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Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
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Slide 5: Plasmid Inheritance
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Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
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One Health in action: Perspectives from 10 years in the field
1. Better lives through livestock
One Health in action: Perspectives
from 10 years in the field
Shauna Richards1 and Anselme Shyaka2
1Postdoctoral fellow, Animal and Human Health program, International Livestock Research Institute
2University of Global Health Equity, Rwanda
Virtual seminar at Acadia University, Wolfville Nova Scotia, Canada, 26 October 2023
3. 3
ILRI is co-hosted by both the
governments of Ethiopia and Kenya,
with offices in 8 other countries in
Africa (Burkina Faso, Burundi, Mali,
Nigeria, Senegal, Tanzania, Uganda
and Zimbabwe); 4 countries in Asia
(China, India, Nepal and Vietnam).
ILRI has approximately 600
permanent staff (with a gender
breakdown of 40% female and 60%
male).
ILRI offices and
staff worldwide
6. 6
COHESA Overall Objective
The project aims to generate an inclusive
Research & Innovation ecosystem,
facilitating rapid uptake, adaption and
adoption of solutions to One Health (OH)
issues, with the OH concept embedded
across society in Eastern and Southern
Africa (ESA), working for healthy humans,
animals and environments using a systems
approach to learning.
7. 7
Duration 48 Months –
December 2021 – 2025
Countries
EA Ethiopia, Kenya,
Rwanda, Somalia, Tanzania,
Uganda
SA Botswana, Namibia,
Malawi, Mozambique,
Zambia, Zimbabwe
Bold Deep Dive, Standard
Italics, rest are focussed
Consortium
ILRI – International Livestock
Research Institute
CIRAD – French Agricultural
Research Centre for
International Development
ISAAA AfriCentre –
International Service for the
Acquisition of Agri-biotech
Applications
University of Pretoria assist
as partner
COHESA Consortium and Countries
8. 8
Donor
This project is supported by the European Commission OACPS Research and
Innovation Programme: ACP-EU initiative, co-founded through ILRI and CIRAD
9. 9
o Increased relevance of OH research and policies in Eastern
and Southern Africa (ESA)
o Enhanced national and subregional cross-sectoral
collaboration between government entities with OH
mandates and OH stakeholders across society
o Educational and research institutes equipped to train the
next generation workforce in tackling OH issues
o Increased capacity of government and non-governmental
stakeholders to identify and deliver OH solutions to final
beneficiaries
Objectives
10. 10
Work Packages
WP1 - Understanding OH
capacity, knowledge and
information sharing
- All countries
WP2 - Promoting
national and regional OH
collaboration and
governance
- All countries
WP3 - Building the future
OH workforce
- Deep Dive, limited in
Standard and Focussed
programme
WP4 - Delivering OH
solutions
Deep Dive, little in
Standard and Focussed
programme
WP5 – Project
management
Consortium led by ILRI,
CIRAD, ISAAA
12. 12
BOTSWANA ETHIOPIA KENYA MOZAMBIQUE NAMIBIA RWANDA
TANZANIA
MALAWI UGANDA
ZAMBIA ZIMBABWE SOUTH AFRICA
(partner)
Project Design – Led by Consortium & Delivered by Multipliers
Dr. Yusuf Harad
SOMALIA
13. 13
Baseline: To perform a baseline & endline assessments with key
informants on One Health topics on the following:
1. One Health national challenges, gaps, and capacities
2. Understand current One Health Research and Innovation
landscape to identify strengths and weaknesses
3. Identify rapid approaches to identify key One Health issues
4. Assess sectoral performance in One Health, capacity, and
bottlenecks
5. Avail baseline and endline data to evaluate performance
and impact of COHESA project
Online Observatory & Net-mapping
Work Package 1 – One Health Status
14. 14
o Promote National and regional OH collaboration
and governance
o Training and learning support government entities on
OH collaboration
o Strengthen the operation of existing or new OH
platforms that involve cross government collaboration
o Training and learning support to government entities
on addressing gaps and weaknesses in existing OH
goals, strategies, action plans, and policies
Work Package 2 – One Health Governance
15. 15
Work Package 3 – Future OH Workforce
One Health
outreach
One Health
capacity
building
material
Bench-
marking
curricula
Baseline
survey on
One Health
education
needs
16. 16
Capacitate Research Institutes to identify, develop,
adapt, and deliver OH solutions for OH focal topics
o Develop and submit research proposal
o Review OH adaption and delivery mechanisms
related to focal topic
Work Package 4 – One Health Implementation
17. 17
Summary
- One Health needed to face the major challenges of our time
- COHESA seeks to capacitate countries to face these challenges
- 12 countries in Eastern and Southern Africa - 4 years – 2022-25
- Consortium delivers project through in-country multipliers to national and local
stakeholders
i.e. Government, Education, Research, Service providers
- Build on and collaborate with other One Health initiatives
22. Rwanda
• Small, landlocked, highly populated
• 26,338 Km2 (~ 10,169 sq. mi)
• 571 per Km2 (1,480 people per mi2)
• Various zoonotic diseases
• Rift Valley fever
• Anthrax
• Brucellosis
• Bovine Tuberculosis
• Cysticercosis
• Hotspot of emerging Infectious Diseases
• Ebola, Marburg, Zika, Chikungunya,
etc.
