Anti-Adhesion and Anti-Biofilm Effectiveness of Disinfectants Used In Hemodialysis against both Staphylococcus Warneri and Staphylococcus Sciuri Biofilms
Biofilms are communities of microorganisms attached to a surface and included in an extracellular matrix making it resistant to exogenous deleterious agents. The aim of this study is to evaluate the anti-adhesive and anti-biofilm effect of five commercials disinfectants having different active principles (hydrogen peroxide, sodium hypochlorite, isopropyl alcohol and ethanol) on four Staphylococcus strains isolated from hemodialysis unit surfaces. The disinfectants anti-adhesive effect was estimated to an exceeding rate 70% for the various studied dilutions and 90% towards the pure products. Whereas the anti-biofilm effect showed an elimination rate varying between 10 % and 95 % according to the following parameters: active principle, time of contact, concentration and bacterial strain. Our study demonstrated that all tested products have an interesting anti-adhesive effect and that the peroxide of hydrogen is endowed with important anti-biofilm efficiency, followed by the alcoholic products and the sodium hypochlorite.
Synergistic effects of 18 flavonoids (11 glycosides and flavones, 01 flavones diglycoside, 04 chalcones and 02 aglycones) in combination with different anti-fungal agents against fungal strains were investigated. The agar diffusion assay of these flavonoids with different anti-fungal agents was tested. The Minimum Inhibitory Concentration (MIC) values of each of the flavonoids with different anti-fungal agents were determined by using checkerboard broth micro dilution assay. Flavones diglycoside (3, 5-dihydroxy flavones 7-O-b-D-glucuronide-4-O-b-D-glucopyranside) potentiated the in vitro and in vivo activity against fungal strains. The flavones diglycoside reduced MIC of amphotericin-B to one half against different fungal strains, Candida albicans, Candida krusei, Candida parapsilosis, Candida tropicalis and Cryptococcus neoformans 1202. Although moderate change between in vitro and in vivo studies have been found, the elucidation of the mechanisms involved in flavonoid action will have many health benefits to man. In conclusion, these findings suggested that flavonoid combination regimens may be considered as an useful candidate for the treatment of fungal infection.
Prof.Mr.Kiran K. Shinde (M.Pharm), Assistant professor (VNIPRC)
Pharmaceutical microbiology (Second year b.pharm) (3rd semester)
Introduction
Methods Of Different microbiological assays
Principles of Assays with Procedure
Methods For Standardization of
1. Antibiotics
2. Vitamins
3. Amino Acids
Assessment of new Antibiotic
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
Synergistic effects of 18 flavonoids (11 glycosides and flavones, 01 flavones diglycoside, 04 chalcones and 02 aglycones) in combination with different anti-fungal agents against fungal strains were investigated. The agar diffusion assay of these flavonoids with different anti-fungal agents was tested. The Minimum Inhibitory Concentration (MIC) values of each of the flavonoids with different anti-fungal agents were determined by using checkerboard broth micro dilution assay. Flavones diglycoside (3, 5-dihydroxy flavones 7-O-b-D-glucuronide-4-O-b-D-glucopyranside) potentiated the in vitro and in vivo activity against fungal strains. The flavones diglycoside reduced MIC of amphotericin-B to one half against different fungal strains, Candida albicans, Candida krusei, Candida parapsilosis, Candida tropicalis and Cryptococcus neoformans 1202. Although moderate change between in vitro and in vivo studies have been found, the elucidation of the mechanisms involved in flavonoid action will have many health benefits to man. In conclusion, these findings suggested that flavonoid combination regimens may be considered as an useful candidate for the treatment of fungal infection.
Prof.Mr.Kiran K. Shinde (M.Pharm), Assistant professor (VNIPRC)
Pharmaceutical microbiology (Second year b.pharm) (3rd semester)
Introduction
Methods Of Different microbiological assays
Principles of Assays with Procedure
Methods For Standardization of
1. Antibiotics
2. Vitamins
3. Amino Acids
Assessment of new Antibiotic
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
Effect of Some Disinfectants on Antibiotic Resistance Staphylococcus Isolated...iosrjce
IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS) is a double blind peer reviewed International Journal edited by the International Organization of Scientific Research (IOSR). The journal provides a common forum where all aspects of Agricultural and Veterinary Sciences are presented. The journal invites original papers, review articles, technical reports and short communications containing new insight into any aspect Agricultural and Veterinary Sciences that are not published or not being considered for publication elsewhere.
Antimicrobial activity of annona muricata L. (Soursop Leaves)Fattah Fazel
Testing antimicrobial activity of Annona Muricata L. (Soursop) using Disk diffusion method and Well diffusion method.
Bacteria used: MRSA, S.pyogenes, B. fragilis, C. perfringens
Note: No results. Reasons in the description section.
Biological Removal of Malachite Green and Congo red by Some Filamentous FungiIJEAB
Four strains of filamentous fungi were studied to a removal of Malachite green (MG) and Congo red (CR). These fungi were Aspergillus niger ,Aspergillus flavus , Aspergillus versicolor and P. funigulosum. P. funigulosum showed that decolorization activity was higher than other fungi on solid medium containing MG and CR . The stastical method obtained that there was no significance between fungi . All these fungi were able to degradation dyes to other metabolites . The dry weight ( Biomass) of P. funigulosum reached to 1.10 ,1.02 in mineral salts medium (MSM) with MG and CR respectively , and the stastical methods obtained that there was no significance in dry weights between fungi .
ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF ALGERIAN POPULUS NIGRA L. BUDS EX...bioejjournal
his study is part of a goal to investigate chemical composition, antibacterial, antifungal and antioxidant activities of the flower buds extracts from the Algerian Polulus nigra L., which were collected from Djarifet - mansourah at Tlemcen city in the West Northern of Algeria. In organic extracts, tanins, flavonoïds, coumarins, alkaloids and terpenoïds were the principals secondary metabolites identified from the flower buds of black poplar. Antibacterial and antifungal activities of extracts were tested using agar-well diffusion method and micro-well determination of MIC assay against eleven bacteria and two Candida species. It was found that extracts of black poplar buds exhibit antibacterial and anticandidal activities with agar disk diffusion (7 to 43mm) and MIC methods (MIC= 90.33 µg/ml against several strains of bacteria and MIC=45.16 µg/ml against Candida albicans). The antioxidant effect of hydroalcoholic extract was evaluated using DPPH and FRAP assays. It was showed good and similar activity than ascorbic acid and BHA by DPPH method: IC50= 220µg/mL for hydroethanol extract.
Antioxidant and Antimicrobial Activities Of Algerian Populus Nigra L. Buds Ex...bioejjournal
This study is part of a goal to investigate chemical composition, antibacterial, antifungal and antioxidant activities of the flower buds extracts from the Algerian Polulus nigra L., which were collected from Djarifet - mansourah at Tlemcen city in the West Northern of Algeria. In organic extracts, tanins, flavonoïds, coumarins, alkaloids and terpenoïds were the principals secondary metabolites identified from the flower buds of black poplar. Antibacterial and antifungal activities of
extracts were tested using agar-well diffusion method and micro-well determination of MIC assay against
eleven bacteria and two Candida species. It was found that extracts of black poplar buds exhibit
antibacterial and anticandidal activities with agar disk diffusion (7 to 43mm) and MIC methods (MIC=
90.33 µg/ml against several strains of bacteria and MIC=45.16 µg/ml against Candida albicans). The
antioxidant effect of hydroalcoholic extract was evaluated using DPPH and FRAP assays. It was showed good and similar activity than ascorbic acid and BHA by DPPH method: IC50= 220µg/mL for hydroethanol extract.
Comparative Study for Adsorptive Removal of Coralene Blue BGFS Dye from Aqueo...IJERA Editor
Textile industries represent biggest impact on the environment due to high water consumption and waste water discharge as government control water pollution by setting strength regulation for waste water discharge, removal of color from waste water becomes more and more essential and attractive. Adsorption technology is very efficient in treatment of textile effluent. In this paper comparison of adsorption phenomena of textile dye Anthraquinone blue onto two different adsorbents MgO nano powder and Fe2O3 amorphous powder has been studied for removal of said dye from aqueous solutions. The adsorption of Anthraquinone blue on adsorbents occurs by studying the effects of adsorbent amount, dye concentration, contact time and pH of solution. All results found that MgO nano powder and Fe2O3 provide a fairly high dye adsorption capacity, which combined with their fulfilment of pollution control board’s standards, lack of pollution, lower environmental hazard and low-cost makes them promising for future applications. The present work also provides information on optimum value of different operating parameter for dye removal by two adsorbent.
