESTING PART IS TO LEARN ABOUT IMMUNITY AND THE IMPORTANT PART MAJOR COMPATIBILITY COMPLEX
The function of the fermenter or bioreactor is to provide a suitable environment in which an organism can efficiently produce a target product—the target product might be cell biomass,metabolite and bioconversion Product. It must be so designed that it is able to provide the optimum environments or conditions that will allow supporting the growth of the microorganisms. The design and mode of operation of a fermenter mainly depends on the production organism, the optimal operating condition required for target product formation, product value and scale of production.
The choice of microorganisms is diverse to be used in the fermentation studies. Bacteria, Unicellular fungi, Virus, Algal cells have all been cultivated in fermenters. Now more and more attempts are tried to cultivate single plant and animal cells in fermenters. It is very important for us to know the physical and physiological characteristics of the type of cells which we use in the fermentation. Before designing the vessel, the fermentation vessel must fulfill certain requirements that is needed that will ensure the fermentation process will occur efficiently. Some of the actuated parameters are: the agitation speed, the aeration rate, the heating intensity or cooling rate, and the nutrients feeding rate, acid or base valve. Precise environmental control is of considerable interest in fermentations since oscillations may lower the system efficiency, increase the plasmid instability and produce undesirable end products.
This ppt is prepared by Sandeep Kumar Maurya , m. pharma ,department of pharmaceutical sciences, dr. harisingh gour university sagar madhya pradesh. contains fermentation technology.
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
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Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
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Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
The prostate is an exocrine gland of the male mammalian reproductive system
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Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
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Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Couples presenting to the infertility clinic- Do they really have infertility...
Fermentation design & types
1. FERMENTATION-
DESIGN & TYPES
Mr. Dilip O. Morani.
Asst. Prof.,
Shri D. D. Vispute College of Pharmacy
and Research Center, Panvel.
2. FERMENTATION
Fermentation is the process of growing
microorganisms in a nutrient media by
maintaining physico- chemical
conditions and thereby converting feed
into a desired end product
Fermentation technology is the use of
organisms to
pharmaceuticals
produce
and
food,
alcoholic
beverages on a large scale industrial
basis.
4. DESIGN OF FERMENTER A fermentation process requires a fermenter for successful production
.
Fermentor is the large vessel containing considerable quantities of
nutrient media by maintaining favourable conditions.
The design and nature of the fermentor varies depending upon the
type of fermentation carried out. Invariably all the fermentors provide
the following facilities for the process such as
contamination free environment,
specific temperature maintenance,
maintenance of agitation and aeration, pH control,
monitoring Dissolved Oxygen (DO),
ports for nutrient and reagent feeding (antifoam agents, alkali or
acid),
ports for inoculation and sampling,
provide all aseptic conditions at the time of sample withdrawal
and addition of innoculum
complete removal of broth from the tank and should be easy to
clean
It should be designed in such away that it consumes less power,
have less evaporation, can be used for long periods of operation
6. TYPES OF FERMENTER
Available in various sizes
According to the sizes classified as
Small lab and research fermenter :1-50L
Pilot plant fermenter: 50-1000 L
Large size industrial production scale fermenter: more than 1000 L
Broadly fermentes are also claified as
I. surface fermenters
Tray fermenter
Packed bed column fermenter
II. Submerged fermenters
Simple fermenters (batch and continuous)
Fed batch fermenter
Air-lift
Bubble fermenter
Cyclone column fermenter
Tower fermenter
Other more advanced systems, etc
7. TYPES OF FERMENTER
Surface fermenters
Microbial cells cultured on surface layer of
the nutrient medium (solid/liquid) held in dish
or tray
Aspergillus niger and nicotinic
Used for production of citric acid
acid
from
from
Aspergillus terrus
Microbial films can be developed on the
surfaces of suitable packing medium, may be
in the form of fixed bed, stones or plastic
sheets
8. TRAY FERMENTER
TRAY FERMENTER
one of the simplest and widely used fermenters.
Its basic part is a wooden, metal, or plastic tray, often with
a perforated or wire mesh bottom to improve air circulation.
A shallow layer of less than 0.15 m deep, pretreated
substrate is placed on the tray for fermentation.
Temperature and humidity-controlled chambers are used
for keeping the individual trays or stacks.
A spacing of at least one tray height is usually allowed
between stacked trays.
Cheesecloth may be used to cover the trays to reduce
contamination.
Inoculation and occasional mixing are done manually,
often by hand.
