This document describes an evaluation of the disinfectant activity of some commercial preparations using the Rideal-Walker test. Three disinfectants (Dettol, Lyzol, and Domex) were tested at manufacturer recommended concentrations on surfaces contaminated with various bacterial and fungal strains. The reduction in microbial counts before and after disinfection was measured as the log reduction. Phenol coefficients were calculated based on the dilution at which the disinfectant killed microbes within 7.5 minutes but not 5 minutes, relative to phenol. Dettol, Lyzol and Domex had average phenol coefficients of 1.315, 1.368 and 1.135 respectively, indicating their relative disinfecting
Short review about parenteral suspension, principle consideration during formulation, factors affecting formulation etc. are included in this presentation.
Evaluation of the efficiency of sterilization methods.Sterility indicatorsMs. Pooja Bhandare
Evaluation of the efficiency of sterilization methods.Sterility indicators
Sterility criteria: Bioburden ,Sensitivity of microorganisms
Death rate or Survivor curve,D- Value or Decimal reduction time,Z- value or Thermal reduction time, f- value, Q10 Value or Temperature Coefficient, Inactivation Factor:
STERILITY INDICATORS : Physical Indicators, Chemical Indicators
Biological Indicators
1. Physical Indicators: i) Moist heat Indicator ii) Dry heat iii) Radio sterilization iv) Gaseous methods v) Filtration 2.CHEMICAL INDICATORS : I) Browne’s tubes II) WITTNESS TUBES IV) Royce Sachet V) Chemical Dosimeter 3.BIOLOGICAL INDICATORS
Killing or removing all forms of microbial life (including endospores) in a material or an object.
Mainly due to: oxidation of cell component, denature proteins, nucleic acids, RNA and loss of membrane permeability.
Procedures performed in a way to prevent contamination with infectious microorganisms
Used to prevent contamination of surgical instruments, medical personnel, and the patient during surgery
Sanitization: Lowering of microbial counts to prevent transmission in public setting (e.g., restaurants & public rest rooms)
Degerming: Mechanical removal of microbes from limited area. e.g., Alcohol swab on skin, washing of hands with soap
Sepsis: Bacterial contamination
Antisepsis: Reduction or Inhibition of microbes found on LIVING TISSUE
Two general methods are used for microbiological assays
Method A: Cylinder plate method or cup plate method.
Method B: Tube assay method or titrimetric method.
Short review about parenteral suspension, principle consideration during formulation, factors affecting formulation etc. are included in this presentation.
Evaluation of the efficiency of sterilization methods.Sterility indicatorsMs. Pooja Bhandare
Evaluation of the efficiency of sterilization methods.Sterility indicators
Sterility criteria: Bioburden ,Sensitivity of microorganisms
Death rate or Survivor curve,D- Value or Decimal reduction time,Z- value or Thermal reduction time, f- value, Q10 Value or Temperature Coefficient, Inactivation Factor:
STERILITY INDICATORS : Physical Indicators, Chemical Indicators
Biological Indicators
1. Physical Indicators: i) Moist heat Indicator ii) Dry heat iii) Radio sterilization iv) Gaseous methods v) Filtration 2.CHEMICAL INDICATORS : I) Browne’s tubes II) WITTNESS TUBES IV) Royce Sachet V) Chemical Dosimeter 3.BIOLOGICAL INDICATORS
Killing or removing all forms of microbial life (including endospores) in a material or an object.
Mainly due to: oxidation of cell component, denature proteins, nucleic acids, RNA and loss of membrane permeability.
Procedures performed in a way to prevent contamination with infectious microorganisms
Used to prevent contamination of surgical instruments, medical personnel, and the patient during surgery
Sanitization: Lowering of microbial counts to prevent transmission in public setting (e.g., restaurants & public rest rooms)
Degerming: Mechanical removal of microbes from limited area. e.g., Alcohol swab on skin, washing of hands with soap
Sepsis: Bacterial contamination
Antisepsis: Reduction or Inhibition of microbes found on LIVING TISSUE
Two general methods are used for microbiological assays
Method A: Cylinder plate method or cup plate method.
Method B: Tube assay method or titrimetric method.
content-
Glass
Properties of glass
Raw materials
Composition of glass
Manufacture of glass
Advantages
Disadvantages
Type of glass
Quality control tests for glasses
Disinfectant - Pharmaceutical microbiology (Second year b.pharm) (3rd semester)Kiran Shinde
Prof.Mr.Kiran K. Shinde (M.Pharm), Assistant professor (VNIPRC)
Pharmaceutical microbiology (Second year b.pharm) (3rd semester)
Introduction
Classification & mode of action of disinfectant
Factors affecting disinfectant, antiseptics & their evaluation
Evaluation of bacteriostatic & bactericidal
Qc test for plastics,metallic tins,closures, collapsible tubes, secondary pac...himanshu kamboj
b pharma 6th sem
pharmaceutical quality assurance
Introduction
Types of pharmaceutical packaging
Packaging materials
Quality control test for plastic
Quality control test for closures
Quality control of collapsible tubes
Quality control of metallic tins
QC test for secondary packaging materials
Evaluation of Bactericidal and BacteriostaticRajsingh467604
What are disinfectants?
As per the definition given by WHO ( World health organization ) : a disinfectant is a chemical agent, which destroys or inhibits growth of pathogenic microorganisms in the non-sporing or vegetative state.
Why Evaluation?
Evaluation of disinfectants is used to check the ability or efficacy of any disinfectant against specific microorganisms to establish its effectiveness.
Evaluation tests of bactericide.
1. RIDEAL WALKER TEST
This test is also known as the phenol coefficient test,in which any chemical is compared with phenol for its antimicrobial activity.
The result is shown in the form of phenol coefficient.
▪ If a phenol coefficient of a given test disinfectant is less than 1, it means that disinfectant is less effective than phenol.
▪ If a phenol coefficient of a given test disinfectant is more than 1, it means that disinfectant is more effective than phenol.
Procedure
1.1 Different dilutions of the test disinfectant and phenol are prepared and 5 ml of each dilution is inoculated with 0.5ml of the 24 hour growth culture of the organisms.
