Enzyme labeling is a method used to chemically attach enzymes to molecules like antibodies or proteins. This allows the labeled molecules to be detected and tracked during analysis. Common enzymes used for labeling include horseradish peroxidase and alkaline phosphatase, which are conjugated to antibodies, proteins, peptides, and other biomolecules. The labeling process involves chemically crosslinking the enzyme to the target molecule. The conjugates are then purified to remove any unreacted enzyme using techniques like dialysis, gel filtration, or chromatography. Labeled molecules can be analyzed using techniques like mass spectrometry, electrophoresis, or biochemical assays.

1. Enzyme Labeling
The enzyme can be modified to contain reactive groups that react with
other pre-activated small molecules and biomolecules having chemically
reactive groups such as amines, thiols, carboxylates, hydroxyls,
aldehydes, and ketones. Enzyme labelling is a method used in biological
analysis to place chemical markers on molecules within a substance.
Molecular labels allow the detection and tracking of molecules in
substances during chemical analysis or testing. Different types of tags
can be used for this type of biomarker. When one enzyme binds
chemically to another molecule, this process is called enzyme labeling.
Below is a list of our Enzyme Labeling (include but not limited to
the following):
 Horseradish Peroxidase (HRP)-Conjugated Antibodies
 Fab-HRP Conjugates
 Half Antibody-HRP Conjugate
 Alkaline Phosphatase (ALP)-Antibodies Conjugates
 Alkaline Phosphatase (ALP)-Oligo Conjugates
 Protein-Horseradish Peroxidase (HRP) Conjugates
 Protein-Alkaline Phosphatase Conjugate (ALP)
 Horseradish Peroxidase (HRP)-Peptide Conjugates
 Alkaline Phosphatase (ALP)-Peptide Conjugates

2.  Enzyme-Biotin Labeling
 Enzyme-Nanoparticle Bioconjugates
 Sample Submission Requirement
Procedure of Enzyme Labeling
The biomolecules provided by customers should be sufficiently pure.
Please provide 1-3 mg of starting material with the necessary data for
purity assessment. After labeling of enzyme with the crosslinking
reagent, final conjugates must first be isolated from excess or unreacted
reagent by gel filtration or dialysis. In many cases, simple dialysis may
suffice to remove unreacted reagent from the reaction solution.
Additional purification techniques such as stirred cell filtration, tangential
flow filtration (TFF), and gel filtration chromatography may also be used
to either remove excess reagent or isolate and characterize the
cross-linked product. For reagents (mostly protein and other biological
molecules) that are similar in size or larger than the antibody, one must
resort to other purification techniques such as affinity chromatography,
ion-exchange chromatography, and hydrophobic interaction
chromatography. Cross-linked target molecules may then be further
characterized by biochemical or biophysical techniques. After
purification, the product may be subject to many different types of
studies including spectroscopic (MALDI-TOF, ESI, LC-MS
Fluorescence), electrophoresis, immunochemical biochemical, and
enzymatical analysis.
Why Choose BOC Sciences?
BOC Sciences is proud to provide you enzyme labeling service. Our
experts and chemists are professional at enzyme labeling to help you to
make progress in your research. Moreover, our dedicated technical
account managers will guide your project through every step of the
process and constantly keep you informed of the latest project progress.
References

3. 1. Ishikawa, E., Imagawa, M., Hashida, S., Yoshitake, S., Hamaguchi, Y., &
Ueno, T. (1983). Enzyme-labeling of antibodies and their fragments for
enzyme immunoassay and immunohistochemical staining. Journal of
Immunoassay and Immunochemistry, 4(3), 209-327.
2. Estelmann, S., Hügler, M., Eisenreich, W., Werner, K., Berg, I. A.,
Ramos-Vera, W. H., ... & Fuchs, G. (2011). Labeling and enzyme studies
of the central carbon metabolism in Metallosphaera sedula. Journal of
bacteriology, 193(5), 1191-1200.