9. TRAY
FERMENTER
•Solid as well as liquid
medium are used
•If liquid medium, cells are
allowed to float easily and to
make a process continuous
•If solid medium is used the
micro-organisms are
allowed grow on moist solid
materials, process is called
Solid State Fermentation
10. SOLID STATE FERMENTATION (SSF)
Solid State Fermentation Method (SSF)
SSF defined as the growth of the micro-organisms on
(moist) solid material in the absence or near-absence
of free water
Used for production of antibiotics, enzymes, alkaloids,
organic acids bio-pharmaceutical products
yields than submerged liquid
Advantages :
• Produce higher
fermentation
• Possibilities of contamination by bacteria and yeast
is very less
• All natural habitats of fungi are easily maintained in
SSF
• culture media very simple , provides all nutrients for
growth of micro-organisms
11. SSF
Disadvantages:
•Causes problems in monitoring of the process parameters such
as pH, moisture content, and oxygen concentration
•Despite some automation, tray fermenters are
labor intensive
•Difficulties with processing hundreds of trays limit their
scalability
•Aeration may be difficult due to high level of solid content
•Substrates require pre treatment such as size reduction,
chemical or enzymatic hydrolyses
12. SUBMERGED FERMENTERS
The microorganisms are dispersed in liquid
nutrient medium at maintained environmental
conditions.
on the mechanism of agitation Submerged
fermenters grouped as follows:
I. Mechanically stirred fermenter
○ batch operate fermenter
○ continuous stirred tank fermenter
II. Forced convection fermenters
○ Air –lift fermenter
○ Bubble column
○ Sparged tank fermenter
III. Pneumatic fermenter
○ Fluidized bed reactor
13. These are equipped with a
mechanical agitator so as to maintain
homogencity and rapid dispersion
and mixing of materials
stirred tank Examples includes
fermenter (batch or continuous
operated) , multistage fermenter,
paddle wheel reactor, and stirred loop
reactor
MECHANICALLY STIRRED
FERMENTER
14. STIRRED TANK
FERMENTER (STF)
stirred tank fermenter
batch operated
fermenter
agitators consists of one
or more impellers
mounted on the shaft
It is rotates with the help
of electric motor
of this
flexibility in
Advantage
fermenter
design
Used in the range of 1-
100 ton capacity sizes Stirred tank fermenter
15. A continuous stirred
tank fermenter consists
of a cylindrical vessel
with motor driven
central shaft that
supports one or more
agitators (impellers).
The shaft is fitted at
the top of the
bioreactor (ref. fig.).
The number of
impellers is variable
and depends on the
size of the fermenter
CONTINUOUS STIRRED TANK
FERMENTER (CSTF)
Continuous stirred tank
fermenter
16. CONTINUOUS
STIRRED TANK
FERMENTER
In this fresh medium is added continuously in
the fermenter vessel
On the other end the medium is withdrawn for
the recovery of fermentation products
As it is a continuous fermenter the Steady state
conditions can be achieved by either
Chemostatic or Turbidostatic principles.
17. CONTINUOUS STIRRED
TANK FERMENTER(CSTF)
Different types of continuous fermenter
are
a.Single stage: single fermenter is
inoculated and kept in continuous
operation by balancing the input and
output culture media
b. Recycle continuous fermentation: a
residual unused substrate plus
portion of the withdrawn culture or
the
withdrawn culture is recycled
19. STF
Advantages of batch operated
Less risk of contamination because of short
growth period
Process is more economical and simple
Raw material conversion level is high
Disadvantages:
Low productivity due to time required fro
the sterilizing, filling, cooling, emptying and
cleaning
subcultures for inoculation, labor
More expenses are required for
and
process control
20. STF
Advantages of continuous operated
Less labor expenses due to automation of
fermentation process
Less toxicity risk to operator by toxins producing
microorganisms
High yield and good quality product due invariable
operating parameters and automation of the
process
Less stress on the fermenter as sterilization is not
frequent
Disadvantages:
Higher investment costs in control and automation
equipment
More risk of contamination and cell mutation
21. AIR LIFT
FERMENTER
Airlift fermenter (ALF) is generally
classified as forced convection
fermenters without any mechanical
stirring arrangements for mixing.
The turbulence caused by the fluid
(air/gas) flow ensures adequate
mixing of the liquid. The baffle or
draft tube is provided in the reactor.
A baffle or draft tube divides the
fluid volume of the vessel into 2
inter-connected zones.
Only one of the 2 zones is sparged
with air or other gas.
The sparged zone is known as "
riser", the zone that receives no gas
is "downcomer“.
Air lift fermenter
22. AIR LIFT
FERMENTER
Mainly 2 types
Internal-loop airlift bioreactor (ref
Fig) has a single container with a
central draft tube that creates
interior liquid circulation channels.
These bioreactors are simple in
design, with volume and circulation
at a fixed rate for fermentation.
External loop airlift bioreactor (ref
fig) possesses an external loop so
that the liquid circulates
through
independent
separate
channels.
These reactors can be
suitably modified to suit the
requirements of different
fermentations.
Internal loop External loop
23. AIR LIFT
FERMENTER
Advantages
The airlift bioreactors are more efficient than
bubble columns, particularly for more denser
suspensions of microorganisms as the mixing of
the contents is better compared to bubble
columns.
