This document describes an experiment to study the effect of drugs on dog blood pressure. It discusses the history of measuring blood pressure, the apparatus used including a Poiseuille mercury manometer, and data on normal dog physiology. The procedure involved anesthetizing dogs, inserting cannulas, and using a Marey's tambour to measure blood pressure. Various drugs were tested including adrenaline, noradrenaline, acetylcholine, and isoprenaline and their effects on blood pressure were observed and explained.

1. DOG BLOOD PRESSURE
DR. RESU NEHA REDDY
2ND YEAR PG
MD PHARMACOLOGY
OSMANIA MEDICAL COLLEGE

2. HISTORY
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• STEPHEN HALES
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3. •
• JEAN LÉONARD MARIE
POISEUILLE
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4. • CARL LUDWIG
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5. AIM :
TO STUDY THE EFFECT OF DRUGS ON DOG
BLOOD PRESSURE

6. APPARATUS :
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7. POISEUILLE MERCURY MANOMETER

8. POISEUILLE MERCURY MANOMETER

9. ARTERIAL CANNULA VENOUS CANNULA

10. DISSECTION INSTRUMENTSTRACHEAL CANNULAS

11. DOG BOARD AND MOUTH ROD

12. PHYSIOLOGICAL DATA - DOG
• 140 / 80
• 5000 – 8000 GMS
• 37.7 °C
• 14 – 28 / MIN
• 77 – 138 / MIN
• 60

13. PROCEDURE

20. BRODIE’S / MAREY’S TAMBOUR

22. ANAESTHETICS USED :
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23. BARBITURATES
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• PHENOBARBITONE
• PENTOBARBITONE
• THIOPENTONE

24. CHLORALOSE
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• ADVANTAGES
• DISADVANTAGES
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25. URETHANE ( ETHYL CARBAMATE)
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• DISADVANTAGES
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26. PARALDEHYDE
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27. MAGNESIUM SULPHATE
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• EUTHANASIA

28. CHEMICALS / DRUGS USED
 PENTOBARBITONE
 ADRENALINE
 NOR-ADRENALINE
 ACETYLCHOLINE
 ISOPRENALINE
 ALPHA BLOCKER
 BETA BLOCKER
 EPHEDRINE
 ISOTONIC SALINE
 8.5 % SODIUM CITRATE
 1 % HEPARIN

29. ADRENALINE
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• ALPHA 1
• BETA 2
• BETA 1

30. ADRENALINE

31. NON-ADRENALINE
• RISE BP
• NO BETA 2

32. ISOPRENALINE
• BETA AGONIST
• TACHYCARDIA
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VASODILATION
• FALL IN BP
• SLOW METABOLISM

33. ALPHA BLOCKER – DALE’S VASOMOTOR REVERSAL
ALPHA BLOCKER
Epi NA Iso Epi NA Iso

34. BETA BLOCKER
BETA BLOCKER
Epi NA Iso Epi NA Iso

35. RE-REVERSAL PHENOMENON
ALPHA BLOCKER BETA BLOCKER
Epi NA Iso Epi NA Iso Epi NA Iso

36. ACETYLCHOLINE
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• ACHE
• M1 ++
• M2 - -
• M3 ++
• NO ENDOTHELIAL
• SHARP TRANSIENT FALL IN BP

37. ACETYLCHOLINE 
ANTI CHOLINESTERASE
ANTI CHOLINESTERASE

38. ACETYLCHOLINE 
Car Pilo Ach Car Pilo Ach Car Pilo Ach
ATROPINE
Nicotinic action

39. NICOTINIC ACTION OF ACETYLCHOLINE

40. EPHEDRINE
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• RELEASES NOR-ADRENALINE
• NO BETA 2
• BETA 2
• BETA 1
• SUSTAINED RISE IN BP
• TACHYPHYLAXIS

41. EPHEDRINE
Act by  releasing catecholamines
 synthesis of which does not match release
 deplete stores rapidly

42. TACHYPHYLAXIS

43. TACHYPHYLAXIS
• REDUCTION IN RESPONSIVENESS
• ACUTE TOLERANCE
•
EPHEDRINE
• SLOW DISSOCIATION
• TACHYPHYLAXIS

45. Epi NA Epi NA
GUANETHIDINE
GUANETHIDINE

46. EXAM POINT OF VIEW…

51. REFERENCES :
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52. THANK YOU..!!