Influence of short- and long-term administration of Melengestrol acetate on e...IJEABJ
In Egypt, research focusing on estrous synchronization in small ruminants based on Melengestrol acetate (MGA) supplementation, particularly in nulliparous ewes, is still lacking. The present work aimed to evaluate effect of long-term and short-term administration of melengestrol acetate (MGA) treatments on estrus synchronization and reproductive performance of nulliparous Barki Ewes. This study was performed in Siwa Oasis Research Station (Tegzerty Experimental Farm for animal production), belonged to Desert Research Center, Egypt. Forty five nulliparous Barki ewes with age ranging from 15.5 to 16.5 months, and 38 ± 0.23 kg average live body weight were assigned to one of three groups: (1) control (C, n = 15); (2) long-term treatment with MGA (n = 15, 0.22 mg/ewe/d for 14 days) and (3) short-term treatment with MGA (n = 15, 0.22 mg/ewe/d for 7 days). At the end of MGA treatment (14 or 7 d) all treated ewes were injected by 600 IU PMSG intramuscularly. The results showed that, ewes treated with MGA exhibited highest (P<0.05) estrus response rate (100%) in short term-MGA, followed by long term-MGA (93.33%), whereas the lowest was observed in control group (80%). Conception rates after natural mating were 85.71% and 93.33% for long term and short term MGA treated ewes, respectively. However, it recorded 100% in the control group. Fertility rate was significantly the highest (P<0.05) in short term-MGA (93.33%) than other groups (73.33%). There were no significant differences (p>0.05) in terms of lambing rate and prolificacy among the control and treated ewes. In the meantime, mean values of serum estradiol 17-β were lower (P≤0.01) in long-term treated ewes compared to those of short-term treated ones (27.20±1.78, 32.67±1.27pg/ml), respectively, while the lowest (P<0.05) level was recorded in the control ewes (13.01±1.31 pg/ml). Furthermore, overall mean values of serum progesterone in the control group (1.32 ± 0.09 ng/ml) was higher (P<0.05) than those of long- and short-term MGA treated groups (1.01 ± 0.13, 0.92 ± 0.11, ng/ml), respectively. It is concluded that reproductive efficiency of nulliparous Barki ewes could be improved by short-term supplementation with MGA.
- This experiment was conducted to study the
chronological relationships between estrus onset, time of LH
surge and ovulation time in does synchronized with chronogest
or CIDR. Forty six desert goats were divided into 2 groups (A,
and B). Induction of oestrous was carried out using
intravaginal sponges impregnated with 40 mg fluorgestone and
CIDR for group A and B respectively. At time of sponge or
CIDR removal, the animals were injected with 300IU eCG.
There was no significant difference in the estrus onset, estrous
duration, ovulation time and time of LH surge in does
synchronized with either chronogest or CIDR. Ovulation was
detected 54 hours post sponges or CIDR removal. LH surge
occurred 28 hours post removal of sponge or CIDR. No
significant difference was detected with regard to the time
taken from oestrus onset to ovulation time (25.4 and 23.5 hours
respectively). Also, there was no significant difference in the
time taken from LH surge to ovulation time in does
synchronized with chronogest and CIDR (25.6 and 25.3 hours)
respectively. In conclusion, findings observed pertaining to
estrous onset, time of ovulation and the time of onset of the LH
peak could be of value in timing AI or mating for maximum
conception in goat breeding programs when CIDR or sponges
were used with 300IU eCG.
This study aimed to evaluate the efficacy of ivermectin, fenbendazole and albendazole against gastrointestinal nematodes in naturally infected goats of government goat development farm, Sylhet, Bangladesh. The study included 50 black Bengal breed of which 30 were naturally infected and randomly selected 20 on the basis of their weight and egg count. Twenty black Bengal goats of 13-15 month old irrespective of sex infested with gastrointestinal nematodes were selected for this experiment and randomly divided into four equal groups (group A, B, C and D) where each group consisted of 5 goats and goats of group D were kept as control group. One injectable ivermectin (200μgkg-1 body weight, S/C) preparations (Techno Drugs Limited, Bangladesh) and two solid fenbendazole, albendazole (7.5 mgkg-1 body weight, orally) preparations (Techno Drugs Limited and Square Pharmaceuticals Ltd, Bangladesh) were used for positive control of gastrointestinal nematodes as group A, B and C. Goats of group D was kept as control without giving any treatment. Before trials (day 0), total egg count, blood samples and initial body weight were recorded. During the study period the faecal and blood samples were collected directly from rectum and examined on 7th, 14th, 21st and 28th day using McMaster fecal egg counting method. Body weight were recorded on day 28 following the treatments. The results of the comparative efficacies of different anthelmintic of ivermectin was 100%, followed by fenbendazole 95.33% and albendazole 90.11%. McMaster fecal egg counting method disclose the percentage of Haemonchus spp. (15.38%), with Trichostrongylus spp, Strongyloides spp., and Cooperia spp. also present. The body weight of the treated animals were slightly increased which were significant (p<0.05). After treatment with ivermectin, fenbendazole and albendazole, Total Erythrocyte Count (TEC), Hemoglobin (Hb) content and Packed Cell Volume (PCV) were increased significantly (p>0.05 and p<0.01) in goats but Erythrocyte Sedimentation Rate (ESR) and Total Leukocyte Count (TLC) were decreased significantly (p>0.05) in all treated goat and body weight was increased significantly (p<0.01) on day 28. The farm management practices along with results of the present study revealed the efficacy of multiple anthelmintics against gastrointestinal nematodes in goats. Additional detailed studies are required to clarify the current status of the efficacy of the anthelmintics widely used in different agro ecologies, animal species, and livestock management systems in Bangladesh
Fertility Response Following Induction of Lactation in Infertile Dairy Cowsijtsrd
The fertility response following induction protocol in infertile dry cows was assessed in terms of the induction of oestrus in anoestrus cows and conception in repeat breeders cows in the study. There were four anoestrous and two repeat breeder cows in G-1 out of them two anoestrus became cyclic, one repeater animal conceived after treatment protocol. Where as in G-2, three anoestrus and three repeat breeder animals given induction protocol where two anoestrous cows became cyclic and two repeaters conceived. The analysis of data revealed higher fertility response in G-2 as compared to G-1 (66.67 Vs. 50 %, respectively) with the higher conception rate (50 Vs. 33.33 %, respectively). It indicates better fertility response in G-2 as compared to G-1. K. Kumar | S. N. Shukla | S. Bhandekar | S. K. Singh | P. Inwati"Fertility Response Following Induction of Lactation in Infertile Dairy Cows" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-2 , February 2018, URL: http://www.ijtsrd.com/papers/ijtsrd9615.pdf http://www.ijtsrd.com/other-scientific-research-area/other/9615/fertility-response-following-induction-of-lactation-in-infertile-dairy-cows/k-kumar
The objective of this study was to evaluate the effect of the systematic use of a single amount of the prostaglandin F2α after one month of calving on the renewal of sexual activity of beef at Chad. (Sixty five (65) cows of local breeds from N'Djamena urban area were selected and divided Into two groups: Forty-three (43) cows were treated with Prostaglandin F2α, one month after calving and 22 cows as witnesses. More than half (60.46%) of the cows answered the treatment and 39.54% did not react. The answer varied with the parity (68%) for the first half of the cows against 32% for the multiparous but the body weight at calving did not-have an effect. Only, the acceptance of overlapping was Retained for the detection of return in heat, which took place on an average of 2.36 ± 0.14 days after treatment. The treatment made it feasible to-have-one year interval between calving (first heat of 32.36 ± 0.14 days.) Heat thus obtained, can be used as reference to the stockbreeders to program the reproduction of their herd in order to have one year interval calving-calving closed. This test sample gave better Indication in first half of the cows while waiting for confirming it in the station.
