IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Insecticidal Activity of Stem Bark Extract of Lophira Alata Ekki Against Cowp...ijtsrd
Methanolic extract of Lophira alata was evaluated for its efficacy as contact and fumigant insecticides on cowpea bruchid, Callosobruchus maculatus in the laboratory at ambient tropical conditions of temperature and relative humidity. The plant powder tested was applied at rates 0.0 control , 2.0 g and 3.0 g 20 g of cowpea seeds either directly for contact with the insect pest or in plastic containers to assess its fumigant toxicity. Results of contact toxicity assay showed that powders of L. alata was effective against the adult C. maculatus causing 90 mortality 4.00 ± 0.57 within 2 days of application at 3.0 g 20 g of cowpea seeds as compared with 90 mortality 5.38 ± 0.50 recorded on day 4 of 2.0 g concentration application. The results of fumigant assays showed that L. alata had the highest insecticidal activity causing 95 mortality of C. maculatus within 4 days of application at rate 3.0 g 20g of cowpea seeds in contrast to 80 mortality recorded in 96 hrs of 2.0g concentration application. The phytochemical screening of the plant revealed alkaloids, saponins, glycosides, phytosterols, tannis, flavonoids and terpenoids while reducing sugar was absent. This study showed that the tested plant product is toxic to cowpea bruchid and the powders can be mixed with cowpea seeds to prevent hatching of the eggs thereby helping in their management. Ifelolu A. Remi-Esan | Olusola O. Bankole "Insecticidal Activity of Stem Bark Extract of Lophira Alata (Ekki) Against Cowpea Bruchid (Callosobruchus Maculatus)" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-4 , June 2020, URL: https://www.ijtsrd.com/papers/ijtsrd31207.pdf Paper Url :https://www.ijtsrd.com/biological-science/other/31207/insecticidal-activity-of-stem-bark-extract-of-lophira-alata-ekki-against-cowpea-bruchid-callosobruchus-maculatus/ifelolu-a-remiesan
Plant Tissue culture part II by Dr. Preeti VermaPreeti Verma
This presentation is meant only for educational purpose and includes various aspects of Plant Tissue culture in brief, including Media, Requirements, Problems in PTC, Techniques, Basic requirements for PTC LAB, Adavantages and Applications
Effect of Nitrobenzene granules and Seaweed extracts on biochemical contents ...Agriculture Journal IJOEAR
The present study is aimed to evaluate the effect of organic extracts (benzene, diethyl ether and water) of seaweeds (Halimeda gracilis, Ceramium rubrum and Cystophyllum muricatum) and nitrobenzene granuleson biochemical contents of Arachishypogea L. callusunder in vitro conditions. The callus of Arachishypogea L. was obtained from the leaf explants on MS medium containing 2, 4-D (1 mgL-1) and BAP (0.5 mg L-1). The mass multiplication of callus was achieved at 1mg L-1 of 2, 4-D and 0.5 mg L-1 of GA3. The calli were then treated with different concentrations (0.5, 1.0 and 1.5 mg L-1) of seaweed extracts and Nitrobenzene granules. Total carbohydrate, total protein and total chlorophyll contents were analyzed at 5, 10 and 15 days intervals. The total carbohydrate content was high (3.7mg/100mg) in callus treated with Benzene extract of Ceramium rubrumat 1.5 mg L-1 on 15th day. The total protein content was increased (6.9mg/100 mg) in callus treated with Benzene extract of Cystophyllum muricatum at 0.5 mg L-1 on 5th day and the total chlorophyll content was lower (0.36mg/100mg) in Nitrobenzene granules at 0.5mg L-1 in 5th day when compare to control. The present study reveals the positive role of different extracts of seaweeds on increasing the biochemical contents of callus culture of A.hypogea. The extracts can be further evaluated for their role on enhanced regeneration of plants from callus culture.
In vitro Shoot Micro Propagation of Medicinal Applications and Ornamental Val...Shafkat Shamim Rahman
Cestrum nocturnum L. a night Blooming jasmine belongs to Solanaceae family widely circulated all over tropical as well as subtropical areas of the World. It is mainly popular for ornamental fragrant flowering and hedge plant but also sometimes for traditional medicinal purpose. Due to strongest smelling characteristics of the plants, it is used in many industries for making Perfumes, essential Oils, Soaps, Candles, Body Oils, etc. The existence of natural plants of economic importance are threatened due to rapid urban development, including industrialization, residential development, educational, commercial etc., reduce the land for cultivation. Hence plant tissue culture protocol may be adapted for production and utilization of economically popular plants, including C. nocturnum involving limited space and short period of time. Shoot tip explants of naturally grown C. nocturnum were excised sterilized and endued on ‘Murashige and Skoog’ (MS) medium enriched changed concentration of BA, NAA, as well as GA3 singly or in combination. Excised micro shoots were examined for root development on 0.5 MS using IBA, NAA as well as IAA separately. The highest amount of multiple bud were observed in low concentration of BA (01.50 milligram × l-1), resulted no. of shoot 4.40 as well as 4.20/explant, no. of leaves 15.40 as well as 4.20/explant as well as size of different shoot 5.360 as well as 4.860 cm. The concentration of IBA and IAA were found to be best for root formation in micro shoots (13.20, 6.80 roots/micro shoots) as well as root size (8.39, 5.73 cm) individually. There are many opportunity of plant tissue culture which offer marvelous chances in plant propagation, plant development as well as creation of plants with necessary agronomical features. Finally often hardening plantlets were gradually adjusted to natural condition and acclimatized with 90% success. This established protocol could help plant cell biotechnology, horticulture, medical and industrial sector of the country.
