Studies on the Crude Extract of Moringa Oleifera Leaf for Preliminary Identif...iosrjce
IOSR Journal of Applied Chemistry (IOSR-JAC) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of applied chemistry and its applications. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Chemical Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Moringa is a plantfood of high nutritional value, ecologically and economically beneficial and readily available in the countries hardest hit by the food crisis. http://miracletrees.org/ http://moringatrees.org/
The bioactive phytochemicals in Gouania longipetala was determined using GCMS analysis. The
extract was prepared using Soxhlet`s extraction method and concentrated at 35oC in hot air oven. GCMS
analyzes phytochemicals in plant by demonstrating the structures of the chemical compounds in it. The gas
chromatogram showed the presence of eight phytochemicals. The molecular mass of the phytochemicals were
established based on the molecular ion in the mass spectra. Identification of the phytochemicals was based on
comparison with National Institute of Standards and Technology (NIST) database. The identified
phytochemicals with their peak area percentages are 11,14-octadecadienoic acid (1.72%), Hexadecanoic acid
also known as Palmitic acid (19.86%), 9,11-octadecadienoic acid (1.33%), 9,12,15-Octadecatrien-1-ol (2.92%),
9-Octadecenoic acid (56.40%), Ethyl palmitate (9.42%), 17-carboxyheptadec-9-en-1-ylium (1.70%) and
Glutaric acid, isobutyl 2-nitrophenyl ester (6.65%). These identified compounds exhibited the following
bioactivities; inhibition of uric acid, urine acidifiers, amino acid decarboxylase activity, arachidonic acid
inhibitor, oligosaccharide provider, decrease endothelial leukocyte and platelet adhesion . Gouania longipetala
therefore contain active phytochemicals that may be beneficial in pharmacognosy. We recommend further work
to be done on its isolation and synthesis.
Studies on the Crude Extract of Moringa Oleifera Leaf for Preliminary Identif...iosrjce
IOSR Journal of Applied Chemistry (IOSR-JAC) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of applied chemistry and its applications. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Chemical Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Moringa is a plantfood of high nutritional value, ecologically and economically beneficial and readily available in the countries hardest hit by the food crisis. http://miracletrees.org/ http://moringatrees.org/
The bioactive phytochemicals in Gouania longipetala was determined using GCMS analysis. The
extract was prepared using Soxhlet`s extraction method and concentrated at 35oC in hot air oven. GCMS
analyzes phytochemicals in plant by demonstrating the structures of the chemical compounds in it. The gas
chromatogram showed the presence of eight phytochemicals. The molecular mass of the phytochemicals were
established based on the molecular ion in the mass spectra. Identification of the phytochemicals was based on
comparison with National Institute of Standards and Technology (NIST) database. The identified
phytochemicals with their peak area percentages are 11,14-octadecadienoic acid (1.72%), Hexadecanoic acid
also known as Palmitic acid (19.86%), 9,11-octadecadienoic acid (1.33%), 9,12,15-Octadecatrien-1-ol (2.92%),
9-Octadecenoic acid (56.40%), Ethyl palmitate (9.42%), 17-carboxyheptadec-9-en-1-ylium (1.70%) and
Glutaric acid, isobutyl 2-nitrophenyl ester (6.65%). These identified compounds exhibited the following
bioactivities; inhibition of uric acid, urine acidifiers, amino acid decarboxylase activity, arachidonic acid
inhibitor, oligosaccharide provider, decrease endothelial leukocyte and platelet adhesion . Gouania longipetala
therefore contain active phytochemicals that may be beneficial in pharmacognosy. We recommend further work
to be done on its isolation and synthesis.
Cumulative effect of modified atmospheric packaging on the textural and chemi...SukhveerSingh31
Fruits and vegetables have been consumed by humans since ancient times. Scientific
investigations have proved that an increased consumption of fruits and vegetables is known to
reduce instances of cancer and cardiovascular mortality (Bhardwaj et al., 2014)
This is a study undertaken on nutrient compositions of and processed products development from Son Tra (Docynia indica. The in-depth study of some bioactive substances of son tra fruit and its processed product development was undertaken as part of understanding existing market value chains, and identifying the necessary interventions to improve the performance of son tra.
Characterization of the Volatile Components and Antimicrobial Properties of t...Premier Publishers
The characterization of the ethanol leaf extract of Uvaria chamae was carried out with the aim of identifying and determining the chemical compounds present in the extract. Initial phytochemical results showed the presence of flavonoids, saponins, tannins. Phenols, steroids and alkaloids. Interpreted spectrum obtained from the GC-MS revealed twelve absorption peaks. Peak 1 was identified as Benzene carboxylic acid with molecular weight of 122g and molecular formula C7H6O2. Similarly, peaks 2-12 were identified as, hexadecanoic acid methyl ester, hexadecanoic acid, 11-octadecenoic acid methyl ester, phytol, 6-octadecanoic acid, octadecanoic acid, tetradecanamide. hexadecanoic acid-2,3-dihydroxpropyl ester, 9-octadecenamide, 9,12-octadecadienoyl chloride and 13-octadecanal with corresponding molecular formulas of C17H34O2, C16H36O2, C19H36O2, C20H40O, C18H34O2, C18H36O2, C14H29NO, C19H38O4, C18H35NO, C18H31ClO and C18H34O respectively. The extract inhibited the growth of some selected human pathogen; Pseudomonas aureginosa by 6mm with minimum inhibitory concentration (MIC) of 50mg/cm3. Similar results were also obtained for Candida albicans 8mm with MIC 50mg/cm3 and Trichophyton spp 4mm with MIC OF 100mg/cm3
Seaweed (Hypnea musciformis) Versus Freshwater Weed (Ipomoea aquatica): A Com...SaadAldin2
An undergraduate Thesis work accomplished in the dept. of Food Engineering and Tea Technology. This is the Defense power-point slide of the main Paper.
