The document discusses the avidin-biotin mediated assay, where an antibody is conjugated to biotin (vitamin B) and detected using avidin or streptavidin conjugated to enzymes. The avidin-biotin interaction is used in techniques like ELISA, immunohistochemistry, blotting, and cell surface labeling. It has advantages like high sensitivity and efficiency while requiring less primary antibody, but is more expensive.

1. Dr. J. P. Saranraj
AVIDIN – BIOTIN MEDIATED
ASSAY

2. AVIDIN – BIOTIN MEDIATED ASSAY
 The Avidin – Biotin system can be incorporated
into virtually every immunoassay, whereby an
antibody is conjugated to Biotin (Vitamin – B)
and then detected with Avidin (Glycoprotein)
(found in egg) or Streptaviridin (obtained from
the Actinobacteria Streptomyces avidinii)
conjugated to various Enzymes.
 Avidin – Biotin interaction is highly involved in
ELISA, Immunohistochemistry, Blotting
techniques, Affinity purification,
Immunoprecipitation and Cell surface labelling.

4. Advantages
 Very high sensitive.
 Increase the detection efficiency.
 Used in research more than the routine studies.
 Applied to many type of Biosensors and
Nanoparticles for increasing its efficiency.
 Detection of Protein – Drug interaction.
 Required less quantities of Primary antibody than
direct methods.
 Time saving method.
Disadvantages
 More expensive.