Animal breeding is a process that is being used from many years ago. In the earlier days, the breeders use to increase the qualities in the animals by using breeding and other methods. But by the invention of the molecular techniques, it is become easier to manipulate any animal and to enhance the traits or different qualities in the animals. In this regard, different methods have used these methods are discussed in this article. The animal transgenesis is used for different purposes such as in research purposes, like organ transplant source for humans, as protein extraction sources, as drug extraction process, in hormones production, and in many other purposes.
Influence of short- and long-term administration of Melengestrol acetate on e...IJEABJ
In Egypt, research focusing on estrous synchronization in small ruminants based on Melengestrol acetate (MGA) supplementation, particularly in nulliparous ewes, is still lacking. The present work aimed to evaluate effect of long-term and short-term administration of melengestrol acetate (MGA) treatments on estrus synchronization and reproductive performance of nulliparous Barki Ewes. This study was performed in Siwa Oasis Research Station (Tegzerty Experimental Farm for animal production), belonged to Desert Research Center, Egypt. Forty five nulliparous Barki ewes with age ranging from 15.5 to 16.5 months, and 38 ± 0.23 kg average live body weight were assigned to one of three groups: (1) control (C, n = 15); (2) long-term treatment with MGA (n = 15, 0.22 mg/ewe/d for 14 days) and (3) short-term treatment with MGA (n = 15, 0.22 mg/ewe/d for 7 days). At the end of MGA treatment (14 or 7 d) all treated ewes were injected by 600 IU PMSG intramuscularly. The results showed that, ewes treated with MGA exhibited highest (P<0.05) estrus response rate (100%) in short term-MGA, followed by long term-MGA (93.33%), whereas the lowest was observed in control group (80%). Conception rates after natural mating were 85.71% and 93.33% for long term and short term MGA treated ewes, respectively. However, it recorded 100% in the control group. Fertility rate was significantly the highest (P<0.05) in short term-MGA (93.33%) than other groups (73.33%). There were no significant differences (p>0.05) in terms of lambing rate and prolificacy among the control and treated ewes. In the meantime, mean values of serum estradiol 17-β were lower (P≤0.01) in long-term treated ewes compared to those of short-term treated ones (27.20±1.78, 32.67±1.27pg/ml), respectively, while the lowest (P<0.05) level was recorded in the control ewes (13.01±1.31 pg/ml). Furthermore, overall mean values of serum progesterone in the control group (1.32 ± 0.09 ng/ml) was higher (P<0.05) than those of long- and short-term MGA treated groups (1.01 ± 0.13, 0.92 ± 0.11, ng/ml), respectively. It is concluded that reproductive efficiency of nulliparous Barki ewes could be improved by short-term supplementation with MGA.
- This experiment was conducted to study the
chronological relationships between estrus onset, time of LH
surge and ovulation time in does synchronized with chronogest
or CIDR. Forty six desert goats were divided into 2 groups (A,
and B). Induction of oestrous was carried out using
intravaginal sponges impregnated with 40 mg fluorgestone and
CIDR for group A and B respectively. At time of sponge or
CIDR removal, the animals were injected with 300IU eCG.
There was no significant difference in the estrus onset, estrous
duration, ovulation time and time of LH surge in does
synchronized with either chronogest or CIDR. Ovulation was
detected 54 hours post sponges or CIDR removal. LH surge
occurred 28 hours post removal of sponge or CIDR. No
significant difference was detected with regard to the time
taken from oestrus onset to ovulation time (25.4 and 23.5 hours
respectively). Also, there was no significant difference in the
time taken from LH surge to ovulation time in does
synchronized with chronogest and CIDR (25.6 and 25.3 hours)
respectively. In conclusion, findings observed pertaining to
estrous onset, time of ovulation and the time of onset of the LH
peak could be of value in timing AI or mating for maximum
conception in goat breeding programs when CIDR or sponges
were used with 300IU eCG.
This study aimed to evaluate the efficacy of ivermectin, fenbendazole and albendazole against gastrointestinal nematodes in naturally infected goats of government goat development farm, Sylhet, Bangladesh. The study included 50 black Bengal breed of which 30 were naturally infected and randomly selected 20 on the basis of their weight and egg count. Twenty black Bengal goats of 13-15 month old irrespective of sex infested with gastrointestinal nematodes were selected for this experiment and randomly divided into four equal groups (group A, B, C and D) where each group consisted of 5 goats and goats of group D were kept as control group. One injectable ivermectin (200μgkg-1 body weight, S/C) preparations (Techno Drugs Limited, Bangladesh) and two solid fenbendazole, albendazole (7.5 mgkg-1 body weight, orally) preparations (Techno Drugs Limited and Square Pharmaceuticals Ltd, Bangladesh) were used for positive control of gastrointestinal nematodes as group A, B and C. Goats of group D was kept as control without giving any treatment. Before trials (day 0), total egg count, blood samples and initial body weight were recorded. During the study period the faecal and blood samples were collected directly from rectum and examined on 7th, 14th, 21st and 28th day using McMaster fecal egg counting method. Body weight were recorded on day 28 following the treatments. The results of the comparative efficacies of different anthelmintic of ivermectin was 100%, followed by fenbendazole 95.33% and albendazole 90.11%. McMaster fecal egg counting method disclose the percentage of Haemonchus spp. (15.38%), with Trichostrongylus spp, Strongyloides spp., and Cooperia spp. also present. The body weight of the treated animals were slightly increased which were significant (p<0.05). After treatment with ivermectin, fenbendazole and albendazole, Total Erythrocyte Count (TEC), Hemoglobin (Hb) content and Packed Cell Volume (PCV) were increased significantly (p>0.05 and p<0.01) in goats but Erythrocyte Sedimentation Rate (ESR) and Total Leukocyte Count (TLC) were decreased significantly (p>0.05) in all treated goat and body weight was increased significantly (p<0.01) on day 28. The farm management practices along with results of the present study revealed the efficacy of multiple anthelmintics against gastrointestinal nematodes in goats. Additional detailed studies are required to clarify the current status of the efficacy of the anthelmintics widely used in different agro ecologies, animal species, and livestock management systems in Bangladesh
Fertility Response Following Induction of Lactation in Infertile Dairy Cowsijtsrd
The fertility response following induction protocol in infertile dry cows was assessed in terms of the induction of oestrus in anoestrus cows and conception in repeat breeders cows in the study. There were four anoestrous and two repeat breeder cows in G-1 out of them two anoestrus became cyclic, one repeater animal conceived after treatment protocol. Where as in G-2, three anoestrus and three repeat breeder animals given induction protocol where two anoestrous cows became cyclic and two repeaters conceived. The analysis of data revealed higher fertility response in G-2 as compared to G-1 (66.67 Vs. 50 %, respectively) with the higher conception rate (50 Vs. 33.33 %, respectively). It indicates better fertility response in G-2 as compared to G-1. K. Kumar | S. N. Shukla | S. Bhandekar | S. K. Singh | P. Inwati"Fertility Response Following Induction of Lactation in Infertile Dairy Cows" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-2 , February 2018, URL: http://www.ijtsrd.com/papers/ijtsrd9615.pdf http://www.ijtsrd.com/other-scientific-research-area/other/9615/fertility-response-following-induction-of-lactation-in-infertile-dairy-cows/k-kumar
The objective of this study was to evaluate the effect of the systematic use of a single amount of the prostaglandin F2α after one month of calving on the renewal of sexual activity of beef at Chad. (Sixty five (65) cows of local breeds from N'Djamena urban area were selected and divided Into two groups: Forty-three (43) cows were treated with Prostaglandin F2α, one month after calving and 22 cows as witnesses. More than half (60.46%) of the cows answered the treatment and 39.54% did not react. The answer varied with the parity (68%) for the first half of the cows against 32% for the multiparous but the body weight at calving did not-have an effect. Only, the acceptance of overlapping was Retained for the detection of return in heat, which took place on an average of 2.36 ± 0.14 days after treatment. The treatment made it feasible to-have-one year interval between calving (first heat of 32.36 ± 0.14 days.) Heat thus obtained, can be used as reference to the stockbreeders to program the reproduction of their herd in order to have one year interval calving-calving closed. This test sample gave better Indication in first half of the cows while waiting for confirming it in the station.
