This study examined the effects of desloratadine on ovarian ischemia-reperfusion injury in rats. Rats were divided into three groups: an ischemia-reperfusion injury group, an ischemia-reperfusion injury group treated with desloratadine, and a sham group. Ovarian tissue was analyzed for markers of oxidative stress and inflammation after ischemia and reperfusion. Results showed that desloratadine significantly reduced oxidative stress markers like MDA and increased antioxidant markers like GSH compared to the ischemia-reperfusion injury group. Desloratadine also decreased levels of proinflammatory cytokines like NF-κB, IL-1β, and TNF-α. Histological analysis revealed that desl
Objective: To investigate the effect of sildenafil on reducing the impact of hepatic ischemia/reperfusion (HIR) injury established by Pringle maneuver on the heart of rats.
Study Design: Forty Wistar albino rats were divided into 4 groups: Sham (laparotomy only), Control (laparotomy following sildenafil application), IR (ischemia/reperfusion injured by HIR), and IR+SIL (injured by HIR following sildenafil application). Ischemia was developed by clamping the hepatoduodenal ligament for 30 minutes; then reperfusion was applied for 30 minutes. Sildenafil (single dose of 50 mg/kg) was administered by oral gavage for 15 minutes before ischemia. Blood samples of rats were collected from Sham and Control groups at 60 minutes and from IR and IR+SIL groups at 30 minutes after initiation of reperfusion for biochemical analysis. Meanwhile, heart tissues were sampled for biochemical analysis. Malondialdehyde (MDA) and total antioxidant capacity (TAC) in serum samples and TAC, total oxidative capacity (TOC), and oxidative stress index in heart tissues were examined biochemically.
Results: Serum MDA levels were elevated significantly in the IR and IR+SIL groups as compared to the sham group. Sildenafil treatment inhibited MDA increase considerably in the IR+SIL group as compared to the IR group. Serum TAC levels were elevated significantly in the sildenafil and control groups (compared with sham groups) and in the IR+SIL group (compared with the IR group). TAC levels detected in heart tissue increased significantly in the IR group as compared to the sham group; however, sildenafil treatment had no effect on this increase.
Conclusion: Heart tissue was affected by HIR. It was revealed that sildenafil treatment may prevent the oxidative stress via increasing serum TAC levels in both control and IR+SIL groups.
Objective: To investigate the immunohistochemical staining of hypoxia-inducible factor 1-alpha (HIF-1α) and Ki-67 expression in the placenta of pregnant women with placenta previa and placenta accreta.
Study Design: Thirty placentas (10 normotensive, 10 placenta previa, and 10 placenta accreta) were processed for routine histological tissue processing. The biochemical parameters of patients were recorded. Placentas were stained with hematoxylin-eosin and HIF-1α and Ki-67 immunostaining.
Results: Normal histology was observed in placentas of normotensive pregnant women. Placenta previa sections showed increased syncytial knots, intervillous hemorrhage, fibrin accumulation, and hyalinization. In placenta accreta sections, increased syncytial nodes, vascular dilation/congestion, fibrin accumulation, and hyalinization were observed. Normotensive placentas showed no HIF-1α expression. In placenta previa tissues, high HIF-1α expression was observed in vascular endothelial cells, villous stromal cells, and syncytial knots. High HIF-1α expression was recorded in villous stromal cells and cytotrophoblast cells in placenta accreta. In normotensive placental tissues, no Ki-67 expression was observed. In placenta previa sections, high Ki-67 expression was observed mostly in root villi stromal cells and some endothelial cells. High Ki-67 expression was observed mostly in villi stromal cells of placenta accreta.
Conclusion: It is thought that HIF-1α is an important regulatory gene in the development of villus in trophoblast invasion such as placenta accreta and previa, while Ki-67 will play a key role in the development of abnormal placenta with its stimulating effect on inflammatory cell development and angiogenesis in accreta and preeclampsia.
Objective: To investigate the protective effect of lo- sartan, an angiotensin II type 1 receptor blocker with antioxidative effect on intestinal ischemia-reperfusion (I/R) injury in rats, against inflammation and apoptotic development.
Study Design: Forty male Wistar albino rats with a mean weight of 200–250 g each were divided into 4 groups: (1) Sham operation (laparotomy only, sham surgical preparation including isolation of the superior mesenteric artery [SMA] without occlusion), (2) Ischemia model with SMA closure for 2 hours, (3) I/R group (2 hours of ischemia followed by 3-hour reperfusion (SMA occlusion for 120 minutes followed by 240 minutes reperfusion), and (4) Losartan group (2 hours of ischemia, 40 mg/kg losartan was administered to the animals; losartan was dissolved in 1 mL distilled water and administered intraperitoneally after 2 hours of ischemia). Malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) levels were examined in jejunum tissue.
Results: Losartan treatment reduced the I/R-induced increase in MDA levels in the gut. Statistically, while SOD, CAT, and GSH activities decreased significantly in the I/R group, they increased in the I/R+Losartan group. Villus loss and increase in inflammation after ischemia persisted after reperfusion. Losartan treatment played a role in the reduction of inflammation and apoptosis and in the regulation of TNF-α and caspase-9 activity.
Conclusion: It has been thought that losartan in I/R may reduce mucosal damage and cell apoptosis in the direction of inflammation and may stabilize caspase-9 activity by inhibiting TNF-α stimulus.
Keywords: caspase-9, ischemia, ischemia/reperfusion, rat, reperfusion injury, TNF-α, tumor necrosis factor-alpha
Objective: A spinal cord injury (SCI) is damage to the spinal cord either from trauma, loss of its normal blood supply, or compression from tumor or infection. In this study we focused on alterations in the bladder tissue with angiogenic and apoptotic aspects after spinal cord injury.
Study Design: Twenty Wistar Albino rats were categorized as control and SCI groups. At T7-T9 vertebras, a steel rod was dropped from 10 cm to create a spinal cord injury under anesthesia. Rats were decapitated and spinal tissue was processed to measure malondialdehyde (MDA), glutathione (GSH), and myeloperoxidase (MPO).
Results: MDA, MPO, epithelial degeneration, vascular dilation, inflammation, VEGF, and APAF-1 expressions in the SCI group were statistically higher than those in the control group. GSH content of the SCI group was statistically lower than that in the control group. In the hematoxylin-eosin–stained sections of the control group, normal histology was observed in bladder tissue. In the SCI group, degeneration epithelial cells, thinned epithelium, increased fibrosis, dilated and congested blood vessels, and hyperplastic endothelial cells were observed. In the control group, VEGF expression was slightly observed in some epithelial cells and vascular cells. In the SCI group, VEGF expression was increased in inflammatory and vascular endothelial cells. For APAF-1 expression, the control group showed no expression. In the SCI group, APAF-1 expression was positive in degenerated epithelial cells and connective tissue cells.
Conclusion: It is thought that the urination reflex was affected due to increased inflammation in the bladder tissue, leading to alterations in the regulation and function of the muscles.
Objective: To investigate the effect of sildenafil on reducing the impact of hepatic ischemia/reperfusion (HIR) injury established by Pringle maneuver on the heart of rats.
Study Design: Forty Wistar albino rats were divided into 4 groups: Sham (laparotomy only), Control (laparotomy following sildenafil application), IR (ischemia/reperfusion injured by HIR), and IR+SIL (injured by HIR following sildenafil application). Ischemia was developed by clamping the hepatoduodenal ligament for 30 minutes; then reperfusion was applied for 30 minutes. Sildenafil (single dose of 50 mg/kg) was administered by oral gavage for 15 minutes before ischemia. Blood samples of rats were collected from Sham and Control groups at 60 minutes and from IR and IR+SIL groups at 30 minutes after initiation of reperfusion for biochemical analysis. Meanwhile, heart tissues were sampled for biochemical analysis. Malondialdehyde (MDA) and total antioxidant capacity (TAC) in serum samples and TAC, total oxidative capacity (TOC), and oxidative stress index in heart tissues were examined biochemically.
Results: Serum MDA levels were elevated significantly in the IR and IR+SIL groups as compared to the sham group. Sildenafil treatment inhibited MDA increase considerably in the IR+SIL group as compared to the IR group. Serum TAC levels were elevated significantly in the sildenafil and control groups (compared with sham groups) and in the IR+SIL group (compared with the IR group). TAC levels detected in heart tissue increased significantly in the IR group as compared to the sham group; however, sildenafil treatment had no effect on this increase.
Conclusion: Heart tissue was affected by HIR. It was revealed that sildenafil treatment may prevent the oxidative stress via increasing serum TAC levels in both control and IR+SIL groups.
Objective: To investigate the immunohistochemical staining of hypoxia-inducible factor 1-alpha (HIF-1α) and Ki-67 expression in the placenta of pregnant women with placenta previa and placenta accreta.
Study Design: Thirty placentas (10 normotensive, 10 placenta previa, and 10 placenta accreta) were processed for routine histological tissue processing. The biochemical parameters of patients were recorded. Placentas were stained with hematoxylin-eosin and HIF-1α and Ki-67 immunostaining.
Results: Normal histology was observed in placentas of normotensive pregnant women. Placenta previa sections showed increased syncytial knots, intervillous hemorrhage, fibrin accumulation, and hyalinization. In placenta accreta sections, increased syncytial nodes, vascular dilation/congestion, fibrin accumulation, and hyalinization were observed. Normotensive placentas showed no HIF-1α expression. In placenta previa tissues, high HIF-1α expression was observed in vascular endothelial cells, villous stromal cells, and syncytial knots. High HIF-1α expression was recorded in villous stromal cells and cytotrophoblast cells in placenta accreta. In normotensive placental tissues, no Ki-67 expression was observed. In placenta previa sections, high Ki-67 expression was observed mostly in root villi stromal cells and some endothelial cells. High Ki-67 expression was observed mostly in villi stromal cells of placenta accreta.
Conclusion: It is thought that HIF-1α is an important regulatory gene in the development of villus in trophoblast invasion such as placenta accreta and previa, while Ki-67 will play a key role in the development of abnormal placenta with its stimulating effect on inflammatory cell development and angiogenesis in accreta and preeclampsia.
Objective: To investigate the protective effect of lo- sartan, an angiotensin II type 1 receptor blocker with antioxidative effect on intestinal ischemia-reperfusion (I/R) injury in rats, against inflammation and apoptotic development.
Study Design: Forty male Wistar albino rats with a mean weight of 200–250 g each were divided into 4 groups: (1) Sham operation (laparotomy only, sham surgical preparation including isolation of the superior mesenteric artery [SMA] without occlusion), (2) Ischemia model with SMA closure for 2 hours, (3) I/R group (2 hours of ischemia followed by 3-hour reperfusion (SMA occlusion for 120 minutes followed by 240 minutes reperfusion), and (4) Losartan group (2 hours of ischemia, 40 mg/kg losartan was administered to the animals; losartan was dissolved in 1 mL distilled water and administered intraperitoneally after 2 hours of ischemia). Malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) levels were examined in jejunum tissue.
Results: Losartan treatment reduced the I/R-induced increase in MDA levels in the gut. Statistically, while SOD, CAT, and GSH activities decreased significantly in the I/R group, they increased in the I/R+Losartan group. Villus loss and increase in inflammation after ischemia persisted after reperfusion. Losartan treatment played a role in the reduction of inflammation and apoptosis and in the regulation of TNF-α and caspase-9 activity.
Conclusion: It has been thought that losartan in I/R may reduce mucosal damage and cell apoptosis in the direction of inflammation and may stabilize caspase-9 activity by inhibiting TNF-α stimulus.
