This document summarizes a study that compared the effects of daily administration of garlic and onion extracts on alloxan-induced diabetic rats. Diabetes was induced in female rats by injecting alloxan monohydrate. The diabetic rats were divided into groups that received either garlic extract, onion extract, or saline by oral administration for six weeks. Blood samples were then analyzed for glucose and other biomarkers. The results showed that both garlic and onion extracts significantly reduced serum glucose levels compared to the untreated diabetic rats, with the garlic extract proving more potent. No significant differences were found between the extracts in terms of protecting the kidneys and liver.
Diabetes mellitus as reported by is a complex disorder that affects metabolism in humans and other subjects, affecting several organs in the system. Management of diabetes is challenging and its treatments are often associated with side effects as scientist is increasingly demanding for natural products with antidiabetic activity and fewer side effects [1,2]. In this study, the effect of aqueous Ipomoea batatas leaf extract was carried out in streptozotocin- induced diabetic rats (55 mg/ kg) body wt. for 28days. The continuous administration of extract from 400 mg/ kg body wt. for 28days signifi cantly (P ˂ 0.05) reversed the effects on blood glucose which was initially increased. There was a signifi cant decrease (P ˂ 0.05) in urea, creatinine, and uric which show kidney impairment following induction of the diabetogen. There was signifi cant increase (P ˂ 0.05) in the PCV, Hb, RBC, Neutrophils, Basophils, and Monocytes. While Wbc, platelets, lymphocytes, and Eosinophils were signifi cantly (P ˂ 0.05) decreased. The study therefore reveals that aqueous Ipomoea batatas leaf extract can be used in hyperglycemia management as well as in improvement in kidney and blood hematological functions.
The International Journal of Engineering and Science (The IJES)theijes
The International Journal of Engineering & Science is aimed at providing a platform for researchers, engineers, scientists, or educators to publish their original research results, to exchange new ideas, to disseminate information in innovative designs, engineering experiences and technological skills. It is also the Journal's objective to promote engineering and technology education. All papers submitted to the Journal will be blind peer-reviewed. Only original articles will be published.
Protective effects of commelina benghalensis linn (root) extract on ethanol i...IJSIT Editor
The present study was undertaken to investigate the protective effect and possible mechanism of
alcoholic (AlE) and aqueous extract (AqE) from Commelina benghalensis root (CB) on EtOH-induced hepatic
injury in Wistar rat. Hepatotoxic parameters studied in vivo include serum transaminases (AST, and ALT),
ALP, bilirubin, protein, lipid profile (Cholesterol, triglyceride, VLDL and HDL) and level of antioxidants
together with histopathological examination. Liv 52® was used as a reference hepatoprotective agent
(5ml/kg-1b.w.). AlE and AqE (200 mg/kg-1b.w.) on oral administration decreased the level of AST, ALP, ALT,
bilirubin, cholesterol, triglyceride, VLDL, MDA and increased the level of protein, HDL and antioxidants (SOD,
GSH and CAT) in rats being treated with ethanol (EtOH). Pentobarbitone -induced sleeping time study was
carried out to verify the effect on microsomal enzymes Histopathological observations confirmed the
beneficial roles of MF against EtOH-induced liver injury in rats. Possible mechanism may involve their
antioxidant activity
Effect of Aqueous Administration of White Grub and Waste Extract on the Level...BRNSS Publication Hub
Introduction: The liver plays a major role in the regulation of carbohydrate metabolism, as it uses glucose as a fuel and kidneys are to excrete metabolic waste products as well as to maintain water, pH, electrolyte balance, production of calcitriol, and hemopoietin. Aim: This study aims to investigate the effect of the administration of white grub and waste on liver and kidney indices on diabetic rats. Materials and Methods: The rats were induced with diabetes by alloxanization and treated with the extracts of white grub and waste for 2 weeks. A total of 25 rats used, were randomly distributed into five groups (G1-G5) each with five rats. G1 served as normal control. G2-G5 served as diabetic control. At the end of the 1st week of extract administration, two animals from each group were randomly selected and sacrificed. At the end of the 2nd week, the remaining three animals from each group were also sacrificed and serum was collected for the determination of liver function indices (serum alkaline phosphatase [ALP], alanine aminotransferase [ALT], aspartate aminotransferase [AST] total bilirubin [TB], direct bilirubin [DB], total protein [TP], albumin [ALB], and globulin [GLB]) and kidney function parameters (urea, creatinine, and electrolyte [sodium “Na,” potassium “K,” bicarbonate “HCO3,” and chloride “Cl”]). Results: After the 1st week, the extract-treated group (G4 and G5) showed significant reductions of ALP, ALT, AST, TP, GLB, and ALB while TB and DB have normal value compared to diabetic untreated group and for renal function (G4 and G5) showed significantly lower levels of urea, Na, K, HCO3, creatinine, and Cl. After the 2nd week, the extract-treated group showed significant reductions of ALP, ALT, AST, DB, TP, ALB, and TB with significant increased levels of GLB and TP compared to diabetic untreated group (G2). G4 (extract treated) showed significantly (P < 0.05) lower levels of urea, Na, Cl, HCO3, and creatinine and with significant increased K levels compared to G2. G5 also extract-treated group indicates significant lower levels of urea, Cl, Na, and HCO3 and higher levels of creatinine and K compared to G2. Conclusion: These results suggest that the administration of aqueous extract of white grub and waste did not have any adverse effect on the liver and kidney functions in diabetic rats. The extracts have positive effect which showed that G4 (treated with whole white grub [WG]) is more effective compared to G5 (treated with WG waste).
Antihyperglycemic and Anti-hyperlipidemic Effect of Herbamed, A Herbal Formul...CrimsonPublishersIOD
Diabetes mellitus is a metabolic disorder characterized by hyperglycemia and its occurrence is increasing fast in most of the countries. Herbal medicine derived from plant extracts have been utilized increasingly for the treatment of various disorders like diabetes mellitus. The present study was designed to evaluate the anti diabetic activity of ‘Herbamed’, a herbal formulation composed of Vernonia amygdalina, Ocimum gratissimum, Zingiber officinale and Allium sativum in alloxan-induced diabetic rats model.
Diabetes mellitus as reported by is a complex disorder that affects metabolism in humans and other subjects, affecting several organs in the system. Management of diabetes is challenging and its treatments are often associated with side effects as scientist is increasingly demanding for natural products with antidiabetic activity and fewer side effects [1,2]. In this study, the effect of aqueous Ipomoea batatas leaf extract was carried out in streptozotocin- induced diabetic rats (55 mg/ kg) body wt. for 28days. The continuous administration of extract from 400 mg/ kg body wt. for 28days signifi cantly (P ˂ 0.05) reversed the effects on blood glucose which was initially increased. There was a signifi cant decrease (P ˂ 0.05) in urea, creatinine, and uric which show kidney impairment following induction of the diabetogen. There was signifi cant increase (P ˂ 0.05) in the PCV, Hb, RBC, Neutrophils, Basophils, and Monocytes. While Wbc, platelets, lymphocytes, and Eosinophils were signifi cantly (P ˂ 0.05) decreased. The study therefore reveals that aqueous Ipomoea batatas leaf extract can be used in hyperglycemia management as well as in improvement in kidney and blood hematological functions.