Animal breeding is a process that is being used from many years ago. In the earlier days, the breeders use to increase the qualities in the animals by using breeding and other methods. But by the invention of the molecular techniques, it is become easier to manipulate any animal and to enhance the traits or different qualities in the animals. In this regard, different methods have used these methods are discussed in this article. The animal transgenesis is used for different purposes such as in research purposes, like organ transplant source for humans, as protein extraction sources, as drug extraction process, in hormones production, and in many other purposes.
dkNET Webinar: Population-Based Approaches to Investigate Endocrine Communica...dkNET
Abstract
Mechanisms of inter-organ signaling have been established as hallmarks of nearly every pathophysiologic condition, where many exist as related and complex diseases. While significant work has been focused on understanding how individual cell types contribute and respond to specific perturbations related to common, complex disease, an equally-important but relatively less-explored question involves how relationships between organs are altered in the context of an integrated living organism. Current technical advances, such as proteomic analysis of plasma or conditioned media, have allowed for a more unbiased visualization and discovery of additional inter-tissue signaling molecules. However, one important feature which is lacking from these approaches is the ability to gain insight as to the function, mechanisms of action and target tissue(s) of relevant molecules. To begin to address these constraints, we initially developed a correlation-based bioinformatics framework which uses multi-tissue gene expression and/or proteomic data, as well as publicly available resources to statistically rank and functionally annotate endocrine proteins involved in tissue cross-talk. Using this approach, we identified many known and experimentally validated several novel inter-tissue circuits. This was this first study to directly link an endocrine-focused bioinformatics pipeline from population data directly to experimentally-validated mechanisms of inter-tissue communication. While these validations provide strong support for exploiting natural variation to discover new modes of communication, these serve as simple proof-of-principle studies and, thus, have promising potential for expansion. Some of these will be discussed during the presentation.
Presenter: Marcus Seldin, Ph.D. Assistant Professor, Biological Chemistry, University of California Irvine
Upcoming webinars schedule: https://dknet.org/about/webinar
Comparative Effects of Different Feeds on Production and Reproduction of Cros...INFOGAIN PUBLICATION
The present study was conducted from January to June 2014, in Gangachara Upazila of Rangpur District, Bangladesh. A sample of 60 cross-bred dairy cows: Local × Holstein Friesian (L×HF) = 30 and Local × Sahiwal (L×Sh) = 30 were selected for in depth study. The average age at first heat, services per conception, age at first calving, post-partum heat period, the average milk production, the average lactation period were observed after feeding three types of feed: type-I (Sweet jamboo grass), type-II (Jomjom Dairy feed) and type-III (Advance Chemical Industry) feeding system. Local × Holstein Friesian (L×HF) cross and Local × Sahiwal (L×Sh) cross cows showed significantly (p<0.05) better results by type-III feeding system compare with the type-I and type-II feeding system.
Effects of Ovaprim Hormone on Induced Breeding of Clarias gariepinusBRNSS Publication Hub
This study was conducted to determine the artificial breeding with application of optimum dosage of stimulatory Ovaprim hormones. Female treated with T1 (0.3), T2 (0.4), T3 (0.5), and T4(control) ml/kg/body weight. The result showed that stimulated with T3 (0.5) obtained better eggs quantity (25006) followed by T2 (0.4) (17,200), while the lowest quantity (8233) was in T1 (0.3), but T4(control) was failed. The spawning hours, fertilization and hatchability, was significantly affected by three doses (P ˂ 0.05). The hatchability hours was not significantly affected by hormone doses (P ˃ 0.05). The survival rate was significantly affected by hormone doses (P ˂ 0.05). The highest survival rates (53.67%) observed in T3 (0.5) followed by T2 (0.4) (42%) while the lowest (32.67%) in T1 (0.3).
Screening Fenugreek genotypes for high callus induction and growth | IJAARInnspub Net
Fenugreek (Trigonella foenume-graecum) is a medicinal herb that has anti-diabetic properties. 4-hydroxyisoleucine, an important metabolite of this plant, is a good candidate for improving hepatic insulin resistance in type 2 diabetic patients. In vitro production of metabolites mainly relies on the ability of the tissue culture system to produce more and viable cells with maximum production rate. Callus induction is the first step of establishing plant cell based bioreactors which needs potential genotypes with higher and faster callus production. To find such genotypes, screening them is indispensable. In this research, we screened 21 Iranian landraces of fenugreek for callus induction and growth on MS medium supplemented with some plant growth regulators. The results showed different capacity of the screened genotypes in callus induction (P<0.05). Also, the type of explant had a significant effect on callus induction (P<0.05). Calli were able to produce 4-Hydroxyisulosine 67% of the amount in vivo samples.