1.2 All tubes(Disinfectant + organisms & phenol + organisms) are placed in a water bath ( at 17.5° C)
1.3 Subcultures of each reaction mixture are taken and transferred to 5ml sterile broth at an interval of 2.5 minutes from zero to 10 mintues.
1.4 Broth tubes are incubated at 37° C for 2 to 3 days & examined for the presence or absence of the growth.
1.5 Then the Rideal Walker coefficient is calculated :
2. CHICK MARTIN TEST.
CHICK MARTIN test is performed in the much similar way as the RIDEAL Walker test but with a little variation.
Principle : This test is carried out in the presence of organic matter like 3% human feces or dried yeast.
Procedure
2.1 Serial dilutions of test solution and phenol is prepared in distilled water.
2.2 To this 3% yeast suspension is also added.
2.3 To this solution the S. typhi is added
2.4 After contact time of 30 mins the above mixture is transferred to the freshly prepared 10 ml of broth.
2.5 The test tubes are incubated at 37°C for 48 hours.
2.6 Presence or absence of the growth is calculated.
Evaluation tests of Bacteriostatic.
1. Tube dilution & Agar plate Method
1.1 The chemical agent is incorporated into nutrient broth or agar medium and inoculated with test micro-organisms.
1.2 These tubes are incubated at 30° TO 35°C for 2 to 3 days and then the results in the form of turbidity or colonies are observed.
1.3 The results are recorded and the activity of the given disinfectant is compared.
2. Cup plate method
2.1 Agar is melted and cooled at 45° Celsius.
2.2 Then inoculated with test micro-organisms and poured into a sterile petri plate.
2.3 In the cup plate method, when the inoculated agar has solidified, holes around 8mm in diameter are cut in the medium with a steel cork borer.
2.4 Now the antimicrobial agents are directly placed in the holes.
Hello,
This part is an important one that describes the individual packaging material types & detailed discussion on their possible analysis before release from the QC end in the pharmaceutical industries.
Thanks for watching
Presentation showing various methods used for confirmation of sterilization processes. This includes various methods used for confirmation of sterilization done by filtration sterilization, Thermal sterilization, radiation sterilization, gaseous sterilization etc.
Sterility Testing is defined as a testing which confirms that products are free from the presence of viable microorganisms. Sterility testing is very important for medical devices, pharmaceuticals, preparations, tissue materials and other materials that claim to be sterile or free from viable microorganisms.
TESTING OF DISINFECTANT CLASSES OF DISINFECTANTS METHOD FOR TESTING DISINFEC...VeerendraMaravi
HISTORY
INTRODUCTION
CLASSES OF DISINFECTANTS
METHOD FOR TESTING DISINFECTANTS
CARRIER TEST
CAPACITY TEST
SUSPENSION TESTS
PRACTICAL TEST
IN USE TEST
Testing schemes
TEST ORGANISMS
content-
Glass
Properties of glass
Raw materials
Composition of glass
Manufacture of glass
Advantages
Disadvantages
Type of glass
Quality control tests for glasses
Disinfectant - Pharmaceutical microbiology (Second year b.pharm) (3rd semester)Kiran Shinde
Prof.Mr.Kiran K. Shinde (M.Pharm), Assistant professor (VNIPRC)
Pharmaceutical microbiology (Second year b.pharm) (3rd semester)
Introduction
Classification & mode of action of disinfectant
Factors affecting disinfectant, antiseptics & their evaluation
Evaluation of bacteriostatic & bactericidal
Qc test for plastics,metallic tins,closures, collapsible tubes, secondary pac...himanshu kamboj
b pharma 6th sem
pharmaceutical quality assurance
Introduction
Types of pharmaceutical packaging
Packaging materials
Quality control test for plastic
Quality control test for closures
Quality control of collapsible tubes
Quality control of metallic tins
QC test for secondary packaging materials
Evaluation of Bactericidal and BacteriostaticRajsingh467604
What are disinfectants?
As per the definition given by WHO ( World health organization ) : a disinfectant is a chemical agent, which destroys or inhibits growth of pathogenic microorganisms in the non-sporing or vegetative state.
Why Evaluation?
Evaluation of disinfectants is used to check the ability or efficacy of any disinfectant against specific microorganisms to establish its effectiveness.
Evaluation tests of bactericide.
1. RIDEAL WALKER TEST
This test is also known as the phenol coefficient test,in which any chemical is compared with phenol for its antimicrobial activity.
The result is shown in the form of phenol coefficient.
▪ If a phenol coefficient of a given test disinfectant is less than 1, it means that disinfectant is less effective than phenol.
▪ If a phenol coefficient of a given test disinfectant is more than 1, it means that disinfectant is more effective than phenol.
Procedure
1.1 Different dilutions of the test disinfectant and phenol are prepared and 5 ml of each dilution is inoculated with 0.5ml of the 24 hour growth culture of the organisms.
1.2 All tubes(Disinfectant + organisms & phenol + organisms) are placed in a water bath ( at 17.5° C)
1.3 Subcultures of each reaction mixture are taken and transferred to 5ml sterile broth at an interval of 2.5 minutes from zero to 10 mintues.
1.4 Broth tubes are incubated at 37° C for 2 to 3 days & examined for the presence or absence of the growth.
1.5 Then the Rideal Walker coefficient is calculated :
2. CHICK MARTIN TEST.
CHICK MARTIN test is performed in the much similar way as the RIDEAL Walker test but with a little variation.
Principle : This test is carried out in the presence of organic matter like 3% human feces or dried yeast.
Procedure
2.1 Serial dilutions of test solution and phenol is prepared in distilled water.
2.2 To this 3% yeast suspension is also added.
2.3 To this solution the S. typhi is added
2.4 After contact time of 30 mins the above mixture is transferred to the freshly prepared 10 ml of broth.
2.5 The test tubes are incubated at 37°C for 48 hours.
2.6 Presence or absence of the growth is calculated.
Evaluation tests of Bacteriostatic.
1. Tube dilution & Agar plate Method
1.1 The chemical agent is incorporated into nutrient broth or agar medium and inoculated with test micro-organisms.
1.2 These tubes are incubated at 30° TO 35°C for 2 to 3 days and then the results in the form of turbidity or colonies are observed.