Adaptive Variable Structure Controller Application to Induction Motor DriveIJERA Editor
Variable structure control is an adaptive control that gives robust performance of a drive with parameter variation and load torque disturbance. Variable control structure is a robust control scheme based on the concept of changing the structure of the controller in response to the changing state of the system in order to obtain a desired response. The control is nonlinear and can be applied to the linear or nonlinear plant. A high speed switching control action is used to switch between different structures of the controller and the trajectory of the system is forced to move along a chosen switching manifold in the state space. The controller detects the deviation of the actual trajectory from the reference trajectory and corresponding changes the switching strategy to restore the tracking. Prominent characteristics such as invariance, robustness, order reduction, and control chattering are discussed in detail. Methods for coping with chattering are presented. Both linear and nonlinear systems are considered. By using Variable structure controller to control the step change in reference speed and drive system under load torque variations.
A Drift-Diffusion Model to Simulate Current for Avalanche Photo DetectorIJERA Editor
In this research, a Drift-Diffusion model is carried out to calculate includes impact ionization mechanism and can calculate dark current and photocurrent of avalanche photo diode. Poisson equation, electron and hole density continuity equations and electron and hole current equations have been solved simultaneously using Gummel method. Consideration of impact ionization enables the model to completely simulate the carriers flow in high electrical field. The simulation has been done using MATLAB and the results are compared with other reliable results obtained by researchers. Our results show despite of hydrodynamics and Monte Carlo methods which are very complicated we can get the current characteristics of photo detector easily with acceptable accuracy. In addition we can use this method to calculate currents of device in high fields.
Effects of Irrigation Practices on Some Soil Chemical Properties on OMI Irrig...IJERA Editor
Irrigation practices have been observed to impact scheme soil properties and other parameters negatively. These could be as a result of irrigation water quality, method of application and nature of scheme soil. This study was therefore conducted to study the effects of irrigation practices on the soils of Omi irrigation scheme Kogi state, Nigeria after 13years of operation. Soil samples were taken at depths 0 – 20 cm (A1), 20 – 80 cm (A2) and 80 – 120 cm (A3) from two operating lands (OL); OL 5 and OL 18 of the study area. The samples were analysed for chemical parameters (pH, CEC, ESP, Mg2+, Ca2+, OM, and OC). The soil pH which was in the neutral range (pH=6.65 to 7.00) at inception of scheme, has become slightly acidic (pH=6.53 to 6.60). Cation exchange capacity (CEC) levels have also increased from 10cmol+kg-1 to 35cmol+kg-1. While Organic matter (OM) and Organic carbon (OC) also have marked increase in their levels (baseline as 0.93 to 1.08; for year 2013 as 9.52 to 9.79). Generally, the analysis indicated a need for proper monitoring of the scheme soil to prevent further deterioration.
Effect of Some Disinfectants on Antibiotic Resistance Staphylococcus Isolated...iosrjce
IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS) is a double blind peer reviewed International Journal edited by the International Organization of Scientific Research (IOSR). The journal provides a common forum where all aspects of Agricultural and Veterinary Sciences are presented. The journal invites original papers, review articles, technical reports and short communications containing new insight into any aspect Agricultural and Veterinary Sciences that are not published or not being considered for publication elsewhere.
Antimicrobial activity of annona muricata L. (Soursop Leaves)Fattah Fazel
Testing antimicrobial activity of Annona Muricata L. (Soursop) using Disk diffusion method and Well diffusion method.
Bacteria used: MRSA, S.pyogenes, B. fragilis, C. perfringens
Note: No results. Reasons in the description section.
Biological Removal of Malachite Green and Congo red by Some Filamentous FungiIJEAB
Four strains of filamentous fungi were studied to a removal of Malachite green (MG) and Congo red (CR). These fungi were Aspergillus niger ,Aspergillus flavus , Aspergillus versicolor and P. funigulosum. P. funigulosum showed that decolorization activity was higher than other fungi on solid medium containing MG and CR . The stastical method obtained that there was no significance between fungi . All these fungi were able to degradation dyes to other metabolites . The dry weight ( Biomass) of P. funigulosum reached to 1.10 ,1.02 in mineral salts medium (MSM) with MG and CR respectively , and the stastical methods obtained that there was no significance in dry weights between fungi .
ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF ALGERIAN POPULUS NIGRA L. BUDS EX...bioejjournal
his study is part of a goal to investigate chemical composition, antibacterial, antifungal and antioxidant activities of the flower buds extracts from the Algerian Polulus nigra L., which were collected from Djarifet - mansourah at Tlemcen city in the West Northern of Algeria. In organic extracts, tanins, flavonoïds, coumarins, alkaloids and terpenoïds were the principals secondary metabolites identified from the flower buds of black poplar. Antibacterial and antifungal activities of extracts were tested using agar-well diffusion method and micro-well determination of MIC assay against eleven bacteria and two Candida species. It was found that extracts of black poplar buds exhibit antibacterial and anticandidal activities with agar disk diffusion (7 to 43mm) and MIC methods (MIC= 90.33 µg/ml against several strains of bacteria and MIC=45.16 µg/ml against Candida albicans). The antioxidant effect of hydroalcoholic extract was evaluated using DPPH and FRAP assays. It was showed good and similar activity than ascorbic acid and BHA by DPPH method: IC50= 220µg/mL for hydroethanol extract.
Antioxidant and Antimicrobial Activities Of Algerian Populus Nigra L. Buds Ex...bioejjournal
This study is part of a goal to investigate chemical composition, antibacterial, antifungal and antioxidant activities of the flower buds extracts from the Algerian Polulus nigra L., which were collected from Djarifet - mansourah at Tlemcen city in the West Northern of Algeria. In organic extracts, tanins, flavonoïds, coumarins, alkaloids and terpenoïds were the principals secondary metabolites identified from the flower buds of black poplar. Antibacterial and antifungal activities of
extracts were tested using agar-well diffusion method and micro-well determination of MIC assay against
eleven bacteria and two Candida species. It was found that extracts of black poplar buds exhibit
antibacterial and anticandidal activities with agar disk diffusion (7 to 43mm) and MIC methods (MIC=
90.33 µg/ml against several strains of bacteria and MIC=45.16 µg/ml against Candida albicans). The
antioxidant effect of hydroalcoholic extract was evaluated using DPPH and FRAP assays. It was showed good and similar activity than ascorbic acid and BHA by DPPH method: IC50= 220µg/mL for hydroethanol extract.
Comparative Study for Adsorptive Removal of Coralene Blue BGFS Dye from Aqueo...IJERA Editor
Textile industries represent biggest impact on the environment due to high water consumption and waste water discharge as government control water pollution by setting strength regulation for waste water discharge, removal of color from waste water becomes more and more essential and attractive. Adsorption technology is very efficient in treatment of textile effluent. In this paper comparison of adsorption phenomena of textile dye Anthraquinone blue onto two different adsorbents MgO nano powder and Fe2O3 amorphous powder has been studied for removal of said dye from aqueous solutions. The adsorption of Anthraquinone blue on adsorbents occurs by studying the effects of adsorbent amount, dye concentration, contact time and pH of solution. All results found that MgO nano powder and Fe2O3 provide a fairly high dye adsorption capacity, which combined with their fulfilment of pollution control board’s standards, lack of pollution, lower environmental hazard and low-cost makes them promising for future applications. The present work also provides information on optimum value of different operating parameter for dye removal by two adsorbent.
Adaptive Variable Structure Controller Application to Induction Motor DriveIJERA Editor
Variable structure control is an adaptive control that gives robust performance of a drive with parameter variation and load torque disturbance. Variable control structure is a robust control scheme based on the concept of changing the structure of the controller in response to the changing state of the system in order to obtain a desired response. The control is nonlinear and can be applied to the linear or nonlinear plant. A high speed switching control action is used to switch between different structures of the controller and the trajectory of the system is forced to move along a chosen switching manifold in the state space. The controller detects the deviation of the actual trajectory from the reference trajectory and corresponding changes the switching strategy to restore the tracking. Prominent characteristics such as invariance, robustness, order reduction, and control chattering are discussed in detail. Methods for coping with chattering are presented. Both linear and nonlinear systems are considered. By using Variable structure controller to control the step change in reference speed and drive system under load torque variations.
A Drift-Diffusion Model to Simulate Current for Avalanche Photo DetectorIJERA Editor
In this research, a Drift-Diffusion model is carried out to calculate includes impact ionization mechanism and can calculate dark current and photocurrent of avalanche photo diode. Poisson equation, electron and hole density continuity equations and electron and hole current equations have been solved simultaneously using Gummel method. Consideration of impact ionization enables the model to completely simulate the carriers flow in high electrical field. The simulation has been done using MATLAB and the results are compared with other reliable results obtained by researchers. Our results show despite of hydrodynamics and Monte Carlo methods which are very complicated we can get the current characteristics of photo detector easily with acceptable accuracy. In addition we can use this method to calculate currents of device in high fields.