This study establishs a basis for growing
plantlets without sucrose and investigating other
factors like carbon dioxide and light regime to
improve the in vitro growth performance.
Comparative Study of Production of Single Cell Protein from Different Agricul...ijtsrd
Single cell protein SCP also referred as microbial protein is defined as protein derived from cells of microorganisms such as yeast, fungi, algae, and bacteria, which are grown on various carbon sources for synthesis. The dried cells of microorganisms or the whole organism is harvested and consumed. In this work SCP was produced from different agricultural waste substrates like food and vegetable waste, rice husk, pulses husk, bagasse and wheat straw using Aspergillus niger. These substrates not only act as nutritive supplement but also ensure good waste management. Also, carbohydrate content of each sample was determined. For maximizing the yield of SCP, some factors were optimized. Various buffers were used like phosphate buffer, carbonate bicarbonate buffer and 0.1N NaOH. The sample that shows the best result for SCP was identified to be MCD and fruit and vegetable waste in 50 50 ratio and rice husk. In the future SCP could be produced to not only be used to produce protein but multiple products rich in carbohydrate, vitamins, lipids and other amino acids. Also yield could be increased by genetically modifying SCP organisms. Abhishikta Dasgupta | Jasmine Chughasrani "Comparative Study of Production of Single Cell Protein from Different Agricultural Waste Substrates using Aspergillus Niger" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-2 , February 2021, URL: https://www.ijtsrd.com/papers/ijtsrd38339.pdf Paper Url: https://www.ijtsrd.com/home-science/food-biotechnology/38339/comparative-study-of-production-of-single-cell-protein-from-different-agricultural-waste-substrates-using-aspergillus-niger/abhishikta-dasgupta
Plant Tissue Culture (Nucleic Acids, Amino Acids, Callus Culture, Transgenic Plants, Embryo Rescue, Embryonic Tissues, Cometabolism, Fungi and Actinomycetes, Grampositive Rods, Cloning Vectors, Biodegradation, Batch Cultures, Organ Culture)
Plants cell tissue culture is a rapidly developing technology which holds promise of restructuring agricultural and forestry practices. During the last two decades cell culture have made considerable advanced in the field of agriculture, horticulture, plant breeding, forestry, somatic cell genetics, phytopathology etc. Plant cells can be grown in isolation from intact plants in tissue culture systems. The cells have the characteristics of callus cells, rather than other plant cell types. These are the cells that appear on cut surfaces when a plant is wounded and which gradually cover and seal the damaged area.
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New Delhi-110007, India.
Email: npcs.ei@gmail.com , info@entrepreneurindia.co
Tel: +91-11-23843955, 23845654, 23845886, 8800733955
Mobile: +91-9811043595
Website: www.entrepreneurindia.co , www.niir.org
Tags
Plant Tissue Culture in India, Commercialization of Plant Tissue Culture in India, Role of Plant Tissue Culture in Agriculture, Plant Tissue Culture Industry in India, Industrial Plant Tissue Culture, Tissue Culture in Agriculture, Plant Tissue Culture, Tissue Culture, Cell Culture and Tissue Culture, Tissue Culture and Cell Culture, Tissue Culture in Plants, Plant Cell and Tissue Culture, Commercial Plant Tissue Culture in India, Plant Tissue Culture Business Plan, Plant Tissue Culture and Biotechnology, Tissue Culture Plants, Plant Tissue Culture Business Plan, Business Opportunities in Plant Tissue Culture, Tissue Culture Methods, Cybrid Production, Process of Cybrids Production, Production of Cybrids, Production of Cybrid Plants, Production of Haploid Plants, Haploid Production, Plant Secondary Metabolism, Production of Secondary Metabolites, Production of Secondary Metabolites Using Plant Cell Cultures, Plant Tissue Cultures in Production of Secondary Metabolites, Secondary Metabolites Production, Production of Somatic Hybrid Plants, Somatic Hybridization of Plants, Somatic Hybrid, Somatic Hybrid Production, Production of Enriched Biomass, Enrichment on Biomass Production, Formulation of Tissue Culture Medium, Collection of Explant Materials, Subculture of Callus, Regeneration of Plants from Callus, Preparation of Chick Embryo Extract, Preparation of Embryo Extract from Young Embryos, Preparation of Bovine Embryo Extract, Preparation of Eagles Medium, Media for Plant Tissues, Organ Culture, Preparation of Trypsinised Embryonic Carcass, Enrichment Culture Methods, Genetic Modification of Industrial Microorganisms Mutation
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
A Prelimnary Survey on TheAbundance of Mosquito Species and Transmission of P...iosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Sự có mặt khắp mọi nơi của smartphone đang tạo ra nhiều cơ hội hơn cho marketing qua di động cũng như tạo ra nhiều nhu cầu về nguồn marketing qua di động.