Keywords: caspase-9, ischemia, ischemia/reperfusion, rat, reperfusion injury, TNF-α, tumor necrosis factor-alpha
Objective: A spinal cord injury (SCI) is damage to the spinal cord either from trauma, loss of its normal blood supply, or compression from tumor or infection. In this study we focused on alterations in the bladder tissue with angiogenic and apoptotic aspects after spinal cord injury.
Study Design: Twenty Wistar Albino rats were categorized as control and SCI groups. At T7-T9 vertebras, a steel rod was dropped from 10 cm to create a spinal cord injury under anesthesia. Rats were decapitated and spinal tissue was processed to measure malondialdehyde (MDA), glutathione (GSH), and myeloperoxidase (MPO).
Results: MDA, MPO, epithelial degeneration, vascular dilation, inflammation, VEGF, and APAF-1 expressions in the SCI group were statistically higher than those in the control group. GSH content of the SCI group was statistically lower than that in the control group. In the hematoxylin-eosin–stained sections of the control group, normal histology was observed in bladder tissue. In the SCI group, degeneration epithelial cells, thinned epithelium, increased fibrosis, dilated and congested blood vessels, and hyperplastic endothelial cells were observed. In the control group, VEGF expression was slightly observed in some epithelial cells and vascular cells. In the SCI group, VEGF expression was increased in inflammatory and vascular endothelial cells. For APAF-1 expression, the control group showed no expression. In the SCI group, APAF-1 expression was positive in degenerated epithelial cells and connective tissue cells.
Conclusion: It is thought that the urination reflex was affected due to increased inflammation in the bladder tissue, leading to alterations in the regulation and function of the muscles.
Objective: To study the effects of resveratrol in neuronal structures in traumatic brain injury (TBI).
Study Design: Thirty rats were categorized as (1) control group (n=10), saline solution administered i.p. for 14 days, (2) TBI group (n=10), trauma induced by weight-drop model on brain, and (3) TBI+Resveratrol group (n=10), 15 minutes after injury the rats were given resveratrol (10 μmoL/kg/i.p.) for 14 days. At the end of the experiment the cerebellum was excised for routine paraffin tissue protocol. Blood samples were tested for serum biochemical markers (MDA, SOD, CAT, and GSH-x).
Results: SOD, GPx, and CAT values were lowest in the TBI group. MDA and histological scores of dilations in vessels, inflammation, degeneration in neurons, apoptosis in microglia, ADAMTS8, and GFAP expressions were highest in the TBI group. Sections of the control group showed normal cerebellar histology. The trauma group showed degenerated ganglion layer, pyknotic and apoptotic Purkinje cell nuclei. Vascular thrombus was seen in the substantia alba and substantia grisea. In the Trauma+Resveratrol group, most pa- thologies observed in the TBI group were improved. In the control group, GFAP protein was expressed in granular cells, axons, dendrites, Purkinje cells, and microglia cells. In the trauma group, increased GFAP expression was observed in glial processes, neurons, and Purkinje cells. In the Trauma+Resveratrol group, GFAP was expressed in molecular layer and glial processes. In the control group, ADAMTS-4 activity was observed in granulosa layer, glial cells, and Purkinje cells. In the trauma group, ADAMTS-4 expression was positive in Purkinje cells and glial cells. In the Trauma+ Resveratrol group, ADAMTS-4 was expressed in Purkinje cells, granular cells, and glial cells.
Conclusion: GFAP and ADAMTS-4 proteins may be involved in regeneration of damaged astroglial cells and other glial cells, Purkinje cells, and synaptic extensions. We suggest that antioxidative drugs such as resveratrol may be alternative target agents in neurological disease.
Keywords: ADAMTS-4, brain, cerebellum, GFAP, rat, resveratrol, traumatic brain injury
ABSTRACT
Background:The main objective of the study is to determine the anti-arthritic effect of whole plant ethanolic extract of Polygonum glabrum
belonging to the family Polygonaceae in Female wistar rats using the Freund’s Complete Adjuvant (FCA) model . Methods:The plants areal
parts were collected near Tirupathi hills, Chittoor district of Andhra Pradesh in India. The Phytoconstituents were identified through the
chemical tests. Ethanol (95%) was used to obtain the whole plant extraction through Soxhlet extractor. Female SD rats were used for antiarthritic
screening. Arthritis was induced using FCA, and the anti-arthritic effect of the ethanolic extract of P.glabrum was studied at doses
of 250 and500 mg/kg. The effects were compared with those of indomethacin (10 mg/kg). At the end of the study, theliver enzyme levels were
determined and a radiological examination was carried out. Results and Discussion:The preliminary phytochemical analysis of the ethanolic
extract of Polygonum glabrum showed the presence of alkaloids, tannins, flavonoids and saponins. P. glabrum at 250 and 500 mg/kg
significantly inhibited the FCA-induced arthritis in the rats. This was manifested by as a decrease in the paw volume. The arthritic control
animals exhibited a significant decrease in body weight compared with control animals without arthritis. P. glabrum treated animals showed
dose dependent reduction in decrease in body weight and arthritis.At the same time, P.glabrum significantly altered the biochemical and
haematological changes induced by FCA (P < 0.05). The anti-arthritic effect of P.glabrum was comparable with that of Indomethacin.
Conclusion:The whole plant extract of P.glabrum showed significant anti-arthritic activity against FCA-induced arthritis in female Wistar
rats.
Objective: To evaluate the results of the effect of nebivolol on tibial bone defect and graft application in new bone development in the rat.
Study Design: Thirty Wistar albino rats were divided into 3 groups. In the Control group, tibia bone defect was created without any treatment. In the Defect+ Graft group, allograft treatment was performed by forming a 6 mm tibial bone defect. In the Defect+Graft+ Nebivolol group, alloplastic bone graft was placed in the calvarial bone defect and then nebivolol (0.34 mg/mL solution/day) treatment was intraperitoneally applied for 28 days.
Results: Histopathological examination revealed inflammation in the defect area, congestion in the vessels, degeneration in collagen fibers, and an increase in osteoclast cells. There was an increase in inflammation and blood vessel structure in graft application, and osteoblastic activity matrix formation after reorganization nebivolol application in collagen fibers. Osteonectin expression was positive in the collagen fiber and matrix, starting in the Graft group, in osteoblasts, whereas in the Nebivolol group, osteoblasts increased in osteocytes and new bone formation.
Conclusion: Nebivolol is thought to have a positive effect on osteoinductive bone growth factors and contribute to the cell-matrix interaction, in addition to the supporting effect of the graft with its antioxidative effect.
Keywords: allograft; bone; bone regeneration; disease models, animal; nebivolol; orthopedic procedures; osteonectin; rats; tibia; tibial defect
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
The Ameliorative Potential of Dexmedetomidine and Benincasa Cerifera Extract ...Prof. Hesham N. Mustafa
Renal ischemia/reperfusion injury (IRI) represents the main reason for acute kidney injury (AKI). Dexmedetomidine (Dex) and Benincasa cerifera (BC) have wide benefits due to their anti-inflammatory and antioxidant properties. This study aims to illustrate the protective effects of BC and Dex on renal IRI in a diabetic model. Sixty adult male albino rats (Wistar strain), weighing 250–300 g, were included in the study. The rats were divided into four groups, as follows: sham group: (non-diabetic); diabetes mellitus (DM) + IRI group: streptozotocin (STZ)-induced diabetic rats exposed to renal IRI on day 30 after diagnosis of diabetes; DM + IRI + BC group: STZ-induced diabetic rats treated with BC (500 mg/kg) for 30 days after diagnosis of diabetes, then exposed to renal IRI; and DM + IRI + Dex group: STZ-induced diabetic rats treated with Dex (100 µg/kg intraperitoneally) 5 min before induction of ischemia on day 30 after diagnosis of diabetes, then exposed to renal IRI. Biochemical parameters, histopathological examination, and immunohistochemical markers were evaluated. A significant improvement in the biochemical, histopathological, and immunohistochemical parameters were observed in the DM + IRI + BC group, while the DM + IRI + Dex group showed improvements in renal IRI and dyslipidemia. The present study demonstrated that oxidative stress plays a chief role in renal IRI in the STZ-induced diabetic model. Treatment with BC achieved excellent ameliorative effects, while treatment with DEX improved renal IRI.
Keywords:
Diabetes; Dexmedetomidine; Ischemia/Reperfusion; Oxidative Stress
Sexual behavior and fertility of male rats following subacute hemi-orchidectomyInnspub Net
This study was conducted to investigate the sub-acute response of testicular size, body weight, fertility and mating indices in hemi-orchiectomy induced suppression of testosterone levels (<0.5 ng/mL) among adult male Wistar rats (Four months old). Libido, fertility and extended implication of the procedure on F1 generation were also observed. After exposure to relevant experimental procedure, each of the male rats was housed with a proven female breeder to assess mating and fertility indices. Obtained data was statistically analyzed by analysis of variance. Hemi-orchidectomy resulted in compensatory increase in testosterone secretion due to contralateral testicular hypertrophy, mating and fertility indices of hemi-orchidectomized and fully orchidectomized rats were 80%:60% and 20%:0% respectively. The male progeny belonging to F1 generation showed no sign of infertility. The findings suggest that hemi-orchidectomy induces sudden compensatory testicular hypertrophy, immediate Loss of fertility after full orchidectomy, diminished libido rather than complete loss of libido occurs. In conclusion, number of offspring delivery is independent of testosterone level, however testosterone level and its interference with the complex modifications in the hypothalamo-hypophyseal secretions are involved in the central control of male sexual activity.
Biochemical changes induced by Bioneem (0.03%) formulation in chick embryogen...Agriculture Journal IJOEAR
Abstract— In ovo studies on the effect of 1,3,5, ppm Bioneem (0.03%) formulation on Biochemical aspect of chick embryo revealed that there was dose dependent total protein reduction in 96 hrs old embryo (treated at 24 hrs) as compared to the control. Also there was reduction in total protein concentration Liver, Brain and Heart of 15 day old chick embryo (treated with Bioneem at 96 hrs. stage) as compared to that of control. Protein carbonyl concentration of 96 hrs old embryo (treated at 24 hrs with Bioneem) and that of Liver, Brain and Heart of 15 day old chick embryo (treated with bioneem at 96 hrs) increased in dose dependent manner. Most affected organ was Liver and least affected organ was Heart. Blood analysis of 15 day old chick embryo (treated with Bioneem at 96 hrs) showed increased level of Blood urea, LDH, SGOT, SGPT, while Serum alkaline phosphatase and serum cholesterol were decreased in dose dependent manner as compared to the control. Thus Bioneem though ecofriendly pesticide can adversely affect vertebrate non target organisms and therefore should be carefully used in pest management programs.
Ciprofloxacin resideu and their impact on Biomolecules n eggs of laying hens ...Sayed Koushik Ahamed
I have done this research on eggs for the welfare of mankind now i want to share my article for social awareness. I hope it will helps all researchers for their future further research.
Thank You
Objective: To study the effects of resveratrol in neuronal structures in traumatic brain injury (TBI).
Study Design: Thirty rats were categorized as (1) control group (n=10), saline solution administered i.p. for 14 days, (2) TBI group (n=10), trauma induced by weight-drop model on brain, and (3) TBI+Resveratrol group (n=10), 15 minutes after injury the rats were given resveratrol (10 μmoL/kg/i.p.) for 14 days. At the end of the experiment the cerebellum was excised for routine paraffin tissue protocol. Blood samples were tested for serum biochemical markers (MDA, SOD, CAT, and GSH-x).
