A rapid culture independent methodology toquantitatively detect and identify common human bacterial pathogens associated with contaminated high purity water
A presentation about the recent developments in Rapid microbiological detection methods from LumiByte.
MuScan for instant single cell detection and the colony tracker for ultra fast antibiotic suseptibility testing.
Presented at the Health and Technology event in Papendal the netherlands september 2013
For over 10 decades, agents of infectious diseases have been identified through their phenotype directly in specimen and after a growth in culture.
Today, we are in a molecular era, there is an opportunity to detect organisms more rapidly and accurately based on their genetic signatures.
Biomedical science research discovery offers a growing numbers of a nucleic acid amplification tests (NAATS) among which is polymerase chain reaction (PCR) for detection and identification of bacterial, parasitic, fungi and viral pathogens.
These assays improve patient care, reduce antibiotic usage, enhance test utilization and increase laboratory and hospital efficiency.
In this seminar, we will explore the clinical usefulness and potential of both conventional and real-time PCR assays in Clinical Microbiology.
A presentation about the recent developments in Rapid microbiological detection methods from LumiByte.
MuScan for instant single cell detection and the colony tracker for ultra fast antibiotic suseptibility testing.
Presented at the Health and Technology event in Papendal the netherlands september 2013
For over 10 decades, agents of infectious diseases have been identified through their phenotype directly in specimen and after a growth in culture.
Today, we are in a molecular era, there is an opportunity to detect organisms more rapidly and accurately based on their genetic signatures.
Biomedical science research discovery offers a growing numbers of a nucleic acid amplification tests (NAATS) among which is polymerase chain reaction (PCR) for detection and identification of bacterial, parasitic, fungi and viral pathogens.
These assays improve patient care, reduce antibiotic usage, enhance test utilization and increase laboratory and hospital efficiency.
In this seminar, we will explore the clinical usefulness and potential of both conventional and real-time PCR assays in Clinical Microbiology.
RECENT ADVANCES IN DIAGNOSIS OF TUBERCULOSISANGAN KARMAKAR
TRADITIONAL TESTS AND RECENT DIAGNOSTIC MODALITIES FOR TUBERCULOSIS WITH EMPHASIS TO MOLECULAR DETECTION TECHNIQUES, DRUG SENSITIVITY ASSESMENT IN INDIAN PERSPECTIVE
This presentation is about lab diagnosis of tuberculosis. It highlights use of currently available diagnostic methods in identifying pulmonary and extrapulmonary tuberculosis.
Profiling Hospital-Acquired Pathogens and Antibiotic Resistance Genes WebinarQIAGEN
Hospital-acquired infections (HAIs) are caused by bacterial, viral and fungal pathogens that easily spread through the body. The most common HAIs include urinary tract infections, bloodstream infections and pneumonia. HAIs are becoming more virulent and more resistant to the antibiotics typically used to fight them, making antibiotic resistance a serious public health concern. In this webinar, we will provide an overview of hospital-acquired pathogens and antibiotic resistance. We will also present tools to help you identify and characterize hospital-acquired bacterial species and antibiotic resistance genes in your research samples.
RECENT ADVANCES IN DIAGNOSIS OF TUBERCULOSISANGAN KARMAKAR
TRADITIONAL TESTS AND RECENT DIAGNOSTIC MODALITIES FOR TUBERCULOSIS WITH EMPHASIS TO MOLECULAR DETECTION TECHNIQUES, DRUG SENSITIVITY ASSESMENT IN INDIAN PERSPECTIVE
This presentation is about lab diagnosis of tuberculosis. It highlights use of currently available diagnostic methods in identifying pulmonary and extrapulmonary tuberculosis.
Profiling Hospital-Acquired Pathogens and Antibiotic Resistance Genes WebinarQIAGEN
Hospital-acquired infections (HAIs) are caused by bacterial, viral and fungal pathogens that easily spread through the body. The most common HAIs include urinary tract infections, bloodstream infections and pneumonia. HAIs are becoming more virulent and more resistant to the antibiotics typically used to fight them, making antibiotic resistance a serious public health concern. In this webinar, we will provide an overview of hospital-acquired pathogens and antibiotic resistance. We will also present tools to help you identify and characterize hospital-acquired bacterial species and antibiotic resistance genes in your research samples.