Analysis of phytochemicals, minerals and in vitro antioxidant activities of G...ijtsrd
Gongronema latifolium is primarily used as spice and vegetable as well as a herb in traditional medicine in the treatment of malaria, diabetes and hypertension. This study is aimed at providing in vitro laboratory knowledge on Gongronema latifolium leaves.Methods: Minerals were analyzed using Atomic Absorption Spectrophotometer while phyto-nutrients were screened using standard laboratory procedures. 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging and reducing power activities were determined spectrophotometrically. Usunobun Usunomena | Igwe V. Chinwe"Analysis of phytochemicals, minerals and in vitro antioxidant activities of Gongronema latifolium leaves" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-1 | Issue-4 , June 2017, URL: http://www.ijtsrd.com/papers/ijtsrd87.pdf http://www.ijtsrd.com/biological-science/biotechnology/87/analysis-of-phytochemicals-minerals-and-in-vitro-antioxidant-activities-of-gongronema-latifolium-leaves/usunobun-usunomena
Fourier-transform Infrared Analysis and In Vitro Antibacterial Activity of Or...BRNSS Publication Hub
The present research is to assess the Fourier transform infrared (FT-IR) analysis and antibacterial activities of Ormocarpum cochinchinense leaf extract using different solvents dimethyl sulfoxide (DMSO), ethyl acetate, ethanol (EtOH), methanol (MeOH), and chloroform. To investigation of FT-IR analysis and antibacterial activities become used agar well diffusion method. FT-IR vibrational bands confirmed that the fractions of O. cochinchinense had lots of biologically active compounds which include H–Bonded Phenols, alkanes, carboxylic acid, carboxylic acid, flavonoids, polyphenols, catechins, aromatics, and aliphatic amines. Antibacterial activity showed that the strongest activities had been produced by MeOH solvent reaction with all the human pathogens. This research may be concluded that MeOH solvent extract of O. cochinchinense might be a capability for the treatment of antibacterial activities.
Where buy Moringa Powder from Pakistan?
Moringa Pakistan providing the super food moringa pure to all Pakistan. You may order your details to whatsapp +92 302 4184220 or submit this form. https://forms.gle/cyb85w9zdGtQus2j9
website: https://moringa-pakistan.business.site/
Extraction of Colorant From Dragon Fruit Peel and It's Application in a baker...Salemir Hossain
This is a project or thesis proposal presentation for B.Sc Engineering level on Extraction of Colorant From Dragon Fruit Peel and It's Application in a bakery Product. If any help is needed then contact on salemirhossainjust@gmail.com
Remans - Assessing and improving nutritional diversity of cropping systemsCIALCA
Presentation delivered at the CIALCA international conference 'Challenges and Opportunities to the agricultural intensification of the humid highland systems of sub-Saharan Africa'. Kigali, Rwanda, October 24-27 2011.
Cumulative effect of modified atmospheric packaging on the textural and chemi...SukhveerSingh31
Fruits and vegetables have been consumed by humans since ancient times. Scientific
investigations have proved that an increased consumption of fruits and vegetables is known to
reduce instances of cancer and cardiovascular mortality (Bhardwaj et al., 2014)
This is a study undertaken on nutrient compositions of and processed products development from Son Tra (Docynia indica. The in-depth study of some bioactive substances of son tra fruit and its processed product development was undertaken as part of understanding existing market value chains, and identifying the necessary interventions to improve the performance of son tra.
Characterization of the Volatile Components and Antimicrobial Properties of t...Premier Publishers
The characterization of the ethanol leaf extract of Uvaria chamae was carried out with the aim of identifying and determining the chemical compounds present in the extract. Initial phytochemical results showed the presence of flavonoids, saponins, tannins. Phenols, steroids and alkaloids. Interpreted spectrum obtained from the GC-MS revealed twelve absorption peaks. Peak 1 was identified as Benzene carboxylic acid with molecular weight of 122g and molecular formula C7H6O2. Similarly, peaks 2-12 were identified as, hexadecanoic acid methyl ester, hexadecanoic acid, 11-octadecenoic acid methyl ester, phytol, 6-octadecanoic acid, octadecanoic acid, tetradecanamide. hexadecanoic acid-2,3-dihydroxpropyl ester, 9-octadecenamide, 9,12-octadecadienoyl chloride and 13-octadecanal with corresponding molecular formulas of C17H34O2, C16H36O2, C19H36O2, C20H40O, C18H34O2, C18H36O2, C14H29NO, C19H38O4, C18H35NO, C18H31ClO and C18H34O respectively. The extract inhibited the growth of some selected human pathogen; Pseudomonas aureginosa by 6mm with minimum inhibitory concentration (MIC) of 50mg/cm3. Similar results were also obtained for Candida albicans 8mm with MIC 50mg/cm3 and Trichophyton spp 4mm with MIC OF 100mg/cm3
Seaweed (Hypnea musciformis) Versus Freshwater Weed (Ipomoea aquatica): A Com...SaadAldin2
An undergraduate Thesis work accomplished in the dept. of Food Engineering and Tea Technology. This is the Defense power-point slide of the main Paper.