The International Journal of Engineering and Science (The IJES)theijes
The International Journal of Engineering & Science is aimed at providing a platform for researchers, engineers, scientists, or educators to publish their original research results, to exchange new ideas, to disseminate information in innovative designs, engineering experiences and technological skills. It is also the Journal's objective to promote engineering and technology education. All papers submitted to the Journal will be blind peer-reviewed. Only original articles will be published.
Protective effects of commelina benghalensis linn (root) extract on ethanol i...IJSIT Editor
The present study was undertaken to investigate the protective effect and possible mechanism of
alcoholic (AlE) and aqueous extract (AqE) from Commelina benghalensis root (CB) on EtOH-induced hepatic
injury in Wistar rat. Hepatotoxic parameters studied in vivo include serum transaminases (AST, and ALT),
ALP, bilirubin, protein, lipid profile (Cholesterol, triglyceride, VLDL and HDL) and level of antioxidants
together with histopathological examination. Liv 52® was used as a reference hepatoprotective agent
(5ml/kg-1b.w.). AlE and AqE (200 mg/kg-1b.w.) on oral administration decreased the level of AST, ALP, ALT,
bilirubin, cholesterol, triglyceride, VLDL, MDA and increased the level of protein, HDL and antioxidants (SOD,
GSH and CAT) in rats being treated with ethanol (EtOH). Pentobarbitone -induced sleeping time study was
carried out to verify the effect on microsomal enzymes Histopathological observations confirmed the
beneficial roles of MF against EtOH-induced liver injury in rats. Possible mechanism may involve their
antioxidant activity
Effect of Aqueous Administration of White Grub and Waste Extract on the Level...BRNSS Publication Hub
Introduction: The liver plays a major role in the regulation of carbohydrate metabolism, as it uses glucose as a fuel and kidneys are to excrete metabolic waste products as well as to maintain water, pH, electrolyte balance, production of calcitriol, and hemopoietin. Aim: This study aims to investigate the effect of the administration of white grub and waste on liver and kidney indices on diabetic rats. Materials and Methods: The rats were induced with diabetes by alloxanization and treated with the extracts of white grub and waste for 2 weeks. A total of 25 rats used, were randomly distributed into five groups (G1-G5) each with five rats. G1 served as normal control. G2-G5 served as diabetic control. At the end of the 1st week of extract administration, two animals from each group were randomly selected and sacrificed. At the end of the 2nd week, the remaining three animals from each group were also sacrificed and serum was collected for the determination of liver function indices (serum alkaline phosphatase [ALP], alanine aminotransferase [ALT], aspartate aminotransferase [AST] total bilirubin [TB], direct bilirubin [DB], total protein [TP], albumin [ALB], and globulin [GLB]) and kidney function parameters (urea, creatinine, and electrolyte [sodium “Na,” potassium “K,” bicarbonate “HCO3,” and chloride “Cl”]). Results: After the 1st week, the extract-treated group (G4 and G5) showed significant reductions of ALP, ALT, AST, TP, GLB, and ALB while TB and DB have normal value compared to diabetic untreated group and for renal function (G4 and G5) showed significantly lower levels of urea, Na, K, HCO3, creatinine, and Cl. After the 2nd week, the extract-treated group showed significant reductions of ALP, ALT, AST, DB, TP, ALB, and TB with significant increased levels of GLB and TP compared to diabetic untreated group (G2). G4 (extract treated) showed significantly (P < 0.05) lower levels of urea, Na, Cl, HCO3, and creatinine and with significant increased K levels compared to G2. G5 also extract-treated group indicates significant lower levels of urea, Cl, Na, and HCO3 and higher levels of creatinine and K compared to G2. Conclusion: These results suggest that the administration of aqueous extract of white grub and waste did not have any adverse effect on the liver and kidney functions in diabetic rats. The extracts have positive effect which showed that G4 (treated with whole white grub [WG]) is more effective compared to G5 (treated with WG waste).
Antihyperglycemic and Anti-hyperlipidemic Effect of Herbamed, A Herbal Formul...CrimsonPublishersIOD
Diabetes mellitus is a metabolic disorder characterized by hyperglycemia and its occurrence is increasing fast in most of the countries. Herbal medicine derived from plant extracts have been utilized increasingly for the treatment of various disorders like diabetes mellitus. The present study was designed to evaluate the anti diabetic activity of ‘Herbamed’, a herbal formulation composed of Vernonia amygdalina, Ocimum gratissimum, Zingiber officinale and Allium sativum in alloxan-induced diabetic rats model.
Phytochemical evaluation and pharmacological screening of Scrophularia hyperc...SriramNagarajan18
Phytochemical evaluation and pharmacological screening of Scrophularia hypercifolia for hepatoprotective, nephroprotective and antihyperglycemic activity in alloxan induced diabetic rats Mehnoor Farheen*, Tayyaba Siddiqua
Changes in Antioxidant Enzymes in Metabolic Syndrome Patients after Consumpti...science journals
Increased oxidative stress has been suggested as an early event in the development of the metabolic syndrome and, as such, might contribute to disease progression.