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
Methimazole affected spermatogenesis and enhanced proliferation of testicular...Jing Zang
The present work studied the effect of the antithyroid drug, methemazole (MMI) in testis of rat. Moreover, the effect of MMI on testicular macrophages was studied. The MMI-treated rats received diet food and 0.1% methimazole drinking water for 30 days. The results showed that MMI caused reduction in body weight of the animals. Histological examination of the testis revealed significant decrease in diameter of seminiferous tubules and inhibition of spermatogenesis. A significant increase in the number of macrophages was recorded in the testicular interstitium. The highest number of macrophages was found in close proximity to Leydig cells followed by peritubular location. The lowest number was observed in perivascular location. Macrophages are necessary to remove cellular remnant like apoptotic material or cell debris in inflammable tissue. So the increase in macrophage number recorded in this work suggests that either apoptotic or inflammable processes rise in rat testicular interstitium after exposure to methimazole.
dkNET Webinar: A New Approach to the Study of Energy Balance and Obesity usin...dkNET
Abstract
We report a web-based tool for analysis of experiments using indirect calorimetry to measure physiological energy balance. CalR simplifies the process to import raw data files, generate plots, and determine the most appropriate statistical tests for interpretation. Analysis using the generalized linear model (which includes ANOVA and ANCOVA) allows for flexibility in interpreting diverse experimental designs, including those of obesity and thermogenesis. Users also may produce standardized output files for an experiment that can be shared and subsequently re-evaluated using CalR. This framework will provide the transparency necessary to enhance consistency, rigor, and reproducibility. The CalR analysis software will greatly increase the speed and efficiency with which metabolic experiments can be organized, analyzed per accepted norms, and reproduced and has become a standard tool for the field. CalR is accessible at https://CalRapp.org/
The top 4 key questions that our tool can answer:
1. Can I reproducibly and transparently analyze indirect calorimetry experiments in under 10 minutes?
2. How hard is it to use Analysis of Covariance (ANCOVA) to determine whether my groups of animals are significantly different?
3. Is there an automated, reproducible way to exclude “noisy” outlier data from our indirect calorimetry experiments?
4. What are the key factors in determining metabolic rate of mice?
Presenter: Alexander Banks, PhD, principal investigator and assistant professor at Harvard Medical School and the Beth Israel Deaconess Medical Center.
dkNET Webinar Information: https://dknet.org/about/webinar
The relationship between progesterone and biochemical constituents of amnioti...Ali Olfati
Ali Olfati1, Gholamali Moghaddam1, Nasroallah Moradi Kor2*, Mitra Bakhtiari3
1Department of Animal Science, Faculty of Agriculture, University of Tabriz, Iran
2Department of Reproduction Physiologies, Iranian Society of Physiology and Pharmacology, Tehran, Iran
3Department of Anatomical Sciences, Faculty of Medicine, University of Medical Sciences, Kermanshah, Iran
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
Correlation and Path Analysis of Groundnut (Arachis hypogaea L.) Genotypes in...Premier Publishers
Sixteen groundnut genotypes (including local check) were evaluated for quantitative parameters. The crop was sown during 2015 Ethiopian wet season in four locations. The experiment was laid out in Randomized Complete Block Design (RCBD) with three replications. Twelve agromorphological characters were evaluated and the covariance, coheritability, phenotypic, genotypic and environmental correlations and also the direct and indirect effects of the component variables on grain yield/ha were estimated. The results indicated that genotypic correlations were higher than the phenotypic and environmental ones. The grain yield/ha presented positive and significant genetic correlation with PWP, SWP and 100SW. Path analysis based on genotypic and phenotypic correlations showed that direct effects were generally lower than indirect effects on the grain yield showing that no best character contributes to GY/ha. Instead, characters like NMP, PWP, SWP, 100SW, NSPOD and AGBP should be recommended for groundnut breeding for increasing GY/ha.
DOI:10.21276/ijlssr.2016.2.4.17
ABSTRACT- Present study was undertaken to evaluate the reversible anti-fertility effect of Ocimum (Tulsi) on male mice. Aqueous leaf extract of Ocimum sanctum was orally administered (0.1ml) for 10 (P < s0a.0n5ct)u, m20 L(Pin <n>< e0d. 0s0ig1n),i f4ic0a (nPt d<e><o><p0o.s1u),r><t0m.0e1n)t,><e0o.u0s1><a0c.t0><<00..0001)1,)><re0a.t0m1e),n><th0e.><ti0l.i0ty0><a0><no0r.0m0a1l)i><sp0e.0rm01a)t><s0e.d0><ec0r.e0a1s)e,><rm0.><c0a.u0s0e1d)><w0e.i0g1h)t><v0i.z0.0,><c0a.n0t0ly1>< t0o. 0t1h)e, acnony trtorel agtrmoeunpt. Ttoh ec hreeccokv ethrye grerovuerps iobfi laitnyi.m Aall,l wthhei cahn aimlsaol sr eacfetievre dth 5e0 r decaoyvs etrryea ptmereinotd, wshaosw meadi nntaoirnmeadl foferr 9ti0li tdya yrast ew. itThhouust cOocnimcluudme sthaantc Otucmim audmv esrasneclytu maf fceacnts b efe urtsielidt ya si na pmotiecne t aanndti -fsehrotwilietyd aagnetni-tf werhtiilcithy ise frfeevcetr saimbloe.n g them. From this we can Key-words- Ocimum sanctum, Anti-fertility, Sperm count, Motility, Sperm abnormality
This research is carried out in order to improve the production of eggs in indigenous chicken by reducing the
inter-sequence stopped days through use of anti-prolactin agent (Bromocriptine) and serum from laying hen.
Sixty-four indigenous (deshi) chickens of 20-22 weeks of age, were randomly assigned into four groups (i, j, k
and l) and each group consisting of 16 hens. Control was designated as Group I and Bromocriptine orally at a
dose of 641μg/bird/day was used to treat group j, group k was treated with serum of laying kadhaknath hen
serum at a dose of 1 ml intramuscularly/bird/day and group l was treated with both Kadhaknath serum and
Bromocriptine at doses given to group j and k for the period of 15 March, 2019 to 16 June, 2019 and egg
production, stopped days, prolactin level, hematological parameter and egg qualities were observed. A
significant increase (p<0.05) in Egg production was noticed in all treated groups in comparison to the groups
which were in non- treated control and group k showed the highest production. All treatment groups depicted a
significant decrease (p<0.05) in stopped days and prolactin levels and lowest were observed in hens of group l.
In hematological values between the chicken group, no significant differences were noticed. The present study
reveals that combined treatment with Bromocriptine and serum from laying kadhaknath hen increases egg
production without affecting the health of indigenous chickens.
Prevalence of Toxoplasma gondii infection in domestic animals in District Ban...Open Access Research Paper
Toxoplasma gondii is an intracellular zoonotic protozoan parasite, infect both humans and animals population worldwide. It can also cause abortion and inborn disease in humans and livestock population. In the present study total of 313 domestic animals were screened for Toxoplasma gondii infection. Of which 45 cows, 55 buffalos, 68 goats, 60 sheep and 85 shaver chicken were tested. Among these 40 (88.88%) cows were negative and 05 (11.12%) were positive. Similarly 55 (92.72%) buffalos were negative and 04 (07.28%) were positive. In goats 68 (98.52%) were negative and 01 (01.48%) was recorded positive. In sheep and shaver chicken the infection were not recorded.
