Analgesic activity of drug using eddy's hot plate method

Analgesic activity of drug using eddy's hot plate method

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  AIM: TO STUDY ANALGESICACTIVITY OF DRUG USING EDDY’S HOT PLATE ANALGESIOMETER IN MICE REFERENCE HANDBOOK OF EXPERIMENTAL PHARMACOLOGY BY S.K.KULKARNI,3rd EDITION, REPRINT 2007, VALLABH PRAKASHAN REQUIREMENTS INSTRUMENT- EDDY’S HOT PLATE DRUGS- MORPHINE SULPHATE APPARATUS- SYRINGE,BEAKER,ETC ROUTE-SUBCUTANEOUS
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  PRINCIPLE In this methodheat is used as a source of pain. Animals are individually placed on a hot plate maintained at constant temperature(55ºc) and the reaction of animal such as hind paw licking or jump response is taken as the end point. Analgesic increases the reaction time This method was described by Eddy and Leimbach in 1953
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  PROCEDURE  Weigh andnumber the mice  Take the basal reaction time by observing hind paw licking or jump response(whichever appeals first) in animals when placed on the hot plate maintained at constant temperature(55ºc). Normally animals show such response in 6-8sec. A cut off period of 15sec is observed to avoid damage to paws.  Inject morphine to animals and note the reaction time of animals on the hot plate at 15,30, 60 and 120 min after the drug administration. As the reaction time increases with morphine, 15sec is taken as maximum analgesia and the animals are removed from the hot plate  Calculate % increase in reaction time(as index of analgesia) at each time innterval.
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  OBSERVATION BASAL REACTION (PAWLINK) TIME(SEC) JUMP AFTER DRUG PAWLINK AFTER DRUG JUMP
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  RESULT The analgesic effectof morphine on mice was studied by using hot plate method