Analysis of phytochemicals, minerals and in vitro antioxidant activities of G...ijtsrd
Gongronema latifolium is primarily used as spice and vegetable as well as a herb in traditional medicine in the treatment of malaria, diabetes and hypertension. This study is aimed at providing in vitro laboratory knowledge on Gongronema latifolium leaves.Methods: Minerals were analyzed using Atomic Absorption Spectrophotometer while phyto-nutrients were screened using standard laboratory procedures. 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging and reducing power activities were determined spectrophotometrically. Usunobun Usunomena | Igwe V. Chinwe"Analysis of phytochemicals, minerals and in vitro antioxidant activities of Gongronema latifolium leaves" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-1 | Issue-4 , June 2017, URL: http://www.ijtsrd.com/papers/ijtsrd87.pdf http://www.ijtsrd.com/biological-science/biotechnology/87/analysis-of-phytochemicals-minerals-and-in-vitro-antioxidant-activities-of-gongronema-latifolium-leaves/usunobun-usunomena
Fourier-transform Infrared Analysis and In Vitro Antibacterial Activity of Or...BRNSS Publication Hub
The present research is to assess the Fourier transform infrared (FT-IR) analysis and antibacterial activities of Ormocarpum cochinchinense leaf extract using different solvents dimethyl sulfoxide (DMSO), ethyl acetate, ethanol (EtOH), methanol (MeOH), and chloroform. To investigation of FT-IR analysis and antibacterial activities become used agar well diffusion method. FT-IR vibrational bands confirmed that the fractions of O. cochinchinense had lots of biologically active compounds which include H–Bonded Phenols, alkanes, carboxylic acid, carboxylic acid, flavonoids, polyphenols, catechins, aromatics, and aliphatic amines. Antibacterial activity showed that the strongest activities had been produced by MeOH solvent reaction with all the human pathogens. This research may be concluded that MeOH solvent extract of O. cochinchinense might be a capability for the treatment of antibacterial activities.
Where buy Moringa Powder from Pakistan?
Moringa Pakistan providing the super food moringa pure to all Pakistan. You may order your details to whatsapp +92 302 4184220 or submit this form. https://forms.gle/cyb85w9zdGtQus2j9
website: https://moringa-pakistan.business.site/
Extraction of Colorant From Dragon Fruit Peel and It's Application in a baker...Salemir Hossain
This is a project or thesis proposal presentation for B.Sc Engineering level on Extraction of Colorant From Dragon Fruit Peel and It's Application in a bakery Product. If any help is needed then contact on salemirhossainjust@gmail.com
Remans - Assessing and improving nutritional diversity of cropping systemsCIALCA
Presentation delivered at the CIALCA international conference 'Challenges and Opportunities to the agricultural intensification of the humid highland systems of sub-Saharan Africa'. Kigali, Rwanda, October 24-27 2011.
Phytochemical Screening, Nutritional Composition and Antimicrobial Activity o...iosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Food and Nutrition Security in Africa, Investigating the role of local biodiversity in meeting nutritional requirements for complementary foods of infants and young children in Southern Benin, Celine Termote, Bioversity International
Nutritive Evaluation, Mineral Composition and Phytochemical Analysis of Leaf ...ijtsrd
Fresh green leaves of Daucus Carota was harvested and processed with a view to analyse its photochemical constituents and nutritional potentials. Proximate analysis, mineral analysis and phytochemical analysis was determining using standard analytical techniques. The nutrient composition of the leaf protein concentrate revealed Moisture content 8.69±0.03 g 100g, crude protein 18.38±0.08 g 100g, crude fibre 14.81±0.02 g 100g, crude fat 5.69±0.23 g 100g, Ash 19.69±0.02g 100g , and NFE 32.74±0.43 g 100g.The mineral analysis of the sample shows the concentrations of the elements in the following descending order of magnitude Ca P Mg Na Fe K Zn Cu Mn Se while Hg was not detected in the sample. The photochemical constituents also revealed Tanin 3.66±0.16 mg 100g, Saponin 4.34±0.06 mg 100g, Alkaloid 10.49±0.02 mg 100g, Oxalate 6.89±0.04 mg 100g, Phytate 14.62±0.05 mg 100g, Phenolics 3.75±0.82 mg 100g, Flavonoids 3.62±0.43 mg 100g. High Concentration of Calcium and Phosphorus indicates that Daucus carota leaf protein concentrates is fit for consumption by children, pregnant women and weaned infants for good teeth and bone formation. The constituents of Daucus carota leaf protein concentrates present values of significant nutritional benefits and can find application in various food additives, binders, flavonoids and vitamins. It could also be used as additives in some drugs if the active compounds present in it could be analysed to detect its suitability. Sodamade, A. | Raimi, S. M. | Owonikoko, A. D. | Adebimpe, A. T. "Nutritive Evaluation, Mineral Composition and Phytochemical Analysis of Leaf Protein Concentrates of Daucus carota" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-5 , August 2019, URL: https://www.ijtsrd.com/papers/ijtsrd26427.pdfPaper URL: https://www.ijtsrd.com/chemistry/analytical-chemistry/26427/nutritive-evaluation-mineral-composition-and-phytochemical-analysis-of-leaf-protein-concentrates-of-daucus-carota/sodamade-a
Phytochemical Screening of Justina Gendarussa Burm F. Panhauli Leaf Extractijtsrd
This study was conducted to determine the secondary metabolites present in the leaf extract of Justina gendarussa Burm. F. locally known as Panhauli. These metabolites include alkaloids, anthraquinones, leucoanthocyanin, phenolic compound, saponin, steroid, tannin, and terpenoids. Results of the study showed that there was a 30 of extract yielded for every 100 grams of plant leaf. Physical properties of the extract of Panhauli leaves showed that it has a 103. 3oC boiling point, brown color and a pleasant odor, its density was 1.02g mL and a neutral pH. Further physical test showed it is miscible in methanol and water, and immiscible in chloroform and dichloromethane which signifies that the Panhauli leaves extracts has a polar components. Finally phytochemical tests of the Panhauli extract showed the confirmed positive results in alkaloid and saponin only, other secondary metabolites mentioned here in this study were deemed negative. Cristelyn G. Losaura | Charito V. Mollejon ""Phytochemical Screening of Justina Gendarussa Burm F. (Panhauli) Leaf Extract"" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-4 , June 2019, URL: https://www.ijtsrd.com/papers/ijtsrd23749.pdf
Paper URL: https://www.ijtsrd.com/other-scientific-research-area/other/23749/phytochemical-screening-of-justina-gendarussa-burm-f-panhauli-leaf-extract/cristelyn-g-losaura
Comparative analysis of mineral constituents of ethanol leaf and seed extract...PUBLISHERJOURNAL
Medicinal plants contain numerous biologically active compounds such as minerals which have physiological actions on the human body. This work was designed to analyse the mineral composition of Datura stramonium (D. stramonium) leaf and seed extracts. Dry leaves and seeds of D. stramonium were pulverized. The powdered sample was used for the extraction using 98% ethanol. Standard methods were used in the determination of the mineral contents. The study result showed the order of trace minerals composition of D. stramonium leaves and seed were as follows: Fe>Cu>Mn>Zn>Ni>Co and Cu>Fe>Zn>Mn>Ni>Co, respectively. The result showed higher value of trace minerals in D. stramonium leaves than the seed. There were significant difference (p<0.05) in the trace elements levels of the leaves and seeds with the leaves having higher mineral levels than the seed. The result also showed that macro minerals of D. stramonium leaves and seed were in this order: Mg>Ca>K>P. D. stramonium leaves had higher quantities of macro mineral than the seeds. D. stramonium leaves and seed used for this study have proved to be very important in drug research and development, because of the mineral composition that are present in appreciable amount. These minerals could contribute to the various pharmacological attributes of this plant. However, further research is necessary to determine other bioactive constituents present in this plant and their mechanism of action.
