These slides made by Miss Khunsha Fatima. This presentation will cover mainly ELISA, its Basic Principle, Steps and its related topics discussed in detail.
This power point presentation is all about ELISA its procedure and different types of ELISA. briefly all the information about ELISA is given in this Power point presentation. indirect ELISA, Direct ELISA, sandwich ELISA and Competitive ELISA. its steps and about where detection antibody is used and where capture antibody.
ABSTRACT: The ELISA technique is a simple, sensitive, rapid, reliable, and versatile assay system for the quantitation of antigens and antibodies. Because of the extreme discriminating power of antibodies to recognize an almost infinite array of antigenic structures, the application of ELISA to analyte measurement is almost unlimited. ELISAs have been developed in many configurations depending on the particular application of the assay.
In solid-phase ELISA, one of the immunoreactants (antibody or antigen) is immobilized onto a solid support (microtiter plate) by adsorption, through non-covalent interactions. The immobilized antibody is then incubated with test solution containing the analyte of interest. Following a period of incubation and washing, the bound antigen is detected, by the addition of an enzyme-conjugated antibody that binds to the remaining antigenic sites on the antigen.
Although the technique is easy to perform and quite sensitive, there are certain problems to be solved before it becomes widely usable. In the present Memorandum the technical details are given and the advantages and shortcomings of the procedure are discussed. Present applications and future prospects are reviewed.
A path breaking technology which has made it possible for us to detect HIV. ELISA is an immunological assay nowadays even used to detect food proteins & is the science behind pregnancy color test. This presentation unlocks the working of this assay.
Definition of ELISA, Immunochemical principle of ELISA, Direct, Indirect, Sandwich and Competitive ELISA, applications of ELISA in the diagnostic field, and benefits/drawbacks of ELISA.
This power point presentation is all about ELISA its procedure and different types of ELISA. briefly all the information about ELISA is given in this Power point presentation. indirect ELISA, Direct ELISA, sandwich ELISA and Competitive ELISA. its steps and about where detection antibody is used and where capture antibody.
ABSTRACT: The ELISA technique is a simple, sensitive, rapid, reliable, and versatile assay system for the quantitation of antigens and antibodies. Because of the extreme discriminating power of antibodies to recognize an almost infinite array of antigenic structures, the application of ELISA to analyte measurement is almost unlimited. ELISAs have been developed in many configurations depending on the particular application of the assay.
In solid-phase ELISA, one of the immunoreactants (antibody or antigen) is immobilized onto a solid support (microtiter plate) by adsorption, through non-covalent interactions. The immobilized antibody is then incubated with test solution containing the analyte of interest. Following a period of incubation and washing, the bound antigen is detected, by the addition of an enzyme-conjugated antibody that binds to the remaining antigenic sites on the antigen.
Although the technique is easy to perform and quite sensitive, there are certain problems to be solved before it becomes widely usable. In the present Memorandum the technical details are given and the advantages and shortcomings of the procedure are discussed. Present applications and future prospects are reviewed.
A path breaking technology which has made it possible for us to detect HIV. ELISA is an immunological assay nowadays even used to detect food proteins & is the science behind pregnancy color test. This presentation unlocks the working of this assay.
Definition of ELISA, Immunochemical principle of ELISA, Direct, Indirect, Sandwich and Competitive ELISA, applications of ELISA in the diagnostic field, and benefits/drawbacks of ELISA.
This presentation explains about the principle and procedure involved in elisa method of immunoassay, development o f elisa , application advantages and disadvantages of elisa
Test for detection of plant virus by ELISA test.pdfLOKESH R
This presentation provides an overview of the Enzyme-Linked Immunosorbent Assay (ELISA) test for the detection of plant viruses. The ELISA test is a highly sensitive and specific method for detecting viral antigens in plant tissues, seeds, and other plant materials.
The presentation covers the principles of ELISA test, including the different types of ELISA tests, such as direct, indirect, sandwich, and competitive ELISA. The steps involved in the ELISA test, including sample preparation, antibody labeling, incubation, and detection, are discussed in detail.
The advantages and limitations of the ELISA test for plant virus detection are also highlighted. The presentation includes a comparison of the ELISA test with other diagnostic methods for plant viruses, such as polymerase chain reaction (PCR) and serological assays.
Finally, the presentation provides examples of the applications of the ELISA test for plant virus detection, including its use in crop protection, disease management, and quarantine measures. The presentation concludes with a summary of the key points discussed and recommendations for the use of the ELISA test in plant virus detection.