1.3 The results are recorded and the activity of the given disinfectant is compared.
2. Cup plate method
2.1 Agar is melted and cooled at 45° Celsius.
2.2 Then inoculated with test micro-organisms and poured into a sterile petri plate.
2.3 In the cup plate method, when the inoculated agar has solidified, holes around 8mm in diameter are cut in the medium with a steel cork borer.
2.4 Now the antimicrobial agents are directly placed in the holes.
Hello,
This part is an important one that describes the individual packaging material types & detailed discussion on their possible analysis before release from the QC end in the pharmaceutical industries.
Thanks for watching
Presentation showing various methods used for confirmation of sterilization processes. This includes various methods used for confirmation of sterilization done by filtration sterilization, Thermal sterilization, radiation sterilization, gaseous sterilization etc.
Sterility Testing is defined as a testing which confirms that products are free from the presence of viable microorganisms. Sterility testing is very important for medical devices, pharmaceuticals, preparations, tissue materials and other materials that claim to be sterile or free from viable microorganisms.
TESTING OF DISINFECTANT CLASSES OF DISINFECTANTS METHOD FOR TESTING DISINFEC...VeerendraMaravi
HISTORY
INTRODUCTION
CLASSES OF DISINFECTANTS
METHOD FOR TESTING DISINFECTANTS
CARRIER TEST
CAPACITY TEST
SUSPENSION TESTS
PRACTICAL TEST
IN USE TEST
Testing schemes
TEST ORGANISMS
TLC for chlorinated pesticide determination Bapi Mondal Bapi Mondal
In this assignment file i will demonstrate the process involved in Thin layer chromatography for Chlorinated Pesticide
determination. if u like this work feel free to share this file. thank you.
Anti-Adhesion and Anti-Biofilm Effectiveness of Disinfectants Used In Hemodia...IJERA Editor
Biofilms are communities of microorganisms attached to a surface and included in an extracellular matrix making it resistant to exogenous deleterious agents. The aim of this study is to evaluate the anti-adhesive and anti-biofilm effect of five commercials disinfectants having different active principles (hydrogen peroxide, sodium hypochlorite, isopropyl alcohol and ethanol) on four Staphylococcus strains isolated from hemodialysis unit surfaces. The disinfectants anti-adhesive effect was estimated to an exceeding rate 70% for the various studied dilutions and 90% towards the pure products. Whereas the anti-biofilm effect showed an elimination rate varying between 10 % and 95 % according to the following parameters: active principle, time of contact, concentration and bacterial strain. Our study demonstrated that all tested products have an interesting anti-adhesive effect and that the peroxide of hydrogen is endowed with important anti-biofilm efficiency, followed by the alcoholic products and the sodium hypochlorite.
IJRET : International Journal of Research in Engineering and Technology is an international peer reviewed, online journal published by eSAT Publishing House for the enhancement of research in various disciplines of Engineering and Technology. The aim and scope of the journal is to provide an academic medium and an important reference for the advancement and dissemination of research results that support high-level learning, teaching and research in the fields of Engineering and Technology. We bring together Scientists, Academician, Field Engineers, Scholars and Students of related fields of Engineering and Technology
Phytochemical analysis, protein content & antimicrobial activities of sel...eSAT Journals
Abstract Two seed samples of Glycine max Linn. (S1, S2) were purchased from two retail stores of local market. Non-sprouted and sprouted seed powder were extracted separately with methanol (100%, 50%) by cold maceration to obtain methanolic and hydroalcoholic extract of Glycine max Sample 1 was designated as MES1 and HES1 and sample 2 as MES2 and HES2 respectively. Phytochemical analysis indicated the presence of various phytoconstituents viz. phytosterols, flavonoids, phenolic compounds, tannins, carbohydrates, proteins, amino acids, fixed oils and fats etc. Thin layer chromatography study on extracts revealed the presence of a number of compounds. The protein content of these samples were studied. The protein content of samples MES1, HES1, MES2 and HES2 with respect to BSA was found to be 90.6 2μg/ml, 82μg/ml, 94.5μg/ml and 79.1μg/ml respectively. The highest among these were found to be in MES2. Sprouting enhanced the protein content of the two samples. The samples have shown antimicrobial activity at selected concentration and microbial strains (26mm) for gram negative bacteria (27mm) for gram positive bacteria. Keywords: Glycine max Linn, phytochemical constituents, TLC, antimicrobial activity, protein, methanolic extract, hydroalcoholic extract.
Synthesis, Characterization and Biological Evaluation of Substituted Thiazoli...paperpublications3
Abstract: A new series of substituted thiazolidin-4-ones were synthesized and evaluated for antimicrobial activity by means of zone of inhibition by cup plate method. The structures of these compounds were established by means by IR, H NMR analysis. All the compounds were evaluated for their antibacterial activity against gram positive and gram negative species and antifungal activity .Compounds TH09 & TH17 were found most active due to presence of electron withdrawing groups at appropriate position.
Patient compliance: Challenges in management of cardiac diseases in Kuala Lum...pharmaindexing
Background
The objective of this study was to investigate the degree of compliance among cardiac patients who attend the health facilities in Kuala Lumpur and Perak, Malaysia. The reasons for non-compliance and recommendations from healthcare professionals were also evaluated.
Method
A cross-sectional study of 400 patients and 100 healthcare professionals was carried out. This study utilizes variables on external factors and internal factors as the measurement tools. The questionnaire which consists of Morisky self-reported medication adherence questions was administered to patients and causes for non-compliance sought. Questionnaire for healthcare professionals was used to determine strategies that can improve compliance rate.
Results
The study revealed a 15.8% of high adherence rate, 54.3% of moderate adherence rate and 30% of poor adherence to cardiovascular disease medications. The chi-square tests showed the strong association between dependent and independent variables. The model chosen for testing the patient compliance through external and internal factors gives an R2 value of 85.0% with an adjusted R2 of 84.7%. The F value (317.187) was also significant (p=0.000) which means that the variables have better fit in the multivariate model. The major reasons determined for non-adherence were attitudes and beliefs, lifestyle, side effects and cost of medications. The study recommends that pharmacists and dispensing technicians should be adequately qualified to provide proper counselling to cardiac patients on their medicines and disease conditions.
Conclusion
The result of this study is of value to health care providers. Compliance to cardiovascular medications will avoid treatment failures encountered in therapy.