INTRODUCTION
FACE RECOGNITION
CAPTURING OF IMAGE BY STANDARD VIDEO CAMERAS
COMPONENTS OF FACE RECOGNITION SYSTEMS
IMPLEMENTATION OF FACE RECOGNITION TECHNOLOGY
PERFORMANCE
SOFTWARE
ADVANTAGES AND DISADVANTAGES
APPLICATIONS
CONCLUSION
Viral metagenomics is the study of viral genetic material sourced directly from the environment rather than from a host or natural reservoir. The goal is to ascertain the viral diversity in the environment that is often missed in studies targeting specific potential reservoirs.
Microbiome Identification to Characterization: Pathogen Detection Webinar Ser...QIAGEN
The research community has begun correlating the makeup of individual microbiomes with disorders and diseases such as autism, atherosclerosis, obesity and cancer. To accomplish this, researchers must first identify and characterize these microbial communities. This slidedeck will begin with a general introduction of metagenomics and an overview of experimental strategies. Following this, a comprehensive microbiome assay pipeline will be introduced. We conclude with application-based examples that demonstrate how to identify and characterize microbiome profiles.
A Vaccine Approach against HIV-1, Manufacturing Env proteins: from Bench to B...KBI Biopharma
A Vaccine Approach against HIV-1, Manufacturing Env proteins: from Bench to Bedside
Abhinav A.Shukla, Ph.D. Senior Vice President, Process Development & Manufacturing, KBI Biopharma
Prof.Barton Haynes,M.D.Director,Duke Human Vaccine Institute
UNDERSTANDING WHAT GREEN WASHING IS!.pdfJulietMogola
Many companies today use green washing to lure the public into thinking they are conserving the environment but in real sense they are doing more harm. There have been such several cases from very big companies here in Kenya and also globally. This ranges from various sectors from manufacturing and goes to consumer products. Educating people on greenwashing will enable people to make better choices based on their analysis and not on what they see on marketing sites.
WRI’s brand new “Food Service Playbook for Promoting Sustainable Food Choices” gives food service operators the very latest strategies for creating dining environments that empower consumers to choose sustainable, plant-rich dishes. This research builds off our first guide for food service, now with industry experience and insights from nearly 350 academic trials.
Artificial Reefs by Kuddle Life Foundation - May 2024punit537210
Situated in Pondicherry, India, Kuddle Life Foundation is a charitable, non-profit and non-governmental organization (NGO) dedicated to improving the living standards of coastal communities and simultaneously placing a strong emphasis on the protection of marine ecosystems.
One of the key areas we work in is Artificial Reefs. This presentation captures our journey so far and our learnings. We hope you get as excited about marine conservation and artificial reefs as we are.
Please visit our website: https://kuddlelife.org
Our Instagram channel:
@kuddlelifefoundation
Our Linkedin Page:
https://www.linkedin.com/company/kuddlelifefoundation/
and write to us if you have any questions:
info@kuddlelife.org
Diabetes is a rapidly and serious health problem in Pakistan. This chronic condition is associated with serious long-term complications, including higher risk of heart disease and stroke. Aggressive treatment of hypertension and hyperlipideamia can result in a substantial reduction in cardiovascular events in patients with diabetes 1. Consequently pharmacist-led diabetes cardiovascular risk (DCVR) clinics have been established in both primary and secondary care sites in NHS Lothian during the past five years. An audit of the pharmaceutical care delivery at the clinics was conducted in order to evaluate practice and to standardize the pharmacists’ documentation of outcomes. Pharmaceutical care issues (PCI) and patient details were collected both prospectively and retrospectively from three DCVR clinics. The PCI`s were categorized according to a triangularised system consisting of multiple categories. These were ‘checks’, ‘changes’ (‘change in drug therapy process’ and ‘change in drug therapy’), ‘drug therapy problems’ and ‘quality assurance descriptors’ (‘timer perspective’ and ‘degree of change’). A verified medication assessment tool (MAT) for patients with chronic cardiovascular disease was applied to the patients from one of the clinics. The tool was used to quantify PCI`s and pharmacist actions that were centered on implementing or enforcing clinical guideline standards. A database was developed to be used as an assessment tool and to standardize the documentation of achievement of outcomes. Feedback on the audit of the pharmaceutical care delivery and the database was received from the DCVR clinic pharmacist at a focus group meeting.
"Understanding the Carbon Cycle: Processes, Human Impacts, and Strategies for...MMariSelvam4
The carbon cycle is a critical component of Earth's environmental system, governing the movement and transformation of carbon through various reservoirs, including the atmosphere, oceans, soil, and living organisms. This complex cycle involves several key processes such as photosynthesis, respiration, decomposition, and carbon sequestration, each contributing to the regulation of carbon levels on the planet.
