The document describes micropropagation, a method of plant tissue culture used to rapidly produce new plants that are genetically identical to the parent plant. Micropropagation involves taking small explants from plants and growing them in sterile culture media in controlled conditions. Key advantages include producing many new plants from a small explant sample, in a short time period, with consistent characteristics. Common plant types propagated through micropropagation include fruits, ornamentals, vegetables, and crops. The document also discusses various factors that affect micropropagation success, including explant type and condition, culture media formulation, temperature, light, and plant growth hormones.

1. Group II
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2. a method to produce plant from a
little part of a plant, tissue or in
vitro cell culture
Plant that is developed by tissue
culture is a plant that has higher
commercial value
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3. Micropropagasi is used to produce new plant in a large
amount, short in time and has same characteristic with
mother plant
Example :
Fruits
: Citrus, apple, banana
Ornament plants : Orchids, crisan, tulip
Vegetables
: Tomato, paprika, cabbage, carrot
Crops
: Oil palm, cocoa
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4. 1. Explant has small size which can be used to produce
many plants within small space
2. Use a little work
3. Planting room is smaller than conventional
4. Nutrients consentrate, light, temperature, and other
factors can be controlled
5. Not often necessary safeguards
6. Not depending on the season
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5. 1. High operating costs
2. Requires expert
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6. 1.
Embriogenesis: a process that results in the
formation without pollination embryos from
somatic tissue.
2.
Organogenesis: The process of formation or
induction of cell structure of plant tissue and
form shoot or root primordia were evident
between the parent and the resulting
primordia.
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7. There are 2 Organogenesis:
1. Direct Organogenesis
2. Indirect Organogenesis
1. Meristem
2. Terminal bud
3. Adventitus bud
4. Axilar bud
5. Calus
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8. Group II
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9. 1.
2.
3.
4.
5.
6.
7.
8.
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Physiology of mother plant
Media
Carbon and energy
Phytohormon
Temperature
Light
Humidity
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10. a.
Season ; intake of plant
explants should pay attention to
the state of the season.
b.
Suitability Grow ; this relates to
the transfer of culture results to
the field.
c.
Age of the Mother Plant ; plants
were used as explants should as
plant in vegetative growth
phase.
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11. Use media to appropriate plants
that has great affect to culture
plants.
Macronutrient : N, P, K, Ca, Mg.
Micronutrients; Fe and S
Carbon source and energy in the
form of sucrose are carbohydrates
are given to the media.
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12. plant hormones that affect the
growth of culture.
Auxin Hormone ; for initiation
of callus, somatic embryo
formation and stimulate root
formation
Cytokines, the hormone is
needed to stimulate to form
somatic embryos and young
shoots.
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13. The optimum temperature
required by the plant
breeding process is 23 ° C
- 30 ° c.
Light
intensity
and
irradiation are too often
determine
the
tissue
culture propagation.
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14. High humidity is needed
to
avoid
drought.
Humidity
is
usually
about 70%-80%.
Improper storage space
will
result
in
the
production of shoots and
roots are not perfect.
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15. To obtain optimum results, explant should be
taken from healthy plants and lush at the
level of physiological development it.
Explant that is taken must be organ, the
young plant cells because at this time it is
spliting and developing actively.
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16. Komposisi Media MS (1962)
Unsur
Konsentrasi Media (Mg ɲ)
Jumlah Stok Mg/L
Kekuatan (X)
Volume stok yang dipipet
(ml)/L
Makro
NH4NO3
1.650
KNO3
1.900
82.500
50
KH2PO4
170
95.000
50
MgSO4 7H2O
376
8.500
50
439,8
18.800
50
21.990
50
CaCl2 2H2O
20 ml
Mikro
MnSo4 4H2O
16,9
ZnSO4 7H2O
8,6
1.690
100
H3BO
6,2
860
100
KI
0,83
620
100
Na2Mo O4 2H2O
0,25
83
100
CuSO4 5H2O
0,025
25
100
CoCl2 6H2O
0,025
2,5
100
2,5
100
10 ml
Besi
FeSO4 7H2O
27,8
Na EDTA/Tritriplex
37,3
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2.780
3.730
16
100
100
10 ml
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17. Continued ...
Vitamin
Glycine
2,0
Pyridoxin-HCl
0,5
Thyamin-HCl
0,1
200
Nicotinic acid
0,5
50
10
100
100
100
1 ml
100
50
Myoinocitol
100
Sukrosa/glukosa
30 g
Phytagel/agar
7g
ZPT (IBA, BA 1, 2, 4, 6)
0,1-70 Mg/L
pH
5,8-6,0
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18. 1. Phase 0 : Selecting and preparing mother
plant
2. Phase 1 : Preparing culture and make a culture
strerility
3. Phase 2 : Preparing plant to moving
4. Phase 3 : Moving plant to outsite
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19. When we did practice in Islamic Riau University
exactly in
Biotechnology Laboratory at 7th November 2013, we just did until 2 steps,
they are:
Tahap 0 : Selecting and preparing mother plant
Tahap 1 : Preparing culture and make a culture strerility that is consist of
Sterility, Making Media and Inisiation.
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20. 1. Sterilisation
1.1 Rosella (Hibiscus sabdariffa. L) that has separated
from daging buah then, it was entered into sunlight
during 15 minutes and then it was washed by aquades
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21. Sterilisation
1.2 Same as Rosella, purple sweet potato (Ipomoea batatas
var ayumurasaki) was entered into sunlight and then washed
by aquades
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22. Sterilisation
1.3 Rosella (Hibiscus sabdariffa. L) was gave fungisida
and stired during 15 minutes and then washed by aquades
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23. Sterilisation
1.4 Purple sweet potato (Ipomoea batatas var ayumurasaki)
was peeled the surface and added fungisida during 15
minutes
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24. 2. Making Media
2.1 Process to entered macro and micro materials into
measuring glass in making media process
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25. Making Media
2.2 Aquades was added into solution that mixed by macro
and micro materials until 1000ml (left) and incorporate the
solution into the container material (right)
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26. Making Media
2.3 Homogenize the solution used a rod stirrer
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27. Making Media
2.4 Added sucrose solution, measure the pH of the solution
and added NaOH so that the pH of the solution reached 5.7
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28. Making Media
2.5 Considered agar as 0.7 g for each bottle of medium
(left), boil the solution to boiling materials for media
(middle) and media poured into the bottle (right)
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29. Making Media
2.6 Hormone cytokinin and auxin was added in the media
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30. Making Media
2.7 Media was covered by plastic and then they were
autoclaved for 20 minutes at 1210C
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31. 3. Inisiasi
3.1 Cutting explants and sterilization inside
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32. Inisiasi
3.2 Explants that had been discarded seeds and the tip of
preparation to put in the culture medium
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33. Inisiasi
3.3 Explants were placed in the medium and stored in
sterile storage area
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