Prevalence of Intestinal Helminths and Protozoa Parasites of Ruminants in Min...iosrjce
IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS) is a double blind peer reviewed International Journal edited by the International Organization of Scientific Research (IOSR). The journal provides a common forum where all aspects of Agricultural and Veterinary Sciences are presented. The journal invites original papers, review articles, technical reports and short communications containing new insight into any aspect Agricultural and Veterinary Sciences that are not published or not being considered for publication elsewhere.
dkNET Webinar: Population-Based Approaches to Investigate Endocrine Communica...dkNET
Abstract
Mechanisms of inter-organ signaling have been established as hallmarks of nearly every pathophysiologic condition, where many exist as related and complex diseases. While significant work has been focused on understanding how individual cell types contribute and respond to specific perturbations related to common, complex disease, an equally-important but relatively less-explored question involves how relationships between organs are altered in the context of an integrated living organism. Current technical advances, such as proteomic analysis of plasma or conditioned media, have allowed for a more unbiased visualization and discovery of additional inter-tissue signaling molecules. However, one important feature which is lacking from these approaches is the ability to gain insight as to the function, mechanisms of action and target tissue(s) of relevant molecules. To begin to address these constraints, we initially developed a correlation-based bioinformatics framework which uses multi-tissue gene expression and/or proteomic data, as well as publicly available resources to statistically rank and functionally annotate endocrine proteins involved in tissue cross-talk. Using this approach, we identified many known and experimentally validated several novel inter-tissue circuits. This was this first study to directly link an endocrine-focused bioinformatics pipeline from population data directly to experimentally-validated mechanisms of inter-tissue communication. While these validations provide strong support for exploiting natural variation to discover new modes of communication, these serve as simple proof-of-principle studies and, thus, have promising potential for expansion. Some of these will be discussed during the presentation.
Presenter: Marcus Seldin, Ph.D. Assistant Professor, Biological Chemistry, University of California Irvine
Upcoming webinars schedule: https://dknet.org/about/webinar
Comparative Effects of Different Feeds on Production and Reproduction of Cros...INFOGAIN PUBLICATION
The present study was conducted from January to June 2014, in Gangachara Upazila of Rangpur District, Bangladesh. A sample of 60 cross-bred dairy cows: Local × Holstein Friesian (L×HF) = 30 and Local × Sahiwal (L×Sh) = 30 were selected for in depth study. The average age at first heat, services per conception, age at first calving, post-partum heat period, the average milk production, the average lactation period were observed after feeding three types of feed: type-I (Sweet jamboo grass), type-II (Jomjom Dairy feed) and type-III (Advance Chemical Industry) feeding system. Local × Holstein Friesian (L×HF) cross and Local × Sahiwal (L×Sh) cross cows showed significantly (p<0.05) better results by type-III feeding system compare with the type-I and type-II feeding system.
Effects of Ovaprim Hormone on Induced Breeding of Clarias gariepinusBRNSS Publication Hub
This study was conducted to determine the artificial breeding with application of optimum dosage of stimulatory Ovaprim hormones. Female treated with T1 (0.3), T2 (0.4), T3 (0.5), and T4(control) ml/kg/body weight. The result showed that stimulated with T3 (0.5) obtained better eggs quantity (25006) followed by T2 (0.4) (17,200), while the lowest quantity (8233) was in T1 (0.3), but T4(control) was failed. The spawning hours, fertilization and hatchability, was significantly affected by three doses (P ˂ 0.05). The hatchability hours was not significantly affected by hormone doses (P ˃ 0.05). The survival rate was significantly affected by hormone doses (P ˂ 0.05). The highest survival rates (53.67%) observed in T3 (0.5) followed by T2 (0.4) (42%) while the lowest (32.67%) in T1 (0.3).
Screening Fenugreek genotypes for high callus induction and growth | IJAARInnspub Net
Fenugreek (Trigonella foenume-graecum) is a medicinal herb that has anti-diabetic properties. 4-hydroxyisoleucine, an important metabolite of this plant, is a good candidate for improving hepatic insulin resistance in type 2 diabetic patients. In vitro production of metabolites mainly relies on the ability of the tissue culture system to produce more and viable cells with maximum production rate. Callus induction is the first step of establishing plant cell based bioreactors which needs potential genotypes with higher and faster callus production. To find such genotypes, screening them is indispensable. In this research, we screened 21 Iranian landraces of fenugreek for callus induction and growth on MS medium supplemented with some plant growth regulators. The results showed different capacity of the screened genotypes in callus induction (P<0.05). Also, the type of explant had a significant effect on callus induction (P<0.05). Calli were able to produce 4-Hydroxyisulosine 67% of the amount in vivo samples.
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
Methimazole affected spermatogenesis and enhanced proliferation of testicular...Jing Zang
The present work studied the effect of the antithyroid drug, methemazole (MMI) in testis of rat. Moreover, the effect of MMI on testicular macrophages was studied. The MMI-treated rats received diet food and 0.1% methimazole drinking water for 30 days. The results showed that MMI caused reduction in body weight of the animals. Histological examination of the testis revealed significant decrease in diameter of seminiferous tubules and inhibition of spermatogenesis. A significant increase in the number of macrophages was recorded in the testicular interstitium. The highest number of macrophages was found in close proximity to Leydig cells followed by peritubular location. The lowest number was observed in perivascular location. Macrophages are necessary to remove cellular remnant like apoptotic material or cell debris in inflammable tissue. So the increase in macrophage number recorded in this work suggests that either apoptotic or inflammable processes rise in rat testicular interstitium after exposure to methimazole.
dkNET Webinar: A New Approach to the Study of Energy Balance and Obesity usin...dkNET
Abstract
We report a web-based tool for analysis of experiments using indirect calorimetry to measure physiological energy balance. CalR simplifies the process to import raw data files, generate plots, and determine the most appropriate statistical tests for interpretation. Analysis using the generalized linear model (which includes ANOVA and ANCOVA) allows for flexibility in interpreting diverse experimental designs, including those of obesity and thermogenesis. Users also may produce standardized output files for an experiment that can be shared and subsequently re-evaluated using CalR. This framework will provide the transparency necessary to enhance consistency, rigor, and reproducibility. The CalR analysis software will greatly increase the speed and efficiency with which metabolic experiments can be organized, analyzed per accepted norms, and reproduced and has become a standard tool for the field. CalR is accessible at https://CalRapp.org/
The top 4 key questions that our tool can answer:
1. Can I reproducibly and transparently analyze indirect calorimetry experiments in under 10 minutes?
2. How hard is it to use Analysis of Covariance (ANCOVA) to determine whether my groups of animals are significantly different?