Results: SOD, GPx, and CAT values were lowest in the TBI group. MDA and histological scores of dilations in vessels, inflammation, degeneration in neurons, apoptosis in microglia, ADAMTS8, and GFAP expressions were highest in the TBI group. Sections of the control group showed normal cerebellar histology. The trauma group showed degenerated ganglion layer, pyknotic and apoptotic Purkinje cell nuclei. Vascular thrombus was seen in the substantia alba and substantia grisea. In the Trauma+Resveratrol group, most pa- thologies observed in the TBI group were improved. In the control group, GFAP protein was expressed in granular cells, axons, dendrites, Purkinje cells, and microglia cells. In the trauma group, increased GFAP expression was observed in glial processes, neurons, and Purkinje cells. In the Trauma+Resveratrol group, GFAP was expressed in molecular layer and glial processes. In the control group, ADAMTS-4 activity was observed in granulosa layer, glial cells, and Purkinje cells. In the trauma group, ADAMTS-4 expression was positive in Purkinje cells and glial cells. In the Trauma+ Resveratrol group, ADAMTS-4 was expressed in Purkinje cells, granular cells, and glial cells.
Conclusion: GFAP and ADAMTS-4 proteins may be involved in regeneration of damaged astroglial cells and other glial cells, Purkinje cells, and synaptic extensions. We suggest that antioxidative drugs such as resveratrol may be alternative target agents in neurological disease.
Keywords: ADAMTS-4, brain, cerebellum, GFAP, rat, resveratrol, traumatic brain injury
ABSTRACT
Background:The main objective of the study is to determine the anti-arthritic effect of whole plant ethanolic extract of Polygonum glabrum
belonging to the family Polygonaceae in Female wistar rats using the Freund’s Complete Adjuvant (FCA) model . Methods:The plants areal
parts were collected near Tirupathi hills, Chittoor district of Andhra Pradesh in India. The Phytoconstituents were identified through the
chemical tests. Ethanol (95%) was used to obtain the whole plant extraction through Soxhlet extractor. Female SD rats were used for antiarthritic
screening. Arthritis was induced using FCA, and the anti-arthritic effect of the ethanolic extract of P.glabrum was studied at doses
of 250 and500 mg/kg. The effects were compared with those of indomethacin (10 mg/kg). At the end of the study, theliver enzyme levels were
determined and a radiological examination was carried out. Results and Discussion:The preliminary phytochemical analysis of the ethanolic
extract of Polygonum glabrum showed the presence of alkaloids, tannins, flavonoids and saponins. P. glabrum at 250 and 500 mg/kg
significantly inhibited the FCA-induced arthritis in the rats. This was manifested by as a decrease in the paw volume. The arthritic control
animals exhibited a significant decrease in body weight compared with control animals without arthritis. P. glabrum treated animals showed
dose dependent reduction in decrease in body weight and arthritis.At the same time, P.glabrum significantly altered the biochemical and
haematological changes induced by FCA (P < 0.05). The anti-arthritic effect of P.glabrum was comparable with that of Indomethacin.
Conclusion:The whole plant extract of P.glabrum showed significant anti-arthritic activity against FCA-induced arthritis in female Wistar
rats.
Objective: To evaluate the results of the effect of nebivolol on tibial bone defect and graft application in new bone development in the rat.
Study Design: Thirty Wistar albino rats were divided into 3 groups. In the Control group, tibia bone defect was created without any treatment. In the Defect+ Graft group, allograft treatment was performed by forming a 6 mm tibial bone defect. In the Defect+Graft+ Nebivolol group, alloplastic bone graft was placed in the calvarial bone defect and then nebivolol (0.34 mg/mL solution/day) treatment was intraperitoneally applied for 28 days.
Results: Histopathological examination revealed inflammation in the defect area, congestion in the vessels, degeneration in collagen fibers, and an increase in osteoclast cells. There was an increase in inflammation and blood vessel structure in graft application, and osteoblastic activity matrix formation after reorganization nebivolol application in collagen fibers. Osteonectin expression was positive in the collagen fiber and matrix, starting in the Graft group, in osteoblasts, whereas in the Nebivolol group, osteoblasts increased in osteocytes and new bone formation.
Conclusion: Nebivolol is thought to have a positive effect on osteoinductive bone growth factors and contribute to the cell-matrix interaction, in addition to the supporting effect of the graft with its antioxidative effect.
Keywords: allograft; bone; bone regeneration; disease models, animal; nebivolol; orthopedic procedures; osteonectin; rats; tibia; tibial defect
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
The Ameliorative Potential of Dexmedetomidine and Benincasa Cerifera Extract ...Prof. Hesham N. Mustafa
Renal ischemia/reperfusion injury (IRI) represents the main reason for acute kidney injury (AKI). Dexmedetomidine (Dex) and Benincasa cerifera (BC) have wide benefits due to their anti-inflammatory and antioxidant properties. This study aims to illustrate the protective effects of BC and Dex on renal IRI in a diabetic model. Sixty adult male albino rats (Wistar strain), weighing 250–300 g, were included in the study. The rats were divided into four groups, as follows: sham group: (non-diabetic); diabetes mellitus (DM) + IRI group: streptozotocin (STZ)-induced diabetic rats exposed to renal IRI on day 30 after diagnosis of diabetes; DM + IRI + BC group: STZ-induced diabetic rats treated with BC (500 mg/kg) for 30 days after diagnosis of diabetes, then exposed to renal IRI; and DM + IRI + Dex group: STZ-induced diabetic rats treated with Dex (100 µg/kg intraperitoneally) 5 min before induction of ischemia on day 30 after diagnosis of diabetes, then exposed to renal IRI. Biochemical parameters, histopathological examination, and immunohistochemical markers were evaluated. A significant improvement in the biochemical, histopathological, and immunohistochemical parameters were observed in the DM + IRI + BC group, while the DM + IRI + Dex group showed improvements in renal IRI and dyslipidemia. The present study demonstrated that oxidative stress plays a chief role in renal IRI in the STZ-induced diabetic model. Treatment with BC achieved excellent ameliorative effects, while treatment with DEX improved renal IRI.
Keywords:
Diabetes; Dexmedetomidine; Ischemia/Reperfusion; Oxidative Stress
Sexual behavior and fertility of male rats following subacute hemi-orchidectomyInnspub Net
This study was conducted to investigate the sub-acute response of testicular size, body weight, fertility and mating indices in hemi-orchiectomy induced suppression of testosterone levels (<0.5 ng/mL) among adult male Wistar rats (Four months old). Libido, fertility and extended implication of the procedure on F1 generation were also observed. After exposure to relevant experimental procedure, each of the male rats was housed with a proven female breeder to assess mating and fertility indices. Obtained data was statistically analyzed by analysis of variance. Hemi-orchidectomy resulted in compensatory increase in testosterone secretion due to contralateral testicular hypertrophy, mating and fertility indices of hemi-orchidectomized and fully orchidectomized rats were 80%:60% and 20%:0% respectively. The male progeny belonging to F1 generation showed no sign of infertility. The findings suggest that hemi-orchidectomy induces sudden compensatory testicular hypertrophy, immediate Loss of fertility after full orchidectomy, diminished libido rather than complete loss of libido occurs. In conclusion, number of offspring delivery is independent of testosterone level, however testosterone level and its interference with the complex modifications in the hypothalamo-hypophyseal secretions are involved in the central control of male sexual activity.
Biochemical changes induced by Bioneem (0.03%) formulation in chick embryogen...Agriculture Journal IJOEAR
Abstract— In ovo studies on the effect of 1,3,5, ppm Bioneem (0.03%) formulation on Biochemical aspect of chick embryo revealed that there was dose dependent total protein reduction in 96 hrs old embryo (treated at 24 hrs) as compared to the control. Also there was reduction in total protein concentration Liver, Brain and Heart of 15 day old chick embryo (treated with Bioneem at 96 hrs. stage) as compared to that of control. Protein carbonyl concentration of 96 hrs old embryo (treated at 24 hrs with Bioneem) and that of Liver, Brain and Heart of 15 day old chick embryo (treated with bioneem at 96 hrs) increased in dose dependent manner. Most affected organ was Liver and least affected organ was Heart. Blood analysis of 15 day old chick embryo (treated with Bioneem at 96 hrs) showed increased level of Blood urea, LDH, SGOT, SGPT, while Serum alkaline phosphatase and serum cholesterol were decreased in dose dependent manner as compared to the control. Thus Bioneem though ecofriendly pesticide can adversely affect vertebrate non target organisms and therefore should be carefully used in pest management programs.
Ciprofloxacin resideu and their impact on Biomolecules n eggs of laying hens ...Sayed Koushik Ahamed
I have done this research on eggs for the welfare of mankind now i want to share my article for social awareness. I hope it will helps all researchers for their future further research.
Thank You
Ciprofloxacin resideu and their impact on Biomolecules n eggs of laying hens ...
Similar to Effect of Desloratadine on Oxidative and Inflammatory Ovarian Ischemia-Reperfusion Injury in Female Rats: Biochemical and Histopathological Evaluation
Protective effects of commelina benghalensis linn (root) extract on ethanol i...IJSIT Editor
The present study was undertaken to investigate the protective effect and possible mechanism of
alcoholic (AlE) and aqueous extract (AqE) from Commelina benghalensis root (CB) on EtOH-induced hepatic
injury in Wistar rat. Hepatotoxic parameters studied in vivo include serum transaminases (AST, and ALT),
ALP, bilirubin, protein, lipid profile (Cholesterol, triglyceride, VLDL and HDL) and level of antioxidants
together with histopathological examination. Liv 52® was used as a reference hepatoprotective agent
(5ml/kg-1b.w.). AlE and AqE (200 mg/kg-1b.w.) on oral administration decreased the level of AST, ALP, ALT,
bilirubin, cholesterol, triglyceride, VLDL, MDA and increased the level of protein, HDL and antioxidants (SOD,
GSH and CAT) in rats being treated with ethanol (EtOH). Pentobarbitone -induced sleeping time study was
carried out to verify the effect on microsomal enzymes Histopathological observations confirmed the
beneficial roles of MF against EtOH-induced liver injury in rats. Possible mechanism may involve their
antioxidant activity
Background: Body of literature are becoming pronounced that pathological condition in one organ of the body might have an effect on other distal organs owing to the fact, that the entire body metabolism is orchestrated centrally.
Pathological events occurring in an organ are likely to be extended to other organs. Pretreatment that minimize these events are presumed to be beneficial to the extended organs.
Methods: Following 30 min of ischemia and 48 h of reperfusion in the kidney, rats under anesthesia were sacrificed and blood sample collected through cardiac puncture. Serum level of troponin I, and activities of total creatine kinase (CK), mass creatine kinase (CK-MB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and gamma –glutamyl transferase (GGT) were estimated spectrophotometrically.
Results: Serum troponin I increased to 0.031 ± 0.001 ng/ml in the ischemic group, and following pretreatment with Lmm (600mg/kg), serum level of troponin I decreased significantly to 0.021 ± 0.001 ng/ml (P<.05).><.05),><.05)><.05).
Objective: Ischemia-reperfusion (I/R) leads to reactive oxygen species formation and cell death in kidney tissue with injury and organ transplantation. Simvastatin (SIM) is an antioxidant, anti-inflammatory, and anticoagulant agent. Alterations in I/R-induced acute kidney injury model with SIM treatment were analyzed.