Insecticidal Activity of Stem Bark Extract of Lophira Alata Ekki Against Cowp...ijtsrd
Methanolic extract of Lophira alata was evaluated for its efficacy as contact and fumigant insecticides on cowpea bruchid, Callosobruchus maculatus in the laboratory at ambient tropical conditions of temperature and relative humidity. The plant powder tested was applied at rates 0.0 control , 2.0 g and 3.0 g 20 g of cowpea seeds either directly for contact with the insect pest or in plastic containers to assess its fumigant toxicity. Results of contact toxicity assay showed that powders of L. alata was effective against the adult C. maculatus causing 90 mortality 4.00 ± 0.57 within 2 days of application at 3.0 g 20 g of cowpea seeds as compared with 90 mortality 5.38 ± 0.50 recorded on day 4 of 2.0 g concentration application. The results of fumigant assays showed that L. alata had the highest insecticidal activity causing 95 mortality of C. maculatus within 4 days of application at rate 3.0 g 20g of cowpea seeds in contrast to 80 mortality recorded in 96 hrs of 2.0g concentration application. The phytochemical screening of the plant revealed alkaloids, saponins, glycosides, phytosterols, tannis, flavonoids and terpenoids while reducing sugar was absent. This study showed that the tested plant product is toxic to cowpea bruchid and the powders can be mixed with cowpea seeds to prevent hatching of the eggs thereby helping in their management. Ifelolu A. Remi-Esan | Olusola O. Bankole "Insecticidal Activity of Stem Bark Extract of Lophira Alata (Ekki) Against Cowpea Bruchid (Callosobruchus Maculatus)" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-4 , June 2020, URL: https://www.ijtsrd.com/papers/ijtsrd31207.pdf Paper Url :https://www.ijtsrd.com/biological-science/other/31207/insecticidal-activity-of-stem-bark-extract-of-lophira-alata-ekki-against-cowpea-bruchid-callosobruchus-maculatus/ifelolu-a-remiesan
Plant Tissue culture part II by Dr. Preeti VermaPreeti Verma
This presentation is meant only for educational purpose and includes various aspects of Plant Tissue culture in brief, including Media, Requirements, Problems in PTC, Techniques, Basic requirements for PTC LAB, Adavantages and Applications
Effect of Nitrobenzene granules and Seaweed extracts on biochemical contents ...Agriculture Journal IJOEAR
The present study is aimed to evaluate the effect of organic extracts (benzene, diethyl ether and water) of seaweeds (Halimeda gracilis, Ceramium rubrum and Cystophyllum muricatum) and nitrobenzene granuleson biochemical contents of Arachishypogea L. callusunder in vitro conditions. The callus of Arachishypogea L. was obtained from the leaf explants on MS medium containing 2, 4-D (1 mgL-1) and BAP (0.5 mg L-1). The mass multiplication of callus was achieved at 1mg L-1 of 2, 4-D and 0.5 mg L-1 of GA3. The calli were then treated with different concentrations (0.5, 1.0 and 1.5 mg L-1) of seaweed extracts and Nitrobenzene granules. Total carbohydrate, total protein and total chlorophyll contents were analyzed at 5, 10 and 15 days intervals. The total carbohydrate content was high (3.7mg/100mg) in callus treated with Benzene extract of Ceramium rubrumat 1.5 mg L-1 on 15th day. The total protein content was increased (6.9mg/100 mg) in callus treated with Benzene extract of Cystophyllum muricatum at 0.5 mg L-1 on 5th day and the total chlorophyll content was lower (0.36mg/100mg) in Nitrobenzene granules at 0.5mg L-1 in 5th day when compare to control. The present study reveals the positive role of different extracts of seaweeds on increasing the biochemical contents of callus culture of A.hypogea. The extracts can be further evaluated for their role on enhanced regeneration of plants from callus culture.