Keywords: Minerals, Datura stramonium, trace elements, macro mineral, medicinal plants, active compounds
The study of physiochemical properties of fresh Rosemary leaves powder and also the preparation of tea from standard green tea. Powder of Rosemary leaves is prepared by sun drying for duration of 3 4 hours, followed by grinding into fine particles size. Dried leaves are subjected to proximate analysis. Dried leaf powder reveals a moisture content of 6.41 , total ash at 5.52 , and crude fiber at 25.19 . The physicochemical properties disclose that sorbic acid is absent. Three formulations T0, T1, and T2 were created by varying the amounts of rosemary leaves 1.45gm, 1.50gm, and 1gm green tea ratio 1.45gm, 1gm, and 1.50gm rosemary green tea T2 was accepted on the basis of sensory characteristics like taste, texture, and flavor and overall acceptability. The selected sample was assessed from proximate analysis and microbial tests. Khushi Amit Patel | Dhanya Joseph "Development of Rosemary Induces Green Tea" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-7 | Issue-2 , April 2023, URL: https://www.ijtsrd.com.com/papers/ijtsrd53905.pdf Paper URL: https://www.ijtsrd.com.com/home-science/food-and-nutrition/53905/development-of-rosemary-induces-green-tea/khushi-amit-patel
PROXIMATE AND MINERAL COMPOSITION OF Pentadiplandra brazzeana STEM BARKSubmissionResearchpa
Plants are the cheapest and indispensable constituents of human diets supplying the body nutrients (carbohydrates, protein, fats, amino acids, vitamins) necessary for growth and body development. Therefore, this work was designed to examine the proximate and mineral composition of Pentadiplandra brazzeana stem bark (PBSB). Proximate composition of PBSB revealed the presence of 8.75 % moisture, 91.25 % dry matter, 6.43 % crude protein, 41.03 % crude fibre, 5.70 % ether extract, 12.11 % ash, 17.82 (g/100 g) carbohydrates, 0.47 % total reducing sugar and 632.2 Kj/100g energy respectively. Results on mineral analysis shows that PSSB is abundant in calcium (73.84 mg/100g) followed by phosphorus (41.55 mg/100g), magnesium (32.56 mg/100g), sodium (28.11 mg/100g), zinc (17.56 mg/100g), manganese (10.88 mg/100g), potassium (9.47 mg/100g) and copper (2.33 mg/100g). In order of mineral abundance in PBSB Ca ˃ phosphorus ˃ magnesium ˃ sodium ˃ zinc ˃ manganese ˃ potassium ˃ copper. It was concluded that PBSB is low in protein, energy and some minerals (copper and potassium). by Alagbe, J.O, Shittu, M. D, Bamigboye Samson. O and Oluwatobi, A.O 2020. PROXIMATE AND MINERAL COMPOSITION OF Pentadiplandra brazzeana STEM BARK. International Journal on Integrated Education. 3, 11 (Nov. 2020), 114-118. DOI:https://doi.org/10.31149/ijie.v3i11.844. https://journals.researchparks.org/index.php/IJIE/article/view/844/805 https://journals.researchparks.org/index.php/IJIE/article/view/844
The leaf, stem and flowers of Luffa aegyptiaca were screened for their phytochemical and physicochemical properties. The phytochemical evaluation of the leaf, stem and flowers revealed the presence of saponins, tannins and cardiac glycosides. Alkaloids were only present in the flowers. Cyanogenetic glycosides and phlobatannin were absent in the leaf and stem respectively. The nutrient value shows that the leaves contain 10.01% of moisture, 0.78% of crude protein, 2.40% of lipids, 14.61% of crude fibre, 3.65%of ash and 48.02% of carbohydrate. The stem contain7.02% of moisture, 3.01% of crude protein, 4.50% of lipids, 8.10% of crude fibre, 2.50% of ash and 61% of carbohydrate. While the flowers contain4.01% of moisture, 0.5% of crude protein, 5.1% of lipids, 7.01% of crude fibre, 1.5% of ash and 45.1% of carbohydrate. These findings prove that Luffa aegyptiaca contains bioactive compounds that may be useful in nutrition and explains its popular use in traditional medicine in Nigeria.
Phytochemical, Proximate and Nutrient Analysis of Cassia Tora Seedsinventionjournals
Recently Proximate analysis and phytochemical analysis and nutrient analysis of seeds of Cassia tora L. from the campus of Government Vidharbha Institute of Science and Humanity, Amravati had been investigated. The seeds sample contained tannin, saponin, protein, steriods, terpenoids, carbohydrate, alkaloids, flavonoids and glycosides. Proximate analysis of moisture, ash, fat amd mineral analysis of calcium, magnesium, iron, nitrogen and solubility were check. The values of it is moisture (56%), cold water (52%), hot water (54%), 1%NaOH (43%), 1%HCl (61%), benzene +alcohol (35%), ash content (17%). These results indicate that the seeds of these Cassia tora L. contains mineral and nutrients elements that will be useful in nutrition. Also the existence of some phytochemicals like tannin, saponin and steroids illustrated medicinal action of the plant in its therapeutic uses. The result of their phytochemcial screening could justify the observed activities and validate their use in herbal medicine.
