coombs test, introduction with principle and whole laboratory procedure, also u will read about ,how to perform direct and indirect coombs test? and how to report them?
ABO blood group system was decover by Karal landsteine
which contain A, B, and o antigen on the surface of BC, WBC,s platatelet and other body tissue cells except brain cell, and anti A, antiB and Anti Ab natural occuring antibodies in plasma of B,A, and O blood group individual respectively
ABO blood group system was decover by Karal landsteine
which contain A, B, and o antigen on the surface of BC, WBC,s platatelet and other body tissue cells except brain cell, and anti A, antiB and Anti Ab natural occuring antibodies in plasma of B,A, and O blood group individual respectively
Rh typing and its technique , BLOOD TYPING , Rhesus (Rh) typing , procedures of rh typing, process of Rh typing, Test limitations, Sources of Error in Rh Antigen Typing, False positive reactions' reason, False negative reactions' reasons
Direct
Passive
Reverse Passive
Agglutination Inhibition
Coagglutination
Agglutination tests can be done :
On slides
In tubes
In microtritation plates
-Difference between precipitation and agglutination reaction.
Rh typing and its technique , BLOOD TYPING , Rhesus (Rh) typing , procedures of rh typing, process of Rh typing, Test limitations, Sources of Error in Rh Antigen Typing, False positive reactions' reason, False negative reactions' reasons
Direct
Passive
Reverse Passive
Agglutination Inhibition
Coagglutination
Agglutination tests can be done :
On slides
In tubes
In microtritation plates
-Difference between precipitation and agglutination reaction.
In this ppt, discussing about pathogenesis of Corynebacterium diphtheriae and Mycobacterium tuberculosis and also included other types of lower respiratory tract infections causing microorganisms.
This ppt included all important points about food poisoning, these short and simple notes will provide you better knowledge about different types of fungal and chemical intoxications.
In this slide i have discussed all the conventional tools to diagnose parasitic infections and also included all the new diagnostic advances have came to deep tech world.
Clinical case presentation- Rh incompatibility.pptxArpitaChandra12
This is a clinical case with Rh incompatibility. A 10 days baby diagnosed with Rh incompatibility and also having Bacteremia and Klebsiella pneumoniae is causing nosocomial infection in NICU
In this PPT I have explained processing of sputum sample step by step, first I have discussed about different methods of sputum sample collection and then also discussed about different criteria of sputum sample rejection after gram stain .
in this PPT we will learn better about six different criteria of sputum sample rejection . In case of tuberculosis what is the criteria of sputum sample collection and processing also have been covered in this PPT.
I hope it will be helpful for you
thank you so much
Clinical case presentation, Proteus mirabilis.pptxArpitaChandra12
There is a clinical case presentation which have been reported in our Microbiology lab. A patient having UTI and the causative agent is Proteus mirabilis.
This PPT is sharing with you how to process urine sample in laboratories. This PPT also included right way to make proper diagnosis. It will be very useful for laboratory technicians to understand that what is correct way to process the urine sample.
Step by steps processing also accepting criteria of Kass technique to report the pathogenic organism in urine sample.
Facts about MYCOBACTERIUM TUBERCULOSIS-- BLOG ArpitaChandra12
A content writing Blog is covering topic Mycobacterium tuberculosis, it includes general features, pathogenesis, lab diagnosis and treatment of tuberculosis. You will also aware about the facts about Mycobacterium tuberculosis.
A original article presentation in journal club.
It gives you better idea how to present a research article.
A cross sectional study was conducted to compare two different methods first is rapid card test and other is real time pcr for the diagnosis of corona virus disease.
This is a research article presentation. I have prepared an original article for power point presentation, it will be helpful for you all to know how to present an original article on journal club.
Before doing research in any field it is very important to know the way of writing a research article . We should know which different points to remember at the time of research paper presentation .I have included all the headings which fulfill all the demands of a better way to present a research article on journal club.