Overview on Recurrence Pregnancy Loss etiology and risk factorspharmaindexing
Recurrent pregnancy loss (RPL) can be defined as more than two to three consecutive miscarriages before 20 weeks’ gestation; it affects approximately 1% to 2% of women. RPL is a multifactorial disease. It is very important to study the etiology and risk factors of RPL to find the best diagnostic tests and suitable therapeutic intervention. This article will discuss the current understanding etiologies and risk factors of RPL.
Novel treatments for asthma: Corticosteroids and other anti-inflammatory agents.pharmaindexing
Asthma management is a challenge due to the prevalence of disease in the world. Based on the immunological and inflammatory mechanisms of asthma, corticosteroids and anti-inflammatory participate greatly in the treatment plan. Due to different reasons, there is still an unmet need to develop new agents in this field. A lot of compounds with anti-inflammatory effect are investigated in both pre-clinical and clinical studies.
A review on liver disorders and screening models of hepatoprotective agentspharmaindexing
The liver is a vital organ present in vertebrates and some other animals. It has a wide range of functions, including detoxification, protein synthesis, and production of bio chemicals necessary for digestion. The liver is necessary for survival; there is currently no way to compensate for the absence of liver function long term, although liver dialysis can be used short term.
Carbamazepine induced Steven Johnson syndrome: A case reportpharmaindexing
Drugs are the most common cause that induces Steven Johnson syndrome (SJS) and includes antiepileptic drugs, antiretroviral drugs, anti-tuberculosis drugs, Sulphonamides, fluoroquinolones, penicillins, non-Steroidal anti-inflammatory drugs, Multivitamins. The genetic markers are also the cause for carbamazepine induced Steven Johnson Syndrome. In our study, the antiepileptic drug (Carbamazepine) is the cause for Steven Johnson Syndrome. A female patient aged 25 years came to the hospital with the complaints of bubbling over the skin and all over the body with papillary vesicles associated with pain and irritation, fever, myalgia, and nausea. The patient is known case of Phenytoin induced Steven Johnson Syndrome. In this case the patient developed the Steven Johnson Syndrome approximately after one month after starting the carbamazepine.By the withdrawal of the drug, the condition of the patient was improved.
Monoherbal formulation development for laxative activitypharmaindexing
The Ayurvedic Pharmacopoeia specifically approves flaxseed as a poultice for boils externally and demulcent or laxative internally. In this study monoherbal formulation development for laxative activity of flaxseed was undertaken. The plantLinumusitatissimumhasshowed higher percentage of total ash as well as alcohol soluble extractive values. The aqueous extract of Linumusitatissimumwas prepared by using pilot scale extraction plant and spray drying unit. The qualitative phytochemical studies reveal the presence of amino acids, carbohydrates, vitamins and proteins. From the available literatures it was found that Linumusitatissimum contains more number of amino acids. The formulated tablets showed acceptable pharmacopoeial limits and complies with specifications for thickness, hardness, friability and weight variation. The formulation has showed better laxative activity indicating additive property of the combined phytoconstituents of the plant.
Monoherbal formulation development for laxative activitypharmaindexing
The Ayurvedic Pharmacopoeia specifically approves flaxseed as a poultice for boils externally and demulcent or laxative internally. In this study monoherbal formulation development for laxative activity of flaxseed was undertaken. The plantLinumusitatissimumhasshowed higher percentage of total ash as well as alcohol soluble extractive values. The aqueous extract of Linumusitatissimumwas prepared by using pilot scale extraction plant and spray drying unit. The qualitative phytochemical studies reveal the presence of amino acids, carbohydrates, vitamins and proteins. From the available literatures it was found that Linumusitatissimum contains more number of amino acids. The formulated tablets showed acceptable pharmacopoeial limits and complies with specifications for thickness, hardness, friability and weight variation. The formulation has showed better laxative activity indicating additive property of the combined phytoconstituents of the plant.
Pneumonia and respiratory failure from swine origin influenza H1n1pharmaindexing
Swine influenza (swine flu) became alarming health concern when World Health Organization declared as “public health emergency of international concern” on April 25, 2009. After documentation of human-to-human transmission of the virus in at least three countries of two WHO regions, the WHO raised the pandemic level to 6.1 During the 1918, flu pandemic infected one-third of the world's population (an estimated 500 million people) and caused approximately 50 million deaths.2 In 1976, an outbreak of swine influenza occurred in New Jersey, USA, which involved more than 200 cases, some of them severe, resulting in one death.3 In 1988, another fatality was reported as a complication of swine influenza.
A descriptive study on newborn care among postnatal mothers in selected mater...pharmaindexing
The newborn health challenge faced by India is more formidable than that experienced by any other country in the world. The newborn health is inevitably affected by the traditional care practices of the mothers causing high infant morbidity and mortality.The aim of the study were determine the knowledge, attitude and practice of postnatal mothers regarding new born care and find out the association between knowledge, attitude and practice of postnatal mothers regarding new born care and to determine the association between these as well as with the selected demographic variables. A descriptive study was conducted to assess the knowledge, attitude and practice of postnatal mothers regarding new born care in selected maternity centres in Madurai. Survey approach was employed to select sample and it consisted of 100 postnatal mothers. Data was collected using structured interview schedule. Findings of the study showed that 65% of postnatal mothers had moderate knowledge; 61% had favourable attitude and 57% of them had high practice of new born care. There was a significant association between knowledge and attitude (r=+0.567), knowledge and practice (r=+0.388), attitude and practice (r=+0.321) .There was a significant association between knowledge and education, monthly family income and obstetrical score at p<0.05. Findings of the study indicated the need to conduct frequent assessment of knowledge, attitude and practice of postnatal mothers regarding new born care. Awareness and attitude of the mothers towards new born care still has lots of lacunae especially in those who belong to the lower socio economic statusand poorly educated postnatal mothers. So it is imperative to provide comprehensive training in the field of new born care for mothers during pregnancy
Late 19th century was evident of intelligent biomaterial; which has changed researcher’s perspective towards science and technology. This intelligent biomaterial are envisioned to have huge impact on Healthcare from sequential signalling of biomedical molecule, mimicking natural gene, an effective drug carrier, to high resolution diagnostic tool.From drug discovery aspect many of NCE fail to reach therapeutic potential due to PK/ PD profile. Nanotechnology has changed the face of drug discovery form chemical evaluation to structure of proteins in signalling pathways and development of chemical antibody. Nanotechnology from lab to market approval is long process due to regulatory evaluation. Though it seems to be bright future market it has to go through a long process from being innovation to complete market product. This makes whole process expensive making investor reluctant to invest in big projects.Western world is aware of dramatic potential of nano-projects; which has its limitation in financial investments; with major challenge of transforming nano science to commercial pharmaceutical product.