3. Is there an automated, reproducible way to exclude “noisy” outlier data from our indirect calorimetry experiments?
4. What are the key factors in determining metabolic rate of mice?
Presenter: Alexander Banks, PhD, principal investigator and assistant professor at Harvard Medical School and the Beth Israel Deaconess Medical Center.
dkNET Webinar Information: https://dknet.org/about/webinar
The relationship between progesterone and biochemical constituents of amnioti...Ali Olfati
Ali Olfati1, Gholamali Moghaddam1, Nasroallah Moradi Kor2*, Mitra Bakhtiari3
1Department of Animal Science, Faculty of Agriculture, University of Tabriz, Iran
2Department of Reproduction Physiologies, Iranian Society of Physiology and Pharmacology, Tehran, Iran
3Department of Anatomical Sciences, Faculty of Medicine, University of Medical Sciences, Kermanshah, Iran
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
Correlation and Path Analysis of Groundnut (Arachis hypogaea L.) Genotypes in...Premier Publishers
Sixteen groundnut genotypes (including local check) were evaluated for quantitative parameters. The crop was sown during 2015 Ethiopian wet season in four locations. The experiment was laid out in Randomized Complete Block Design (RCBD) with three replications. Twelve agromorphological characters were evaluated and the covariance, coheritability, phenotypic, genotypic and environmental correlations and also the direct and indirect effects of the component variables on grain yield/ha were estimated. The results indicated that genotypic correlations were higher than the phenotypic and environmental ones. The grain yield/ha presented positive and significant genetic correlation with PWP, SWP and 100SW. Path analysis based on genotypic and phenotypic correlations showed that direct effects were generally lower than indirect effects on the grain yield showing that no best character contributes to GY/ha. Instead, characters like NMP, PWP, SWP, 100SW, NSPOD and AGBP should be recommended for groundnut breeding for increasing GY/ha.
DOI:10.21276/ijlssr.2016.2.4.17
ABSTRACT- Present study was undertaken to evaluate the reversible anti-fertility effect of Ocimum (Tulsi) on male mice. Aqueous leaf extract of Ocimum sanctum was orally administered (0.1ml) for 10 (P < s0a.0n5ct)u, m20 L(Pin <n>< e0d. 0s0ig1n),i f4ic0a (nPt d<e><o><p0o.s1u),r><t0m.0e1n)t,><e0o.u0s1><a0c.t0><<00..0001)1,)><re0a.t0m1e),n><th0e.><ti0l.i0ty0><a0><no0r.0m0a1l)i><sp0e.0rm01a)t><s0e.d0><ec0r.e0a1s)e,><rm0.><c0a.u0s0e1d)><w0e.i0g1h)t><v0i.z0.0,><c0a.n0t0ly1>< t0o. 0t1h)e, acnony trtorel agtrmoeunpt. Ttoh ec hreeccokv ethrye grerovuerps iobfi laitnyi.m Aall,l wthhei cahn aimlsaol sr eacfetievre dth 5e0 r decaoyvs etrryea ptmereinotd, wshaosw meadi nntaoirnmeadl foferr 9ti0li tdya yrast ew. itThhouust cOocnimcluudme sthaantc Otucmim audmv esrasneclytu maf fceacnts b efe urtsielidt ya si na pmotiecne t aanndti -fsehrotwilietyd aagnetni-tf werhtiilcithy ise frfeevcetr saimbloe.n g them. From this we can Key-words- Ocimum sanctum, Anti-fertility, Sperm count, Motility, Sperm abnormality
This research is carried out in order to improve the production of eggs in indigenous chicken by reducing the
inter-sequence stopped days through use of anti-prolactin agent (Bromocriptine) and serum from laying hen.
Sixty-four indigenous (deshi) chickens of 20-22 weeks of age, were randomly assigned into four groups (i, j, k
and l) and each group consisting of 16 hens. Control was designated as Group I and Bromocriptine orally at a
dose of 641μg/bird/day was used to treat group j, group k was treated with serum of laying kadhaknath hen
serum at a dose of 1 ml intramuscularly/bird/day and group l was treated with both Kadhaknath serum and
Bromocriptine at doses given to group j and k for the period of 15 March, 2019 to 16 June, 2019 and egg
production, stopped days, prolactin level, hematological parameter and egg qualities were observed. A
significant increase (p<0.05) in Egg production was noticed in all treated groups in comparison to the groups
which were in non- treated control and group k showed the highest production. All treatment groups depicted a
significant decrease (p<0.05) in stopped days and prolactin levels and lowest were observed in hens of group l.
In hematological values between the chicken group, no significant differences were noticed. The present study
reveals that combined treatment with Bromocriptine and serum from laying kadhaknath hen increases egg
production without affecting the health of indigenous chickens.
Prevalence of Toxoplasma gondii infection in domestic animals in District Ban...Open Access Research Paper
Toxoplasma gondii is an intracellular zoonotic protozoan parasite, infect both humans and animals population worldwide. It can also cause abortion and inborn disease in humans and livestock population. In the present study total of 313 domestic animals were screened for Toxoplasma gondii infection. Of which 45 cows, 55 buffalos, 68 goats, 60 sheep and 85 shaver chicken were tested. Among these 40 (88.88%) cows were negative and 05 (11.12%) were positive. Similarly 55 (92.72%) buffalos were negative and 04 (07.28%) were positive. In goats 68 (98.52%) were negative and 01 (01.48%) was recorded positive. In sheep and shaver chicken the infection were not recorded.
Prevalence of Intestinal Helminths and Protozoa Parasites of Ruminants in Min...iosrjce
IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS) is a double blind peer reviewed International Journal edited by the International Organization of Scientific Research (IOSR). The journal provides a common forum where all aspects of Agricultural and Veterinary Sciences are presented. The journal invites original papers, review articles, technical reports and short communications containing new insight into any aspect Agricultural and Veterinary Sciences that are not published or not being considered for publication elsewhere.
Epidemiological Investigation of Gastrointestinal (GI) Parasite at BAPARD Cat...AI Publications
The aim of the study was to investigate the prevalence and associated risk factors of Gastrointestinal (GI) parasite in Cattle through semi-intensive rearing system at BAPARD campus during the period from July 2019 to June, 2020. A total of 156 samples from 39 cattle among these, 29 Bulls and 10 heifers were collected at the time of defecation or just after defecation. Samples were collected every three months of a year to find out the effect of season. All cattle are Holstein crossbred. The collected Feces samples were examined by direct smear method followed by McMaster counting technique under microscope for detection the morphology of egg at BAPARD Parasitology Lab on the day of collection. The overall prevalence of GI parasite was 26.72% and more frequent in more frequent in rainy season (57.14%) followed by summer (28.57%) and winter season (14.29%). The parasitic prevalence load was low in winter season. In endoparasitic infection, the higher prevalence of Paramphistomum spp. (19.05%) was found in rainy season followed by Haemonchus spp. (9.52%); Fasciola spp.(4.76%), Schistosoma spp. (4.76%) and Toxocara spp. (4.76%). In cattle, prevalence of Paramphistomum spp. (15.38%), Schistososma spp (3.85%) and Haemonchus spp. (7.69%) was higher in adult cattle (above 2 year) comparatively growing and young, whereas prevalence of Toxocara spp. (3.33%) was higher in young (1-1.5 year) than growing and adult cattle. In other case Female (27.5%) are slightly higher than male (26.72%) cattle. So, the investigation indicates low prevalence rate of GI parasite at semi-intensive care management system in BAPARD, Gopalganj,Bangladesh.