Study Design: Wistar rats (n=28) were grouped into Sham, Ischemia, I/R, and I/R+SIM treated. Left rat kidney renal vessels were clamped for 60 minutes for ischemia, and the I/R group had 6 hours of reperfusion. 10 mg/kg SIM was given orally for 28 days. MDA, GSH, and MPO were analyzed. Kidney tissues were paraffin embedded, and primary antibodies TNF-α and caspase-3 were applied for immunohistochemistry.
Results: In the I/R group, intense inflammatory cell infiltration around the vessels and necrosis in the glomerular structures were observed. In the treated group, proximal and distal tubular cells were found to be close to normal. Immunoexpression of caspase-3 in the ischemia group was positive in degenerative glomeruli. In the treated group, TNF-α expression was negative in the glomerular structures. MDA and MPO levels were significantly increased in ischemia and I/R.
Conclusion: We suggest that SIM treatment improved kidney tissue structure and function in a model of I/R injury.
Keywords: caspase-3; immunohistochemistry; ischemia/reperfusion; kidney; MPO; simvastatin
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
A Study on the Toxic Effect of Different Doses of Diclofenac Sodium on the De...Prof. Hesham N. Mustafa
SUMMARY: The toxic effects of different doses of diclofenac sodium (DS) on the kidney on the postnatal period (0-7 days) by
morphometrical and immunohistochemical methods were investigated. For this purpose, 15 female adult wistar albino rats were used and
divided into 5 main groups. Group Ia served as normal control, physiologic group Ib received normal saline, group II received low dose (3.9
mg/kg), group III received medium dose (9 mg/kg) and group IV received high dose (18 mg/kg). Male offspring’s from 0-7 days after birth
were used in this study. On the 8th day of postnatal life, all animals were anesthetized. Then, the kidney samples were analyzed. Haematoxylin
and eosin staining showed degeneration and necrosis, apparent atrophy of the glomeruli, mononuclear cell infiltration, congested vessels,
increased fibrous tissue and distortion of the proximal convoluted tubules with interruption of the brush margin of the DS treated group.
Increased level of Caspase-3 and upregulation of TNF-α with different doses of DS. In light of our findings, DS may lead to adverse effects
that are dose-dependent in the prenatal subjected kidney to this drug.
KEY WORDS: Diclofenac sodium; Proximal convoluted tubules; Apoptosis;Cyclooxygenase.
A study on the toxic effect of different doses of Diclofenac sodium on the de...Prof. Hesham N. Mustafa
The toxic effects of different doses of diclofenac sodium (DS) on the kidney on the postnatal period (0-7 days) by morphometrical and immunohistochemical methods were investigated. For this purpose, 15 female adult wistar albino rats were used and divided into 5 main groups. Group Ia served as normal control, physiologic group Ib received normal saline, group II received low dose (3.9 mg/kg), group III received medium dose (9 mg/kg) and group IV received high dose (18 mg/kg). Male offspring’s from 0-7 days after birth were used in this study. On the 8th day of postnatal life, all animals were anesthetized. Then, the kidney samples were analyzed. Haematoxylin and eosin staining showed degeneration and necrosis, apparent atrophy of the glomeruli, mononuclear cell infiltration, congested vessels, increased fibrous tissue and distortion of the proximal convoluted tubules with interruption of the brush margin of the DS treated group. Increased level of Caspase-3 and upregulation of TNF-α with different doses of DS. In light of our findings, DS may lead to adverse effects that are dose-dependent in the prenatal subjected kidney to this drug.
Keywords : Diclofenac sodium; Proximal convoluted tubules; Apoptosis; Cyclooxygenase.
STUDY ON ANTI ULCER AND ANTI INFLAMMATORY EFFECTS OF Vilvathi LehiyamJing Zang
The aim of the study, to evaluate the phytochemical, anti-ulcer and anti-inflammatory activities of Vilvathi Lehiyam. Anti-ulcer activity of ethanolic extract of Vilvathi Lehiyam was investigated on omeprazole induced ulcer model in albino rats. Ethanolic extract of dosage 250 and 500kg/mg produced significant inhibition of gastric lesions induced by Omeprazole induced ulcer. The extract 250 and 500kg/mg showed significant (p<0.01) reduction of pH value of gastric juice compared control. The Vilvathi Lehiyam was evaluated for anti-inflammatory activity against the carrageenan induced rat paw oedema at injected sthe doses 500 kg/mg body weight and the study was compared with standard drug Dexamethasone (2mg/kg). The Vilvathi Lehiyam has significant anti-inflammatory activity, which support the traditional medicinal utilization of Vilvathi Lehiyam. Based on the above results, of Vilvathi Lehiyam may be useful as a natural drug for the treatment of ulcer and inflammation.
Genotoxicity of Goji Berry (Lyciumbarbarum) In Vivo Mammalian Cellsinventionjournals
Lyciumbarbarum (Gojji berry) belongs to family Salonaceae which is found in China and Himalayan. This herb is used to prevent various diseases and in medical treatments as an alternative medicine being widely used for its antioxidant and revitalizing potential effects. In recent years, Gojji has become increasingly popular in Europe and North America as a "superfruit" and dietary supplement. The belief that herbal products do not bring any risk to health, is part of popular culture. However the term "natural" assigned to many products cannot assure no health risk. The aim of this study was to evaluate the possible genotoxic effects of aqueous extract of Lyciumbarbarum (Gojji berry) by micronucleus test and comet assay. Thirty Rattus norvegicus were divided into three equal groups: 1) experimental group, submitted to Gojji berry (200mg/kg orally); 2) positive control group (cyclophosphamide), and; 3) negative control group (distilled water). Micronucleus Tests were done by smear method of bone marrow cells performed after 48h for acute, and 72h for chronic exposure. The comet assay was performed on peripheral blood taken from the tail of each animal 4h, and 24h after intervention. Cytotoxicity was assessed by observing the DNA damage measuring the percentage of DNA in the tail (% DNA- measurement of the proportion of the total DNA present in the tail) and the tail moment (TM-tail length times the percentage of DNA in the tail), calculated by 100 nucleoids per animal and the presence of micronuclei in 2,000 polychromatic erythrocytes per animal. Analysis of variance (ANOVA) followed by Tukey test at 5% significance was used comparing the results. The data showed no significant difference in the frequency of DNA damage and the number of micronuclei between the experimental group and the negative control group. The results also suggest that the aqueous extract of Lyciumbarbarum (Gojji berry) at the dose of 200 mg/kg showed no genotoxic effect, which could, to a certain point, justifies its use.
Similar to Effect of Desloratadine on Oxidative and Inflammatory Ovarian Ischemia-Reperfusion Injury in Female Rats: Biochemical and Histopathological Evaluation (20)
BACKGROUND: Sequential Epstein-Barr virus (EBV)–positive B cell lymphoma to the initial diagnosis of angioimmunoblastic T cell lymphoma (AITL) is very rare, the exact mechanism and standard therapy of which is still being explored. CASE: A 50-year-old man was admitted to our hospital in January 2014 with a three-week history of enlargement of multiple lymph nodes. His initial pathological evaluation indicated AILT. The reactivation of EBV was observed during the immunosuppression therapy for AITL, accompanied by onset of subcutaneous nodules proven to be EBV-positive diffuse large B cell lymphoma (DLBCL) based on the pathological findings of rebiopsy. The patient was successfully treated with chidamide, a histone deacetylase (HDAC) inhibitor, and rituximab.
Conclusion: The sufficient surveillance for serum EBV and repeat biopsy is necessary for patients with AITL, and this treatment modality may become an active option.
Keywords: angioimmunoblastic T cell lymphoma, Epstein-Barr virus, HDAC inhibitor, non-Hodgkin lymphoma, peripheral T cell lymphoma
Objective: The association between telomerase reverse transcriptase (TERT) promoter mutation and outcome of melanoma is unclear and controversial. We aim to conduct a meta-analysis and investigate whether the TERT promoter mutation is a prognostic factor of melanoma.
Study Design: Appropriate studies were searched in 3 databases: PubMed, Web of Science, and Embase. Pooled hazard ratios (HRs) were counted through random effects model.
Results: Heterogeneity was moderate in overall survival (OS) (I2=43.7%, p=0.059) and low in disease-free survival (DFS) (I2=0.0%, p=0.587). Sensitivity analysis indicated that the removal of any of the study did not affect the final results. Evidence for publication bias was not found (Begg’s test, p=0.281; Egger’s test, p=0.078). The pooled OS HRs from combined effects analysis was determined (HR 1.07; 95% CI 0.83–1.39, p=0.585), together with the pooled HRs of DFS (HR 1.65; 95% CI 1.02–2.66, p=0.042). TERT promoter mutation predicted a good outcome in meta-static melanoma patients (HR 0.66; 95% CI 0.46–0.96, p=0.042). The pooled HRs of combined mutation in TERT promoter and BRAF (HR 6.27; 95% CI 2.7–14.58, p=0.000) predicted a bad outcome in melanoma patients.
Conclusion: TERT promoter mutation significantly predicted poor DFS outcome but, on the contrary, predicted a good outcome in metastatic melanoma patients. The combined TERT promoter and BRAF mutation was a significant independent factor of OS in melanoma patients.
Keywords: melanoma; meta-analysis; mutation; prognosis; promoter regions, genetic; skin neoplasms; telomerase; TERT promoter mutation; TERT protein, human
Objective: In order to reduce complications accompanied with dental implant restoration, this study strives to prepare a novel sealant and lubricant that can be used in dental implant systems as well as to evaluate its characteristics.
Study Design: Chitosan (CS), β-glycerophosphate pentahydrate (β-GP), and nano silver (nAg) were used to prepare thermosensitive hydrogel. According to the different volume ratios of CS to β-GP, 3 experimental groups were established, namely 16/4, 13/7, and 10/10 groups. Their morphology, composition, and chemical properties were analyzed via SEM, EDS, and FTIR. In addition, the effect of the hydrogel on the stability of dental implant-abutment connection was investigated by removal torque test combined with dynamic cyclic loading experiment. The maximum fracture load was measured under different lubricating conditions by electronic universal testing machine. The cytotoxicity and in vitro antibacterial effect of the hydrogel were examined respectively by CCK-8 test and the spread plate method.
Results: The CS/β-GP/nAg thermosensitive hydro-gel was successfully prepared in this study, which was found to be a porous structure through SEM. The removal torque test and the dynamic cyclic loading experiment showed that the removal torque of the experimental group was greater than that of the control group. Furthermore, the single load-to-fracture test indicated that the 16/4 group had the greatest maximum bearing load. The in vitro cytotoxicity test using rat bone marrow stromal cells (rBMSCs) and human gingival fibroblast cells (hGFCs) showed no cytotoxicity in all 3 groups. The 3 experimental groups had obvious antibacterial effects against E. coli, S. aureus, and P. gingivalis.
Conclusion: A nontoxic antibacterial CS/β-GP/nAg thermosensitive hydrogel for lubricating purpose was successfully fabricated. When the volume ratio of CS to β-GP was 16/4, this thermosensitive hydrogel demonstrated better sealing and lubricating abilities and had a positive influence on the reliability of dental implant-abutment connection.
Keywords: abutment, dental implant, dental implant restoration, dental sealant, lubrication, thermosensitive hydrogel
Objective: To investigate the bond strength of resin-modified glass ionomer enhanced with bioactive glass (Activa BioActive-Base/Liner) to composite resin using different dental adhesive systems.