The Flaws in health practice in post-operative management of a patient in ter...pharmaindexing
Introduction
Congenital urinary tract obstructions are common cause of kidney damage sometimes which sometimes presents itself without symptoms leading to abnormalities in blood filtration and consequently retarded kidney function. A cohort study was conducted in such patient to find out the short comings in treatment strategy.
Case presentation
A four years old child, weighing 14 kg was brought with severe constipation, fever, chest congestion and cough later developed left eye disorientation after admission to hospital, diagnosed with urinary tract obstruction, indicating acidosis and loss of electrolytes due to excessive loss of water. His therapy management included surgical treatment, dialysis and to improve his electrolyte levels within the normal with the treatment chest congestion and fever.
Conclusion
This case study reports the post operative treatment of congenital urinary tract obstructions in a tertiary care hospital and highlights the discrepancies observed. Antibiotic rationality and irrational prescribing was observed. The case study highlights the need of a clinical pharmacist in the health care team.
Corticosteroid induced disorders – An overviewpharmaindexing
Glucocorticoids are important in the treatment of many inflammatory, allergic, immunologic, and malignant disorders, and the toxicity of glucocorticoids is one of the commonest causes of iatrogenic illness associated with chronic inflammatory disease.Glucocorticoid-induced muscle atrophy is characterized by fast-twitch or type II muscle fiber atrophy. Corticosteroid (CS) therapy is widely used in the treatment of rheumatic diseases.Osteoporosis remains one of its major complications.Steroid induced glaucoma is a form of open angle glaucoma occurring as an adverse effect of corticosteroid therapy. Glucocorticoids induce hepatic and extrahepatic insulin resistance.Glucocorticoid treatment impairs both glucose transport in fat and muscle cells. Corticosteroid-induced psychosis represents a spectrum of psychological changes that can occur at any time during treatment. Cushing’s syndrome describes the signs and symptoms associated with prolonged exposure to inappropriately high levels of the hormone cortisol. Physicians must be aware of these adverse effects and be equipped to manage them.
Anti-inflammatory activity of pupalia lappacea L. Jusspharmaindexing
Pupalia lappacea (L) Juss is an erect shrub used in folklore medicine to treat bone fractures and in inflammatory conditions. Methanolic extract of aerial parts shown is claimed in traditional medicine that the leaves of the plant are used in the treatment of inflammation. In the present study, the methanolic extract of Pupalia lappacea was screened for its anti-inflammatory activity using carageenan induced rat paw edema egg white induced paw oedema models. The methanolic extract at the dose of 200 mg/kg p.o exhibited significant anti-inflammatory activity in carrageenan induced paw edema model (p<0.01). In egg white induced model, methanolic extract at the dose of 200 mg/kg inhibited paw oedema significantly (p<0.01) indicating that both test samples inhibit the increase in number of fibroblasts and synthesis of collagen and mucopolysaccharides during prostaglandin formation during the inflammation. These experimental results have established a pharmacological evidence for the folklore claim of the drug to be used as an anti inflammatory agent. HPTLC analysis of the extract shows the presence of gallic acid 1.24mg/ml, ferulic acid 2.00mg/ml, chlorogenic acid 46.25mg/ml and rutin 7.02mg/ml of the extract which were responsible for the claimed anti-inflammatory action in the animal models studied.
Lucinactant: A new solution in treating neonatal respiratory distress syndrom...pharmaindexing
Lucinactant is a novel synthetic surfactant, approved by the FDA on March 6th 2012, for use in treatment of RDS. It’s superiority as compared to the previously approved surfactants lie in containing sinapultide, a 21-amino acid peptide also known as KL4 peptide, which has been designed to mimic the activity of human surfactant protein. Lucinactant is completely devoid of any animal derived components. It is the fifth drug approved by the FDA for the treatment of RDS. It has shown immense efficacy in phase two clinical trials and animal model studies and exhibited better efficiency when compared to other surfactants in both 24 hour and two week mortality rates of infants in RDS. Lucinactant tends reduce the surface tension at the air-liquid interface of alveolar surfaces and allows lungs to function normally. It was observed that the side effects were lesser with Lucinactant when compared with other naturally derived surfactants.
Bioactivity screening of Soil bacteria against human pathogenspharmaindexing
Microorganisms have a profound effect on medical science as they not only infect & cause disease but also produce metabolic products that can cure infections. Soil happens to be a source for a variety of microorganisms. Most of the bacteria, particularly actinomycetes produce biologically active secondary metabolites. Though there are a number of antibiotics available, there is a pressing need for the discovery of new source for antimicrobials against the pathogens due to the development of drug resistance of the pathogenic microorganisms. In addition to, new pathogenic strains are also developing and causing infection to human beings. Bioactive compounds are compounds that are produced by any living organism and are known to exhibit various biological activities both in-vitro & in-vivo. Bioactivity may be antimicrobial, antineoplastic, anticancerous, immunomodulation, antifertility & others. Soil bacteria were isolated by standard technique and by making use of selective media. The isolates were identified and subjected for preliminary screening to look for their ability to produce bioactive materials. A total of 96 strains were isolated from three different soil samples. 14 of them were found to have antibacterial activity against the human pathogens like Staphylococcus aureus, Streptococcus faecalis, E.coli, Klebsiella aerogenes, Proteus vulgaris, Pseudomonas aureginosa and Salmonella typhi by preliminary screening. Further the selected (3) bacteria were grown in the suitable culture media for the production of bioactive metabolites by using rotary shake flask. The active metabolites was isolated by solvent extraction and concentrated by evaporation under reduced pressure. The antimicrobial screening of the active metabolites showed prominent effect against the clinical pathogens under the study.