Effects of Irrigation Practices on Some Soil Chemical Properties on OMI Irrig...IJERA Editor
Irrigation practices have been observed to impact scheme soil properties and other parameters negatively. These could be as a result of irrigation water quality, method of application and nature of scheme soil. This study was therefore conducted to study the effects of irrigation practices on the soils of Omi irrigation scheme Kogi state, Nigeria after 13years of operation. Soil samples were taken at depths 0 – 20 cm (A1), 20 – 80 cm (A2) and 80 – 120 cm (A3) from two operating lands (OL); OL 5 and OL 18 of the study area. The samples were analysed for chemical parameters (pH, CEC, ESP, Mg2+, Ca2+, OM, and OC). The soil pH which was in the neutral range (pH=6.65 to 7.00) at inception of scheme, has become slightly acidic (pH=6.53 to 6.60). Cation exchange capacity (CEC) levels have also increased from 10cmol+kg-1 to 35cmol+kg-1. While Organic matter (OM) and Organic carbon (OC) also have marked increase in their levels (baseline as 0.93 to 1.08; for year 2013 as 9.52 to 9.79). Generally, the analysis indicated a need for proper monitoring of the scheme soil to prevent further deterioration.
Productivity Improvement by Applying DILO (Time and Motion) and Lean PrinciplesIJERA Editor
In recent years, service industries are struggling to improve their processes efficiencies. Lean principles and the methodologies are becoming a prime problem solving approaches to perform the operational processes with a minimum non-value adding activities resulting in reduced wait time, movements, defect rates and other delays in the process. This paper addresses the implementation of lean principles in a one of the service organizations. The main objective of this paper is to draw the As-Is process map, conduct process analysis to identify non-value added activities, capture the time using DILO (day in a life of…), simplify or eliminate the same and improve efficiency of the process. Through the application of lean principles the As-Is and To-Be process maps are constructed to improve the operational processes by identifying different waste and its sources of wastes. A noticeable reduction in processing time is observed in the process by simplifying and eliminating the non value added activities in the process. This case study can be useful in developing a more generic approach to design lean environment
Mathematical Modeling Of Syphilis Disease A Case Study With Reference To Anan...IJERA Editor
In this paper we have analyzed the Mathematical modeling of Syphilis disease, Syphilis is a highly contagious disease spread primarily by sexual activity, including oral and anal sex. Occasionally, the disease can be passed to another person through prolonged kissing or close bodily contact. Although this disease is spread from sores, the vast majority of those sores go unrecognized. The infected person is often unaware of the disease and unknowingly passes it on to his or her sexual partner. Pregnant women with the disease can spread it to their baby. This disease, called congenital syphilis, can cause abnormalities or even death to the child. Syphilis cannot be spread by toilet seats, door knobs, swimming pools, hot tubs, bath tubs, shared clothing, or eating utensils.
Effect of simultaneous movement of multiple particles under electric field in...IJERA Editor
20% of failures in Gas Insulated Substations are due to the existence of various metallic contaminations in the form of loose particles. These particles may be free to move in the electric field or may be fixed on the conductors, thus enhancing local surface fields. In this paper a Single Phase Gas Insulated Bus duct with inner and outer diameter to 55mm and 152 mm is considered. In this paper multiple particles i.e three particle of different sizes are considered. These three particles are assumed to be rest at a position. Basic equations for the movement of three metallic particles are formulated. The motion of the three particles are simulated for different voltages using MATLAB. Effect of multiple particles for various electric fields on particle movement are analyzed and time of collisions of the particle at first time is determined for various voltages. Particles of copper of 10 mm in length and 0.25 mm radius, 10 mm length and 0.15 mm radius and 7 mm and 0.25 radius are considered. The results show that the three particle collide at different points depending on the particles positions. The simulation results have been presented and analyzed.
Design Approach for Vehicle To Vehicle (V2V) Dissemination of Messages in Veh...IJERA Editor
Designing a protocol structure which contains the control system, VANET device and the type of communication message structures which will implement the message packets for v2v or v2I infrastructure . The different message packets contain the information required to transfer from one node to another in vehicular network for communication. Design various algorithm required for Control, Alert and Infotainment messages. Time stamping lowering data rate efficient packet delivery and proper communication of required messages. Implementation of proposed algorithms and comparison of them with existing algorithms and study of different factors affecting the working of theses algorithms. Analysis of proposed design approach and improvements in results if required.
A Survey of String Matching AlgorithmsIJERA Editor
The concept of string matching algorithms are playing an important role of string algorithms in finding a place where one or several strings (patterns) are found in a large body of text (e.g., data streaming, a sentence, a paragraph, a book, etc.). Its application covers a wide range, including intrusion detection Systems (IDS) in computer networks, applications in bioinformatics, detecting plagiarism, information security, pattern recognition, document matching and text mining. In this paper we present a short survey for well-known and recent updated and hybrid string matching algorithms. These algorithms can be divided into two major categories, known as exact string matching and approximate string matching. The string matching classification criteria was selected to highlight important features of matching strategies, in order to identify challenges and vulnerabilities.
Zigbee Based Indoor Campus Inventory Tracking Using Rfid ModuleIJERA Editor
This is a very useful application of RFID (Radio-frequency identification) and is very commonly used in institutes, offices, homes and so on. An RFID system consists of a reader device and a transponder. A transponder or tag has a unique serial number which is identified by the reader. Here RFID has been interfaced with ARM Processor to provide secured access. The relevant messages are also displayed on a 16x2 LCD.RFID automated access for door controls to buildings, departments, rooms, secured closets (wiring, PBX, etc.) and cabinets is very cost effective and secure to use. Many people do not realize how easy it is to implement card access systems such as card access door or doors using RFID readers and RFID Cards or Key fobs for Secured Access Control Management. You can even use smart readers for computer rooms and securing individual computers. RFID tags are categorized as either active or passive. Active tags are powered by an internal battery and are typically read/write,i.e tag data can be rewritten or modified. Passive tags operate without a separate external power source and obtain operating power generated from the reader.
Pressure Gradient Influence on MHD Flow for Generalized Burgers’ Fluid with S...IJERA Editor
This paper presents a research for magnetohydrodynamic (MHD) flow of an incompressible generalized
Burgers’ fluid including by an accelerating plate and flowing under the action of pressure gradient. Where the
no – slip assumption between the wall and the fluid is no longer valid. The fractional calculus approach is
introduced to establish the constitutive relationship of the generalized Burgers’ fluid. By using the discrete
Laplace transform of the sequential fractional derivatives, a closed form solutions for the velocity and shear
stress are obtained in terms of Fox H- function for the following two problems: (i) flow due to a constant
pressure gradient, and (ii) flow due to due to a sinusoidal pressure gradient. The solutions for no – slip condition
and no magnetic field, can be derived as special cases of our solutions. Furthermore, the effects of various
parameters on the velocity distribution characteristics are analyzed and discussed in detail. Comparison between
the two cases is also made.
Virulence Phenotype, Physicochemical Properties and Biofilm Formation of Pseu...IJERA Editor
Potable water piping has been demonstrated to serve as a reservoir for opportunistic pathogens bacteria such as Pseudomonas aeruginosa. In this report, we describe the characterization of P. aeruginosa strains isolated from water intended for human consumption by the presence of virulence factors. These strains expressed their suitability for adhesion and the formation of biofilms on polyethylene (PE). Also In this work, we were able to elucidate the factors intervening in adhesion and biofilm formation by showing the role of the substrate, the environment and bacteria. Strong correlation was observed between physicochemical properties especially the electron donor property and the surface percentage covered by cells. These results indicate that this property plays a crucial role in Pseudomonas aeruginosa adherence on the PE surface. In addition, if no relationship was found between the adhesion results and hydrophobicity, it means that this property was not involved in the adhesion process of Pseudomonas aeruginosa on the PE surface.
MoO3 Nanoparticles: Synthesis, Characterization and Its Hindering Effect on G...IJERA Editor
Molybdenum trioxide nanoparticles have been synthesized by sol-gel method. The X-ray diffraction analysis was done to confirm that the obtained product was MoO3. The scanning electron microscopy was done to study the shape, size distribution and surface morphology of nanoparticles; they had a hexagonal shape with smooth surface and uniform size distribution. The functional groups were studied using Fourier transform infrared spectroscopy. The effect of MoO3 nanoparticles on seed germination of vigna unguiculata was studied for 6 days from the day of sowing, by comparing the time taken for seeds to germinate and length of shoot with respect to time of the seeds sowed in heavy black soil whose nutrient composition was known with seeds sowed in the same heavy black soil but which was made rich with MoO3 nanoparticles. It was observed that the MoO3 nanoparticles hampered the germination of vigna unguiculata seeds and this restraint continued in the shoot growth also.