Above ppt includes different types of disinfectants used in microbiology ,classification of disinfectants, and also it includes some important techniques like Plasma sterilization ,ETO sterilization and bleaching of water.
Basavarajeeyam is an important text for ayurvedic physician belonging to andhra pradehs. It is a popular compendium in various parts of our country as well as in andhra pradesh. The content of the text was presented in sanskrit and telugu language (Bilingual). One of the most famous book in ayurvedic pharmaceutics and therapeutics. This book contains 25 chapters called as prakaranas. Many rasaoushadis were explained, pioneer of dhatu druti, nadi pareeksha, mutra pareeksha etc. Belongs to the period of 15-16 century. New diseases like upadamsha, phiranga rogas are explained.
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
2 Case Reports of Gastric Ultrasound
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
Knee anatomy and clinical tests 2024.pdfvimalpl1234
This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
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2. An immunological test
To detect the presence of antibodies against the red blood
cells.
To diagnose Rh incompatibility by detecting Rh antibody from
mothers and baby serum.
3. When Rh negative Mother delivers a Rh positive
baby (Rh Ag +ve) , during birth, some Rh +ve RBCs
may pass from fetus to the maternal circulation and
may induce Rh Ab formation in mother
In future these Rh antibodies effect Rh positive
pregnancies.(cross the placenta)
Causes hemolytic diseases
Erythroblastosis Featalis
4. Antiglobulin - Anti IgG
Made by immunized rabbits with human gamma
globulin
Bind to the Fc portion of Rh antibody bound on
RBCs, resulting in visible agglutination.
5. Direct Coombs test- it detects Rh
antibodies bound to RBCs in vivo present
in baby’s serum by directly adding
commbs reagent.
Indirect Coombs test- It detects free Rh
antibodies present in maternal serum after
the first delivery of Rh positive baby.
8. Prepare normal saline-cell suspension (5%)
Take a test tube
950 µL Normal saline + 50µL sample
Mix it
Take another tube- Add 1-2 drop of suspension + Normal saline
Centrifuged it for 1min at 1000rpm
Discard supernatant
wash x3 (to remove unbound globulins)
9. A test tube- Add 2-3 drops of washed RBCs
+
Anti-Human sera (Coomb’s reagent)
Incubate it 30 min
after incubation
Observe for Agglutination
Macroscopically and Microscopically
10. Take one drop of RBCs on a glass slide
place a coverslip and observe clumping
of RBCs under microscope
Clumping of RBCs –
Incompatible(coomb’s test positive)
Scattered RBCs seen(Compatible)
- Negative
12. The gel column acts as a filter that traps agglutinated RBCs (if there
is Ag- Ab complex)
but they pass through the gel column during the centrifugation
based on size (If there is no Ag- Ab complex)
Procedure
Add Patients washed RBCs cell suspension (50 µL) into card
then centrifuged the card
incubate it for 30 mint on water bath
Observe the results
13. RBCs settle down on bottom of the well- Compatible
RBCs on the surface of the gel
- Incompatible
MF = Mixed field agglutination
14.
15. + - T
Requirements-
Normal saline
Patients serum (mother)
Centrifuge
Pipette
5 Test tubes
O+ pooled cells (Rh +ve)
Anti- D Reagent (Anti D IgG)
16. Steps
Preparation of O+ Pooled cells- by adding (three blood samples
of o+ ve blood group)
make cell suspension of O+ve blood
1. Take 2-3 drops of RBCs + 2/3 Normal saline
2. x3 wash RBCs, centrifuge(1000rpm, 2min)
discard supernatant
3. A tube- Add 950 µL NS + 50 µL Washed cells
(5% Cell suspension)
17. 50 µL from Cell suspension add on all three tubes
+ + +
1 drop Anti D 50 µL NS Patient 50 µL serum
Incubate it 1 hr at 37C then wash x3 with NS
Add Anti- Sera on Tube(T)
Incubate 5 mint
Observe Macro / Microscopically
+ - T