It comprises on mating designs used in plant breeding programs. 6 basic mating designs are briefly explained in it with their requirements as well limiting factors...
GPB 311: Maize- Centre of origin, distribution of species, wild relatives and major breeding objectives and procedures for development of varieties and hybrids for improvement yield, adoptability, stability, biotic and abiotic stress tolerance and quality of Maize
It comprises on mating designs used in plant breeding programs. 6 basic mating designs are briefly explained in it with their requirements as well limiting factors...
GPB 311: Maize- Centre of origin, distribution of species, wild relatives and major breeding objectives and procedures for development of varieties and hybrids for improvement yield, adoptability, stability, biotic and abiotic stress tolerance and quality of Maize
Hybridization between individuals from different species belonging to the same genus or two different genera, is termed as distant hybridization or wide hybridization, and such crosses are known as distant crosses or wide crosses.
Stability analysis and G*E interactions in plantsRachana Bagudam
Gene–environment interaction is when two different genotypes respond to environmental variation in different ways. Stability refers to the performance with respective to environmental factors overtime within given location. Selection for stability is not possible until a biometrical model with suitable parameters is available to provide criteria necessary to rank varieties / breeds for stability. Different models of stability are discussed.
Hybridization between individuals from different species belonging to the same genus or two different genera, is termed as distant hybridization or wide hybridization, and such crosses are known as distant crosses or wide crosses.
Stability analysis and G*E interactions in plantsRachana Bagudam
Gene–environment interaction is when two different genotypes respond to environmental variation in different ways. Stability refers to the performance with respective to environmental factors overtime within given location. Selection for stability is not possible until a biometrical model with suitable parameters is available to provide criteria necessary to rank varieties / breeds for stability. Different models of stability are discussed.
The mating or crossing of two plants or lines of dissimilar genotype is known as hybridization. The chief objective of hybridization is to create genetic variation, when two genotypically different plants are brought together in F1. Here, we are going to discuss about different strategies and tools used for hybridization.
Heterosis breeding
Heterosis or hybrid vigour or outbreeding enhancement
Types of heterosis
Genetic basis of heterosis
HYBRIDS
Development of inbreds
Combining ability
Types of hybrids
Single cross hybrid
Double cross hybrid
Triple cross hybrid
Top cross hybrid
This ppt illustrate about various breeding method used in cross pollinated crops. best breeding methods available for cross pollinated crops. basic introduction of cross pollination mechanism.
STUDY OF MORPHOLOGICAL AND YIELD ATRIBUTING CHARACTERS IN INDIGENOUS RICE (OR...Vipin Pandey
The present study was carried out to study ninety four rice accessions, along with checks, on the basis of sixteen
qualitative and twenty quantitative characters. Analysis of variance for quantitative characters showed differences for
different characters. High coefficient of variation in the entire genotypes was observed for grain yield per plant (27.4 %),
number of effective tillers per plant (22.37 %), test weight (21.14 %) and kernel length breadth ratio (20.59 %).
Correlation analysis revealed positive and highly significant correlation of total number of filled grains per panicle, total
number of grains per panicle, plant height and number of effective tiller per plant; harvest index, test weight, flag leaf
length and days to maturity had positive highly significant correlation with grain yield per plant. Principal Component
Analysis revealed, out of 20, only seven principal components (PCs) exhibited more than 1.00 eigen value, and showed
about 77.42 % variability among the traits studied. So, these 7 PCs were given due importance for further explanation.
Component matrix revealed that the PC1 was mostly related to quality characters while PC2, PC3, PC4, PC5, PC6 and
PC7 mostly associated with yield related traits. Cluster analysis performed by UPGMA method using Euclidean distance
as dissimilarity measure divided the 97 genotypes of rice into ten clusters. The cluster III constituted of 48 genotypes,
forming the largest cluster followed by cluster VI (22 genotypes), cluster V (10 genotypes), cluster II (5 genotypes) and
cluster VIII (4 genotypes), cluster I, IV and VII (two genotypes each), cluster IX and X had (only one genotypes each).