Channel Estimation Techniques in MIMO-OFDM LTE SystemsCauses and Effects of C...IJERA Editor
There is an increasing demand for high data transmission rates with the evolution of the very large scale integration (VLSI) technology. The multiple input multiple output-orthogonal frequency division multiplexing (MIMO-OFDM) systems are used to fulfill these requirements because of their unique properties such as high spectral efficiency, high data rate and resistance towards multipath propagation. MIMO-OFDM systems are finding their applications in the modern wireless communication systems like IEEE 802.11n, 4G and LTE. They also offer reliable communication with the increased coverage area. The bottleneck to the MIMO-OFDM systems is the estimation of the channel state information (CSI). This can be estimated with the help of any one of the Training Based, Semiblind and Blind Channel estimation algorithms. This paper presents various channel estimation algorithms, optimization techniques and their effective utilization in MIMO-OFDM for modern wireless LTE systems.
Automatic Recognition of Isolated And Interacting Manufacturing Features In M...IJERA Editor
Manufacturing features play an important role between design information and manufacturing activities.
Recently, various efforts have been concentrated in development of automatic feature recognition systems.
However, only limited number of features could be recognized, intersecting features were generally not
involved. This paper presents a simple system, in which manufacturing features are easily detected using a
Chain of Faces and Base of Faces (CF-BF) graph. A feature is modeled by a series/parallel association of
opened Chain of Faces (OCF) or Closed chain of Faces (CCF) that rest on a Base Face (BF). The feature is
considered Perfect Manufacturing Feature (PMF) if all Faces that participate in constitution of OCF/CCF are
blank faces, else it is an Imperfect Manufacturing Feature (IMF). In order to establish news Virtual Faces to
satisfy this necessaries condition, a judicious analysis of orientation of frontier faces that rest on BF is
performed. The technique was tested on several parts taken from literature and the results were satisfying.
Research on Iris Region Localization AlgorithmsIJERA Editor
Iris recognition is a biometric technique that offers premium performance. Iris localization is critical to the success of an iris recognition system, since data that is falsely represented as iris pattern data will corrupt the biometric templates generated, resulting in poor recognition rates. So far different algorithms for iris localization having been proposed. This paper explored four efficient methods for iris localization, out of these three methods of iris localization in circular form and one methods of unwrapping the iris in to a flat bed. Experimental results are reported to demonstrate performance evaluation of every implemented algorithms. Conclusion based on comparisons can provide most significant information for further research. A CASIA and UPOL iris databases of iris images has been used for implementation of iris localization General Term Biometrics,Iris Recognition,Iris Localization
The application of queue theory in cloud computing to reduce the waiting timeIJERA Editor
Cloud computing is a new technology in computer field to provide on line service to the customers. -Cloud computing has got enormous popularity as it offers dynamic, low-cost computing solutions. To get the service of cloud the user has to be in queue until he is served. Each arriving Cloud computing User (CCU) requests Cloud computing Service Provider (CCSP) to use the resources, if server is available, the arriving user will seize and hold it for a length of time, which leads to queue length and more waiting time. A new arrival leaves the queue with no service. After service completion the server is made immediately available to others. From the user’s point of view he needs to be served immediately and to prevent waiting the CCSP’s can use infinite servers to reduce waiting time & queue length. The arrival pattern is often Poisson in queuing theory. In this article we analyzed the dynamic behavior of the system with infinite servers by finding various effective measures like response time, average time spend in the system, utilization and throughput.
Examination passive defense role in spatial distribution of urban regionIJERA Editor
Cities due to the compact and densely populated urban context and diverse urban networks usually are considered in the wars against ground and air attacks as a barrier. And on the other hand, struggles wanted or unwanted wars have led to the cities and sometimes causing high civilian casualties and damage to the city. Expert and track record militaries believe that urban warfare is one of the hardest battles of the model because of the possibility of resistance in the cities due to high intensive residential context and use different weapons to militants in their application package is limited. However, the military forces moved in is very difficult and may well lead them to the attacker's losses also increase under these conditions. Research methodology in this study was a descriptive - analytical. In this paper, after introducing key items affecting the spatial distribution and role of each one in the establishment and development of urban areas first, it will pay the expression of passive defense and civil defense in Tehran metropolitan area and second, to analyze the role of passive defense and settlement of the spatial distribution of urban infrastructure deals and the strengths and weaknesses of the studies discussed and finally, after a review and analysis of all factors will pay conclusions and provide effective strategies taking into account the spatial distribution and effective passive defense will be the key factor.
Fabrication, Designing & Performance Analysis of Solar Parabolic TroughIJERA Editor
A parabolic trough solar collector uses a parabolic cylinder to reflect and concentrate sun radiations towards a receiver tube located at the focus line of the parabolic cylinder. The receiver absorbs the incoming radiations and transforms them into thermal energy, the latter being transported and collected by a fluid medium circulating within the receiver tube.This method of concentrated solar collection has the advantage of high efficiency and low cost, and can be used either for thermal energy collection, for generating electricity or for both, This paper focused on the fabrication and designing of solar parabolic trough, The designing of trough is depend upon the following parameters : Aperture of the concentrator , Inner diameter of absorber tube, Outer diameter of absorber tube, Inner diameter of glass tube, Outer diameter of glass tube, Length of parabolic trough, Concentration ratio, Collector aperture area, Specular reflectivity of concentrator, Glass cover transitivity for solar radiation, Absorber tube emissivity/emissivity, Intercept factor, Emissivity of absorber tube surface and Emissivity of glass. The performance analysis will be based on the Experimental data collection and calculations with reference to: Thermal performance calculations, Overall loss coefficient and heat correlations. Heat transfer coefficient on the inside surface of the absorber tube and Heat transfer coefficient between the absorber tube and the Cover.
Fabrication, Designing & Performance Analysis of Solar Parabolic Trough
Similar to Anti-Adhesion and Anti-Biofilm Effectiveness of Disinfectants Used In Hemodialysis against both Staphylococcus Warneri and Staphylococcus Sciuri Biofilms
DETERMINATION OF PATHOGENICITY AND VIRULENCE OF A COMBINATION OF STRAINS OF Beauveria bassiana, Metarhizium anisopliae and Paecilomyces fumosoroseus OF THE COMPANY SANOPLANT, ON THE PSYLLID Diaphorina citri VECTOR OF THE HLB DISEASE OF CITRUS
Degradation of Nevirapine and Trimethoprim from Aqueous Solutions using Selec...Agriculture Journal IJOEAR
Together with pharmaceutical residues, personal care products encompassing prescription drugs, fragrances, and cosmetics have been detected in groundwater and other aquatic environments, hence compromising the quality of water. Their classification as micropollutants is due to their antibacterial resistance potential, persistence, and ecotoxicity. Biodegradation has been identified as a potential mechanism in their removal. The focus of this study focus was bioaugmentation; (Bacillus subtilis, Escherichia coli, Staphylococcus aureus, and Pseudomonas aeroginosa) to enhance the degradation of Nevirapine and Trimethoprim in model aqueous solutions. A liquid chromatography-tandem mass spectrometer (LC-MS/MS) was used to determine the pharmaceuticals. The efficacy of the bacterial strains to degrade selected drugs was evaluated by making the two drugs the sole source of energy and carbon. From the experimental data, the highest percentage biodegradation was recorded; Pseudomonas aeroginosa (86 %) and Staphylococcus aureus (79 %) for TMP and NVP respectively.
5.1.3. Efficacy of antimicrobial preservation (EP 5.0)Guide_Consulting
Salah Satu Referensi Yang Digunakan Dalam One Day Seminar "Preservative Effectiveness Validation" 04 Desember 2014.
Detail : info@traininglaboratorium.com
Testing the ability against Bacillus cereus of actinobacteria strains isolate...Agriculture Journal IJOEAR
This study aimed to test the antibacterial activity of Bacillus cereus of actinobacterial isolates isolated from marine sponges in the Kien Giang Sea, Vietnam. That can select the strains with high resistance to identify them. There were 198 actinobacterial isolates tested. Based on the ability of antimicrobial activity to B. cereus, 82/198 had the against B. cereus, in which there were six isolates with high (7.3%), 52 medium (25.6%), and 21 weak resistance (67.1%). Selection of six isolates with the best resistance to B. cereus (ND1.7a, ND2.7c, HD1-3e, HD1-6a, HD2.3b, and H6b) identified by PCR and 16S rRNA gene sequencing. The results identified five strains of Streptomyces (Streptomyces tateyamensis ND1.7a, Streptomyces althioticus HD1.3e, Streptomyces flaveolus HD1.6a, Streptomyces olivaceus HD2.3d, and Streptomyces albidoflavus H6b) and one strain of genus Microbacterium (Microbacterium tumbae ND2.7c).