In vitro Shoot Micro Propagation of Medicinal Applications and Ornamental Val...Shafkat Shamim Rahman
Cestrum nocturnum L. a night Blooming jasmine belongs to Solanaceae family widely circulated all over tropical as well as subtropical areas of the World. It is mainly popular for ornamental fragrant flowering and hedge plant but also sometimes for traditional medicinal purpose. Due to strongest smelling characteristics of the plants, it is used in many industries for making Perfumes, essential Oils, Soaps, Candles, Body Oils, etc. The existence of natural plants of economic importance are threatened due to rapid urban development, including industrialization, residential development, educational, commercial etc., reduce the land for cultivation. Hence plant tissue culture protocol may be adapted for production and utilization of economically popular plants, including C. nocturnum involving limited space and short period of time. Shoot tip explants of naturally grown C. nocturnum were excised sterilized and endued on ‘Murashige and Skoog’ (MS) medium enriched changed concentration of BA, NAA, as well as GA3 singly or in combination. Excised micro shoots were examined for root development on 0.5 MS using IBA, NAA as well as IAA separately. The highest amount of multiple bud were observed in low concentration of BA (01.50 milligram × l-1), resulted no. of shoot 4.40 as well as 4.20/explant, no. of leaves 15.40 as well as 4.20/explant as well as size of different shoot 5.360 as well as 4.860 cm. The concentration of IBA and IAA were found to be best for root formation in micro shoots (13.20, 6.80 roots/micro shoots) as well as root size (8.39, 5.73 cm) individually. There are many opportunity of plant tissue culture which offer marvelous chances in plant propagation, plant development as well as creation of plants with necessary agronomical features. Finally often hardening plantlets were gradually adjusted to natural condition and acclimatized with 90% success. This established protocol could help plant cell biotechnology, horticulture, medical and industrial sector of the country.
This study establishs a basis for growing
plantlets without sucrose and investigating other
factors like carbon dioxide and light regime to
improve the in vitro growth performance.
Comparative Study of Production of Single Cell Protein from Different Agricul...ijtsrd
Single cell protein SCP also referred as microbial protein is defined as protein derived from cells of microorganisms such as yeast, fungi, algae, and bacteria, which are grown on various carbon sources for synthesis. The dried cells of microorganisms or the whole organism is harvested and consumed. In this work SCP was produced from different agricultural waste substrates like food and vegetable waste, rice husk, pulses husk, bagasse and wheat straw using Aspergillus niger. These substrates not only act as nutritive supplement but also ensure good waste management. Also, carbohydrate content of each sample was determined. For maximizing the yield of SCP, some factors were optimized. Various buffers were used like phosphate buffer, carbonate bicarbonate buffer and 0.1N NaOH. The sample that shows the best result for SCP was identified to be MCD and fruit and vegetable waste in 50 50 ratio and rice husk. In the future SCP could be produced to not only be used to produce protein but multiple products rich in carbohydrate, vitamins, lipids and other amino acids. Also yield could be increased by genetically modifying SCP organisms. Abhishikta Dasgupta | Jasmine Chughasrani "Comparative Study of Production of Single Cell Protein from Different Agricultural Waste Substrates using Aspergillus Niger" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-2 , February 2021, URL: https://www.ijtsrd.com/papers/ijtsrd38339.pdf Paper Url: https://www.ijtsrd.com/home-science/food-biotechnology/38339/comparative-study-of-production-of-single-cell-protein-from-different-agricultural-waste-substrates-using-aspergillus-niger/abhishikta-dasgupta
Plant Tissue Culture (Nucleic Acids, Amino Acids, Callus Culture, Transgenic Plants, Embryo Rescue, Embryonic Tissues, Cometabolism, Fungi and Actinomycetes, Grampositive Rods, Cloning Vectors, Biodegradation, Batch Cultures, Organ Culture)
Plants cell tissue culture is a rapidly developing technology which holds promise of restructuring agricultural and forestry practices. During the last two decades cell culture have made considerable advanced in the field of agriculture, horticulture, plant breeding, forestry, somatic cell genetics, phytopathology etc. Plant cells can be grown in isolation from intact plants in tissue culture systems. The cells have the characteristics of callus cells, rather than other plant cell types. These are the cells that appear on cut surfaces when a plant is wounded and which gradually cover and seal the damaged area.
See more
https://goo.gl/pXccQD
https://goo.gl/MNnSqw
https://goo.gl/QgJiqW
Contact us:
Niir Project Consultancy Services
106-E, Kamla Nagar, Opp. Spark Mall,
New Delhi-110007, India.