Effect of crude oil pollution on phytochemical and anti diabetic potentials o...ijtsrd
Objectives This study is aimed at investigating the effect of crude oil pollution on the phytochemical content and anti diabetic potentials of Oxytenanthera abyssinica rhizomes in normal and alloxan induced diabetic rats using standard methods. Method The ability of the extracts to reduce blood glucose level in normal rats was done using 200 and 400 mg kg b.w of non crude oil polluted Oxytenanthera abyssinica extract NCOPOAE and 200 and 400 mg kg b.w of crude oil polluted Oxytenanthera abyssinica extract COPOAE respectively. Alloxan monohydrate at 130 mg kg b.w was used to induce diabetes to rats and was treated with 100, 200 and 400 mg kg b.w of NCOPOAE and 100, 200 and 400 mg kg b.w of COPOAE respectively while phytochemical analysis was done following standard method. Results Oral glucose tolerance test revealed significant decrease in plasma glucose concentration p 0.05 in groups of rats fed 200 and 400 mg kg b.w of NCOPOAE at intervals of 30, 60 and 120 minutes while that of COPOAE, none significantly decreased the plasma glucose concentration, when compared with those of control groups. The extracts did not have any significant effect in alloxan induced diabetic model. Conclusion These results could indicate that crude oil pollution could affect the synthesis of phytochemicals in plants therefore affecting the ethnomedicinal potencies. It also shows that O. abyssinca rhizomes could be more potent in management of type 2 diabetes where the cells are resistant to insulin. Kizito Ifeanyi Amaefule | Jonathan Chinenye Ifemeje | Chinenye Azuka Mere "Effect of crude oil pollution on phytochemical and anti-diabetic potentials of Oxytenanthera abyssinica (rhizomes) in normal and alloxan induced diabetic rats" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-5 , August 2019, URL: https://www.ijtsrd.com/papers/ijtsrd26557.pdfPaper URL: https://www.ijtsrd.com/biological-science/biochemistry/26557/effect-of-crude-oil-pollution-on-phytochemical-and-anti-diabetic-potentials-of-oxytenanthera-abyssinica-rhizomes-in-normal-and-alloxan-induced-diabetic-rats/kizito-ifeanyi-amaefule
Hepatoprotective Activity of Methanolic Extract of Whole Plant of Pulicaria W...IOSRJPBS
Natural remedies from medicinal plants are considered to be effective and safe alternative treatment for liver injury. The present study was conducted to evaluate the hepatoprotective activity of methanolic extract of whole plant of Pulicaria wightiana in wistar rats. The studies were conducted using the two popular inducing agents Paracetamol (2 g/kg, p.o.) in 1% NaCMC and Carbon tetrachloride (1 ml/kg). Silymarin (100 mg/kg, p.o.) was used as reference drug in the respective models. The effect was estimated by measuring the enzymatic levels and histo- pathological studies. The methanolic extract of whole plant of Pulicaria wightiana has shown very significant hepatoprotection against both Paracetamol and CCl4 - induced hepatotoxicity study models in wistar rats. This was evidenced by marked reduction in marker enzymes in serum. Histopathological studies also confirmed the hepatoprotective nature of the extract
Effect of aqueous extract of phaseolus vulgaris l. (red kidney beans) on allo...IJSIT Editor
Aqueous extract of Phaseolus Vulgaris L. (Red Kidney Beans) was investigated for its effects in
alloxan induced-diabetic rats. Twenty four albino rats were randomly allocated into four groups (A-D) of six
rats each such that group A (diabetes control) received 0.5 mL distilled water, group B (diabetes) received
400 mg/kg bwt of extract, group C (normal control) received 0.5 mL of distilled water while group D (normal)
received 400 mg/kg bwt of extract, all extract were orally administered once daily for 14 days. Diabetes was
induced in groups A&B by single interperitonial injection of 150 mg/kg alloxan monohydrate. Phytochemical
screening indicated the presence of alkaloids, balsam, flavonoids, saponins, tannins, cyanogenic glycosides,
terpenes and steroids. The hypoglyceamic potential of Phasoelus vulgaris L. was expressed in diabetes
treated rats. Blood glucose, total protein, albumin and cholesterol levels of the diabetes treated rats and
normal treated rats were not significantly (p>0.05) altered when compared with the control rats. However,
these values were significantly (p<0.05)><0.05)>0.05) effects on PCV and Hb in all groups when compared to the normal control. The study showed that
the aqueous extract of Phasoelus vulgaris L. leaves possess hypoglycaemic, antidiabetic properties and
ameliorating the high levels of marker enzymes observed in diabetes untreated rats.
Combination of Angkak (Red Yeast Rice), Red Guava (Psidium guajava Linn) Leaf...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
Antidiabetic and Cytoprotective Effect of Ethanolic Extract of SalaciaNitida ...IOSRJPBS
Objective:Many of the available therapies for diabetes have a number of serious adverse effects; therefore the search for more effective and safer hypoglycaemic agents becomes a paramount. This research was set to investigate the antidiabetic potential and cytoprotective effect of ethanolic root extract of Salacianitida on alloxan induced hyperglyceamic rats. Method:Acute toxicity and phytochemical constituents were evaluated using standardized methods. The study lasted for a period of thirty days comprising of two phases: induction phase and treatment phase. Thirty animals were grouped into five groups of six rats each. Group 1 and 2 serve as normal and toxic control respectively while groups 3, 4, and 5 were treated with 750, 1500mg of S.nitidaand 5mg glibenclamide respectively. The BGL, food and fluid intake was monitored daily while the body weight was measured on weekly. The last day of the study after an overnight fast, the animals were loaded with glucose and the OGTT measured prior to and at 30minutes interval for two hours after which the animals were sacrificed under ether anaesthesia and the organs isolated for histological examination. Results/Conclusion:The results of the present study indicate that S.nitida possessantidiabetic potentials and cytoprotective effect noted in its significant percentage reduction in the glucose level, slight increase in body weight and restoration of the toxic organs to normal.
Comparative Studies of the Aqueous Extracts of OcimumGratissimum, Aloe Vera, ...IOSR Journals
The current orthodox treatment modalities for diabetic mellitus have many setbacks including undesirable side effects and the high cost of long term treatment. The aim of this study was to determine the anti-diabetic effectsand compare some biochemical parameters of four plants; Ocimumgratissimum (OG),Aloe vera (AV), Brassica oleracea(BO) and Ipomoea batatas(IB) on alloxan induced diabetic rats. The preliminary phytochemical screening reveals the presence ofalkaloid, tannin, cardiac glycoside, resin and steroids in all four plants. Diabetic was induced by interperitonial injection of alloxan monohydrate while treatment was done for 21 days. Diabetic rats had significant increased (P˂0.05)cholesterol, triglyceride (TG), Low Density Lipoprotein (LDL) and artherogenic index (AI). Treatment of experimental rats with 150 mg/kg bw. of aqueous extractsof plants significantly decrease (P˂0.05) the levels of cholesterol and LDL in OG and BO treated groups when compare with the control. Computed artherogenic index was significantly reduced (P˂0.05) in AV treated group when compared to the control. Aqueous extracts also significantly (P˂0.05) alter serum concentrations of alkaline phosphatase (ALP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in a similar manner with metformin treated group. Result showed that decrease in plasma glucose was in the order IB˃OG˃BO˃AV. All plant extracts possess antidiabetic, anti-hyperlipidemic effects and were able to ameliorate the weight loss observed in diabetic rats
Hepatoprotective Effect of Aqueous Extracts of Some Medicinal Plant Mixtures ...IOSRJPBS
The rhizomes of Ginger (Zingiberofficinale), Turmeric (Curcuma longa), Licorice (Glycyrrhizaglabra), the bark of Cinnamon tree,(Cinnamomumzeylanicum) and the calyces of red Roselle (Hibiscus sabdariffa L.)are herbs used in thishepatoprotective studies. This study evaluates the hepatoprotective activity of water extract mixtures using carbon tetrachloride (CCl4)-induced liver injury in rats.In vitroantioxidant activity of plant water extracts was determined using DPPH. The water extractmixtures wereadministered for 10 days; on the 10thday all rats were challenged with CCl4 except control group animals. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), and albumin levels were determined to prove the hepatoprotectiveeffect.The enzyme activities were significantly increased in CCl4 treated rats. The four water extract mixtures exhibited significant (P<0.05)><0.05) increased in all the water extract mixtures used.
Phytochemical evaluation and pharmacological screening of Scrophularia hyperc...SriramNagarajan18
Phytochemical evaluation and pharmacological screening of Scrophularia hypercifolia for hepatoprotective, nephroprotective and antihyperglycemic activity in alloxan induced diabetic rats Mehnoor Farheen*, Tayyaba Siddiqua
Changes in Antioxidant Enzymes in Metabolic Syndrome Patients after Consumpti...science journals
Increased oxidative stress has been suggested as an early event in the development of the metabolic syndrome and, as such, might contribute to disease progression.