Evaluation of the Antibacterial Activities of Crude Extracts of Cochlospermum...iosrjce
The antibacterial activities of the methanolic, hot water, chloroform and petroleum ether extracts of
Choclospermum planchonii leaf were tested against some clinical bacterial isolates and reference organisms.
The phytochemical screenings of the extracts were also carried out. The leaf extracts of the plant exhibited
antibacterial activities against clinical isolates of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus
aureus, Shigella flexneri, and Salmonella typhii and reference strains of Staphylococcus aureus (ATCC 28923),
Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853. The methanolic leaf extracts
exhibited the highest antibacterial activity, its minimum inhibitory concentration (MIC) ranging between 0.31
and 1.25 mg/ml; and its zones of inhibition on the various test microorganisms ranging between 8mm and 13
mm. The petroleum ether extracts had the weakest antibacterial activity, its minimum inhibitory concentration
(MIC) ranging between 2.50mg/ml and 5.00mg/ml and its inhibition zone ranging between 4mm and 8mm. The
phytochemical constituents present in the plant were alkaloids, tannins, saponins, cardiac glycosides, and
sterols. The methanolic extracts of the leaf exhibited potentially biological activity than other extracts. This
indicates that the plant leaf could be useful in the treatment of human infections caused by these pathogens. The
antibacterial activities of the methanolic leaf extract may be attributed to the presence of bioactive compounds.
In vitro controlling of selected human diarrhea causing bacteria by clove ext...Open Access Research Paper
Antibacterial activity of clove extracts (Syzygium aromaticum L.) was proven against five diarrhea causing bacteria. This was further confirmed when compared with commonly used three commercial antibiotics (ciprofloxacin, tetracycline and erythromycin) as a positive control. Significant differences (P<0.0001) were observed in the effect of the antimicrobial agents (clove extracts and antibiotics), and in the sensitivities of the bacterial species (P<0.0001) to the antimicrobial agents. Clove extracts had significant (P<0.001) activity with the acetone extract demonstrating highest activity followed by antibiotics and other extracts against tested bacteria. The zone of inhibition of clove extracts was ranged from 7.33 to 12.00 mm whereas in antibiotics, it was 0.00 to 11.67 mm. Of all the bacteria, Salmonella typhimurium was the most susceptible against all of the extracts as well as concentrations of clove, while low MIC (180 mgml-1) and MBC (680 mgml-1) of the extracts were observed against Shigella dysenteriae. Consequently, clove has a significant antidiarrheal activity and it could be used as an effective antibacterial agent, alternative to the use of antibiotics.
— Post-harvest diseases are a major problem for banana yield. Despite treatments with chemical fungicides, a persistence of diseases is noticed. This study aims at proposing a biological control method against banana post-harvest diseases by using banana rachis leachate. The effect of leachate has been tested in vitro on mycelial growth, conidial germination and in vivo on pathogenic fungi virulence. All leachate concentrations (5, 15 and 20%) tested showed antifungal activity on the tested fungi. However, the 20% concentration was more effective with complete inhibition of mycelial growth and conidial germination of all fungi. No symptoms of crown rot and anthracnose were observed after treatment of bananas with leachate. However, with azoxystrobin, the prevalence of crown rot and anthracnose was 60% and 30%, respectively. Banana rachis leachate recorded highly significant reduction of banana finger rot prevalence compared to azoxystrobin. Banana rachis leachate have strong antifungal properties that may be useful to control banana post-harvest disease as a safe alternative option to chemical fungicides Keywords— banana; post-harvest diseases; banana rachis leachate, antifungal activity.
The effect of silver nanoparticles on Staphylococcus epidermidis biofilm biom...Nanomedicine Journal (NMJ)
Abstract
Objective(s):
Bacterial biofilm has been considered responsible for many deaths and high health costs worldwide. Their better protection against antibacterial agents compared to free living cells leads to poor treatment efficiency. Nanotechnology is promising approach to combat biofilm infections. The aim of the present study was to eradicate Staphylococcus epidermidis biofilm with silver nanoparticles (SNPs).
Materials and Methods:
SNPs were used at different concentrations (two fold dilutions) and incubation times (24, 48, 72 h). The crystal violet staining and pour plate assays were used to assess biofilm biomass and bacterial viability, respectively. The ability of SNPs on biofilm matrix eradication was assessed through optical density ratio (ODr). Positive control was defined as an ODr =1.0.
Results:
The crystal violet assay indicated that the biofilm matrixes were intact at different concentrations of SNOs and incubation times. There were no significant differences between these parameters (P >0.05). Bacterial enumeration studies revealed that higher concentrations of SNPs were more effective in killing bacteria than lower ones. Although, longer incubation times led to enhancement of anti-biofilm activity of SNPs.
Conclusion:
The anti-biofilm activity of SNPs was concentration- and time-dependent. The results of this study highlighted that SNPs were effective against cell viability; however they were ineffective against biomass.
Effect of plant growth promoting rhizobacterial (PGPR) inoculation on growth ...IJEAB
Plant Growth promoting rhizobacteria are a heterogeneous group of bacteria that can be found in the rhizosphere, at root surfaces and in association with roots. They benefit plants through Production of plant hormones, such as auxins, asymbiotic N2 fixation, solubilization of mineral phosphates, antagonism against phytopathogenic microorganisms by production of antibiotics, siderophroes, Chitinase and other nutrients ability to effectively colonize roots are responsible for plant growth promotion. An experiment was conducted in the field of National Institute of Agronomic Research of Meknes. Morocco. The experiment was a completely randomized design with six replicates. There were four treatments viz. T1: (control; N0 -PGPR), T2: (N0 +2027-2), T3: (N0 +2066-7) and T4: (N0+2025-1). The results indicated that a remarkable increase in root growth, namely length, the diameter of the rod and the total chlorophyll. A total of three different bacteria colonies were isolated and proceed with in vitro screening for plant growth promoting activities; phosphate solubilization, nitrogen fixation, indole acetic acid (IAA), ammonia production and antimicrobial enzymes (cellulose, chitinase and protease) activity. Among the three bacterial strains, all bacterial strains are able to produce ammonia, IAA production and nitrogen fixation activity, one strain phosphate solubilizing activity, two strain are able to produce cellulase syntheses, Protease activity and Chitinase activity.
The effect of two isolated Bacillus thuringiensis Bt ,HD 210 and Bt HD128. were tested against the olive fruit pest Bactrocera oleae . data calculated , show that, the
Lc50 recoded , 128 and 137 Ug/ml Bactrocera oleae treated with different concentrations of Bt ,HD 210 and Bt HD128, respectively. The experiments carried out
under field conditions during two successive seasons 2015 and 2016. The results obtained show that during two panting seasons, 2015 and 2016 the infestations of the
olive trees with B. oleae were significantly decreased to 11±4.2 and 30±4.2 individuals after treated with the bacteria Bt ,HD 210 and Bt HD128, respectively as
compared to 99±9.9 individuals in the control during season 2015 after 120 days of treatments. During season 2016 the infestations significantly decreased to 17±9.9
and 33±9.4 individuals after treated with the corresponding pathogens as compared to 99±8.4 individuals in the control.
At the harvest time during season 2015, the weight of the olive fruits were significantly increased to 2777± 72.82, and 2831± 12.51 Kg/ feddan in plots treated with Bt,
HD 210 and Bt HD128, respectively as compared 2110± 12.51Kg/feddan in the control. During season 2016 the olive fruit significantly increased to Kg/ feddan in
plots treated with Bt ,HD 210.
Antimicrobial Activity of Bauhinia Purpurea (L) by Minimum Inhibitory Concent...IOSRJPBS
MIC methods are widely used in the comparative testing of new agents, or when a more accurate result is required for clinical management. As there are no CLSI (formerly NCCLS) recommendations for the determination of MICs of varies bio-compounds against gram positive and gram negative organisms. The minimum inhibitory concentration is the lowest concentration (in μg/ml) of an antibiotic that inhibits the growth of a given strain of bacteria. In Diagnostic laboratories these MIC’S are used to confirm resistance and also most often used to determine antimicrobial invitro activity. In the present MIC method, one can get the information about Antibiotic agent, preparation of stock solutions, media and inoculation, conditions of incubation, ultimately reading and interpretation of results. The present study is focused to evaluate antibacterial activities of ethanolic extract and purified fractions of Bauhinia purpurea using a gram-positive and a gram-negative organisms Staphylococcus aureus , Klebsiella, E.coli and enterococcus
ANTIBACTERIAL ACTIVITY OF Citrus limonON Acnevulgaris (PIMPLES) IJSIT Editor
Research was carried out on antibacterial activity of Citrus limon on Acnevulgaris (Pimples). Samples
were obtained from individuals having Pimples, by swabbing their faces, backs and chests. Samples were
collected from Amanawa hospital in sokoto, Nigeria using Swab sticks. The sticks were transported to the
Microbiology Laboratory of the Usmanu Danfodiyo University Sokoto. Citrus limon juice was used at different
concentrations of (20%, 40%, 60%, 80% and 100%) on Propionibacteriumacnes, the bacteria that cause
Acnevulgaris (Pimples). The Citrus limon juice was found to be effective at all Concentrations used.