Human activities, particularly fossil fuel combustion and deforestation, have significantly altered the natural carbon cycle, leading to increased atmospheric carbon dioxide concentrations and driving climate change. Understanding the intricacies of the carbon cycle is essential for assessing the impacts of these changes and developing effective mitigation strategies.
By studying the carbon cycle, scientists can identify carbon sources and sinks, measure carbon fluxes, and predict future trends. This knowledge is crucial for crafting policies aimed at reducing carbon emissions, enhancing carbon storage, and promoting sustainable practices. The carbon cycle's interplay with climate systems, ecosystems, and human activities underscores its importance in maintaining a stable and healthy planet.
In-depth exploration of the carbon cycle reveals the delicate balance required to sustain life and the urgent need to address anthropogenic influences. Through research, education, and policy, we can work towards restoring equilibrium in the carbon cycle and ensuring a sustainable future for generations to come.
Micro RNA genes and their likely influence in rice (Oryza sativa L.) dynamic ...Open Access Research Paper
Micro RNAs (miRNAs) are small non-coding RNAs molecules having approximately 18-25 nucleotides, they are present in both plants and animals genomes. MiRNAs have diverse spatial expression patterns and regulate various developmental metabolisms, stress responses and other physiological processes. The dynamic gene expression playing major roles in phenotypic differences in organisms are believed to be controlled by miRNAs. Mutations in regions of regulatory factors, such as miRNA genes or transcription factors (TF) necessitated by dynamic environmental factors or pathogen infections, have tremendous effects on structure and expression of genes. The resultant novel gene products presents potential explanations for constant evolving desirable traits that have long been bred using conventional means, biotechnology or genetic engineering. Rice grain quality, yield, disease tolerance, climate-resilience and palatability properties are not exceptional to miRN Asmutations effects. There are new insights courtesy of high-throughput sequencing and improved proteomic techniques that organisms’ complexity and adaptations are highly contributed by miRNAs containing regulatory networks. This article aims to expound on how rice miRNAs could be driving evolution of traits and highlight the latest miRNA research progress. Moreover, the review accentuates miRNAs grey areas to be addressed and gives recommendations for further studies.
Willie Nelson Net Worth: A Journey Through Music, Movies, and Business Venturesgreendigital
Willie Nelson is a name that resonates within the world of music and entertainment. Known for his unique voice, and masterful guitar skills. and an extraordinary career spanning several decades. Nelson has become a legend in the country music scene. But, his influence extends far beyond the realm of music. with ventures in acting, writing, activism, and business. This comprehensive article delves into Willie Nelson net worth. exploring the various facets of his career that have contributed to his large fortune.
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Introduction
Willie Nelson net worth is a testament to his enduring influence and success in many fields. Born on April 29, 1933, in Abbott, Texas. Nelson's journey from a humble beginning to becoming one of the most iconic figures in American music is nothing short of inspirational. His net worth, which estimated to be around $25 million as of 2024. reflects a career that is as diverse as it is prolific.
Early Life and Musical Beginnings
Humble Origins
Willie Hugh Nelson was born during the Great Depression. a time of significant economic hardship in the United States. Raised by his grandparents. Nelson found solace and inspiration in music from an early age. His grandmother taught him to play the guitar. setting the stage for what would become an illustrious career.
First Steps in Music
Nelson's initial foray into the music industry was fraught with challenges. He moved to Nashville, Tennessee, to pursue his dreams, but success did not come . Working as a songwriter, Nelson penned hits for other artists. which helped him gain a foothold in the competitive music scene. His songwriting skills contributed to his early earnings. laying the foundation for his net worth.
Rise to Stardom
Breakthrough Albums
The 1970s marked a turning point in Willie Nelson's career. His albums "Shotgun Willie" (1973), "Red Headed Stranger" (1975). and "Stardust" (1978) received critical acclaim and commercial success. These albums not only solidified his position in the country music genre. but also introduced his music to a broader audience. The success of these albums played a crucial role in boosting Willie Nelson net worth.
Iconic Songs
Willie Nelson net worth is also attributed to his extensive catalog of hit songs. Tracks like "Blue Eyes Crying in the Rain," "On the Road Again," and "Always on My Mind" have become timeless classics. These songs have not only earned Nelson large royalties but have also ensured his continued relevance in the music industry.
