Different method of sterilization
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This document discusses different methods of sterilization, including physical and chemical methods. Physical sterilization methods include dry heat (such as sunlight, hot air ovens), moist heat (such as autoclaving, pasteurization), radiation (such as UV, gamma rays), and filtration. Chemical sterilization methods involve the use of gaseous agents like ethylene oxide or disinfectants like phenol and alcohol. Specific sterilization techniques are described, along with their advantages and limitations. The document provides an overview of common sterilization processes.
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Dr. Ibrahim presented on various sterilization methods including physical agents like heat, radiation, and filtration as well as chemical agents like alcohols, aldehydes, and gases. The most common sterilization methods used in laboratories are moist heat using an autoclave at 121°C for 15 minutes or 134°C for 3 minutes, dry heat using a hot air oven at 160-180°C, and chemical methods using ethylene oxide or beta propiolactone gases. Proper testing of sterilization efficacy includes using chemical, mechanical, and biological indicators to confirm sterilizing conditions have been met.
4. sterilisation
The document discusses various methods of sterilization and disinfection. It defines sterilization as a process that eliminates all microorganisms, including bacterial spores, while disinfection only reduces microorganisms to safe levels. Several physical methods are described including heat sterilization using hot air ovens, autoclaving with moist heat, and radiation. Chemical methods involving the use of alcohols, aldehydes, phenols, halogens, metallic salts, dyes and surface active agents are also outlined. The mechanisms of action and appropriate uses of different sterilization and disinfection techniques are provided in detail over the course of the document.
Sterlization chapter
This chapter contains sterilization techniques : physical - heat , filtration and radiations and chemical methods of sterilization in simple concise way so that mode of action is understood .
sterilisation-.pptx
Dr. Ibrahim presented on various sterilization methods including physical agents like heat, radiation, and filtration as well as chemical agents like alcohols, aldehydes, and gases. The most common sterilization methods used in laboratories are moist heat using an autoclave at 121°C for 15 minutes or 134°C for 3 minutes, dry heat using a hot air oven at 160-180°C, and chemical methods using ethylene oxide or beta propiolactone gases. Proper testing of sterilization efficacy includes using chemical, mechanical, and biological indicators to confirm sterilizing conditions have been met.
STERILIZATION IN PROSTHODONTICS
Sterilization: It is defined as the process by which an article, surface or medium is freed of all living microorganisms either in the vegetative or spore state.
Disinfection: The destruction or removal of all pathogenic organisms, or organisms capable of giving rise to infection.
Antisepsis: The prevention of infection , usually by inhibiting the growth of bacteria in wounds or tissues.
Sterilization.pptx
This document discusses sterilization methods. It begins by outlining the unit outcomes which are to understand different sterilization methods, equipment used for large scale sterilization, and techniques for evaluating sterilization efficiency. It then covers various sterilization concepts and classifications of physical and chemical sterilization methods. Specific physical methods discussed in detail include dry heat sterilization using hot air ovens, and moist heat sterilization using autoclaves. Radiation sterilization using UV light is also summarized. The document provides information on sterilization principles, advantages, disadvantages, and applications of different methods.
sterilization.pdf
The document discusses various methods of sterilization and disinfection. It defines sterilization as a process that kills all microorganisms including bacterial spores, while disinfection eliminates most pathogens but not spores. Physical sterilization methods discussed include heat, radiation and filtration. Heat sterilization can be achieved through moist heat methods like autoclaving or dry heat methods like hot air ovens. Radiation sterilization uses ionizing or non-ionizing rays. The document also discusses various chemical disinfectants and differences between related processes like sanitization, asepsis and antisepsis.
sterilization.pdf
The document discusses various methods of sterilization and disinfection. It defines sterilization as a process that kills all microorganisms including bacterial spores, while disinfection eliminates most pathogens but not spores. Physical sterilization methods discussed include heat, radiation and filtration. Heat sterilization can be achieved through moist heat methods like autoclaving or dry heat methods like hot air ovens. Radiation sterilization uses ionizing or non-ionizing rays. The document also discusses various chemical disinfectants and differences between related processes like sanitization, asepsis and antisepsis.