Conventional Cleanser was used as positive control, and it was only found to be effective at higher
concentrations of (60%, 80% and 100%) and was not effective at Lower Concentrations (20% and 40%). The
Minimum Inhibitory Concentration (MIC) of Citrulimon on Propionibacterium acnes was taken and presence
of growth was observed at concentrations of 20%, 40% and 60%, and absence of growth was observed at
80% and 100%. The minimum inhibitory concentration of conventional cleanser indicated the presence of
growth at 20% and 40% and absence of Growth at 60%, 80% and 100%. The Minimum bactericidal
Concentration (MBC) taken on Propionibacteriumacnes using both Citrus limon juice and cleanser all showed
absence of growth at all the concentrations used (20%, 40%, 60%, 80% and 100%). From the research
conducted it was observed that lemon juice have strong anti Acne vulgaris effect morethan the convensional
cleansers used for the treatment of Acne vulgaris.
Phytochemical screening and antibacterial properties from extract of Alchorne...Uploadworld
This study involved a survey on the use of extract of Alchornea cordifolia a medicinal plant used locally in Cameroon as traditional medicine for the treatment of infectious diseases.
Similar to Anti-Adhesion and Anti-Biofilm Effectiveness of Disinfectants Used In Hemodialysis against both Staphylococcus Warneri and Staphylococcus Sciuri Biofilms (20)
Quality defects in TMT Bars, Possible causes and Potential Solutions.PrashantGoswami42
Maintaining high-quality standards in the production of TMT bars is crucial for ensuring structural integrity in construction. Addressing common defects through careful monitoring, standardized processes, and advanced technology can significantly improve the quality of TMT bars. Continuous training and adherence to quality control measures will also play a pivotal role in minimizing these defects.
Saudi Arabia stands as a titan in the global energy landscape, renowned for its abundant oil and gas resources. It's the largest exporter of petroleum and holds some of the world's most significant reserves. Let's delve into the top 10 oil and gas projects shaping Saudi Arabia's energy future in 2024.
Explore the innovative world of trenchless pipe repair with our comprehensive guide, "The Benefits and Techniques of Trenchless Pipe Repair." This document delves into the modern methods of repairing underground pipes without the need for extensive excavation, highlighting the numerous advantages and the latest techniques used in the industry.
Learn about the cost savings, reduced environmental impact, and minimal disruption associated with trenchless technology. Discover detailed explanations of popular techniques such as pipe bursting, cured-in-place pipe (CIPP) lining, and directional drilling. Understand how these methods can be applied to various types of infrastructure, from residential plumbing to large-scale municipal systems.
Ideal for homeowners, contractors, engineers, and anyone interested in modern plumbing solutions, this guide provides valuable insights into why trenchless pipe repair is becoming the preferred choice for pipe rehabilitation. Stay informed about the latest advancements and best practices in the field.
Final project report on grocery store management system..pdfKamal Acharya
In today’s fast-changing business environment, it’s extremely important to be able to respond to client needs in the most effective and timely manner. If your customers wish to see your business online and have instant access to your products or services.
Online Grocery Store is an e-commerce website, which retails various grocery products. This project allows viewing various products available enables registered users to purchase desired products instantly using Paytm, UPI payment processor (Instant Pay) and also can place order by using Cash on Delivery (Pay Later) option. This project provides an easy access to Administrators and Managers to view orders placed using Pay Later and Instant Pay options.
In order to develop an e-commerce website, a number of Technologies must be studied and understood. These include multi-tiered architecture, server and client-side scripting techniques, implementation technologies, programming language (such as PHP, HTML, CSS, JavaScript) and MySQL relational databases. This is a project with the objective to develop a basic website where a consumer is provided with a shopping cart website and also to know about the technologies used to develop such a website.
This document will discuss each of the underlying technologies to create and implement an e- commerce website.
Hybrid optimization of pumped hydro system and solar- Engr. Abdul-Azeez.pdffxintegritypublishin
Advancements in technology unveil a myriad of electrical and electronic breakthroughs geared towards efficiently harnessing limited resources to meet human energy demands. The optimization of hybrid solar PV panels and pumped hydro energy supply systems plays a pivotal role in utilizing natural resources effectively. This initiative not only benefits humanity but also fosters environmental sustainability. The study investigated the design optimization of these hybrid systems, focusing on understanding solar radiation patterns, identifying geographical influences on solar radiation, formulating a mathematical model for system optimization, and determining the optimal configuration of PV panels and pumped hydro storage. Through a comparative analysis approach and eight weeks of data collection, the study addressed key research questions related to solar radiation patterns and optimal system design. The findings highlighted regions with heightened solar radiation levels, showcasing substantial potential for power generation and emphasizing the system's efficiency. Optimizing system design significantly boosted power generation, promoted renewable energy utilization, and enhanced energy storage capacity. The study underscored the benefits of optimizing hybrid solar PV panels and pumped hydro energy supply systems for sustainable energy usage. Optimizing the design of solar PV panels and pumped hydro energy supply systems as examined across diverse climatic conditions in a developing country, not only enhances power generation but also improves the integration of renewable energy sources and boosts energy storage capacities, particularly beneficial for less economically prosperous regions. Additionally, the study provides valuable insights for advancing energy research in economically viable areas. Recommendations included conducting site-specific assessments, utilizing advanced modeling tools, implementing regular maintenance protocols, and enhancing communication among system components.
Immunizing Image Classifiers Against Localized Adversary Attacksgerogepatton
This paper addresses the vulnerability of deep learning models, particularly convolutional neural networks
(CNN)s, to adversarial attacks and presents a proactive training technique designed to counter them. We
introduce a novel volumization algorithm, which transforms 2D images into 3D volumetric representations.
When combined with 3D convolution and deep curriculum learning optimization (CLO), itsignificantly improves
the immunity of models against localized universal attacks by up to 40%. We evaluate our proposed approach
using contemporary CNN architectures and the modified Canadian Institute for Advanced Research (CIFAR-10
and CIFAR-100) and ImageNet Large Scale Visual Recognition Challenge (ILSVRC12) datasets, showcasing
accuracy improvements over previous techniques. The results indicate that the combination of the volumetric
input and curriculum learning holds significant promise for mitigating adversarial attacks without necessitating
adversary training.
Industrial Training at Shahjalal Fertilizer Company Limited (SFCL)MdTanvirMahtab2
This presentation is about the working procedure of Shahjalal Fertilizer Company Limited (SFCL). A Govt. owned Company of Bangladesh Chemical Industries Corporation under Ministry of Industries.
TECHNICAL TRAINING MANUAL GENERAL FAMILIARIZATION COURSEDuvanRamosGarzon1
AIRCRAFT GENERAL
The Single Aisle is the most advanced family aircraft in service today, with fly-by-wire flight controls.
The A318, A319, A320 and A321 are twin-engine subsonic medium range aircraft.
The family offers a choice of engines
About
Indigenized remote control interface card suitable for MAFI system CCR equipment. Compatible for IDM8000 CCR. Backplane mounted serial and TCP/Ethernet communication module for CCR remote access. IDM 8000 CCR remote control on serial and TCP protocol.
• Remote control: Parallel or serial interface.
• Compatible with MAFI CCR system.
• Compatible with IDM8000 CCR.
• Compatible with Backplane mount serial communication.
• Compatible with commercial and Defence aviation CCR system.
• Remote control system for accessing CCR and allied system over serial or TCP.
• Indigenized local Support/presence in India.
• Easy in configuration using DIP switches.
Technical Specifications
Indigenized remote control interface card suitable for MAFI system CCR equipment. Compatible for IDM8000 CCR. Backplane mounted serial and TCP/Ethernet communication module for CCR remote access. IDM 8000 CCR remote control on serial and TCP protocol.
Key Features
Indigenized remote control interface card suitable for MAFI system CCR equipment. Compatible for IDM8000 CCR. Backplane mounted serial and TCP/Ethernet communication module for CCR remote access. IDM 8000 CCR remote control on serial and TCP protocol.
• Remote control: Parallel or serial interface
• Compatible with MAFI CCR system
• Copatiable with IDM8000 CCR
• Compatible with Backplane mount serial communication.
• Compatible with commercial and Defence aviation CCR system.
• Remote control system for accessing CCR and allied system over serial or TCP.