Phytochemical, Antioxidant and Antibacterial Studies on Bambusa arundinacea a...SUS GROUP OF INSTITUTIONS
This study was formulated to check the phytochemical, antioxidant, antibacterial potential of
Bambusa arundinacea (Bamboo) and Mangifera indica (Mango) trees. Aqueous, ethanolic and
methanolic extracts were prepared from leaves of former and stem bark of later. The phytochemical
screening of the extracts showed the presence of various bioactive compounds such as
carbohydrates, flavonoids, saponins and proteins in B. arundinacea, alkaloids, flavonoids, tannins,
saponins, steroids and cardiac glycosides in M. indica. Total phenolic concentration and
percentage of free radical scavenging activity was more in ethanolic extracts of B. arundinacea and
M. indica followed by methanolic extracts and aqueous extracts. Highest percentage of ferric
reducing antioxidant power was found in ethanolic extracts and lowest in aqueous extracts indicates
that ethanolic extracts has more antioxidant potential than the other two extracts. Ethanolic extracts
of both plants had higher inhibition on the tested Gram positive (B. subtilis & S. aureus) as well as
Gram negative (E.coli & P. aeruginosa) bacteria evidenced from the zones of inhibition. M. indica
showed more therapeutic potential as compared to B. arundinacea and ethanolic as well as
methanolic extracts of both the tested plants were more effective than aqueous extracts due to better
extraction power of organic solvents. Overall study indicates that B. arundinacea and M. indica are
potential source of natural antioxidants, phytochemicals and antibacterials that can be used for the
development of novel drugs and may represent new source of antimicrobials with stable, biologically
active components that can establish a scientific base for further use in modern medicines.
Phytochemical composition of Datura stramonium Ethanol leaf and seed extracts...PUBLISHERJOURNAL
The study of natural products has given rise to the discovery of a variety of useful drugs for effective management of several diseases. Phytochemical constituents of plants are fingered to be biologically active compounds responsible for different activities such as analgesic, anti-inflammatory, antioxidant, antimicrobial, antifungal and anticancer. Thus, assessment of phytochemical constituents of plants is of utmost importance in medicine. There is however paucity of information on the comparative assessment of leaves and seeds of Datura stramonium (D. stramonium). Therefore, the present study was carried out to compare the phytochemical constituents of D. stramonium leaves and seeds. Ethanol was used for the extraction using standard method. Phytochemical screening was performed using various quantitative methods. The ratio of leaves versus seeds of phytochemical quantitative analysis was as follows: saponins (65.25: 34.25 mg/g), alkaloids (32.10: 71.28 mg/g), flavonoids (44.24: 30.12 mg/g), terpenoids (32.68:30.20 mg/g), tannins (31.25:26.62), respectively. This implies that saponins were dominant compounds followed by flavonoids and terpenoids in leaves while alkaloids were the dominant phytochemical in seeds. Based on our results, it is concluded that leaf and seed extracts of D. stramonium have varying concentrations of phytochemicals with the seed having higher concentration of alkaloids justifying why the seed is more toxic than the leaves while leaf extract had highest concentration of saponins and flavonoids giving credence to the antioxidant property of the leaves. Good quantity of various phytochemicals in this plant justifies its traditional use in the treatment of various diseases.
The word “banana” is a general term comprising a number of species or hybrids in the genus Musa of family Musaceae. Many studies reported that banana is a good source of phenolic compounds, which are having potential health benefits in human beings. The phytochemical composition of eight banana varieties was investigated. Quantitative estimation of phytochemicals revealed that highest alkaloid and tannin content was noticed in variety Nendran (3.76% and 4.40 mg/100g respectively). Whereas, variety Rasakadali exhibited highest flavonoid content (9.49 mg/100). Total phenol and saponin content was found higher in variety Kadali (0.82% and 11.6 mg/100g respectively).
Phytochemical Analysis of some Macrophytes of Lake Kondakarla, Visakhapatnam ...iosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
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Comparative analysis of the phytochemical and nutrient
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Comparative Analysis of the Phytochemical and Nutrient
Evaluation of the Seeds and Leaves of Plukenetia
conophora plant
P. B. Ayoola1
, O. O. P. Faboya2
and O. O. E. Onawumi2
1
Department of Science Laboratory Technology, Ladoke Akintola University of Technology, P.M.B.4000,
Ogbomoso, Oyo State, Nigeria.
E-mail: pbayoola@yahoo.com
2
Department of Chemistry, Ladoke Akintola University of Technology, P.M.B.4000, Ogbomoso, Oyo State,
Nigeria.
2
Department of Chemistry, Ladoke Akintola University of Technology, P.M.B.4000, Ogbomoso, Oyo State,
Nigeria.