Email: npcs.ei@gmail.com , info@entrepreneurindia.co
Tel: +91-11-23843955, 23845654, 23845886, 8800733955
Mobile: +91-9811043595
Website: www.entrepreneurindia.co , www.niir.org
Tags
Plant Tissue Culture in India, Commercialization of Plant Tissue Culture in India, Role of Plant Tissue Culture in Agriculture, Plant Tissue Culture Industry in India, Industrial Plant Tissue Culture, Tissue Culture in Agriculture, Plant Tissue Culture, Tissue Culture, Cell Culture and Tissue Culture, Tissue Culture and Cell Culture, Tissue Culture in Plants, Plant Cell and Tissue Culture, Commercial Plant Tissue Culture in India, Plant Tissue Culture Business Plan, Plant Tissue Culture and Biotechnology, Tissue Culture Plants, Plant Tissue Culture Business Plan, Business Opportunities in Plant Tissue Culture, Tissue Culture Methods, Cybrid Production, Process of Cybrids Production, Production of Cybrids, Production of Cybrid Plants, Production of Haploid Plants, Haploid Production, Plant Secondary Metabolism, Production of Secondary Metabolites, Production of Secondary Metabolites Using Plant Cell Cultures, Plant Tissue Cultures in Production of Secondary Metabolites, Secondary Metabolites Production, Production of Somatic Hybrid Plants, Somatic Hybridization of Plants, Somatic Hybrid, Somatic Hybrid Production, Production of Enriched Biomass, Enrichment on Biomass Production, Formulation of Tissue Culture Medium, Collection of Explant Materials, Subculture of Callus, Regeneration of Plants from Callus, Preparation of Chick Embryo Extract, Preparation of Embryo Extract from Young Embryos, Preparation of Bovine Embryo Extract, Preparation of Eagles Medium, Media for Plant Tissues, Organ Culture, Preparation of Trypsinised Embryonic Carcass, Enrichment Culture Methods, Genetic Modification of Industrial Microorganisms Mutation
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
A Prelimnary Survey on TheAbundance of Mosquito Species and Transmission of P...iosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Sự có mặt khắp mọi nơi của smartphone đang tạo ra nhiều cơ hội hơn cho marketing qua di động cũng như tạo ra nhiều nhu cầu về nguồn marketing qua di động.
Evaluation of Some Prominent Biochemical Agents in Menstrual Phases of Womeniosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Molecular Identification of Specific Virulence Genes in EnteropathogenicEsche...iosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
www.sdat.tn.gov.in Jobs 2015-16 For Coach 38 Vacancies Application FormPriya Jain
Sports Development Authority of Tamil Nadu has circulated an advertisement for filling up the 38 vacancies of Coach through direct recruitment. More details- http://recruitmentresult.com/sdat-recruitment/
Pesticide Contamination in some lakes of Rajasthaniosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Callus Induction and Shoot Regeneration in VIGNA RADIATAijsrd.com
Plant Tissue Culture is a practice used to propagate plants under sterile conditions, often to produce clones of a plant. Different techniques in plant tissue culture may offer certain advantages over traditional methods of propagation. We have taken the Vigna radiata seeds as explant for callus induction and shoot regeneration. Because Mungbean is a food grain, legume crop all over the world. This crop is regarded as a quality pulse in India for its excellent protein and high digestibility. Several biotic and abiotic factors as well as low genetic variability are supposed to be responsible for low production of this crop. Explant was sterilized and inoculated on callus induction and shoot regeneration medium separately supplemented with hormones. The medium used for callus induction includes MS medium and other hormones like 2,4-D and Kinetin and medium used for shoot regeneration includes MS medium and other hormones like Kinetin and BAP and the explants were incubated in tissue culture lab under aseptic conditions and light and temperature of 25 ± 20C was provided. After first week, discolorations of explants were observed, after 3 weeks small proliferations appeared on the explant surface. The undifferentiated mass of cells i.e. callus is developed after 5 weeks. In shoot regeneration culture tubes after 2 weeks leaf primordia was observed, and the differentiation and elongation of shoots were observed during 6 weeks.
Banana is the fourth largest produced food crop of the world and its demand is increasing day by day. It is available throw out the year and its cost is very less in comparison to other fruits. With the development in science new tissue culture protocols are standardized for mass propagation of Musa (Banana) on the basis of effects of plant growth regulators. BAP (6-Benzyl Amino Purine), KN (Kinetin) are most widely used cytokinins for shoot proliferation and IAA (Indole -3-acetic acid), NAA (Naphathalene acetic acid) are widely used auxins for root induction.
The experiment was conducted at the experimental farm and laboratory of Institute of
Sustainable Agrotechnology, University Malaysia Perlis, Padang Besar, Perlis, Malaysia, with the objective
toinvestigate the inhibitory and stimulatory effects of aqueous extract of mungbean on seed germination and
seedling growth of three crop species, mungbean, sweet corn and okra. Different treatments of mungbean
aqueousextracts (vegetative fresh, vegetative after 2 weeks drying, vegetative after 4 weeks drying, flowering
fresh, flowering after 2 weeks drying, flowering after 4 weeks drying, flowering fresh, flowering after 2 weeks
drying, flowering after 4 weeks drying, maturity fresh, maturity after 2 weeks drying, maturity after 4 weeks
drying and water as control) were used to test their effect on the test species. The experiment was randomly
distributed and according to Completely Randomized Design(CRD) with five replicates. The results showed the
fresh vegetative aqueous extract of mungbean had a significant effect (stimulatory) on germination percent and
growth parameters such as number of root, root length and shoot height, of the three crop species. The study
revealed that the aqueous extract of mungbean have different effects (inhibitory and stimulatory) on the
seedlings and the mode of action depends on the associated plant species.Our results suggest that the aqueous
extract of mungbean from the different growth stages and drying periods have an allelopathic effect.