Hematological Parameters of three Strains of Local Cocks in Northern NigeriaIJEAB
The study was conducted to determine the hematological parameters of three strains of the Nigerian indigenous cocks. A total of 15 sexually matured (14-18 month of age) breeders cocks comprising (5 normal feathered, 5 frizzled feathered and 5 naked neck) were used for the experiment. The study was conducted from October to December 2016 at the Teaching and Research Farm University of Maiduguri. Blood samples were collected from 9 breeder’s cocks which were randomly selected 3 per genotype and used for hematological parameters examination. Hematological examination such as Packed Cell Volume (PCV), Red Blood Cell ( RBC) , Haemoglobin (Hb), White Blood Cell (WBC), Mean Corpuscular Haemoglobin concentration ( MCHC), Mean Corpuscular Haemoglobin (MCH) and Mean Corpuscular Volume ( MCV) showed significant (P<0.05)> 0.05) different between normal feathered and frizzle feathered but there is significant difference ( P< 0.05) with naked necked cock. Neutrophil ( N) showed significant (P<0.05)>0.05) difference between normal feathered and naked neck feathered , fizzle feathered and necked neck respectively but showed significant (P<0.05) difference between fizzle feathered and normal feathered respectively for M and E. the study concluded that variation in the heamatoloical parameters between three strains of local chicken in Nigeria is due to difference in their genetic makeup.
Brucellosis a negative modulator of reproductionBhoj Raj Singh
Brucellsis is an important disease of reproductive system in dairy animals and other livestock. Though it not causes any apparent illness, it causes infertility in both sexes and adult male cattle may develop orchitis, hygromas involving leg joints are a common manifestation of Brucellosis in calves. Once infected, the animal remain lifelong carrier and continue disseminate the disease to susceptible animals and humans. The pathology and pathogenesis of brucellosis though not very apparent is complex as involves modulation of several cytokines in the process of infection and disease progression. The important cytokines affected in brucellosis pathogenesis and persistence include interferon-γ (IFNγ), tumor necrosis factor-α (TNFα), IL-1, IL-2, IL-4, IL-6, IL-10 and IL-12. All the modulated cytokines are also associated with one or other aspect of normal reproduction physiology as increased levels of IFNγ had strong association with infertility, the TNF-α act as key regulators of endometrial IL-13 and IL-15 expression regulating endometrial immune responses and endometriosis. Higher levels of TNF-α, IL-4, IL-6, IFN-γ, MCP-1, sVCAM-1 induces infertility, the IL-1 have a role in ovulation and may be involved in several ovulation-associated dysfunctions, the IL-2 has a significant negative correlation with sperm count, motility, and morphology in males and repeated abortions in females, the increased levels of IL-4 have been associated with infertility, normalcy in serum levels of the IL-6 important for normal fertility and pregnancy maintenance and increased level of IL-10 and IFN-γ has been associated with reproductive failure.
The molecular approach reveals the relationship among Venus clams (Meretrix s...AbdullaAlAsif1
Molecular study is important to detect variations and similarities among species from the same genus, in case if they do not encompass any morphological or physiological differences. The study was conducted to differentiate among species of Meretrix spp. (Meretrix lyrata, M. meretrix, and M. lusoria) obtained from two locations in Malaysia through the phylogenetic tree. The adductor muscle tissues were used to extract DNA and to perform other procedures; the samples were subjected to analyses using PCR and gel electrophoresis. The multiple sequence comparison was conducted by MUSCLE and the phylogenetic relationships were established using Maximum Likelihood (ML) statistical methods with MEGA 6.0 statistical software. M. lyrata samples showed 99% similarity to the three accessions sequence, where M. lyrata indicated 87% similarities, and M. meretrix showed not more than 89% similarities from the deposited sequence. The nucleotide base composition sequences consisted of the mean of Thiamine (T) 37.9%, Cytosine (C) 15.4%, Adenine (A) 27.4%, and Guanine (G) 19.4%. Maximum Likelihood (ML) analysis was conducted using the Tamura 3-parameter model to establish five major clades on Meretrix spp. and two out-groups clades significantly different from the Meretrix spp. These major clades were closely related to each other at the 50% evidence of bootstrap, which grouped as genus Meretrix. The present study on Meretrix spp. from the Sarawak locality was able to differentiate COI sequences between M. lyrata, M. meretrix, and M. lusoria. M. lusoria was close related to M. meretrix with strong bootstrap supporting evidence at 96% scoring. Moreover, M. lyrata was inferred as the ancestor to M. meretrix, and M. lusoria from Sarawak, Malaysia.
Studies on Prevalence of Ixodid Ticks Infesting Cattle and Their Control by P...iosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
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Reproductive performance of different goat breeds in Malaysia
1. AGRICULTURAL RESEARCH COMMUNICATION CENTRE
www.arccjournals.com/www.ijaronline.in
*Corresponding author’s e-mail: haniza@fsmt.upsi.edu.my
Indian J. Anim. Res., 53(1) 2019: 24-27
Print ISSN:0367-6722 / Online ISSN:0976-0555
Reproductive performance of different goat breeds in Malaysia
Mohammed Muayad TA, Haniza M.Z.H.* and Husni I.
Department of Biology, Faculty of Science and Mathematics,
University Pendidikan Sultan Idris, 35900 Tanjong Malim, Perak, Malaysia.
Received: 12-08-2016 Accepted: 18-10-2016 DOI: 10.18805/ijar.v0iOF.7002
ABSTRACT
The study was conducted to investigate the effect of a controlled internal drug release (CIDR) device in three goat breeds
of Malaysia using Artificial Insemination (AI), on the reproductive performance, which included; Estrus Synchronization
(ES), pregnancy, singleton, twin, and mortality rate. Sixty goats were divided in to treatment group (TRE: n = 30) which
received CIDR for 9 days and AI was done on 10th
day, and a control group (CON: N = 30). Three goat breed, namely,
Kambing Katjang (KK), Boer and Jamnapari, (n = 20 to each strain), were used in this study. . . Data were analyzed to
determine the effects of CIDR and strain on the reproductive performance of goats. ANOVA or T-test were used to analyze
data for the effects ofCIDRand breed. Finding showed the using ofCIDRand breed mayeffect of reproductive performance.
Results indicated that the CIDR can be used to activate the reproductive system, accelerate estrus cycle, and increase twin
rates. Boer strain that might help to improve herd production by producing twins.
Key words: Boer, CIDR, Goats, Jamnapari, Kambing Katjang, Reproductive performance.
Raising high-qualitygoat breeds mainlydetermines
the success of commercial goat production. As such, a
number of exotic goat breeds are imported and bred locally
to satisfy the demand of the industry (Ariff et al., 2010).
The level of reproductive performance depends on
the interaction of genetic and environmental factors (Riera,
1982). Goats are considered to be the most fertile among
domesticated ruminants under tropical conditions and are
able to breed throughout the year (Mamabolo and Webb,
2005). Generally, goats exhibit distinctive seasonal patterns
of reproductive activity in the temperate region. In tropical
regions, the breeding period of goats spans throughout the
year and is dependent on latitude, climate, food availability,
breed, and breeding system (Khan et al., 2008).
Different reproductive technologies have been
developed to address the increasing demand for animal
products (Gordon, 2005).