E-mail: estherdr@rocketmail.com
ABSTRACT
Samples of Plukenetia conophora fresh seeds and fresh leaves were analysed for Proximate composition,
secondary metabolites, Vitamins and Mineral constituents. The result of proximate analysis shows that the fresh
seeds and leaves respectively contained 46.03±0.03% and 29±0.71% moisture, 4.28±0.01% and 5.63±0.08%
fat, 7.34±0.71% and 14.92±0.04% fibre, 21.65±0.5% and 16.62±0.30% protein, 5.27±1.35% and 12.89±0.02%
Ash, 18.10±0.01% and 20.94±0.01% carbohydrate. The phytochemical screening and subsequent quantification
of the seed and leaves revealed the presence of tannin, 0.089±0.01mg/kg and 0.560±0.01mg/kg, alkaloid,
2.380±0.2mg/kg and 2.670±0.02, gallic acid, 0.024±0.01mg/kg and 0.180±0.02mg/kg. There was no traces of
saponin and anthraquinones in the fresh seed, while flavonoids, cardiac glycosides, anthraquinones, ellagic acid
and caffeinic acid were absent in both part of the plant samples. The mineral analysis revealed the constituents to
be K, 6250±0.2mg/kg and 15937±0.02, Na, 4830±0.1mg/kg and 7980±0.01mg/kg, Ca, 4337.5±0.08mg/kg and
18700±0.02mg/kg, Mg, 1711.25±0.8mg/kg and 1766.25±0.1mg/kg, Fe, 110±0.04mg/kg and 4610±0.10mg/kg,
Zn, 40.10±0.10mg/kg and 61.15±0.08mg/kg, Mn, 22.00±0.01mg/kg and79.50±0.03mg/kg, Cu, 16.45±0.03mg/kg
and 8.60±0.10mg/kg for the fresh seed and leaves respectively.
Vitamin composition results showed that the fresh seed and leaves respectively contained Thiamine (B1)
0.06±0.008μg/100g and 0.29±0.01µg/100g, Riboflavin (B2) 0.02±0.008μg/100g and 0.34±0.01µg/100g, Niacin,
0.05±0.02μg/100g and 0.12±0.3µg/100g, Cyanocobalamin (B12),0.12±0.1μg/100g and 0.23±0.03µg/100g.
Ascorbic acid (C) 4.15±0.02mg/100g and 16.28±0.04mg/100g, tocopherol (E), 122.57±0.20µg/100g and
2.67±0.008µg/100g. The results proved that Plukenetia conophora fresh seeds and leaves can serve as foods and
could also be potential sources of useful drugs formulation.
Key words: Plukenetia, conophora, phytochemical, nutrient,
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INTRODUCTION
Plukenetia conophora , formerly called Tetracarpidium conophorum (family Euphorbiaceae) is found
in Nigeria and Cameroon. It is a climbing shrub 10-20ft long, it is known in the Southern Nigeria as ukpa (Igbo),
Western Nigeria as awusa or asala (Yoruba). It is found in Uyo, Akamkpa, Akpabuyo, Lagos, Kogi , Ajaawa-
Ogbomoso and Ibadan. This plant is cultivated principally for the nuts which are cooked and consumed as
snacks [20]. A bitter taste is usually observed upon drinking water immediately after eating the nuts. This could
be attributed to the presence of chemical substances such as alkaloid. [3] reported the presence of oxalate,
phylates and tannin in the raw Tetracarpidium conophorum nuts. [9] also reported the proximate composition,
ascorbic acid and heavy metal contents of the nut. The work done by [25] revealed the presence of amino acid
and fatty acid components of the nut and the use of its leaf juice for the treatment of prolonged and constant
hiccups.
The impact of traditional processing on the nutrient and sensory qualities of the nut. the methods of
processing the Tetracarpidium conophorum nuts [21], and in processing waste in livestock feed formulation
have been reported. [10] compared the level of toxicant in the seeds of Terminalia catappa (Indian almond) and
Coula edulis (African walnut). They also determined the lipid content of the fresh seed. [4] reported the
phytochemical and nutrient evaluation of the T.conophorum root.
Though the nuts are generally eaten in Nigeria as snack not minding is medicinal properties. Some data are
available on the phytochemical constituents of the seeds but the leaves are of little or not available. The objective
of this work therefore is to compare and evaluate the secondary metabolites, proximate, mineral contents and
vitamin compositions of Plukenetia conophora seeds and leaves in order to ascertain their possible usefulness as
food and in formulation of drugs.
MATERIALS AND METHODS
Fresh seeds and leaves samples of study plant were collected at Oshu village in Oko area, Alagbayen
farm, Surulere Local Government Area of Oyo State, Nigeria. The materials were washed, cut into small pieces
to facilitate dryness, and air-dried for fourteen days. The dried samples were ground into fine powder and stored
in an air tight bottle and stored in the desiccator prior to analysis.
Proximate analysis:
Moisture content was determined by drying to constant weight at 100-105o
C in an oven, ash content by
ignition at 550o
C in a muffle furnace for 4hr, oil content by soxhlet extraction with hexane as solvent, protein by
the kjeldahl method, and crude fibre by the acid and alkaline digestive methods all described by [15]. The
carbohydrate content was estimated by difference, subtracting the sum of water, protein, fat, crude fibre and ash
percentages from one hundred.
Phytochemical analysis:
The phytochemical screening was done on the sample using methods as described by [2, 27] Alkaloids were
extracted using a slightly modified method of [17]. Here, the dried sample was homogenized and the alkaloid
extracted from 10g of the sample for 4h using 20% v/v acetic acid in ethanol. The extract was filtered to remove
cellulose debris and then concentrated to about one quarter of the original volume. One percent NH4 OH was
added drop wise until a precipitate occurred. The crude alkaloid was dried to constant weight in an oven and the
percentage alkaloid calculated.
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Mineral analysis:
The [2] method was used for the determination of minerals in the test sample. Calcium, sodium,
potassium, magnesium were determined by flame photometric method while iron, zinc, manganese, copper and
chromium were determined by atomic absorption spectrophotometric method.
Vitamin analysis:
The composition of the water-soluble vitamins such as thiamine (B1), riboflavin (B2), niacin (B3),
cyanocobalamin (B12) were determined by the method of scalar analyzer (2000) While ascorbic acid (vitamin c)
content was determined by the method of [2]. [5] was used for the determination of tannins, while saponin was
analyzed using that of [26].