Enzyme immunoassays (EIAs), also known as enzyme-linked immunosorbent assays (ELISAs), combine antibody binding with enzymatic detection to quantify molecules of interest.
Blog praxilabs com_2021_09_20_elisa_principleAyaFarid2
The Enzyme Linked Immunosorbent Assay (ELISA) is one of the most sensitive immunoassays available. The typical detection range for an ELISA is 0.1 to 1 fmole or 0.01 ng to 0.1 ng. It is a plate-based assay technique designed for detecting and quantifying peptides, proteins, antibodies, and hormones.
ELISA, principle and method by kk sahuKAUSHAL SAHU
What is ELISA.
Principle.
History.
Types of ELISA method.
1.Direct ELISA.
2.Indirect ELISA.
3.Sandwhich ELISA.
Conclusion.
References.
Antibodies (also known as immunoglobulins abbreviated Ig) are gamma globulin proteins that are found in blood and are used by the immune system to identify and neutralize foreign objects, such as bacteria and viruses.
Sustainable Practices in Event ManagementSindhBiotech
The following presentation is presented by our intern, Lucky Qindeel Panhwar, from Phase 1 of the ongoing SBA Internship Program.
In the era of acute pollution and threatening social and environmental conditions, the utmost importance must be given to finding and applying 'green solutions'. Because such a basis will provide both ecological and economic sustainability while ensuring good social practices.
The important is that "Sustainable practices must be in action, not just in the mind".
Launching an event successfully hinges on thoughtful planning and execution. The
Implementation of strategies that prioritize eco-friendly catering, sustainable venues, and environmentally conscious promotions, is key to effective Sustainable Event Management.
For Youtube: https://youtu.be/ybPsyld3wlM?si=h9NM3PQfoyoWZKJQ
#GreenMarketing #SustainableEventsforSustainableSociety #EcoFriendlyApproaches #GreenCatering
#KarachiGreenMarathon
Potentials of Microfluids in Life Sciences: A Lab on a ChipSindhBiotech
Today marks the death anniversary of Mr. Werner Jacobi, the pioneering figure in microfluidics. While his initial focus was on microelectronics and semiconductor technology, he carved out a distinct path for microfluidics, diverging from his previous pursuits. His visionary approach continues to shape the trajectory of microfluidics research and its diverse applications in science and technology.
This lecture is presented by our team member and volunteer, Bushra Umer, from Volunteer Batch-V, 2023.
The lecture emphasizes the adoption of the "lab-on-a-chip" approach to ensure cost-effective and real-time experiments, underscoring the importance of developing such innovative techniques in today's modern arena.
For Video: https://youtu.be/ZF-c6k-F1RI
#wernerjacobi
#biotechnology
#technology
#lifesciences
#microfluidics
How Bio-inspired Plastic Outperforms Traditional Plastic: A Comparative AnalysisSindhBiotech
This presentation is brought to you by Bushra Umer, our team member and volunteer from Batch - V, 2023.
It highlights the significance of bioplastics, which outshine traditional plastics in sustainability and performance, paving the way for a cleaner, greener, and healthier planet.
Considering the ongoing environmental crisis, there is an urgent necessity to embrace such sustainable solutions to rescue Mother Earth from environmental calamities.
#Bioplastics #SustainableLiving #GreenRevolution #WorldEarthDay
Youtube Link: https://youtu.be/LFpYs_4N_1k
Common Genetic Disorders Prevailing in PakistanSindhBiotech
This Lecture is presented by our 2k23 volunteer and Content Presenter Zargull Sadein , she is from Karachi, Pakistan, and she is covering "Common Genetic Disorders Prevailing in Pakistan".
Youtube: https://youtu.be/16scqaB1yjk
The Revolutionary Progress of Artificial Inteligence (AI) in Health CareSindhBiotech
This Lecture is presented by our 2k23 volunteer Hina Nawaz, she is from Karachi, Pakistan, and she is covering "The Revolutionary Progress of Artificial Inteligence (AI) in Health Care".