Effect of crude oil pollution on phytochemical and anti diabetic potentials o...ijtsrd
Objectives This study is aimed at investigating the effect of crude oil pollution on the phytochemical content and anti diabetic potentials of Oxytenanthera abyssinica rhizomes in normal and alloxan induced diabetic rats using standard methods. Method The ability of the extracts to reduce blood glucose level in normal rats was done using 200 and 400 mg kg b.w of non crude oil polluted Oxytenanthera abyssinica extract NCOPOAE and 200 and 400 mg kg b.w of crude oil polluted Oxytenanthera abyssinica extract COPOAE respectively. Alloxan monohydrate at 130 mg kg b.w was used to induce diabetes to rats and was treated with 100, 200 and 400 mg kg b.w of NCOPOAE and 100, 200 and 400 mg kg b.w of COPOAE respectively while phytochemical analysis was done following standard method. Results Oral glucose tolerance test revealed significant decrease in plasma glucose concentration p 0.05 in groups of rats fed 200 and 400 mg kg b.w of NCOPOAE at intervals of 30, 60 and 120 minutes while that of COPOAE, none significantly decreased the plasma glucose concentration, when compared with those of control groups. The extracts did not have any significant effect in alloxan induced diabetic model. Conclusion These results could indicate that crude oil pollution could affect the synthesis of phytochemicals in plants therefore affecting the ethnomedicinal potencies. It also shows that O. abyssinca rhizomes could be more potent in management of type 2 diabetes where the cells are resistant to insulin. Kizito Ifeanyi Amaefule | Jonathan Chinenye Ifemeje | Chinenye Azuka Mere "Effect of crude oil pollution on phytochemical and anti-diabetic potentials of Oxytenanthera abyssinica (rhizomes) in normal and alloxan induced diabetic rats" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-5 , August 2019, URL: https://www.ijtsrd.com/papers/ijtsrd26557.pdfPaper URL: https://www.ijtsrd.com/biological-science/biochemistry/26557/effect-of-crude-oil-pollution-on-phytochemical-and-anti-diabetic-potentials-of-oxytenanthera-abyssinica-rhizomes-in-normal-and-alloxan-induced-diabetic-rats/kizito-ifeanyi-amaefule
Hepatoprotective Activity of Methanolic Extract of Whole Plant of Pulicaria W...IOSRJPBS
Natural remedies from medicinal plants are considered to be effective and safe alternative treatment for liver injury. The present study was conducted to evaluate the hepatoprotective activity of methanolic extract of whole plant of Pulicaria wightiana in wistar rats. The studies were conducted using the two popular inducing agents Paracetamol (2 g/kg, p.o.) in 1% NaCMC and Carbon tetrachloride (1 ml/kg). Silymarin (100 mg/kg, p.o.) was used as reference drug in the respective models. The effect was estimated by measuring the enzymatic levels and histo- pathological studies. The methanolic extract of whole plant of Pulicaria wightiana has shown very significant hepatoprotection against both Paracetamol and CCl4 - induced hepatotoxicity study models in wistar rats. This was evidenced by marked reduction in marker enzymes in serum. Histopathological studies also confirmed the hepatoprotective nature of the extract
Effect of aqueous extract of phaseolus vulgaris l. (red kidney beans) on allo...IJSIT Editor
Aqueous extract of Phaseolus Vulgaris L. (Red Kidney Beans) was investigated for its effects in
alloxan induced-diabetic rats. Twenty four albino rats were randomly allocated into four groups (A-D) of six
rats each such that group A (diabetes control) received 0.5 mL distilled water, group B (diabetes) received
400 mg/kg bwt of extract, group C (normal control) received 0.5 mL of distilled water while group D (normal)
received 400 mg/kg bwt of extract, all extract were orally administered once daily for 14 days. Diabetes was
induced in groups A&B by single interperitonial injection of 150 mg/kg alloxan monohydrate. Phytochemical
screening indicated the presence of alkaloids, balsam, flavonoids, saponins, tannins, cyanogenic glycosides,
terpenes and steroids. The hypoglyceamic potential of Phasoelus vulgaris L. was expressed in diabetes
treated rats. Blood glucose, total protein, albumin and cholesterol levels of the diabetes treated rats and
normal treated rats were not significantly (p>0.05) altered when compared with the control rats. However,
these values were significantly (p<0.05)><0.05)>0.05) effects on PCV and Hb in all groups when compared to the normal control. The study showed that
the aqueous extract of Phasoelus vulgaris L. leaves possess hypoglycaemic, antidiabetic properties and
ameliorating the high levels of marker enzymes observed in diabetes untreated rats.
Combination of Angkak (Red Yeast Rice), Red Guava (Psidium guajava Linn) Leaf...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
Antidiabetic and Cytoprotective Effect of Ethanolic Extract of SalaciaNitida ...IOSRJPBS
Objective:Many of the available therapies for diabetes have a number of serious adverse effects; therefore the search for more effective and safer hypoglycaemic agents becomes a paramount. This research was set to investigate the antidiabetic potential and cytoprotective effect of ethanolic root extract of Salacianitida on alloxan induced hyperglyceamic rats. Method:Acute toxicity and phytochemical constituents were evaluated using standardized methods. The study lasted for a period of thirty days comprising of two phases: induction phase and treatment phase. Thirty animals were grouped into five groups of six rats each. Group 1 and 2 serve as normal and toxic control respectively while groups 3, 4, and 5 were treated with 750, 1500mg of S.nitidaand 5mg glibenclamide respectively. The BGL, food and fluid intake was monitored daily while the body weight was measured on weekly. The last day of the study after an overnight fast, the animals were loaded with glucose and the OGTT measured prior to and at 30minutes interval for two hours after which the animals were sacrificed under ether anaesthesia and the organs isolated for histological examination. Results/Conclusion:The results of the present study indicate that S.nitida possessantidiabetic potentials and cytoprotective effect noted in its significant percentage reduction in the glucose level, slight increase in body weight and restoration of the toxic organs to normal.