Quality analysis performed for 97 rice genotypes revealed wide range of genetic variability for most of the quality traits.
Inter specific hybridization to introduce useful genetic variability for pig...Vipin Pandey
Pulses occupy an important place in Indian agriculture. Within this protein-rich group of crops, red gram or pigeonpea occupies an important place among rainfed resource poor farmers because it provides quality food, fuel wood, broom and fodder.
Hybrids are plants that result from controlled cross-breeding of two different but specific varieties or breeding lines of the same species of plant. Wild species are important sources of resistance to biotic and abiotic stresses as they have evolved to survive droughts, floods, extremes of temperature (heat/ cold) and have the capability to with stand damage by insect pests and diseases. Ten alleles reported unique to inter-specific derivatives of Cajanus cajan × C. scarabaeoides. The presence of alleles unique to specific population or group indicates an inimitable genetic variability at certain loci. This information is valuable to categorise interspecific hybrids with exclusive genetic variability, whose selection can increase the allele richness of breeding population (Saxena, 2015).
High levels of resistance is available in wild Cajanus species, these are not being utilised adequately in pigeonpea breeding programs. The major limitation is due to the linkage drag and different incompatibility barriers between cultivated and wild species. Under such situations, pre-breeding provides a unique opportunity to expand primary gene pool by exploiting genetic variability present in wild species and cultivated germplasm and will ensure continuous supply of new and useful genetic variability into the breeding pipelines to develop new cultivars having high levels of resistance and broad genetic base (Sharma et al., 2013). The major limitation in successfully using Cajanus platycarpus for the improvement of cultivated pigeonpeais embryo abortion in the BC1 generation from the cross C. Platycarpus × C. cajan. This Cajanus platycarpus, although placed in the tertiary gene pool of pigeonpea, is now amenable to gene transfer with the development of suitable embryo rescue techniques (Mallikarjuna et al., 2011).
TOPIC:TRANSGENIC CROPS AND THEIR IMPLICATION IN ENVIRONMENT AND FOOD SAFETYVipin Pandey
Transgenes means genetically modified genesThe term transgenic was first used by Gordon and Ruddle in 1981.
Transgenic crops are plants that have been genetically engineered, a breeding approach that uses recombinant DNA techniques to create plants with new characteristics. They are identified as a class of genetically modified organism (GMO)
Problems and prospects of hybrid pigeonpea in india Vipin Pandey
Pulses occupy an important place in Indian agriculture. Within this protein-rich group of crops, red gram or pigeonpea occupies an important place among rainfed resource poor farmers because it provides quality food, fuel wood and fodder.
Pigeonpea breeding started at 1933, first time studied morphological and agronomic traits of 86 elite indigenous pigeonpea germplasm accessions and they find some of the accessions were having high level of resistance to wilt (Shaw et al., 1933).
Hybrids are plants that result from controlled cross-breeding of two different but specific varieties or breeding lines of the same species of plant. Male pollen is transferred to the female pistil to achieve pollination, thus forming a seed. The result is what is called an F1 hybrid. Male sterility is refers to a condition in which pollen is either absent or non-functional in flowering plants. Hybrid seed production of pigeonpea are using
Genetic Male Sterility and first hybrid variety of pigeonpea are released but some problems related to Genetic Male Sterility based hybrid seed production are low amount of hybrid seed production (50%), roughing of fertile counterpart of female (cost increasing), lack of necked eye marker for male sterility (linked marker), difficult to maintain genetic purity (Saxena, K. B., 2015).
The per capita availability of protein in the country is already one third of its requirement and cultivated area are also decreased it is important to enhance its productivity in nearly future. So future prospects of hybrid pigeonpea are, we can make more stable hybrid, we can use wild relatives for stress tolerance breeding (Choudhary et al., 2011), utilize the genomic resources and breeding for special traits. Pigeonpea has a genome size 833Mb and is the first non-industrial food legume crop for which draft genome sequence has been developed (Varshney et al., 2012).