Youtube: https://youtu.be/vhJRCj5ZgJc
Nanobots: Lecture on the Artificial BloodSindhBiotech
This Lecture is presented by our volunteer Laraib Elahi, she is from Karachi, Pakistan, and she is covering "Nanobots: The Artificial Blood. "
For video: https://youtu.be/TsUgvOh6tOA
Decoding the Monkeypox Virus : From Discovery to PreventionSindhBiotech
This lecture is presented by our volunteer Sajid Ali Shah, he is from Islamabad, Pakistan, and he is covering the topic Decoding the Monkeypox Virus : From Discovery to Prevention.
For video: https://youtu.be/-RT2UvTerBc
This Lecture is presented by our volunteer Abrar Hussain, he is from Karachi Pakistan, and he is covering The escence of research.
Youtube: https://youtu.be/CB0CY70K2go
The role of CRISPR CAS-9 in the treatment of HIVSindhBiotech
This lecture is presented by our volunteer Sajid Ali Shah, he is from Islamabad, Pakistan, and he is covering The role of CRISPR CAS-9 in the treatment of HIV.
for video: https://youtu.be/c0gGdzKUACI
Developing Therapeutic Approaches For Emerging Viral DiseasesSindhBiotech
This lecture is presented by our volunteer Zargul sadein, she is from Karachi, Pakistan, and she is covering "Developing Therapeutic Approaches For Emerging Viral Diseases."
for video: https://www.youtube.com/watch?v=DcI89jpjbxc
unwinding the potentials of stem cellsSindhBiotech
This lecture is presented by our volunteer Javeria Khan, she is from Karachi, Pakistan, and she is covering "unwinding the potentials of stem cells"
for video: https://youtu.be/GDC3RKwIACY
Designer babies : A Health Wonder Or an Ethical ErrorSindhBiotech
This lecture is presented by our volunteer Laraib Elahi, she is from Karachi, Pakistan, and she is covering "Designer babies : A Health Wonder Or an Ethical Error. "
For video: https://youtu.be/YGZQGL2QHuU
Personalized Medicines - Enhancers of Life's Quality and Their Future SindhBiotech
This lecture is presented by our volunteer Bushra Umer, she is from Karachi, Pakistan, and she is covering "Personalized Medicines - Enhancers of Life's Quality and Their Future ".
For video: https://www.youtube.com/watch?v=BSrgJaBYuxg
Conceptual Understanding of Monoclonal Bodies Production via Hybirdoma Techno...SindhBiotech
This video is presented by our volunteer Mehwish Khan, she is from Karachi, Pakistan, and she is covering "Monoclonal Bodies Production via Hybirdoma Technology"
for video: https://youtu.be/NilP7HIALvY
#Hybirdoma #antibody #monoclonalantibodies #biology #physiology #health #polyclonalantibody
Microbiota and Gut-Brain Axis in HealthSindhBiotech
This lecture is presented by our volunteer Hina Nawaz, she is from Karachi, Pakistan, and she is covering "Microbiota and Gut-Brain Axis in Health"
For video: https://youtu.be/W2hfl5_FaF8
Biosensors in Environmental MonitoringSindhBiotech
This lecture is presented by our volunteer Bushra Umer, she is from Karachi, Pakistan, and she is covering "Biosensors in Environmental Monitoring"
For video: https://youtu.be/DoO2Aw7bRrk
Regulation and Integration of MetabolismSindhBiotech
This lecture is presented by our volunteer Hina Nawaz, she is from Karachi, Pakistan, and she is covering "Regulation and Integration of Metabolism"
for video: https://youtu.be/D2k-_f28TQA
Dengue Virus: Genomic Insights, Pathogenic Mechanisms, and Therapeutic Approa...SindhBiotech
This lecture is presented by our volunteer Sajid Ali Shah, he is from Islamabad, Pakistan, and he is covering Dengue Virus: Genomic Insights, Pathogenic Mechanisms, and Therapeutic Approaches
for video: https://youtu.be/whrkkKR-NSY
#denguevirus #virology #virologist #genomics #covid19 #virus #pathogen #pathology #immunology
Gene environment interaction and its impact upon on human healthSindhBiotech
This lecture is presented by our volunteer Zargull Sadein, she is from Karachi Pakistan, and she is covering Gene environment interaction and its impact upon on human health.
for video: https://youtu.be/97F1tR9jj5k
The ability to recreate computational results with minimal effort and actionable metrics provides a solid foundation for scientific research and software development. When people can replicate an analysis at the touch of a button using open-source software, open data, and methods to assess and compare proposals, it significantly eases verification of results, engagement with a diverse range of contributors, and progress. However, we have yet to fully achieve this; there are still many sociotechnical frictions.