The Studies of Effect of Bio Fertilizers Rhizobium, Phosphobacteria, and Root...IIJSRJournal
A pot culture experiment was carried out and to the find out the effect of Rhizobium, Phosphobacteria and Root Nodule extract on the vegetative growth and biochemical changes in Black gram and Maize. The Black gram and Maize is vegetative parameters such as seed germination, shoot and root length, leaf fresh weight, leafs dry weight, shoot and root dry weight had analyzed and biochemical studies of both Black gram and Maize was carried such as total chlorophyll content, leave soluble protein and in vivo nitrate reductase activity. Among microbial inoculants, the Rhizobium + PB mixer was found most effective in terms of seed germination, shoot and root length, leaf fresh weight, leafs dry weight, shoot and root dry weight and also showed increase total chlorophyll content, soluble protein and nitrate activities in both Black gram and Maize.
Yield Performances of Pleurotus ostreatus on Different Growth Substrates as I...IOSRJPBS
This study was carried out to investigate the effects of some vegetables (Diplazium sammatii, Moringa oleifera, and Justicia insularis) as additives on the growth parameters of Pleurotus ostreatus cultivated on two substrates; banana leaves (BL) and banana leaves/sawdust (BL/SD). The substrates were amended with these vegetables at different concentration levels (0 g, 5 g, 10 g and 15 g). Spawn running was completed in 30, 35, and 40 days in banana leaves, banana leaves/sawdust and the control respectively. Parameters assessed included area of pileus, number of fruit bodies, fresh weight, dry weight, length of stipe and girth of stipe. Higher mean values for fresh weight (21.69 g), area of pileus (42.58 cm2 ), length of stipe (5.10 g) and girth of stipe (4.30 g) were obtained from BL substrate treated to 5 g M. oleifera leaf powder. These values were significantly different from values obtained for other plant additives. Considering the number of fruit bodiesthat emerged on BL/SD substrates, the control produced more fruit bodies than the substrates with additives. The best performance in BL/SD substrates was observed when the substrate was treated to 10 g J. insularis in some of the parameters assessed. These included fresh weight (20.64 g), number of fruit bodies (8.00) and stipe girth (3.44 cm).Other parameters like area of pileus (36.08 cm2 ) and dry weight (2.39 cm), control had a better performance. While for length of stipe, 15 g M. oleifera leaf powder had the highest mean value that was significantly different (P≤0.05) from what was observed in substrates amended with other additives. It is therefore evident that these vegetables have high potential for utilization as additives or supplements for growing of Pleurotus ostreatus especially when using BL as substrates. In addition, substratesadditive concentration combination played a significant role in selective organ growth of the test mushroom.
PLANT TISSUE CULTURE
K. Vanangamudi
History of plant tissue culture
Terms and terminology of plant tissue culture
Techniques of plant tissue culture
Stages of micro propagation
Diagrammatic representation of stages of micropropagation
Advantages of micro propagation
Demerits of micropropagation
Commercially propagated plants through micro propagation in India
Explants and medium used
Here, all information about Plant Tissue Culture
HISTORY OF PLANT TISSUE CULTURE
THE TECHNIQUE OF PLANT TISSUE CULTURE
Plantlet Regeneration and Transfer to Soil
A Classification of Tissue Culture Techniques
EMBRYO CULTURE
MERISTEM CULTURE
ANTHER OR POLLEN CULTURE
TISSUE AND CELL CULTURES
SOMATIC HYBRIDIZATION
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Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Economization of Datura Plant Using Planttissue Culture
1. IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)
e-ISSN: 2278-3008, p-ISSN:2319-7676. Volume 10, Issue 6 Ver. II (Nov - Dec. 2015), PP 87-90
www.iosrjournals.org
DOI: 10.9790/3008-10628790 www.iosrjournals.org 87 | Page
Economization of Datura Plant Using Planttissue Culture
Prof. Aarti Shastri And Mr. Abhishek Dubey
Department of Biotechnology
Abstract: The subsequent research deals with a fundamental plant tissue culture technique where by my
primary aim is to develop a callus of the Datura (innoxia spp.) plant. Following this, a comparative study of the
extractive values of the callus cultured with the help of Murashige and Skoog media and its alterations with
Datura plant was carried out. My other experiment with the newly developed callus included bringing about
variations in the media to find out the finest possible combination of nutrients required by the Datura
Extractions were performed by two different methods, both consisting of dissimilar organic solvents used for
extraction and different soaking times. The extractive values following the callus growth were calculated and
the essential aim of the experiment was to develop a callus with a higher extractive value than the original
explants (seed) yet with a retained therapeutic potency. For this the crystals obtained from both the methods
were weighed and there extractive values compared to the preparatory material were calculated. This helped us
get an understanding about the variations that needed to be made in the media to get a constructive resultant
callus growth. The extractive value obtained by the first method was the maximum. Hence the results were
favorable for the supplementary study to be persistent.