Study Design: In this study, Activa BioActive-Base/Liner (ABA/BL) was placed in cylindrical cavities formed in acrylic blocks. In blocks divided into 6 groups according to the adhesive system to be applied, two-step etch-and-rinse Gluma 2 Bond (Heraeus Kulzer, Germany), one-step self-etch Gluma Self Etch (Heraeus Kulzer), universal system Gluma Universal (Heraeus Kulzer), two-step self-etch Clearfil SE Protect (Kuraray, Japan), one-step self-etch Clearfil S3 Bond Plus (Kuraray), and universal system Clearfil S3 Bond Universal (Kuraray) adhesive systems were applied on ABA/BL. After composite resin (3M ESPE Filtek Ultimate) was applied to the prepared surfaces, the specimens were placed in a universal test device and shear bond strength test was determined. Fracture types were evaluated using a stereomicroscope and scanning electron microscope. Data were analyzed by Shapiro-Wilk, two-way ANOVA, Kruskal-Wallis, and Post-Hoc Multiple Comparisons tests.
Results: In terms of bond strength values, the highest bond value was seen in the two-step self-etch (Clearfil SE Protect) group, and the lowest bond strength value was seen in the universal system (Clearfil S3 Bond Universal) group. There was no statistically significant difference between the adhesive agent groups in terms of bond strength values (p>0.05).
Conclusion: It is thought that choosing the two-step self-etch technique as an adhesive system when resin-modified glass ionomer enhanced with bioactive glass (ABA/BL) is used as the pulp capping/base material will be more appropriate in terms of bond strength.
Keywords: adhesive systems, bioactive materials, bond strength, cariostatic agents, composite resins, dental materials, fluorides, glass ionomer, glass ionomer cements, materials testing, vital pulp therapy
Objective: To analyze the sonographic features of different histopathological subtypes of borderline ovarian tumors (BOTs) confirmed by pathology, and to study the ultrasound performances of various types in borderline ovarian tumors.
Study Design: Retrospective analysis was performed on the pathological results and ultrasound projection findings of 129 patients diagnosed as BOTs by ultrasound department of our hospital from January 2012 to November 2019. All patients were confirmed by surgical pathology and scanned consecutively by the investigators using transabdominal or transvaginal ultrasound examination.
Results: Serous borderline tumors (SBOTs) were observed, and the prevalence rate (53%) was significantly higher than that of other subtypes, and the probability of bilateral lesions was higher (40%). The sonogram often showed ultrasound features of papillary neoplasm in the lesion and good internal echo (p<0.05). Mucinous borderline ovarian tumors (MBOTs) were mostly unilateral lesions (86%). The prevalence was second only to SBOTs. Histomorphological examinations were divided into gastrointestinal-type and endocervical-type. Among them, the gastrointestinal type of MBOTs were mostly unilateral, and their incidence was higher than that of endocervical-type of MBOTs. Compared with other pathological subtypes, the gastrointestinal type is more likely to show the sonographic characteristics of huge space occupying in the pelvic and abdominal cavity (mean diameter >10 cm), polycystic, multiple septums, and poor internal echo (p<0.05). The ultrasonographic features of the endocervical-type of MBOTs were similar to those of SBOTs. Compared with gastrointestinal type, the sonographic images showed smaller lesion diameter, less septal or cyst, and more papillary excrescences in the tumor (p<0.05). The borderline clear cell tumor is the intermediate transition between the clear cell adenofibroma and the clear cell carcinoma. The clinical manifestations are diverse and lack specificity. The histology of sonography was mainly solid, and the multiple microcapsules were honeycomb-like. It can also be shown as cystic. Among the 169 patients with BOTs, 20 cases of SBOTs, 17 cases of MBOTs, and 10 cases of other rare subtypes were complicated with other diseases or multiple subtypes. This study did not find significant ultrasonic characteristics were used for distinguish them from other subtypes.
Conclusion: BOTs is a common disease in women during the reproductive period. It is characterized by the development of malignant tumors. Its clinical and pathological subtypes are complex and diverse. It leads many doctors to use the terms “large pelvic mass” and “solid ovarian mass” for diagnosis because of their lack of experience and understanding.
Keywords: adenocarcinoma, mucinous; adenocarcinoma, serous; borderline ovarian tumors; diagnostic imaging; ovarian neoplasms; papillary neoplasms; prognosis; transvaginal ultrasound, ultrasonography
Objective: The prognostic indictors of age-related poor outcomes in patients with acute myeloid leukemia (AML) are still controversial. The aim of this work was to provide comprehensive insights into the effect of different hemocytes and to investigate the association between age and clinical features in adult patients with AML.
Study Design: A retrospective study was performed to determine the role of age in the therapeutic outcomes of AML. A total of 166 newly diagnosed adult patients’ data from January 2015 to November 2019 in Zhongshan Hospital of Xiamen University were collected and analyzed.
Results: Older patients presented a poorer prognosis (p=0.001) with shorter overall survival, which is served as age-related outcomes. Binary logistic regression demonstrated that cytogenetic risk (OR=4.508, 95% CI 2.733–7.435), leukocyte (OR=7.410, 95% CI 1.139–5.910), and bone marrow blast cells (OR=3.261, 95% CI 1.075–5.615) were independent indictors for age-related prognosis. In addition, Kaplan-Meier curve also revealed that the above factors were associated with overall survival (all p values <0.001).
Conclusion: Cytogenetic risk, leukocyte, and bone marrow blast cells are dominant factors which account for the age-related poor outcomes and shorter overall survival in AML.
Keywords: acute myeloid leukemia, adult, cytogenetic risk, hemocyte, leukemia, overall survival
Objective: To investigate the effects of nicorandil and tirofiban on no-reflow and postoperative outcome in patients with acute coronary syndrome (ACS) undergoing percutaneous coronary intervention.
Study Design: A total of 438 patients with ACS diagnosed by the second Hospital of Shanxi Medical University from January 2019 to December 2020 were divided into two groups: nicorandil group (n=223) and tirofiban group (n=215). The nicorandil group was injected with 2 mg nicorandil 2 mm before coronary occlusion before balloon dilation, and the tirofiban group received 10 μg/kg intravenous injection during operation. Measurement of thrombolysis grade (thrombolysis in myocardial infarction [TIMI]), corrected TIMI frame count, and major adverse vascular events were recorded 30 days after operation in patients with ACS.
Results: Both nicorandil and tirofiban could improve the TIMI grade, and TIMI grade 3 blood flow was obtained in 190 cases (85.2%) and 175 cases (81.4%), respectively. There was no significant difference in the incidence of major adverse cardiac events (14.3% vs. 13.5%, score 0.13).
Conclusion: Intracoronary use of nicorandil in patients with ACS can improve coronary perfusion, but the improvement of prognosis needs further study.
Keywords: coronary perfusion, myocardial infarction, nicorandil, no-reflow phenomenon, percutaneous coronary intervention, repercussion
Objective: To identify interstitial cells of Cajal (ICC) in the common bile duct of Kunming mice.
Study Design: Common bile ducts obtained from the Kunming mice were prepared for immunohistochemical investigations using the c-kit antibody. Immunoelectron microscopy was used to detect the expression of c-kit in the ICC of the common bile duct. Transmission electron microscopy showed ultrastructure of ICC in the murine bile duct. Reverse transcription–polymerase chain reaction (RT-PCR) and western blot were used to confirm the expression of mRNA specific for the c-kit gene and production of c-kit protein in the Kunming mice common bile duct.
Results: Immunohistochemistry revealed that ICC in the murine common bile duct are c-kit positive and the ICC are located in the tela submucosa and the tunica muscularis of the murine common bile duct and do not connect with each other. Immunoelectron microscopy confirmed the expression of Kit by ICC in the murine common bile duct. Transmission electron microscopy showed that ICC in the murine common bile duct have long processes, abundant mitochondria, plenty of smooth endoplasmic reticulum (sER), a lot of lysosomes, and dense bodies. The caveolae of ICC are distinctive. At the same time, RT-PCR indicated that the Kunming mice common bile duct expressed mRNA specific for the c-kit gene, and western blot analysis showed the evidence of production of c-kit protein in the Kunming mice common bile duct.
Conclusion: ICC are found in the Kunming mice common bile duct, which is likely to lead to the development of motility study of the common bile duct.
Keywords: common bile duct; electron microscopy; immuno-electron microscopy; interstitial cells of Cajal; intestines; smooth muscle; tyrosine kinase receptor (c-kit)
Objective: To evaluate the antibacterial effects of 4 different cavity disinfectants on Streptococcus mutans, Lactobacillus acidophilus, and Enterococcus faecalis bacteria in different time periods.
Study Design: The antibacterial effects of Cavity Cleanser, Tubulicid Red Label, Chloraxid 2%, and Oxygenated Water cavity disinfectant solutions on E. faecalis (ATCC 29212), S. mutans (ATCC 25175), and L. acidophilus (RSKK 03037) bacterial strains were evaluated by disk diffusion method. In the study where vancomycin antibiogram disc constituted the positive control group, physiological saline solution was used as the negative control group. Standard, sterile, blank antibiogram discs of 5 mm in diameter, in which 15 μL of each material were added, were placed on agar plates at 2.5–3 cm intervals. The inhibition zone diameters formed around the discs that were left to incubate for 24–48 hours at 37°C were measured in millimeters. Statistical analysis of the data was performed using one-way analysis of variance, Kolmogorov-Smirnov, Levene, and Bonferroni tests.
Results: At the end of the study the solutions tested showed a statistically significant antibacterial effect on all bacterial strains used (p<0.05). Cavity Cleanser disinfectant containing 2% chlorhexidine showed the highest antibacterial effect on S. mutans and L. acidophilus, and benzalkonium-containing Tubulicid Red disinfectant on E. faecalis.
Conclusion: The antibacterial effect of all cavity disinfectants used in the study was found to be higher at the end of the 48th hour than at the end of the 24th hour, but there was no statistically significant difference (p>0.05).
Keywords: antibacterial agents; antibacterial effect; cavity disinfectants; chlorhexidine; contamination; dental caries; disinfection; disc diffusion; gram-negative bacteria; gram-positive bacteria
Objective: To probe into the influence of miR-21 on the proliferation as well as apoptosis of oral squamous cell carcinoma (OSCC) and its causative role.
Study Design: We adopted microarray for detecting the differentially expressed genes in OSCC tumor tis-sues and paracancerous tissues. We assessed the link of miR-21 expression with tumor size, lymph node metastasis, and tumor differentiation. We employed CCK-8 and EdU assay for detecting the impact of miR-21 inhibitor and miR-21 mimic on Cal-27 cell proliferation, as well as TUNEL and AnnexinV-FITC/PI double staining for detecting miR-21 expression on cell apoptosis. We forecasted the possible target of miR-21 via TargetScan, as well as detected the interaction of miR-21 with PTEN via luciferase reporter experiment. The function of miR-21 expression in PTEN signaling pathway was monitored via western blot. We constructed PTEN overexpression plasmid and conducted rescue experiment to evaluate overexpressed PTEN on miR-21–induced proliferation.
Results: Microarray and RT-qPCR indicated that miR-21 expression increased demonstrably in OSCC. Subsequently, statistical analysis showed that miR-21 expression was plainly correlated with tumor size, lymph node metastasis, tumor differentiation, and smoking history. CCK-8 and EdU method exhibited that miR-21 mimics manifestly promoted Cal-27 cell proliferation, while miR-21 inhibitor blatantly inhibited Cal-27 cell proliferation. TUNEL and V-FITC/PI double staining assay showed that miR-21 inhibitor conspicuously promoted Cal-27 cell apoptosis. CCK-8 and EdU assay exhibited that overexpressed PTEN abolished the pro-proliferation influence of miR-21 mimic. TUNEL and V-FITC/PI experiments pointed out that knocking down PTEN abrogated the pro-apoptosis impact of miR-21 inhibitor.