Comparative Studies of the Aqueous Extracts of OcimumGratissimum, Aloe Vera, ...IOSR Journals
The current orthodox treatment modalities for diabetic mellitus have many setbacks including undesirable side effects and the high cost of long term treatment. The aim of this study was to determine the anti-diabetic effectsand compare some biochemical parameters of four plants; Ocimumgratissimum (OG),Aloe vera (AV), Brassica oleracea(BO) and Ipomoea batatas(IB) on alloxan induced diabetic rats. The preliminary phytochemical screening reveals the presence ofalkaloid, tannin, cardiac glycoside, resin and steroids in all four plants. Diabetic was induced by interperitonial injection of alloxan monohydrate while treatment was done for 21 days. Diabetic rats had significant increased (P˂0.05)cholesterol, triglyceride (TG), Low Density Lipoprotein (LDL) and artherogenic index (AI). Treatment of experimental rats with 150 mg/kg bw. of aqueous extractsof plants significantly decrease (P˂0.05) the levels of cholesterol and LDL in OG and BO treated groups when compare with the control. Computed artherogenic index was significantly reduced (P˂0.05) in AV treated group when compared to the control. Aqueous extracts also significantly (P˂0.05) alter serum concentrations of alkaline phosphatase (ALP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in a similar manner with metformin treated group. Result showed that decrease in plasma glucose was in the order IB˃OG˃BO˃AV. All plant extracts possess antidiabetic, anti-hyperlipidemic effects and were able to ameliorate the weight loss observed in diabetic rats
Hepatoprotective Effect of Aqueous Extracts of Some Medicinal Plant Mixtures ...IOSRJPBS
The rhizomes of Ginger (Zingiberofficinale), Turmeric (Curcuma longa), Licorice (Glycyrrhizaglabra), the bark of Cinnamon tree,(Cinnamomumzeylanicum) and the calyces of red Roselle (Hibiscus sabdariffa L.)are herbs used in thishepatoprotective studies. This study evaluates the hepatoprotective activity of water extract mixtures using carbon tetrachloride (CCl4)-induced liver injury in rats.In vitroantioxidant activity of plant water extracts was determined using DPPH. The water extractmixtures wereadministered for 10 days; on the 10thday all rats were challenged with CCl4 except control group animals. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), and albumin levels were determined to prove the hepatoprotectiveeffect.The enzyme activities were significantly increased in CCl4 treated rats. The four water extract mixtures exhibited significant (P<0.05)><0.05) increased in all the water extract mixtures used.
Protective Effects of Ethanol Leaf-Extract of Cajanus Cajan (Linn) in Alcohol...CrimsonpublishersTTEH
Protective Effects of Ethanol Leaf-Extract of Cajanus Cajan (Linn) in Alcohol Induced Nephrotoxicity in Albino Rats by NN Ezeani* in Crimson Publishers: Telemedicine and e-Health
The protective effects of ethanol leaf-extract of Cajanus cajan in alcohol induced nephrotoxicity in albino rats were investigated. Thirty-six albino rats were assigned into six (6) experimental groups (A, B, C, D, E, F) with six rats in each group. Group A (Normal control) was administered normal saline only. Group B (standard control) was administered 5mg/kg body weight of standard drug (Silymarin); group C (positive control) was administered 3.7g/kg body weight of 99.7% ethanol while rats in groups D,E and F (test groups) were administered graded doses of 200mg/kg, 400mg/kg and 600mg/kg body weights of ethanol leaf-extract of Cajanus cajan respectively. Rats in standard control and test groups were administered 3.7g/kg body weight of 99.7% ethanol, once a day, 3 hours after administration of standard drug and ethanol leaf-extract of Cajanus cajan respectively. The administration lasted for 14 days through oral intubation. The result of our investigation showed that creatinine, uric acid and urea were significantly (P<0.05) increased in positive control group compared to the levels observed in the normal and standard control groups. However, groups administered ethanol leaf-extract of Cajanus cajan at the doses of 200mg/kg, 400mg/kg and 600mg/kg body weight all showed a significant (P<0.05) reversal in the trends of these parameters to concentrations comparable to those observed in the positive and normal control groups. The findings of this study suggest that ethanol leaf-extract of Cajanus cajan may have potentials that could be exploited for development of chemotherapies against renal toxicity.
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The Effects of Rauwolfia Vomitoria Extract on the Liver Enzymes of Carbon Tet...IOSR Journals
Rauwolfia vomitoria is a natural medicinal plant which has been used over the years for the treatment of various ailments. The effects of extract of rauwolfia vomitoria on liver enzymes of carbon tetrachloride induced hepatotoxicity were observed in adult wistar rats weighing between 120g and 190g. They were divided into four groups A,B, C and D of six rats each. Group A served as the control and received 0.41ml of distilled water. The experimental groups B, C and D received different doses of drugs as follows : group B received 0.50ml of rauwolfia vomitoria extract, group C received 0.5ml of carbon tetrachloride and group D received 0.41ml of carbon tetrachloride + 0.4ml of rauwolfia vomitoria extract. The drugs were administered once in a day using intubation method for a period of twenty one days. Twenty four hours after the last administration, the animals were anaesthetized under chloroform vapour and dissected . liver tissues were removed and weighed. Blood samples were collected by cardiac puncture and Serum samples were separated from clot by centrifugation using bench top centrifuge. Activities of serum aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase were determined using randox kit method. The relative liver weight for carbon tetrachloride treated group were significantly higher (p<0.001)><0.001) than the control. The extract exhibited a liver protective effect against carbon tetrachloride induced hepatotoxicity
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International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
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The cost of administering modern anti diabetic drugs is usually beyond the reach of most people in
the low income group especially those in the developing world where the disease is on the increase. This has led
to the current shift to the use of preparations from various parts of plants because of the current increase in the
knowledge of their toxicity, side effects, active constituents and doses. This study was conducted to compare the
effects of the aqueous Carica papaya seed and leaf extracts on serum biochemistry of alloxan induced diabetic
rats especially glucose level. Male Wister rats weighing 150-200g were induced with single freshly prepared
alloxan monohydrate (150 mg/kg body weight).Diabetes was confirmed after seven days in alloxan-induced rats
showing fasting blood glucose levels ≥ 200mg/dl. The diabetic rats were randomly allocated into three
experimental groups which received Seed extract, leaf extract or normal saline depending on the group. The
extracts were administered orally for twenty-eight days after which the animals were sacrificed and blood
samples were collected for Biochemical analyses. The results showed that both extracts have significant
hypoglycaemic, hepatoprotective and nephroprotective effects although extract of the seed proved to be more
potent than that of the leaf
Comparative Study of The Antioxidant Activities of Monodora Myristica And A. ...iosrjce
IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB) covers studies of the chemical processes in living organisms, structure and function of cellular components such as proteins, carbohydrates, lipids, nucleic acids and other biomolecules, chemical properties of important biological molecules, like proteins, in particular the chemistry of enzyme-catalyzed reactions, genetic code (DNA, RNA), protein synthesis, cell membrane transport, and signal transduction. IOSR-JBB is privileged to focus on a wide range of biotechnology as well as high quality articles on genetic engineering, cell and tissue culture technologies, genetics, microbiology, molecular biology, biochemistry, embryology, cell biology, chemical engineering, bioprocess engineering, information technology, biorobotics.
Effect of Administration Ethanol Leaf Extract of Terminalia Chebula on Liver ...oyepata
Effect of Administration Ethanol Leaf Extract of
Terminalia Chebula on Liver of Wister Rat
Joseph Oyepata Simeon1*, Modupe Builders2, Wazis Chama Haruna3, Joseph Opeyemi Tosin4, Sabastine Aliyu
Zubairu5, Musa Tabitha Lubo6, Moh’d A. Sadiq
Study the efficacy of Rhizophora mucornata Poir. leaves for diabetes therapy ...Open Access Research Paper
Hypoglycemic effects were investigated in the ethanol extract of leaves of Rhizophora mucornata on Long Evans rats. Gut perfusion and six segments studies were carried out to assess these activities. In the gut-perfusion study the percentage of glucose absorption in control rats vs. rats fed with 250 mg/kg extracts were observed at 5, 10, 15, 20, 25 and 30 minutes and the significant (p<0.05) absorption result was found at 15 minutes, which was 35.87 vs. 57.29. The percentage of absorption was found better with 250 mg/kg than 500 mg/kg dose level. The six-segment study was performed to assess the amount of sucrose remaining in the GIT at six different positions. The amount sucrose unabsorbed in different GIT segments showed that in control rats vs. rats fed with 500mg/kg extract at 30 minutes in mmol/l was 0.120 vs. 0.135 which were gradually abating with time dependent manner at 60, 180, and 360 minutes in mmol/l. These results suggest that ethanol extract of leaves of Rhizophora mucornata has significant dose dependant anti-diabetic effects which may be effective in the treatment of diabetes.
