here i am sharing my ppt presentation on DSC .

1. Differential scanning cALORImetry
Presented by
SUMAN DHAURIA
M PHARM(PHARMACEUTICS) , 1st SEMESTER
ADAMAS UNIVERSITY
Under the guidance of
DR. ASIM HALDER
DR JHANSI LAKSHMI PARIMI
ASSISTANT PROFESSOR , ADAMAS UNIVERSITY

2.  Introduction
 History of DSC
 Principle of DSC
 Typical DSC Curve
 Types of DSC
 Instrumentation
 Application of DSC

3. DIFFERNTIAL SCANNING CALORIMETRY:-
. A thermoanalytical technique in which, the difference in the amount of heat
required to increase the temperature of a sample and reference is measured as a
function of temperature.
. Both the sample and reference are maintained at nearly the same temperature
throughout the experiment.
. Powerful experimental technique used for determining thermodynamic of solid
and liquid phase transitions that produce or absorb heat.

4. The technique was developed by E . S . Watson and M. J .O’Neill in 1962 and introduced commercially at the
1963 Pittsburgh conference on analytical chemistry and applied spectroscopy.

5. TYPICAL DSC CURVE
TYPICAL DSC CURVE

6. M
 The basic principle of this techniques is that ,when the sample undergoes a physical transformation
such as; phase transitions more and less heat will need to flow to it than the reference to maintain
both at the same temperature.
 Whether less or more heat must flow to the sample depends on whether the process is exothermic
and endothermic .
 For example , as a solid sample melts to a liquid ,it will require more heat flowing to the sample to
increase its temperature at the same rate as the reference .
 DSC has become the most widely used thermal analysis technique ,in which the sample and
reference material are subjected to a precisely programmed temperature change.

7. TYPES OF DSC
There are two different types of DSC
1. Heat - flux DSC- For this kind of experiment , a sample and reference crucible are placed on a sample holder
with integrated temperature sensors for measurement of the crucibles .
2. Power differential DSC- For this kind of experiment ,the sample and reference crucible are placed in thermally
insulated furnaces and no next to each other in the same furnace like heat flux DSC experiment .

8. INSTRUMENTATION
DSC apparatus consist of :
1. Furnace 4. Gas control device
2. Signal Amplifier 5. Differential detector
3. Temperature controller 6. Recorder

10. APPLICATIONS OF DSC
1. Protein stability and folding .
2. Liquid biopharmaceutical Formulations .
3. process Development .
4. Protein Engineering .
5. Antibody Domain Studies .
6. characterization of membranes ,liquid, nucleic acids and micellar systems .
7. Measurement of ultra-light molecular interactions.
8. Rank order Binding.
9. DSC in pharmaceutical industry
Detection of polymorphism.
Detection of isomerism.
purity determination of sample directly
Finger printing of wax
Lipid and protein determination.