Conclusion: miR-21 contributes to OSCC cell proliferation via targeting PTEN and inhibits its apoptosis.
Keywords: Akt/PKB signaling pathway; apoptosis; biomarkers, tumor; carcinoma, squamous cell; cell line, tumor; cell proliferation; microRNAs; miR-21; miRNA-21; mouth neoplasms; oral cancer; oral squamous cell carcinoma; proliferation; real time PCR
Objective: To investigate the changes in the retina due to deltamethrin toxicity and the process in cell inflammation and apoptosis.
Study Design: Sixteen Wistar albino rats were randomly divided into two groups as control (n=8) and deltamethrin (n=8) groups. Saline was given to the control group, and 0.5 mL of 5 mg/kg deltamethrin was given to the deltamethrin group for 14 days each. Blood was collected for biochemical analysis. Retinal tissue was processed for histological examination.
Results: Compared to the control group, MDA levels were high while GSH and CAT levels were low in the deltamethrin group. Histopathological analysis showed spaces between the pigment epithelium, irregularity in the delimiting membrane, degenerated ganglion, cone and bacillus cell, pyknotic nuclei, thinned inner limitation membrane, and thickened vascular wall. The control group showed FAS expression in the pigment layer limiting membranes, in the nuclei of many cone and bacillus cells, and ganglion cells in the control group sections. In the deltamethrin group, FAS expression was observed in the inner and outer limiting membranes of the pigment epithelium, cone and bacillus cells, and ganglion cell nuclei. In the control group, negative NOS expression in the pigment epithelium and outer limiting membranes, internal limitation membrane, and ganglion cells in the cone and bacillus cell nuclei were observed. In the deltamethrin group, NOS expression was positive in the pigment epithelium, cone and bacillus, and ganglion cell nuclei.
Conclusion: We suggest that deltamethrin toxicity induced apoptotic process due to increased inflammation in the retina and may cause visual impairment as a result of neural damage.
Keywords: deltamethrin, FAS, insecticides, NOS, nitric oxide synthase, retina
Objective: Tongue squamous cell carcinoma (TSCC) is a prominent type of oral cancer. Despite the numerous research studies on SCC and microRNAs (miRs), the relation between TSCC and miR-135b-5p is poorly discussed. This experiment aims to find out the possible effect of miR-135b-5p on TSCC with the network of its downstream genes.
Study Design: TSCC tissues and adjacent normal tissues were harvested. Then, expression of miR-135b-5p and AT-rich interactive domain‑containing protein 1A gene (ARID1A) and the phosphatidyl inositol 3-kinase/protein kinase B (PI3K/AKT) pathway was analyzed. After the transfection of miR-135b-5p inhibitor and its negative control into TSCC cells, functional assays were employed to measure cell proliferation, apoptosis, and cycle. Next, the target relation between miR-135b-5p and ARID1A was confirmed. In addition, the fact that miR-135b-5p promoted TSCC development via mediating ARID1A was demonstrated by functional rescue experiment.
Results: miR-135b-5p was upregulated in TSCC tissues and cells, while ARID1A was suppressed (p< 0.05). Silenced miR-135b-5p discouraged TSCC cell proliferation, improved apoptosis, induced cell cycle arrest, and increased ARID1A expression while inactivating the PI3K/AKT axis (p<0.05). Furthermore, knockdown of ARID1A reversed the impacts on TSCC cell proliferation and apoptosis exerted by silencing miR-135b-5p.
Conclusion: This research supported that silenced miR-135b-5p impeded TSCC proliferation and apoptosis by promoting ARID1A and inactivating the PI3K/AKT axis, which may provide some indications for TSCC alleviation.
Keywords: apoptosis; ARID1A; ARID1A protein, human; carcinoma, squamous cell; cell line, tumor; cell proliferation; drug resistance, neoplasm; microRNA-135b-5p; microRNAs; PI3K/AKT pathway; neoplasm metastasis; neoplastic stem cells; proliferation; protein binding; tongue; tongue squamous cell carcinoma
Objective: To examine the oropharynx of patients with ectodermal dysplasia showing maxillary retrusion and mandibular protrusion with a short and concave facial structure using cone-beam computed tomography method. Ectodermal dysplasia refers to the congenital disorder defined by the abnormal development of the structure originating from the ectoderm.
Study Design: In order to examine the oropharynx airway, measurements and statistical evaluations were made in 3 levels in sagittal and transversal directions on three-dimensional cone beam computed tomography images obtained from 14 individuals divided into 2 groups as Ectodermal Dysplasia group (n=7) and Control group (n=7).
Results: As a result of statistical analysis, no statistically significant difference was found between the groups at any level or direction in metric measurements performed on all 3 planes taken at the sagittal and transversal levels (p>0.05).
Conclusion: Our findings on ectodermal dysplasia are similar to Class III malpositions that show similarity with ectodermal dysplasia.
Objective: Diabetic nephropathy is one of the most serious complications of diabetes mellitus. It develops in approximately one-third of diabetic patients, years after the onset of metabolic abnormalities.
Study Design: The biopsy specimens were evaluated with the focus on light microscopy. The aim of our study was to reveal differences in the details and the frequency of occurrence of individual histomorphological changes in diabetic nephropathy and other glomerulonephritides.
Results: Diabetic nephropathy accounted for 14 out of 82 analyzed biopsies. Isolated thickening of the glomerular basement membrane was not present in any case, but along with some degree of mesangial expansion, hypercellularity or glomerulosclerosis was seen in 12 out of 14 findings of diabetic nephropathy. In other glomerular diseases, mesangial changes, but without glomerular basement membrane thickening, were the most frequent findings. In addition to glomerular lesions, some of the tubular, interstitial, and vascular changes were seen in 13 out of 14 patients with diabetic nephropathy. In other glomerulonephritides the combination of all these changes was a rare finding.
Conclusion: There are cases where immunofluorescence and electron microscopy cannot be performed or their results are not helpful. In such cases we must rely on light microscopic histomorphological changes.
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
Anti ulcer drugs and their Advance pharmacology ||
Anti-ulcer drugs are medications used to prevent and treat ulcers in the stomach and upper part of the small intestine (duodenal ulcers). These ulcers are often caused by an imbalance between stomach acid and the mucosal lining, which protects the stomach lining.
||Scope: Overview of various classes of anti-ulcer drugs, their mechanisms of action, indications, side effects, and clinical considerations.
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
2. ular oxygen (O2) supplied with the blood reacts
with xanthine oxidase (XO), which accumulates
in ischemic tissue and initiates the conversion
of hypoxanthine to xanthine in reperfusion. It
causes an increase in the production of reactive
oxygen species (ROS) and a decrease in antioxi-
dant defense mechanisms.3 The elevation in ROS
increases the production of nuclear factor–κB
(NF-κB), a proinflammatory cytokine.4 NF-κBs
have been shown to induce the secretion of pro
inflammatory interleukin 1 beta (IL-1b), tumor ne-
crosis factor alpha (TNF-a), and other inflamma
tion mediators.5 As is known, in addition to oxi-
dants, proinflammatory cytokines such as IL-1b,
TNF-a, and NF-kB have also been reported to
play a significant role in the pathogenesis of
ischemia-reperfusion (I/R) injury.6,7 Ovarian I/R
injury occurs in the clinic after the detorsion pro
cedure is performed in order to ensure reperfu-
sion in torsioned ovaries. Ovarian torsion, which
is the most common gynecological emergency,
has a prevalence of 2.7%.8 Delays in the diagnosis
and treatment of ovarian torsion have been re-
ported to result in severe ovarian damage and in
fertility.9 In clinical practice the treatment strategy
of torsioned ovaries is to preserve ovarian func-
tion with detorsion as soon as possible rather than
ovariectomy. However, as stated above, reper-
fusion of torsion-induced ischemia by detorsion
leads to exacerbation of oxidative and inflam-
matory events in the ovarian tissue. Therefore,
studies on the pathogenesis of I/R injury and its
treatment are still undertaken in the attempt to
minimize torsion-detorsion damage. Deslorata-
dine, which will be examined in this study for
its protective effect against ovarian I/R injury,
is a potent, non-sedating H1 histamine receptor
antagonist indicated for symptomatic relief of
urticaria and allergic rhinitis.10 Roumestan et al
have shown that desloratadine inhibits NF-κB pro-
duction.11 Moreover, Wu et al have argued that
desloratadine inhibits both basal and histamine-
induced NF-κB.12 In subsequent studies, it has
been reported that desloratadine antagonizes the
increase of malondialdehyde (MDA), the produc-
tion of lipid peroxidation, and the decrease of
endogenous antioxidant glutathione (GSH) in the
allergy model.13 This information suggests that
desloratadine can protect ovarian tissue from I/R
injury. There is no study in the literature in-
vestigating the protective effect of desloratadine
against ovarian I/R injury. In addition, there are
no studies in the literature investigating the ef-
fects of desloratadine on proinflammatory cyto-
kines such as IL-1β, TNF-a, and NF-kB, which play
a role in the pathogenesis of I/R-related ovarian
damage. Again, it has been observed that the pro-
tective effect of desloratadine against oxidative
stress due to MDA increase and tGSH decrease
in ovarian tissue during I/R has not been inves-
tigated. Therefore, the aim of this study is to in-
vestigate the effect of desloratadine on proinflam
matory cytokines such as IL-1β, TNF-a, and NF-kB
and oxidant/antioxidant parameters on ovarian
I/R injury and to examine the histopathological
effect on ovarian tissue.
Materials and Methods
Animals
Thirty female albino Wistar rats (6 months old)
weighing between 265–278 g were used in this
study. The animals were obtained from Ataturk
University Medical Experimental Application and
Research Center. The animals were kept (7 days)
in the laboratory of the Department of Pharmacol-
ogy at normal room temperature (22°C) and were
fed with standard feed to adapt them to the
study environment. During this time, the rats had
24-hour/day access to normal animal feed and tap
water. The female rats that were to be used in the
experiment were separated from male rats shortly
after they were weaned. The protocols and proce-
dures were approved by the local Animal Experi-
mentation Ethics Committee (date June 27, 2019,
meeting no. 7).
Chemicals and Experimental Design
The Ketamine used in the experiment was ob-
tained from Pfizer Inc. (Turkey), and the deslo-
ratadine was obtained from Sanofi Ilac ve Sanayi
Ticaret–Turkey. Female rats were divided into three
groups: (1) OIR group (rats with induced ovarian
I/R), (2) OIRD group (rats with induced ovarian
I/R and treated with 15 mg/kg desloratadine, and
(3) sham group.
Surgical Procedures
Desloratadine (5 mg/kg) was administered via
oral gavage in the OIRD group before I/R was
induced in rat ovaries. Different commercial
forms and doses of desloratadine have been used
in previous experimental studies.14,15 OIR and
sham group rats were treated with an equal vol-
ume of distilled water used as solvent. After ad-
62 Analytical and Quantitative Cytopathology and Histopathology®
Kadıoglu et al
3. ministration of desloratadine and distilled water,
all rats were anesthetized with 60 mg/kg IP keta
mine. During this period, the lower part of the
abdomen was opened vertically at a length of
2–2.5 cm, and the ovaries were reached. Then,
vascular clips were applied to the lower part of
the right ovary (the region where the ovary was
connected to the uterus) of the OIRD and OIR
rats for 2 hours to induce ischemia. This proce-
dure was followed by 2 hours of reperfusion.16
The reason for choosing the right ovary in our
study was that adnexial torsion is more common
on the right side (66%) than on the left side in
clinic.17 The reason for our application of 2 hours
of ischemia and 2 hours of reperfusion was that
oxidant and proinflammatory parameters were
significantly increased in the ovarian tissue dur-
ing this period, and it has been observed that
serious oxidative and inflammatory damage de
veloped in the ovarian tissue.18 The ovaries of
the sham group were closed without ischemia.