Evaluation of Hepatoprotective Activity and Oxidative Stress parameters of Al...gynomark
In spite of the tremendous advances made, no significant and safe
hepatoprotective agents are available in modern therapeutics.
Therefore, due importance has been given globally to develop plantbased
hepatoprotective drugs, effective against a variety of liver
disorders. The aim of the current study is to screen the alcoholic
extract of Artabotrys hexapetalus (L.f) Bhandari (AEAH) for
hepatoprotective activity in rats which were intoxicated by
paracetamol. This article describes phytochemical (qualitative),
hepatoprotective activity and oxidative stress parameters of the above
selected plant drugs by studying the serum enzyme levels like SGOT,
SGPT, ALP, ACP, Total Bilirubin, Direct Bilirubin, SOD, GSH,
Vitamin C and Catalase levels of the animals treated with hepato
toxicant paracetamol. The alcoholic extract of AEAH reversed the
hepatotoxicity induced by paracetamol in the rats, indicating their
hepato-protective action. The study was also supported by the
histopathological studies which reversed structural damage occurred
due to paracetamol. This study was further supported by the DNA
fragmentation studies which showed the absense of fragmentation of
DNA in AEAH treated groups, indicating the hepatoprotective activity
of Artabotrys hexapetalus (L.f) Bhandari. Hence it can be concluded
that the plant extract possesses a promising hepatoprotective and
antioxidant effect.
SUB-ACUTE TOXICITY STUDY OF ETHANOL LEAF EXTRACT OF Ocimum canum ON THE KIDNE...oyepata
SUB-ACUTE TOXICITY STUDY OF ETHANOL LEAF EXTRACT OF Ocimum canum ON
THE KIDNEY OF WISTAR RATS
JOSEPH OS*1, BUILDERS M1, JOSEPH OT2, SABASTINE AZ3, MUSA TL4, OYEPATA PJ2
Toxicity Studies of Aqueous-Methanol Extract of Dennettia tripetala (Pepper f...Scientific Review SR
Traditional medicine still remains the main recourse for a large majority of people for treating health problems in African. Therefore, the aim of this work is to assess the toxicological effect of the fresh ripe fruits using two solvents for extraction. The toxicological evaluation of aqueous-methanol extract of Dennettia tripetala fresh ripe fruits at 100, 200 and 400 mg/kg body weight for 14 days on some biochemical parameters in wistar rats was investigated. The extract at all the doses tested show non-significant (p > 0.05) increase from the control in ALT, AST, ALP, total protein, albumin, direct bilirubin, creatinine, Na+ and K+, while the level of total bilirubin and urea show significant (p < 0.05) increase from the control at 400mg/kg body weight. The levels of SOD, GPx, GST, and GSH in the serum were significantly (p < 0.05) decrease in the treated rats at 200 and 400mg/kg body weight, whereas the level of MDA and CAT showed non-significant (p > 0.05) increase in all the animals. The results of this finding indicated that the aqueous-methanol extract may not have serious effect on the liver and the kidney at 100 mg/kg b.d., but may be toxic at high doses as observed in the acute toxicity, sub-acute results and antioxidant parameters where it shows a dose-specific effects.
Pharmacological evaluation of hepatoprotective activity of Ethanolic extract ...pharmaindexing
Liver diseases are still a worldwide health problem due to drug-induced hepatotoxicity. It may occur as an unexpected idiosyncratic reaction, or nontoxic drug or it may be an expected consequence of the intrinsic toxicity of a drug, taken in a sufficiently large dose to cause liver injury. A highly potential therapeutic agent or a medicinal extract is necessary for the preventive action of the hepatic disorders leading to the inflammation and drug inducing liver injury. The present study proved the medicinal plant with supportive therapeutic efficacy. Albino wistar rats of either sex are induced by Rifampicin and D-Galactosamine orally at a dose of 1g/kg and 400 mg/kg for a period of 28 days, and were treated with ethanolic extract of the stems of Andrographis lineata Nees (EEALN) orally at a dose of 200 and 400 mg/kg/day. The biochemical parameters such as serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP),Total protein, and serum bilirubin (Total and Direct) were estimated to assess the liver function. Ethanolic extract of the stems of Andrographis lineata Nees (EEALN) showed the hepatoprotective activity by decreasing the levels of serum hepatic marker enzymes. Silymarin is used as a Standard drug. Histopathological studies were performed to confirm the biochemical changes in the hepatocytes. Toxicological studies were carried out with the extract and 2000 mg/kg b.wt. is considered as the safe dose with no mortality and adverse effects.
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Comparative Effect of Daily Administration of Allium sativum and Allium cepa Extracts on Alloxan Induced Diabetic Rats
1. IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB)
e-ISSN: XXXX-XXXX, p-ISSN: XXXX-XXXX, Volume 1, Issue 2 (Jan. – Feb. 2015), PP 26-31
www.iosrjournals.org
www.iosrjournals.org 26 | Page
Comparative Effect of Daily Administration of Allium sativum
and Allium cepa Extracts on Alloxan Induced Diabetic Rats
*Ojo Rotimi Johnson *Opara Paul Emeka, *Babatunde Peter Femi
1
Department of Biochemistry, Faculty of Science and Technology, Bingham University Karu Nasarawa state,
Nigeria
Abstract: Effective regulation of blood glucose level is the most important factor for reducing
risks of diabetic complications and this must be done without any side effects or interference
with normal physiological function of the body which is not usually achievable with the
conventional diabetic drugs. This has resulted in the current shift to the use of herbal
preparation which is presumed to be effective, cheap with no side effects. Most noted among
these are onion and garlic. This present study was therefore conducted to compare the effects
of daily administration of garlic and onion extracts on alloxan induced diabetic rat. Diabetes
was induced in Female Wister rats weighing 150-200g by intraperitoneal injection of freshly
prepared alloxan monohydrate (150 mg/kg body weight).Diabetes was confirmed after seven
days in rats showing fasting blood glucose levels ≥ 200mg/dl. The diabetic rats were
randomly allocated into three experimental groups which received garlic extract, onion
extract or normal saline depending on the group. The extracts were administered orally for
six weeks after which the animals were sacrificed and blood samples were collected for
Biochemical analyses. From the results obtained it can be concluded that the extracts of
fresh Allium sativum and Allium cepa significantly reduced the serum glucose with Allium
sativum extract proving to be more potent while no significant difference was shown in their
nephro- and hepato-protective activities.