RESULTS AND DISCUSSION
Based on the results of the proximate composition of the seeds and leaves as shown in Table 1, the moisture
content was much in the seed than the leaves which is an indication that the leaves could have longer shelf life
than the seeds. The fat content in the leaves was higher (5.63 ±0.08%) than that of the seed and can be compared
to the reported value by [9]. The higher ash content of the leaves is an indication of the presence of high
inorganic materials than the seed. Leaves contained more nutrients than the seed except the protein content
where the seeds have higher value but lower than the reported value [9].
The result for the mineral analysis (Table 2) shows that leaves of Plukenetia conophora plant contained higher
values of minerals except copper that was higher in the seed. Both parts are good sources of important minerals
that are useful to the human body. This result become so important when the usefulness of such minerals like Ca,
Mg, Na, K in the body are considered and their usefulness in bone management. The presence of copper is
responsible for the absorption of iron, it is therefore often seen with iron naturally. Copper is important for
cellular defence and protection of the mucous membrane, antianaemic and essential for the formation of
haemoglobin from iron [6]. Manganese is necessary for the functioning of the pituitary gland, the pineal gland
and the brain, it promotes hepatorenal function, combat anaemia and also essential for growth.
The presence of zinc is an indication that the seed and leaves may have some effect on the nerve function
and male fertility. It is important for normal sexual development, especially for the development of testes and
ovaries, it is also essential for reproduction. Zinc stimulates the activity of vitamins, formation of red and white
corpuscles [6], healthy functioning of the heart and normal growth [11].
The phytonutrients present in the seed and leaves as shown in Table 3 and 4, shows that leaves were rich
in alkaloids, saponin, tannin and anthraquinones while seeds contained less alkaloids and traces of tannins,
saponin and anthraquinones were absent in the seed. Alkaloids are the most efficient plant substances used
therapeutically. Pure isolated alkaloids and the synthetic derivatives are used as the basic medicinal agent
because of their analgesic, antispasmodic and bacterial properties. The presence of tannins in both seed and leaf
support their strong use for healing of haemorrhoids, frost bite and varicose ulcers in herbal medicine [13].
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PLATE 1.0: P.CONOPHORA LEAVES AND A POD OF THE NUT
PLATE 2: WALNUT SEEDS IN THE POD: SINGLE AND DOUBLE PODS
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PLATE 2: WALNUT SEEDS RELEASED FROM THE POD
PLATE 3: WALNUT SEED RELEASED FROM THE SHELL
Table 5 shows the vitamin composition of both the seed and leaves. The seed contained higher amount of
Vitamin E than the leaf, while the leaf contained higher amount of vitamin C than the seed (Both vitamins are
antioxidants). Vitamin B groups are present in the leaf in trace amount yet more than that of the seed.
Considering the importance of all these mentioned vitamins, vitamin C can be used for the treatment of common
cold and other diseases like prostrate cancer [23, 24]. Higher amount of vitamin E in the seed supports the use of
the seed in southern Nigeria ethnomedicine as a male fertility agent [1].
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Other vitamins though in trace amount are essential for body metabolism. There is also an interesting
ability of ascorbic acid as an antioxidant, to prevent or at least minimize the formation of carcinogenic
substances from dietary material. Deficiency of ascorbic acid is associated with pains in the joint and defect in
skeletal calcification, anaemia, manifestation of scurvy haemorrhage from mucous membrane of the mouth and
gastrointestinal track [12].
CONCLUSION
The present study compared the phytochemicals, proximate, vitamins and minerals composition of
Plukenetia conophora (African walnut) seeds and leaves. This partly shows that the leaf is also useful and
important as the seed and can equally be used as food and drug in herbal medicine or drug formulation.
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of Tetracarpidium conophorum (Nigerian walnut) Root. International Journal of Research and Review in
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[8] D’ Amelio F.S, Botanical; A phytocosmetic Desk Reference, Boca Raton, FL,CRC Press. p: 209, 1999.
[9] Edem, Christopher A. Dosunmu, Miranda,I. and Bassey Francesca, I. Determination of Proximate
Composition, Ascorbic Acid and Heavy Metal Content of African Walnut (Tetracarpidium conophorum).Pak .J.
Nutrit., 8: 225-226, 2009.
[10] Ekpo, A. S. and Eddy, N. O. Comparative Studies of The Level of Toxicant in the Seeds of Terminalia
catappa (india almond) and Coula edulis (African walnut). Chem Class Journal , 2, p: 74, 2005.
[11] Elizabeth, K., Immense help from Natures workshop. 1st
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[12] Hunt, S., Goff ,J.L. and Holbrook, J., Nutrition Principles and Chemical Practices. John Wiley and Sons.
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[13] Igboko, D.O., Phytochemical studies on Garcinia kola Heckel. M.sc. Thesis. University of Nigeria
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[14] James, N. Roitman. Volatile Components of Green Walnut Husks. J. Agric. FoodChem. 48(7) pp: 2858-
2861, 2000
[15] Lees .R., Food Analysis: Analytical and Quality Control Methods for the Food Manufacturer and Buyer.
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[16] Maduiyi, I., Biochemical and pharmacological studies of active principles of the seeds of Garcinia kola
Heckel. M.sc. Thesis University of Nigeria, Nsukka. , pp: 108, 1983.
[17] Maxwell, A., Seepers, M.P., and Mootoo, D.R., Aminospirosola NE steroidal alkaloids from solanum
trists. J .Nat. Product, 56, pp: 821-825, 1995.
[18] Nwaoguikpe, RN, Ujowundu, CO and Wesley, B. Phytochemical and Biochemical Compositions of
African walnut (Tetracarpidium conophorum). Journal of Pharmaceutical and Biomedical Sciences. 20(9):1-4.
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[19] Okafor, B.B., Chemical Studies on some Nigerian Foodstuffs. Nigeria Cone Press Ltd., London, 1988.
[20] Oke, O.L., Leaf Protein Research in Nigeria Ibadan. University of Ibadan Press, Nigeria. 1995
[21] Okpero, A.O., The Nutritive Value of Conophor Seed. University of Ibadan Press, Nigeria. 2001.