After reperfusion, all animals were sacrificed with
a high dose of anesthesia. The right ovaries of
the sacrificed rats were removed, and biochemi-
cal and histopathological examinations were per-
formed in the ovarian tissue. The results obtained
from the OIRD and sham groups were compared
with those obtained from the OIR group.
Biochemical Operations
MDA Assessment. The method used by Ohkawa
et al was taken as the basis for MDA assessment.19
This method is based on the spectrophotometric
assessment of the absorption of the pink complex
created by thiobarbituric acid (TBA) and MDA at
high temperature (95°C) at 532 nm wavelength.
Homogenates were centrifuged for 20 minutes at
5,000 g, and these supernatants were used in the
determination of the amount of MDA. 250 μL
homogenates, 100 μL 8% sodium dodecyl sulfate
(SDS), 750 μL 20% acetic acid, 750 μL 0.08% TBA,
and 150 μL distilled water were vortexed into
capped test tubes through a pipette. The mixture
was incubated for 60 minutes at 100°C. 2.5 mL
n-butanol was added to the mixture, and then
spectrophotometric analysis was performed. The
amounts of resultant red color were measured
using 3 mL cuvettes at 532 nm. MDA amounts of
the samples were determined by using the stan
dard graphics formed with the MDA stock solu-
tion, which was previously prepared considering
the dilution coefficients.
tGSH Assessment. The DTNB [5,5’-Dithiobis (2-
nitrobenzoic acid)] in the assessment environment
is a disulfide chromogen and is easily reduced by
sulfhydryl group compounds. The resultant yel-
low color was spectrophotometrically assessed at
412 nm.20 Homogenates were centrifuged for 10
minutes at 12,000 g, and the supernatants were
used in the determination of the amount of MDA.
250 μL measuring buffer (200 mM Tris-HCl,
pH=8.2 involving 0.2 mM EDTA), 500 μL super-
natant, 100 μL 5,5’-Dithio-bis (2-nitrobenzoic acid)
(DTNB), and 7,900 μL methanol were vortexed into
capped test tubes through a pipette. The mix-
ture was incubated for 30 minutes at 37°C, and
then spectrophotometric analysis was conducted.
The amounts of resultant yellow color were mea-
sured using 3 mL quartz cuvettes at 412 nm. GSH
amounts of the samples were determined by using
the standard graphics formed with the GSH stock
solution, which was previously prepared consider-
ing the dilution coefficients.
NF-κB, TNF-a, and IL-1b Analysis. Tissue homoge-
nate NF-κB and TNF-a concentrations were mea-
sured using rat-specific sandwich enzyme-linked
immunosorbent assay. Rat NF-κB ELISA immu
noassay kits (Cat. No. 201-11-0288, SunRed) and
Rat TNF-a and Rat IL-1b ELISA kits (Cat No.
YHB1098Ra, Shanghai LZ) were used. Analyses
were performed according to the manufacturers’
instructions. Briefly, monoclonal antibody specific
for rat NF-κB, TNF-a, and IL-1b were coated onto
the wells of the micro plates. The tissue homoge-
nate, standards and biotinylated monoclonal anti-
body specific and streptavidin-HRP were pipetted
into these wells and then incubated at 37°C for 60
minutes. After washing, chromogen reagent A and
chromogen reagent B were added, which is acted
upon by the bound enzyme to produce a color. It
was incubated at 37°C for 10 minutes. Then stop
solution was added. The intensity of this colored
product is directly proportional to the concentra-
tion of rat NF-κB, TNF-a, and IL-1b present in the
original specimen. At the end of the course, the
well plates were read at 450 nm. The absorbance
of the samples was calculated with formulas that
used standard graphics.
Histopathological Examination
All of the tissue samples were first identified in a
10% formaldehyde solution for light microscope
assessment. Following the identification process,
Volume 42, Number 2/April 2020 63
Desloratadine for Ovarian Ischemia-Reperfusion Injury
4. tissue samples were washed under tap water in
cassettes for 24 hours. Samples were then treated
with conventional grade of alcohol (70%, 80%, 90%,
and 100%) to remove the water within tissues. Tis-
sues were then passed through xylol and embed-
ded in paraffin. Four-to-five micron sections were
cut from the paraffin blocks, and hematoxylin-
eosin staining was administered. Their photos were
taken following the Olympus DP2-SAL firmware
program (Olympus Inc., Tokyo, Japan) assessment.
Histopathological assessment was carried out by
the pathologist blind for the study groups.
Results
Biochemical Findings
MDA and tGSH Analysis Results. As can be seen in
Figure 1, the amount of MDA in ovarian tissue of
the ovarian I/R group was 16.6±1 μmol/g protein,
while the amount of tGSH was 7.6±0.2 nmol/g
protein. In the group treated with desloratadine,
the amount of MDA is 7.6±0.2 μmol/g protein,
while the amount of tGSH is 21±1.1 nmol/g pro
tein. The MDA value of the sham group was
5.8±0.3 μmol/g protein; tGSH was recorded as
26.5±0.8 nmol/g protein. MDA production signi-
ficantly increased in the kidney tissue of animals
treated with I/R as compared to the sham group
and the desloratadine-treated OIRD group (p<
0.0001). The difference in MDA levels between the
desloratadine and sham groups was statistically
insignificant (p>0.05). In addition, the I/R proce
dure caused a decrease in tGSH in the kidney
tissue. tGSH levels in the sham and desloratadine
groups were significantly higher as compared to
the I/R group (p<0.001) (Figure 1).
NF-κB, IL-1b, and TNF-a Analysis Results. The mea-
surements of NF-κB in ovarian tissue of the ovar-
ian I/R group was 8.8±0.2, of the desloratadine-
treated group was 2±1.8, and of the sham group
was 1.6±1.1 ng/mL. The amounts of IL-1b were
11.6±0.7, 3.5±2, and 2.8±0.2 pg/mL. TNF-a lev-
els were 9.2±0.2, 3.3±0.2, and 2.4±0.1 pg/mL,
respectively. I/R significantly increased NF-κB,
IL-1b, and TNF-a levels in ovarian tissue as
compared to in the sham and desloratadine-
treated groups (p<0.0001). The difference in NF-
κB, IL-1b, and TNF-a levels between the sham
and desloratadine-treated groups was statistically
insignificant (p>0.05) (Figure 2).
Histopathological Findings
In Figure 3, no pathological findings were found
in ovarian tissue of the sham group that under-
went sham operation. In addition, ovarian tissue
consisted of developing follicle structures and
corpus luteum. However, diffuse congestion and
hemorrhage were observed in ovarian tissue in
the OIR group treated with I/R procedure. Spo-
radic fluid accumulation and inflammatory cell
clusters/infiltrates were also observed in the
subcapsular area, and necrotic changes were ob-
served in the parenchyma, stroma, and follicle
structures (Figure 4). In the OIRD group treated
with desloratadine, congestion in ovarian tissue
decreased as compared to in the OIR group. How-
64 Analytical and Quantitative Cytopathology and Histopathology®
Kadıoglu et al
Figure 1
The levels of MDA and tGSH
in the ovarian tissue of the
study groups. *p<0.001
according to OIRD and sham
(SG) groups.
5. ever, severe hemorrhage observed in the OIR
group was not seen in the desloratadine group.
Furthermore, fluid accumulation in the subcap-
sular area and inflammatory cell infiltration were
not observed in the desloratadine group. A re-
duction in necrotic changes in the parenchymal
stroma, follicle structure, and corpus luteum was
reported (Figure 5).
Discussion
The effect of desloratadine on ovarian I/R dam-
age in animals was examined biochemically and
histopathologically in this study. Understanding
the biochemical mechanisms of a tissue and I/R
injury are very important in terms of developing
new treatment options to reduce tissue damage in
clinical practice.21 Biochemical studies have shown
that ROS such as superoxide anion (O-
2), hydrogen
peroxide (H2O2), and hydroxyl radical (OH) have
a significant role in the pathogenesis of I/R in-
jury.22 ROS initiate lipid peroxidation (LPO), which
causes cellular damage.23 The best known of the
various aldehydes resulting from LPO is MDA.
MDA causes crosslinking and polymerization of
membrane components. In addition, MDA causes
serious cellular damage by inactivating mem-
brane receptors, enzymes, and proteins.23,24 The
amount of MDA in ovarian tissue of animals
treated with desloratadine was found to be sig-
nificantly lower than that of the I/R and control
groups. These findings indicate that desloratadine
significantly suppresses I/R-related LPO reaction
in ovarian tissue. A previous study has also re-
ported that desloratadine inhibited MDA produc-
Volume 42, Number 2/April 2020 65
Desloratadine for Ovarian Ischemia-Reperfusion Injury
Figure 2
The levels of NF-κB, TNF-α,
and IL-1β in the ovarian
tissue of the study groups.
*p<0.0001 according to OIRD
and sham (SG) groups.
Figure 3 Cross section of normal corpus luteum (star) and
normal developing follicular structures (arrows) in ovarian tissue
of the sham group (H&E stain, ×100).
Figure 4 Ovarian tissue section showing diffuse congestion
(star), hemorrhage (block arrows), subcapsular fluid
accumulation and inflammatory cell infiltration (bidirectional
arrow), and necrotic changes (zigzag arrows) in ovarian tissue of
I/R-treated OIR animals (H&E stain, ×100).
6. tion.13 Sadowska-Woda et al noted that deslorata-
dine inhibited oxidative stress by inhibiting the
production of MDA in vitro.25 Living organ-
isms develop numerous antioxidant mechanisms
against the harmful effects of ROS. These mech-
anisms inhibit ROS production and eliminate the
harmful effects of ROS. GSH is one of the most
important and well-known anti
oxidant mecha-
nisms in living tissues. GSH, which is a cellular
tripeptide consisting of L-glutamate, L-cysteine,
and glycine,26 reacts with H2O2 and organic per-
oxides to chemically detoxify H2O2 and protects
cells from ROS damage.1 All this information indi-
cates that there is an increase in ROS production
in ovarian tissue treated with I/R. In many stud
ies it has been shown that the tGSH level de-
creases in tissues with high oxidant levels.18,27,28
In our study, desloratadine prevented the I/R-
dependent reduction of tGSH in ovarian tissue.
This has suggested that desloratadine inhibits
ROS-associated tGSH depletion in ovarian tissue.
Relevant data have also been found in the litera
ture showing that desloratadine inhibits tGSH con-
sumption due to oxidative stress.13 Furthermore,
data have shown that desloratadine suppresses
ROS production.29 Another study has shown that
desloratadine exhibits antioxidant activity.30 These
findings indicate that our experimental results are
consistent with the results of previous studies.
