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Napier grass (Cenchrus purpureus Schumach L.) is an important perennial forage native to Africa and now introduced and grown in many tropical and
subtropical countries. It is considered as a short-term drought tolerant forage, which is a useful trait in areas with low soil moisture during the dry season,
although it is recommended for planting in areas with rainfall >1,000 mm. In order to exploit the potential of this grass species for improved water use
efficiency (WUE), a field drought stress experiment was conducted with the objective to identify traits that underlie enhanced water use efficiency and to
select best performing genotypes that can thrive in low soil moisture areas.
Identification of Water Use Efficient Napier Grass Accessions
Using Field Drought Stress
▪ Eighty four accessions from ILRI and EMBRAPA
collections of Napier grass were planted using a P-rep
design in four blocks in Bishoftu, Ethiopia.
▪ After establishment plants were exposed to
optimum water (OW) with 20% soil moisture or
water stress (WS) with 10% soil moisture during the
dry season.
▪ Agronomic and physiological data were collected at
every 8 weeks of re-growth.
METHODOLOGY
Fig. 1. Napier grass growing under optimum water (OW, left panel) and water stress (WS, right panel) conditions
▪ The study revealed significant differences between plants grown under optimum water and
water stress (Fig. 1).
▪ Significant genotypic differences were observed among genotypes for morphological and
agronomic traits which suggests selection for improved forage performance will be efficient
(Table 1.).
▪ Genotypes showed significant variation for total dry weight (Fig. 2) and enhanced water use
efficiency (Fig. 3) implying that genotypes differ in economic use of water for increased
biomass production under water limited conditions.
▪ Genotypes showed consistent performance for biomass dry weight and water use efficiency
observed across dry season harvests, indicating promising Napier grass accessions could be
identified for low soil moisture areas forage production.
INTRODUCTION
0
0.5
1
1.5
2
2.5
3
3.5
4
4.5
1026
14355
14389
14982
14983
14984
15357
15743(MOTT)
16621
16782
16783
16784
16785
16786
16787
16788
16789
16790
16791
16792
16793
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18448
18662
BAGCE100
BAGCE17
BAGCE30
BAGCE343
BAGCE53
BAGCE81
BAGCE86
BAGCE90
BAGCE97
CNPGL00-1-1
CNPGL92-133-3
CNPGL92-198-7
CNPGL92-56-2
CNPGL92-66-3
CNPGL9279-2
CNPGL93-37-5
CNPGL93-01-1
CNPGL93-04-2
CNPGL93-18-2
CNPGL94-13-1
CNPGL96-21-1
CNPGL96-23-1
CNPGL96-27-3
PIONEIRO
TDW(t/ha)
OW WS
0
1
2
3
4
5
6
7
8
1026
143551438914982
14983
14984
15357
15743 (MOTT)
16621
16782
16783
16784
16785
16786
16787
16788
16789
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16791
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16839
16840
16902
18438
18448
18662
BAGCE 100
BAGCE 17
BAGCE 30
BAGCE 343
BAGCE 53
BAGCE 81
BAGCE 86
BAGCE 90
BAGCE 97
CNPGL 00-1-1
CNPGL 92-…
CNPGL 92-…
CNPGL 92-56-2
CNPGL 92-66-3
CNPGL 9279-2
CNPGL 93 -37-5
CNPGL 93-01-1
CNPGL 93-04-2
CNPGL 93-18-2
CNPGL 94-13-1
CNPGL 96-21-1
CNPGL 96-23-1CNPGL 96-27-3PIONEIRO
WUE g/m3
OW WS
Fig. 2. Total dry weight of 84 Napier grass accessions averaged over four dry season harvests between OW/WS
Table 1. Summary ANOVA and coefficient of variation
for morphological and agronomic traits of four dry season harvests
Fig. 3. WUE of 84 Napier genotypes under OW/WS over four dry
season harvests
Sources of
Variation PH LW TN TFW TDW WUE
Genotype 139.39*** 151.79*** 15293.4*** 51.08*** 4.74*** 25.26***
Treatment
(OW/WS) 3306.68** 857.3* 33433*** 3377.94** 308.73** 109.3**
Genotype X
Treatment 21.07* 19.69ns 3287.9*** 18.36*** 1.72* 7.93*
Range 1.67-60.33 3-35.33 0.67-264 0.02-36.74 0.04-17.42 0.05-27.24
Mean 12.11 19.01 82.52 3.89 1.2 2.75
GCV% 31.26 21.57 51.37 58.29 54.39 55.07
PCV% 51.64 30.74 63.14 69.84 71.49 72.35
PH = plant height; LW = Leaf width; LL = Leaf length; TFW = Total fresh weight; TDW = Total dry weight
WUE =Water use efficiency; *,**,*** = Significant level at 0.5% .0.1%and 0.001%, ns=Non significant
MAJOR FINDINGS
Ermias Habte:
E.Habte@cgiar.org, Feed and Forage Development, International
Livestock Research Institute (ILRI), Addis Ababa, Ethiopia. ilri.org
This research was supported by the "Cooperative Research Program for Agriculture Science
& Technology Development (Project No. PJ012187)" , Rural Development Administration,
Republic of Korea
Ermias Habte, Meki S. Muktar, Alemayehu T. Negawo, Ki-Won Lee and Chris S. Jones
RESULTS
This document is licensed for use under the Creative Commons Attribution 4.0 International Licence.