Keywords: Economization, Media, DaturaInnoxia, Plant Tissue Culture, Callus, Extractive Values.
I. Introducing
Plant tissue culture is a practice used to propagate plants under sterile conditions, often produce clones
of a plant and have several advantages over traditional methods such as: a) The production of multiples of plants
in the absence of seeds or necessary pollinators to produce seeds. b) The regeneration of whole plants from plant
cells that have been genetically modified.
DaturaInoxia consists of the dried leaves and flowering tops of DaturaInoxia Linn.Belonging to family
Solanaceae. Daturainoxia is an annual shrubby plant that typically reaches a height of 0.6 to 1.5 meters. The
flowers are white, trumpet-shaped, 12–19 cm long. The fruit is an egg-shaped spiny capsule, about 5 cm in
diameter. All parts of the plant are anodyne, antispasmodic, hallucinogenic, hypnotic and narcotic. It has been
used in the past as a pain killer and also in the treatment of insanity, fevers with catarrh, diarrhea and skin
diseases. The plant contains several alkaloids, the most active of which is scopolamine. This is a potent
cholinergic-blocking hallucinogen, which has been used to calm schizoid patients. The leaves contain 0.52%
scopolamine, the calices 1.08%, the stems 0.3%, the roots 0.39%, the fruits 0.77%, the capsules 0.33%, the
seeds 0.44% and the whole plant 0.52 - 0.62% The tissue which is obtained from the plant to culture is called an
explant. Explants are then usually placed on the surface of a Murashige and Skoog medium or (MSO or
MS0(MS-zero)), Which is a plant growth medium used in the laboratories for cultivation of plant cell culture
and composed of Macronutrients such as Ammonium nitrate ,Boric acid,Cobalt chloride,Magnesium
sulfate,Cupric sulfate, Potassium phosphate, Ferrous sulfate,Potassium nitrate ,Manganese sulfate, Potassium
iodide,Sodium molybdate ,Zinc sulfate and Na2EDTA · 2H2Oa.Common organic additives such as myo-
Inositol,Nicotinic acid,Pyridoxine hydrochloride,Thiaminehydrochloride,Glycine (recrystallized), Sucrose.
II. Materials And Methods
Prepararion of Media
Take 2g agar and 3.5g Murashige and Skoog media in a conical flask. Add distilled water and dilute to
100ml.Cover the flask with aluminum foil. Autoclave for 15 minutes after the first whistle. After autoclaving is
complete, keep the autoclave untouched for some time in order to ensure that all the pressure has been released.
Transfer the media to an aseptic area. Here the media was transferred in the culture tubes under the laminar
flow. Slants were prepared. The media was allowed to solidify before proceeding further.
Surface Sterilization
The next step involves sterilizing the parts of the plant that are to be used for callus formation.
The parts of the plant that were taken were- Shoot, Root tip, Leaf, Stem, Flower, Bud, Seed Sodium
hypochlorite solution was used to sterilize them. These explants were kept in the solution for about 5-10 minutes
and transferred to a Petri plate containing distilled water where they were washed and further cultured.
2. Economization Of Datura Plant Using Planttissue Culture
DOI: 10.9790/3008-10628790 www.iosrjournals.org 88 | Page
The seeds that were used for culturing were also of various types.
From a freshly cut fruit.
From a dried fruit that had burst open naturally.
From a dried fruit that had not yet burst open to disperse the seeds.
Culturing
Separate culture tubes were prepared and labeled for each of the explants, i.e. leaf, stem, shoot, root tip,
flower and bud and seeds. The explants are placed carefully on the media. The culture tubes are then sealed with
parafilm in order to prevent any kind of contamination. Light and dark conditions are maintained for the
explants so that normal growth can take place.
Sub Culturing
After some days of the initial culturing, the explants are then transferred to a fresh media. Sub culturing
is done in order to provide a continuous supply of nutrients to the explants so that they can show the required
growth. Other conditions same as the culturing.
Culturing Conditions
The cultured tubes are placed in an aseptic area. The temperature provided is room temperature.
Day and night conditions are maintained. The cultures are subjected to 12 hours light and 12 hours dark.
The plant DaturaInoxia was cultured in media containing various alterations.
Alteration 1
The explants placed in media containing 2g agar, 3.5g Murashige and Skoog and salt. Observations were
recorded with 7-10 day’s time intervals.