Proinflammatory cytokines such as IL-1β, TNF-
a, and NF-kB are known to play a role in the
pathogenesis of tissue I/R injury.6,7 Gene transcrip-
tion of a few proinflammatory cytokine signaling
pathways is controlled by NF-kB.31 In addition,
secretion of IL-1b and TNF-a can be stimulated
by NF-kB.5 Therefore, NF-kB is known as a tran-
scription factor for the expression of a series of
proinflammatory cytokines that play a critical role
in the regulation of inflammatory and immune
responses.32 Inhibition of NF-kB activation was
shown to reduce TNF-a, IL-6, and IL-1b mRNA
levels.33 The levels of IL-1b, TNF-a, and NF-kB
increased in the I/R-treated ovarian tissue as
compared to that of the desloratadine and sham
groups in our study. These results indicate that
desloratadine exerts both antioxidant and anti-
inflammatory effects in ovarian tissue. Roumes-
tan et al also argue that desloratadine inhibits
NF-kB production.11 Inhibition of both basal and
induced NF-kB by desloratadine12 has noted that
it is an NF-kB inhibitor. In their study, Chen et
al stated that the strong antiallergic and anti-
inflammatory properties of desloratadine are due
to the blockage of the NF-kB pathway.34 Severe
histopathological symptoms such as diffuse con-
gestion and hemorrhage, sporadic accumulation
of fluid in the subcapsular area, inflammatory cell
infiltration, and necrosis in parenchymal stroma
and follicular structures were found in I/R-treated
ovarian tissue, where oxidant and proinflamma-
tory cytokines showed a significant increase. How-
ever, histopathological findings were much mild
er in the desloratadine group, where oxidant and
cytokine levels were low. Congestion and hem-
orrhage are common histopathologic symptoms
in ovarian I/R injury. Turkler et al showed that
marked congestion and hemorrhage developed in
I/R-treated ovarian tissue with increased oxidant
and proinflammatory parameters.16 Unlubilgin et
al reported that in addition to congestion and
hemorrhage, an I/R event caused inflammatory
cell accumulation and necrosis in ovarian tissue.18
In a study by Demiryilmaz et al, histopathological
findings similar to the results of our study were
found in I/R-associated oxidative ovarian injury.35
Soyman et al also reported the development of
some serious histopathological changes (follicular
degeneration, perinuclear edema and degeneration
in follicular granulosa cells and corpus luteum
cells), with the exception of congestion and hemor-
rhage in I/R procedure in ovarian tissue.36
66 Analytical and Quantitative Cytopathology and Histopathology®
Kadıoglu et al
Figure 5 Ovarian tissue section showing mild congestion (star),
subcapsular area with no fluid accumulation and inflammatory
cell infiltrate (bidirectional arrow), and mild necrotic changes
in parenchymal stroma (black arc) and in follicular structures
(arrow) and corpus luteum (block arrow) in ovarian tissue of
desloratadine-treated OIRD group (H&E stain, ×100).
7. In conclusion, I/R procedure increased oxida-
tive stress and the levels of proinflammatory mark-
ers in ovarian tissue in a rat model. Severe histo
pathological damage was found in the ovarian
tissue where oxidant and proinflammatory mark
ers were increased. Desloratadine was found to
suppress the elevation in I/R-associated oxidant
and proinflammatory cytokine levels in ovarian
tissue and alleviate histopathological damage. This
suggests that desloratadine may be beneficial in
the treatment of ovarian I/R injury.
References
1. Suleyman H, Gul V, Erhan E: Oxidative stress and tissue
damage. Erzincan Medical Journal 2018;1:1-4
2. Newmeyer DD, Ferguson-Miller S: Mitochondria: Releasing
power for life and unleashing the machineries of death. Cell
2003;112:481-490
3. Lindsay TF, Liauw S, Romaschin AD, Walker PM: The effect
of ischemia/reperfusion on adenine nucleotide metabolism
and xanthine oxidase production in skeletal muscle. J Vasc
Surg 1990;12:8-15
4. Ha H, Yu MR, Choi YJ, Kitamura M, Lee HB: Role of high
glucose-induced nuclear factor-kappaB activation in mono-
cyte chemoattractant protein-1 expression by mesangial
cells. JASN 2002;13:894-902
5. Toby L: The nuclear factor NF-κB pathway in inflammation.
Cold Spring Harb Symp Quant Biol 2009;1:a001651
6. Beyazit F, Buyuk B, Turkon H, Elmas S, Uzun M: Adalim-
umab mitigates ovarian ischemia-reperfusion injury in rats
by regulating oxidative stress, apoptosis and resolution of
inflammation. J Obstet Gynaecol Res 2019;45:358-367
7. Nayki C, Nayki U, Keskin Cimen F, Kulhan M, Yapca,
OE, Kurt N, Bilgin Ozbek A: The effect of rutin on ovarian
ischemia-reperfusion injury in a rat model. Gynecol Endoc-
rinol 2018;34:809-814
8. Hibbard LT: Adnexal torsion. Am J Obstet Gynecol 1985;152:
456-461
9. Bayer AI, Wiskind AK: Adnexal torsion: can the adnexa be
saved? Am J Obstet Gynecol 1994;171:1506-1510
10. Henz BM: The pharmacologic profile of desloratadine: A
review. Allergy 2001;56(Suppl 65):7-13
11. Roumestan C, Henriquet C, Gougat C, Michel A, Bichon
F, Portet K, Jaffuel D, Mathieu M: Histamine H1-receptor
antagonists inhibit nuclear factor-kappaB and activator
protein-1 activities via H1-receptor-dependent and -inde-
pendent mechanisms. Clin Exp Allergy 2008;38:947-956
12. Wu RL, Anthes JC, Kreutner W, Harris AG, West RE, Jr: Des-
loratadine inhibits constitutive and histamine-stimulated
nuclear factor-kappaB activity consistent with inverse ago-
nism at the histamine H1 Receptor. I Int Arch Allergy Immu-
nol 2004;135:313-318
13. Tatar A, Parlak SN, Yayla M, Ugan RA, Polat E, Halici Z:
Effects of allergic rhinitis and desloratadine on the sub-
mandibular gland in a rat allergy model. Int Forum Allergy
Rhinol 2015;5:1164-1169
14. Kilic K, Sakat MS, Yildirim S, Kandemir FM, Gozeler MS,
Dortbudak MB, Kucukler S: The amendatory effect of
hesperidin and thymol in allergic rhinitis: An ovalbumin-
induced rat model. Eur Arch Otorhinolaryngol 2019;276:
407-415
15. Songu M, Ozkul Y, Kirtay S, Arslanoglu S, Ozkut M, Inan
S, Onal K: Effects of oral intake of cetirizine HCl and deslo-
ratadine molecules on the middle ear mucosa: An experi-
mental animal study. Eur Arch Otorhinolaryngol 2014;271:
833-838
16. Turkler C, Kulhan NG, Ata N, Kiremitli T, Cimen FK,
Suleyman H: The ameliorative effect of lutein on ovarian
ischemia-reperfusion injury in rats. Bratisl Lek Listy 2018;
119:713-717
17. Pansky M, Smorgick N, Herman A, Schneider D, Halperin R:
Torsion of normal adnexa in postmenarchal women and risk
of recurrence. Obstet Gynecol 2007;109:355-359
18. Unlubilgin E, Suleyman B, Balci G, Atakan Al R, Cankaya
M, Arslan Nayki U, Suleyman H: Prevention of infertility
in
duced by ovarian ischemia reperfusion injury by benidi-
pine in rats: Biochemical, gene expression, histopathological
and immunohistochemical evaluation. J Gynecol Obstet
Hum Reprod 2017;46:267-273
19. Ohkawa H, Ohishi N, Yagi K: Assay for lipid peroxides in
animal tissues by thiobarbituric acid reaction. Anal Biochem
1979;95:351-358
20. Sedlak J, Lindsay RH: Estimation of total, protein-bound,
and nonprotein sulfhydryl groups in tissue with Ellman’s
reagent. Anal Biochem 1968;25:192-205
21. Homer-Vanniasinkam S, Crinnion JN, Gough MJ: Post-
ischaemic organ dysfunction: A review. Eur J Vasc Endovasc
Surg 1997;14:195-203
22. Toyokuni S: Reactive oxygen species-induced molecular
damage and its application in pathology. Pathol Int 1999;49:
91-102
23. Girotti AW: Lipid hydroperoxide generation, turnover, and
effector action in biological systems. J Lipid Res 1998;39:1529-
1542
24. Goulart M, Batoreu MC, Rodrigues AS, Laires A, Rueff J:
Lipoperoxidation products and thiol antioxidants in chro
mium exposed workers. Mutagenesis 2005;20:311-315
25. Sadowska-Woda I, Sychta B, Rachel M, Bieszczad-Bedrejczuk
E: Protective effect of desloratadine against oxidative stress
in human erythrocytes in vitro. Environ Toxicol Pharmacol
2010;30:141-146
26. Murray RK, Granner DK, Mayes PA, Rodwell VM: Harper’s
Biochemistry. Twenty-fifth edition. USA, McGraw-Hill, 2000
27. Ingec M, Isaoglu U, Yilmaz M, Calik M, Polat B, Alp HH,
Kurt A, Gundogdu C, Suleyman H: Prevention of ischemia-
reperfusion injury in rat ovarian tissue with the on-off
method. J Physiol Pharmacol 2011;62:575-582
28. Isaoglu U, Yilmaz M, Sener E, Cetin N, Altuner D, Bilen H,
Yapca O, Demiryilmaz I, Isaoglu N, Gul M, Kumbasar S,
Suleyman H: The impaired balances of oxidant/antioxidant
and COX-1/COX-2 in ovarian ischemia-reperfusion injury
and prevention by nimesulide. Latin American Journal of
Pharmacy 2012;31:1481-1488
29. Cassano N, Raho G, Filieri M, D’Argento V, Amoruso A,
Volume 42, Number 2/April 2020 67
Desloratadine for Ovarian Ischemia-Reperfusion Injury
8. Filotico R, Vena GA: Influence of desloratadine on oxidative
stress markers in patients with chronic idiopathic urticaria.
Int J Dermatol 2006;45:394-396
30. Maouia A, Youssef M, Leban N, Ben Chibani J, Helal AN,
Kassab A: CRP relevance in clinical assessment of chronic
spontaneous urticaria Tunisian patients. Cutan Ocul Toxicol
2017;36:387-392
31. McDaniel DK, Eden K, Ringel VM, Allen IC: Emerging roles
for noncanonical NF-kappaB signaling in the modulation of
inflammatory bowel disease pathobiology. Inflamm Bowel
Dis 2016;22:2265-2279
32. Godowski PJ: A smooth operator for LPS responses. Nat
Immunol 2005;6:544-546
33. He X, Liu W, Shi M, Yang Z, Zhang X, Gong P: Docosa
hexaenoic acid attenuates LPS-stimulated inflammatory
response by regulating the PPARgamma/NF-kappaB path-
ways in primary bovine mammary epithelial cells. Res Vet
Sci 2017;112:7-12
34. Chen M, Xu S, Zhou P, He G, Jie Q, Wu Y: Desloratadine
citrate disodium injection, a potent histamine H(1) receptor
antagonist, inhibits chemokine production in ovalbumin-
induced allergic rhinitis guinea pig model and histamine-
induced human nasal epithelial cells via inhibiting the
ERK1/2 and NF-kappa B signal cascades. Eur J Pharmacol
2015;767:98-107
35. Demiryilmaz I, Sener E, Cetin N, Altuner D, Akcay F,
Suleyman H: A comparative investigation of biochemical
and histopathological effects of thiamine and thiamine
pyrophosphate on ischemia-reperfusion induced oxidative
damage in rat ovarian tissue. Arch Pharm Res 2013;36:1133-
1139
36. Soyman Z, Kelekci S, Sal V, Sevket O, Bayindir N, Uzun H:
Effects of Apigenin on experimental ischemia/reperfusion
injury in the rat ovary. Balkan Med J 2017;34:444-449
68 Analytical and Quantitative Cytopathology and Histopathology®
Kadıoglu et al