A study on sigmoid Volvulus presentation and managementpharmaindexing
A study on sigmoid volvulus presentation and management was a 2yr retrospective study done at RMMCH.The diagnosis of sigmoid volvulus was made from a history of large bowel obstruction (constipation, abdominal distension, and abdominal pain), which were often recurrent and plain abdominal radiographs.The morbidity associated isSuperficial wound infection occurred in four patients. All the infected wounds eventually healed with conservative measures. Clinical anastomotic dehiscence was noted in 1 patient for which during relaparotomy proximal colostomy and mucous fistula was done. The mortality associated is shown is there were 9 deaths of which 7 were due to sepsis and 2 were due to comorbid illness. Two out of eight patients for whom a colopexy was done had a recurrent attack of sigmoid volvulus. The duration of hospital stay ranged between 10 and 21 days. Use of sigmoidoscopic detorsion for viable colon should be encouraged. Sigmoidopexy, which is associated with a recurrence rate of 20% in our series of patients, should be used selectively.Hartmann’s procedure is a safe option in sigmoid volvulus with gangrenous bowel. Primary anastomosis in emergency situation can be carried out with morbidity and mortality in patients with viable colon
Evaluation of Preliminary phytochemical on various some medicinal plantspharmaindexing
The present study was carried out to evaluate the physical status and percentage yield of methanolic extract and its fractions of whole plant of Leucas cephalotes, leaves of Hiptage benghalensis and leaves of Kydia calycina were recorded for future references and Preliminary phytochemical screening of MLC, MHB and MKC revealed the presence of carbohydrates, glycosides, saponins, flavonoids, steroidal and phenolic compounds. MLC revealed the presence of all the above mentioned phytoconstituents except saponins and also MKC steroidal compounds. The fractions of MLC, MHB and MKC revealed the presence of glycosides, phenolic compounds, steroids and flavonoids.
Comparision of in vitro antibacterial activity of cefoperazone and levofloxac...pharmaindexing
Cefoperazone (a third generation cephalosporin) has effective in vitro activity against majority of pathogens. Levofloxacin (a flouroquinolone) is one which prescribed more due to its increased antibacterial activity against Gram-positive, Gram-negative, and atypical bacteria. Microbial resistance to antibiotics is now prevalent and poses a serious clinical threat. An attempt has been made to evaluate sensitivity of Cefoperazone and Levofloxacin against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella typhi. A total of 120 isolates were collected from different pathological laboratories and medical centers in Karachi, Pakistan. The above stated clinical isolates were extracted from urine/stool, skin, blood and sputum samples. Results show least resistance of Levofloxacin as compare to Cefoperazone against Escherichia coli (32.5% and 42.5%) and Pseudomonas aeruginosa (36% and 48%) while Staphylococcus aureus is still susceptible towards Cefoperazone and least sensitive to Levofloxacin by showing 26.6% and 50% resistance respectively. Study concluded that the prevalent pathogens are still susceptible towards Levofloxacin and Cefoperazone but the gradual increase in resistance is alarming to the general practice of prescribing antibiotic which require routine evaluation and surveillance to ensure the effectiveness of the antibacterial agents.
Concept of srotas from ayurvedic perspective with special reference to neurologypharmaindexing
Ayurveda is a life science. The researchers of ayurveda could rule out the presence of srotas (channels) spreading throughout the human body. These srotas (channels) are governed by vayu which is using all the srotas (channels) of the body to carry out the functional and physiological activities of the human body without which the human society will not exist. Several synonymous words have been described by the ayurvedicacharyas for srotas. Some are micro and some are macro in structures and they adopt the same colour of the particular dhatus of the body to which it belongs. The aim of the study is to justify that srotas are nothing but innurmerable channels or pathways of the nervous system governed by electric current without which no functional and physiological activities of the human body will develope.
Health promotion survey in overweight and obese students of universities in n...pharmaindexing
Introduction
Overweight and obesity is one of the major health problems in the UK and worldwide. Approximately two-thirds of the population in the UK is either overweight or obese. Overweight and obesity is an important issue that causes distress to most women. Health promotion is the best method to educate overweight and obese women. It is defined as the process enabling people to increase control over and to improve their health by Ottawa Charter for Health Promotion. It is aimed to enhance the well-being of the individuals and their positive attitudes towards prevention of various diseases. In order to make any improvement to the health promotion for overweight and obesity, the risk factors and the opinions from the public should first be identified and addressed.
Methods
Cross-sectional survey design was selected with a questionnaire that consisted of 20 open and close ended questions. A sample size of 196 was determined. The data thus gathered was analyzed using SPSS V20 (Statistical Package for Social Science version 20). Descriptive statistics (fx) and (SD) were used and Chi-square X2 test for association was employed.
Results
Out of the total 196 responses, only (40%) of the students had normal weight (SD 1.1), (25%) students had a good understanding of health promotion (SD 1.6), half (50%) appeared concerned about their weight (SD 0.5), (60%) had an obese family member (0.5). The BMI of students was associated with the presence of an obese member in their family and their weight as a concern for them. (P-value <0.05).
Conclusion
The health promotion service is beneficial as it was found to have raised concerns in the mind of the students regarding over weight and obesity. However it was observed that the understanding of health promotion service was different among students and this is the root of the problem.
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Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
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Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
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NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
Prix Galien International 2024 Forum ProgramLevi Shapiro
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ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
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- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
Acute scrotum is a general term referring to an emergency condition affecting the contents or the wall of the scrotum.
There are a number of conditions that present acutely, predominantly with pain and/or swelling
A careful and detailed history and examination, and in some cases, investigations allow differentiation between these diagnoses. A prompt diagnosis is essential as the patient may require urgent surgical intervention
Testicular torsion refers to twisting of the spermatic cord, causing ischaemia of the testicle.
Testicular torsion results from inadequate fixation of the testis to the tunica vaginalis producing ischemia from reduced arterial inflow and venous outflow obstruction.
The prevalence of testicular torsion in adult patients hospitalized with acute scrotal pain is approximately 25 to 50 percent
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
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These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
Evaluate the Disinfectant Activity of Some Commercial Preparations by Rideal -Walker Test.
1. 48
* Corresponding author: B.Ram Sarath kumar.