Date Year. July,2019

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Identification of water use efficient Napier grass accessions using field drought stress

  • 1. Napier grass (Cenchrus purpureus Schumach L.) is an important perennial forage native to Africa and now introduced and grown in many tropical and subtropical countries. It is considered as a short-term drought tolerant forage, which is a useful trait in areas with low soil moisture during the dry season, although it is recommended for planting in areas with rainfall >1,000 mm. In order to exploit the potential of this grass species for improved water use efficiency (WUE), a field drought stress experiment was conducted with the objective to identify traits that underlie enhanced water use efficiency and to select best performing genotypes that can thrive in low soil moisture areas. Identification of Water Use Efficient Napier Grass Accessions Using Field Drought Stress ▪ Eighty four accessions from ILRI and EMBRAPA collections of Napier grass were planted using a P-rep design in four blocks in Bishoftu, Ethiopia. ▪ After establishment plants were exposed to optimum water (OW) with 20% soil moisture or water stress (WS) with 10% soil moisture during the dry season. ▪ Agronomic and physiological data were collected at every 8 weeks of re-growth. METHODOLOGY Fig. 1. Napier grass growing under optimum water (OW, left panel) and water stress (WS, right panel) conditions ▪ The study revealed significant differences between plants grown under optimum water and water stress (Fig. 1). ▪ Significant genotypic differences were observed among genotypes for morphological and agronomic traits which suggests selection for improved forage performance will be efficient (Table 1.). ▪ Genotypes showed significant variation for total dry weight (Fig. 2) and enhanced water use efficiency (Fig. 3) implying that genotypes differ in economic use of water for increased biomass production under water limited conditions. ▪ Genotypes showed consistent performance for biomass dry weight and water use efficiency observed across dry season harvests, indicating promising Napier grass accessions could be identified for low soil moisture areas forage production. INTRODUCTION 0 0.5 1 1.5 2 2.5 3 3.5 4 4.5 1026 14355 14389 14982 14983 14984 15357 15743(MOTT) 16621 16782 16783 16784 16785 16786 16787 16788 16789 16790 16791 16792 16793 16794 16795 16796 16797 16798 16799 16800 16801 16802 16803 16804 16805 16806 16807 16808 16809 16810 16811 16812 16813 16814 16815 16816 16817 16818 16819 16821 16822 16834 16835 16836 16837 16838 16839 16840 16902 18438 18448 18662 BAGCE100 BAGCE17 BAGCE30 BAGCE343 BAGCE53 BAGCE81 BAGCE86 BAGCE90 BAGCE97 CNPGL00-1-1 CNPGL92-133-3 CNPGL92-198-7 CNPGL92-56-2 CNPGL92-66-3 CNPGL9279-2 CNPGL93-37-5 CNPGL93-01-1 CNPGL93-04-2 CNPGL93-18-2 CNPGL94-13-1 CNPGL96-21-1 CNPGL96-23-1 CNPGL96-27-3 PIONEIRO TDW(t/ha) OW WS 0 1 2 3 4 5 6 7 8 1026 143551438914982 14983 14984 15357 15743 (MOTT) 16621 16782 16783 16784 16785 16786 16787 16788 16789 16790 16791 16792 16793 16794 16795 16796 16797 16798 16799 16800 16801 16802 16803 16804 16805 16806 16807 16808 16809 16810 16811 168121681316814 16815 168161681716818 16819 16821 16822 16834 16835 16836 16837 16838 16839 16840 16902 18438 18448 18662 BAGCE 100 BAGCE 17 BAGCE 30 BAGCE 343 BAGCE 53 BAGCE 81 BAGCE 86 BAGCE 90 BAGCE 97 CNPGL 00-1-1 CNPGL 92-… CNPGL 92-… CNPGL 92-56-2 CNPGL 92-66-3 CNPGL 9279-2 CNPGL 93 -37-5 CNPGL 93-01-1 CNPGL 93-04-2 CNPGL 93-18-2 CNPGL 94-13-1 CNPGL 96-21-1 CNPGL 96-23-1CNPGL 96-27-3PIONEIRO WUE g/m3 OW WS Fig. 2. Total dry weight of 84 Napier grass accessions averaged over four dry season harvests between OW/WS Table 1. Summary ANOVA and coefficient of variation for morphological and agronomic traits of four dry season harvests Fig. 3. WUE of 84 Napier genotypes under OW/WS over four dry season harvests Sources of Variation PH LW TN TFW TDW WUE Genotype 139.39*** 151.79*** 15293.4*** 51.08*** 4.74*** 25.26*** Treatment (OW/WS) 3306.68** 857.3* 33433*** 3377.94** 308.73** 109.3** Genotype X Treatment 21.07* 19.69ns 3287.9*** 18.36*** 1.72* 7.93* Range 1.67-60.33 3-35.33 0.67-264 0.02-36.74 0.04-17.42 0.05-27.24 Mean 12.11 19.01 82.52 3.89 1.2 2.75 GCV% 31.26 21.57 51.37 58.29 54.39 55.07 PCV% 51.64 30.74 63.14 69.84 71.49 72.35 PH = plant height; LW = Leaf width; LL = Leaf length; TFW = Total fresh weight; TDW = Total dry weight WUE =Water use efficiency; *,**,*** = Significant level at 0.5% .0.1%and 0.001%, ns=Non significant MAJOR FINDINGS Ermias Habte: E.Habte@cgiar.org, Feed and Forage Development, International Livestock Research Institute (ILRI), Addis Ababa, Ethiopia. ilri.org This research was supported by the "Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ012187)" , Rural Development Administration, Republic of Korea Ermias Habte, Meki S. Muktar, Alemayehu T. Negawo, Ki-Won Lee and Chris S. Jones RESULTS This document is licensed for use under the Creative Commons Attribution 4.0 International Licence. Date Year. July,2019