• Indigenized local Support/presence in India.
Application
• Remote control: Parallel or serial interface.
• Compatible with MAFI CCR system.
• Compatible with IDM8000 CCR.
• Compatible with Backplane mount serial communication.
• Compatible with commercial and Defence aviation CCR system.
• Remote control system for accessing CCR and allied system over serial or TCP.
• Indigenized local Support/presence in India.
• Easy in configuration using DIP switches.
Cosmetic shop management system project report.pdfKamal Acharya
Buying new cosmetic products is difficult. It can even be scary for those who have sensitive skin and are prone to skin trouble. The information needed to alleviate this problem is on the back of each product, but it's thought to interpret those ingredient lists unless you have a background in chemistry.
Instead of buying and hoping for the best, we can use data science to help us predict which products may be good fits for us. It includes various function programs to do the above mentioned tasks.
Data file handling has been effectively used in the program.
The automated cosmetic shop management system should deal with the automation of general workflow and administration process of the shop. The main processes of the system focus on customer's request where the system is able to search the most appropriate products and deliver it to the customers. It should help the employees to quickly identify the list of cosmetic product that have reached the minimum quantity and also keep a track of expired date for each cosmetic product. It should help the employees to find the rack number in which the product is placed.It is also Faster and more efficient way.
Event Management System Vb Net Project Report.pdfKamal Acharya
In present era, the scopes of information technology growing with a very fast .We do not see any are untouched from this industry. The scope of information technology has become wider includes: Business and industry. Household Business, Communication, Education, Entertainment, Science, Medicine, Engineering, Distance Learning, Weather Forecasting. Carrier Searching and so on.
My project named “Event Management System” is software that store and maintained all events coordinated in college. It also helpful to print related reports. My project will help to record the events coordinated by faculties with their Name, Event subject, date & details in an efficient & effective ways.
In my system we have to make a system by which a user can record all events coordinated by a particular faculty. In our proposed system some more featured are added which differs it from the existing system such as security.
Water scarcity is the lack of fresh water resources to meet the standard water demand. There are two type of water scarcity. One is physical. The other is economic water scarcity.
NO1 Uk best vashikaran specialist in delhi vashikaran baba near me online vas...Amil Baba Dawood bangali
Contact with Dawood Bhai Just call on +92322-6382012 and we'll help you. We'll solve all your problems within 12 to 24 hours and with 101% guarantee and with astrology systematic. If you want to take any personal or professional advice then also you can call us on +92322-6382012 , ONLINE LOVE PROBLEM & Other all types of Daily Life Problem's.Then CALL or WHATSAPP us on +92322-6382012 and Get all these problems solutions here by Amil Baba DAWOOD BANGALI
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Overview of the fundamental roles in Hydropower generation and the components involved in wider Electrical Engineering.
This paper presents the design and construction of hydroelectric dams from the hydrologist’s survey of the valley before construction, all aspects and involved disciplines, fluid dynamics, structural engineering, generation and mains frequency regulation to the very transmission of power through the network in the United Kingdom.
Author: Robbie Edward Sayers
Collaborators and co editors: Charlie Sims and Connor Healey.
(C) 2024 Robbie E. Sayers
Anti-Adhesion and Anti-Biofilm Effectiveness of Disinfectants Used In Hemodialysis against both Staphylococcus Warneri and Staphylococcus Sciuri Biofilms
1. Ghazlane Zineb et al Int. Journal of Engineering Research and Applications www.ijera.com
ISSN : 2248-9622, Vol. 4, Issue 7( Version 2), July 2014, pp.86-92
www.ijera.com 86 | P a g e
Anti-Adhesion and Anti-Biofilm Effectiveness of Disinfectants Used In Hemodialysis against both Staphylococcus Warneri and Staphylococcus Sciuri Biofilms Ghazlane Zineb1,2, Mliji El Mostafa1,3,Ghanmi Youssef1, Kerouani Abdellatif1, Oufrid Salwa1,Houari Abdellah2, K. Ibnsouda Saad2,Elmdaghri Naima1,3, Timinouni Mohammed1 1Laboratory of Molecular Bacteriology, Pasteur Institute, Casablanca, Morocco. 2Laboratory of Microbial Biotechnology, Faculty of Sciences and Techniques, Fez, Morocco. 3Laboratory of environnementale security, Pasteur Institute, Casablanca, Morocco. ABSTRACT Biofilms are communities of microorganisms attached to a surface and included in an extracellular matrix making it resistant to exogenous deleterious agents. The aim of this study is to evaluate the anti-adhesive and anti-biofilm effect of five commercials disinfectants having different active principles (hydrogen peroxide, sodium hypochlorite, isopropyl alcohol and ethanol) on four Staphylococcus strains isolated from hemodialysis unit surfaces. The disinfectants anti-adhesive effect was estimated to an exceeding rate 70% for the various studied dilutions and 90% towards the pure products. Whereas the anti-biofilm effect showed an elimination rate varying between 10 % and 95 % according to the following parameters: active principle, time of contact, concentration and bacterial strain. Our study demonstrated that all tested products have an interesting anti- adhesive effect and that the peroxide of hydrogen is endowed with important anti-biofilm efficiency, followed by the alcoholic products and the sodium hypochlorite.
Keywords - anti-adhesive effect, anti-biofilm effect, disinfectants, Staphylococcus warneri, Staphylococcus sciuri
I. INTRODUCTION
Bacterial infections in hemodialysis have two origins related or unrelated to vascular access [1]. During their stay in hemodialysis services, the inert surfaces may represent the sites of microorganisms adhesion forming stronger biofilms resistant to antimicrobial agents [2]. Staphylococcus warneri and Staphylococcus sciuri, such as other Gram positive bacteria, produces structured aggregates called biofilms, protected by a matrix composed primarily of complex polysaccharides [3]. These biofilms form a physical barrier against the entry of antimicrobial agents, and are considered pathognomic of chronic infections among attained patients [4]. Indeed, the infection is a major cause of morbidity and mortality in patient with renal failure dialyzed, and is responsible for around 15% of deaths according to the National Institute of Diabetes and Digestive and Kidney Diseases. All of these elements highlight the importance of studying the effectiveness of disinfectants used in the hemodialysis service against biofilms. Our work join within this framework, whose main objective is to evaluate the anti-biofilm and anti-adhesion potential of five disinfectants.
II. MATERIALS AND METHODS
2.1 Strains tests
The antibacterial activity was evaluated on four strains isolated from hemodialysis unit surfaces (three Staphylococcus warneri “3, 17, 20” and one Staphylococcus sciuri “9”). All strains were revived from glycerol stock cultures kept at -80°C and sub- cultured onto lysogeny broth (LB) agar plates and incubated at 37°C for 24 h. Prior to use in the adherence and biofilm experiments, the cells were harvested, washed twice in 0,1 M (KNO3) and adjusted to 107-108 CFU/ml.
2.2 Products tests
In this study, the antimicrobial activity was investigated for five commercial disinfectants having different active principles summarized in Table 1. The anti-adhesive and anti-biofilm effect of differents commercial disinfectants was tested on polystyrene flat-bottomed microtitre plates.
RESEARCH ARTICLE OPEN ACCESS
2. Ghazlane Zineb et al Int. Journal of Engineering Research and Applications www.ijera.com
ISSN : 2248-9622, Vol. 4, Issue 7( Version 2), July 2014, pp.86-92
www.ijera.com 87 | P a g e
Table 1: The active principles of five tested disinfectants. P: Product.
Products
active principles
pH
P1
H202 (2%)
2-3
P2
Ethanol (94 %),1-propanol, wetting agents
6
P3
H202 (50%) stabilized by argent
1,7
P4
isopropyl alcohol, cationic surfactants, alkaline complex
11,5- 12,5
P5
sodium hypochlorite (12°)
11,5
2.3 Determination of MIC
The Minimum Inhibitory Concentration of disinfectants studied on planktonic cells was determined using a broth dilution micro-method on polystyrene flatbottomed microtiter plates previously described by National committee for clinical Laboratory Standards [5]. The data from at least three replicates were evaluated and modal results were calculated.
2.4 Prevention Protocol
The prevention protocol was performed according to Leroy C. [6] with the following modifications; this protocol consist to add 200 μl of the product at each concentration tested (Table 3) with bacterial suspenssion per well and incubated for 8 hours to 37° C. After incubation, the biofilm is revealed with crystal violet as described in the paragraph 6.
2.5 Washing Protocol
The washing protocol was performed according to Leroy C. [6] with the following modifications; this protocol involves depositing 220 μl of the product at each concentration tested (Table 3) per well on biofilm preformed by 8 hours. After incubation to 10, 30 and 60 min, the biofilm is revealed with crystal violet as described in the paragraph 6. Indeed, the biofilm is preformed by incubating a bacterial suspension distributed per well of a sterile 96-well microplate. After 8 hours of incubation, plates were washed three times with sterile distilled water to remove any loosely associated or planktonic bacteria.