E-mail address: ramsarathkumar@gmail.com
IJPAR |Volume 2 | Issue 2 | Apr - Jun- 2013 ISSN: 2320-2831
Available Online at: www.ijpar.com
[Research article]
Evaluate the Disinfectant Activity of Some Commercial Preparations by
Rideal -Walker Test.
*B.Ram Sarath kumar, Y.Ashok, R.Srinivasan, P.Radhika Reddy.
Siddhartha Institute of Pharmaceutical Sciences, Jonnalagadda, Narasaraopet,
Guntur (Dt), India.
ABSTRACT
The aim of this study was to evaluate and compare practically achieved disinfection efficacy of some locally
available disinfectants on surfaces and infectious microbiological hospital waste. Three disinfectants were tested
at concentrations recommended by manufacturers on rough and smooth surfaces that were contaminated
experimentally by locally circulating isolates of methicillin-resistant Staphylococcus aureus, multi drug-
resistant Acinetobacter baumannii, Klebsiella pneumoniae, Enterobacter aerogenes, Pseudomonas aeruginosa
strains, standard isolate of Salmonella typhi and Candida albicans. Reduction in microbial counts before and
after surface disinfection was expressed as log reduction. A very heavy microbial waste load was simulated by
immersing culture plates with heavy microbial growth in disinfectants. Daily, a sample of disinfectant was taken
and subjected to Rideal-walker test.
Keywords: Disinfectant, evaluation, hospital practice.
INTRODUCTION
Disinfection is the process of destruction or
removal of pathogenic microorganisms and the
object is said to be disinfected. The control of
micro organisms extends into many diverse areas
such as pharmaceuticals, medicine, hospital
environment, food processing, and every day
household. Whereas the destruction or removal of
all forms of life is called sterilization, disinfection
is the process of destruction or removal of
pathogenic micro organisms and the object is said
to be disinfected.
Ancient Egyptians used antibacterial oils, spices
and balsams as disinfectants. Chlorine compounds
were referred to as powerful disinfecting agents in
1827 in Lancet. Compounds including chlorine gas,
silver nitrate, phenol, zinc compounds and iodine
were used as disinfectants in the first half of the
19th
century (1, 2). Solution of chlorinated lime was
used for hand disinfection in Vienna in 1861.
Pasteur and Lister also contributed in the field;
ultimately Koch (1881) tested the action of
different disinfectants on pure cultures.
The term disinfection is generally used for a
process in which micro organisms present on non
living or inanimate objects and surfaces are killed
using chemical substances (3,10). The process does
not necessary free the surfaces from the bacterial
spores. The commonly used disinfectants belongs
to the categories namely phenol and its derivatives,
compounds of heavy metals, mercury compounds
organic chemicals, soaps, synthetic detergents and
alcohols (8, 9). The damage to the micro organisms
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occurs by denaturation or coagulation of cell
components. It may also occur by non specific
combination of the disinfectants with the cell
compounds like cell wall, proteins, nucleic acids
etc. The rate and extent of disinfectant action of
any substance generally depends on many factors
including, time of expose, temperature, pH,
concentration, surface tension, etc.
Based on their rate of reaction and potency
disinfectants have been arbitrarily classified in to 3
groups namely strong, week and moderate (4,5).
The effectiveness of disinfectants is highly
variable. An ideal disinfectant should be effective
against all type of microorganisms. In other words,
it should have a broad spectrum of activity at low
concentrations. The efficacy of disinfectants can be
determined by mixing them with known
microorganisms under controlled experimental
conditions and then inferring the extent of damage
to the microbial cultures from the observations
(11,12).
MATERIALS AND METHODS
The following materials were used in the present
study:
APPARATUS
Sterilized conical flask.
Test tubes
Sterile pipettes
Cotton plugs
Petri plates
Boiling tubes.
Measuring cylinder.
MATERIALS
Table 1: List of Chemicals used in present study
S.
No
Name of the
chemicals
Name of the company Batch number
1 Beef extract Finar chemical limited 19105950
2 Peptone Finar chemical limited 19105364
3 Sodium chloride Finar chemical limited n007j10
4 Distilled water Accent diagnostic center 14
EQUIPMENTS
Table 2: list of equipments used in the present study
S. No Name of the equipment Maker name
1 Hot air oven Bio-techniques India.
ISO 9001:2000
2 Incubator Kadavil electro mechanical(kemi)
3 Laminar air flow Kadavil electro mechanical(kemi)
4 Autoclave Kadavil electro mechanical(kemi)
5 Electronic weighing balance Dhona 200D
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Commercial disinfectants used in present study
Table 3: List of Commercial disinfectants used in present study
S. No Commercial Name
of chemical
disinfectants
Description
1 Domex Active ingredients: Bezolkonium chloride solution 2%w/v, non
ionic pine oil, parachoro meta cresol, water, perfume
Hindustan unilever limited
Mfg: 12/12
Exp: 18 months from mfg
Caution: keep out of reach of children
Storage: store upright in a cool place
2 Lyzol Active ingredients: Bezolkonium chloride i.p 4%w/v, tartrazine
yellow, preservative, perfume, water
Rickettbenckiser India limited
Mfg: 10/12
Exp: 09/13
Caution: do not mix with any other household cleaner or acid
Storage: store upright in a cool place
3 Dettol Active ingredients: Chloroxylenoli.p 4.8%w/v, terpenoli.p
9%w/v, alcohol absolute denature 13.1%v/v
Rickettbenckiser India limited
Mfg: 11/12
Exp: 4/15
Storage: store upright in a cool place
RIDEAL-WALKER METHOD
Procedure:
Dilutions of the test disinfectant and phenol are
first prepared and quantities each of 5ml are
measured of four chosen dilutions in each of sterile
tubes. Place the tubes alongside the 24 hours broth
culture in a rack in a water bath at 17.50 + 0.50;(1)
fifth tube contains 5ml of one of the standard
phenol dilutions (usually 1 in 105 dilutions). At 30
seconds intervals, the tubes are inoculated with 0.2
ml of the culture by means of a pipette and shake
the tubes gently after each inoculation(6). Thirty
seconds after the inoculation of the fifth tubes i.e.