Commonly employed for aerobic bioprocessing
technology.
They ensure a controlled liquid flow in a recycle
system by pumping.
Due to high efficiency, airlift bioreactors are
sometimes preferred e.g., methanol production,
waste water treatment, single-cell protein
production
24. There are two different process of
fermentation viz.:
(1) Batch fermentation
(2) Continuous culture.
Batch fermentation:
Nutrients are added in the fermentation for
the single time only and growth continues
until the particular nutrients are exhausted
25. In the batch process when the microorganism is
added into a medium which supports its growth,
the culture passes through number of stages
known as ‘growth curve’
A typical growth curve consists of following stages
a) Lag phase
b) Acceleration phase
c) Log or exponential phase
d) Deceleration phase
e) Stationary phase
f) Death phase
26. (a) Lag phase:
Immediately after inoculation, there is no increase
in the numbers of the microbial cells for some time
and this period is called lag phase. In this is phase
the organisms adjust to the new environment in
which it is inoculated into.
(b) Acceleration phase:
The period when the cells just start increasing in
numbers is known as acceleration phase.
(c) Log phase:
This is the time period when the cell numbers
steadily increase.
(d) Deceleration phase:
The duration when the steady growth declines.
27. (e) Stationary phase:
The period where there is no change in the
microbial cell number is the stationary phase. This
phase is attained due to depletion of carbon source
or accumulation of the end products.
(f) Death phase:
The period in which the cell numbers decrease
steadily is the death phase. This is due to death of
the cells because of cessation of metabolic activity
and depletion of energy resources.
Depending upon the product required the different
phases of the cell growth are maintained. For
microbial mass the log phase is preferred. For
production of secondary metabolites i.e. antibiotics,
the stationary phase is preferred.
28. GROWTH KINETICS OF BATCH
CULTURE
The number of living cells (population of growth rate
dN/dt)varies with time in a batch system as shown
below:
29. where;
LAG PHASE:
NUMBER OF BACTERIA DOES NOT CHANGE WITH TIME IN
LAG PHASE.
LOG Phase:
Number of bacteria increases exponentially in log phase.
30. DURING LOG PHASE THE
NUMBER OF ORGANISMS IN
THE REACTOR AT ANY
TIME T CAN BE
CALCULATED, BY USING
RATE EQUATION SHOWNbelow:
31. According to last equation, number of bacteria in the
reactor at any time t during log phase can be calculated,
as it is seen in the graph.
THIS RATE EQUATION CAN BE
INTEGRATED:
32. STATIONARY PHASE:
There is no net change in number of bacteria with time
in stationary phase. Bacteria divide but also die at
equal rate. Most of the important biological products
(especially secondary metabolites like antibiotics) or
biomass are produced during this phase.
The biomass concentration at stationary phase is
determined by following equation
X = Y. SR
X=cell concentration
Y= yield factor for limiting nutrient
SR = original nutrient concentration in the medium
33. Y’ measures the efficiency of a cell in
converting nutrients into biomass
So the biomass at a particular time in the
during the fermentation is given by the
following equation.
X = Y (SR -s)
S= nutrient concentration at particular time
thus ‘Y’ is represented by the following equation
Y = X/ (SR - s)
34. CONTINUOUS OPERATIONS
Continuous fermentation:
The growth rate and physiological conditions of
microorganisms can be maintained by using a
process of continuous culture (chemostat )
In this the products are removed continuously
along with the cells and the same is
replenished with the cell girth and addition of
fresh culture media. This results in a steady or
constant volume of the contents of the
fermenter. This type of fermentation is used for
the production of single cell protein (S.S.P),
antibiotics and organic solvents.
35. CONTINUOUS FERMENTATION
PROCESS
The dilution rate is the ratio of inflowing
amount of medium to the volume of the
culture.
Thus
D = F / V
D= dilution rate
F= Flow rate
V=Volume
36. The change in cell concentration of cells at
perticular time period is expressed by the
following equation
dx/dt= growth rate – output
Or dx/dt = μx - Dx
In the process of continuous culture technique
the output is balanced by growth hence,
μx = Dx
μ – D
Dx / dt= D
37. The biomass concentration in the
chemostat is determined by the
following equation
X = Y(SR - s)
X= steady state concentration
S= steady state residual concentration in
the medium
38. ADVANTAGES AND DISADVANTAGES OF BATCH AND
CONTINUOUS OPERATIONS
BATCH SYSTEMS
easy to operate and control
genetic stability of organism
could be controlled if it is
genetically engineered
biocatalyst.
lower contamination risk
non-productive down time is a
disadvantage
batch to batch variability is
problem
accumulation of inhibitory
products is problem
CONTINUOUS SYSTEMS
degeneration of
biocatalyst
higher contamination risk
is a disadvantage
efficient, higher
productivity
product is obtained with
uniform characteristics;
quality of the product is
almost same from time to
time
no accumulation of
inhibitory products.