This research is carried out in order to improve the production of eggs in indigenous chicken by reducing the inter-sequence stopped days through use of anti-prolactin agent (Bromocriptine) and serum from laying hen. Sixty-four indigenous (deshi) chickens of 20-22 weeks of age, were randomly assigned into four groups (i, j, k and l) and each group consisting of 16 hens. Control was designated as Group I and Bromocriptine orally at a dose of 641μg/bird/day was used to treat group j, group k was treated with serum of laying kadhaknath hen serum at a dose of 1 ml intramuscularly/bird/day and group l was treated with both Kadhaknath serum and Bromocriptine at doses given to group j and k for the period of 15 March, 2019 to 16 June, 2019 and egg production, stopped days, prolactin level, hematological parameter and egg qualities were observed. A significant increase (p<0.05) in Egg production was noticed in all treated groups in comparison to the groups which were in non- treated control and group k showed the highest production. All treatment groups depicted a significant decrease (p<0.05) in stopped days and prolactin levels and lowest were observed in hens of group l. In hematological values between the chicken group, no significant differences were noticed. The present study reveals that combined treatment with Bromocriptine and serum from laying kadhaknath hen increases egg production without affecting the health of indigenous chickens.