23. Timeline for the creation of Rwanda’s OH
Governance framework
RBC: Rwanda Biomedical Council, RAB: Rwanda Agriculture Board, RDB: Rwanda Development Board
2011: OH
Steering
Committee
(RBC, RAB, RDB)
1
2014: OH
Strategic Plan
(2014 – 2018)
2
2021: OH
Strategic Plan
(2021 – 2026)
3
2021: OH Policy
adopted
4
24.
25. Rwanda’s OH Policy
& Strategic Plan
(2021 – 2026)
Objective 1:
Create an
effective OH
platform
Objective 2: Put
in place a Joint
Surveillance
System
Objective 3:
Develop OH
workforce
capacity
Objective 4:
Promote applied
research at
interface
human-animal-
environment
Objective 5:
Strengthen
national
awareness
mechanisms on
OH issues
Objective 6:
Sustainable
mobilization of
resources
27. Theme II: Rwanda’s OH structure
Technical Working
Group #1
Office of the PM
Social Cluster
OH Multisectoral
Coordination
Mechanism (OH-MCM)
OH Secretariat
Technical Working
Group #2
Technical Working
Group #3
Technical Working
Group #4
Social cluster: MoH, Min. of Agric., MoE, Min. Educ., Min. of Emerg. Mangmnt
OH-MCM: DG RBC, RAB, RDB and VC - UR
28. In reality…
• MoH and RBC: Coordinates all activities
• Broken Linkages with other line Ministries and stakeholders
- Rabies: Ministry of Agriculture and Animal Resources/RAB
- AMR: Ministry of Agriculture and Animal Resources/RAB
- No clear linkage with MoE/REMA
- Broken linkages with Development Partners and Academia
• Insufficient Involvement of Other line Ministries
- Don’t feel empowered
- Feel underestimated
- RBC feels left alone with all the activities
30. Theme III: OH Governance in Diseases
Management
• A total of 1339 confirmed cases of RVF between 31
March 2022 and 31 December 2022
• 1285 (96%) were reported in cattle
• 34 (3%) in goats and 20 (1%) in sheep
• A total of 516 deaths (38.5%)
• Up to 1254 abortions in animals (all species
combined)
• No official counts for human’s cases
32. OH Governance in Diseases Management
District Command Post
(30 districts)
National Command Post
Data collected
:(Suspect cases or
confirmed )
Directives (Vaccination,
testing methods) Supplies
Community
(14,837 villages)
Local Government Officials
Public Health Experts
Veterinarians
Epidemiologists
Data managers (Hospitals)
Security Organs
33. Conclusion
1) Rwanda has adopted OH approach to handle complex challenges
2) OH Governance in Rwanda is well defined
- Policy documents establishing a working framework
- OH structure not yet fully operational
- Need to strengthen collaboration between key stakeholders
- Need to have OH education that graduate a workforce aware of OH approach
3) OH approach has been successfully tested in various zoonotic outbreaks
- Need to keep the momentum in period without outbreaks
34. 34
Questions?
All images are ILRI sourced or stock images via MS
PowerPoint unless specially referenced otherwise