2½ minutes after inoculating the first tube, remove
the first tube from the rack, shake it gently transfer
one standard loop full to a 5ml tube or R-W broth
and return the tube to the rack make sure that the
amount removed a loop full droplet and not just a
film within the loop)(7,8). The procedure was
Repeat 30 seconds intervals with each reaction tube
of the disinfectant in turn until the whole cycle has
been repeated four times, i.e. from each reaction
tube subcultures have been made after intervals of
2.5, 5. 7.5 And 10 minutes. Incubate the broth
tubes at 370
C for not less than 48 hours and not
more than 72 hours and record the presence of
absence growth in each tube.
Phenol coefficient = Dilution of disinfectant which kills in 7.5 mints but not in 5mints
Dilution of phenol which kills in 7.5 mints but not in 5mints.
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RESULTS
1) DETTOL
TRAIL 1.a
CONCENTRATION
TIME(MINS)
2.5 5 7.5 10
Standard phenol
1:95 + + _ _
Test Dilutions
1:95 _ _ _ -
1:105 _ _ _ _
1:115 + + + _
1:125 + + _ _
1:135 + _ _ _
Phenol coefficient = Dilution of disinfectant which kills in 7.5 but not in 5mins
Dilution of phenol which kills in 7.5 but not in 5mins
= 125÷95
= 1.315.
TRAIL 1.b
CONCENTRATION
TIME(MINS)
2.5 5 7.5 10
Standard phenol
1:95 + + _ _
Test Dilutions
1:95 _ _ _ _
1:105 _ _ _ _
1:115 + _ _ _
1:125 + + _ _
1:135 + + + _
Phenol coefficient = Dilution of disinfectant which kills in 7.5 but not in 5mins
Dilution of phenol which kills in 7.5 but not in 5mins
= 125÷95
= 1.315.
Average value of Dettol = 1.315+1.315/2
= 1.315.
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2) LYZOL
TRAIL 2.a
CONCENTRATION
TIME(MINS)
2.5 5 7.5 10
Standard phenol
1:95 + + __ _
Test Dilutions
1:95 _ _ _ _
1:105 + _ _ _
1:115 _ _ _ _
1:125 + + _ _
1:135 + + _ _
Phenol coefficient = Dilution of disinfectant which kills in 7.5 but not in 5mins
Dilution of phenol which kills in 7.5 but not in 5mins
= 125+135÷2
= 130
= 130÷95
= 1.368.
TRAIL 2.b
CONCENTRATION
TIME(MINS)
2.5 5 7.5 10
Standard phenol
1:95 + + _ _
Test Dilutions
1:95 _ _ _ _
1:105 _ _ _ _
1:115 _ _ _ _
1:125 + + _ _
1:135 + + _ _
Phenol coefficient = Dilution of disinfectant which kills in 7.5 but not in 5mins
Dilution of phenol which kills in 7.5 but not in 5mins
= 125+135÷2
= 130÷95
= 1.368.
Average value of lyzol = 1.368+1.368/2
=1.368.
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3) DOMEX:
TRAIL 3.a
CONCENTRATION
TIME(MINS)
2.5 5 7.5 10
Standard phenol
1:95 + + __ _
Test Dilutions
1:95 _ _ _ -
1:105 _ _ _ _
1:115 + _ _ _
1:125 + + _ _
1:135 + + + _
Phenol coefficient = Dilution of disinfectant which kills in 7.5 but not in 5mints
Dilution of phenol which kills in 7.5 but not in 5mints
= 125÷95
= 1.315.
TRAIL 3.b
CONCENTRATION
TIME(MINS)
2.5 5 7.5 10
Standard phenol
1:95 + + _ _
Test Dilutions
1:95 _ _ _ _
1:105 + + _ _
1:115 + _ _ _
1:125 + + + _
1:135 + + _ _
Phenol coefficient = Dilution of disinfectant which kills in 7.5 but not in 5mins
Dilution of phenol which kills in 7.5 but not in 5mins
= 135+105÷2
= 240÷95
= 1.263.
Average values of domex = 1.368+1.263/2 =1.135.
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DISCUSSION
In present investigation an attempt was made to
evaluate the disinfectant of commercial
preparations namely domex, Dettol, lyzol, white
phenol by following standard procedure employing
Staphylococcus aureus as test organism as
indicated in tables it was found that lyzol shows
good disinfectant activity among other the over
value phenol coefficient was found to be highest in
case of lyzol. As indicate in 2a, 2b &2c the phenol
coefficient of LYZOL was observed as 1.368. As
indicate in 3a, 3b &3c the phenol coefficient of
DOMEX was observed as 1.135. As indicate in 1a,
1b &1c the phenol coefficient of DETTOL was
observed as 1.332.
The order of disinfectant activity of the selected
chemical disinfectants is as follows:
LYZOL > DOMEX > DETTOL.
CONCLUSION
The present investigations of 3 different
commercial chemical disinfectants were evaluated
for their disinfectant actually by following standard
procedure.Among 3 different commercial chemical
preparations Lysol has sharan good disinfectant
activity against the test organism Staphylococcus
aureus.
The older of disinfectant activity of the selected
chemical disinfectants is as follows:
LYZOL > DOMEX > DETTOL.
REFERENCE
[1] Ananthanarayana and panikers, Text book of microbiology 11th
edition. Page .no 25-33.
[2] Pelczar/krieg microbiology, 5th
edition, page.no 489-509
[3] S.j carter copper and gunns Tutorial pharmacy, page.no 341-352 .
[4] Mohon manuselis. Text book of diagnostic microbiology, 2nd edition, page. no 26-32.
[5] Tomasino, journals of AOAC international sep 1, 2006.
[6] w.h Hugo Journals of antimicrobial chemotherapy , vol 4, issue 6, page .no 489-494.
[7] A.D Russell, Journals of antimicrobial chemotherapy vol 49, issue 4, page .no 597-599.
[8] L.H. Cheah, biosecurity from prediction to damage control 130, New zealand plant protection 2009,
page .no 130-135.
[9] Suphota wattanaphansak Journals of suine health and production vol 18, nov 1, 2011.
[10]K. stringfellow, poultry science june 2009, vol 88, pg.no 1151-1155.
[11]T.Georgios, Applied and environmental microbiology, vol 67, dec 2011.
[12]T.Moretro, Journals of applied microbiology 2009,pg.no 1005-1012.
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