Keyword: alloxan, diabetes, Garlic, herbal preparation, Onion
I. Introduction
The current prevalence of diabetes mellitus in the third world countries especially Africa has been
linked to the spread of Western life styles like physical inactivity combined with diets that are high in calories;
processed carbohydrates, saturated fats and insufficient dietary fiber [1, 2].The estimated number of people with
diabetes is expected to hit 380 million by 2025 and most of them will likely be found in Asia and Africa [3, 4].
Diabetes mellitus has been reported to be the major cause of blindness, kidney failure, lower-extremity
amputation, cardiovascular diseases and premature mortality [5]. Effective control of blood glucose level is still
the most important factor for reducing risks of diabetic complications [6] and this must be done without any side
effects or any interference with normal physiological function of the body which is not usually achievable with
the conventional diabetic drugs. This has resulted in the current shift to the use of herbal preparation which is
presumed to be effective, cheap with fewer or no side effects [7];most noted among these are ginger, onion and
garlic which appear to be most effective and least toxic [8].
Allium cepa belongs to the family Liliaceae and is widely cultivated throughout the world [9], its most
popular modern uses include regulation of blood pressure, blood glucose ,blood cholesterol level[9,10] and as
antiseptic[11]. Active ingredient in A. cepa include phenolic compounds (flavonoids, anthocyanins, phenolic
acids and flavonols), organosulphur compounds, vitamins and some minerals [12, 13, 14], these compounds
mediate the pharmacological effects of A. cepa. Thus, phenolic acids, such as caffeic, chlorogenic, ferulic,
sinapic, p-coumaric acids, vanillic, syringic and p-hydroxybenzoic appear to be active antioxidants [15, 16]. Its
vitamins especially vitamin C have a protective function against oxidative damage and a powerful quencher of
singlet oxygen (O2), hydroxyl (OH) and peroxyl (RO2) radicals[17,18]. Garlic bulb contains chemically active
substances such as enzymes, amino acids, minerals and sulphur containing compounds such as alliin (S-allyl
cysteine sulphoxide (SACS) and allicin (diallyl disulphide) which are responsible for garlic’s pungent odour and
many of its medicinal effects [19]. Allicin which is associated with garlic action does not exist in garlic until it
is crushed or cut where injury to the garlic bulb activates the enzyme allinase which metabolizes allin to
allicin[20].Garlic has been found to have antibacterial and antifungal activity, reduce aortic plaque deposits[21],
2. Comparative Effect of Daily Administration of Allium sativum and Allium cepa Extracts on....
www.iosrjournals.org 27 | Page
inhibits vascular calcification in human patients with high blood cholesterol[22], reduce hyperlipidemia[23 ,24]
and hyperglycaemia[25].The primary purpose of this research was to compare biochemical effects of Allium
sativum and allium cepa extracts in alloxan induced diabetic rats.
II. Materials And Methods
2.1 Collection of Plant Samples
Plant samples were obtained from Masaka market in Nasarawa State and identified in Biological
Sciences Department, Bingham University karu, Nasarawa State, Nigeria.
2.2 Preparation of Allium sativum (Garlic) and Allium cepa(onion)
The same method was used to prepare garlic and onion extract from their respective bulbs as follow:
fresh healthy bulbs of garlic or onion weighing 50g were washed and peeled; the peeled bulbs were then cut
into small pieces and homogenized in 70 ml cold, sterile 0.9% NaCl in the presence of some crushed ice. The
homogenization was carried out in a blender at high speed using 30-second bursts for a total of 10 minutes. The
homogenized mixture was filtered 3 times through cheesecloth, the filtrate was centrifuged at 2000 RCF for 10
minutes and the clear supernatant obtained was diluted to100 ml with normal saline. The concentration of this
garlic or onion preparation was considered to be 500 mg/ml on the basis of the weight of the starting material
(50g/100 ml). The aqueous extract of the garlic or onion was stored at 4 °C until use.
2.3 Experimental Animal
Female wister rats weighing 150-200g were purchased from Bingham University animal house
Nasarawa state. The rats were approved for the experiment by the local committee and were housed in
individual plastic cages with stainless steel at room temperature (25+3o
C) at 12 hr dark-light cycles. All the rats
were allowed free access to the same diets and water and acclimatized for 14 days before the treatment.
2.4 Preparation of alloxan
Two grams of crystalline alloxan monohydrates were dissolved in 50mls of normal saline (0.9% NaCl
solution) to yield a concentration of 40mg/ml.
2.5 Induction of diabetes
The rats were divided into two groups before the induction of diabetes: non-diabetic control group and
experimental group (to be induced with alloxan) with the weights of all the rats taken before alloxan
administration.
Diabetes was induced in the experimental rats after overnight fasting (12hrs) by intraperitoneal
administration of 150mg of alloxan per kilogram body weight of rat (150mg/kg body weight).
After the induction, all the rats were allowed free access to the same feed and water and the alloxan-
induced rats were carefully examined for the next 24hrs for evidence of allergic reactions, behavioural changes
and convulsion.
After one week, blood was collected from the tail vein and diabetes was confirmed in alloxan treated
rat with fasting blood glucose levels greater than 200mg/dl.
2.6 Experimental Design
The control and experiment animals were divided into different groups and treated accordingly:
Group 1 (non-diabetic group): normal control (receive 1ml normal saline through direct stomach intubation
every day).
Group 2 (Diabetic control): receive 1ml normal saline through direct stomach intubation every day.
Group 3 (Garlic group): Diabetic rats receiving 400mg garlic extract per kilogram body weight daily.
Group 4 (Onion group): Diabetic rats receiving 400mg onion extract per kilogram body weight daily.
2.7 Preparation of samples for biochemical analysis
After six weeks of treatment with the different extracts, the body weights of all the rats were taken
again and were allowed to fast for 12hr before they were sacrifice under sodium pentobarbitone anaesthesia.
Whole blood was collected via cardiac puncture using sterile syringes and needles and emptied into plain
bottles; this was allowed to clot for about two hours. The clotted blood was centrifuged at 3,500rpm for 30mins
to recover the serum from clotted blood. Serum was separated with sterile syringes and needles and stored
frozen until it is used for biochemical analysis.
3. Comparative Effect of Daily Administration of Allium sativum and Allium cepa Extracts on....
www.iosrjournals.org 28 | Page
2.8 Assays
The blood glucose in a protein free serum was determined as described by Sood [26]. Urea, creatinine
and total bilirubin concentrations were determined by the methods of Patton and Crouch [27] Henry et al.[28]
and Pearlman and Lee[29], respectively. Total cholesterol was measured by the procedure described by Allain et
al.,[30]. Serum aspartate amino transferase (AST) and alanine amino transferase (ALT) activities were estimated
with the Randox reagent kit using 2, 4-dinitrophenylhydrazine as substrate according to the method described by
Reitman and Frankel[31]. Protein content was determined by the method of Lowry et al. [32] while Alkaline
phosphatase was assessed as described by Principato et al.,[33]. All the assays were carried out at the
Department of Biochemistry, Bingham University Karu Nasarawa state, Nigeria.