Inspired by David Donoho's vision, this talk aims to revisit the three crucial pillars of frictionless reproducibility (data sharing, code sharing, and competitive challenges) with the perspective of deep software variability.
Our observation is that multiple layers — hardware, operating systems, third-party libraries, software versions, input data, compile-time options, and parameters — are subject to variability that exacerbates frictions but is also essential for achieving robust, generalizable results and fostering innovation. I will first review the literature, providing evidence of how the complex variability interactions across these layers affect qualitative and quantitative software properties, thereby complicating the reproduction and replication of scientific studies in various fields.
I will then present some software engineering and AI techniques that can support the strategic exploration of variability spaces. These include the use of abstractions and models (e.g., feature models), sampling strategies (e.g., uniform, random), cost-effective measurements (e.g., incremental build of software configurations), and dimensionality reduction methods (e.g., transfer learning, feature selection, software debloating).
I will finally argue that deep variability is both the problem and solution of frictionless reproducibility, calling the software science community to develop new methods and tools to manage variability and foster reproducibility in software systems.
Exposé invité Journées Nationales du GDR GPL 2024
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Phenomics assisted breeding in crop improvementIshaGoswami9
As the population is increasing and will reach about 9 billion upto 2050. Also due to climate change, it is difficult to meet the food requirement of such a large population. Facing the challenges presented by resource shortages, climate
change, and increasing global population, crop yield and quality need to be improved in a sustainable way over the coming decades. Genetic improvement by breeding is the best way to increase crop productivity. With the rapid progression of functional
genomics, an increasing number of crop genomes have been sequenced and dozens of genes influencing key agronomic traits have been identified. However, current genome sequence information has not been adequately exploited for understanding
the complex characteristics of multiple gene, owing to a lack of crop phenotypic data. Efficient, automatic, and accurate technologies and platforms that can capture phenotypic data that can
be linked to genomics information for crop improvement at all growth stages have become as important as genotyping. Thus,
high-throughput phenotyping has become the major bottleneck restricting crop breeding. Plant phenomics has been defined as the high-throughput, accurate acquisition and analysis of multi-dimensional phenotypes
during crop growing stages at the organism level, including the cell, tissue, organ, individual plant, plot, and field levels. With the rapid development of novel sensors, imaging technology,
and analysis methods, numerous infrastructure platforms have been developed for phenotyping.
ANAMOLOUS SECONDARY GROWTH IN DICOT ROOTS.pptxRASHMI M G
Abnormal or anomalous secondary growth in plants. It defines secondary growth as an increase in plant girth due to vascular cambium or cork cambium. Anomalous secondary growth does not follow the normal pattern of a single vascular cambium producing xylem internally and phloem externally.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
Travis Hills' Endeavors in Minnesota: Fostering Environmental and Economic Pr...Travis Hills MN
Travis Hills of Minnesota developed a method to convert waste into high-value dry fertilizer, significantly enriching soil quality. By providing farmers with a valuable resource derived from waste, Travis Hills helps enhance farm profitability while promoting environmental stewardship. Travis Hills' sustainable practices lead to cost savings and increased revenue for farmers by improving resource efficiency and reducing waste.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
ESR spectroscopy in liquid food and beverages.pptxPRIYANKA PATEL
With increasing population, people need to rely on packaged food stuffs. Packaging of food materials requires the preservation of food. There are various methods for the treatment of food to preserve them and irradiation treatment of food is one of them. It is the most common and the most harmless method for the food preservation as it does not alter the necessary micronutrients of food materials. Although irradiated food doesn’t cause any harm to the human health but still the quality assessment of food is required to provide consumers with necessary information about the food. ESR spectroscopy is the most sophisticated way to investigate the quality of the food and the free radicals induced during the processing of the food. ESR spin trapping technique is useful for the detection of highly unstable radicals in the food. The antioxidant capability of liquid food and beverages in mainly performed by spin trapping technique.