The length of cycle and duration of estrus is highly
variable in goats (Romano, 1993), therefore, synchronization
of ovulation time is essential to ensure acceptable
reproductive rates during fixed-time artificial insemination
(Fierro et al., 2013) and to achieve this intravaginal devices
impregnated with progesterone or other progestagens have
been used (Abecia et al., 2012).
Use of AI and estrus synchronization reduce the
cost of maintaining bucks, improve germplasm and
several does can be inseminated at a time (Chris and Robert
2014).
Therefore, the present study was planned to
evaluate the effectiveness of CIDR device on reproductive
performance in three goat breeds of Malaysia.
Animals and location of experiments: Sixty goats of three
indigenous breeds, KK, Boer and Jamnapari maintained at
Alhilmi Farm, Slim River, Perak, Malaysia under the
supervision of the Biology Department, Faculty of Science
and Mathematics, University Pendidikan Sultan Idris were
used. Before start of experiment, the animals were fed and
handled by research workers for two weeks to avoid any
kind of stress. Nine bucks were also maintained at the same
farm and their semen was used for AI of the treated females
and liquid semen was used for insemination of control
animals
Experimental design: Twenty goats from each breed were
randomly selected (n=60) and these were divided in to
treatment group (n=30; ten animals of each breed) and
control group (n=30; ten animals ofeach breed). The females
of the treatment group received CIDR containing 0.3 g of
progesterone, inserted in vagina and kept in situ for 9 days
whereas the control group animals did not receive any
treatment. Animals of both the groups were monitored for
the occurrence of estrus based on the appearance of estrus
signs, including vaginal secretions, vagina lining congestion,
vulva swelling (Bearden et al., 2004).
Artificial insemination (AI): In treated goats AI was done
24 h after CIDR removal, whereas the animals of control
groups which exhibited estrus were inseminated with diluted
2. Volume 53 Issue 1 (January 2019) 25
semen containing 300 × 106
spermatozoa, 24 h after estrus
detection.
Repeat the experiments: The experiment was conducted
in March 2014 then repeated one-year later (in March 2015)
Statistical analysis: The data was subjected to statistical
analysis using SPSS for analyzing data among groups.
Individual parameters were assessed byT-Test andANOVA
todetermine the effects ofCIDRand breed variations. When
significant differences were detected, Tukey’s post-hoc
follow-up test or Dunnett’s test was employed to detect
significant differences within or among groups, respectively.
RESULTS AND DISCUSSION
Estrus synchronization: In the present study a high
percentage of treated females (90%) exhibited heat signs
within 24 h after CIDR removal (Table 1) Similar estrus
response was reported by Omontese et al. (2013) and the
results are in consistent with the findings of. Leboeuf et al.,
(2003) and Freitas et al., (2004) whotreated goats with CIDR
under temperate and tropical conditions respectively.
In the present experiment about 70 % of the treated
goats conceived which is in agreement with the findings of
Motlomelo et al. (2002) who treated Boer goats with CIDR,
however the pregnancy rates were slightlyhigher than those
reported by; Leboeuf et al. (2008) who recorded 65%
pregnancy rate and it may be attributed to use of cervical
insemination.
Singleton rate: In the present study, singleton kidding rate
was not significantly affected and a similar reproductive
performance was reported in Sudanese Nubian goats
synchronized with intravaginal sponges or cloprostenol by
Ahmed et al., (1998). Similar tothat of present low singleton
in Boer, Greyling (2000), alsoreported a significantlylower
singletons rate in this breed (Table 2).
Twin rate: In the present study, a higher twin rate was
observed in treated females and in Boer strain. This result
ofthe present studywas contrarytothe findings ofNasroallah
et al. (2011), who concluded that kidding rate was not
significantlyaffected by synchronization methods. However,
similar results were also reported byThatcher et al., (2001);
Safdarian et al., (2006). and it has been observed that under
intensive conditions, Boer goat did successfullyraise twins
(Naude and Hofmeyr, 1981).
Mortality rate: Results of overall mortality rate in present
study were higher than those of Sabapara and Deshpande,
(2010); Dohare et al., (2013), who recorded overall mortality
rates of 8.42% and 10.20%, respectively, in goats under field
conditions.
The data analysis showed that the mortality was
highest (15.87%) during 0-3 months ofage and subsequently
it declined (Table 3). This might be due to less immunity in
kids, which leads to disease and ultimately mortality.
However, the incidence waslower than that reported bySingh
et al. (2009) and Dohare et al. (2013), whorecorded 39.29%
mortilityduring 0-3 months of age. A 12.7% mortality rate
observed in Jamnapari goats was lower thanthat reported by
Singh et al. (2009).
Table 1: Effect of treatment with CIDR on the reproductive efficiency of goats.
Treatment ES Pregnancy singleton Twin
TRE (n=30) 0.9 ± 0.305 a
0.73 ± 0.449 a
0.5 ± 0.508 a
0.35 ± 0.430 a
CON (n=30) 0 ± 0.000 0.73 ± 0.449 a
0.67 ± 0.479 a
0.05 ± 0.182 b
Values in the same column with different letters differ (P < 0.05).
Table 2: Effect of breed variation on the reproductive efficiency of goats.
Strain ES Pregnancy singleton Twin
KK (n=20) 0.9 ± 0.510 a
0.7 ± 0.470 a
0.7 ± 0.470 a
0 ± 0.000 c
Boer (n=20) 1.0 ± 0.513 a
0.75 ± 0.444 a
0.4 ± 0.502 b
0.3 ± 0.470 a
Jamnapari(n=20) 0.8 ± 0.503 a
0.75 ± 0.444 a
0.65 ± 0.489 a
0.1 ± 0.308 b
Values in the same column with different letters differ (P < 0.05).
Table 3: Kids’ mortality of different breeds, ages and sex.
Breed New Birth Singleton Twin
Male Female
1st
day 1st
month 3rd
month 5th
month 1st
day 1st
month 3rd
month 5th
month
KK 24 2 1 0 0 1 0 0 1 0
Boer 18 0 0 0 0 0 0 0 0 0
Jamnapari 21 1 2 1 1 2 0 0 1 0
Total 63 3 a
3 a
1 c
1 c
3 a
0 d
0 d
2 b
0
Values in the same row with different letters differ (P < 0.05).
3. 26 INDIAN JOURNAL OF ANIMAL RESEARCH
The mortality rate in present study was affected
by sex and similarly Debele et al. (2011) and Dohare et
al. (2013) recorded a higher mortality rate in females than
males.
CONCLUSION
The results indicate that a 9-day treatment with
intravaginal CIDRs can efficiently control and
synchronize the estrous cycle with improving reproductive
performance of goats in Malaysia, particularly in Boer
goats and this breed can be utilized even in more intensive
production systems for animal productions.
ACKNOWLEDGEMENT
We would like to acknowledge the invaluable
contribution of Alhilmi Farm, Slim River, Perak, Malaysia
and the Biology Department, Faculty of Science and
Mathematics, University Pendidikan Sultan Idris in
accomplishing this study.
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