III. Result And Discussion
The administration of garlic extracts to the diabetic rats significantly reduced serum glucose compared
to the diabetic group that did not receive the same treatment, although these reductions were not enough to bring
it down to the normal rats’ serum glucose levels (Table 1). This effect of garlic has been reported for both
alloxan and streptozotocin- induced diabetic rats[25,34,35,36], this ability to reduce serum glucose level is
associated with the presence of S-allyl cysteine sulphoxide, a sulphur-containing amino acid which has been
shown to posses the potential to reduce the blood glucose in rats almost to the same level as
insulin[37,38].These active constituents of garlic extract are believed to act as hypoglycaemic agent by
increasing either the pancreatic secretion of insulin from the beta cell or its release from bound insulin[2,39,40],
interferes with intestinal glucose absorption[41] and peripheral glucose utilization[42,43] and lastly by
stimulating the few surviving β-cells to produce insulin or regenerates β-cells of the islets, since β-cells have
been shown to have a potential for regeneration[41, 44,45].
Onion extract also significantly reduce the serum glucose level .This has also been reported before by
Eyo et al.[2] .This action may be attributed to allyl propyl disulphide and other sulphur compounds in
onions[2,46] which may act by mopping up the Reactive Oxygen Species (ROS) and free radicals which
mediate the toxic action of alloxan on pancreatic beta cells[47,48,49,50].The best reduction in blood glucose
level was however observed with garlic extract (70.23%) compare to onion(63.35%) while normal saline
administered to the diabetic rats did not affect the glucose levels.
Table 1: Effect of Allium cepa and Allium sativum on Blood Glucose Level of Diabetic Rats
TREATMENT DAY 1 (mg/dl) DAY 28 (mg/dl) % Change In Glucose Level
NORMAL CONTROL 77.00 + 7.35 79.00 + 6.00 + 2.60
DIABETIC CONTROL 245.50 + 4.91 338.75 + 7.42 + 37.98
ONION(400mg/kg) 231.25 + 3.40 84.75 + 1.85 -63.35
GARLIC (400mg/kg) 374.50 + 9.29 111.50 + 10.29 -70.23
All Values Are Expressed As Mean ± Standard Deviation of Four Observations
As shown in Table 2, alloxan induction produced significant increase in the hepatic marker enzymes
(ALT, AST and ALP). The increase in these hepatic marker enzymes (ALT, AST, and ALP) levels is as a
result of these enzymes leaking from liver cells cytosol into bloodstream[51] since the toxic action of alloxan is
mediated through the generation of free radicals[47,48] which eventually damage the liver cells leading to the
leakage of these enzymes which are located in the cell cytoplasm once the cellular membrane integrity is
compromised; the serum concentrations of these enzymes are usually proportional to the damage[51,52]. In
particular, the elevation of ALT an enzyme found mainly in the liver is an indication of liver damage.
Significant reduction in the activities of these enzymes in garlic and onion extracts treated diabetic rats is an
indication of their hepatoprotective activities [53, 54]. These protective effects of onion and garlic extracts may
be associated with their inherent-antioxidant properties as reported by Rahman [49] which is believe to
accelerate the regenerative capacity of the hepatocytes, cause stabilization of the cell membrane of the
hepatocytes, and ultimately protect the liver cells against deleterious agents and free radical mediated toxic
damages. For example,onion contains sulphur-containing compounds of which their oxidized thiols can trap
electrons from other systems[35,55,56,57].Generally, from the results(TABLE 2),there is no significant
difference between the hepatoprotective effects of garlic and onion in contrast to the observation of Micheal et
al.,[58] and Tatfeng and Enitan[59] who reported that garlic protects liver from damages better than onion.
Table 2: Effect of Allium cepa and Allium sativum on the Liver of the Treated Diabetic Rats
BIOCHEMICAL
PARAMETER
AST (U/I) ALT(U/I) ALP(U/I)
NORMAL CONTROL 35.50a
+ 0.58 20.85a
+ 0.98 22.26b
+ 0.64
DIABETIC CONTROL 80.00c
+ 5.77 37.65c
+ 6.73 63.81d
+ 8.90
ONION(400mg/kg) 40.50b
+ 0.58 24.00b
+ 1.15 15.40a
+ 2.53
GARLIC (400mg/kg) 40.00b
+ 0.50 23.50b
+ 1.73 26.95c
+ 0.63
4. Comparative Effect of Daily Administration of Allium sativum and Allium cepa Extracts on....
www.iosrjournals.org 29 | Page
All Values Are Expressed As Mean ± Standard Deviation of Four Observations.
Values with different superscript in the same column are significantly different at p< 0.05
Serum urea and creatinine are often regarded as reliable markers of renal function status Thus,
elevations in the serum concentrations of these markers are signs of renal injury [60].Administration of both
garlic and onion extracts led to reduction of these kidney biomarkers (Table 3).Their nephroprotective properties
have been proposed to be mediated through antioxidant or free radical scavenging activities due to the high
concentration of flavonoids and alkaloids they contain[61,62].These showed that the extract of garlic and onion
enhanced the ability of the kidneys to remove these waste products from the blood as indicated by reduction in
serum urea and creatinine levels and thus, confer protective effect on the kidney of diabetic rats[35].
Alteration in serum lipids profile is well known in diabetes [63, 64] including alloxan diabetic rats [65].
In this study, the untreated diabetic rats showed hypercholesterolemia, similar to the earlier reports of Abdul
Rahman et al. [66] and Idogun et al. [67] among diabetic patients.
Studies with raw garlic and onion have shown that they significantly reduce the total serum cholesterol
[50, 68].The hypocholestrolemic activities of garlic and onion are due to the presence of allicin and allyl propyl
disulphide respectively together with their derivatives compounds [69] . Other non-sulphur components like
saponins might also have contributed to their actions.These hypocholesterolemic effect is due to the abilities
of the active substances to limit hepatic cholesterol biosynthesis[50,70,71,72], enhance cholesterol turnover to
bile acids and its excretion through gastrointestinal tract or by inhibiting cholesterol absorption from intestinal
lumen without changing HDL-cholesterol levels[22,23,24,50].
Table 3: Serum Levels of Urea, Creatinine, and Cholesterol In The Treated Rats.
TREATMENT UREA (mg/dl) CREATININE
(mg/dl)
CHOLESTEROL (mg/dl)
NORMAL CONTROL 26.32a
+ 1.50 0.45a
+ 0.06 51.29a
+ 0.33
DIABETIC CONTROL 103.57c
+ 9.21 1.36d
+ 0.16 128.70c
+ 9.28
ONION(400mg/kg) 29.86b
+ 1.79 0.67b
+ 0.04 56.17b
+ 0.77
GARLIC (400mg/kg) 29.00b
+ 0.50 0.81c
+ 0.01 55.10b
+ 2.70
All Values Are Expressed As Mean ± Standard Deviation of Four Observations.
Values with different superscript in the same column are significantly different at p< 0.05
IV. Conclusion
From the results obtained it can be concluded that the extracts of fresh Allium sativum and Allium
cepa significantly reduced serum glucose and can also protect liver and kidney in diabetic patients although
the extract of garlic seems to be more effective in reducing the blood glucose.
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