Salas, V. (2024) "John of St. Thomas (Poinsot) on the Science of Sacred Theol...Studia Poinsotiana
I Introduction
II Subalternation and Theology
III Theology and Dogmatic Declarations
IV The Mixed Principles of Theology
V Virtual Revelation: The Unity of Theology
VI Theology as a Natural Science
VII Theology’s Certitude
VIII Conclusion
Notes
Bibliography
All the contents are fully attributable to the author, Doctor Victor Salas. Should you wish to get this text republished, get in touch with the author or the editorial committee of the Studia Poinsotiana. Insofar as possible, we will be happy to broker your contact.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
2. ELISA
ELISA (Enzyme linked immuno-sorbent assay) is one
of the immunoassay method used to detection of
1) ANTIBODIES
2) PROTEINS
3) PEPTIDES
4) BIOMOLECULES
3. WHAT IS IMMUNOASSAY?
•The term “immunoassay” is a combined
term of “immuno”=(practically antigen-
antibody reaction) and
• “Assay”=(Determination of purity of
substance or the amount of any constituent
of a mixture.
4. WHY KNOWN AS…?
Enzyme Linked Immuno-sorbent Assay
1.Antigen/Antibody of interest is absorbed on to plastic
surface (‘Sorbent’)
2.Antigen is recognized by specific antibody (‘Immuno’)
3.This antibody recognized by second antibody
(‘immuno’) which has enzyme attached(‘enzyme
linked’)
4.Substrate react with enzyme to produce product usually
colored.
5. HISTORY OF ELISA
Radioimmunoassay was first described in a scientific
paper by Rosalyn Sussman Yalow and Solomon
Berson Published in 1960.
In 1971, Peter perlmann and Eva Engvall stockhlom
University in sweeden,and anton schuurs and Bauke
van Wccmen in the Netherlands independently
published papers that synthesized this knowledge into
methods to perform EIA/ELISA.
7. 1.ANTIBODY(ANTISERUM)
Antibody:proteins produced by the immune system
which help defend against antigens
Symbol for antibody
The variable regions are though to be the place for
recognition and binding with antigen.
8. 2.ANTIGEN
• Any molecules that induces production of antibodies
when itroduced in the body is called antigen,
OR
• Any “thing” foreign to the immune system eg.bacteria
viruses,(or their parts), pollen..etc.
9. 3.LABELLING MATERIALS
In immunoassay, it is necessary to use any marker to
know the antigen-antibody binding.for such purpose,
we label either antigen or antibody with some
materials that do not interfere with the binding.
eg.Horseradishperoxidase ezyme Substrate
:trimethylbenzidine
11. Basic Principle of ELISA
Use an enzyme to detect the binding of antigen(Ag)
antibody(Ab)
The enzyme convert a colourless substrate
(chromogen)to coloured product,indicating the
presence of Ag-Ab binding.
An elisa can be used to detect either the presence of
antigen or antibodies in a sample depending how the
test is designed
Elisa was developed in 1970 and become rapidly
accepted
12.
13. ELISA Quantitative/Qualitative
Qualitative
determines antigen or antibody is present or absent
Quantitative
o determine the quantity of antibody
o titer
o the highest dilution of the specimen usually
serum which gives positive reaction in the test
14.
15. ADVANTAGES OF ELISA
Reagents are relatively cheap and have long shelf life
ELISA is highly specific and sensitive
No radiations hazards occur during labelling or
disposal of waste.
Easy to perform and quick procedures
Equipment can be inexpensive and widely available.
ELISA can be used to variety of infections.
16. DISADVANTAGES OF ELISA
Measurement of enzyme activity can be more
complex than measurement of activity of some type of
radioisotopes.
Enzyme activity may be affected by plasma
constituent.
Kits are commercially available ,but not cheap
Very specific to a particular antigen. Won’t recognize
any other antigen.
False positive/negative possible, especially with
mutated/altered antigen
18. Common Steps…
1.Collection and processing of serum
2.Coating of wells antibodies/antigens
3.Washing
4.Incubation with the test sample
5.Washing
6.Incubation with enzyme conjugated antibodies
7.Washing again
8.Colour development by adding chromogenic substrate
9.Stopping the color development
10.Reading of results
19.
20. APPLICATIONS OF ELISA..
1.To detect Viral Contamination (HIV, Hepatitis C)
2.Hormone levels (HGH, Testosterone, ACTH)
3.Various Infections (Dengue, Human Papilloma Virus)
4.Specific disease factors (Clotting factor VII,
Lyme’s disease)
5.Drugs (Narcotics, Antipsychotics, Antidepressants)
6.Allergens in food (egg protein, milk protein) Residues in food
(Antibiotic, lupine)
7.Toxins in food (Pesticides, botulinum)
8.Others (Pregnancy Test)
9.Preclinical screening