[22] Okwu, D.E., Evaluation of Chemical Composition of Indigenous Spices and Flavouring Agents. Global J.
Pure Appl. Sci., pp: 458-459, 2001.
[23] Okwu, D.E.,and Okeke, O.,(2003). Phytochemical Screening and Mineral Composition of Chewing Sticks
in South Eastern Nigeria. Global J. Pure Appl. Sci., 9: 235-238. 2003.
[24] Okwu, D.E., Okwu, M.E., Chemical Composition of Spondia mombin plants .J. Sustain. Agri. Environ., 6:
140-147, 2004.
[25] Oyenuga, V.A., Nigeria Food and Feeding Stuffs Ibadan. University Press, Ibadan. 1997
[26] Peng, J.P. and Kobayasli, H. Novel Furastonol Glycosides from Allium Anacros Tenon Plant Media., 6:
58-61, 1995
[27] Sofowara, A., (1993). Medicinal Plants and Traditional Medicine in African. Spectrum book. 2nd
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[28] Stray, F., The Natural guide to medicinal herbs and plants. Tiger Book International, London, pp: 12-16,
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[29] Tapsell, L. C, Gillen, I. J., Patch, C. S. Including Walnuts in a low-fat/Modified-fat diet improves HDL
cholesterol-to-total cholesterol ratios in patients with type 2 diabetes care. 27 (12). Pp: 2777-2783, 2004
[30] Tidjani Amza, Issoufou Amadou, Mohammed, T. Kamara, Kexue Zhu and Huiming Zhou. Chemical and
Nutrient Analysis of Gingerbread Plum (Neocarya macrophylla) Seeds. Advance Journal of Food Science and
Technology, 2(4):191-195, 2010.
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RESULTS AND DISCUSSION
Table 1: Proximate composition of the fresh seeds and leaves of Plukenetia conophora
(Tetracarpidium conophorum) plant
Component Fresh seeds Leaves Literature
Edem et al, 2009
Moisture content (%) 46.03±0.30 29±0.71 48.70
Crude Fat content (%) 4.28±0.01 5.63±0.08 6.21
Crude Protein content (%) 21.65±0.5 16.62±0.30 35.22
Crude Fibre content (%) 7.34±0.71 14.92±0.04 3.34
Ash content (%) 5.27±1.35 12.89±0.02 2.03
Carbohydrate content (%) 18.10±0.01 20.94±0.01 53.20
Total energy value (J) 197.52±2.13 200.91±1.96 409.57±0.5
Results are means (±SD) of three determinations
Table 2: Mineral compositions of the raw seeds and leaves of the Plukenetia conophora plant on a dry
weight basis.
Mineral
Mg/Kg
Fresh seeds
Mg/kg
Leaves
Mg/kg
Literature
Tidjain et al, 2010
Potassium 6250.00±0.2 15937.00±0.02 12500.59±0.53
Sodium 4830.00±0.1 7980.00±0.01 1360.03±0.30
Calcium 4337.5±0.08 18700±0.02 6669.99±0.10
Magnesium 1711.25±0.8 1766.25±0.1 20999.65±0.65
Iron 110.00±0.04 4610.00±0.10 166.06±0.06
Zinc 40.10±0.10 61.15±0.08 110.84±0.79
Manganese 22.00±0.01 79.50±0.03 22.66±0.11
Copper 16.45±0.03 8.60±0.10 45.08±0.10
Results are means (±SD) of three determinations
Table 3: Phytochemical screening of the fresh seeds and leaves of Plukenetia conophora plant
Constituent Fresh seeds Leaves Literature
(Ajaiyeoba and
Fadare,2006)
Alkaloids +++ +++ ++
Flavonoids -ve -ve -ve
Cardiac glycosides -ve -ve -ve
Saponins -ve +++ ++
Tannins +++ ++ ++
Anthraquinones -ve + +
Gallic acid + ++ -ve
Ellagic acid -ve -ve -ve
Caffeic acid -ve -ve -ve
KEY: -ve= absent
+ = present in a minute amount
++ = present in a moderate amount
+++ = present in an appreciable amount
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Table 4: Quantitative estimates of secondary metabolites present in the fresh seeds and leaves of the
Plukenetia conophora plant
Constituent Fresh seed Leaves Literature
Nwaoguikpe et al, 2012.
Alkaloids 2.380±0.2 2.670±0.02 0.41±0.01
Flavonoids 0.000 0.000 2.70±0.1
Cardiac glycosides 0.000 0.000 0.000
Saponins 0.000 1.080±0.01 5.03±0.01
Tannins 0.089±0.01 0.560±0.01 0.51±0.2
Anthraquinones 0.000 0.130±0.01 -
Gallic acid 0.024±0.01 0.180±0.02 -
Ellagic acid 0.000 0.000 -
Caffeinic acid 0.000 0.000 -
Results are means (±SD) of three determinations
Table 5: Vitamin compositions of the fresh seeds and leaves of Plukenetia conophora plant on a dry
weight basis
Vitamin Fresh seeds Leaves Literature
Nwaoguikpe et al,2012
Ascorbic acid (C)
mg/100g
4.15±0.02 16.28±0.04 17.57±0.02
Tocopherol (E)
μg/100g
122.57±0.20 2.67±0.008 0.27±0.02
Thiamine (B1)
μg/100g
0.06±0.008 0.29±0.01 0.12±0.01
Riboflavin(B2)
μg/100g
0.02±0.008 0.34±0.01 0.13±0.01
Niacin (B3)
μg/100g
0.05±0.02 0.12±0.3 2.91±0.10
Pantothenic acid (B5)
μg/100g
0.00 0.00 -
Pyridoxine(B6)
μg/100g
0.00 0.00 -
Folic acid (B9)
μg/100g
0.00 0.00 -
Cyanocobalamin (B12)
μg/100g
0.12±0.1 0.23±0.03 -
Results are means (±SD) of three determinations
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