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principles of sterilization, concepts, various types of sterilization methods
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Different method of sterilization
- 1. Different Method of Sterilization Prepared By Abhijeet B. Pharm{2019-2023}
- 2. CONTENTS Introduction Methods of Sterilization
- 3. INTRODUCTION Sterilization is a broad term that refers to any process that removes or kills all forms of microorganisms. This include bacteria, fungi, viruses and protozoans.
- 4. Method of Sterilization Various Methods of Sterilization are as following: 1. Physical Method a. Dry heat sterilization b. Moist heat sterilization c. Sterilization by Radiation d. Filtration 2. Chemical Method a. Gaseous Method b. by using disinfectants
- 5. PHYSICAL METHODS: A. Dry heat sterilization Heat is the most reliable and rapid method of sterilization. Dry heat kills microorganism by causing protein denaturation and destructive oxidation of essential cell.
- 6. Various methods of sterilization by dry heat include: 1. Sunlight: Direct sunlight has sterilizing effect due to presence of UV rays and heating effect of infra red rays. 2. Red Heat: Inoculating wires, loop, spatulas and forceps are sterilized by holding them on a flame of Bunsen burner. 3. Flaming: Glass slides, cover slip, needles are sterilized by Passing them over the flame of Bunsen burner without allowing it to become red hot. 4. Incineration: Efficient method for destroying infective material like pathological material or contaminated cloth by reducing them to ashes by burning.
- 7. Contd. Dry heat 5. Hot air oven: It is widely used method of sterilization by dry heat. It is electrically heated and thermostatically controlled by exposing material at 160c for 1 hour.
- 8. Moist Heat Sterilization B. Moist heat sterilization Killing of microorganism with hot water or steam. Divided into three forms in terms of temperature: 1. Temperature below 100c: Pasteurization: It is a process in which milk is heated and cooled immediately It is also used for ice cream, yogurt and fruit juices. Two methods are generally used:
- 9. Contd. Moist Heat Sterilization a. Holder method: 62.8C for 30 minute. b. Flash method: 72C for 20 seconds
- 10. Contd. Moist Heat Sterilization 2. Temperature at 100C : Tyndallization: Steam at 100C for 20 minutes on three successive days. Used for egg, serum and sugar containing media. Boiling: Boiling for 10-30 minutes kills mostly the vegetative form of bacteria. 3. Temperature above 100C: Autoclave: It is a modified pressure cooker in which sterilization is achieved by saturated steam under high pressure at 121C for 15 minutes.
- 11. Sterilization by Radiation C. Sterilization by Radiation Two types of radiation: Ionizing and Non-ionizing radiation. 1. Non-ionising radiation(Hot sterilization) Infrared- Used for rapid mass sterilization of pre-packed items such as syringe. UV- Used for disinfecting enclosed area such as hospitals and labs.
- 12. Contd. Sterilization by Radiation 2. Ionising sterilization(Cold sterilization) Done by using Gamma rays and X rays. Used for sterilising plastics, syringes, swabs, fabric and metal foils.
- 13. Filtration Method D. Filtration This method is used for the sterilization of liquid substance or fluid such as sera and solutions of heat labile substance. Types of filter: 1. Candle filter 2. Asbestos filter 3. Sintered glass filter 4. Membrane filter
- 14. Contd. Filtration Method 1. Candle filter It is widely used for the purification of water for industrial and drinking purposes. 2. Asbestos filter It is disposable and single disc made up of asbestos(magnesium trisilicate) Pore size of filter ranges from 0.01 to 5micron.
- 15. Contd. Filtration Method 3. Sintered glass filter It has low absorptive properties They are brittle and expensive . 4. Membrane filter Made up of cellulose esters and other polymers, Usually used for the purification of water and sterile testing for parenteral use.
- 16. CHEMICAL METHODS: 1. Gaseous Method The most common gas used in gaseous sterilization is ethylene oxide. It is a colourless liquid with BP 10.8. Ethylene oxide is widely used gaseous sterilizing agent in medical science. ADVANTAGES- It is powerful sterilizing agent for surgical instruments. DISADVANTAGES- Low sterilization process.
- 17. 2. By using disinfectants Phenol, alcohol, dyes, aldehydes, etc. can be used as a disinfectant or antimicrobial agent.