Molecular genetics: it deals with the structure, composition, function and replication of chromosomes and genes, representing genetics material like DNA and RNA.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
1. Development of Hybrid
Varieties in Cross
Pollinated Crops
Speaker: Vipin Kumar Pandey (Ph.D. Scholar)
CLASS PRESENTATION (GP-603)
Presentation Date: 12-1-2018
2. INTRODUCTION
• The two most important features of cross pollinated species are:-
Inbreeding depression and
Heterosis
• In population improvement scheme for cross pollinated species are:
Keeping low level of inbreeding depression
High Heterosis
• In the hybrid varieties utilize full Heterosis while synthetic varieties
utilize a part of Heterosis.
Development of hybrid varieties differs from species to species. The
production of hybrid varieties in maize consists of three main steps.
I) Development of inbreds.
II) Selection of productive inbreds and
III) Production of hybrid seed.
3. I) Development of Inbreds:
It is an important step in the production of hybrids. There are two methods of
developing inbred lines
By selfing of heterozygous population and
By doubling of haploids.
In 1909 Shull suggested that inbreds should be developed from
open pollinated varieties by continued selfing.
Difficulties of shulls schem:-
Unavailability of outstanding inbrids.
Hybrid seed produced per acre was low.
Pollen production was low.
Poorly hybrid seed developed.
Double cross scheme proposed by Jones in 1918.
4. Various population viz. open pollinated varieties, synthetic
varieties or any other heterozygous population can be used
for selfing.
Progeny of selected plants are grown separately from the
selfed seed in the next season.
Again selection is made for the superior progeny and selfed.
This processes is continue to get superior homozygous
inbred.
Developing inbred lines by selfing of heterozygous
population
5.
6.
7.
8. Evaluation and Selection of Inbreds Lines:
The inbred is evaluated from its performance in hybrid combination with other
inbreds.
They are evaluated on the basis of their general combining ability and specific
combing ability.
a) Top Cross Method:
Davis suggested this method in 1927. It refers to a cross between an inbred line and an
open pollinated variety. Single inbred lines say 100 are crossed to a common tester (
Open pollinated variety ) to produce 100 single crosses. The yield performance of these
crosses is evaluated in replicated trails on multi locations. The line, which produces high
yielding single cross with tester, are selected. Inbred line which give high yield in top
crosses generally produce high yielding single crosses.
b) Single Cross Method:
This method is used to measure the specific combining ability ( SCA) of those inbreds,
which are selected on the basis of top cross performance. The selected lines are crossed
in all possible combination. These single crosses are evaluated in replicated trials over
several locations for yield performance. The best performing single crosses are identified
for release as a variety. This method can evaluate only limited number of inbreds at a
time.
9. Time of Testing:
The testing of inbred for general combining ability should be started from 3 rd, 4 th, and
5 th generation of selfing. This will help in retaining of inbreds with good combining
ability and elimination of lines with poor combining ability.
10. III) Production of Hybrid Seed:
After identification of superior line of the hybrid seed is produced. There are three types
of Intervarietal hybrid.
a) Single cross hybrid,
b) b) Three way cross hybrid and
c) c) Double cross hybrid.
In case of single and three way cross hybrids, the rows of female
and male parents are planted in 2:1 ratio. The tassels of female
parent are removed and natural cross pollination is allowed.
In case of three way cross hybrid, the single cross hybrid is used as
female parent and inbred lines as male parent.
While in case of double cross hybrid , the rows of female and male
parent are planted in 3:1 or 4:1 ratio.
The seed production is carried out in isolation to prevent crossing
with other compatible genotypes and maintain the high genetic
purity.
11. References
• Allard, R. W. 1960. Principles of plant breeding. John Wiley and Sons, inc, new
York.
• Comstock, R. E., Robinson, H. F. and Harvey P. H. 1949. A breeding procedure
designed to make maximum use of both general and specific combining ability.
Agron. J. 41: 360-367.
• Singh, B.D. 2012. Plant breeding principles and methods, Kalyani
Publishers, New Delhi-110002.
• www.agriinfo.in/default.aspx?page=topic&superid=3&topicid=1773