2.6 Crystal Violet staining assay
Biofilm formation was indirectly assessed using the modified crystal violet assay as described previously [7]. In brief, after the incubation period, plates were washed three times with sterile distilled water to remove any loosely associated or planktonic bacteria. The plates were air-dried. The wells were then stained with 220 μl of 1% crystal violet and incubated at room temperature for 15 min following by three times wash with sterile distilled water. The semiquantitative assessment of biofilm formation was performed by adding 220 μl of ethanol to destain the wells. 220 μl from each well was then transferred to a new plate and the absorbance determined at 550 nm. Sterility check and biofilm positive control were performed for each strain. The mean of the triplicate samples and the standard deviations were determined and plotted against EOC incubation time. The antimicrobial effect was measured by comparing the readings of the EOC treated biofilms to a positive and negative control.
2.7 Analysis Method
The micro titer screening method was used to quantitatively measure the removal efficacy of commercial disinfectants on biofilms of Staphylococcus. A measure of efficacy called Percentage Reduction (Percentage Removal) was used to evaluate the efficacy of five disinfectants. Percentage Reduction (Percentage Removal) = [(C – B) – (T – B))/ (C – B)] × 100% Where: B denotes, the average absorbance per well for blank (no biofilm, no treatment); C denotes the average absorbance per well for control wells (biofilm, no treatment) and T denotes the average absorbance per well for treated wells (biofilm and treatment).
III. RESULTS
3.1 MIC: Efficiency threshold of five commercial disinfectants
The MICs of disinfectants studied on planktonic cells were summarized in Table 2. These results showed that each test product has a specific action that varies depending on the microorganisms. Note that both products P3 and P4 have the same MIC, and it is the lowest MIC, hence their high efficiencies on bacteria in suspension. Founding to the determined MICs, six dilutions were selected with increasing concentrations, to test the anti-adhesive and anti-biofilm disinfectants effect. Table 3 focuses on these dilutions.
3.2 Anti-adhesion effect
The prevention protocol was used to determine the activity of disinfectants on adherence ability of Staphylococcus warneri "3, 17, 20" and Staphylococcus sciuri "9". The disinfectants anti- adhesive effect was estimated to an exceeding rate 70% for the various studied dilutions and 90% towards the pure products. Product P1, P2 and P3 showed a highest percentage reduction (Fig.1). While both product P3 and P4 were analyzed by the same concentrations but the results showed that the product P3 present a higher anti-adhesive effect. Where P5 remains less powerful than P3 (Fig.1).
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3.3 Anti-biofilm effect
Bacterial strains were exposed to the different tested disinfectants listed in Table 1 at different concentrations (Table 3) in triplicate, at three different time exposures (10, 30 and 60 minutes). The anti-biofilm effect showed an elimination rate varying between 10 % and 95 %, this removal percentage of biofilm increases by rising the time of treatment (Fig. 2). The hydrogen peroxide present a highest anti-biofilm effect than other products. This was followed by alcoholic products and the sodium hypochlorite (Fig. 2). However, the active principle concentration in the product P3 is raised than product P1, this highest concentration increases its anti- biofilm effect. This ascertainment was noted even in raising the concentration for all tested products (Fig. 2). Table 2: Minimum Inhibitory Concentrations of the products tested on four strains (Staphylococcus warneri “3, 17, 20” and one Staphylococcus sciuri “9”). P: Product
Products
Strain 3
Strain 9
Strain17
Strain 20
P1
1/40
1/40
1/40
1/80
P2
1/10
1/20
1/40
1/10
P3
1/640
1/640
1/640
1/640
P4
1/640
1/640
1/640
1/640
P5
1/16
1/16
1/32
1/80
Table 3: Dilutions used for testing the anti-adhesion and anti-biofilm effects of the five disinfections tested. P: product.
Products
Dilution 1
Dilution 2
Dilution 3
Dilution 4
Dilution 5
Dilution 6
P1
1/40
1/16
1/8
1/4
1/2
Pure
P2
1/10
1/8
1/6
1/4
1/2
Pure
P3
1/640
1/64
1/16
1/4
1/2
Pure
P4
1/640
1/64
1/16
1/4
1/2
Pure
P5
1/32
1/16
1/8
1/4
1/2
Pure
Figure 1: the anti-adhesion efficacy of five disinfectants, in four strains (Staphylococcus warneri “3, 17, 20” and Staphylococcus sciuri “9”), expressed as reduction percentage. P: product.
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(a) (b)
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(c) Figure 2: anti-biofilm effect of the five disinfectants tested, in four strains (Staphylococcus warneri “3, 17, 20” and Staphylococcus sciuri “9”), expressed as removal percentage after three times of treatment, (a): 10 min, (b): 30 min, (c): 60 min. P: product.
IV. DISCUSSION
Recently, scientific interest in the anti-biofilm properties of disinfectants has increased remarkably [4, 8, 9]. In this study, we initially assessed the efficacy of five disinfectants on inhibiting the growth of planktonic strains. The MICs results show obviously that the four strains are sensitive to the tested products. For comparison, we note that both products P1 and P3 had the same active ingredient (hydrogen peroxide), while their action is different, for the simple reason of their different concentration of H202. In addition, the P3 is stabilized by argent, which explains its bactericidal effect [10]. Thus efficacy of the product P4 is mainly due to one of its active ingredient which is isopropyl alcohol; the most widely used and known to be effective against bacteria [11, 12]. According to the literature, alcoholic compounds act by denaturing proteins, such as solvents or dehydrating agents [13]. In conclusion, the sensitivity of tested strains towards the five products that explains their effectiveness on bacteria in suspension.
Concerning the anti-adhesive effect, excellent activity was marked against the four strains studied. This efficiency could not reach 100%, this can be explained by the presence of proteins, whether in the LB culture medium or in the matrix protecting biofilm, decreasing their anti-adhesive effect [3, 14]. While the results of the anti-biofilm effect show that the removal rate is somewhat important. This effect depends on four parameters: (i) the active principle, (ii) the concentration of the product, (iii) the contact time (iiii) and the tested strains. We found that the product P3 containing hydrogen peroxide (H2O2) has a substantial elimination percentage versus to alcohol and chlorine (sodium hypochlorite) products. The anti-biofilm effect of H2O2 reside in its ability to pass through the biofilm and generate free radicals degrading the polysaccharides that constitute a barrier to protect the bacteria against biocides degrading. While the effectiveness of the product P3 is accentuated by the addition of argent, in addition to its antimicrobial and anti-adhesive properties. Moreover, the chlorine- based product present a lower removal biofilm percentage than H2O2. Indeed, several studies have shown that the anti-biofilm effect of chlorine is important on young biofilms aged some hours, which explains our results since the study was conducted on a mature biofilm. In addition, we can add that the concentration 38 mg/l of chlorine corresponding to 12 ° of bleach was not sufficient to remove all of the biofilm [8, 15].
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Alcohol families studied showed that the isopropyl alcohol has a low MIC relative to the ethanol product that means its important effect on planktonic bacteria. However, the anti-biofilm effect noted a greater important with isopropyl alcohol, however it is lower than the hydrogen peroxide products. A study was performed on Staphylococcus epidermidis biofilm, showed that the ethanol wash is responsible for the induction of biofilm formation [16]. The biofilm elimination rate which reaches not 100% can be explained by the resistance to disinfectants. Several mechanisms have been given. Some studies have pointed to mechanisms involving in particular that the barrier formed by the biofilm, share of its organic consistency, it prevents antimicrobials or antibiotics access by limiting their diffusion or their repulsion [17]. This may be due to electrostatic repulsion or sequestration by the surface polymers [18]. Other studies have suggested that this resistance causes a slow or incomplete penetration of disinfectants to biofilm. Furthermore, the presence of a neutralizing disinfectant microenvironment [12, 19, 20] or by inhibition of certain active principles such as the inhibition of oxidants by the presence of proteins, inducing poor diffusion of the product within the biofilm [21].
V. CONCLUSION
Bacterial biofilm communities present a tank of virulence and interbacterienne transmission genes resistance. Their presence in hospitals especially in hemodialysis services represents a major problem of public health. Despite the antibiotic sensitivity possibility of strains studied, their ability to form biofilms makes them susceptible to acquire resistance genes. This is evident with intercellular communication called quorum sensing. Indeed, an adequate and consistent control of products used for cleaning and disinfecting is required. In addition, periodic assessment of effectiveness or resistance carries a major interest. Ensuring thus, the reduction of patient morbidity and number of hospitalizations and improved quality of life.
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