In-vitro evaluation of Antioxidant and Anti-diabetic activity of leaf extract of Kigelia africana

1. 1
IN-VITRO EVALUATION OF ANTIOXIDANT AND ANTI-DIABETIC ACTIVITY OF
KIGELIA AFRICANA
Sreejith P S (15MSB0045), Majesh Mathew (15MSM0070).
Research Guide: Dr. Devi Rajeswari V
Assistant Professor (Sr),
School of Biosciences and Technology,
VIT University, Vellore-14.
13SETMSB1300180

2. ABSTRACT
• Aqueous leaf extract of medicinal plant Kigelia africana were screened for :
 Phytochemical analysis
 DPPH radical scavenging activity
 Total Antioxidant activity
 Hydrogen peroxide scavenging activity
 In-vitro Anti-diabetic acitivity
 Non enzymatic glycosylation hemoglobin assay
 Inhibition of alpha amylase assay
 Glucose uptake by yeast cells

3. INTRODUCTION
• Diabetes – A gathering of metabolic illnesses described by hyperglycemia coming about because
of imperfections in insulin discharge, insulin activity, or both.
Causes: Diabetic ketoacidosis, coronary illness, stroke, unending kidney disappointment, foot
ulcers and harm to eyes.
• Antioxidants are involved in prevention of cellular damage- Cancer, aging and variety of
diseases.
• Medicinal plants assume a vital part in the administration of diabetes mellitus particularly in
creating nations where assets are pitiful.
• The antioxidant activity of Kigelia africana was evaluated by measuring reducing ability, free
radical scavenging activity by DPPH and hydrogen peroxide methods.

4. 4
METHODOLOGY
Sample collection Extraction process
Phytochemical
analysis
Antioxidant activityAnti –diabetic activity

5. Sample collection
Kigelia africana leaves

6. Extraction process

7. RESULTS AND DISCUSSION:
1. Phytochemical analysis
• Qualitative analysis
S.no Phytochemical tests Qualitative analysis Results
1. Alkaloids Wagner’s test -
2. Carbohydrates Benedict,s test -
3. Proteins and
aminoacid
Millions test -
4. Glycoside Borntrager’s test ++
5. Saponins Foam test +++
6. Steroids and sterols Salkowski test ++
7. Phenolic Lead acetate test +++
8. Tannins Ferric chloride test +++
9. Flavanoids Sodium hydroxide
test
++

8. 2. Antioxidant activity:
DPPH radical scavenging activity

9. 3. Total Antioxidant activity:
4. Hydrogen peroxide scavenging activity:
0
10
20
30
40
50
60
70
25 50 75 100
Absorbance Concentration in µg/ml
Hydrogen Peroxide
Aqueous extract
Ascorbic acid
0
10
20
30
40
50
60
70
80
25 50 75 100
Absorbance
Concentration in µg/ml
Total antioxidant activity
Aqueous extract
Ascorbic acid

10. • 6. In-vitro anti diabetic activity
• Non enzymatic glycosylation hemoglobin assay
0
10
20
30
40
50
60
70
80
90
200 400 600 800 1000
%inhibition
concentration (µg/ml)
Non Enzymatic Glycosylation of Haemoglobin Assay
tocopherol
aqueous
• Inhibition of alpha amylase assay
0
10
20
30
40
50
60
70
200 400 600 800 1000
%inhibition Concentration (µg/ml)
Alpha Amylase Inhibition
acarbose
aqueous

11. • Glucose uptake by yeast cells
0
10
20
30
40
50
60
70
80
40 80 120 160 200
%ofuptake
Concentration (µg/ml)
Glucose uptake by yeast cell (10mM)
acarbose
aqueous
0
10
20
30
40
50
60
70
80
40 80 120 160 200
%ofuptake
Concentration (µg/ml)
Glucose uptake by yeast cells (5mM)
acarbose
aqueous

12. Conclusion:
As indicated by all tried method, the cell reinforcement action of Kigelia africana was contrasted and that of Ascorbic
acid, which is an actually happening natural compound with cancer prevention agent movement. All in all, the present
study reports that Kigelia africana concentrate can be a decent premise of cancer prevention agent movement and it
likewise can hinder glucose dispersion, α–amylase action and to enhance glucose take-up action by yeast cells utilizing as
a part of vitro models. The confirmation recommend that the plant might be helpful in dynamic administration of Type2
Diabetes mellitus. A great deal more examinations are required to clarify whether in-vitro impacts speak to remedial
probability in-vivo models for refining glycemic control in Type 2 Diabetes.

13. Reference
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