Alteration2
The explants were placed on media containing sugar. This was of two types-
1. The sugar was placed in the along with agar in Murashige and Skoog in the autoclave and was autoclaved.
2. The sugar was added after the agar and Murashige and Skoog were autoclaved, i.e., the sugar was not
autoclaved but directly added to the media after it was autoclaved and before it could solidify.
The observations were recorded with 7-10 day’s time intervals.
Alteration 3
The explants were cultured in a medium containing 2g agar, 3.5g Murashige and Skoog media and reconstituted
coconut milk and the observations were recorded with 7-10 day’s time intervals.
Alteration 4
The explants were cultured in a medium containing 2g agar, 3.5g Murashige and Skoog media and a fertilizer
containing amino acids, and sea weeds and the observations were recorded with 7-10 day’s time intervals.
Alteration 5
The explants were cultured in a media containing 2g agar, 3.5g Murashige and Skoog media and vitamin B
complex and the observations were recorded with 7-10 day’s time intervals.
Alteration 6
The explants were subject to media containing 2g agar, 3.5g Murashige and Skoog media and lysine extract and
the observations were recorded with 7-10 day’s time intervals.
Alteration 7
The explants were cultured in a media containing 2g agar, 3.5g Murashige and Skoog media and yeast extract
and the observations were recorded with 7-10 day’s time intervals.
Alteration 8
The explants were cultured in a media containing 2g agar, 3.5g Murashige and Skoog media and activated
charcoal and the observations were recorded with 7-10 day’s time intervals.
Alteration 9
The explants were cultured in a media containing 2g agar, 3.5g Murashige and Skoog media and an organic
extract containing amino acids and triterpenoids and the observations were recorded with 7-10 day’s time
intervals.
Alteration 10
The explants were cultured in a media containing 2g agar, 3.5g Murashige and Skoog media and glycine and the
observations were recorded with 7-10 day’s time intervals.
3. Economization Of Datura Plant Using Planttissue Culture
DOI: 10.9790/3008-10628790 www.iosrjournals.org 89 | Page
Alteration 11
The antifungal agent ketoconazole was added to some of the culture tubes that contained seed as the explant and
the observations were recorded with 7-10 day’s time intervals.
Once these alterations in the media were made the explants were cultured as before-
Callus initiation in Salt Callus initiation in salt,
Vitamin b complex and lysine
Figure 1:Callus Initiation
Fertilizer, Yeast extract, Vitamin B Complex, Lysine, Salt, Organic Extract, Fertilizer
Activated Charcoal
Figure 2: Culture Tubes
III. Results And Discussion
The various factors that encourage or retard the duration required for callus formation of DaturaInoxiaseeds
were studied and the observations obtained are mentioned in the following table.
SR.NO. ALTERATIONS RESULTS
1. Murashige and Skoog+ Agar+ Salt Initiation of callus growth was observed after 5 days.
2. a)Murashige and Skoog+ Agar+ autoclave Sugar No initiation of callus was observed.
b)Murashige and Skoog+ Agar+ non-autoclaved sugar No initiation of callus was observed
3. Murashige and Skoog+ Agar+ reconstituted coconut milk No initiation of callus was observed
4. Murashige and Skoog+ Agar+ Fertilizer containing amino acids and sea
weeds
No initiation of callus was observed
5. Murashige and Skoog+ Agar+ Vitamin B complex Initiation of callus was observed after 5 days
6. Murashige and Skoog+ Agar+ Lysine Slow initiation of callus was observed after 10 days
7. Murashige and Skoog+ Agar+ Yeast extract Slow initiation of callus was observed after 7 days
8. Murashige and Skoog+ Agar+ Activated Charcoal Slow initiation of callus was observed after 10 days
9. Murashige and Skoog+ Agar+ Organic extract containing amino acids and
tri-terpenoids
No initiation of callus was observed
10. Murashige and Skoog+ Agar+ Glycine No initiation of callus was observed
11. Murashige and Skoog+ Agar+ Ketoconazole No initiation of callus was observed
4. Economization Of Datura Plant Using Planttissue Culture
DOI: 10.9790/3008-10628790 www.iosrjournals.org 90 | Page
IV. Conclusion
With various combinations of the composition of media, we have observed that the variations-
1. Murashige and Skoog + Agar+ Salt
2. Murashige and Skoog+ Agar+ Vitamin B complex
3. Murashige and Skoog+ Agar+ Yeast Extract
4. Murashige and Skoog+ Agar+ Lysine
5. Murashige and Skoog+ Agar+ Activated Charcoal
The above mentioned combinations have been proved to be better as compared to the others. Further,
the most favorable explant for callus formation was found to be the seeds taken from the dried fruit. A lot of
scope is still there to exploit the rationality between various nutritional supplement and methods of sterilization
and effective technique of explant incubation. These studies will lead to economization of plant tissue culture
techniques and its application in agricultural utility.
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