Acting and Film Career
Hollywood Ventures
In addition to his music career, Willie Nelson has also made a mark in Hollywood. His distinctive personality and on-screen presence have landed him roles in several films and television shows. Notable appearances include roles in "The Electric Horseman" (1979), "Honeysuckle Rose" (1980), and "Barbarosa" (1982). These acting gigs have added a significant amount to Willie Nelson net worth.
Television Appearances
Nelson's char
Natural farming @ Dr. Siddhartha S. Jena.pptxsidjena70
A brief about organic farming/ Natural farming/ Zero budget natural farming/ Subash Palekar Natural farming which keeps us and environment safe and healthy. Next gen Agricultural practices of chemical free farming.
Characterization and the Kinetics of drying at the drying oven and with micro...Open Access Research Paper
The objective of this work is to contribute to valorization de Nephelium lappaceum by the characterization of kinetics of drying of seeds of Nephelium lappaceum. The seeds were dehydrated until a constant mass respectively in a drying oven and a microwawe oven. The temperatures and the powers of drying are respectively: 50, 60 and 70°C and 140, 280 and 420 W. The results show that the curves of drying of seeds of Nephelium lappaceum do not present a phase of constant kinetics. The coefficients of diffusion vary between 2.09.10-8 to 2.98. 10-8m-2/s in the interval of 50°C at 70°C and between 4.83×10-07 at 9.04×10-07 m-8/s for the powers going of 140 W with 420 W the relation between Arrhenius and a value of energy of activation of 16.49 kJ. mol-1 expressed the effect of the temperature on effective diffusivity.
Characterization and the Kinetics of drying at the drying oven and with micro...
detect and identify common human bacterial pathogens in high purity water.
1. A rapid culture independent methodology to
quantitatively detect and identify common
human bacterial pathogens associated with
contaminated high purity water
BY: SAAD FAROOQI
ABDUR REHMAN
JUNAID GHANI
FAWAD KHAN
2. Background
• Water and High Purity Water (HPW) distribution systems can be
contaminated with human pathogenic microorganisms.
• This biocontamination may pose a risk to human health as HPW is
commonly used in the industrial, pharmaceutical and clinical sectors.
• Currently, routine microbiological testing of HPW is performed using
slow and labour intensive traditional microbiological based
techniques.
• There is a need to develop a rapid culture independent methodology
to quantitatively detect and identify biocontamination associated
with HPW.
3. methods
• Bacterial strains, microbial culture media and growth conditions
Stenotrophomonas species,
Burkholderia species,
Pseudomonas species
Serratia species were used in this study
• DNA isolation and quantification
Total genomic DNA was isolated from overnight broth culture using the Quick-gDNA MiniPrep kit.
DNA integrity was assessed on a 1% w/v agarose gel. Concentrations for all total genomic DNA used
in this study were determined using the Quant-iT™ dsDNA HS Assay Kit and the Qubit
fluorometer
• Real-time PCR
Multiplex real-time PCR reactions were subsequently performed using the LightCycler 480 probes
master kit
4. Results
• A novel internally controlled 5-plex real-time PCR Nucleic
Acid Diagnostics assay (NAD), was designed and optimised in
accordance with Minimum Information for Publication of
Quantitative Real-Time PCR Experiments guidelines, to
rapidly detect, identify and quantify the human pathogenic
bacteria Stenotrophomonas maltophilia, Burkholderia
species, Pseudomonas aeruginosa and Serratia marcescens
which are commonly associated with the biocontamination
of water and water distribution systems.
5. CONTD
• The presence of any DNA from pathogenic microorganisms in a water
sample indicates that there is biological contamination within the
system.
• Any contamination with pathogenic microorganisms within water
distribution systems even if present at low concentrations should be
considered a risk.
• This current study, has focused on the development of a rapid
methodology for the absolute quantification, detection and
identification of four pathogenic bacteria from an operating HPW
distribution system.
6. conclusion
• The novel culture independent methodology described in
this study allows for rapid (<5 hours), quantitative detection
and identification of these four human pathogens from
biocontaminated water and HPW distribution systems.
• Conventional methods takes 2 to 7 days
• We propose that the described NAD assay and associated
methodology could be applied to routine testing of water
and HPW distribution systems to assure microbiological
safety and high water quality standards.
7. conclusion
• The novel culture independent methodology described in
this study allows for rapid (<5 hours), quantitative detection
and identification of these four human pathogens from
biocontaminated water and HPW distribution systems.
• Conventional methods takes 2 to 7 days
• We propose that the described NAD assay and associated
methodology could be applied to routine testing of water
and HPW distribution systems to assure microbiological
safety and high water quality standards.